MD15G1147900.v1.1

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001819
Physical Location & Seq
Reverse (-)
10951444 .. 10954344
2901 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1147900.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGCTTTCGCCAAAGCTCAGAAATCAAGGGCCTACTTCAAGAGGTTCCAAGTCAAGTACAAGAGAAGGCGAGAGGGAAAGACTGACTACCGGGCCAGGATTAGGCTTATTAACCAAGACAAGAACAAGTACAACACACCCAAGTATCGGTTTGTTGTGAGATTTACCAACAAAGATATTGTTGCACAAATTACATCTGCCAGTATTGCTGGTGATTTGGTTCTTGCTGCTGCCTATGCCCATGAGCTCCCACGCTATGGGCTCGAAGTTGGTCTTACAAACTATGCTGCAGCCTATTGCACTGGCCTTCTTTTGGCTCGTCGTGTGTTGAAGAAGCTTGAAATGGATGACGAATATGAGGGAAATGTTGAGGCTACTGGTGAGGACTATTCTGTAGAGCCAGCTGAGAGCAGAAGGCCATTCCGTGCTCTTCTTGATGTAGGTCTTATCAGGACCACCACGGGAAATCGCGTATTCGGTGCGTTGAAGGGTGCTTTGGATGGAGGGTTGGATATCCCTCACAGTGAAAAGAGGTTTGCCGGCTTTTCCAAGGATAGCAAGCAACTTGACCCTGAGGTTCATCGGAAGTACATCTATGGTGGCCATGTTGCTGCCTACATGACGACCTTGGCGGAGGATGAGCCTGAGAAGTATCAAAGTCACTTCAGTGAATATATTAAGAAAGGTATTGAAGCGGATAACATCGAGGAGTTGTACAAGAAAGTTCATGCAGCCATCCGTGCTGATCCTACAATTAAGAAGACTGAAAAGCCTGCTCCTCAAGAACACAAGAGATACAACCTCAAAAAGCTCACCTTTGATGAGAGGAAGATGAAGTTGGTTGAGAGATTGAAGGCCTTCAATGACGCTGGTAACGATGACAACAGTGATGATGATGATGAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

303

Amino Acids

34.63

Weight (kDa)

9.17

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L5e PF17144 14 - 175 3.2e-82 Ribosomal large subunit proteins 60S L5, and 50S L18
Ribosomal_L18_c PF14204 235 - 288 2.1e-18 Ribosomal L18 C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 257
AccII CGCG 1 cut(s) 471
AciI CCGC 2 cut(s) 632, 695
AclWI GGATC 1 cut(s) 740
AcoI YGGCCR 1 cut(s) 601
AcuI CTGAAG 1 cut(s) 649
AfaI GTAC 5 cut(s) 59, 131, 590, 716, 905
AfiI CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
AgsI TTSAA 7 cut(s) 40, 331, 341, 487, 692, 853, 862
AjnI CCWGG 1 cut(s) 95
AjuI GAANNNNNNNTTGG 4 cut(s) 479, 511, 821, 853
AleI CACNNNNGTG 1 cut(s) 666
AluBI AGCT 5 cut(s) 17, 247, 337, 404, 811
AluI AGCT 5 cut(s) 17, 247, 337, 404, 811
Alw21I GWGCWC 2 cut(s) 249, 430
AlwI GGATC 1 cut(s) 740
AoxI GGCC 6 cut(s) 30, 93, 304, 416, 601, 855
ApeKI GCWGC 6 cut(s) 227, 230, 287, 290, 611, 731
Asp700I GAANNNNTTC 1 cut(s) 857
AspS9I GGNCC 3 cut(s) 30, 93, 453
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 3 cut(s) 224, 392, 805
AvaII GGWCC 1 cut(s) 453
AxyI CCTNAGG 1 cut(s) 573
BaeI ACNNNNGTAYC 2 cut(s) 128, 161
BalI TGGCCA 1 cut(s) 603
BanII GRGCYC 2 cut(s) 249, 264
BbsI GAAGAC 1 cut(s) 767
Bbv12I GWGCWC 2 cut(s) 249, 430
BbvI GCAGC 6 cut(s) 214, 217, 274, 302, 598, 743
BccI CCATC 2 cut(s) 494, 743
BciT130I CCWGG 1 cut(s) 97
BcnI CCSGG 1 cut(s) 92
BfmI CTRYAG 2 cut(s) 288, 393
BisI GCNGC 6 cut(s) 228, 231, 288, 291, 612, 732
BlsI GCNGC 6 cut(s) 229, 232, 289, 292, 613, 733
BmcAI AGTACT 1 cut(s) 905
Bme1390I CCNGG 2 cut(s) 92, 97
Bme18I GGWCC 1 cut(s) 453
BmgT120I GGNCC 3 cut(s) 30, 93, 453
BmiI GGNNCC 1 cut(s) 47
BmrFI CCNGG 2 cut(s) 92, 97
BpiI GAAGAC 1 cut(s) 767
BpuEI CTTGAG 1 cut(s) 765
BpuMI CCSGG 1 cut(s) 92
BsaJI CCNNGG 3 cut(s) 459, 549, 627
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
Bse118I RCCGGY 1 cut(s) 539
Bse1I ACTGG 3 cut(s) 201, 307, 382
Bse21I CCTNAGG 1 cut(s) 573
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 3 cut(s) 459, 549, 627
BseGI GGATG 4 cut(s) 352, 505, 643, 735
BseLI CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
BseMII CTCAG 4 cut(s) 32, 396, 564, 636
BseNI ACTGG 3 cut(s) 201, 307, 382
BseRI GAGGAG 2 cut(s) 722, 768
BseXI GCAGC 6 cut(s) 214, 217, 274, 302, 598, 743
Bsh1236I CGCG 1 cut(s) 471
BshFI GGCC 6 cut(s) 32, 95, 306, 418, 603, 857
BsiHKAI GWGCWC 2 cut(s) 249, 430
BsiSI CCGG 2 cut(s) 91, 540
BslI CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
BsnI GGCC 6 cut(s) 32, 95, 306, 418, 603, 857
Bsp1286I GDGCHC 3 cut(s) 249, 264, 430
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 1 cut(s) 745
BspACI CCGC 2 cut(s) 632, 695
BspANI GGCC 6 cut(s) 32, 95, 306, 418, 603, 857
BspCNI CTCAG 4 cut(s) 31, 397, 565, 637
BspFNI CGCG 1 cut(s) 471
BspLI GGNNCC 1 cut(s) 47
BspMAI CTGCAG 1 cut(s) 292
BspPI GGATC 1 cut(s) 740
BspQI GCTCTTC 1 cut(s) 435
BsrFI RCCGGY 1 cut(s) 539
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 3 cut(s) 201, 307, 382
BssAI RCCGGY 1 cut(s) 539
BssECI CCNNGG 3 cut(s) 459, 549, 627
BssMI GATC 1 cut(s) 745
BssT1I CCWWGG 2 cut(s) 549, 627
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 2 cut(s) 524, 887
Bst6I CTCTTC 1 cut(s) 435
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 4 cut(s) 402, 541, 560, 774
BstDEI CTNAG 4 cut(s) 18, 405, 573, 645
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 4 cut(s) 352, 505, 643, 735
BstFNI CGCG 1 cut(s) 471
BstKTI GATC 1 cut(s) 748
BstMBI GATC 1 cut(s) 745
BstMWI GCNNNNNNNGC 3 cut(s) 206, 236, 740
BstNI CCWGG 1 cut(s) 97
BstSCI CCNGG 2 cut(s) 90, 95
BstSFI CTRYAG 2 cut(s) 288, 393
BstUI CGCG 1 cut(s) 471
BstV1I GCAGC 6 cut(s) 214, 217, 274, 302, 598, 743
BstV2I GAAGAC 1 cut(s) 767
Bsu36I CCTNAGG 1 cut(s) 573
BsuRI GGCC 6 cut(s) 32, 95, 306, 418, 603, 857
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 4 cut(s) 352, 505, 643, 735
BtsIMutI CAGTG 4 cut(s) 300, 529, 673, 892
Cac8I GCNNGC 4 cut(s) 402, 541, 560, 774
Cfr10I RCCGGY 1 cut(s) 539
Cfr13I GGNCC 3 cut(s) 30, 93, 453
CseI GACGC 1 cut(s) 875
Csp6I GTAC 5 cut(s) 58, 130, 589, 715, 904
CviAII CATG 4 cut(s) 242, 605, 619, 728
CviQI GTAC 5 cut(s) 58, 130, 589, 715, 904
DdeI CTNAG 4 cut(s) 18, 405, 573, 645
DpnI GATC 1 cut(s) 747
DpnII GATC 1 cut(s) 745
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
EciI GGCGGA 1 cut(s) 647
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 2 cut(s) 549, 627
Eco147I AGGCCT 1 cut(s) 857
Eco24I GRGCYC 2 cut(s) 249, 264
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 453
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 649
Eco81I CCTNAGG 1 cut(s) 573
EcoICRI GAGCTC 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 30
EcoRII CCWGG 1 cut(s) 95
EcoRV GATATC 1 cut(s) 514
EcoT14I CCWWGG 2 cut(s) 549, 627
EcoT38I GRGCYC 2 cut(s) 249, 264
ErhI CCWWGG 2 cut(s) 549, 627
FaeI CATG 4 cut(s) 245, 608, 622, 731
FalI AAGNNNNNCTT 4 cut(s) 258, 290, 800, 832
FatI CATG 4 cut(s) 241, 604, 618, 727
Fnu4HI GCNGC 6 cut(s) 228, 231, 288, 291, 612, 732
FokI GGATG 4 cut(s) 359, 512, 650, 722
FriOI GRGCYC 2 cut(s) 249, 264
Fsp4HI GCNGC 6 cut(s) 228, 231, 288, 291, 612, 732
GluI GCNGC 6 cut(s) 228, 231, 288, 291, 612, 732
HaeIII GGCC 6 cut(s) 32, 95, 306, 418, 603, 857
HapII CCGG 2 cut(s) 91, 540
HgaI GACGC 1 cut(s) 875
Hin1II CATG 4 cut(s) 245, 608, 622, 731
HindIII AAGCTT 1 cut(s) 335
HpaII CCGG 2 cut(s) 91, 540
HphI GGTGA 3 cut(s) 224, 392, 805
Hpy188I TCNGA 2 cut(s) 21, 585
Hpy188III TCNNGA 4 cut(s) 40, 434, 451, 782
Hpy99I CGWCG 1 cut(s) 324
HpyAV CCTTC 6 cut(s) 60, 317, 408, 481, 847, 868
HpyCH4III ACNGT 2 cut(s) 524, 887
HpyCH4V TGCA 4 cut(s) 185, 290, 300, 731
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 236, 740
HpyF3I CTNAG 4 cut(s) 18, 405, 573, 645
Hsp92II CATG 4 cut(s) 245, 608, 622, 731
KroI GCCGGC 1 cut(s) 539
KroNI GCCGGC 1 cut(s) 541
Kzo9I GATC 1 cut(s) 745
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 2 cut(s) 252, 781
Lsp1109I GCAGC 6 cut(s) 214, 217, 274, 302, 598, 743
MaeIII GTNAC 2 cut(s) 659, 872
MalI GATC 1 cut(s) 747
MboI GATC 1 cut(s) 745
MboII GAAGA 4 cut(s) 343, 422, 772, 841
MhlI GDGCHC 3 cut(s) 249, 264, 430
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 2 cut(s) 189, 753
MluNI TGGCCA 1 cut(s) 603
MmeI TCCRAC 1 cut(s) 489
Mox20I TGGCCA 1 cut(s) 603
MroNI GCCGGC 1 cut(s) 539
MroXI GAANNNNTTC 1 cut(s) 857
MscI TGGCCA 1 cut(s) 603
MseI TTAA 3 cut(s) 111, 678, 756
MslI CAYNNNNRTG 1 cut(s) 666
Msp20I TGGCCA 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 2 cut(s) 91, 540
MspR9I CCNGG 2 cut(s) 92, 97
MvaI CCWGG 1 cut(s) 97
MvnI CGCG 1 cut(s) 471
MwoI GCNNNNNNNGC 3 cut(s) 206, 236, 740
NaeI GCCGGC 1 cut(s) 541
NciI CCSGG 1 cut(s) 92
NdeII GATC 1 cut(s) 745
NgoMIV GCCGGC 1 cut(s) 539
NlaIII CATG 4 cut(s) 245, 608, 622, 731
NlaIV GGNNCC 1 cut(s) 47
NmuCI GTSAC 1 cut(s) 659
OliI CACNNNNGTG 1 cut(s) 666
PceI AGGCCT 1 cut(s) 857
PciSI GCTCTTC 1 cut(s) 435
PcsI WCGNNNNNNNCGW 1 cut(s) 873
PdiI GCCGGC 1 cut(s) 541
PdmI GAANNNNTTC 1 cut(s) 857
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 6 cut(s) 229, 232, 289, 292, 613, 733
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 47
PspPI GGNCC 3 cut(s) 30, 93, 453
PstI CTGCAG 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 5 cut(s) 59, 131, 590, 716, 905
RsaNI GTAC 5 cut(s) 58, 130, 589, 715, 904
RseI CAYNNNNRTG 1 cut(s) 666
SacI GAGCTC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 3 cut(s) 111, 678, 756
SatI GCNGC 6 cut(s) 228, 231, 288, 291, 612, 732
Sau3AI GATC 1 cut(s) 745
Sau96I GGNCC 3 cut(s) 30, 93, 453
ScaI AGTACT 1 cut(s) 905
ScrFI CCNGG 2 cut(s) 92, 97
SduI GDGCHC 3 cut(s) 249, 264, 430
SfcI CTRYAG 2 cut(s) 288, 393
SinI GGWCC 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 780
SmoI CTYRAG 1 cut(s) 780
Sse9I AATT 2 cut(s) 189, 753
SseBI AGGCCT 1 cut(s) 857
SsiI CCGC 2 cut(s) 632, 695
SstI GAGCTC 1 cut(s) 249
StuI AGGCCT 1 cut(s) 857
StyD4I CCNGG 2 cut(s) 90, 95
StyI CCWWGG 2 cut(s) 549, 627
TaaI ACNGT 2 cut(s) 524, 887
TaqI TCGA 2 cut(s) 264, 705
TasI AATT 2 cut(s) 189, 753
TatI WGTACW 5 cut(s) 57, 129, 588, 714, 903
Tru1I TTAA 3 cut(s) 111, 678, 756
Tru9I TTAA 3 cut(s) 111, 678, 756
TscAI CASTG 4 cut(s) 307, 529, 673, 892
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 6 cut(s) 227, 230, 287, 290, 611, 731
Tsp45I GTSAC 1 cut(s) 659
TspDTI ATGAA 3 cut(s) 569, 716, 848
TspGWI ACGGA 2 cut(s) 413, 728
TspRI CASTG 4 cut(s) 307, 529, 673, 892
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 857
ZrmI AGTACT 1 cut(s) 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.