Rw4G003540

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001819
Physical Location & Seq
Reverse (-)
7071633 .. 7072801
1169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G003540.1

Sequence Viewer

Length: 945 bp
ATGGATCCAAAACCCCTACCCCCTCCTCCTCCTCCTCCTCCAATGGCTTTCATCAAAGCTTCAAAACCCCGCGCCTACTTCAAGCGCTTCCAGGTCAAGTACAAGCGCCGCCGCGAAGGCAAGACCGACTACCGCGCCCGCATCCGCCTCATCAACCAGGACAAGAACAAGTACAACACCCCCAAGTACCGCTTCGTCGTCCGCTTCACCAACAAGGACATCGTCGCCCAGATCATCTCCGCCACCATCTCCGGCGACCTCGTCCTCGCCTCCGCCTACGCCCACGAGCTCCCCCGCTACGGCCTCGAGGTCGGCCTCACCAACTACGCCGCCGCCTACTGCACCGGCCTCCTCCTCGCCCGCCGTGTCCTCAAGAAGCTCGAGATGGACGCTGAATATGAAGGGAACGTCGAGGCCACCGGCGAGGACTACTCCGTCGAGCCCGGCGAGAGCCGCAGGCCCTTCCGGGCTTTGCTCGATGTCGGCCTGGTGAAGACCACTACCGGAAACCGGGTTTTCGGAGCGCTGAAGGGAGCTTTGGATGGGGGTTTGGATGTGCCTCACAGTGAGAAGAGGTTCGCTGGGTTTTCGAAAGACTCGAAGCAGCTGGATGCCGAGGTTCACAGGAAGTATATCTATGGGGGCCATGTGGCGGCTTATATGGGGACTTTGATTGAGGATGAGCCGGAGAAGTACCAGACTCATTTCAGCGGGTACATCAAGAAGGGGATTGAGGCCGAGGGGATCGAGGAGCTGTACAAGAAGGTGCACGCCGCGATTCGGGCGGATCCTCTGGAGAAGAAGACTGCCAAGCCGGAGCCCAAGGCGCACAAGAGGTTCAACTTGAAGAAGCTGACTTATGAGGAGAGGAAGAACAAGTTGATCCAGAGGTTGAATGCCTTCAACTCGGCTGCTCAGGGCGATGAGGATTCGGATGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

314

Amino Acids

35.56

Weight (kDa)

9.45

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L5e PF17144 28 - 189 2.6e-81 Ribosomal large subunit proteins 60S L5, and 50S L18
Ribosomal_L18_c PF14204 249 - 302 2e-19 Ribosomal L18 C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 434
AbsI CCTCGAGG 1 cut(s) 305
AccII CGCG 4 cut(s) 72, 114, 135, 776
AclWI GGATC 5 cut(s) 12, 752, 782, 795, 877
AcuI CTGAAG 1 cut(s) 548
AfaI GTAC 6 cut(s) 101, 173, 188, 695, 716, 758
AfeI AGCGCT 2 cut(s) 86, 525
AfiI CCNNNNNNNGG 4 cut(s) 299, 510, 652, 780
AgsI TTSAA 6 cut(s) 63, 82, 841, 847, 895, 904
AjnI CCWGG 3 cut(s) 90, 156, 486
AjuI GAANNNNNNNTTGG 4 cut(s) 176, 208, 521, 553
AluBI AGCT 7 cut(s) 59, 289, 379, 536, 607, 754, 853
AluI AGCT 7 cut(s) 59, 289, 379, 536, 607, 754, 853
Alw21I GWGCWC 2 cut(s) 291, 771
Alw44I GTGCAC 1 cut(s) 767
AlwI GGATC 5 cut(s) 12, 752, 782, 795, 877
Ama87I CYCGRG 2 cut(s) 305, 380
Aor51HI AGCGCT 2 cut(s) 86, 525
AoxI GGCC 8 cut(s) 301, 313, 346, 414, 458, 484, 643, 735
ApaLI GTGCAC 1 cut(s) 767
ApeKI GCWGC 2 cut(s) 604, 911
Asp700I GAANNNNTTC 2 cut(s) 575, 899
AspLEI GCGC 6 cut(s) 74, 87, 108, 137, 526, 829
AspS9I GGNCC 2 cut(s) 459, 643
AsuC2I CCSGG 3 cut(s) 444, 467, 512
AsuHPI GGTGA 3 cut(s) 199, 310, 502
AsuII TTCGAA 1 cut(s) 590
AvaI CYCGRG 2 cut(s) 305, 380
BaeGI GKGCMC 1 cut(s) 771
BamHI GGATCC 2 cut(s) 4, 787
BanII GRGCYC 3 cut(s) 291, 444, 822
BauI CACGAG 1 cut(s) 284
BbsI GAAGAC 2 cut(s) 500, 809
Bbv12I GWGCWC 2 cut(s) 291, 771
BbvI GCAGC 2 cut(s) 616, 898
BccI CCATC 3 cut(s) 254, 379, 536
BceAI ACGGC 2 cut(s) 316, 348
BciT130I CCWGG 3 cut(s) 92, 158, 488
BcnI CCSGG 3 cut(s) 444, 467, 512
BfoI RGCGCY 3 cut(s) 88, 109, 527
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 9 cut(s) 109, 112, 330, 333, 454, 605, 654, 774, 912
BlsI GCNGC 9 cut(s) 110, 113, 331, 334, 455, 606, 655, 775, 913
Bme1390I CCNGG 6 cut(s) 92, 158, 444, 467, 488, 512
BmeT110I CYCGRG 2 cut(s) 305, 380
BmgT120I GGNCC 2 cut(s) 459, 643
BmiI GGNNCC 4 cut(s) 6, 644, 789, 819
BmrFI CCNGG 6 cut(s) 92, 158, 444, 467, 488, 512
BmsI GCATC 2 cut(s) 150, 601
BpiI GAAGAC 2 cut(s) 500, 809
BplI GAGNNNNNCTC 2 cut(s) 416, 448
BpmI CTGGAG 1 cut(s) 815
Bpu10I CCTNAGC 1 cut(s) 915
Bpu14I TTCGAA 1 cut(s) 590
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 3 cut(s) 444, 467, 512
BsaJI CCNNGG 3 cut(s) 615, 738, 822
BsaWI WCCGGW 1 cut(s) 503
Bsc4I CCNNNNNNNGG 4 cut(s) 299, 510, 652, 780
Bse118I RCCGGY 2 cut(s) 344, 419
BseBI CCWGG 3 cut(s) 92, 158, 488
BseDI CCNNGG 3 cut(s) 615, 738, 822
BseGI GGATG 6 cut(s) 141, 547, 559, 616, 685, 940
BseLI CCNNNNNNNGG 4 cut(s) 299, 510, 652, 780
BseMII CTCAG 1 cut(s) 929
BseRI GAGGAG 9 cut(s) 15, 18, 21, 24, 27, 341, 344, 764, 878
BseSI GKGCMC 1 cut(s) 771
BseXI GCAGC 2 cut(s) 616, 898
BseYI CCCAGC 1 cut(s) 581
BsgI GTGCAG 1 cut(s) 325
Bsh1236I CGCG 4 cut(s) 72, 114, 135, 776
BshFI GGCC 8 cut(s) 303, 315, 348, 416, 460, 486, 645, 737
BsiHKAI GWGCWC 2 cut(s) 291, 771
BsiHKCI CYCGRG 2 cut(s) 305, 380
BsiSI CCGG 9 cut(s) 252, 345, 420, 444, 466, 504, 511, 686, 815
BslFI GGGAC 1 cut(s) 679
BslI CCNNNNNNNGG 4 cut(s) 299, 510, 652, 780
BsmFI GGGAC 1 cut(s) 679
BsmI GAATGC 1 cut(s) 901
BsnI GGCC 8 cut(s) 303, 315, 348, 416, 460, 486, 645, 737
BsoBI CYCGRG 2 cut(s) 305, 380
Bsp119I TTCGAA 1 cut(s) 590
Bsp1286I GDGCHC 4 cut(s) 291, 444, 771, 822
Bsp1407I TGTACA 1 cut(s) 756
Bsp143I GATC 5 cut(s) 4, 231, 744, 787, 882
BspANI GGCC 8 cut(s) 303, 315, 348, 416, 460, 486, 645, 737
BspCNI CTCAG 1 cut(s) 928
BspFNI CGCG 4 cut(s) 72, 114, 135, 776
BspLI GGNNCC 4 cut(s) 6, 644, 789, 819
BspPI GGATC 5 cut(s) 12, 752, 782, 795, 877
BspT104I TTCGAA 1 cut(s) 590
BsrFI RCCGGY 2 cut(s) 344, 419
BsrGI TGTACA 1 cut(s) 756
BssAI RCCGGY 2 cut(s) 344, 419
BssECI CCNNGG 3 cut(s) 615, 738, 822
BssMI GATC 5 cut(s) 4, 231, 744, 787, 882
BssSI CACGAG 1 cut(s) 284
BssT1I CCWWGG 1 cut(s) 822
Bst2BI CACGAG 1 cut(s) 284
Bst2UI CCWGG 3 cut(s) 92, 158, 488
Bst4CI ACNGT 1 cut(s) 566
Bst6I CTCTTC 1 cut(s) 566
BstAUI TGTACA 1 cut(s) 756
BstBI TTCGAA 1 cut(s) 590
BstC8I GCNNGC 4 cut(s) 139, 361, 458, 771
BstDEI CTNAG 1 cut(s) 915
BstF5I GGATG 6 cut(s) 141, 547, 559, 616, 685, 940
BstFNI CGCG 4 cut(s) 72, 114, 135, 776
BstH2I RGCGCY 3 cut(s) 88, 109, 527
BstHHI GCGC 6 cut(s) 74, 87, 108, 137, 526, 829
BstKTI GATC 5 cut(s) 7, 234, 747, 790, 885
BstMBI GATC 5 cut(s) 4, 231, 744, 787, 882
BstMWI GCNNNNNNNGC 4 cut(s) 117, 453, 782, 826
BstNI CCWGG 3 cut(s) 92, 158, 488
BstSCI CCNGG 6 cut(s) 90, 156, 442, 465, 486, 510
BstSLI GKGCMC 1 cut(s) 771
BstUI CGCG 4 cut(s) 72, 114, 135, 776
BstV1I GCAGC 2 cut(s) 616, 898
BstV2I GAAGAC 2 cut(s) 500, 809
BstX2I RGATCY 2 cut(s) 4, 787
BstYI RGATCY 2 cut(s) 4, 787
BsuRI GGCC 8 cut(s) 303, 315, 348, 416, 460, 486, 645, 737
BtgZI GCGATG 1 cut(s) 936
BtsCI GGATG 6 cut(s) 141, 547, 559, 616, 685, 940
BtsIMutI CAGTG 1 cut(s) 571
Cac8I GCNNGC 4 cut(s) 139, 361, 458, 771
CfoI GCGC 6 cut(s) 74, 87, 108, 137, 526, 829
Cfr10I RCCGGY 2 cut(s) 344, 419
Cfr13I GGNCC 2 cut(s) 459, 643
CseI GACGC 1 cut(s) 398
Csp6I GTAC 6 cut(s) 100, 172, 187, 694, 715, 757
CviAII CATG 1 cut(s) 647
CviQI GTAC 6 cut(s) 100, 172, 187, 694, 715, 757
DdeI CTNAG 1 cut(s) 915
DpnI GATC 5 cut(s) 6, 233, 746, 789, 884
DpnII GATC 5 cut(s) 4, 231, 744, 787, 882
DrdI GACNNNNNNGTC 1 cut(s) 434
DseDI GACNNNNNNGTC 1 cut(s) 434
Eam1104I CTCTTC 1 cut(s) 566
EarI CTCTTC 1 cut(s) 566
EciI GGCGGA 4 cut(s) 134, 229, 262, 800
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 822
Eco24I GRGCYC 3 cut(s) 291, 444, 822
Eco47III AGCGCT 2 cut(s) 86, 525
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 1 cut(s) 548
Eco88I CYCGRG 2 cut(s) 305, 380
EcoICRI GAGCTC 1 cut(s) 289
EcoO109I RGGNCCY 1 cut(s) 459
EcoRII CCWGG 3 cut(s) 90, 156, 486
EcoT14I CCWWGG 1 cut(s) 822
EcoT38I GRGCYC 3 cut(s) 291, 444, 822
ErhI CCWWGG 1 cut(s) 822
FaeI CATG 1 cut(s) 650
FaiI YATR 7 cut(s) 399, 633, 639, 648, 660, 662, 861
FalI AAGNNNNNCTT 2 cut(s) 176, 208
FaqI GGGAC 1 cut(s) 679
FatI CATG 1 cut(s) 646
FauI CCCGC 5 cut(s) 77, 146, 302, 368, 704
Fnu4HI GCNGC 9 cut(s) 109, 112, 330, 333, 454, 605, 654, 774, 912
FokI GGATG 5 cut(s) 128, 554, 566, 623, 692
FriOI GRGCYC 3 cut(s) 291, 444, 822
Fsp4HI GCNGC 9 cut(s) 109, 112, 330, 333, 454, 605, 654, 774, 912
GlaI GCGC 6 cut(s) 73, 86, 107, 136, 525, 828
GluI GCNGC 9 cut(s) 109, 112, 330, 333, 454, 605, 654, 774, 912
GsaI CCCAGC 1 cut(s) 585
GsuI CTGGAG 1 cut(s) 815
HaeII RGCGCY 3 cut(s) 88, 109, 527
HaeIII GGCC 8 cut(s) 303, 315, 348, 416, 460, 486, 645, 737
HapII CCGG 9 cut(s) 252, 345, 420, 444, 466, 504, 511, 686, 815
HgaI GACGC 1 cut(s) 398
HhaI GCGC 6 cut(s) 74, 87, 108, 137, 526, 829
Hin1II CATG 1 cut(s) 650
Hin6I GCGC 6 cut(s) 72, 85, 106, 135, 524, 827
HinP1I GCGC 6 cut(s) 72, 85, 106, 135, 524, 827
HindIII AAGCTT 1 cut(s) 57
HinfI GANTC 4 cut(s) 596, 700, 778, 929
HpaII CCGG 9 cut(s) 252, 345, 420, 444, 466, 504, 511, 686, 815
HphI GGTGA 3 cut(s) 199, 310, 502
Hpy166II GTNNAC 2 cut(s) 622, 769
Hpy188I TCNGA 2 cut(s) 521, 934
Hpy188III TCNNGA 5 cut(s) 373, 382, 721, 794, 886
Hpy8I GTNNAC 2 cut(s) 622, 769
Hpy99I CGWCG 4 cut(s) 200, 227, 413, 440
HpyAV CCTTC 7 cut(s) 110, 395, 472, 523, 718, 757, 910
HpyCH4III ACNGT 1 cut(s) 566
HpyCH4IV ACGT 1 cut(s) 408
HpyCH4V TGCA 2 cut(s) 342, 769
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 453, 782, 826
HpyF3I CTNAG 1 cut(s) 915
HpySE526I ACGT 1 cut(s) 408
Hsp92II CATG 1 cut(s) 650
HspAI GCGC 6 cut(s) 72, 85, 106, 135, 524, 827
Kzo9I GATC 5 cut(s) 4, 231, 744, 787, 882
LmnI GCTCC 5 cut(s) 294, 521, 533, 751, 817
Lsp1109I GCAGC 2 cut(s) 616, 898
LweI GCATC 2 cut(s) 150, 601
MaeII ACGT 1 cut(s) 408
MalI GATC 5 cut(s) 6, 233, 746, 789, 884
MboI GATC 5 cut(s) 4, 231, 744, 787, 882
MboII GAAGA 6 cut(s) 505, 583, 811, 814, 859, 883
MflI RGATCY 2 cut(s) 4, 787
MhlI GDGCHC 4 cut(s) 291, 444, 771, 822
MlyI GAGTC 2 cut(s) 590, 694
MroXI GAANNNNTTC 2 cut(s) 575, 899
MspA1I CMGCKG 2 cut(s) 607, 711
MspI CCGG 9 cut(s) 252, 345, 420, 444, 466, 504, 511, 686, 815
MspR9I CCNGG 6 cut(s) 92, 158, 444, 467, 488, 512
Mva1269I GAATGC 1 cut(s) 901
MvaI CCWGG 3 cut(s) 92, 158, 488
MvnI CGCG 4 cut(s) 72, 114, 135, 776
MwoI GCNNNNNNNGC 4 cut(s) 117, 453, 782, 826
NciI CCSGG 3 cut(s) 444, 467, 512
NdeII GATC 5 cut(s) 4, 231, 744, 787, 882
NlaIII CATG 1 cut(s) 650
NlaIV GGNNCC 4 cut(s) 6, 644, 789, 819
NmeAIII GCCGAG 3 cut(s) 640, 763, 887
NspV TTCGAA 1 cut(s) 590
PaeR7I CTCGAG 2 cut(s) 305, 380
PcsI WCGNNNNNNNCGW 2 cut(s) 444, 596
PctI GAATGC 1 cut(s) 901
PdmI GAANNNNTTC 2 cut(s) 575, 899
PfeI GAWTC 2 cut(s) 778, 929
PflFI GACNNNGTC 2 cut(s) 221, 260
PkrI GCNGC 9 cut(s) 110, 113, 331, 334, 455, 606, 655, 775, 913
PleI GAGTC 2 cut(s) 590, 694
PpsI GAGTC 2 cut(s) 590, 694
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 3 cut(s) 90, 156, 486
PspFI CCCAGC 1 cut(s) 581
PspGI CCWGG 3 cut(s) 90, 156, 486
PspN4I GGNNCC 4 cut(s) 6, 644, 789, 819
PspPI GGNCC 2 cut(s) 459, 643
PspXI VCTCGAGB 1 cut(s) 305
PsuI RGATCY 2 cut(s) 4, 787
PsyI GACNNNGTC 2 cut(s) 221, 260
PvuII CAGCTG 1 cut(s) 607
RsaI GTAC 6 cut(s) 101, 173, 188, 695, 716, 758
RsaNI GTAC 6 cut(s) 100, 172, 187, 694, 715, 757
SacI GAGCTC 1 cut(s) 291
SatI GCNGC 9 cut(s) 109, 112, 330, 333, 454, 605, 654, 774, 912
Sau3AI GATC 5 cut(s) 4, 231, 744, 787, 882
Sau96I GGNCC 2 cut(s) 459, 643
SchI GAGTC 2 cut(s) 590, 694
ScrFI CCNGG 6 cut(s) 92, 158, 444, 467, 488, 512
SduI GDGCHC 4 cut(s) 291, 444, 771, 822
SfaNI GCATC 2 cut(s) 150, 601
Sfr274I CTCGAG 2 cut(s) 305, 380
SfuI TTCGAA 1 cut(s) 590
SgrAI CRCCGGYG 1 cut(s) 419
SlaI CTCGAG 2 cut(s) 305, 380
SmlI CTYRAG 3 cut(s) 305, 371, 380
SmoI CTYRAG 3 cut(s) 305, 371, 380
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 6 cut(s) 90, 156, 442, 465, 486, 510
StyI CCWWGG 1 cut(s) 822
TaaI ACNGT 1 cut(s) 566
TaiI ACGT 1 cut(s) 411
TaqI TCGA 8 cut(s) 306, 381, 411, 438, 477, 590, 599, 747
TaqII GACCGA 1 cut(s) 140
TatI WGTACW 3 cut(s) 99, 171, 756
TauI GCSGC 7 cut(s) 111, 114, 332, 335, 456, 656, 776
TfiI GAWTC 2 cut(s) 778, 929
TscAI CASTG 1 cut(s) 571
TseI GCWGC 2 cut(s) 604, 911
TspDTI ATGAA 2 cut(s) 40, 414
TspGWI ACGGA 1 cut(s) 424
TspRI CASTG 1 cut(s) 571
Tth111I GACNNNGTC 2 cut(s) 221, 260
VneI GTGCAC 1 cut(s) 767
XhoI CTCGAG 2 cut(s) 305, 380
XmnI GAANNNNTTC 2 cut(s) 575, 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.