MD14G1220300.v1.1

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001969
Physical Location & Seq
Reverse (-)
30240003 .. 30241001
999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1220300.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGCAACCTCCAAAGCATTTCTCTTCTTTTTTCTTCTTTCTACATCCCAAGTCCTTTTTGTATCTGGTACCAAATTCGAAGTTGGAGGCAAGAGTGGATGGGAGGTCCCGAAATCGAAGAACGGCCAACAAATGTACAACGAATGGGCTTCGAAAAACCGGTTCAAGGTCGACGACGCTCTCTCTTTCAATTACACGAAAGACTCGGTGATGGTGGTGACCAAGGACGAGTACGAAAAATGCCACTCTGCCCATCCCATCTTCTACTCCAACAACGGTGCAACTACGTTCACACTGGACCGGCCTGGTTTGTTCTACTTCATCAGCGGAGTTGCCGGGCACTGCGAGAGAGGGCAGAAGATGATCATCAAGGTCTTGGAGCCGGCAACCCCGCCTCAATCTGCTGATCAGAACGCAACGGACCCGTCGCAGCCGAAGCATGGTTCGGCTGCAACGGCGGCCATCTCCTCTGCAGCAACTCTTGTGTTCTGCATGGTGTCATTTGTTGTGGTCTTTTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

175

Amino Acids

19.16

Weight (kDa)

8.4

Isoelectric Point (pI)

27.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 39 - 119 8e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 68
AccB1I GGYRCC 1 cut(s) 68
AccI GTMKAC 1 cut(s) 171
AciI CCGC 3 cut(s) 327, 392, 458
AcoI YGGCCR 2 cut(s) 124, 459
AcsI RAATTY 1 cut(s) 74
AfaI GTAC 3 cut(s) 70, 137, 233
AfiI CCNNNNNNNGG 2 cut(s) 166, 440
AgeI ACCGGT 1 cut(s) 159
AgsI TTSAA 2 cut(s) 166, 190
AjnI CCWGG 1 cut(s) 304
AoxI GGCC 3 cut(s) 124, 302, 459
ApeKI GCWGC 3 cut(s) 430, 449, 473
ApoI RAATTY 1 cut(s) 74
AsiGI ACCGGT 1 cut(s) 159
Asp718I GGTACC 1 cut(s) 68
AspS9I GGNCC 3 cut(s) 106, 298, 421
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 2 cut(s) 220, 229
AsuII TTCGAA 2 cut(s) 78, 152
AvaII GGWCC 3 cut(s) 106, 298, 421
BaeGI GKGCMC 1 cut(s) 342
BanI GGYRCC 1 cut(s) 68
BbvI GCAGC 3 cut(s) 436, 442, 485
BccI CCATC 5 cut(s) 93, 205, 261, 266, 470
BceAI ACGGC 2 cut(s) 139, 471
BciT130I CCWGG 1 cut(s) 306
BclI TGATCA 2 cut(s) 363, 406
BcnI CCSGG 1 cut(s) 337
BfaI CTAG 1 cut(s) 523
BfmI CTRYAG 1 cut(s) 471
BisI GCNGC 4 cut(s) 431, 450, 459, 474
BlsI GCNGC 4 cut(s) 432, 451, 460, 475
Bme1390I CCNGG 2 cut(s) 306, 337
Bme18I GGWCC 3 cut(s) 106, 298, 421
BmgT120I GGNCC 3 cut(s) 106, 298, 421
BmiI GGNNCC 4 cut(s) 70, 108, 381, 423
BmrFI CCNGG 2 cut(s) 306, 337
Bpu14I TTCGAA 2 cut(s) 78, 152
BpuMI CCSGG 1 cut(s) 337
BsaBI GATNNNNATC 1 cut(s) 365
BsaJI CCNNGG 1 cut(s) 222
BsaWI WCCGGW 1 cut(s) 159
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 440
Bse118I RCCGGY 3 cut(s) 159, 300, 382
Bse1I ACTGG 1 cut(s) 300
Bse8I GATNNNNATC 1 cut(s) 365
BseBI CCWGG 1 cut(s) 306
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 3 cut(s) 44, 104, 253
BseJI GATNNNNATC 1 cut(s) 365
BseLI CCNNNNNNNGG 2 cut(s) 166, 440
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 457
BseSI GKGCMC 1 cut(s) 342
BseXI GCAGC 3 cut(s) 436, 442, 485
BshFI GGCC 3 cut(s) 126, 304, 461
BshNI GGYRCC 1 cut(s) 68
BshTI ACCGGT 1 cut(s) 159
BsiSI CCGG 4 cut(s) 160, 301, 336, 383
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 2 cut(s) 166, 440
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 3 cut(s) 126, 304, 461
Bsp119I TTCGAA 2 cut(s) 78, 152
Bsp1286I GDGCHC 1 cut(s) 342
Bsp1407I TGTACA 1 cut(s) 135
Bsp143I GATC 2 cut(s) 363, 406
BspACI CCGC 3 cut(s) 327, 392, 458
BspANI GGCC 3 cut(s) 126, 304, 461
BspLI GGNNCC 4 cut(s) 70, 108, 381, 423
BspMAI CTGCAG 1 cut(s) 475
BspT104I TTCGAA 2 cut(s) 78, 152
BspT107I GGYRCC 1 cut(s) 68
BsrFI RCCGGY 3 cut(s) 159, 300, 382
BsrGI TGTACA 1 cut(s) 135
BsrI ACTGG 1 cut(s) 300
BssAI RCCGGY 3 cut(s) 159, 300, 382
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 2 cut(s) 363, 406
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 278
Bst6I CTCTTC 1 cut(s) 29
BstAUI TGTACA 1 cut(s) 135
BstBI TTCGAA 2 cut(s) 78, 152
BstC8I GCNNGC 1 cut(s) 384
BstEII GGTNACC 1 cut(s) 217
BstF5I GGATG 3 cut(s) 44, 104, 253
BstKTI GATC 2 cut(s) 366, 409
BstMBI GATC 2 cut(s) 363, 406
BstMWI GCNNNNNNNGC 3 cut(s) 436, 455, 458
BstNI CCWGG 1 cut(s) 306
BstPI GGTNACC 1 cut(s) 217
BstSCI CCNGG 2 cut(s) 304, 335
BstSFI CTRYAG 1 cut(s) 471
BstSLI GKGCMC 1 cut(s) 342
BstV1I GCAGC 3 cut(s) 436, 442, 485
BsuRI GGCC 3 cut(s) 126, 304, 461
BtsCI GGATG 3 cut(s) 44, 104, 253
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 2 cut(s) 293, 340
Cac8I GCNNGC 1 cut(s) 384
Cfr10I RCCGGY 3 cut(s) 159, 300, 382
Cfr13I GGNCC 3 cut(s) 106, 298, 421
CseI GACGC 1 cut(s) 185
Csp6I GTAC 3 cut(s) 69, 136, 232
CspAI ACCGGT 1 cut(s) 159
CviAII CATG 2 cut(s) 440, 493
CviJI RGCY 7 cut(s) 126, 149, 304, 382, 433, 449, 461
CviKI_1 RGCY 7 cut(s) 126, 149, 304, 382, 433, 449, 461
CviQI GTAC 3 cut(s) 69, 136, 232
DpnI GATC 2 cut(s) 365, 408
DpnII GATC 2 cut(s) 363, 406
EaeI YGGCCR 2 cut(s) 124, 459
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 222
Eco47I GGWCC 3 cut(s) 106, 298, 421
Eco91I GGTNACC 1 cut(s) 217
EcoO109I RGGNCCY 1 cut(s) 106
EcoO65I GGTNACC 1 cut(s) 217
EcoRII CCWGG 1 cut(s) 304
EcoT14I CCWWGG 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 2 cut(s) 443, 496
FaiI YATR 2 cut(s) 441, 494
FaqI GGGAC 1 cut(s) 92
FatI CATG 2 cut(s) 439, 492
FauI CCCGC 1 cut(s) 399
FbaI TGATCA 2 cut(s) 363, 406
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 4 cut(s) 431, 450, 459, 474
FokI GGATG 3 cut(s) 31, 111, 240
Fsp4HI GCNGC 4 cut(s) 431, 450, 459, 474
FspBI CTAG 1 cut(s) 523
GluI GCNGC 4 cut(s) 431, 450, 459, 474
HaeIII GGCC 3 cut(s) 126, 304, 461
HapII CCGG 4 cut(s) 160, 301, 336, 383
HgaI GACGC 1 cut(s) 185
Hin1II CATG 2 cut(s) 443, 496
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 1 cut(s) 203
HpaII CCGG 4 cut(s) 160, 301, 336, 383
HphI GGTGA 2 cut(s) 220, 229
Hpy166II GTNNAC 2 cut(s) 172, 291
Hpy188I TCNGA 1 cut(s) 411
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 2 cut(s) 172, 291
Hpy99I CGWCG 3 cut(s) 176, 179, 430
HpyCH4III ACNGT 1 cut(s) 278
HpyCH4IV ACGT 1 cut(s) 287
HpyCH4V TGCA 4 cut(s) 281, 452, 473, 492
HpyF10VI GCNNNNNNNGC 3 cut(s) 436, 455, 458
HpySE526I ACGT 1 cut(s) 287
Hsp92II CATG 2 cut(s) 443, 496
KpnI GGTACC 1 cut(s) 72
KroI GCCGGC 1 cut(s) 382
KroNI GCCGGC 1 cut(s) 384
Ksp22I TGATCA 2 cut(s) 363, 406
Kzo9I GATC 2 cut(s) 363, 406
LmnI GCTCC 1 cut(s) 379
LpnPI CCDG 8 cut(s) 51, 173, 281, 291, 314, 318, 349, 396
Lsp1109I GCAGC 3 cut(s) 436, 442, 485
MaeI CTAG 1 cut(s) 523
MaeII ACGT 1 cut(s) 287
MaeIII GTNAC 1 cut(s) 217
MalI GATC 2 cut(s) 365, 408
MboI GATC 2 cut(s) 363, 406
MboII GAAGA 6 cut(s) 16, 26, 130, 253, 370, 511
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 2 cut(s) 74, 190
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 2 cut(s) 64, 294
MnlI CCTC 6 cut(s) 19, 80, 97, 344, 405, 478
MroNI GCCGGC 1 cut(s) 382
MspA1I CMGCKG 1 cut(s) 327
MspI CCGG 4 cut(s) 160, 301, 336, 383
MspR9I CCNGG 2 cut(s) 306, 337
MvaI CCWGG 1 cut(s) 306
MwoI GCNNNNNNNGC 3 cut(s) 436, 455, 458
NaeI GCCGGC 1 cut(s) 384
NciI CCSGG 1 cut(s) 337
NdeII GATC 2 cut(s) 363, 406
NgoMIV GCCGGC 1 cut(s) 382
NlaIII CATG 2 cut(s) 443, 496
NlaIV GGNNCC 4 cut(s) 70, 108, 381, 423
NmuCI GTSAC 1 cut(s) 217
NspV TTCGAA 2 cut(s) 78, 152
PdiI GCCGGC 1 cut(s) 384
PinAI ACCGGT 1 cut(s) 159
PkrI GCNGC 4 cut(s) 432, 451, 460, 475
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
PpuMI RGGWCCY 1 cut(s) 106
Psp5II RGGWCCY 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 304
PspEI GGTNACC 1 cut(s) 217
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 4 cut(s) 70, 108, 381, 423
PspPI GGNCC 3 cut(s) 106, 298, 421
PspPPI RGGWCCY 1 cut(s) 106
PstI CTGCAG 1 cut(s) 475
RsaI GTAC 3 cut(s) 70, 137, 233
RsaNI GTAC 3 cut(s) 69, 136, 232
SalI GTCGAC 1 cut(s) 170
SatI GCNGC 4 cut(s) 431, 450, 459, 474
Sau3AI GATC 2 cut(s) 363, 406
Sau96I GGNCC 3 cut(s) 106, 298, 421
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 2 cut(s) 306, 337
SduI GDGCHC 1 cut(s) 342
SetI ASST 5 cut(s) 11, 108, 171, 290, 375
SfcI CTRYAG 1 cut(s) 471
SfuI TTCGAA 2 cut(s) 78, 152
SinI GGWCC 3 cut(s) 106, 298, 421
Sse9I AATT 2 cut(s) 74, 190
SsiI CCGC 3 cut(s) 327, 392, 458
SspMI CTAG 1 cut(s) 523
StyD4I CCNGG 2 cut(s) 304, 335
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 278
TaiI ACGT 1 cut(s) 290
TaqI TCGA 4 cut(s) 78, 116, 152, 171
TasI AATT 2 cut(s) 74, 190
TatI WGTACW 1 cut(s) 135
TauI GCSGC 1 cut(s) 461
TscAI CASTG 2 cut(s) 300, 347
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 3 cut(s) 430, 449, 473
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 310
TspGWI ACGGA 1 cut(s) 434
TspRI CASTG 2 cut(s) 300, 347
VpaK11BI GGWCC 3 cut(s) 106, 298, 421
XapI RAATTY 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.