MD15G1422600.v1.1

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001819
Physical Location & Seq
Reverse (-)
52426595 .. 52429476
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1422600.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGTTTTCGCCAAAGCTCAGAAATCAAGGGCTTACTTCAAGAGATTTCAAGTCAAGTACAGGAGAAGGCGAGAGGGAAAGACCGACTACCGTGCCCGGATTAGGCTCATTAACCAAGACAAGAACAAGTACAACACACCCAAGTATCGGTTTGTTGTGAGATTTACCAACAAGGATATTGTTGCTCAGATTATATCTGCCAGTATTGCTGGTGATTTGGTTCTTGCTGCAGCCTACTCCCATGAGCTCCCACGCTATGGGCTTGAAGTTGGTCTCACAAACTATGCCGCAGCCTACTGCACTGGCCTACTCTTGGCTCGTCGTGTGTTGAAGAAGCTTGAAATGGATGATGAGTACGAGGGAAATGTTGAGGCTACTGGTGAAGACTATTCTGTAGAGCCAGCTGAGAGCAGAAGGCCATTCCGTGCTCTTCTTGATGTTGGCCTGATCAGGACCACCACAGGAAACCGTGTATTCGGTGCCTTGAAGGGTGCTTTGGATGGGGGTTTGGATATCCCTCACAGTGAAAAGAGGTTTGCCGGTTTCTCCAAGGATGGTAAGCAACTTGACGCTGAGATTCATCGCAAGTACATTTATGGTGGCCATGTTGCTGCATACATGAGGACTCTGGCGGAGGATGAGCCAGAGAAATACCAGAGTCACTTCAGTGAATATATTAAGAAAGGCATTGAAGCGGATGACATCGAGGAGGTGTACAAGAAAGTTCATGCTGCCATCCGTGCTGATCCTACAATTAAGAAGACTGAAAAGCCGGCTCCTCAGGAGCACAAGAGGTACAACTTGAAGAAGCTTACCTTTGATGAGAGGAAGAAGAAGTTGGTTGACAGGTTGAAGGCCTTCAATGATGCTGGTGACGATGACGACAGTGATGATGACGACGAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

303

Amino Acids

34.7

Weight (kDa)

9.09

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L5e PF17144 14 - 175 7e-83 Ribosomal large subunit proteins 60S L5, and 50S L18
Ribosomal_L18_c PF14204 235 - 288 5.5e-18 Ribosomal L18 C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 479
AccB7I CCANNNNNTGG 1 cut(s) 257
AciI CCGC 3 cut(s) 288, 632, 695
AclWI GGATC 1 cut(s) 740
AcoI YGGCCR 1 cut(s) 601
AcuI CTGAAG 1 cut(s) 649
AfaI GTAC 7 cut(s) 59, 131, 356, 590, 716, 797, 905
AfiI CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
AjuI GAANNNNNNNTTGG 4 cut(s) 479, 511, 821, 853
AleI CACNNNNGTG 1 cut(s) 666
AluBI AGCT 5 cut(s) 17, 247, 337, 404, 811
AluI AGCT 5 cut(s) 17, 247, 337, 404, 811
Alw21I GWGCWC 3 cut(s) 249, 430, 789
Alw26I GTCTC 1 cut(s) 278
AlwI GGATC 1 cut(s) 740
AoxI GGCC 5 cut(s) 304, 416, 442, 601, 855
ApeKI GCWGC 5 cut(s) 227, 230, 290, 611, 731
Asp700I GAANNNNTTC 1 cut(s) 857
AspS9I GGNCC 1 cut(s) 453
AsuC2I CCSGG 1 cut(s) 97
AsuHPI GGTGA 3 cut(s) 224, 392, 884
AvaII GGWCC 1 cut(s) 453
AxyI CCTNAGG 1 cut(s) 780
BaeGI GKGCMC 1 cut(s) 97
BaeI ACNNNNGTAYC 2 cut(s) 128, 161
BalI TGGCCA 1 cut(s) 603
BanI GGYRCC 1 cut(s) 479
BanII GRGCYC 1 cut(s) 249
BbsI GAAGAC 2 cut(s) 390, 767
Bbv12I GWGCWC 3 cut(s) 249, 430, 789
BbvI GCAGC 5 cut(s) 214, 242, 302, 598, 718
BccI CCATC 3 cut(s) 494, 548, 743
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BclI TGATCA 1 cut(s) 447
BcnI CCSGG 1 cut(s) 97
BcoDI GTCTC 1 cut(s) 278
BfmI CTRYAG 2 cut(s) 228, 393
BisI GCNGC 6 cut(s) 228, 231, 288, 291, 612, 732
BlsI GCNGC 6 cut(s) 229, 232, 289, 292, 613, 733
BmcAI AGTACT 1 cut(s) 905
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 453
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 2 cut(s) 481, 777
BmrFI CCNGG 1 cut(s) 97
BmsI GCATC 1 cut(s) 856
BpiI GAAGAC 2 cut(s) 390, 767
BpuMI CCSGG 1 cut(s) 97
BsaI GGTCTC 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 549
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
Bse118I RCCGGY 2 cut(s) 539, 772
Bse1I ACTGG 3 cut(s) 201, 307, 382
Bse21I CCTNAGG 1 cut(s) 780
BseDI CCNNGG 1 cut(s) 549
BseGI GGATG 6 cut(s) 352, 505, 559, 643, 703, 735
BseLI CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
BseMII CTCAG 5 cut(s) 32, 200, 396, 564, 794
BseNI ACTGG 3 cut(s) 201, 307, 382
BseRI GAGGAG 2 cut(s) 722, 768
BseSI GKGCMC 1 cut(s) 97
BseXI GCAGC 5 cut(s) 214, 242, 302, 598, 718
BsgI GTGCAG 1 cut(s) 283
BshFI GGCC 5 cut(s) 306, 418, 444, 603, 857
BshNI GGYRCC 1 cut(s) 479
BsiHKAI GWGCWC 3 cut(s) 249, 430, 789
BsiSI CCGG 3 cut(s) 97, 540, 773
BslI CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
BsmAI GTCTC 1 cut(s) 278
BsnI GGCC 5 cut(s) 306, 418, 444, 603, 857
Bso31I GGTCTC 1 cut(s) 278
Bsp1286I GDGCHC 4 cut(s) 97, 249, 430, 789
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 2 cut(s) 447, 745
BspACI CCGC 3 cut(s) 288, 632, 695
BspANI GGCC 5 cut(s) 306, 418, 444, 603, 857
BspCNI CTCAG 5 cut(s) 31, 199, 397, 565, 793
BspLI GGNNCC 2 cut(s) 481, 777
BspMAI CTGCAG 1 cut(s) 232
BspPI GGATC 1 cut(s) 740
BspQI GCTCTTC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 479
BspTNI GGTCTC 1 cut(s) 278
BsrFI RCCGGY 2 cut(s) 539, 772
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 3 cut(s) 201, 307, 382
BssAI RCCGGY 2 cut(s) 539, 772
BssECI CCNNGG 1 cut(s) 549
BssMI GATC 2 cut(s) 447, 745
BssT1I CCWWGG 1 cut(s) 549
Bst4CI ACNGT 4 cut(s) 92, 470, 524, 887
Bst6I CTCTTC 1 cut(s) 435
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 2 cut(s) 402, 774
BstDEI CTNAG 5 cut(s) 18, 186, 405, 573, 780
BstF5I GGATG 6 cut(s) 352, 505, 559, 643, 703, 735
BstKTI GATC 2 cut(s) 450, 748
BstMAI GTCTC 1 cut(s) 278
BstMBI GATC 2 cut(s) 447, 745
BstMWI GCNNNNNNNGC 2 cut(s) 206, 740
BstSCI CCNGG 1 cut(s) 95
BstSFI CTRYAG 2 cut(s) 228, 393
BstSLI GKGCMC 1 cut(s) 97
BstV1I GCAGC 5 cut(s) 214, 242, 302, 598, 718
BstV2I GAAGAC 2 cut(s) 390, 767
Bsu36I CCTNAGG 1 cut(s) 780
BsuRI GGCC 5 cut(s) 306, 418, 444, 603, 857
BtgZI GCGATG 1 cut(s) 566
BtsCI GGATG 6 cut(s) 352, 505, 559, 643, 703, 735
BtsIMutI CAGTG 4 cut(s) 300, 529, 673, 892
Cac8I GCNNGC 2 cut(s) 402, 774
Cfr10I RCCGGY 2 cut(s) 539, 772
Cfr13I GGNCC 1 cut(s) 453
CseI GACGC 1 cut(s) 578
Csp6I GTAC 7 cut(s) 58, 130, 355, 589, 715, 796, 904
CviAII CATG 4 cut(s) 242, 605, 619, 728
CviQI GTAC 7 cut(s) 58, 130, 355, 589, 715, 796, 904
DdeI CTNAG 5 cut(s) 18, 186, 405, 573, 780
DpnI GATC 2 cut(s) 449, 747
DpnII GATC 2 cut(s) 447, 745
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
EciI GGCGGA 1 cut(s) 647
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 549
Eco147I AGGCCT 1 cut(s) 857
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 278
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 453
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 649
Eco81I CCTNAGG 1 cut(s) 780
EcoICRI GAGCTC 1 cut(s) 247
EcoRV GATATC 1 cut(s) 514
EcoT14I CCWWGG 1 cut(s) 549
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 549
FaeI CATG 4 cut(s) 245, 608, 622, 731
FalI AAGNNNNNCTT 2 cut(s) 800, 832
FatI CATG 4 cut(s) 241, 604, 618, 727
FbaI TGATCA 1 cut(s) 447
Fnu4HI GCNGC 6 cut(s) 228, 231, 288, 291, 612, 732
FokI GGATG 6 cut(s) 359, 512, 566, 650, 710, 722
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 6 cut(s) 228, 231, 288, 291, 612, 732
GluI GCNGC 6 cut(s) 228, 231, 288, 291, 612, 732
HaeIII GGCC 5 cut(s) 306, 418, 444, 603, 857
HapII CCGG 3 cut(s) 97, 540, 773
HgaI GACGC 1 cut(s) 578
Hin1II CATG 4 cut(s) 245, 608, 622, 731
HincII GTYRAC 1 cut(s) 844
HindII GTYRAC 1 cut(s) 844
HindIII AAGCTT 2 cut(s) 335, 809
HinfI GANTC 3 cut(s) 577, 625, 658
HpaII CCGG 3 cut(s) 97, 540, 773
HphI GGTGA 3 cut(s) 224, 392, 884
Hpy166II GTNNAC 2 cut(s) 715, 844
Hpy188I TCNGA 2 cut(s) 21, 189
Hpy188III TCNNGA 4 cut(s) 40, 434, 451, 782
Hpy8I GTNNAC 2 cut(s) 715, 844
Hpy99I CGWCG 2 cut(s) 324, 902
HpyAV CCTTC 5 cut(s) 60, 408, 481, 847, 868
HpyCH4III ACNGT 4 cut(s) 92, 470, 524, 887
HpyCH4V TGCA 3 cut(s) 230, 300, 614
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 740
HpyF3I CTNAG 5 cut(s) 18, 186, 405, 573, 780
Hsp92II CATG 4 cut(s) 245, 608, 622, 731
KroI GCCGGC 1 cut(s) 772
KroNI GCCGGC 1 cut(s) 774
Ksp22I TGATCA 1 cut(s) 447
Kzo9I GATC 2 cut(s) 447, 745
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 3 cut(s) 252, 781, 784
Lsp1109I GCAGC 5 cut(s) 214, 242, 302, 598, 718
LweI GCATC 1 cut(s) 856
MaeIII GTNAC 2 cut(s) 659, 872
MalI GATC 2 cut(s) 449, 747
MboI GATC 2 cut(s) 447, 745
MboII GAAGA 7 cut(s) 343, 395, 422, 772, 817, 841, 844
MhlI GDGCHC 4 cut(s) 97, 249, 430, 789
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 1 cut(s) 753
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 2 cut(s) 619, 667
Mox20I TGGCCA 1 cut(s) 603
MroNI GCCGGC 1 cut(s) 772
MroXI GAANNNNTTC 1 cut(s) 857
MscI TGGCCA 1 cut(s) 603
MseI TTAA 3 cut(s) 111, 678, 756
MslI CAYNNNNRTG 1 cut(s) 666
Msp20I TGGCCA 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 3 cut(s) 97, 540, 773
MspR9I CCNGG 1 cut(s) 97
MwoI GCNNNNNNNGC 2 cut(s) 206, 740
NaeI GCCGGC 1 cut(s) 774
NciI CCSGG 1 cut(s) 97
NdeII GATC 2 cut(s) 447, 745
NgoMIV GCCGGC 1 cut(s) 772
NlaIII CATG 4 cut(s) 245, 608, 622, 731
NlaIV GGNNCC 2 cut(s) 481, 777
NmuCI GTSAC 2 cut(s) 659, 872
OliI CACNNNNGTG 1 cut(s) 666
PceI AGGCCT 1 cut(s) 857
PciSI GCTCTTC 1 cut(s) 435
PdiI GCCGGC 1 cut(s) 774
PdmI GAANNNNTTC 1 cut(s) 857
PfeI GAWTC 1 cut(s) 577
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 6 cut(s) 229, 232, 289, 292, 613, 733
PleI GAGTC 2 cut(s) 619, 666
PpsI GAGTC 2 cut(s) 619, 666
Psp124BI GAGCTC 1 cut(s) 249
PspN4I GGNNCC 2 cut(s) 481, 777
PspPI GGNCC 1 cut(s) 453
PstI CTGCAG 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 7 cut(s) 59, 131, 356, 590, 716, 797, 905
RsaNI GTAC 7 cut(s) 58, 130, 355, 589, 715, 796, 904
RseI CAYNNNNRTG 1 cut(s) 666
SacI GAGCTC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 3 cut(s) 111, 678, 756
SatI GCNGC 6 cut(s) 228, 231, 288, 291, 612, 732
Sau3AI GATC 2 cut(s) 447, 745
Sau96I GGNCC 1 cut(s) 453
ScaI AGTACT 1 cut(s) 905
SchI GAGTC 2 cut(s) 619, 667
ScrFI CCNGG 1 cut(s) 97
SduI GDGCHC 4 cut(s) 97, 249, 430, 789
SfaNI GCATC 1 cut(s) 856
SfcI CTRYAG 2 cut(s) 228, 393
SinI GGWCC 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 666
Sse9I AATT 1 cut(s) 753
SseBI AGGCCT 1 cut(s) 857
SsiI CCGC 3 cut(s) 288, 632, 695
SstI GAGCTC 1 cut(s) 249
StuI AGGCCT 1 cut(s) 857
StyD4I CCNGG 1 cut(s) 95
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 4 cut(s) 92, 470, 524, 887
TaqI TCGA 1 cut(s) 705
TaqII GACCGA 1 cut(s) 98
TasI AATT 1 cut(s) 753
TatI WGTACW 5 cut(s) 57, 129, 588, 714, 903
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 1 cut(s) 577
Tru1I TTAA 3 cut(s) 111, 678, 756
Tru9I TTAA 3 cut(s) 111, 678, 756
TscAI CASTG 4 cut(s) 307, 529, 673, 892
TseFI GTSAC 2 cut(s) 659, 872
TseI GCWGC 5 cut(s) 227, 230, 290, 611, 731
Tsp45I GTSAC 2 cut(s) 659, 872
TspDTI ATGAA 2 cut(s) 569, 716
TspGWI ACGGA 2 cut(s) 413, 728
TspRI CASTG 4 cut(s) 307, 529, 673, 892
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 857
ZrmI AGTACT 1 cut(s) 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.