FvH4_5g12850

Piriformospora indica-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001969
Physical Location & Seq
Forward (+)
7264094 .. 7265685
1592 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12850.t1

Sequence Viewer

Length: 1287 bp
ATGAAGCTTTTTGTTCTGCTCTTGTTCTTCTGGTGCAGCGCCTTTTTTGTATTGCTTGTAGTGCAAGCAGACCCTTCAGTTTCACTTTCTCCTATGCTGAAAAAAGAGCAACAAGCCCTTTACTCTACCATCCAAGGCTTTGTGGGGAACTGGTGGAATGGTTCTGATCTCTATCCAGATCCTTGTGGCTCGACTCCGATACAGGGAGTTTATTGTGATCTGTACAATGGTTTCTGGTATGTTGCTGTTATAGACATTGGACCGCTTTACGACAACTATCTTCACTGCACCCCAAATGTAGAGTTTTCACATCATCTGTTCAAGCTCAAGCACTTAAAGGTTCTCTCATTCTTCAATTGCTTCCTCTCACCAATTACAATTTCCCATTTAAACTGGGAAAAGCTTTCAAACAGTATAGAATCCTTGGAATTTCGATCAAACCCTGGTCTCATTGGGAGAATTCCAAACACTTTTGGTTACCTTAAAAAGCTACAATCTCTAGTGCTGCTAGAAAATGGGTTACAAGGCAAGTTGCCTAATCAAATAGGCAATTTAGTCCACTTGAGGCGGCTTGTCCTAGCCGGAAACAATCTTTCTGGTAGGATTCCAGCAAGTCTTGGAAGACTGACTAAGCTTTTGATACTTGATGCAAGCAGAAACCAGCTATCTGGTTCATTACCTGTGACTTTAGGATCTTTGACTTCACTTCTAAAGCTTGACTTAAGCAACAATATGATAGAGGGAAAGTTTCCTGTAGAGCTTGGAAGGCTAAAGAATCTCACACTACTAGATCTTGGCAGCAACAAAATTTCAGGTTGGTTGGTTCAGTCACTTGAAGAAATGGTTTCTATAAAACAATTGGTGATGTCCAACAATCCAATTGGGGGAAGTCTCATCAGAATTCAGTGGCAAAATCTACACAAGCTGGAAATTTTGGACCTTTCATGCACATATTTAAGAGGGCACATTCCTAAGTCCATGGCAGAAATGAAAAGGCTAAGGTTTTTGGGGCTTAACAATAACAAACTATCAGGTACAATATCTCCAAGGCTGGAATCTCTGCCTTGCATTGGTGCTCTTTACCTTTATGGAAACAATTTCACAGGAGAAGTTAAATTCTCAGAAGGGTTCTATCGGAAAATGGGGAGGCGATTTGGAGCTTGGAACAACCCGAATCTTTGTTACCAGGCTGAATCAAGTTCACCAAAGTATGTTCCTCATGGTGTAAAACCATGCCAGCAAGAAACAACTATTTCCTCTGATAAGTTTCCAAATTCCAAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8

Protein Analysis

429

Amino Acids

48.05

Weight (kDa)

9.32

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 160 - 375 1e-16 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 263, 568
AclWI GGATC 2 cut(s) 173, 700
AcsI RAATTY 7 cut(s) 428, 459, 807, 900, 930, 1115, 1273
AcuI CTGAAG 1 cut(s) 60
AfaI GTAC 2 cut(s) 224, 1036
AfiI CCNNNNNNNGG 3 cut(s) 203, 884, 1070
AflII CTTAAG 1 cut(s) 721
AgsI TTSAA 4 cut(s) 322, 355, 408, 836
AjnI CCWGG 2 cut(s) 442, 1185
AjuI GAANNNNNNNTTGG 2 cut(s) 364, 396
Alw21I GWGCWC 1 cut(s) 1078
Alw26I GTCTC 2 cut(s) 452, 896
AlwI GGATC 2 cut(s) 173, 700
ApeKI GCWGC 3 cut(s) 36, 505, 798
ApoI RAATTY 7 cut(s) 428, 459, 807, 900, 930, 1115, 1273
ArsI GACNNNNNNTTYG 2 cut(s) 619, 651
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 2 cut(s) 260, 937
AsuHPI GGTGA 3 cut(s) 360, 874, 1194
AvaII GGWCC 2 cut(s) 260, 937
BaeGI GKGCMC 1 cut(s) 966
BbsI GAAGAC 1 cut(s) 628
Bbv12I GWGCWC 1 cut(s) 1078
BbvI GCAGC 3 cut(s) 48, 492, 810
BccI CCATC 1 cut(s) 137
BciT130I CCWGG 2 cut(s) 444, 1187
BcoDI GTCTC 2 cut(s) 452, 896
BfaI CTAG 4 cut(s) 500, 509, 578, 788
BfmI CTRYAG 1 cut(s) 753
BfoI RGCGCY 1 cut(s) 42
BfrI CTTAAG 1 cut(s) 721
BglII AGATCT 1 cut(s) 790
BisI GCNGC 4 cut(s) 37, 506, 569, 799
BlsI GCNGC 4 cut(s) 38, 507, 570, 800
Bme1390I CCNGG 2 cut(s) 444, 1187
Bme18I GGWCC 2 cut(s) 260, 937
BmgT120I GGNCC 2 cut(s) 260, 937
BmrFI CCNGG 2 cut(s) 444, 1187
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 1 cut(s) 637
BmuI ACTGGG 1 cut(s) 403
BpiI GAAGAC 1 cut(s) 628
Bpu10I CCTNAGC 1 cut(s) 998
BpuEI CTTGAG 2 cut(s) 311, 583
BsaBI GATNNNNATC 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 5 cut(s) 133, 423, 442, 978, 1046
BsaXI ACNNNNNCTCC 2 cut(s) 1027, 1057
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 884, 1070
Bse1I ACTGG 2 cut(s) 155, 398
Bse8I GATNNNNATC 1 cut(s) 171
BseBI CCWGG 2 cut(s) 444, 1187
BseDI CCNNGG 5 cut(s) 133, 423, 442, 978, 1046
BseGI GGATG 1 cut(s) 129
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 3 cut(s) 203, 884, 1070
BseMII CTCAG 1 cut(s) 1134
BseNI ACTGG 2 cut(s) 155, 398
BseSI GKGCMC 1 cut(s) 966
BseXI GCAGC 3 cut(s) 48, 492, 810
BsgI GTGCAG 2 cut(s) 55, 271
BsiHKAI GWGCWC 1 cut(s) 1078
BsiSI CCGG 1 cut(s) 582
BslI CCNNNNNNNGG 3 cut(s) 203, 884, 1070
BsmAI GTCTC 2 cut(s) 452, 896
Bso31I GGTCTC 1 cut(s) 452
Bsp1286I GDGCHC 2 cut(s) 966, 1078
Bsp1407I TGTACA 1 cut(s) 222
Bsp143I GATC 6 cut(s) 166, 178, 217, 434, 692, 790
Bsp19I CCATGG 1 cut(s) 978
BspACI CCGC 2 cut(s) 263, 568
BspCNI CTCAG 1 cut(s) 1133
BspPI GGATC 2 cut(s) 173, 700
BspTI CTTAAG 1 cut(s) 721
BspTNI GGTCTC 1 cut(s) 452
BsrGI TGTACA 1 cut(s) 222
BsrI ACTGG 2 cut(s) 155, 398
BssECI CCNNGG 5 cut(s) 133, 423, 442, 978, 1046
BssMI GATC 6 cut(s) 166, 178, 217, 434, 692, 790
BssT1I CCWWGG 4 cut(s) 133, 423, 978, 1046
Bst2UI CCWGG 2 cut(s) 444, 1187
Bst4CI ACNGT 1 cut(s) 413
BstAFI CTTAAG 1 cut(s) 721
BstAUI TGTACA 1 cut(s) 222
BstC8I GCNNGC 3 cut(s) 66, 652, 1238
BstDEI CTNAG 4 cut(s) 630, 972, 998, 1120
BstDSI CCRYGG 1 cut(s) 978
BstEII GGTNACC 1 cut(s) 476
BstF5I GGATG 1 cut(s) 129
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 6 cut(s) 169, 181, 220, 437, 695, 793
BstMAI GTCTC 2 cut(s) 452, 896
BstMBI GATC 6 cut(s) 166, 178, 217, 434, 692, 790
BstMWI GCNNNNNNNGC 2 cut(s) 61, 766
BstNI CCWGG 2 cut(s) 444, 1187
BstPI GGTNACC 1 cut(s) 476
BstSCI CCNGG 2 cut(s) 442, 1185
BstSFI CTRYAG 1 cut(s) 753
BstSLI GKGCMC 1 cut(s) 966
BstV1I GCAGC 3 cut(s) 48, 492, 810
BstV2I GAAGAC 1 cut(s) 628
BstX2I RGATCY 3 cut(s) 178, 692, 790
BstXI CCANNNNNNTGG 1 cut(s) 668
BstYI RGATCY 3 cut(s) 178, 692, 790
BtgI CCRYGG 1 cut(s) 978
BtsCI GGATG 1 cut(s) 129
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 2 cut(s) 283, 911
Cac8I GCNNGC 3 cut(s) 66, 652, 1238
CfoI GCGC 1 cut(s) 41
Cfr13I GGNCC 2 cut(s) 260, 937
Csp6I GTAC 2 cut(s) 223, 1035
CviAII CATG 4 cut(s) 945, 979, 1220, 1233
CviQI GTAC 2 cut(s) 223, 1035
DdeI CTNAG 4 cut(s) 630, 972, 998, 1120
DpnI GATC 6 cut(s) 168, 180, 219, 436, 694, 792
DpnII GATC 6 cut(s) 166, 178, 217, 434, 692, 790
DraI TTTAAA 1 cut(s) 390
Eco130I CCWWGG 4 cut(s) 133, 423, 978, 1046
Eco31I GGTCTC 1 cut(s) 452
Eco47I GGWCC 2 cut(s) 260, 937
Eco57I CTGAAG 1 cut(s) 60
Eco91I GGTNACC 1 cut(s) 476
EcoO65I GGTNACC 1 cut(s) 476
EcoRI GAATTC 2 cut(s) 459, 900
EcoRII CCWGG 2 cut(s) 442, 1185
EcoT14I CCWWGG 4 cut(s) 133, 423, 978, 1046
ErhI CCWWGG 4 cut(s) 133, 423, 978, 1046
FaeI CATG 4 cut(s) 948, 982, 1223, 1236
FalI AAGNNNNNCTT 2 cut(s) 704, 736
FatI CATG 4 cut(s) 944, 978, 1219, 1232
Fnu4HI GCNGC 4 cut(s) 37, 506, 569, 799
FokI GGATG 1 cut(s) 116
Fsp4HI GCNGC 4 cut(s) 37, 506, 569, 799
FspBI CTAG 4 cut(s) 500, 509, 578, 788
GlaI GCGC 1 cut(s) 40
GluI GCNGC 4 cut(s) 37, 506, 569, 799
HaeII RGCGCY 1 cut(s) 42
HapII CCGG 1 cut(s) 582
HhaI GCGC 1 cut(s) 41
Hin1II CATG 4 cut(s) 948, 982, 1223, 1236
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HindIII AAGCTT 4 cut(s) 5, 401, 632, 713
HinfI GANTC 7 cut(s) 193, 419, 604, 775, 1055, 1174, 1193
HpaII CCGG 1 cut(s) 582
HphI GGTGA 3 cut(s) 360, 874, 1194
Hpy166II GTNNAC 2 cut(s) 559, 1202
Hpy188I TCNGA 6 cut(s) 166, 198, 899, 1123, 1137, 1261
Hpy188III TCNNGA 1 cut(s) 176
Hpy8I GTNNAC 2 cut(s) 559, 1202
HpyAV CCTTC 3 cut(s) 84, 759, 1118
HpyCH4III ACNGT 1 cut(s) 413
HpyCH4V TGCA 6 cut(s) 36, 64, 288, 650, 948, 1068
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 766
HpyF3I CTNAG 4 cut(s) 630, 972, 998, 1120
Hsp92II CATG 4 cut(s) 948, 982, 1223, 1236
HspAI GCGC 1 cut(s) 39
Kzo9I GATC 6 cut(s) 166, 178, 217, 434, 692, 790
LmnI GCTCC 1 cut(s) 1157
Lsp1109I GCAGC 3 cut(s) 48, 492, 810
LweI GCATC 1 cut(s) 637
MaeI CTAG 4 cut(s) 500, 509, 578, 788
MaeIII GTNAC 5 cut(s) 476, 519, 682, 828, 1181
MalI GATC 6 cut(s) 168, 180, 219, 436, 694, 792
MboI GATC 6 cut(s) 166, 178, 217, 434, 692, 790
MboII GAAGA 5 cut(s) 19, 272, 343, 633, 848
MfeI CAATTG 3 cut(s) 355, 857, 879
MflI RGATCY 3 cut(s) 178, 692, 790
MhlI GDGCHC 2 cut(s) 966, 1078
MlyI GAGTC 1 cut(s) 187
MmeI TCCRAC 1 cut(s) 894
MnlI CCTC 7 cut(s) 374, 558, 733, 953, 1140, 1227, 1267
MseI TTAA 7 cut(s) 335, 389, 483, 722, 956, 1014, 1113
MspCI CTTAAG 1 cut(s) 721
MspI CCGG 1 cut(s) 582
MspR9I CCNGG 2 cut(s) 444, 1187
MunI CAATTG 3 cut(s) 355, 857, 879
MvaI CCWGG 2 cut(s) 444, 1187
MwoI GCNNNNNNNGC 2 cut(s) 61, 766
NcoI CCATGG 1 cut(s) 978
NdeII GATC 6 cut(s) 166, 178, 217, 434, 692, 790
NlaIII CATG 4 cut(s) 948, 982, 1223, 1236
NmuCI GTSAC 2 cut(s) 682, 828
PfeI GAWTC 6 cut(s) 419, 604, 775, 1055, 1174, 1193
PkrI GCNGC 4 cut(s) 38, 507, 570, 800
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
Psp6I CCWGG 2 cut(s) 442, 1185
PspEI GGTNACC 1 cut(s) 476
PspGI CCWGG 2 cut(s) 442, 1185
PspPI GGNCC 2 cut(s) 260, 937
PsuI RGATCY 3 cut(s) 178, 692, 790
RsaI GTAC 2 cut(s) 224, 1036
RsaNI GTAC 2 cut(s) 223, 1035
SaqAI TTAA 7 cut(s) 335, 389, 483, 722, 956, 1014, 1113
SatI GCNGC 4 cut(s) 37, 506, 569, 799
Sau3AI GATC 6 cut(s) 166, 178, 217, 434, 692, 790
Sau96I GGNCC 2 cut(s) 260, 937
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 2 cut(s) 444, 1187
SduI GDGCHC 2 cut(s) 966, 1078
SfaNI GCATC 1 cut(s) 637
SfcI CTRYAG 1 cut(s) 753
SinI GGWCC 2 cut(s) 260, 937
SmlI CTYRAG 3 cut(s) 326, 562, 721
SmoI CTYRAG 3 cut(s) 326, 562, 721
SsiI CCGC 2 cut(s) 263, 568
SspMI CTAG 4 cut(s) 500, 509, 578, 788
StyD4I CCNGG 2 cut(s) 442, 1185
StyI CCWWGG 4 cut(s) 133, 423, 978, 1046
TaaI ACNGT 1 cut(s) 413
TaqI TCGA 2 cut(s) 191, 433
TatI WGTACW 1 cut(s) 222
TauI GCSGC 1 cut(s) 571
TfiI GAWTC 6 cut(s) 419, 604, 775, 1055, 1174, 1193
Tru1I TTAA 7 cut(s) 335, 389, 483, 722, 956, 1014, 1113
Tru9I TTAA 7 cut(s) 335, 389, 483, 722, 956, 1014, 1113
TscAI CASTG 2 cut(s) 290, 911
TseFI GTSAC 2 cut(s) 682, 828
TseI GCWGC 3 cut(s) 36, 505, 798
Tsp45I GTSAC 2 cut(s) 682, 828
TspDTI ATGAA 4 cut(s) 17, 663, 933, 1004
TspRI CASTG 2 cut(s) 290, 911
Vha464I CTTAAG 1 cut(s) 721
VpaK11BI GGWCC 2 cut(s) 260, 937
XapI RAATTY 7 cut(s) 428, 459, 807, 900, 930, 1115, 1273
XspI CTAG 4 cut(s) 500, 509, 578, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.