RchiOBHm_Chr7g0179281

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001969
Physical Location & Seq
Reverse (-)
1617528 .. 1618202
675 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15980

Sequence Viewer

Length: 546 bp
ATGGAATCCTCCAAAGTATTTCTCTTGTTTTTTCTTCTCTTTGCTATTCAGGTCGTTTTTGTAACTTGTACTCAATTTCAAGTTGGTGACAAGAGCAATGGATGGGAGGTCCCCAAATCAAAGAGTGACCAAGATATGTACAACCAATGGGCCTCTAAGAATAGGTTCAAAGTTGACGACACTCTCATTATGGTGGTGACGGAAGCTGAGTACGAAAAATGCCATTCCGATAATCCCATTTTCAGCTCTAACGATGGTGACTTAGTCTTCAAATTGGATCGACCCGGTTCGTTCTACTTCATTAGTGGGACTGCCGGACACTGCGAGAAAGGGCAGAAAATGATTGTCAAAGTCTTGGGATCACCGGCTGCTGGAACAGAAAGCCCACCACCTGCTCAATCAGCAAACCAGAATGCAACTGAATCACCAGATCATCATGATCATGATCATCATGACATGAAGAACAATGCAGTTGCAATGCATGCTGTAACTGCCATCTCTTTTACAACTTCTGTAATGTCATTTCTTGGGGTCTTTTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

181

Amino Acids

20.12

Weight (kDa)

5.52

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 40 - 112 3.3e-13 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 400
Acc36I ACCTGC 1 cut(s) 400
AclWI GGATC 2 cut(s) 285, 367
AfaI GTAC 3 cut(s) 70, 140, 212
AfiI CCNNNNNNNGG 1 cut(s) 371
AgsI TTSAA 3 cut(s) 80, 169, 271
AluBI AGCT 2 cut(s) 206, 246
AluI AGCT 2 cut(s) 206, 246
AlwI GGATC 2 cut(s) 285, 367
AoxI GGCC 1 cut(s) 150
ApeKI GCWGC 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 164
AspS9I GGNCC 2 cut(s) 109, 150
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 5 cut(s) 98, 208, 269, 354, 417
AvaII GGWCC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 259
BbvI GCAGC 1 cut(s) 355
BccI CCATC 3 cut(s) 96, 248, 503
BclI TGATCA 2 cut(s) 439, 445
BcnI CCSGG 1 cut(s) 285
BfaI CTAG 1 cut(s) 544
BfuAI ACCTGC 1 cut(s) 400
BisI GCNGC 1 cut(s) 369
BlsI GCNGC 1 cut(s) 370
Bme1390I CCNGG 1 cut(s) 285
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 2 cut(s) 109, 150
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 1 cut(s) 285
BpiI GAAGAC 1 cut(s) 259
BpuMI CCSGG 1 cut(s) 285
BsaBI GATNNNNATC 1 cut(s) 444
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse118I RCCGGY 1 cut(s) 364
Bse3DI GCAATG 2 cut(s) 103, 483
Bse8I GATNNNNATC 1 cut(s) 444
BseGI GGATG 1 cut(s) 107
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMI GCAATG 2 cut(s) 103, 483
BseMII CTCAG 1 cut(s) 198
BseXI GCAGC 1 cut(s) 355
BshFI GGCC 1 cut(s) 152
BsiSI CCGG 3 cut(s) 285, 315, 365
BslFI GGGAC 2 cut(s) 95, 322
BslI CCNNNNNNNGG 1 cut(s) 371
BsmFI GGGAC 2 cut(s) 95, 322
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 1 cut(s) 152
Bsp1407I TGTACA 1 cut(s) 138
Bsp143I GATC 5 cut(s) 277, 359, 430, 439, 445
BspANI GGCC 1 cut(s) 152
BspCNI CTCAG 1 cut(s) 199
BspHI TCATGA 3 cut(s) 436, 442, 451
BspLI GGNNCC 1 cut(s) 111
BspMI ACCTGC 1 cut(s) 400
BspPI GGATC 2 cut(s) 285, 367
BsrDI GCAATG 2 cut(s) 103, 483
BsrFI RCCGGY 1 cut(s) 364
BsrGI TGTACA 1 cut(s) 138
BssAI RCCGGY 1 cut(s) 364
BssMI GATC 5 cut(s) 277, 359, 430, 439, 445
BstAPI GCANNNNNTGC 1 cut(s) 482
BstAUI TGTACA 1 cut(s) 138
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 3 cut(s) 156, 207, 262
BstF5I GGATG 1 cut(s) 107
BstKTI GATC 5 cut(s) 280, 362, 433, 442, 448
BstMBI GATC 5 cut(s) 277, 359, 430, 439, 445
BstMWI GCNNNNNNNGC 3 cut(s) 401, 482, 491
BstNSI RCATGY 1 cut(s) 485
BstSCI CCNGG 1 cut(s) 283
BstV1I GCAGC 1 cut(s) 355
BstV2I GAAGAC 1 cut(s) 259
BsuRI GGCC 1 cut(s) 152
BtsCI GGATG 1 cut(s) 107
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 319
BveI ACCTGC 1 cut(s) 400
Cac8I GCNNGC 1 cut(s) 483
CciI TCATGA 3 cut(s) 436, 442, 451
Cfr10I RCCGGY 1 cut(s) 364
Cfr13I GGNCC 2 cut(s) 109, 150
Csp6I GTAC 3 cut(s) 69, 139, 211
CviAII CATG 5 cut(s) 437, 443, 452, 457, 482
CviJI RGCY 5 cut(s) 152, 206, 246, 368, 384
CviKI_1 RGCY 5 cut(s) 152, 206, 246, 368, 384
CviQI GTAC 3 cut(s) 69, 139, 211
DdeI CTNAG 3 cut(s) 156, 207, 262
DpnI GATC 5 cut(s) 279, 361, 432, 441, 447
DpnII GATC 5 cut(s) 277, 359, 430, 439, 445
Eco47I GGWCC 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 109
EcoT22I ATGCAT 1 cut(s) 483
FaeI CATG 5 cut(s) 440, 446, 455, 460, 485
FaiI YATR 7 cut(s) 137, 191, 438, 444, 453, 458, 483
FaqI GGGAC 2 cut(s) 95, 322
FatI CATG 5 cut(s) 436, 442, 451, 456, 481
FbaI TGATCA 2 cut(s) 439, 445
Fnu4HI GCNGC 1 cut(s) 369
FokI GGATG 1 cut(s) 114
Fsp4HI GCNGC 1 cut(s) 369
FspBI CTAG 1 cut(s) 544
GluI GCNGC 1 cut(s) 369
HaeIII GGCC 1 cut(s) 152
HapII CCGG 3 cut(s) 285, 315, 365
Hin1II CATG 5 cut(s) 440, 446, 455, 460, 485
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 2 cut(s) 5, 422
HpaII CCGG 3 cut(s) 285, 315, 365
HphI GGTGA 5 cut(s) 98, 208, 269, 354, 417
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 1 cut(s) 229
Hpy188III TCNNGA 3 cut(s) 437, 443, 452
Hpy8I GTNNAC 1 cut(s) 175
HpyCH4V TGCA 4 cut(s) 416, 470, 476, 481
HpyF10VI GCNNNNNNNGC 3 cut(s) 401, 482, 491
HpyF3I CTNAG 3 cut(s) 156, 207, 262
Hsp92II CATG 5 cut(s) 440, 446, 455, 460, 485
Ksp22I TGATCA 2 cut(s) 439, 445
Kzo9I GATC 5 cut(s) 277, 359, 430, 439, 445
LpnPI CCDG 8 cut(s) 35, 298, 328, 357, 378, 405, 422, 441
Lsp1109I GCAGC 1 cut(s) 355
MaeI CTAG 1 cut(s) 544
MaeIII GTNAC 6 cut(s) 61, 86, 125, 196, 257, 487
MalI GATC 5 cut(s) 279, 361, 432, 441, 447
MboI GATC 5 cut(s) 277, 359, 430, 439, 445
MboII GAAGA 4 cut(s) 26, 259, 472, 532
MluCI AATT 2 cut(s) 74, 272
MnlI CCTC 3 cut(s) 19, 100, 163
Mph1103I ATGCAT 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 164
MslI CAYNNNNRTG 2 cut(s) 191, 441
MspI CCGG 3 cut(s) 285, 315, 365
MspR9I CCNGG 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 418
MwoI GCNNNNNNNGC 3 cut(s) 401, 482, 491
NciI CCSGG 1 cut(s) 285
NdeII GATC 5 cut(s) 277, 359, 430, 439, 445
NlaIII CATG 5 cut(s) 440, 446, 455, 460, 485
NlaIV GGNNCC 1 cut(s) 111
NmuCI GTSAC 4 cut(s) 86, 125, 196, 257
NsiI ATGCAT 1 cut(s) 483
NspI RCATGY 1 cut(s) 485
PaeI GCATGC 1 cut(s) 485
PagI TCATGA 3 cut(s) 436, 442, 451
PaqCI CACCTGC 1 cut(s) 400
PctI GAATGC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 2 cut(s) 5, 422
PflFI GACNNNGTC 1 cut(s) 263
PkrI GCNGC 1 cut(s) 370
PpuMI RGGWCCY 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 2 cut(s) 109, 150
PspPPI RGGWCCY 1 cut(s) 109
PsyI GACNNNGTC 1 cut(s) 263
RsaI GTAC 3 cut(s) 70, 140, 212
RsaNI GTAC 3 cut(s) 69, 139, 211
RseI CAYNNNNRTG 2 cut(s) 191, 441
SatI GCNGC 1 cut(s) 369
Sau3AI GATC 5 cut(s) 277, 359, 430, 439, 445
Sau96I GGNCC 2 cut(s) 109, 150
ScrFI CCNGG 1 cut(s) 285
SetI ASST 6 cut(s) 54, 111, 167, 208, 248, 394
SinI GGWCC 1 cut(s) 109
SmiMI CAYNNNNRTG 2 cut(s) 191, 441
SphI GCATGC 1 cut(s) 485
Sse9I AATT 2 cut(s) 74, 272
SspMI CTAG 1 cut(s) 544
StyD4I CCNGG 1 cut(s) 283
TaqI TCGA 1 cut(s) 280
TasI AATT 2 cut(s) 74, 272
TatI WGTACW 2 cut(s) 68, 138
TfiI GAWTC 2 cut(s) 5, 422
TscAI CASTG 1 cut(s) 326
TseFI GTSAC 4 cut(s) 86, 125, 196, 257
TseI GCWGC 1 cut(s) 368
Tsp45I GTSAC 4 cut(s) 86, 125, 196, 257
TspDTI ATGAA 2 cut(s) 289, 473
TspGWI ACGGA 1 cut(s) 215
TspRI CASTG 1 cut(s) 326
Tth111I GACNNNGTC 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 109
XceI RCATGY 1 cut(s) 485
XmnI GAANNNNTTC 1 cut(s) 164
XspI CTAG 1 cut(s) 544
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.