MD06G1209600.v1.1

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001969
Physical Location & Seq
Reverse (-)
34289136 .. 34289920
785 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1209600.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGAAACCTCCAAAGTATTTCTCTTCTTTCTTCTTCTTCTTTCTACCTTCCAAGTCCTTTTTGTATCTTGTACCAAATTTGAAGTTGGAGGCAAGAGTGGATGGGAGGTCCCGAAATCGAAGAGCCAACAAATGTACAACGAATGGGCGTCGAAAAACCGGTTCAAGGTCGACGACACTCTATCTTTCAATTACACGAAGGACTCGGTGGTGGTGGTGACCAAGGACGAGTACGAAAAATGCCACTCTGCTCATCCCATCTTCTTCTCCAACAATGGTGCAACTACGTTCACACTGGACCGGCCCGGTTTGTTCTACTTCATCAGCGGAGTTGCCGGGCACTGCGAGAGAGGGCAGAAGATGATCATCAAGGTCTTGGAACCGGCAAGCCCGCCTAAGTCTGCTGATCAGAACTCGACGGACCCGTCGCAGACGAAGCATGGTTCGGGTGCAATGGCCGCCATCTCCTCCGCAACAACTCTTGTGTTCTGCTTTGTGTCATTTGTTGTGGTCTTTTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

175

Amino Acids

19.39

Weight (kDa)

8.32

Isoelectric Point (pI)

25.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 35 - 119 4.7e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 424
AccI GTMKAC 1 cut(s) 171
AciI CCGC 4 cut(s) 327, 392, 459, 471
AcoI YGGCCR 1 cut(s) 456
AcsI RAATTY 1 cut(s) 77
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 3 cut(s) 73, 137, 233
AfiI CCNNNNNNNGG 1 cut(s) 166
AgeI ACCGGT 1 cut(s) 159
AgsI TTSAA 3 cut(s) 83, 166, 190
AoxI GGCC 2 cut(s) 302, 456
ApoI RAATTY 1 cut(s) 77
AsiGI ACCGGT 1 cut(s) 159
AspS9I GGNCC 4 cut(s) 109, 298, 303, 421
AsuC2I CCSGG 2 cut(s) 306, 337
AsuHPI GGTGA 1 cut(s) 229
AvaII GGWCC 3 cut(s) 109, 298, 421
BaeGI GKGCMC 1 cut(s) 342
BccI CCATC 3 cut(s) 96, 266, 470
BclI TGATCA 2 cut(s) 363, 406
BcnI CCSGG 2 cut(s) 306, 337
BfaI CTAG 1 cut(s) 523
BisI GCNGC 1 cut(s) 459
BlsI GCNGC 1 cut(s) 460
Bme1390I CCNGG 2 cut(s) 306, 337
Bme18I GGWCC 3 cut(s) 109, 298, 421
BmgT120I GGNCC 4 cut(s) 109, 298, 303, 421
BmiI GGNNCC 3 cut(s) 111, 381, 423
BmrFI CCNGG 2 cut(s) 306, 337
BpuMI CCSGG 2 cut(s) 306, 337
BsaBI GATNNNNATC 1 cut(s) 365
BsaHI GRCGYC 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 222
BsaWI WCCGGW 1 cut(s) 159
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse118I RCCGGY 3 cut(s) 159, 300, 382
Bse1I ACTGG 1 cut(s) 300
Bse3DI GCAATG 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 365
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 2 cut(s) 107, 253
BseJI GATNNNNATC 1 cut(s) 365
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMI GCAATG 1 cut(s) 459
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 457
BseSI GKGCMC 1 cut(s) 342
BshFI GGCC 2 cut(s) 304, 458
BshTI ACCGGT 1 cut(s) 159
BsiSI CCGG 5 cut(s) 160, 301, 306, 336, 383
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 2 cut(s) 304, 458
Bsp1286I GDGCHC 1 cut(s) 342
Bsp1407I TGTACA 1 cut(s) 135
Bsp143I GATC 2 cut(s) 363, 406
BspACI CCGC 4 cut(s) 327, 392, 459, 471
BspANI GGCC 2 cut(s) 304, 458
BspLI GGNNCC 3 cut(s) 111, 381, 423
BspQI GCTCTTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 459
BsrFI RCCGGY 3 cut(s) 159, 300, 382
BsrGI TGTACA 1 cut(s) 135
BsrI ACTGG 1 cut(s) 300
BssAI RCCGGY 3 cut(s) 159, 300, 382
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 2 cut(s) 363, 406
BssNI GRCGYC 1 cut(s) 149
BssT1I CCWWGG 1 cut(s) 222
Bst6I CTCTTC 2 cut(s) 29, 116
BstACI GRCGYC 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 135
BstC8I GCNNGC 2 cut(s) 388, 392
BstDEI CTNAG 1 cut(s) 396
BstEII GGTNACC 1 cut(s) 217
BstF5I GGATG 2 cut(s) 107, 253
BstKTI GATC 2 cut(s) 366, 409
BstMBI GATC 2 cut(s) 363, 406
BstMWI GCNNNNNNNGC 2 cut(s) 436, 458
BstPI GGTNACC 1 cut(s) 217
BstSCI CCNGG 2 cut(s) 304, 335
BstSLI GKGCMC 1 cut(s) 342
BsuRI GGCC 2 cut(s) 304, 458
BtsCI GGATG 2 cut(s) 107, 253
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 2 cut(s) 293, 340
Cac8I GCNNGC 2 cut(s) 388, 392
Cfr10I RCCGGY 3 cut(s) 159, 300, 382
Cfr13I GGNCC 4 cut(s) 109, 298, 303, 421
CseI GACGC 1 cut(s) 138
Csp6I GTAC 3 cut(s) 72, 136, 232
CspAI ACCGGT 1 cut(s) 159
CviAII CATG 1 cut(s) 440
CviJI RGCY 4 cut(s) 126, 304, 390, 458
CviKI_1 RGCY 4 cut(s) 126, 304, 390, 458
CviQI GTAC 3 cut(s) 72, 136, 232
DdeI CTNAG 1 cut(s) 396
DpnI GATC 2 cut(s) 365, 408
DpnII GATC 2 cut(s) 363, 406
DrdI GACNNNNNNGTC 1 cut(s) 424
DseDI GACNNNNNNGTC 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 456
Eam1104I CTCTTC 2 cut(s) 29, 116
EarI CTCTTC 2 cut(s) 29, 116
Eco130I CCWWGG 1 cut(s) 222
Eco47I GGWCC 3 cut(s) 109, 298, 421
Eco91I GGTNACC 1 cut(s) 217
EcoO109I RGGNCCY 1 cut(s) 109
EcoO65I GGTNACC 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 1 cut(s) 443
FaiI YATR 1 cut(s) 441
FaqI GGGAC 1 cut(s) 95
FatI CATG 1 cut(s) 439
FauI CCCGC 1 cut(s) 399
FbaI TGATCA 2 cut(s) 363, 406
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 459
FokI GGATG 2 cut(s) 114, 240
Fsp4HI GCNGC 1 cut(s) 459
FspBI CTAG 1 cut(s) 523
GluI GCNGC 1 cut(s) 459
HaeIII GGCC 2 cut(s) 304, 458
HapII CCGG 5 cut(s) 160, 301, 306, 336, 383
HgaI GACGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 1 cut(s) 443
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 1 cut(s) 203
HpaII CCGG 5 cut(s) 160, 301, 306, 336, 383
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 2 cut(s) 172, 291
Hpy188I TCNGA 1 cut(s) 411
Hpy188III TCNNGA 1 cut(s) 112
Hpy8I GTNNAC 2 cut(s) 172, 291
Hpy99I CGWCG 4 cut(s) 154, 176, 421, 430
HpyAV CCTTC 2 cut(s) 58, 193
HpyCH4IV ACGT 1 cut(s) 287
HpyCH4V TGCA 2 cut(s) 281, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 436, 458
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 1 cut(s) 287
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 1 cut(s) 443
Ksp22I TGATCA 2 cut(s) 363, 406
Kzo9I GATC 2 cut(s) 363, 406
LguI GCTCTTC 1 cut(s) 116
LpnPI CCDG 6 cut(s) 173, 281, 314, 319, 349, 396
MaeI CTAG 1 cut(s) 523
MaeII ACGT 1 cut(s) 287
MaeIII GTNAC 1 cut(s) 217
MalI GATC 2 cut(s) 365, 408
MboI GATC 2 cut(s) 363, 406
MboII GAAGA 9 cut(s) 16, 23, 26, 29, 133, 253, 256, 370, 511
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 2 cut(s) 77, 190
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 2 cut(s) 67, 294
MnlI CCTC 5 cut(s) 19, 83, 100, 344, 478
MspA1I CMGCKG 1 cut(s) 327
MspI CCGG 5 cut(s) 160, 301, 306, 336, 383
MspR9I CCNGG 2 cut(s) 306, 337
MwoI GCNNNNNNNGC 2 cut(s) 436, 458
NciI CCSGG 2 cut(s) 306, 337
NdeII GATC 2 cut(s) 363, 406
NlaIII CATG 1 cut(s) 443
NlaIV GGNNCC 3 cut(s) 111, 381, 423
NmuCI GTSAC 1 cut(s) 217
PciSI GCTCTTC 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 422
PinAI ACCGGT 1 cut(s) 159
PkrI GCNGC 1 cut(s) 460
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
PpuMI RGGWCCY 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 109
PspEI GGTNACC 1 cut(s) 217
PspN4I GGNNCC 3 cut(s) 111, 381, 423
PspPI GGNCC 4 cut(s) 109, 298, 303, 421
PspPPI RGGWCCY 1 cut(s) 109
RsaI GTAC 3 cut(s) 73, 137, 233
RsaNI GTAC 3 cut(s) 72, 136, 232
SalI GTCGAC 1 cut(s) 170
SapI GCTCTTC 1 cut(s) 116
SatI GCNGC 1 cut(s) 459
Sau3AI GATC 2 cut(s) 363, 406
Sau96I GGNCC 4 cut(s) 109, 298, 303, 421
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 2 cut(s) 306, 337
SduI GDGCHC 1 cut(s) 342
SetI ASST 6 cut(s) 11, 50, 111, 171, 290, 375
SinI GGWCC 3 cut(s) 109, 298, 421
Sse9I AATT 2 cut(s) 77, 190
SsiI CCGC 4 cut(s) 327, 392, 459, 471
SspMI CTAG 1 cut(s) 523
StyD4I CCNGG 2 cut(s) 304, 335
StyI CCWWGG 1 cut(s) 222
TaiI ACGT 1 cut(s) 290
TaqI TCGA 4 cut(s) 119, 152, 171, 416
TasI AATT 2 cut(s) 77, 190
TatI WGTACW 1 cut(s) 135
TauI GCSGC 1 cut(s) 461
TscAI CASTG 2 cut(s) 300, 347
TseFI GTSAC 1 cut(s) 217
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 310
TspGWI ACGGA 1 cut(s) 434
TspRI CASTG 2 cut(s) 300, 347
VpaK11BI GGWCC 3 cut(s) 109, 298, 421
XapI RAATTY 1 cut(s) 77
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.