AT5G39740

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001819
Physical Location & Seq
Forward (+)
15903279 .. 15905532
2254 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G39740.2

Sequence Viewer

Length: 906 bp
ATGGTGTTTGTGAAGTCCTCCAAATCGAATGCTTACTTCAAGAGGTACCAAGTGAAGTTCAGGAGAAGGAGAGATGGGAAGACTGATTACAGGGCAAGGATCCGTCTTATCAATCAAGACAAGAACAAGTACAACACCCCTAAGTACCGTTTTGTTGTTCGATTTACCAACAAAGACATTGTGGCACAGATTGTATCTGCAAGCATAGCTGGTGACATTGTTAAAGCTTCGGCTTACGCACATGAGCTTCCTCAGTATGGACTCACTGTTGGTCTTACCAACTATGCTGCAGCTTACTGTACTGGCCTTCTTTTGGCTCGTCGTGTTCTGAAAATGCTGGAAATGGATGATGAGTACGAGGGAAACGTAGAGGCCACTGGAGAGGACTTTTCTGTGGAACCAACTGATTCCAGAAGACCTTTCCGTGCTCTTCTTGATGTTGGACTTATCAGGACTACCACTGGAAACCGTGTGTTTGGTGCACTCAAGGGAGCTTTGGACGGAGGTCTTGATATCCCTCACAGTGACAAGAGATTTGCTGGTTTCCACAAAGAGAACAAGCAACTTGATGCTGAAATCCACAGGAACTACATCTACGGTGGCCACGTCTCAAACTACATGAAGCTGTTGGGGGAAGATGAGCCGGAGAAGTTGCAAACTCACTTCAGTGCTTACATTAAGAAAGGAGTTGAAGCTGAGAGCATCGAGGAGATGTACAAGAAGGTTCACGCAGCTATCCGAGCAGAACCCAACCATAAGAAAACCGAGAAATCTGCTCCCAAGGAACACAAGAGGTACAACTTGAAGAAACTGACTTACGAAGAGAGGAAGAACAAGTTGATCGAGAGAGTGAAGGCATTAAACGGAGCAGGTGGTGATGATGATGATGAGGATGATGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

301

Amino Acids

34.44

Weight (kDa)

9.18

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L5e PF17144 14 - 175 1.8e-80 Ribosomal large subunit proteins 60S L5, and 50S L18
Ribosomal_L18_c PF14204 235 - 288 9.5e-20 Ribosomal L18 C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 860
Acc36I ACCTGC 1 cut(s) 860
Acc65I GGTACC 1 cut(s) 45
AccB1I GGYRCC 1 cut(s) 45
AclWI GGATC 2 cut(s) 94, 107
AcoI YGGCCR 1 cut(s) 601
AcuI CTGAAG 1 cut(s) 649
AfaI GTAC 7 cut(s) 47, 131, 146, 301, 356, 716, 797
AfiI CCNNNNNNNGG 2 cut(s) 257, 313
AgsI TTSAA 3 cut(s) 40, 692, 805
AjiI CACGTC 1 cut(s) 607
AleI CACNNNNGTG 1 cut(s) 666
AluBI AGCT 8 cut(s) 209, 227, 247, 293, 494, 625, 695, 734
AluI AGCT 8 cut(s) 209, 227, 247, 293, 494, 625, 695, 734
Alw21I GWGCWC 2 cut(s) 430, 484
Alw26I GTCTC 1 cut(s) 613
Alw44I GTGCAC 1 cut(s) 480
AlwI GGATC 2 cut(s) 94, 107
AoxI GGCC 3 cut(s) 304, 372, 601
ApaLI GTGCAC 1 cut(s) 480
ApeKI GCWGC 3 cut(s) 287, 290, 731
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
Asp718I GGTACC 1 cut(s) 45
AsuHPI GGTGA 2 cut(s) 224, 887
BaeGI GKGCMC 1 cut(s) 484
BalI TGGCCA 1 cut(s) 603
BamHI GGATCC 1 cut(s) 99
BanI GGYRCC 1 cut(s) 45
BarI GAAGNNNNNNTAC 2 cut(s) 71, 103
BbsI GAAGAC 2 cut(s) 86, 421
Bbv12I GWGCWC 2 cut(s) 430, 484
BbvI GCAGC 3 cut(s) 274, 302, 743
BccI CCATC 1 cut(s) 68
BcgI CGANNNNNNTGC 2 cut(s) 755, 789
BcoDI GTCTC 1 cut(s) 613
BfmI CTRYAG 1 cut(s) 288
BfuAI ACCTGC 1 cut(s) 860
BisI GCNGC 3 cut(s) 288, 291, 732
BlsI GCNGC 3 cut(s) 289, 292, 733
BmgBI CACGTC 1 cut(s) 607
BmiI GGNNCC 3 cut(s) 47, 101, 399
BmsI GCATC 2 cut(s) 559, 711
BoxI GACNNNNGTC 1 cut(s) 504
BpiI GAAGAC 2 cut(s) 86, 421
BpmI CTGGAG 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 780
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 313
Bse1I ACTGG 3 cut(s) 307, 382, 466
BseDI CCNNGG 1 cut(s) 780
BseGI GGATG 2 cut(s) 352, 898
BseLI CCNNNNNNNGG 2 cut(s) 257, 313
BseMII CTCAG 2 cut(s) 266, 687
BseNI ACTGG 3 cut(s) 307, 382, 466
BseRI GAGGAG 1 cut(s) 722
BseSI GKGCMC 1 cut(s) 484
BseXI GCAGC 3 cut(s) 274, 302, 743
BshFI GGCC 3 cut(s) 306, 374, 603
BshNI GGYRCC 1 cut(s) 45
BsiHKAI GWGCWC 2 cut(s) 430, 484
BsiSI CCGG 1 cut(s) 644
BslI CCNNNNNNNGG 2 cut(s) 257, 313
BsmAI GTCTC 1 cut(s) 613
BsmBI CGTCTC 1 cut(s) 613
BsmI GAATGC 1 cut(s) 34
BsnI GGCC 3 cut(s) 306, 374, 603
Bsp1286I GDGCHC 2 cut(s) 430, 484
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 2 cut(s) 99, 840
BspANI GGCC 3 cut(s) 306, 374, 603
BspCNI CTCAG 2 cut(s) 265, 688
BspLI GGNNCC 3 cut(s) 47, 101, 399
BspMAI CTGCAG 1 cut(s) 292
BspMI ACCTGC 1 cut(s) 860
BspPI GGATC 2 cut(s) 94, 107
BspQI GCTCTTC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 45
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 3 cut(s) 307, 382, 466
BssECI CCNNGG 1 cut(s) 780
BssMI GATC 2 cut(s) 99, 840
BssT1I CCWWGG 1 cut(s) 780
Bst4CI ACNGT 6 cut(s) 149, 268, 299, 470, 524, 599
Bst6I CTCTTC 3 cut(s) 435, 816, 894
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 3 cut(s) 141, 252, 696
BstF5I GGATG 2 cut(s) 352, 898
BstKTI GATC 2 cut(s) 102, 843
BstMAI GTCTC 1 cut(s) 613
BstMBI GATC 2 cut(s) 99, 840
BstMWI GCNNNNNNNGC 2 cut(s) 206, 740
BstPAI GACNNNNGTC 1 cut(s) 504
BstSFI CTRYAG 1 cut(s) 288
BstSLI GKGCMC 1 cut(s) 484
BstV1I GCAGC 3 cut(s) 274, 302, 743
BstV2I GAAGAC 2 cut(s) 86, 421
BstX2I RGATCY 1 cut(s) 99
BstYI RGATCY 1 cut(s) 99
BsuRI GGCC 3 cut(s) 306, 374, 603
BtrI CACGTC 1 cut(s) 607
BtsCI GGATG 2 cut(s) 352, 898
BtsIMutI CAGTG 5 cut(s) 264, 375, 459, 529, 673
BveI ACCTGC 1 cut(s) 860
Cac8I GCNNGC 1 cut(s) 202
Csp6I GTAC 7 cut(s) 46, 130, 145, 300, 355, 715, 796
CviAII CATG 2 cut(s) 242, 619
CviQI GTAC 7 cut(s) 46, 130, 145, 300, 355, 715, 796
DdeI CTNAG 3 cut(s) 141, 252, 696
DpnI GATC 2 cut(s) 101, 842
DpnII GATC 2 cut(s) 99, 840
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 3 cut(s) 435, 816, 894
EarI CTCTTC 3 cut(s) 435, 816, 894
Eco130I CCWWGG 1 cut(s) 780
Eco32I GATATC 1 cut(s) 514
Eco57I CTGAAG 1 cut(s) 649
EcoRV GATATC 1 cut(s) 514
EcoT14I CCWWGG 1 cut(s) 780
ErhI CCWWGG 1 cut(s) 780
Esp3I CGTCTC 1 cut(s) 613
FaeI CATG 2 cut(s) 245, 622
FaiI YATR 6 cut(s) 206, 243, 258, 285, 620, 756
FatI CATG 2 cut(s) 241, 618
Fnu4HI GCNGC 3 cut(s) 288, 291, 732
FokI GGATG 1 cut(s) 359
Fsp4HI GCNGC 3 cut(s) 288, 291, 732
GluI GCNGC 3 cut(s) 288, 291, 732
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 3 cut(s) 306, 374, 603
HapII CCGG 1 cut(s) 644
Hin1II CATG 2 cut(s) 245, 622
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 2 cut(s) 261, 407
HpaII CCGG 1 cut(s) 644
HphI GGTGA 2 cut(s) 224, 887
Hpy166II GTNNAC 2 cut(s) 482, 727
Hpy188I TCNGA 2 cut(s) 330, 740
Hpy188III TCNNGA 8 cut(s) 40, 61, 116, 411, 434, 451, 509, 844
Hpy8I GTNNAC 2 cut(s) 482, 727
Hpy99I CGWCG 1 cut(s) 324
HpyAV CCTTC 4 cut(s) 60, 317, 715, 847
HpyCH4III ACNGT 6 cut(s) 149, 268, 299, 470, 524, 599
HpyCH4IV ACGT 2 cut(s) 366, 606
HpyCH4V TGCA 4 cut(s) 200, 290, 482, 655
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 740
HpyF3I CTNAG 3 cut(s) 141, 252, 696
HpySE526I ACGT 2 cut(s) 366, 606
Hsp92II CATG 2 cut(s) 245, 622
KpnI GGTACC 1 cut(s) 49
Kzo9I GATC 2 cut(s) 99, 840
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 3 cut(s) 491, 781, 866
Lsp1109I GCAGC 3 cut(s) 274, 302, 743
LweI GCATC 2 cut(s) 559, 711
MaeII ACGT 2 cut(s) 366, 606
MaeIII GTNAC 2 cut(s) 212, 524
MalI GATC 2 cut(s) 101, 842
MboI GATC 2 cut(s) 99, 840
MboII GAAGA 7 cut(s) 91, 422, 426, 647, 817, 833, 841
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 2 cut(s) 430, 484
MlsI TGGCCA 1 cut(s) 603
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 1 cut(s) 255
MmeI TCCRAC 1 cut(s) 421
Mox20I TGGCCA 1 cut(s) 603
MscI TGGCCA 1 cut(s) 603
MseI TTAA 3 cut(s) 222, 678, 860
MslI CAYNNNNRTG 1 cut(s) 666
Msp20I TGGCCA 1 cut(s) 603
MspI CCGG 1 cut(s) 644
Mva1269I GAATGC 1 cut(s) 34
MwoI GCNNNNNNNGC 2 cut(s) 206, 740
NdeII GATC 2 cut(s) 99, 840
NlaIII CATG 2 cut(s) 245, 622
NlaIV GGNNCC 3 cut(s) 47, 101, 399
NmuCI GTSAC 2 cut(s) 212, 524
OliI CACNNNNGTG 1 cut(s) 666
PaqCI CACCTGC 1 cut(s) 860
PciSI GCTCTTC 1 cut(s) 435
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 603
PctI GAATGC 1 cut(s) 34
PfeI GAWTC 1 cut(s) 407
PkrI GCNGC 3 cut(s) 289, 292, 733
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
PshAI GACNNNNGTC 1 cut(s) 504
PspN4I GGNNCC 3 cut(s) 47, 101, 399
PsrI GAACNNNNNNTAC 2 cut(s) 578, 610
PstI CTGCAG 1 cut(s) 292
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 7 cut(s) 47, 131, 146, 301, 356, 716, 797
RsaNI GTAC 7 cut(s) 46, 130, 145, 300, 355, 715, 796
RseI CAYNNNNRTG 1 cut(s) 666
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 3 cut(s) 222, 678, 860
SatI GCNGC 3 cut(s) 288, 291, 732
Sau3AI GATC 2 cut(s) 99, 840
SchI GAGTC 1 cut(s) 255
SduI GDGCHC 2 cut(s) 430, 484
SfaNI GCATC 2 cut(s) 559, 711
SfcI CTRYAG 1 cut(s) 288
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
StyI CCWWGG 1 cut(s) 780
TaaI ACNGT 6 cut(s) 149, 268, 299, 470, 524, 599
TaiI ACGT 2 cut(s) 369, 609
TaqI TCGA 4 cut(s) 26, 160, 705, 843
TatI WGTACW 3 cut(s) 129, 299, 714
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 3 cut(s) 222, 678, 860
Tru9I TTAA 3 cut(s) 222, 678, 860
TscAI CASTG 5 cut(s) 271, 382, 466, 529, 673
TseFI GTSAC 2 cut(s) 212, 524
TseI GCWGC 3 cut(s) 287, 290, 731
Tsp45I GTSAC 2 cut(s) 212, 524
TspDTI ATGAA 1 cut(s) 635
TspGWI ACGGA 4 cut(s) 92, 413, 516, 879
TspRI CASTG 5 cut(s) 271, 382, 466, 529, 673
VneI GTGCAC 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.