MD15G1148000.v1.1

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001819
Physical Location & Seq
Reverse (-)
10955042 .. 10958059
3018 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1148000.v1.1.491

Sequence Viewer

Length: 900 bp
ATGCCGTTCGTCAAGTCTCAAAAGTCTAGGGCTTACTTCAAGAGGTTTCAAGTCAAGTATAAGAGAAGGAGAGAGGGGAAGACCGACTACCGTGCCAGGATTAGGCTCATTAACCAAGATAAGAACAAGTACAACACACCCAAGTATCGGTTCGTTGTGCGATTTACCAACAAGGATATTGTTGCCCAAATTGTATCTGCCAGCATTGCTGGTGATCTCGTTCTCGCTGCAGCTTATGCTCATGAGCTGCCACACTATGGGCTTGAAGTTGGTCTCACAAACTATGCAGCAGCCTACTGCACTGGCCTTCTCTTGGCTCGTCGTGTGTTGAAGAAGCTTGAAATGGATGACGAATATGAGGGCAATGTTGAGGCTACTGGTGAGGACTACTCTGTGGAGCCAGCTGAGAGCAGAAGGCCATTCCGTGCTCTTCTTGATGTTGGCTTGATCAGGACCACCACAGGAAACCGTGTATTCGGTGCCTTGAAGGGTGCTTTGGATGGGGGATTAGATATCCCGCACAGTGAGAAGAGGTTTGCTGGTTTCTCCAAGGATAGCAAACAGCTTGACGCTGAGGTTCATCGCAAATATATCTATGGAGGCCATGTTGCGGCGTACATGAATACTTTGGGGGAGGATGAGCCAGAGAAATATCAGACTCACTTCAGTGAATATATCAAGAAAGGTATTGAAGCAGATAACATTGAGGAGTTGTACAAGAAAGTTCATGCTGCCATTCGCGCTGATCCTACCGCTAAGAAAACTGAGAAGCAGCCGCCTAAGGAACACAAGAGGTACAATCTCAAGAAGCTTACCTTTGATGAAAGGAAGAAGAAGTTGGTCGAGAGGTTGACTGCCTTCAACGACGCAGCTAACGAGGACGACGATGATGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

300

Amino Acids

34.31

Weight (kDa)

9.17

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L5e PF17144 14 - 175 3.1e-82 Ribosomal large subunit proteins 60S L5, and 50S L18
Ribosomal_L18_c PF14204 235 - 288 6.4e-19 Ribosomal L18 C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 479
AccB7I CCANNNNNTGG 1 cut(s) 257
AccII CGCG 1 cut(s) 741
AciI CCGC 4 cut(s) 518, 611, 753, 776
AclWI GGATC 1 cut(s) 740
AcuI CTGAAG 1 cut(s) 649
AfaI GTAC 4 cut(s) 131, 617, 716, 797
AfiI CCNNNNNNNGG 5 cut(s) 102, 147, 257, 313, 610
AgsI TTSAA 8 cut(s) 40, 50, 266, 331, 341, 487, 692, 862
AjnI CCWGG 1 cut(s) 95
AjuI GAANNNNNNNTTGG 6 cut(s) 134, 166, 479, 511, 821, 853
AleI CACNNNNGTG 1 cut(s) 666
AluBI AGCT 7 cut(s) 233, 247, 337, 404, 565, 811, 872
AluI AGCT 7 cut(s) 233, 247, 337, 404, 565, 811, 872
Alw21I GWGCWC 1 cut(s) 430
Alw26I GTCTC 2 cut(s) 21, 278
AlwI GGATC 1 cut(s) 740
AoxI GGCC 3 cut(s) 304, 416, 601
ApeKI GCWGC 8 cut(s) 227, 230, 247, 287, 290, 731, 772, 869
AspLEI GCGC 1 cut(s) 743
AspS9I GGNCC 1 cut(s) 453
AsuHPI GGTGA 2 cut(s) 224, 392
AvaII GGWCC 1 cut(s) 453
AxyI CCTNAGG 1 cut(s) 780
BaeI ACNNNNGTAYC 2 cut(s) 128, 161
BanI GGYRCC 1 cut(s) 479
BarI GAAGNNNNNNTAC 2 cut(s) 71, 103
BbsI GAAGAC 1 cut(s) 86
Bbv12I GWGCWC 1 cut(s) 430
BbvCI CCTCAGC 1 cut(s) 573
BbvI GCAGC 8 cut(s) 214, 234, 242, 299, 302, 718, 784, 881
BccI CCATC 1 cut(s) 494
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BciT130I CCWGG 1 cut(s) 97
BclI TGATCA 1 cut(s) 447
BcoDI GTCTC 2 cut(s) 21, 278
BfaI CTAG 1 cut(s) 27
BfmI CTRYAG 1 cut(s) 228
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 453
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 2 cut(s) 399, 481
BmrFI CCNGG 1 cut(s) 97
BpiI GAAGAC 1 cut(s) 86
BplI GAGNNNNNCTC 2 cut(s) 374, 406
Bpu10I CCTNAGC 1 cut(s) 573
BpuEI CTTGAG 1 cut(s) 788
BsaI GGTCTC 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 549
BsaXI ACNNNNNCTCC 2 cut(s) 591, 621
Bsc4I CCNNNNNNNGG 5 cut(s) 102, 147, 257, 313, 610
Bse1I ACTGG 2 cut(s) 307, 382
Bse21I CCTNAGG 1 cut(s) 780
Bse3DI GCAATG 2 cut(s) 204, 370
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 549
BseGI GGATG 3 cut(s) 352, 505, 643
BseLI CCNNNNNNNGG 5 cut(s) 102, 147, 257, 313, 610
BseMI GCAATG 2 cut(s) 204, 370
BseMII CTCAG 3 cut(s) 396, 564, 756
BseNI ACTGG 2 cut(s) 307, 382
BseRI GAGGAG 1 cut(s) 722
BseXI GCAGC 8 cut(s) 214, 234, 242, 299, 302, 718, 784, 881
BsgI GTGCAG 1 cut(s) 283
Bsh1236I CGCG 1 cut(s) 741
BshFI GGCC 3 cut(s) 306, 418, 603
BshNI GGYRCC 1 cut(s) 479
BsiHKAI GWGCWC 1 cut(s) 430
BslI CCNNNNNNNGG 5 cut(s) 102, 147, 257, 313, 610
BsmAI GTCTC 2 cut(s) 21, 278
BsnI GGCC 3 cut(s) 306, 418, 603
Bso31I GGTCTC 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 430
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 3 cut(s) 214, 447, 745
BspACI CCGC 4 cut(s) 518, 611, 753, 776
BspANI GGCC 3 cut(s) 306, 418, 603
BspCNI CTCAG 3 cut(s) 397, 565, 757
BspFNI CGCG 1 cut(s) 741
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 399, 481
BspMAI CTGCAG 1 cut(s) 232
BspPI GGATC 1 cut(s) 740
BspQI GCTCTTC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 479
BspTNI GGTCTC 1 cut(s) 278
BsrDI GCAATG 2 cut(s) 204, 370
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 2 cut(s) 307, 382
BssECI CCNNGG 1 cut(s) 549
BssMI GATC 3 cut(s) 214, 447, 745
BssT1I CCWWGG 1 cut(s) 549
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 3 cut(s) 92, 470, 524
Bst6I CTCTTC 2 cut(s) 435, 524
BstAPI GCANNNNNTGC 1 cut(s) 236
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 2 cut(s) 202, 402
BstDEI CTNAG 5 cut(s) 405, 573, 756, 765, 780
BstF5I GGATG 3 cut(s) 352, 505, 643
BstFNI CGCG 1 cut(s) 741
BstHHI GCGC 1 cut(s) 743
BstKTI GATC 3 cut(s) 217, 450, 748
BstMAI GTCTC 2 cut(s) 21, 278
BstMBI GATC 3 cut(s) 214, 447, 745
BstMWI GCNNNNNNNGC 3 cut(s) 206, 236, 740
BstNI CCWGG 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 95
BstSFI CTRYAG 1 cut(s) 228
BstUI CGCG 1 cut(s) 741
BstV1I GCAGC 8 cut(s) 214, 234, 242, 299, 302, 718, 784, 881
BstV2I GAAGAC 1 cut(s) 86
Bsu36I CCTNAGG 1 cut(s) 780
BsuRI GGCC 3 cut(s) 306, 418, 603
BtgZI GCGATG 1 cut(s) 566
BtsCI GGATG 3 cut(s) 352, 505, 643
BtsIMutI CAGTG 3 cut(s) 300, 529, 673
Cac8I GCNNGC 2 cut(s) 202, 402
CciI TCATGA 1 cut(s) 241
CfoI GCGC 1 cut(s) 743
Cfr13I GGNCC 1 cut(s) 453
CseI GACGC 2 cut(s) 578, 875
Csp6I GTAC 4 cut(s) 130, 616, 715, 796
CviAII CATG 4 cut(s) 242, 605, 619, 728
CviQI GTAC 4 cut(s) 130, 616, 715, 796
DdeI CTNAG 5 cut(s) 405, 573, 756, 765, 780
DpnI GATC 3 cut(s) 216, 449, 747
DpnII GATC 3 cut(s) 214, 447, 745
Eam1104I CTCTTC 2 cut(s) 435, 524
EarI CTCTTC 2 cut(s) 435, 524
Eco130I CCWWGG 1 cut(s) 549
Eco31I GGTCTC 1 cut(s) 278
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 453
Eco57I CTGAAG 1 cut(s) 649
Eco81I CCTNAGG 1 cut(s) 780
EcoRII CCWGG 1 cut(s) 95
EcoRV GATATC 1 cut(s) 514
EcoT14I CCWWGG 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 549
FaeI CATG 4 cut(s) 245, 608, 622, 731
FalI AAGNNNNNCTT 2 cut(s) 800, 832
FatI CATG 4 cut(s) 241, 604, 618, 727
FauI CCCGC 1 cut(s) 525
FbaI TGATCA 1 cut(s) 447
FokI GGATG 3 cut(s) 359, 512, 650
FspBI CTAG 1 cut(s) 27
GlaI GCGC 1 cut(s) 742
HaeIII GGCC 3 cut(s) 306, 418, 603
HgaI GACGC 2 cut(s) 578, 875
HhaI GCGC 1 cut(s) 743
Hin1II CATG 4 cut(s) 245, 608, 622, 731
Hin6I GCGC 1 cut(s) 741
HinP1I GCGC 1 cut(s) 741
HincII GTYRAC 1 cut(s) 852
HindII GTYRAC 1 cut(s) 852
HindIII AAGCTT 2 cut(s) 335, 809
HinfI GANTC 1 cut(s) 658
HphI GGTGA 2 cut(s) 224, 392
Hpy166II GTNNAC 1 cut(s) 852
Hpy188I TCNGA 1 cut(s) 657
Hpy188III TCNNGA 7 cut(s) 40, 242, 434, 451, 679, 805, 844
Hpy8I GTNNAC 1 cut(s) 852
Hpy99I CGWCG 3 cut(s) 324, 869, 887
HpyAV CCTTC 5 cut(s) 60, 317, 408, 481, 868
HpyCH4III ACNGT 3 cut(s) 92, 470, 524
HpyCH4V TGCA 3 cut(s) 230, 287, 300
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 236, 740
HpyF3I CTNAG 5 cut(s) 405, 573, 756, 765, 780
Hsp92II CATG 4 cut(s) 245, 608, 622, 731
HspAI GCGC 1 cut(s) 741
Ksp22I TGATCA 1 cut(s) 447
Kzo9I GATC 3 cut(s) 214, 447, 745
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 1 cut(s) 397
Lsp1109I GCAGC 8 cut(s) 214, 234, 242, 299, 302, 718, 784, 881
MaeI CTAG 1 cut(s) 27
MalI GATC 3 cut(s) 216, 449, 747
MboI GATC 3 cut(s) 214, 447, 745
MboII GAAGA 6 cut(s) 91, 343, 422, 541, 841, 844
MhlI GDGCHC 1 cut(s) 430
MluCI AATT 1 cut(s) 189
MlyI GAGTC 1 cut(s) 652
MseI TTAA 1 cut(s) 111
MslI CAYNNNNRTG 1 cut(s) 666
MspA1I CMGCKG 1 cut(s) 404
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MvnI CGCG 1 cut(s) 741
MwoI GCNNNNNNNGC 3 cut(s) 206, 236, 740
NdeII GATC 3 cut(s) 214, 447, 745
NlaIII CATG 4 cut(s) 245, 608, 622, 731
NlaIV GGNNCC 2 cut(s) 399, 481
OliI CACNNNNGTG 1 cut(s) 666
PagI TCATGA 1 cut(s) 241
PciSI GCTCTTC 1 cut(s) 435
PcsI WCGNNNNNNNCGW 3 cut(s) 873, 882, 891
PflMI CCANNNNNTGG 1 cut(s) 257
PleI GAGTC 1 cut(s) 652
PpsI GAGTC 1 cut(s) 652
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 2 cut(s) 399, 481
PspPI GGNCC 1 cut(s) 453
PstI CTGCAG 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 4 cut(s) 131, 617, 716, 797
RsaNI GTAC 4 cut(s) 130, 616, 715, 796
RseI CAYNNNNRTG 1 cut(s) 666
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 1 cut(s) 111
Sau3AI GATC 3 cut(s) 214, 447, 745
Sau96I GGNCC 1 cut(s) 453
SchI GAGTC 1 cut(s) 652
ScrFI CCNGG 1 cut(s) 97
SduI GDGCHC 1 cut(s) 430
SfcI CTRYAG 1 cut(s) 228
SinI GGWCC 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 803
SmoI CTYRAG 1 cut(s) 803
Sse9I AATT 1 cut(s) 189
SsiI CCGC 4 cut(s) 518, 611, 753, 776
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 1 cut(s) 95
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 3 cut(s) 92, 470, 524
TaqI TCGA 1 cut(s) 843
TaqII GACCGA 1 cut(s) 98
TasI AATT 1 cut(s) 189
TatI WGTACW 2 cut(s) 129, 714
TauI GCSGC 2 cut(s) 614, 778
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 3 cut(s) 307, 529, 673
TseI GCWGC 8 cut(s) 227, 230, 247, 287, 290, 731, 772, 869
TspDTI ATGAA 4 cut(s) 569, 635, 716, 837
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 3 cut(s) 307, 529, 673
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 453
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.