Rw7G001840

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001969
Physical Location & Seq
Reverse (-)
1494619 .. 1495293
675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001840.1

Sequence Viewer

Length: 576 bp
ATGGAATCCTCCAAAGTATTTCTCTTGTTTTTTCTTCTCTTTGCTATTCAGGTCGTCTTTGTAACTTGTACTCAATTTCAAGTTGGTGAGAAGAGCAATGGATGGGAGGTCCCCAAATCAAAGAGTGACCAAGATATGTACAACCAATGGGCCTCTAAGAATAGGTTCAAAGTTGACGACACTCTCAATTTCAGTTACAAGAAAAACTCAGACTCAGTCATGGTCGTGACGGAAGCTGAGTACGAAAAATGCCATTCCGATAATCCCATTTTCAGCTCTAACGATGGTGACTCGGTCTTCAAATTGGATCGACCCGGTTCGTTCTACTTCATTAGTGGGACTGCCGGACACTGCGAGAAAGGGCAGAAAATGATTGTCAAAGTCTTGGGATCACCGGCTGCTGGAACAGAAAGCCCACCACCTGCTCAATCAGCAAACCAGAATGCAACTGAATCACCAGATCATCATGATCATGATCATCATGACATGAAGAACAATGCAGTTGCAATGCATGCTGTAGCTGCCATCTCTTTTACAACTTCTGTAATGTCATTTCTTGGGGTCTTTTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

191

Amino Acids

21.24

Weight (kDa)

5.69

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 40 - 122 2.5e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 430
Acc36I ACCTGC 1 cut(s) 430
AclWI GGATC 2 cut(s) 315, 397
AfaI GTAC 3 cut(s) 70, 140, 242
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 3 cut(s) 80, 169, 301
AluBI AGCT 3 cut(s) 236, 276, 521
AluI AGCT 3 cut(s) 236, 276, 521
AlwI GGATC 2 cut(s) 315, 397
AoxI GGCC 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 398, 521
Asp700I GAANNNNTTC 1 cut(s) 164
AspS9I GGNCC 2 cut(s) 109, 150
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 4 cut(s) 98, 299, 384, 447
AvaII GGWCC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 289
BbvI GCAGC 2 cut(s) 385, 508
BccI CCATC 3 cut(s) 96, 278, 533
BclI TGATCA 2 cut(s) 469, 475
BcnI CCSGG 1 cut(s) 315
BfaI CTAG 1 cut(s) 574
BfmI CTRYAG 1 cut(s) 516
BfuAI ACCTGC 1 cut(s) 430
BisI GCNGC 2 cut(s) 399, 522
BlsI GCNGC 2 cut(s) 400, 523
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 2 cut(s) 109, 150
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 1 cut(s) 315
BpiI GAAGAC 1 cut(s) 289
BpuMI CCSGG 1 cut(s) 315
BsaBI GATNNNNATC 1 cut(s) 474
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse118I RCCGGY 1 cut(s) 394
Bse3DI GCAATG 2 cut(s) 103, 513
Bse8I GATNNNNATC 1 cut(s) 474
BseGI GGATG 1 cut(s) 107
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 2 cut(s) 103, 513
BseMII CTCAG 3 cut(s) 222, 228, 228
BseXI GCAGC 2 cut(s) 385, 508
BshFI GGCC 1 cut(s) 152
BsiSI CCGG 3 cut(s) 315, 345, 395
BslFI GGGAC 2 cut(s) 95, 352
BslI CCNNNNNNNGG 1 cut(s) 401
BsmFI GGGAC 2 cut(s) 95, 352
BsmI GAATGC 1 cut(s) 448
BsnI GGCC 1 cut(s) 152
Bsp1407I TGTACA 1 cut(s) 138
Bsp143I GATC 5 cut(s) 307, 389, 460, 469, 475
BspANI GGCC 1 cut(s) 152
BspCNI CTCAG 3 cut(s) 221, 227, 229
BspHI TCATGA 3 cut(s) 466, 472, 481
BspLI GGNNCC 1 cut(s) 111
BspMI ACCTGC 1 cut(s) 430
BspPI GGATC 2 cut(s) 315, 397
BspQI GCTCTTC 1 cut(s) 86
BsrDI GCAATG 2 cut(s) 103, 513
BsrFI RCCGGY 1 cut(s) 394
BsrGI TGTACA 1 cut(s) 138
BssAI RCCGGY 1 cut(s) 394
BssMI GATC 5 cut(s) 307, 389, 460, 469, 475
Bst6I CTCTTC 1 cut(s) 86
BstAPI GCANNNNNTGC 1 cut(s) 512
BstAUI TGTACA 1 cut(s) 138
BstC8I GCNNGC 1 cut(s) 513
BstDEI CTNAG 4 cut(s) 156, 208, 214, 237
BstF5I GGATG 1 cut(s) 107
BstKTI GATC 5 cut(s) 310, 392, 463, 472, 478
BstMBI GATC 5 cut(s) 307, 389, 460, 469, 475
BstMWI GCNNNNNNNGC 3 cut(s) 431, 512, 521
BstNSI RCATGY 1 cut(s) 515
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 516
BstV1I GCAGC 2 cut(s) 385, 508
BstV2I GAAGAC 1 cut(s) 289
BsuRI GGCC 1 cut(s) 152
BtsCI GGATG 1 cut(s) 107
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 1 cut(s) 349
BveI ACCTGC 1 cut(s) 430
Cac8I GCNNGC 1 cut(s) 513
CciI TCATGA 3 cut(s) 466, 472, 481
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 2 cut(s) 109, 150
Csp6I GTAC 3 cut(s) 69, 139, 241
CviAII CATG 6 cut(s) 220, 467, 473, 482, 487, 512
CviJI RGCY 6 cut(s) 152, 236, 276, 398, 414, 521
CviKI_1 RGCY 6 cut(s) 152, 236, 276, 398, 414, 521
CviQI GTAC 3 cut(s) 69, 139, 241
DdeI CTNAG 4 cut(s) 156, 208, 214, 237
DpnI GATC 5 cut(s) 309, 391, 462, 471, 477
DpnII GATC 5 cut(s) 307, 389, 460, 469, 475
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
Eco47I GGWCC 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 109
EcoT22I ATGCAT 1 cut(s) 513
FaeI CATG 6 cut(s) 223, 470, 476, 485, 490, 515
FaiI YATR 7 cut(s) 137, 221, 468, 474, 483, 488, 513
FaqI GGGAC 2 cut(s) 95, 352
FatI CATG 6 cut(s) 219, 466, 472, 481, 486, 511
FbaI TGATCA 2 cut(s) 469, 475
Fnu4HI GCNGC 2 cut(s) 399, 522
FokI GGATG 1 cut(s) 114
Fsp4HI GCNGC 2 cut(s) 399, 522
FspBI CTAG 1 cut(s) 574
GluI GCNGC 2 cut(s) 399, 522
HaeIII GGCC 1 cut(s) 152
HapII CCGG 3 cut(s) 315, 345, 395
Hin1II CATG 6 cut(s) 223, 470, 476, 485, 490, 515
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 4 cut(s) 5, 212, 290, 452
HpaII CCGG 3 cut(s) 315, 345, 395
HphI GGTGA 4 cut(s) 98, 299, 384, 447
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 2 cut(s) 211, 259
Hpy188III TCNNGA 4 cut(s) 226, 467, 473, 482
Hpy8I GTNNAC 1 cut(s) 175
HpyCH4V TGCA 4 cut(s) 446, 500, 506, 511
HpyF10VI GCNNNNNNNGC 3 cut(s) 431, 512, 521
HpyF3I CTNAG 4 cut(s) 156, 208, 214, 237
Hsp92II CATG 6 cut(s) 223, 470, 476, 485, 490, 515
Ksp22I TGATCA 2 cut(s) 469, 475
Kzo9I GATC 5 cut(s) 307, 389, 460, 469, 475
LguI GCTCTTC 1 cut(s) 86
LpnPI CCDG 8 cut(s) 35, 328, 358, 387, 408, 435, 452, 471
Lsp1109I GCAGC 2 cut(s) 385, 508
MaeI CTAG 1 cut(s) 574
MaeIII GTNAC 5 cut(s) 61, 125, 194, 226, 287
MalI GATC 5 cut(s) 309, 391, 462, 471, 477
MboI GATC 5 cut(s) 307, 389, 460, 469, 475
MboII GAAGA 5 cut(s) 26, 103, 289, 502, 562
MluCI AATT 3 cut(s) 74, 187, 302
MlyI GAGTC 2 cut(s) 206, 284
MnlI CCTC 3 cut(s) 19, 100, 163
Mph1103I ATGCAT 1 cut(s) 513
MroXI GAANNNNTTC 1 cut(s) 164
MslI CAYNNNNRTG 2 cut(s) 224, 471
MspI CCGG 3 cut(s) 315, 345, 395
MspR9I CCNGG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 448
MwoI GCNNNNNNNGC 3 cut(s) 431, 512, 521
NciI CCSGG 1 cut(s) 315
NdeII GATC 5 cut(s) 307, 389, 460, 469, 475
NlaIII CATG 6 cut(s) 223, 470, 476, 485, 490, 515
NlaIV GGNNCC 1 cut(s) 111
NmuCI GTSAC 3 cut(s) 125, 226, 287
NsiI ATGCAT 1 cut(s) 513
NspI RCATGY 1 cut(s) 515
PaeI GCATGC 1 cut(s) 515
PagI TCATGA 3 cut(s) 466, 472, 481
PaqCI CACCTGC 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 86
PctI GAATGC 1 cut(s) 448
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 2 cut(s) 5, 452
PflFI GACNNNGTC 2 cut(s) 215, 293
PkrI GCNGC 2 cut(s) 400, 523
PleI GAGTC 2 cut(s) 206, 284
PpsI GAGTC 2 cut(s) 206, 284
PpuMI RGGWCCY 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 2 cut(s) 109, 150
PspPPI RGGWCCY 1 cut(s) 109
PsyI GACNNNGTC 2 cut(s) 215, 293
RsaI GTAC 3 cut(s) 70, 140, 242
RsaNI GTAC 3 cut(s) 69, 139, 241
RseI CAYNNNNRTG 2 cut(s) 224, 471
SapI GCTCTTC 1 cut(s) 86
SatI GCNGC 2 cut(s) 399, 522
Sau3AI GATC 5 cut(s) 307, 389, 460, 469, 475
Sau96I GGNCC 2 cut(s) 109, 150
SchI GAGTC 2 cut(s) 206, 284
ScrFI CCNGG 1 cut(s) 315
SetI ASST 7 cut(s) 54, 111, 167, 238, 278, 424, 523
SfcI CTRYAG 1 cut(s) 516
SinI GGWCC 1 cut(s) 109
SmiMI CAYNNNNRTG 2 cut(s) 224, 471
SphI GCATGC 1 cut(s) 515
Sse9I AATT 3 cut(s) 74, 187, 302
SspMI CTAG 1 cut(s) 574
StyD4I CCNGG 1 cut(s) 313
TaqI TCGA 1 cut(s) 310
TaqII GACCGA 1 cut(s) 283
TasI AATT 3 cut(s) 74, 187, 302
TatI WGTACW 2 cut(s) 68, 138
TfiI GAWTC 2 cut(s) 5, 452
TscAI CASTG 1 cut(s) 356
TseFI GTSAC 3 cut(s) 125, 226, 287
TseI GCWGC 2 cut(s) 398, 521
Tsp45I GTSAC 3 cut(s) 125, 226, 287
TspDTI ATGAA 2 cut(s) 319, 503
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 1 cut(s) 356
Tth111I GACNNNGTC 2 cut(s) 215, 293
VpaK11BI GGWCC 1 cut(s) 109
XceI RCATGY 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 164
XspI CTAG 1 cut(s) 574
Zsp2I ATGCAT 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.