Rh1DG463500

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001969
Physical Location & Seq
Reverse (-)
66598178 .. 66598655
478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG463500.1

Sequence Viewer

Length: 402 bp
ATGGAATGGGCTCTAAACGTAAGAGTTAATGGGGAGCTTGGCAATTTGGATCCTTTGTATATAAATGTTCAGAAAGCTGAGTACGAGAAATGCCATTCCGATAATCCCATTTTCAACTTTAACGATGGTGACTCGGTTTTCAAATTGGATCGACCCGGTTCGTTCTACTTCATTAGAGGGACTGCCGGACACTGCGAGAAAGGGCAAAAAATGATTGTCAAAGTCTTGGGATCACCGGCTGCTGGAACAGAAAGCCCACCACCTGCTCAATCAGCAAACCAGAATGCAACTGAATCACCAGATCATCATGACATGAAGAACAATGCAGTTGCAATGCATGCTGTAGCTGCCATCTCTTTTACAAGTTCTGTAATATCATTTCTTGGGGTCTTTTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

133

Amino Acids

14.52

Weight (kDa)

5.64

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 22 - 69 1.4e-10 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 271
Acc36I ACCTGC 1 cut(s) 271
AclWI GGATC 4 cut(s) 44, 57, 156, 238
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 242
AgsI TTSAA 2 cut(s) 115, 142
AluBI AGCT 3 cut(s) 37, 77, 347
AluI AGCT 3 cut(s) 37, 77, 347
AlwI GGATC 4 cut(s) 44, 57, 156, 238
ApeKI GCWGC 2 cut(s) 239, 347
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 3 cut(s) 140, 225, 288
BamHI GGATCC 1 cut(s) 49
BanII GRGCYC 1 cut(s) 13
BbvI GCAGC 2 cut(s) 226, 334
BccI CCATC 2 cut(s) 119, 359
BcnI CCSGG 1 cut(s) 156
BfaI CTAG 1 cut(s) 400
BfmI CTRYAG 1 cut(s) 342
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 2 cut(s) 240, 348
BlsI GCNGC 2 cut(s) 241, 349
Bme1390I CCNGG 1 cut(s) 156
BmiI GGNNCC 1 cut(s) 51
BmrFI CCNGG 1 cut(s) 156
BpuMI CCSGG 1 cut(s) 156
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse118I RCCGGY 1 cut(s) 235
Bse3DI GCAATG 1 cut(s) 339
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 1 cut(s) 69
BseXI GCAGC 2 cut(s) 226, 334
BsiSI CCGG 3 cut(s) 156, 186, 236
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 193
BsmI GAATGC 1 cut(s) 289
Bsp1286I GDGCHC 1 cut(s) 13
Bsp143I GATC 4 cut(s) 49, 148, 230, 301
BspCNI CTCAG 1 cut(s) 70
BspHI TCATGA 1 cut(s) 307
BspLI GGNNCC 1 cut(s) 51
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 4 cut(s) 44, 57, 156, 238
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 235
BssAI RCCGGY 1 cut(s) 235
BssMI GATC 4 cut(s) 49, 148, 230, 301
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 1 cut(s) 78
BstKTI GATC 4 cut(s) 52, 151, 233, 304
BstMBI GATC 4 cut(s) 49, 148, 230, 301
BstMWI GCNNNNNNNGC 3 cut(s) 272, 338, 347
BstNSI RCATGY 1 cut(s) 341
BstSCI CCNGG 1 cut(s) 154
BstSFI CTRYAG 1 cut(s) 342
BstV1I GCAGC 2 cut(s) 226, 334
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BtsI GCAGTG 1 cut(s) 190
BtsIMutI CAGTG 1 cut(s) 190
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 1 cut(s) 339
CciI TCATGA 1 cut(s) 307
Cfr10I RCCGGY 1 cut(s) 235
Csp6I GTAC 1 cut(s) 82
CviAII CATG 3 cut(s) 308, 313, 338
CviJI RGCY 6 cut(s) 11, 37, 77, 239, 255, 347
CviKI_1 RGCY 6 cut(s) 11, 37, 77, 239, 255, 347
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 1 cut(s) 78
DpnI GATC 4 cut(s) 51, 150, 232, 303
DpnII GATC 4 cut(s) 49, 148, 230, 301
Eco24I GRGCYC 1 cut(s) 13
EcoT22I ATGCAT 1 cut(s) 339
EcoT38I GRGCYC 1 cut(s) 13
FaeI CATG 3 cut(s) 311, 316, 341
FaiI YATR 5 cut(s) 60, 62, 309, 314, 339
FaqI GGGAC 1 cut(s) 193
FatI CATG 3 cut(s) 307, 312, 337
Fnu4HI GCNGC 2 cut(s) 240, 348
FriOI GRGCYC 1 cut(s) 13
Fsp4HI GCNGC 2 cut(s) 240, 348
FspBI CTAG 1 cut(s) 400
GluI GCNGC 2 cut(s) 240, 348
HapII CCGG 3 cut(s) 156, 186, 236
Hin1II CATG 3 cut(s) 311, 316, 341
HinfI GANTC 2 cut(s) 131, 293
HpaII CCGG 3 cut(s) 156, 186, 236
HphI GGTGA 3 cut(s) 140, 225, 288
Hpy188I TCNGA 2 cut(s) 72, 100
Hpy188III TCNNGA 1 cut(s) 308
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 4 cut(s) 287, 326, 332, 337
HpyF10VI GCNNNNNNNGC 3 cut(s) 272, 338, 347
HpyF3I CTNAG 1 cut(s) 78
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 3 cut(s) 311, 316, 341
Kzo9I GATC 4 cut(s) 49, 148, 230, 301
LmnI GCTCC 1 cut(s) 34
LpnPI CCDG 7 cut(s) 169, 199, 228, 249, 276, 293, 312
Lsp1109I GCAGC 2 cut(s) 226, 334
MaeI CTAG 1 cut(s) 400
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 128
MalI GATC 4 cut(s) 51, 150, 232, 303
MboI GATC 4 cut(s) 49, 148, 230, 301
MboII GAAGA 2 cut(s) 328, 388
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 13
MluCI AATT 2 cut(s) 43, 143
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 1 cut(s) 170
Mph1103I ATGCAT 1 cut(s) 339
MseI TTAA 2 cut(s) 27, 120
MspI CCGG 3 cut(s) 156, 186, 236
MspR9I CCNGG 1 cut(s) 156
Mva1269I GAATGC 1 cut(s) 289
MwoI GCNNNNNNNGC 3 cut(s) 272, 338, 347
NciI CCSGG 1 cut(s) 156
NdeII GATC 4 cut(s) 49, 148, 230, 301
NlaIII CATG 3 cut(s) 311, 316, 341
NlaIV GGNNCC 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 128
NsiI ATGCAT 1 cut(s) 339
NspI RCATGY 1 cut(s) 341
PaeI GCATGC 1 cut(s) 341
PagI TCATGA 1 cut(s) 307
PaqCI CACCTGC 1 cut(s) 271
PctI GAATGC 1 cut(s) 289
PfeI GAWTC 1 cut(s) 293
PkrI GCNGC 2 cut(s) 241, 349
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PspN4I GGNNCC 1 cut(s) 51
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
SaqAI TTAA 2 cut(s) 27, 120
SatI GCNGC 2 cut(s) 240, 348
Sau3AI GATC 4 cut(s) 49, 148, 230, 301
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 1 cut(s) 13
SetI ASST 5 cut(s) 21, 39, 79, 265, 349
SfcI CTRYAG 1 cut(s) 342
SphI GCATGC 1 cut(s) 341
Sse9I AATT 2 cut(s) 43, 143
SspMI CTAG 1 cut(s) 400
StyD4I CCNGG 1 cut(s) 154
TaiI ACGT 1 cut(s) 21
TaqI TCGA 1 cut(s) 151
TasI AATT 2 cut(s) 43, 143
TfiI GAWTC 1 cut(s) 293
Tru1I TTAA 2 cut(s) 27, 120
Tru9I TTAA 2 cut(s) 27, 120
TscAI CASTG 1 cut(s) 197
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 2 cut(s) 239, 347
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 2 cut(s) 160, 329
TspRI CASTG 1 cut(s) 197
XceI RCATGY 1 cut(s) 341
XspI CTAG 1 cut(s) 400
Zsp2I ATGCAT 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.