AT3G25520

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001819
Physical Location & Seq
Reverse (-)
9269277 .. 9271465
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25520.1

Sequence Viewer

Length: 906 bp
ATGGTGTTTGTGAAGTCCACCAAGTCGAATGCTTACTTCAAGAGGTACCAAGTGAAGTTCAGGAGAAGAAGAGATGGAAAGACTGACTACAGGGCAAGGATCCGTCTTATCAACCAAGACAAGAACAAGTACAATACTCCTAAGTATCGTTTTGTTGTCCGGTTTACCAACAAAGACATAGTGGCACAGATTGTATCTGCAAGCATTGCTGGTGACATTGTTAAAGCTTCTGCTTACGCACATGAACTGCCTCAGTATGGTCTCACTGTTGGTCTTACAAACTATGCTGCAGCTTACTGTACTGGCCTTCTTTTGGCTCGCCGTGTTTTAAAGATGTTGGAAATGGATGACGAGTATGAGGGAAACGTTGAGGCCACTGGAGAAGACTTTTCCGTTGAGCCAACTGATTCAAGGAGACCTTTCCGTGCTCTTCTTGATGTTGGACTTATCAGGACCACAACAGGAAACCGTGTGTTCGGTGCTCTTAAGGGTGCTTTGGATGGTGGTCTTGATATCCCTCACAGTGACAAGAGATTTGCTGGGTTCCACAAGGAGAACAAGCAACTTGATGCTGAAATCCACAGGAACTACATCTATGGTGGCCATGTCTCAAACTACATGAAGCTGTTGGGAGAAGATGAGCCAGAGAAGTTACAAACTCACTTCAGTGCTTACATCAAGAAAGGAGTTGAAGCTGAGAGCATTGAGGAGTTGTACAAGAAGGTTCACGCAGCTATTCGTGCTGACCCCAACCCAAAGAAAACCGTGAAACCTGCTCCCAAGCAACACAAGAGGTACAACTTGAAGAAACTTACTTACGAGGAGAGGAAGAACAAGTTGATCGAGAGAGTCAAGGCATTGAATGGAGCAGGTGGTGATGATGATGATGAGGACGATGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

301

Amino Acids

34.36

Weight (kDa)

9.32

Isoelectric Point (pI)

38.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L5e PF17144 14 - 175 1.8e-80 Ribosomal large subunit proteins 60S L5, and 50S L18
Ribosomal_L18_c PF14204 235 - 288 5.1e-19 Ribosomal L18 C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 860
Acc36I ACCTGC 2 cut(s) 781, 860
Acc65I GGTACC 1 cut(s) 45
AccB1I GGYRCC 1 cut(s) 45
AclI AACGTT 1 cut(s) 366
AclWI GGATC 2 cut(s) 94, 107
AcoI YGGCCR 1 cut(s) 601
AcuI CTGAAG 1 cut(s) 649
AfaI GTAC 5 cut(s) 47, 131, 301, 716, 797
AfiI CCNNNNNNNGG 2 cut(s) 257, 313
AflII CTTAAG 1 cut(s) 485
AgsI TTSAA 5 cut(s) 40, 411, 692, 805, 862
AleI CACNNNNGTG 1 cut(s) 666
AluBI AGCT 5 cut(s) 227, 293, 625, 695, 734
AluI AGCT 5 cut(s) 227, 293, 625, 695, 734
Alw21I GWGCWC 2 cut(s) 430, 484
Alw26I GTCTC 3 cut(s) 266, 409, 613
AlwI GGATC 2 cut(s) 94, 107
AoxI GGCC 3 cut(s) 304, 372, 601
ApeKI GCWGC 3 cut(s) 287, 290, 731
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
Asp718I GGTACC 1 cut(s) 45
AspS9I GGNCC 1 cut(s) 453
AsuHPI GGTGA 2 cut(s) 224, 887
AvaII GGWCC 1 cut(s) 453
BalI TGGCCA 1 cut(s) 603
BamHI GGATCC 1 cut(s) 99
BanI GGYRCC 1 cut(s) 45
BbsI GAAGAC 1 cut(s) 390
Bbv12I GWGCWC 2 cut(s) 430, 484
BbvI GCAGC 3 cut(s) 274, 302, 743
BccI CCATC 2 cut(s) 68, 494
BceAI ACGGC 1 cut(s) 306
BcoDI GTCTC 3 cut(s) 266, 409, 613
BfmI CTRYAG 2 cut(s) 88, 288
BfrI CTTAAG 1 cut(s) 485
BfuAI ACCTGC 2 cut(s) 781, 860
BisI GCNGC 3 cut(s) 288, 291, 732
BlsI GCNGC 3 cut(s) 289, 292, 733
Bme18I GGWCC 1 cut(s) 453
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 3 cut(s) 47, 101, 545
BmsI GCATC 1 cut(s) 559
BpiI GAAGAC 1 cut(s) 390
BpmI CTGGAG 1 cut(s) 399
BsaI GGTCTC 2 cut(s) 266, 409
BsaWI WCCGGW 1 cut(s) 159
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 313
Bse1I ACTGG 2 cut(s) 307, 382
Bse3DI GCAATG 1 cut(s) 204
BseGI GGATG 2 cut(s) 352, 505
BseLI CCNNNNNNNGG 2 cut(s) 257, 313
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 2 cut(s) 266, 687
BseNI ACTGG 2 cut(s) 307, 382
BseRI GAGGAG 2 cut(s) 722, 836
BseXI GCAGC 3 cut(s) 274, 302, 743
BseYI CCCAGC 1 cut(s) 539
BshFI GGCC 3 cut(s) 306, 374, 603
BshNI GGYRCC 1 cut(s) 45
BsiHKAI GWGCWC 2 cut(s) 430, 484
BsiSI CCGG 1 cut(s) 160
BslI CCNNNNNNNGG 2 cut(s) 257, 313
BsmAI GTCTC 3 cut(s) 266, 409, 613
BsmI GAATGC 1 cut(s) 34
BsnI GGCC 3 cut(s) 306, 374, 603
Bso31I GGTCTC 2 cut(s) 266, 409
Bsp1286I GDGCHC 2 cut(s) 430, 484
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 2 cut(s) 99, 840
BspANI GGCC 3 cut(s) 306, 374, 603
BspCNI CTCAG 2 cut(s) 265, 688
BspLI GGNNCC 3 cut(s) 47, 101, 545
BspMAI CTGCAG 1 cut(s) 292
BspMI ACCTGC 2 cut(s) 781, 860
BspPI GGATC 2 cut(s) 94, 107
BspQI GCTCTTC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 45
BspTI CTTAAG 1 cut(s) 485
BspTNI GGTCTC 2 cut(s) 266, 409
BsrDI GCAATG 1 cut(s) 204
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 2 cut(s) 307, 382
BssMI GATC 2 cut(s) 99, 840
Bst4CI ACNGT 5 cut(s) 268, 299, 470, 524, 766
Bst6I CTCTTC 3 cut(s) 64, 435, 894
BstAFI CTTAAG 1 cut(s) 485
BstAPI GCANNNNNTGC 1 cut(s) 206
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 2 cut(s) 202, 319
BstDEI CTNAG 3 cut(s) 141, 252, 696
BstF5I GGATG 2 cut(s) 352, 505
BstKTI GATC 2 cut(s) 102, 843
BstMAI GTCTC 3 cut(s) 266, 409, 613
BstMBI GATC 2 cut(s) 99, 840
BstMWI GCNNNNNNNGC 2 cut(s) 206, 740
BstSFI CTRYAG 2 cut(s) 88, 288
BstV1I GCAGC 3 cut(s) 274, 302, 743
BstV2I GAAGAC 1 cut(s) 390
BstX2I RGATCY 1 cut(s) 99
BstYI RGATCY 1 cut(s) 99
BsuRI GGCC 3 cut(s) 306, 374, 603
BtsCI GGATG 2 cut(s) 352, 505
BtsIMutI CAGTG 4 cut(s) 264, 375, 529, 673
BveI ACCTGC 2 cut(s) 781, 860
Cac8I GCNNGC 2 cut(s) 202, 319
Cfr13I GGNCC 1 cut(s) 453
Csp6I GTAC 5 cut(s) 46, 130, 300, 715, 796
CviAII CATG 3 cut(s) 242, 605, 619
CviQI GTAC 5 cut(s) 46, 130, 300, 715, 796
DdeI CTNAG 3 cut(s) 141, 252, 696
DpnI GATC 2 cut(s) 101, 842
DpnII GATC 2 cut(s) 99, 840
DraI TTTAAA 1 cut(s) 330
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 3 cut(s) 64, 435, 894
EarI CTCTTC 3 cut(s) 64, 435, 894
Eco31I GGTCTC 2 cut(s) 266, 409
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 453
Eco57I CTGAAG 1 cut(s) 649
EcoRV GATATC 1 cut(s) 514
FaeI CATG 3 cut(s) 245, 608, 622
FaiI YATR 8 cut(s) 179, 243, 258, 285, 357, 597, 606, 620
FalI AAGNNNNNCTT 2 cut(s) 403, 435
FatI CATG 3 cut(s) 241, 604, 618
Fnu4HI GCNGC 3 cut(s) 288, 291, 732
FokI GGATG 2 cut(s) 359, 512
Fsp4HI GCNGC 3 cut(s) 288, 291, 732
GluI GCNGC 3 cut(s) 288, 291, 732
GsaI CCCAGC 1 cut(s) 543
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 3 cut(s) 306, 374, 603
HapII CCGG 1 cut(s) 160
Hin1II CATG 3 cut(s) 245, 608, 622
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 2 cut(s) 407, 849
HpaII CCGG 1 cut(s) 160
HphI GGTGA 2 cut(s) 224, 887
Hpy166II GTNNAC 3 cut(s) 18, 165, 727
Hpy188III TCNNGA 7 cut(s) 40, 61, 434, 451, 509, 679, 844
Hpy8I GTNNAC 3 cut(s) 18, 165, 727
HpyAV CCTTC 2 cut(s) 317, 715
HpyCH4III ACNGT 5 cut(s) 268, 299, 470, 524, 766
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 2 cut(s) 200, 290
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 740
HpyF3I CTNAG 3 cut(s) 141, 252, 696
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 3 cut(s) 245, 608, 622
KpnI GGTACC 1 cut(s) 49
Kzo9I GATC 2 cut(s) 99, 840
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 2 cut(s) 781, 866
Lsp1109I GCAGC 3 cut(s) 274, 302, 743
LweI GCATC 1 cut(s) 559
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 3 cut(s) 212, 524, 651
MalI GATC 2 cut(s) 101, 842
MboI GATC 2 cut(s) 99, 840
MboII GAAGA 7 cut(s) 78, 81, 395, 422, 647, 817, 841
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 2 cut(s) 430, 484
MlsI TGGCCA 1 cut(s) 603
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 1 cut(s) 858
MmeI TCCRAC 2 cut(s) 318, 421
Mox20I TGGCCA 1 cut(s) 603
MscI TGGCCA 1 cut(s) 603
MseI TTAA 3 cut(s) 222, 329, 486
MslI CAYNNNNRTG 1 cut(s) 666
Msp20I TGGCCA 1 cut(s) 603
MspCI CTTAAG 1 cut(s) 485
MspI CCGG 1 cut(s) 160
Mva1269I GAATGC 1 cut(s) 34
MwoI GCNNNNNNNGC 2 cut(s) 206, 740
NdeII GATC 2 cut(s) 99, 840
NlaIII CATG 3 cut(s) 245, 608, 622
NlaIV GGNNCC 3 cut(s) 47, 101, 545
NmuCI GTSAC 2 cut(s) 212, 524
OliI CACNNNNGTG 1 cut(s) 666
PaqCI CACCTGC 1 cut(s) 860
PciSI GCTCTTC 1 cut(s) 435
PctI GAATGC 1 cut(s) 34
PfeI GAWTC 1 cut(s) 407
PkrI GCNGC 3 cut(s) 289, 292, 733
PleI GAGTC 1 cut(s) 857
PpsI GAGTC 1 cut(s) 857
Psp1406I AACGTT 1 cut(s) 366
PspFI CCCAGC 1 cut(s) 539
PspN4I GGNNCC 3 cut(s) 47, 101, 545
PspPI GGNCC 1 cut(s) 453
PstI CTGCAG 1 cut(s) 292
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 5 cut(s) 47, 131, 301, 716, 797
RsaNI GTAC 5 cut(s) 46, 130, 300, 715, 796
RseI CAYNNNNRTG 1 cut(s) 666
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 3 cut(s) 222, 329, 486
SatI GCNGC 3 cut(s) 288, 291, 732
Sau3AI GATC 2 cut(s) 99, 840
Sau96I GGNCC 1 cut(s) 453
SchI GAGTC 1 cut(s) 858
SduI GDGCHC 2 cut(s) 430, 484
SfaNI GCATC 1 cut(s) 559
SfcI CTRYAG 2 cut(s) 88, 288
SinI GGWCC 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
TaaI ACNGT 5 cut(s) 268, 299, 470, 524, 766
TaiI ACGT 1 cut(s) 369
TaqI TCGA 2 cut(s) 26, 843
TatI WGTACW 3 cut(s) 129, 299, 714
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 3 cut(s) 222, 329, 486
Tru9I TTAA 3 cut(s) 222, 329, 486
TscAI CASTG 4 cut(s) 271, 382, 529, 673
TseFI GTSAC 2 cut(s) 212, 524
TseI GCWGC 3 cut(s) 287, 290, 731
Tsp45I GTSAC 2 cut(s) 212, 524
TspDTI ATGAA 2 cut(s) 258, 635
TspGWI ACGGA 3 cut(s) 92, 382, 413
TspRI CASTG 4 cut(s) 271, 382, 529, 673
Vha464I CTTAAG 1 cut(s) 485
VpaK11BI GGWCC 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.