FvH4_5g12860

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001969
Physical Location & Seq
Forward (+)
7266096 .. 7267037
942 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12860.t1

Sequence Viewer

Length: 507 bp
ATGGAACCCTCCAAAGTGTTCGTCTTGCTTTGTCTTCTTTCCACCCTCCATTTCCTTGTTGTGAGTTGCGTCCAGTTTGAAGTTGGAGGCACGAATGGATGGAAGGTCCCCAAATCCAAGGAGGAATACAACCAATGGGCGTCGAAAAACCGGTTCAAAGTCGACGACACTGTTCACTTCAACTACAGTAAAGACTCGGTGATGGTGGTGACGGAGGAGGAGTACGACAAGTGCAAATCGGAACGTCCGATATTCTACTCCAAGGATGGAAACACCGAGTACAAGTTGAACCGGCCGGGGTTGTTCTACTTCATGAGTGGGGTGAAAGGGCACTGTGAGAAAGGCCAAAGGATGATCATCAAGGTCTTGGAAGTGGAACCGGCGAGCCCTCCTCCTCAGGCTGCAACAGAGTTGCAGAAGAAGAAGAATAGTGCTGCTGGAATAGCTGTTTCTTCAGCAACTGTTGTGCTATGCTTTGTGTCATTTGTTGCGGTTTTCATGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

169

Amino Acids

18.94

Weight (kDa)

8.53

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 34 - 116 5.3e-22 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 162
AciI CCGC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 293
AcuI CTGAAG 1 cut(s) 438
AcyI GRCGYC 1 cut(s) 140
AfaI GTAC 2 cut(s) 224, 281
AgeI ACCGGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 80, 157, 181, 289
AluBI AGCT 1 cut(s) 446
AluI AGCT 1 cut(s) 446
AlwNI CAGNNNCTG 1 cut(s) 461
AoxI GGCC 2 cut(s) 293, 343
ApeKI GCWGC 2 cut(s) 401, 434
ArsI GACNNNNNNTTYG 1 cut(s) 38
AsiGI ACCGGT 1 cut(s) 150
AspS9I GGNCC 1 cut(s) 106
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 3 cut(s) 211, 220, 334
AvaII GGWCC 1 cut(s) 106
AxyI CCTNAGG 1 cut(s) 396
BaeGI GKGCMC 1 cut(s) 333
BanII GRGCYC 1 cut(s) 389
BbsI GAAGAC 1 cut(s) 26
BbvI GCAGC 2 cut(s) 388, 421
BccI CCATC 3 cut(s) 93, 196, 260
BclI TGATCA 1 cut(s) 354
BcnI CCSGG 1 cut(s) 297
BfmI CTRYAG 1 cut(s) 184
BisI GCNGC 2 cut(s) 402, 435
BlsI GCNGC 2 cut(s) 403, 436
Bme1390I CCNGG 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 3 cut(s) 6, 108, 378
BmrFI CCNGG 1 cut(s) 297
BpiI GAAGAC 1 cut(s) 26
BplI GAGNNNNNCTC 2 cut(s) 376, 408
BpuMI CCSGG 1 cut(s) 297
BsaBI GATNNNNATC 1 cut(s) 356
BsaHI GRCGYC 1 cut(s) 140
BsaJI CCNNGG 3 cut(s) 117, 261, 296
BsaWI WCCGGW 1 cut(s) 150
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bse118I RCCGGY 3 cut(s) 150, 291, 379
Bse1I ACTGG 1 cut(s) 73
Bse21I CCTNAGG 1 cut(s) 396
Bse8I GATNNNNATC 1 cut(s) 356
BseDI CCNNGG 3 cut(s) 117, 261, 296
BseGI GGATG 3 cut(s) 104, 271, 357
BseJI GATNNNNATC 1 cut(s) 356
BseMII CTCAG 1 cut(s) 410
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 4 cut(s) 230, 233, 381, 384
BseSI GKGCMC 1 cut(s) 333
BseX3I CGGCCG 1 cut(s) 293
BseXI GCAGC 2 cut(s) 388, 421
Bsh1285I CGRYCG 1 cut(s) 296
BshFI GGCC 2 cut(s) 295, 345
BshTI ACCGGT 1 cut(s) 150
BsiEI CGRYCG 1 cut(s) 296
BsiSI CCGG 4 cut(s) 151, 292, 296, 380
BslFI GGGAC 1 cut(s) 92
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 2 cut(s) 295, 345
Bsp1286I GDGCHC 2 cut(s) 333, 389
Bsp143I GATC 1 cut(s) 354
BspACI CCGC 1 cut(s) 491
BspANI GGCC 2 cut(s) 295, 345
BspCNI CTCAG 1 cut(s) 409
BspHI TCATGA 1 cut(s) 312
BspLI GGNNCC 3 cut(s) 6, 108, 378
BsrFI RCCGGY 3 cut(s) 150, 291, 379
BsrI ACTGG 1 cut(s) 73
BssAI RCCGGY 3 cut(s) 150, 291, 379
BssECI CCNNGG 3 cut(s) 117, 261, 296
BssMI GATC 1 cut(s) 354
BssNI GRCGYC 1 cut(s) 140
BssT1I CCWWGG 2 cut(s) 117, 261
Bst4CI ACNGT 4 cut(s) 172, 188, 335, 463
BstACI GRCGYC 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 385
BstDEI CTNAG 1 cut(s) 396
BstF5I GGATG 3 cut(s) 104, 271, 357
BstKTI GATC 1 cut(s) 357
BstMBI GATC 1 cut(s) 354
BstMCI CGRYCG 1 cut(s) 296
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstSCI CCNGG 1 cut(s) 295
BstSFI CTRYAG 1 cut(s) 184
BstSLI GKGCMC 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 388, 421
BstV2I GAAGAC 1 cut(s) 26
BstZI CGGCCG 1 cut(s) 293
Bsu36I CCTNAGG 1 cut(s) 396
BsuRI GGCC 2 cut(s) 295, 345
BtsCI GGATG 3 cut(s) 104, 271, 357
BtsIMutI CAGTG 2 cut(s) 168, 331
Cac8I GCNNGC 1 cut(s) 385
CaiI CAGNNNCTG 1 cut(s) 461
CciI TCATGA 1 cut(s) 312
Cfr10I RCCGGY 3 cut(s) 150, 291, 379
Cfr13I GGNCC 1 cut(s) 106
CseI GACGC 2 cut(s) 58, 129
Csp6I GTAC 2 cut(s) 223, 280
CspAI ACCGGT 1 cut(s) 150
CviAII CATG 2 cut(s) 313, 499
CviJI RGCY 5 cut(s) 295, 345, 387, 401, 446
CviKI_1 RGCY 5 cut(s) 295, 345, 387, 401, 446
CviQI GTAC 2 cut(s) 223, 280
DdeI CTNAG 1 cut(s) 396
DpnI GATC 1 cut(s) 356
DpnII GATC 1 cut(s) 354
EaeI YGGCCR 1 cut(s) 293
EagI CGGCCG 1 cut(s) 293
EclXI CGGCCG 1 cut(s) 293
Eco130I CCWWGG 2 cut(s) 117, 261
Eco24I GRGCYC 1 cut(s) 389
Eco47I GGWCC 1 cut(s) 106
Eco52I CGGCCG 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 438
Eco81I CCTNAGG 1 cut(s) 396
EcoO109I RGGNCCY 1 cut(s) 106
EcoT14I CCWWGG 2 cut(s) 117, 261
EcoT38I GRGCYC 1 cut(s) 389
ErhI CCWWGG 2 cut(s) 117, 261
FaeI CATG 2 cut(s) 316, 502
FaiI YATR 3 cut(s) 314, 472, 500
FaqI GGGAC 1 cut(s) 92
FatI CATG 2 cut(s) 312, 498
FbaI TGATCA 1 cut(s) 354
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 2 cut(s) 402, 435
FokI GGATG 3 cut(s) 111, 278, 364
FriOI GRGCYC 1 cut(s) 389
Fsp4HI GCNGC 2 cut(s) 402, 435
GluI GCNGC 2 cut(s) 402, 435
HaeIII GGCC 2 cut(s) 295, 345
HapII CCGG 4 cut(s) 151, 292, 296, 380
HgaI GACGC 2 cut(s) 58, 129
Hin1I GRCGYC 1 cut(s) 140
Hin1II CATG 2 cut(s) 316, 502
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 1 cut(s) 194
HpaII CCGG 4 cut(s) 151, 292, 296, 380
HphI GGTGA 3 cut(s) 211, 220, 334
Hpy166II GTNNAC 2 cut(s) 163, 175
Hpy188I TCNGA 2 cut(s) 241, 249
Hpy188III TCNNGA 1 cut(s) 313
Hpy8I GTNNAC 2 cut(s) 163, 175
Hpy99I CGWCG 2 cut(s) 145, 167
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 4 cut(s) 172, 188, 335, 463
HpyCH4IV ACGT 1 cut(s) 244
HpyCH4V TGCA 3 cut(s) 234, 404, 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 1 cut(s) 244
Hsp92I GRCGYC 1 cut(s) 140
Hsp92II CATG 2 cut(s) 316, 502
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 1 cut(s) 354
LpnPI CCDG 7 cut(s) 86, 164, 305, 309, 383, 393, 423
Lsp1109I GCAGC 2 cut(s) 388, 421
MaeII ACGT 1 cut(s) 244
MaeIII GTNAC 1 cut(s) 208
MalI GATC 1 cut(s) 356
MboI GATC 1 cut(s) 354
MboII GAAGA 5 cut(s) 26, 430, 433, 436, 444
MhlI GDGCHC 2 cut(s) 333, 389
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 9 cut(s) 19, 56, 80, 115, 208, 211, 399, 402, 405
MspI CCGG 4 cut(s) 151, 292, 296, 380
MspR9I CCNGG 1 cut(s) 297
MwoI GCNNNNNNNGC 1 cut(s) 443
NciI CCSGG 1 cut(s) 297
NdeII GATC 1 cut(s) 354
NlaIII CATG 2 cut(s) 316, 502
NlaIV GGNNCC 3 cut(s) 6, 108, 378
NmuCI GTSAC 1 cut(s) 208
PagI TCATGA 1 cut(s) 312
PcsI WCGNNNNNNNCGW 1 cut(s) 245
PinAI ACCGGT 1 cut(s) 150
PkrI GCNGC 2 cut(s) 403, 436
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
PpuMI RGGWCCY 1 cut(s) 106
Psp5II RGGWCCY 1 cut(s) 106
PspN4I GGNNCC 3 cut(s) 6, 108, 378
PspPI GGNCC 1 cut(s) 106
PspPPI RGGWCCY 1 cut(s) 106
PstNI CAGNNNCTG 1 cut(s) 461
RsaI GTAC 2 cut(s) 224, 281
RsaNI GTAC 2 cut(s) 223, 280
SalI GTCGAC 1 cut(s) 161
SatI GCNGC 2 cut(s) 402, 435
Sau3AI GATC 1 cut(s) 354
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 1 cut(s) 297
SduI GDGCHC 2 cut(s) 333, 389
SetI ASST 4 cut(s) 108, 247, 366, 448
SfcI CTRYAG 1 cut(s) 184
SinI GGWCC 1 cut(s) 106
SsiI CCGC 1 cut(s) 491
StyD4I CCNGG 1 cut(s) 295
StyI CCWWGG 2 cut(s) 117, 261
TaaI ACNGT 4 cut(s) 172, 188, 335, 463
TaiI ACGT 1 cut(s) 247
TaqI TCGA 2 cut(s) 143, 162
TatI WGTACW 1 cut(s) 279
TscAI CASTG 2 cut(s) 175, 338
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 2 cut(s) 401, 434
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 301, 487
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 2 cut(s) 175, 338
VpaK11BI GGWCC 1 cut(s) 106
XcmI CCANNNNNNNNNTGG 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.