RchiOBHm_Chr7g0184371

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001969
Physical Location & Seq
Reverse (-)
4956715 .. 4957670
956 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16449

Sequence Viewer

Length: 501 bp
ATGGAAACCTCCAAGGTGTTTGTCTTGCTTTTTCTTCTTTCCACCTTCCATTTCCTTGTTGCGACTTGCATCCAGTTTGAAGTTGGAGGGAAGAGTGGATGGGAGGTCCCTAAATCAAAGGATGACTACAACCAATGGGCATCAACAAACCGGTTCAAAGTAAACGACACTGTTCATTTCAGCTACAAGGAAGACTCGGTGATGGTGGTAACGGAGGACGAGTATGACAAGTGCCATTCGGCACGTCCGATCTTCTTCAACAACAATGGAAACACTGAGTACAAGTTGGCCCGGCCGGGGTTGTTCTACTTCATAAGTGGGGTGAAAGGGCACTGTCCGAGAGGCCAGAAGATGATCATCAAGGTGTTGGAACCGGCGAGCCCTCCTCAGCCTTCTATAGACTCGCAGAAAAAGAATGGTGCTGCTGCAATGGCTGCTGCTGTTTCTTCTGCAACTGTTATGCTTTGCGTTGTGTCATTCCTTGCGGTTTTCCTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

166

Amino Acids

18.51

Weight (kDa)

8.32

Isoelectric Point (pI)

27.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 34 - 116 3.1e-20 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 485
AcoI YGGCCR 1 cut(s) 293
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 297
AgeI ACCGGT 1 cut(s) 150
AgsI TTSAA 3 cut(s) 80, 157, 259
AjiI CACGTC 1 cut(s) 245
AluBI AGCT 1 cut(s) 183
AluI AGCT 1 cut(s) 183
AoxI GGCC 3 cut(s) 288, 293, 343
ApeKI GCWGC 4 cut(s) 422, 425, 434, 437
AsiGI ACCGGT 1 cut(s) 150
AspS9I GGNCC 2 cut(s) 106, 289
AsuC2I CCSGG 2 cut(s) 292, 297
AsuHPI GGTGA 2 cut(s) 211, 334
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 333
BanII GRGCYC 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 198
BbvCI CCTCAGC 1 cut(s) 387
BbvI GCAGC 4 cut(s) 409, 412, 421, 424
BccI CCATC 2 cut(s) 93, 196
BclI TGATCA 1 cut(s) 354
BcnI CCSGG 2 cut(s) 292, 297
BfmI CTRYAG 1 cut(s) 396
BisI GCNGC 4 cut(s) 423, 426, 435, 438
BlsI GCNGC 4 cut(s) 424, 427, 436, 439
Bme1390I CCNGG 2 cut(s) 292, 297
Bme18I GGWCC 1 cut(s) 106
BmgBI CACGTC 1 cut(s) 245
BmgT120I GGNCC 2 cut(s) 106, 289
BmiI GGNNCC 2 cut(s) 108, 372
BmrFI CCNGG 2 cut(s) 292, 297
BmsI GCATC 2 cut(s) 78, 149
BpiI GAAGAC 1 cut(s) 198
BplI GAGNNNNNCTC 2 cut(s) 370, 402
Bpu10I CCTNAGC 1 cut(s) 387
BpuMI CCSGG 2 cut(s) 292, 297
BsaBI GATNNNNATC 1 cut(s) 356
BsaJI CCNNGG 2 cut(s) 12, 296
BsaWI WCCGGW 1 cut(s) 150
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 1 cut(s) 297
Bse118I RCCGGY 2 cut(s) 150, 373
Bse1I ACTGG 1 cut(s) 73
Bse3DI GCAATG 1 cut(s) 435
Bse8I GATNNNNATC 1 cut(s) 356
BseDI CCNNGG 2 cut(s) 12, 296
BseGI GGATG 3 cut(s) 69, 104, 127
BseJI GATNNNNATC 1 cut(s) 356
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 2 cut(s) 267, 401
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 375
BseSI GKGCMC 1 cut(s) 333
BseX3I CGGCCG 1 cut(s) 293
BseXI GCAGC 4 cut(s) 409, 412, 421, 424
Bsh1285I CGRYCG 1 cut(s) 296
BshFI GGCC 3 cut(s) 290, 295, 345
BshTI ACCGGT 1 cut(s) 150
BsiEI CGRYCG 1 cut(s) 296
BsiSI CCGG 4 cut(s) 151, 292, 296, 374
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 297
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 3 cut(s) 290, 295, 345
Bsp1286I GDGCHC 2 cut(s) 333, 383
Bsp143I GATC 2 cut(s) 249, 354
BspACI CCGC 1 cut(s) 485
BspANI GGCC 3 cut(s) 290, 295, 345
BspCNI CTCAG 2 cut(s) 268, 400
BspLI GGNNCC 2 cut(s) 108, 372
BsrDI GCAATG 1 cut(s) 435
BsrFI RCCGGY 2 cut(s) 150, 373
BsrI ACTGG 1 cut(s) 73
BssAI RCCGGY 2 cut(s) 150, 373
BssECI CCNNGG 2 cut(s) 12, 296
BssMI GATC 2 cut(s) 249, 354
BssT1I CCWWGG 1 cut(s) 12
Bst4CI ACNGT 3 cut(s) 172, 335, 457
Bst6I CTCTTC 1 cut(s) 86
BstAPI GCANNNNNTGC 1 cut(s) 434
BstC8I GCNNGC 1 cut(s) 379
BstDEI CTNAG 2 cut(s) 276, 387
BstF5I GGATG 3 cut(s) 69, 104, 127
BstKTI GATC 2 cut(s) 252, 357
BstMBI GATC 2 cut(s) 249, 354
BstMCI CGRYCG 1 cut(s) 296
BstMWI GCNNNNNNNGC 2 cut(s) 431, 434
BstSCI CCNGG 2 cut(s) 290, 295
BstSFI CTRYAG 1 cut(s) 396
BstSLI GKGCMC 1 cut(s) 333
BstV1I GCAGC 4 cut(s) 409, 412, 421, 424
BstV2I GAAGAC 1 cut(s) 198
BstZI CGGCCG 1 cut(s) 293
BsuRI GGCC 3 cut(s) 290, 295, 345
BtrI CACGTC 1 cut(s) 245
BtsCI GGATG 3 cut(s) 69, 104, 127
BtsIMutI CAGTG 3 cut(s) 168, 273, 331
Cac8I GCNNGC 1 cut(s) 379
Cfr10I RCCGGY 2 cut(s) 150, 373
Cfr13I GGNCC 2 cut(s) 106, 289
Csp6I GTAC 1 cut(s) 280
CspAI ACCGGT 1 cut(s) 150
CviJI RGCY 7 cut(s) 183, 290, 295, 345, 381, 391, 434
CviKI_1 RGCY 7 cut(s) 183, 290, 295, 345, 381, 391, 434
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 2 cut(s) 276, 387
DpnI GATC 2 cut(s) 251, 356
DpnII GATC 2 cut(s) 249, 354
EaeI YGGCCR 1 cut(s) 293
EagI CGGCCG 1 cut(s) 293
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
EclXI CGGCCG 1 cut(s) 293
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 106
Eco52I CGGCCG 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 383
ErhI CCWWGG 1 cut(s) 12
FaiI YATR 4 cut(s) 225, 314, 398, 461
FaqI GGGAC 1 cut(s) 92
FbaI TGATCA 1 cut(s) 354
Fnu4HI GCNGC 4 cut(s) 423, 426, 435, 438
FokI GGATG 3 cut(s) 56, 111, 134
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 4 cut(s) 423, 426, 435, 438
GluI GCNGC 4 cut(s) 423, 426, 435, 438
HaeIII GGCC 3 cut(s) 290, 295, 345
HapII CCGG 4 cut(s) 151, 292, 296, 374
HinfI GANTC 2 cut(s) 194, 401
HpaII CCGG 4 cut(s) 151, 292, 296, 374
HphI GGTGA 2 cut(s) 211, 334
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 2 cut(s) 249, 339
Hpy8I GTNNAC 1 cut(s) 163
HpyAV CCTTC 2 cut(s) 55, 402
HpyCH4III ACNGT 3 cut(s) 172, 335, 457
HpyCH4IV ACGT 1 cut(s) 244
HpyCH4V TGCA 3 cut(s) 69, 428, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 431, 434
HpyF3I CTNAG 2 cut(s) 276, 387
HpySE526I ACGT 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 2 cut(s) 249, 354
LpnPI CCDG 6 cut(s) 86, 164, 305, 309, 359, 387
Lsp1109I GCAGC 4 cut(s) 409, 412, 421, 424
LweI GCATC 2 cut(s) 78, 149
MaeII ACGT 1 cut(s) 244
MaeIII GTNAC 1 cut(s) 208
MalI GATC 2 cut(s) 251, 356
MboI GATC 2 cut(s) 249, 354
MboII GAAGA 7 cut(s) 26, 103, 203, 244, 247, 361, 438
MhlI GDGCHC 2 cut(s) 333, 383
MlyI GAGTC 2 cut(s) 188, 395
MmeI TCCRAC 2 cut(s) 64, 348
MnlI CCTC 7 cut(s) 19, 80, 97, 208, 335, 393, 396
MslI CAYNNNNRTG 1 cut(s) 362
MspI CCGG 4 cut(s) 151, 292, 296, 374
MspR9I CCNGG 2 cut(s) 292, 297
MwoI GCNNNNNNNGC 2 cut(s) 431, 434
NciI CCSGG 2 cut(s) 292, 297
NdeII GATC 2 cut(s) 249, 354
NlaIV GGNNCC 2 cut(s) 108, 372
PcsI WCGNNNNNNNCGW 1 cut(s) 245
PinAI ACCGGT 1 cut(s) 150
PkrI GCNGC 4 cut(s) 424, 427, 436, 439
PleI GAGTC 2 cut(s) 188, 395
PpsI GAGTC 2 cut(s) 188, 395
PpuMI RGGWCCY 1 cut(s) 106
Psp5II RGGWCCY 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 108, 372
PspPI GGNCC 2 cut(s) 106, 289
PspPPI RGGWCCY 1 cut(s) 106
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 1 cut(s) 362
SatI GCNGC 4 cut(s) 423, 426, 435, 438
Sau3AI GATC 2 cut(s) 249, 354
Sau96I GGNCC 2 cut(s) 106, 289
SchI GAGTC 2 cut(s) 188, 395
ScrFI CCNGG 2 cut(s) 292, 297
SduI GDGCHC 2 cut(s) 333, 383
SetI ASST 7 cut(s) 11, 18, 47, 108, 185, 247, 366
SfaNI GCATC 2 cut(s) 78, 149
SfcI CTRYAG 1 cut(s) 396
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 362
SsiI CCGC 1 cut(s) 485
StyD4I CCNGG 2 cut(s) 290, 295
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 3 cut(s) 172, 335, 457
TaiI ACGT 1 cut(s) 247
TatI WGTACW 1 cut(s) 279
TscAI CASTG 3 cut(s) 175, 280, 338
TseI GCWGC 4 cut(s) 422, 425, 434, 437
TspDTI ATGAA 2 cut(s) 164, 301
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 3 cut(s) 175, 280, 338
VpaK11BI GGWCC 1 cut(s) 106
XcmI CCANNNNNNNNNTGG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.