RLG00000015659

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001819
Physical Location & Seq
Reverse (-)
470456 .. 473019
2564 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015659

Sequence Viewer

Length: 783 bp
ATGGCTTTTGCCAAGGCTCAAAAGTCCAAGGCCTACTTCAAGAGGTTCCAAGTCAAGTACAAGAGAAGGCGAGAGGGTAAGACTGACTACCGGGCAAGAATCAGGCTCATCAATCAAGACAAGAATAAATACAACACCCCCAAGTACCGGTTTGTTGTGAGATTTACAAACAAGGATATTGTTGCTCAGATCATATCTGCCAGCCTTGCTGGTGATTTGGTCCTCGCATCAGCCTATGCTCACGAGCTGCCACACTATGGGCTTGAAGTTGGTCTCACAAACTATGCTGCAGCTTACTGCACTGGGCTTCTCTTGGCTCGCCGTGTCTTGAAGACACTTGAGATGGATGCTGAGTATGAGGGCAATGTTGAGGGAGCTTTGGACGGTGGTTTGGATATTCCACACAGTGAAAAGAGGTTTGCTGGTTTCTCCAAGGACAACAAGCAGCTTGATGCTGAGGTCCATCGCAAGTATATCTATGGTGGCCATGTTGCTGCATACATGAGGACTTTGATGGAGGATGAACCAGAAAAGTATCAGAGCCATTTCAGTGAATACATTAAGAAAGGAATAGAAGCAGATAACATCGAAGAGATTTACAAGAAAGTGCATGCAGCCATCAGGGCCGACCCTACAATGAAGAAATCTGAAAAGCCTCAACGCTCGGAACACAAGAGGTTTAACCTCAAGAAGCTTACCTACGAGGAAAGGAAGAACAAATTGATTGAGAGACTGAATGCCTTCAACTCAGCCGCCCATGCTGATGACGACGATGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

261

Amino Acids

30.08

Weight (kDa)

9.28

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L5e PF17144 14 - 124 1.8e-54 Ribosomal large subunit proteins 60S L5, and 50S L18
Ribosomal_L18_c PF14204 196 - 249 2.3e-19 Ribosomal L18 C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 257
AciI CCGC 1 cut(s) 753
AcoI YGGCCR 1 cut(s) 484
AdeI CACNNNGTG 1 cut(s) 407
AfaI GTAC 2 cut(s) 59, 146
AfiI CCNNNNNNNGG 2 cut(s) 147, 257
AgeI ACCGGT 1 cut(s) 147
AgsI TTSAA 4 cut(s) 40, 266, 331, 745
AjuI GAANNNNNNNTTGG 2 cut(s) 20, 52
AluBI AGCT 5 cut(s) 247, 293, 377, 448, 694
AluI AGCT 5 cut(s) 247, 293, 377, 448, 694
Alw26I GTCTC 2 cut(s) 278, 724
AoxI GGCC 3 cut(s) 30, 484, 624
ApeKI GCWGC 6 cut(s) 247, 287, 290, 445, 494, 614
AsiGI ACCGGT 1 cut(s) 147
Asp700I GAANNNNTTC 1 cut(s) 740
AspS9I GGNCC 3 cut(s) 220, 460, 624
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 224
AvaII GGWCC 2 cut(s) 220, 460
BalI TGGCCA 1 cut(s) 486
BarI GAAGNNNNNNTAC 2 cut(s) 683, 715
BauI CACGAG 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 338
BbvCI CCTCAGC 1 cut(s) 456
BbvI GCAGC 6 cut(s) 234, 274, 302, 457, 481, 626
BccI CCATC 4 cut(s) 337, 471, 508, 626
BceAI ACGGC 1 cut(s) 306
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 2 cut(s) 278, 724
BfmI CTRYAG 1 cut(s) 288
BglI GCCNNNNNGGC 1 cut(s) 623
BisI GCNGC 7 cut(s) 248, 288, 291, 446, 495, 615, 753
BlsI GCNGC 7 cut(s) 249, 289, 292, 447, 496, 616, 754
Bme1390I CCNGG 1 cut(s) 92
Bme18I GGWCC 2 cut(s) 220, 460
BmgT120I GGNCC 3 cut(s) 220, 460, 624
BmiI GGNNCC 1 cut(s) 47
BmrFI CCNGG 1 cut(s) 92
BmrI ACTGGG 1 cut(s) 312
BmsI GCATC 3 cut(s) 236, 337, 442
BmuI ACTGGG 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 338
Bpu10I CCTNAGC 1 cut(s) 456
BpuEI CTTGAG 2 cut(s) 359, 671
BpuMI CCSGG 1 cut(s) 92
BsaI GGTCTC 1 cut(s) 278
BsaJI CCNNGG 3 cut(s) 12, 27, 432
BsaWI WCCGGW 1 cut(s) 147
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 257
Bse118I RCCGGY 1 cut(s) 147
Bse1I ACTGG 1 cut(s) 307
Bse3DI GCAATG 1 cut(s) 370
BseDI CCNNGG 3 cut(s) 12, 27, 432
BseGI GGATG 2 cut(s) 352, 526
BseLI CCNNNNNNNGG 2 cut(s) 147, 257
BseMI GCAATG 1 cut(s) 370
BseMII CTCAG 4 cut(s) 200, 342, 447, 762
BseNI ACTGG 1 cut(s) 307
BseXI GCAGC 6 cut(s) 234, 274, 302, 457, 481, 626
BsgI GTGCAG 1 cut(s) 283
BshFI GGCC 3 cut(s) 32, 486, 626
BshTI ACCGGT 1 cut(s) 147
BsiSI CCGG 2 cut(s) 91, 148
BslI CCNNNNNNNGG 2 cut(s) 147, 257
BsmAI GTCTC 2 cut(s) 278, 724
BsmI GAATGC 1 cut(s) 742
BsnI GGCC 3 cut(s) 32, 486, 626
Bso31I GGTCTC 1 cut(s) 278
Bsp143I GATC 1 cut(s) 189
BspACI CCGC 1 cut(s) 753
BspANI GGCC 3 cut(s) 32, 486, 626
BspCNI CTCAG 4 cut(s) 199, 343, 448, 761
BspLI GGNNCC 1 cut(s) 47
BspMAI CTGCAG 1 cut(s) 292
BspTNI GGTCTC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 370
BsrFI RCCGGY 1 cut(s) 147
BsrI ACTGG 1 cut(s) 307
BssAI RCCGGY 1 cut(s) 147
BssECI CCNNGG 3 cut(s) 12, 27, 432
BssMI GATC 1 cut(s) 189
BssSI CACGAG 1 cut(s) 242
BssT1I CCWWGG 3 cut(s) 12, 27, 432
Bst2BI CACGAG 1 cut(s) 242
Bst4CI ACNGT 2 cut(s) 386, 407
Bst6I CTCTTC 1 cut(s) 585
BstC8I GCNNGC 3 cut(s) 202, 319, 612
BstDEI CTNAG 4 cut(s) 186, 351, 456, 748
BstF5I GGATG 2 cut(s) 352, 526
BstKTI GATC 1 cut(s) 192
BstMAI GTCTC 2 cut(s) 278, 724
BstMBI GATC 1 cut(s) 189
BstMWI GCNNNNNNNGC 3 cut(s) 206, 623, 758
BstNSI RCATGY 1 cut(s) 614
BstSCI CCNGG 1 cut(s) 90
BstSFI CTRYAG 1 cut(s) 288
BstV1I GCAGC 6 cut(s) 234, 274, 302, 457, 481, 626
BstV2I GAAGAC 1 cut(s) 338
BsuRI GGCC 3 cut(s) 32, 486, 626
BtgZI GCGATG 1 cut(s) 449
BtsCI GGATG 2 cut(s) 352, 526
BtsIMutI CAGTG 3 cut(s) 300, 412, 556
Cac8I GCNNGC 3 cut(s) 202, 319, 612
Cfr10I RCCGGY 1 cut(s) 147
Cfr13I GGNCC 3 cut(s) 220, 460, 624
Csp6I GTAC 2 cut(s) 58, 145
CspAI ACCGGT 1 cut(s) 147
CviAII CATG 4 cut(s) 488, 502, 611, 758
CviQI GTAC 2 cut(s) 58, 145
DdeI CTNAG 4 cut(s) 186, 351, 456, 748
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
DraIII CACNNNGTG 1 cut(s) 407
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 1 cut(s) 585
EarI CTCTTC 1 cut(s) 585
Eco130I CCWWGG 3 cut(s) 12, 27, 432
Eco147I AGGCCT 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 278
Eco47I GGWCC 2 cut(s) 220, 460
EcoT14I CCWWGG 3 cut(s) 12, 27, 432
ErhI CCWWGG 3 cut(s) 12, 27, 432
FaeI CATG 4 cut(s) 491, 505, 614, 761
FalI AAGNNNNNCTT 2 cut(s) 20, 52
FatI CATG 4 cut(s) 487, 501, 610, 757
Fnu4HI GCNGC 7 cut(s) 248, 288, 291, 446, 495, 615, 753
FokI GGATG 2 cut(s) 359, 533
Fsp4HI GCNGC 7 cut(s) 248, 288, 291, 446, 495, 615, 753
GluI GCNGC 7 cut(s) 248, 288, 291, 446, 495, 615, 753
HaeIII GGCC 3 cut(s) 32, 486, 626
HapII CCGG 2 cut(s) 91, 148
Hin1II CATG 4 cut(s) 491, 505, 614, 761
HindIII AAGCTT 1 cut(s) 692
HinfI GANTC 1 cut(s) 99
HpaII CCGG 2 cut(s) 91, 148
HphI GGTGA 1 cut(s) 224
Hpy188I TCNGA 4 cut(s) 189, 540, 649, 667
Hpy188III TCNNGA 5 cut(s) 40, 116, 242, 328, 688
Hpy99I CGWCG 1 cut(s) 773
HpyAV CCTTC 2 cut(s) 60, 751
HpyCH4III ACNGT 2 cut(s) 386, 407
HpyCH4V TGCA 5 cut(s) 290, 300, 497, 610, 614
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 623, 758
HpyF3I CTNAG 4 cut(s) 186, 351, 456, 748
Hsp92II CATG 4 cut(s) 491, 505, 614, 761
Kzo9I GATC 1 cut(s) 189
LmnI GCTCC 1 cut(s) 374
LpnPI CCDG 9 cut(s) 88, 104, 161, 195, 214, 288, 408, 540, 607
Lsp1109I GCAGC 6 cut(s) 234, 274, 302, 457, 481, 626
LweI GCATC 3 cut(s) 236, 337, 442
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 4 cut(s) 343, 602, 652, 724
MlsI TGGCCA 1 cut(s) 486
MluCI AATT 1 cut(s) 719
MluNI TGGCCA 1 cut(s) 486
Mox20I TGGCCA 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 740
MscI TGGCCA 1 cut(s) 486
MseI TTAA 2 cut(s) 561, 681
MslI CAYNNNNRTG 2 cut(s) 549, 762
Msp20I TGGCCA 1 cut(s) 486
MspI CCGG 2 cut(s) 91, 148
MspR9I CCNGG 1 cut(s) 92
Mva1269I GAATGC 1 cut(s) 742
MwoI GCNNNNNNNGC 3 cut(s) 206, 623, 758
NciI CCSGG 1 cut(s) 92
NdeII GATC 1 cut(s) 189
NlaIII CATG 4 cut(s) 491, 505, 614, 761
NlaIV GGNNCC 1 cut(s) 47
NspI RCATGY 1 cut(s) 614
PaeI GCATGC 1 cut(s) 614
PceI AGGCCT 1 cut(s) 32
PctI GAATGC 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 740
PfeI GAWTC 1 cut(s) 99
PflMI CCANNNNNTGG 1 cut(s) 257
PinAI ACCGGT 1 cut(s) 147
PkrI GCNGC 7 cut(s) 249, 289, 292, 447, 496, 616, 754
PspN4I GGNNCC 1 cut(s) 47
PspPI GGNCC 3 cut(s) 220, 460, 624
PstI CTGCAG 1 cut(s) 292
RsaI GTAC 2 cut(s) 59, 146
RsaNI GTAC 2 cut(s) 58, 145
RseI CAYNNNNRTG 2 cut(s) 549, 762
SaqAI TTAA 2 cut(s) 561, 681
SatI GCNGC 7 cut(s) 248, 288, 291, 446, 495, 615, 753
Sau3AI GATC 1 cut(s) 189
Sau96I GGNCC 3 cut(s) 220, 460, 624
ScrFI CCNGG 1 cut(s) 92
SfaNI GCATC 3 cut(s) 236, 337, 442
SfcI CTRYAG 1 cut(s) 288
SinI GGWCC 2 cut(s) 220, 460
SmiMI CAYNNNNRTG 2 cut(s) 549, 762
SmlI CTYRAG 2 cut(s) 338, 686
SmoI CTYRAG 2 cut(s) 338, 686
SphI GCATGC 1 cut(s) 614
Sse9I AATT 1 cut(s) 719
SseBI AGGCCT 1 cut(s) 32
SsiI CCGC 1 cut(s) 753
StuI AGGCCT 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 90
StyI CCWWGG 3 cut(s) 12, 27, 432
TaaI ACNGT 2 cut(s) 386, 407
TaqI TCGA 1 cut(s) 588
TasI AATT 1 cut(s) 719
TatI WGTACW 1 cut(s) 57
TauI GCSGC 1 cut(s) 755
TfiI GAWTC 1 cut(s) 99
Tru1I TTAA 2 cut(s) 561, 681
Tru9I TTAA 2 cut(s) 561, 681
TscAI CASTG 3 cut(s) 307, 412, 556
TseI GCWGC 6 cut(s) 247, 287, 290, 445, 494, 614
TspDTI ATGAA 2 cut(s) 537, 653
TspRI CASTG 3 cut(s) 307, 412, 556
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 220, 460
XceI RCATGY 1 cut(s) 614
XmnI GAANNNNTTC 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.