FvH4_5g16790

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001969
Physical Location & Seq
Forward (+)
9589224 .. 9590765
1542 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16790.t1

Sequence Viewer

Length: 585 bp
ATGGAGTCCTCCAAAGTATTTCTCTTGTTCTTCTTTCTCTCTGTTATTCAGGTCGTCTTTGTAACCAGTACTGAGTTTCAAGTCGGTGACAAGAGCAATGGGTGGGAGGTCCCCAAATCAAAGAGTGACCAAGATATGTACAACGAGTGGGCTTCCAAGAACAGGTTCAAAGTTAATGACACTCTCAATTTCAATTTCAAGAAAGACGCAGACTCGGTTATGGTGGTGACAGAAGCCGAGTACGAAAAATGCCATTCGGATAAACCGATTTTCACCGCTAAAGATGGTGGCTCAGTGTTCAAATTGGATCGACCCGGTTTGTTCTACTTCATTAGTGGGACTGATGGCCACTGCGAGAAAGGGCAGAAGATGATCGTCAAAGTGTTGGCATCACCAGCTGCTGCTGAAACAGAAAGCCCACCACCTTCTCAGTCAGCAAACCAGAATGCAACTGCTGAAACACCAGCCTCTCATGATCATGCGAATCATGTTGACAAGAAGAACAATGCAGTTGCAATGCATGCTGCAGCTGCCATCACTCTTACAACTGCTGTAATGTCATTTCTTGGGGCCCTTTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

195

Amino Acids

21.31

Weight (kDa)

5.85

Isoelectric Point (pI)

28.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 40 - 122 7.2e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 276
AclWI GGATC 1 cut(s) 315
AcoI YGGCCR 1 cut(s) 346
AfaI GTAC 3 cut(s) 70, 140, 242
AfiI CCNNNNNNNGG 1 cut(s) 162
AgsI TTSAA 5 cut(s) 80, 169, 193, 199, 301
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 2 cut(s) 398, 530
AluI AGCT 2 cut(s) 398, 530
AlwI GGATC 1 cut(s) 315
AlwNI CAGNNNCTG 1 cut(s) 401
AoxI GGCC 2 cut(s) 346, 570
ApaI GGGCCC 1 cut(s) 574
ApeKI GCWGC 5 cut(s) 398, 401, 524, 527, 530
Asp700I GAANNNNTTC 1 cut(s) 164
AspS9I GGNCC 3 cut(s) 109, 570, 571
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 4 cut(s) 98, 238, 265, 384
AvaII GGWCC 1 cut(s) 109
BaeGI GKGCMC 1 cut(s) 574
BalI TGGCCA 1 cut(s) 348
BanII GRGCYC 1 cut(s) 574
BbvI GCAGC 5 cut(s) 385, 388, 511, 517, 539
BccI CCATC 3 cut(s) 278, 338, 542
BclI TGATCA 1 cut(s) 475
BcnI CCSGG 1 cut(s) 315
BfaI CTAG 1 cut(s) 583
BfmI CTRYAG 1 cut(s) 525
BisI GCNGC 5 cut(s) 399, 402, 525, 528, 531
BlsI GCNGC 5 cut(s) 400, 403, 526, 529, 532
BmcAI AGTACT 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 3 cut(s) 109, 570, 571
BmiI GGNNCC 3 cut(s) 111, 571, 572
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 398
BpuMI CCSGG 1 cut(s) 315
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse1I ACTGG 1 cut(s) 66
Bse3DI GCAATG 2 cut(s) 103, 522
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMI GCAATG 2 cut(s) 103, 522
BseMII CTCAG 3 cut(s) 63, 306, 443
BseNI ACTGG 1 cut(s) 66
BseSI GKGCMC 1 cut(s) 574
BseXI GCAGC 5 cut(s) 385, 388, 511, 517, 539
BshFI GGCC 2 cut(s) 348, 572
BsiSI CCGG 1 cut(s) 315
BslFI GGGAC 2 cut(s) 95, 352
BslI CCNNNNNNNGG 1 cut(s) 162
BsmFI GGGAC 2 cut(s) 95, 352
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 2 cut(s) 348, 572
Bsp120I GGGCCC 1 cut(s) 570
Bsp1286I GDGCHC 1 cut(s) 574
Bsp1407I TGTACA 1 cut(s) 138
Bsp143I GATC 3 cut(s) 307, 372, 475
BspACI CCGC 1 cut(s) 276
BspANI GGCC 2 cut(s) 348, 572
BspCNI CTCAG 3 cut(s) 64, 305, 442
BspHI TCATGA 1 cut(s) 472
BspLI GGNNCC 3 cut(s) 111, 571, 572
BspMAI CTGCAG 1 cut(s) 529
BspPI GGATC 1 cut(s) 315
BsrDI GCAATG 2 cut(s) 103, 522
BsrGI TGTACA 1 cut(s) 138
BsrI ACTGG 1 cut(s) 66
BssMI GATC 3 cut(s) 307, 372, 475
BstAPI GCANNNNNTGC 1 cut(s) 521
BstAUI TGTACA 1 cut(s) 138
BstC8I GCNNGC 1 cut(s) 522
BstDEI CTNAG 3 cut(s) 72, 292, 429
BstKTI GATC 3 cut(s) 310, 375, 478
BstMBI GATC 3 cut(s) 307, 372, 475
BstMWI GCNNNNNNNGC 3 cut(s) 395, 521, 530
BstNSI RCATGY 1 cut(s) 524
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 525
BstSLI GKGCMC 1 cut(s) 574
BstV1I GCAGC 5 cut(s) 385, 388, 511, 517, 539
BsuRI GGCC 2 cut(s) 348, 572
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 300, 349
Cac8I GCNNGC 1 cut(s) 522
CaiI CAGNNNCTG 1 cut(s) 401
CciI TCATGA 1 cut(s) 472
Cfr13I GGNCC 3 cut(s) 109, 570, 571
CseI GACGC 1 cut(s) 215
Csp6I GTAC 3 cut(s) 69, 139, 241
CviAII CATG 4 cut(s) 473, 479, 488, 521
CviJI RGCY 9 cut(s) 152, 236, 291, 348, 398, 417, 467, 530, 572
CviKI_1 RGCY 9 cut(s) 152, 236, 291, 348, 398, 417, 467, 530, 572
CviQI GTAC 3 cut(s) 69, 139, 241
DdeI CTNAG 3 cut(s) 72, 292, 429
DpnI GATC 3 cut(s) 309, 374, 477
DpnII GATC 3 cut(s) 307, 372, 475
EaeI YGGCCR 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 109
EcoO109I RGGNCCY 3 cut(s) 109, 570, 571
EcoT22I ATGCAT 1 cut(s) 522
EcoT38I GRGCYC 1 cut(s) 574
FaeI CATG 4 cut(s) 476, 482, 491, 524
FaiI YATR 6 cut(s) 137, 221, 474, 480, 489, 522
FaqI GGGAC 2 cut(s) 95, 352
FatI CATG 4 cut(s) 472, 478, 487, 520
FbaI TGATCA 1 cut(s) 475
Fnu4HI GCNGC 5 cut(s) 399, 402, 525, 528, 531
FriOI GRGCYC 1 cut(s) 574
Fsp4HI GCNGC 5 cut(s) 399, 402, 525, 528, 531
FspBI CTAG 1 cut(s) 583
GluI GCNGC 5 cut(s) 399, 402, 525, 528, 531
HaeIII GGCC 2 cut(s) 348, 572
HapII CCGG 1 cut(s) 315
HgaI GACGC 1 cut(s) 215
Hin1II CATG 4 cut(s) 476, 482, 491, 524
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HinfI GANTC 3 cut(s) 5, 212, 484
HpaII CCGG 1 cut(s) 315
HphI GGTGA 4 cut(s) 98, 238, 265, 384
Hpy166II GTNNAC 1 cut(s) 493
Hpy188I TCNGA 1 cut(s) 259
Hpy188III TCNNGA 2 cut(s) 199, 473
Hpy8I GTNNAC 1 cut(s) 493
HpyAV CCTTC 1 cut(s) 435
HpyCH4V TGCA 5 cut(s) 449, 509, 515, 520, 527
HpyF10VI GCNNNNNNNGC 3 cut(s) 395, 521, 530
HpyF3I CTNAG 3 cut(s) 72, 292, 429
Hsp92II CATG 4 cut(s) 476, 482, 491, 524
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 3 cut(s) 307, 372, 475
LpnPI CCDG 7 cut(s) 35, 79, 148, 328, 408, 455, 477
Lsp1109I GCAGC 5 cut(s) 385, 388, 511, 517, 539
LweI GCATC 1 cut(s) 398
MaeI CTAG 1 cut(s) 583
MaeIII GTNAC 4 cut(s) 61, 86, 125, 226
MalI GATC 3 cut(s) 309, 374, 477
MboI GATC 3 cut(s) 307, 372, 475
MboII GAAGA 4 cut(s) 22, 379, 511, 571
MhlI GDGCHC 1 cut(s) 574
MlsI TGGCCA 1 cut(s) 348
MluCI AATT 3 cut(s) 187, 193, 302
MluNI TGGCCA 1 cut(s) 348
MlyI GAGTC 2 cut(s) 14, 206
MnlI CCTC 3 cut(s) 19, 100, 478
Mox20I TGGCCA 1 cut(s) 348
Mph1103I ATGCAT 1 cut(s) 522
MroXI GAANNNNTTC 1 cut(s) 164
MscI TGGCCA 1 cut(s) 348
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 1 cut(s) 477
Msp20I TGGCCA 1 cut(s) 348
MspA1I CMGCKG 2 cut(s) 398, 530
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 451
MwoI GCNNNNNNNGC 3 cut(s) 395, 521, 530
NciI CCSGG 1 cut(s) 315
NdeII GATC 3 cut(s) 307, 372, 475
NlaIII CATG 4 cut(s) 476, 482, 491, 524
NlaIV GGNNCC 3 cut(s) 111, 571, 572
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 3 cut(s) 86, 125, 226
NsiI ATGCAT 1 cut(s) 522
NspI RCATGY 1 cut(s) 524
PaeI GCATGC 1 cut(s) 524
PagI TCATGA 1 cut(s) 472
PcsI WCGNNNNNNNCGW 1 cut(s) 263
PctI GAATGC 1 cut(s) 451
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 1 cut(s) 484
PkrI GCNGC 5 cut(s) 400, 403, 526, 529, 532
PleI GAGTC 2 cut(s) 13, 206
PpsI GAGTC 2 cut(s) 13, 206
PpuMI RGGWCCY 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 109
PspN4I GGNNCC 3 cut(s) 111, 571, 572
PspOMI GGGCCC 1 cut(s) 570
PspPI GGNCC 3 cut(s) 109, 570, 571
PspPPI RGGWCCY 1 cut(s) 109
PstI CTGCAG 1 cut(s) 529
PstNI CAGNNNCTG 1 cut(s) 401
PvuII CAGCTG 2 cut(s) 398, 530
RsaI GTAC 3 cut(s) 70, 140, 242
RsaNI GTAC 3 cut(s) 69, 139, 241
RseI CAYNNNNRTG 1 cut(s) 477
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 5 cut(s) 399, 402, 525, 528, 531
Sau3AI GATC 3 cut(s) 307, 372, 475
Sau96I GGNCC 3 cut(s) 109, 570, 571
ScaI AGTACT 1 cut(s) 70
SchI GAGTC 2 cut(s) 14, 206
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 1 cut(s) 574
SetI ASST 6 cut(s) 54, 111, 167, 400, 427, 532
SfaNI GCATC 1 cut(s) 398
SfcI CTRYAG 1 cut(s) 525
SinI GGWCC 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 477
SphI GCATGC 1 cut(s) 524
Sse9I AATT 3 cut(s) 187, 193, 302
SsiI CCGC 1 cut(s) 276
SspMI CTAG 1 cut(s) 583
StyD4I CCNGG 1 cut(s) 313
TaqI TCGA 1 cut(s) 310
TasI AATT 3 cut(s) 187, 193, 302
TatI WGTACW 2 cut(s) 68, 138
TfiI GAWTC 1 cut(s) 484
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 2 cut(s) 300, 356
TseFI GTSAC 3 cut(s) 86, 125, 226
TseI GCWGC 5 cut(s) 398, 401, 524, 527, 530
Tsp45I GTSAC 3 cut(s) 86, 125, 226
TspDTI ATGAA 1 cut(s) 319
TspRI CASTG 2 cut(s) 300, 356
VpaK11BI GGWCC 1 cut(s) 109
XceI RCATGY 1 cut(s) 524
XmnI GAANNNNTTC 1 cut(s) 164
XspI CTAG 1 cut(s) 583
ZrmI AGTACT 1 cut(s) 70
Zsp2I ATGCAT 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.