Rh2BG006900

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001819
Physical Location & Seq
Reverse (-)
497053 .. 499991
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG006900.1

Sequence Viewer

Length: 900 bp
ATGGCTTTTGCCAAGGCTCACAAGTCCAAGGCCTACTTCAAGAGGTTCCAAGTCAAGTACAAGAGAAGGCGAGAGGGTAAGACTGACTACCGGGCAAGAATCAGGCTCATCAATCAAGACAAGAACAAATACAACACGCCCAAGTACCGGTTTGTTGTGAGATTTACAAACAAGGATATTGTTGCTCAGATCGTATCTGCCAGCATTGCTGGCGATTTGGTCCTTGCATCAGCCTATGCTCATGAGCTGCCACACTTTGGGCTTGAAGTTGGTCCCACAAACTATGCTGCAGCTTACTGCACTGGGCTTCTCTTGGCTCGCCGTGTCCTGAAGACACTTGAGATGGATGCTGAGTATGAGGGCAATGTTGAGGCTACTGGTGAGGATTTCTCTGTTGAGCCAACTGAGAGCAGAAGGCCATTCCGTGCTCTTCTTGATGTTGGCTTAATTAAGACAACTACTGGAAATCGTGTGTTTGGTGCCCTGAAGGGAGCTTTGGACGGTGGTTTGGATATTCCACACAGTGATAAGAGGTTTGCTGGTTTCTCCAAGGACAACAAGCAGCTTGATGCTGAGGTCCATCGCAAGTATATCTATGGTGGCCATGTTGCTGCATACATGAGGACTTTGATGGAGGATGAACCAGAAAAGTATCAGAGCCATTTCAGTGAATACATTAAGAAAGGAATAGGAGCAGATAACATTGAAGAGATTTACAAGAAAGTGCATGCAGCCATCAGGGCCGACCCTACAATGAAGAAATCTGAAAAGCCTCAACGCTCGGAACACAAGAGGTTTAATCTCAAGAAGCTTACCTACGAGGAAAGGAAGAACAAATTGATTGAGAGACTGAATGCCTTCAACTCAGCCGCCCATGCTGATGACTATGACGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

299

Amino Acids

34.24

Weight (kDa)

9.43

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L5e PF17144 14 - 175 5.6e-79 Ribosomal large subunit proteins 60S L5, and 50S L18
Ribosomal_L18_c PF14204 235 - 288 2.9e-19 Ribosomal L18 C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 479
AccB7I CCANNNNNTGG 1 cut(s) 257
AciI CCGC 1 cut(s) 870
AcoI YGGCCR 1 cut(s) 601
AcuI CTGAAG 2 cut(s) 350, 506
AdeI CACNNNGTG 1 cut(s) 524
AfaI GTAC 2 cut(s) 59, 146
AfiI CCNNNNNNNGG 2 cut(s) 147, 257
AgeI ACCGGT 1 cut(s) 147
AgsI TTSAA 4 cut(s) 40, 266, 707, 862
AjuI GAANNNNNNNTTGG 4 cut(s) 20, 52, 479, 511
AluBI AGCT 5 cut(s) 247, 293, 494, 565, 811
AluI AGCT 5 cut(s) 247, 293, 494, 565, 811
Alw21I GWGCWC 1 cut(s) 430
Alw26I GTCTC 1 cut(s) 841
AoxI GGCC 4 cut(s) 30, 416, 601, 741
ApeKI GCWGC 6 cut(s) 247, 287, 290, 562, 611, 731
AsiGI ACCGGT 1 cut(s) 147
Asp700I GAANNNNTTC 1 cut(s) 857
AspS9I GGNCC 4 cut(s) 220, 272, 577, 741
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 392
AvaII GGWCC 3 cut(s) 220, 272, 577
BaeGI GKGCMC 1 cut(s) 484
BalI TGGCCA 1 cut(s) 603
BanI GGYRCC 1 cut(s) 479
BarI GAAGNNNNNNTAC 2 cut(s) 800, 832
BbsI GAAGAC 1 cut(s) 338
Bbv12I GWGCWC 1 cut(s) 430
BbvCI CCTCAGC 1 cut(s) 573
BbvI GCAGC 6 cut(s) 234, 274, 302, 574, 598, 743
BccI CCATC 4 cut(s) 337, 588, 625, 743
BceAI ACGGC 1 cut(s) 306
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 841
BfmI CTRYAG 1 cut(s) 288
BglI GCCNNNNNGGC 1 cut(s) 740
BisI GCNGC 7 cut(s) 248, 288, 291, 563, 612, 732, 870
BlsI GCNGC 7 cut(s) 249, 289, 292, 564, 613, 733, 871
Bme1390I CCNGG 1 cut(s) 92
Bme18I GGWCC 3 cut(s) 220, 272, 577
BmgT120I GGNCC 4 cut(s) 220, 272, 577, 741
BmiI GGNNCC 3 cut(s) 47, 274, 481
BmrFI CCNGG 1 cut(s) 92
BmrI ACTGGG 1 cut(s) 312
BmsI GCATC 3 cut(s) 236, 337, 559
BmuI ACTGGG 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 338
BplI GAGNNNNNCTC 2 cut(s) 374, 406
Bpu10I CCTNAGC 1 cut(s) 573
BpuEI CTTGAG 2 cut(s) 359, 788
BpuMI CCSGG 1 cut(s) 92
BsaJI CCNNGG 3 cut(s) 12, 27, 549
BsaWI WCCGGW 1 cut(s) 147
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 257
Bse118I RCCGGY 1 cut(s) 147
Bse1I ACTGG 3 cut(s) 307, 382, 466
Bse3DI GCAATG 2 cut(s) 204, 370
BseDI CCNNGG 3 cut(s) 12, 27, 549
BseGI GGATG 2 cut(s) 352, 643
BseLI CCNNNNNNNGG 2 cut(s) 147, 257
BseMI GCAATG 2 cut(s) 204, 370
BseMII CTCAG 5 cut(s) 200, 342, 396, 564, 879
BseNI ACTGG 3 cut(s) 307, 382, 466
BseSI GKGCMC 1 cut(s) 484
BseXI GCAGC 6 cut(s) 234, 274, 302, 574, 598, 743
BsgI GTGCAG 1 cut(s) 283
BshFI GGCC 4 cut(s) 32, 418, 603, 743
BshNI GGYRCC 1 cut(s) 479
BshTI ACCGGT 1 cut(s) 147
BsiHKAI GWGCWC 1 cut(s) 430
BsiSI CCGG 2 cut(s) 91, 148
BslFI GGGAC 1 cut(s) 258
BslI CCNNNNNNNGG 2 cut(s) 147, 257
BsmAI GTCTC 1 cut(s) 841
BsmFI GGGAC 1 cut(s) 258
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 4 cut(s) 32, 418, 603, 743
Bsp1286I GDGCHC 2 cut(s) 430, 484
Bsp143I GATC 1 cut(s) 189
BspACI CCGC 1 cut(s) 870
BspANI GGCC 4 cut(s) 32, 418, 603, 743
BspCNI CTCAG 5 cut(s) 199, 343, 397, 565, 878
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 3 cut(s) 47, 274, 481
BspMAI CTGCAG 1 cut(s) 292
BspQI GCTCTTC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 479
BsrDI GCAATG 2 cut(s) 204, 370
BsrFI RCCGGY 1 cut(s) 147
BsrI ACTGG 3 cut(s) 307, 382, 466
BssAI RCCGGY 1 cut(s) 147
BssECI CCNNGG 3 cut(s) 12, 27, 549
BssMI GATC 1 cut(s) 189
BssT1I CCWWGG 3 cut(s) 12, 27, 549
Bst4CI ACNGT 2 cut(s) 503, 524
Bst6I CTCTTC 2 cut(s) 435, 702
BstC8I GCNNGC 4 cut(s) 202, 211, 319, 729
BstDEI CTNAG 5 cut(s) 186, 351, 405, 573, 865
BstF5I GGATG 2 cut(s) 352, 643
BstKTI GATC 1 cut(s) 192
BstMAI GTCTC 1 cut(s) 841
BstMBI GATC 1 cut(s) 189
BstMWI GCNNNNNNNGC 4 cut(s) 206, 210, 740, 875
BstNSI RCATGY 1 cut(s) 731
BstSCI CCNGG 1 cut(s) 90
BstSFI CTRYAG 1 cut(s) 288
BstSLI GKGCMC 1 cut(s) 484
BstV1I GCAGC 6 cut(s) 234, 274, 302, 574, 598, 743
BstV2I GAAGAC 1 cut(s) 338
BsuRI GGCC 4 cut(s) 32, 418, 603, 743
BtgZI GCGATG 1 cut(s) 566
BtsCI GGATG 2 cut(s) 352, 643
BtsIMutI CAGTG 3 cut(s) 300, 529, 673
Cac8I GCNNGC 4 cut(s) 202, 211, 319, 729
CciI TCATGA 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 147
Cfr13I GGNCC 4 cut(s) 220, 272, 577, 741
Csp6I GTAC 2 cut(s) 58, 145
CspAI ACCGGT 1 cut(s) 147
CviAII CATG 5 cut(s) 242, 605, 619, 728, 875
CviQI GTAC 2 cut(s) 58, 145
DdeI CTNAG 5 cut(s) 186, 351, 405, 573, 865
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
DraIII CACNNNGTG 1 cut(s) 524
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 2 cut(s) 435, 702
EarI CTCTTC 2 cut(s) 435, 702
Eco130I CCWWGG 3 cut(s) 12, 27, 549
Eco147I AGGCCT 1 cut(s) 32
Eco47I GGWCC 3 cut(s) 220, 272, 577
Eco57I CTGAAG 2 cut(s) 350, 506
EcoT14I CCWWGG 3 cut(s) 12, 27, 549
ErhI CCWWGG 3 cut(s) 12, 27, 549
FaeI CATG 5 cut(s) 245, 608, 622, 731, 878
FalI AAGNNNNNCTT 2 cut(s) 20, 52
FaqI GGGAC 1 cut(s) 258
FatI CATG 5 cut(s) 241, 604, 618, 727, 874
Fnu4HI GCNGC 7 cut(s) 248, 288, 291, 563, 612, 732, 870
FokI GGATG 2 cut(s) 359, 650
Fsp4HI GCNGC 7 cut(s) 248, 288, 291, 563, 612, 732, 870
GluI GCNGC 7 cut(s) 248, 288, 291, 563, 612, 732, 870
HaeIII GGCC 4 cut(s) 32, 418, 603, 743
HapII CCGG 2 cut(s) 91, 148
Hin1II CATG 5 cut(s) 245, 608, 622, 731, 878
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 1 cut(s) 99
HpaII CCGG 2 cut(s) 91, 148
HphI GGTGA 1 cut(s) 392
Hpy188I TCNGA 4 cut(s) 189, 657, 766, 784
Hpy188III TCNNGA 6 cut(s) 40, 116, 242, 328, 434, 805
HpyAV CCTTC 4 cut(s) 60, 408, 481, 868
HpyCH4III ACNGT 2 cut(s) 503, 524
HpyCH4V TGCA 6 cut(s) 227, 290, 300, 614, 727, 731
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 210, 740, 875
HpyF3I CTNAG 5 cut(s) 186, 351, 405, 573, 865
Hsp92II CATG 5 cut(s) 245, 608, 622, 731, 878
Kzo9I GATC 1 cut(s) 189
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 2 cut(s) 491, 692
Lsp1109I GCAGC 6 cut(s) 234, 274, 302, 574, 598, 743
LweI GCATC 3 cut(s) 236, 337, 559
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 5 cut(s) 343, 422, 719, 769, 841
MhlI GDGCHC 2 cut(s) 430, 484
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 2 cut(s) 447, 836
MluNI TGGCCA 1 cut(s) 603
Mox20I TGGCCA 1 cut(s) 603
MroXI GAANNNNTTC 1 cut(s) 857
MscI TGGCCA 1 cut(s) 603
MseI TTAA 4 cut(s) 446, 450, 678, 798
MslI CAYNNNNRTG 2 cut(s) 666, 879
Msp20I TGGCCA 1 cut(s) 603
MspI CCGG 2 cut(s) 91, 148
MspR9I CCNGG 1 cut(s) 92
Mva1269I GAATGC 1 cut(s) 859
MwoI GCNNNNNNNGC 4 cut(s) 206, 210, 740, 875
NciI CCSGG 1 cut(s) 92
NdeII GATC 1 cut(s) 189
NlaIII CATG 5 cut(s) 245, 608, 622, 731, 878
NlaIV GGNNCC 3 cut(s) 47, 274, 481
NspI RCATGY 1 cut(s) 731
PacI TTAATTAA 1 cut(s) 450
PaeI GCATGC 1 cut(s) 731
PagI TCATGA 1 cut(s) 241
PceI AGGCCT 1 cut(s) 32
PciSI GCTCTTC 1 cut(s) 435
PctI GAATGC 1 cut(s) 859
PdmI GAANNNNTTC 1 cut(s) 857
PfeI GAWTC 1 cut(s) 99
PflMI CCANNNNNTGG 1 cut(s) 257
PinAI ACCGGT 1 cut(s) 147
PkrI GCNGC 7 cut(s) 249, 289, 292, 564, 613, 733, 871
PspN4I GGNNCC 3 cut(s) 47, 274, 481
PspPI GGNCC 4 cut(s) 220, 272, 577, 741
PstI CTGCAG 1 cut(s) 292
RsaI GTAC 2 cut(s) 59, 146
RsaNI GTAC 2 cut(s) 58, 145
RseI CAYNNNNRTG 2 cut(s) 666, 879
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 4 cut(s) 446, 450, 678, 798
SatI GCNGC 7 cut(s) 248, 288, 291, 563, 612, 732, 870
Sau3AI GATC 1 cut(s) 189
Sau96I GGNCC 4 cut(s) 220, 272, 577, 741
ScrFI CCNGG 1 cut(s) 92
SduI GDGCHC 2 cut(s) 430, 484
SfaNI GCATC 3 cut(s) 236, 337, 559
SfcI CTRYAG 1 cut(s) 288
SinI GGWCC 3 cut(s) 220, 272, 577
SmiMI CAYNNNNRTG 2 cut(s) 666, 879
SmlI CTYRAG 2 cut(s) 338, 803
SmoI CTYRAG 2 cut(s) 338, 803
SphI GCATGC 1 cut(s) 731
Sse9I AATT 2 cut(s) 447, 836
SseBI AGGCCT 1 cut(s) 32
SsiI CCGC 1 cut(s) 870
StuI AGGCCT 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 90
StyI CCWWGG 3 cut(s) 12, 27, 549
TaaI ACNGT 2 cut(s) 503, 524
TasI AATT 2 cut(s) 447, 836
TatI WGTACW 1 cut(s) 57
TauI GCSGC 1 cut(s) 872
TfiI GAWTC 1 cut(s) 99
Tru1I TTAA 4 cut(s) 446, 450, 678, 798
Tru9I TTAA 4 cut(s) 446, 450, 678, 798
TscAI CASTG 3 cut(s) 307, 529, 673
TseI GCWGC 6 cut(s) 247, 287, 290, 562, 611, 731
TspDTI ATGAA 2 cut(s) 654, 770
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 3 cut(s) 307, 529, 673
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 3 cut(s) 220, 272, 577
XceI RCATGY 1 cut(s) 731
XmnI GAANNNNTTC 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.