MD14G1231000.v1.1

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001969
Physical Location & Seq
Forward (+)
31149425 .. 31150278
854 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1231000.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGACTCCTCCAAAGTATTTCTCTTATTTTTTCTTATTTCTAGTATCCAAGTCTTTTTTGTAACTTGTACTCAGTTTGAAGTTGAAGACAAGAGCAATGGATGGGAGGTACCCAAATCAAAAACTGACCAAGATATGTACAACAAATGGGCCTCCAAGAAAAGGTTCAAGGTTGATGACACTATCTACTTCAGTTACAAGAAAAACACGGACTCAGTGTTGGTAGTGACCAAGGAAGATTACAAAAATTGCAATTCGGATCAACCGATTTACTTCTCAAACGATGGTTACAGCGTGGTGATACTGGACCGACCCGGTTTGTTCTATTTCATCAGTGGGGTTGCTGGACGTTGTGAGAAAGGCCAGAAGATGATCATCAAAGTCATGGAACCAGTTGCTGTAGGTGCACCTAACCAATCTCCAAAAGACCACAGTGCAACTGACTCACCACCACAGCAGAACGCCTCCAAAAACCATAAGGCCAATGGTGCATTTGCAGTCTCTGCTGCCGTCTCTTCCACATCTATTATCTTATGCATCATAATATCACTCCTTGGGCTCTTTTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

191

Amino Acids

21.25

Weight (kDa)

8.26

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 43 - 122 3.1e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 109
AccB1I GGYRCC 1 cut(s) 109
AclWI GGATC 1 cut(s) 267
AcuI CTGAAG 1 cut(s) 175
AfaI GTAC 3 cut(s) 70, 111, 140
AfiI CCNNNNNNNGG 1 cut(s) 162
AgsI TTSAA 3 cut(s) 80, 86, 169
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 2 cut(s) 505, 517
Alw44I GTGCAC 1 cut(s) 405
AlwI GGATC 1 cut(s) 267
AlwNI CAGNNNCTG 2 cut(s) 398, 503
AoxI GGCC 3 cut(s) 150, 361, 480
ApaLI GTGCAC 1 cut(s) 405
ApeKI GCWGC 1 cut(s) 506
Asp700I GAANNNNTTC 1 cut(s) 164
Asp718I GGTACC 1 cut(s) 109
AspS9I GGNCC 2 cut(s) 150, 307
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 2 cut(s) 310, 438
AvaII GGWCC 1 cut(s) 307
BaeGI GKGCMC 1 cut(s) 409
BanI GGYRCC 1 cut(s) 109
BanII GRGCYC 1 cut(s) 561
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 409
BbvI GCAGC 1 cut(s) 493
BccI CCATC 2 cut(s) 96, 278
BceAI ACGGC 1 cut(s) 494
BciVI GTATCC 1 cut(s) 56
BclI TGATCA 1 cut(s) 372
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 2 cut(s) 505, 517
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 1 cut(s) 399
BfuI GTATCC 1 cut(s) 56
BisI GCNGC 1 cut(s) 507
BlsI GCNGC 1 cut(s) 508
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 307
BmgT120I GGNCC 2 cut(s) 150, 307
BmiI GGNNCC 2 cut(s) 111, 390
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 546
BpiI GAAGAC 1 cut(s) 93
BpuMI CCSGG 1 cut(s) 315
BsaBI GATNNNNATC 1 cut(s) 374
BsaJI CCNNGG 2 cut(s) 231, 553
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse1I ACTGG 2 cut(s) 309, 392
Bse3DI GCAATG 1 cut(s) 103
Bse8I GATNNNNATC 1 cut(s) 374
BseDI CCNNGG 2 cut(s) 231, 553
BseGI GGATG 1 cut(s) 107
BseJI GATNNNNATC 1 cut(s) 374
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 103
BseMII CTCAG 2 cut(s) 86, 228
BseNI ACTGG 2 cut(s) 309, 392
BseSI GKGCMC 1 cut(s) 409
BseXI GCAGC 1 cut(s) 493
BshFI GGCC 3 cut(s) 152, 363, 482
BshNI GGYRCC 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 409
BsiSI CCGG 1 cut(s) 315
BslI CCNNNNNNNGG 1 cut(s) 162
BsmAI GTCTC 2 cut(s) 505, 517
BsmBI CGTCTC 1 cut(s) 517
BsnI GGCC 3 cut(s) 152, 363, 482
Bsp1286I GDGCHC 2 cut(s) 409, 561
Bsp1407I TGTACA 1 cut(s) 138
Bsp143I GATC 2 cut(s) 259, 372
BspANI GGCC 3 cut(s) 152, 363, 482
BspCNI CTCAG 2 cut(s) 85, 227
BspLI GGNNCC 2 cut(s) 111, 390
BspPI GGATC 1 cut(s) 267
BspT107I GGYRCC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 103
BsrGI TGTACA 1 cut(s) 138
BsrI ACTGG 2 cut(s) 309, 392
BssECI CCNNGG 2 cut(s) 231, 553
BssMI GATC 2 cut(s) 259, 372
BssT1I CCWWGG 2 cut(s) 231, 553
Bst4CI ACNGT 1 cut(s) 434
Bst6I CTCTTC 1 cut(s) 520
BstAPI GCANNNNNTGC 1 cut(s) 503
BstAUI TGTACA 1 cut(s) 138
BstDEI CTNAG 2 cut(s) 72, 214
BstF5I GGATG 1 cut(s) 107
BstKTI GATC 2 cut(s) 262, 375
BstMAI GTCTC 2 cut(s) 505, 517
BstMBI GATC 2 cut(s) 259, 372
BstMWI GCNNNNNNNGC 3 cut(s) 404, 488, 503
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 399
BstSLI GKGCMC 1 cut(s) 409
BstV1I GCAGC 1 cut(s) 493
BstV2I GAAGAC 1 cut(s) 93
BsuI GTATCC 1 cut(s) 56
BsuRI GGCC 3 cut(s) 152, 363, 482
BtsCI GGATG 1 cut(s) 107
BtsIMutI CAGTG 3 cut(s) 222, 340, 439
CaiI CAGNNNCTG 2 cut(s) 398, 503
Cfr13I GGNCC 2 cut(s) 150, 307
Csp6I GTAC 3 cut(s) 69, 110, 139
CviAII CATG 1 cut(s) 385
CviJI RGCY 4 cut(s) 152, 363, 482, 559
CviKI_1 RGCY 4 cut(s) 152, 363, 482, 559
CviQI GTAC 3 cut(s) 69, 110, 139
DdeI CTNAG 2 cut(s) 72, 214
DpnI GATC 2 cut(s) 261, 374
DpnII GATC 2 cut(s) 259, 372
Eam1104I CTCTTC 1 cut(s) 520
EarI CTCTTC 1 cut(s) 520
Eco130I CCWWGG 2 cut(s) 231, 553
Eco24I GRGCYC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 175
EcoT14I CCWWGG 2 cut(s) 231, 553
EcoT22I ATGCAT 1 cut(s) 539
EcoT38I GRGCYC 1 cut(s) 561
ErhI CCWWGG 2 cut(s) 231, 553
Esp3I CGTCTC 1 cut(s) 517
FaeI CATG 1 cut(s) 388
FaiI YATR 5 cut(s) 137, 386, 477, 535, 542
FatI CATG 1 cut(s) 384
FbaI TGATCA 1 cut(s) 372
Fnu4HI GCNGC 1 cut(s) 507
FokI GGATG 1 cut(s) 114
FriOI GRGCYC 1 cut(s) 561
Fsp4HI GCNGC 1 cut(s) 507
FspBI CTAG 1 cut(s) 42
GluI GCNGC 1 cut(s) 507
HaeIII GGCC 3 cut(s) 152, 363, 482
HapII CCGG 1 cut(s) 315
Hin1II CATG 1 cut(s) 388
HinfI GANTC 3 cut(s) 5, 212, 443
HpaII CCGG 1 cut(s) 315
HphI GGTGA 2 cut(s) 310, 438
Hpy166II GTNNAC 1 cut(s) 407
Hpy188I TCNGA 1 cut(s) 259
Hpy8I GTNNAC 1 cut(s) 407
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 6 cut(s) 252, 407, 437, 491, 497, 537
HpyF10VI GCNNNNNNNGC 3 cut(s) 404, 488, 503
HpyF3I CTNAG 2 cut(s) 72, 214
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 1 cut(s) 388
KpnI GGTACC 1 cut(s) 113
Ksp22I TGATCA 1 cut(s) 372
Kzo9I GATC 2 cut(s) 259, 372
LpnPI CCDG 5 cut(s) 290, 328, 330, 377, 405
Lsp1109I GCAGC 1 cut(s) 493
LweI GCATC 1 cut(s) 546
MaeI CTAG 1 cut(s) 42
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 4 cut(s) 61, 194, 226, 287
MalI GATC 2 cut(s) 261, 374
MboI GATC 2 cut(s) 259, 372
MboII GAAGA 5 cut(s) 98, 248, 379, 507, 559
MhlI GDGCHC 2 cut(s) 409, 561
MluCI AATT 2 cut(s) 247, 253
MlyI GAGTC 2 cut(s) 206, 437
MnlI CCTC 4 cut(s) 19, 100, 163, 475
Mph1103I ATGCAT 1 cut(s) 539
MroXI GAANNNNTTC 1 cut(s) 164
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 1 cut(s) 315
MwoI GCNNNNNNNGC 3 cut(s) 404, 488, 503
NciI CCSGG 1 cut(s) 315
NdeII GATC 2 cut(s) 259, 372
NlaIII CATG 1 cut(s) 388
NlaIV GGNNCC 2 cut(s) 111, 390
NmuCI GTSAC 1 cut(s) 226
NsiI ATGCAT 1 cut(s) 539
PcsI WCGNNNNNNNCGW 1 cut(s) 263
PdmI GAANNNNTTC 1 cut(s) 164
PkrI GCNGC 1 cut(s) 508
PleI GAGTC 2 cut(s) 206, 437
PpsI GAGTC 2 cut(s) 206, 437
PspN4I GGNNCC 2 cut(s) 111, 390
PspPI GGNCC 2 cut(s) 150, 307
PstNI CAGNNNCTG 2 cut(s) 398, 503
RsaI GTAC 3 cut(s) 70, 111, 140
RsaNI GTAC 3 cut(s) 69, 110, 139
SatI GCNGC 1 cut(s) 507
Sau3AI GATC 2 cut(s) 259, 372
Sau96I GGNCC 2 cut(s) 150, 307
SchI GAGTC 2 cut(s) 206, 437
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 2 cut(s) 409, 561
SetI ASST 6 cut(s) 111, 167, 174, 352, 406, 412
SfaNI GCATC 1 cut(s) 546
SfcI CTRYAG 1 cut(s) 399
SinI GGWCC 1 cut(s) 307
Sse9I AATT 2 cut(s) 247, 253
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 2 cut(s) 231, 553
TaaI ACNGT 1 cut(s) 434
TaiI ACGT 1 cut(s) 352
TaqII GACCGA 1 cut(s) 324
TasI AATT 2 cut(s) 247, 253
TatI WGTACW 2 cut(s) 68, 138
TscAI CASTG 3 cut(s) 222, 340, 439
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 1 cut(s) 506
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 319
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 3 cut(s) 222, 340, 439
VneI GTGCAC 1 cut(s) 405
VpaK11BI GGWCC 1 cut(s) 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XmnI GAANNNNTTC 1 cut(s) 164
XspI CTAG 1 cut(s) 42
Zsp2I ATGCAT 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.