MD02G1004200.v1.1

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001819
Physical Location & Seq
Reverse (-)
290249 .. 293300
3052 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1004200.v1.1.491

Sequence Viewer

Length: 909 bp
ATGCCGTTCGTCAAGTCTCAGAAGTCTAGGGCTTACTTCAAGAGGTTTCAAGTCAAGTATAAGAGAAGGAGAGAGGGGAAGACCGACTACCGTGCCAGGATTAGGCTCATTAACCAAGACAAGAACAAGTACAACACACCCAAGTATCGGTTCGTTGTGCGATTTACCAACAAGGATATTGTTGCCCAGATTGTATCTGCCAGCATTGCTGGCGATTTGGTTCTCGCTGCAGCCTATGCCCATGAGCTGCCACACTATGGACTTGAAGTTGGTCTGACAAACTATGCAGCAGCCTACTGCACTGGCCTACTCTTGGCTCGTCGTGTGCTGAAGAAGCTTGAAATGGATGACGAATATGAGGGAAATGTTGAGGCAACTGGTGAGGATTATTCTGTAGAGCCAGCTGAGAGCAGGAGGCCATTCCGTGCTCTTCTTGATGTTGGCCTGATCAGGACCACCACAGGAAATCGTGTATTCGGTGCCTTGAAGGGTGCTTTGGATGGGGGTTTGGATATCCCTCACAGTGAAAAGAGGTTTGCCGGTTTCTCCAAGGATAGCAAGCAACTTGACGCTGAGGTTCATCGCAAGTACATCTATGGTGGCCATGTTGCTGCATACATGACAACTTTGGCGGATGATGAGCCAGAGAAATTCCAGAGTCACTTCAGTGAATATATTAAGAAAGGTATTGAAGCGGATAACATCGAGGAGCTGTACAAGAAAGTTCATGCTGCCATCCGTGCTGATCCTACAATTAAGAAGACTGAGAAGCCGGCCCCTCAAGAGCACAAGAGGTACAACCTCAAGAAGCTTACCTTTGACGAGAGGAAGAAGAAGTTGGTTGAGAGGTTGAAGGCCTTCAATGAAGCTGGTAACGATGAAAACAGTGACGATGATGATGAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

303

Amino Acids

34.64

Weight (kDa)

9.17

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L5e PF17144 14 - 175 3.2e-82 Ribosomal large subunit proteins 60S L5, and 50S L18
Ribosomal_L18_c PF14204 235 - 288 3.1e-18 Ribosomal L18 C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 479
AccB7I CCANNNNNTGG 1 cut(s) 257
AciI CCGC 2 cut(s) 632, 695
AclWI GGATC 1 cut(s) 740
AcoI YGGCCR 1 cut(s) 601
AcsI RAATTY 1 cut(s) 650
AcuI CTGAAG 2 cut(s) 350, 649
AfaI GTAC 5 cut(s) 131, 590, 716, 797, 905
AfiI CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
AgsI TTSAA 8 cut(s) 40, 50, 266, 341, 487, 692, 853, 862
AjnI CCWGG 1 cut(s) 95
AjuI GAANNNNNNNTTGG 6 cut(s) 134, 166, 479, 511, 821, 853
AleI CACNNNNGTG 1 cut(s) 666
AluBI AGCT 6 cut(s) 247, 337, 404, 712, 811, 869
AluI AGCT 6 cut(s) 247, 337, 404, 712, 811, 869
Alw21I GWGCWC 2 cut(s) 430, 789
Alw26I GTCTC 1 cut(s) 21
AlwI GGATC 1 cut(s) 740
AoxI GGCC 6 cut(s) 304, 416, 442, 601, 774, 855
ApeKI GCWGC 7 cut(s) 227, 230, 247, 287, 290, 611, 731
ApoI RAATTY 1 cut(s) 650
Asp700I GAANNNNTTC 1 cut(s) 857
AspS9I GGNCC 2 cut(s) 453, 775
AsuHPI GGTGA 1 cut(s) 392
AvaII GGWCC 1 cut(s) 453
BaeI ACNNNNGTAYC 2 cut(s) 128, 161
BalI TGGCCA 1 cut(s) 603
BanI GGYRCC 1 cut(s) 479
BarI GAAGNNNNNNTAC 2 cut(s) 71, 103
BbsI GAAGAC 2 cut(s) 86, 767
Bbv12I GWGCWC 2 cut(s) 430, 789
BbvCI CCTCAGC 1 cut(s) 573
BbvI GCAGC 7 cut(s) 214, 234, 242, 299, 302, 598, 718
BccI CCATC 2 cut(s) 494, 743
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BciT130I CCWGG 1 cut(s) 97
BclI TGATCA 1 cut(s) 447
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 1 cut(s) 27
BfmI CTRYAG 2 cut(s) 228, 393
BisI GCNGC 7 cut(s) 228, 231, 248, 288, 291, 612, 732
BlsI GCNGC 7 cut(s) 229, 232, 249, 289, 292, 613, 733
BmcAI AGTACT 1 cut(s) 905
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 453
BmgT120I GGNCC 2 cut(s) 453, 775
BmiI GGNNCC 2 cut(s) 481, 777
BmrFI CCNGG 1 cut(s) 97
BpiI GAAGAC 2 cut(s) 86, 767
Bpu10I CCTNAGC 1 cut(s) 573
BpuEI CTTGAG 2 cut(s) 765, 788
BsaJI CCNNGG 1 cut(s) 549
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
Bse118I RCCGGY 2 cut(s) 539, 772
Bse1I ACTGG 2 cut(s) 307, 382
Bse3DI GCAATG 1 cut(s) 204
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 549
BseGI GGATG 4 cut(s) 352, 505, 640, 735
BseLI CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 4 cut(s) 32, 396, 564, 756
BseNI ACTGG 2 cut(s) 307, 382
BseRI GAGGAG 1 cut(s) 722
BseXI GCAGC 7 cut(s) 214, 234, 242, 299, 302, 598, 718
BsgI GTGCAG 1 cut(s) 283
BshFI GGCC 6 cut(s) 306, 418, 444, 603, 776, 857
BshNI GGYRCC 1 cut(s) 479
BsiHKAI GWGCWC 2 cut(s) 430, 789
BsiSI CCGG 2 cut(s) 540, 773
BslI CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
BsmAI GTCTC 1 cut(s) 21
BsnI GGCC 6 cut(s) 306, 418, 444, 603, 776, 857
Bsp1286I GDGCHC 2 cut(s) 430, 789
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 2 cut(s) 447, 745
BspACI CCGC 2 cut(s) 632, 695
BspANI GGCC 6 cut(s) 306, 418, 444, 603, 776, 857
BspCNI CTCAG 4 cut(s) 31, 397, 565, 757
BspLI GGNNCC 2 cut(s) 481, 777
BspMAI CTGCAG 1 cut(s) 232
BspPI GGATC 1 cut(s) 740
BspQI GCTCTTC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 479
BsrDI GCAATG 1 cut(s) 204
BsrFI RCCGGY 2 cut(s) 539, 772
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 2 cut(s) 307, 382
BssAI RCCGGY 2 cut(s) 539, 772
BssECI CCNNGG 1 cut(s) 549
BssMI GATC 2 cut(s) 447, 745
BssT1I CCWWGG 1 cut(s) 549
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 3 cut(s) 92, 524, 887
Bst6I CTCTTC 1 cut(s) 435
BstAPI GCANNNNNTGC 1 cut(s) 236
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 5 cut(s) 202, 211, 402, 560, 774
BstDEI CTNAG 4 cut(s) 18, 405, 573, 765
BstF5I GGATG 4 cut(s) 352, 505, 640, 735
BstKTI GATC 2 cut(s) 450, 748
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 2 cut(s) 447, 745
BstMWI GCNNNNNNNGC 5 cut(s) 206, 210, 236, 334, 740
BstNI CCWGG 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 95
BstSFI CTRYAG 2 cut(s) 228, 393
BstV1I GCAGC 7 cut(s) 214, 234, 242, 299, 302, 598, 718
BstV2I GAAGAC 2 cut(s) 86, 767
BsuRI GGCC 6 cut(s) 306, 418, 444, 603, 776, 857
BtgZI GCGATG 1 cut(s) 566
BtsCI GGATG 4 cut(s) 352, 505, 640, 735
BtsIMutI CAGTG 4 cut(s) 300, 529, 673, 892
Cac8I GCNNGC 5 cut(s) 202, 211, 402, 560, 774
Cfr10I RCCGGY 2 cut(s) 539, 772
Cfr13I GGNCC 2 cut(s) 453, 775
CseI GACGC 1 cut(s) 578
Csp6I GTAC 5 cut(s) 130, 589, 715, 796, 904
CviAII CATG 4 cut(s) 242, 605, 619, 728
CviQI GTAC 5 cut(s) 130, 589, 715, 796, 904
DdeI CTNAG 4 cut(s) 18, 405, 573, 765
DpnI GATC 2 cut(s) 449, 747
DpnII GATC 2 cut(s) 447, 745
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
EciI GGCGGA 1 cut(s) 647
Eco130I CCWWGG 1 cut(s) 549
Eco147I AGGCCT 1 cut(s) 857
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 453
Eco57I CTGAAG 2 cut(s) 350, 649
EcoRII CCWGG 1 cut(s) 95
EcoRV GATATC 1 cut(s) 514
EcoT14I CCWWGG 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 549
FaeI CATG 4 cut(s) 245, 608, 622, 731
FalI AAGNNNNNCTT 2 cut(s) 800, 832
FatI CATG 4 cut(s) 241, 604, 618, 727
FbaI TGATCA 1 cut(s) 447
Fnu4HI GCNGC 7 cut(s) 228, 231, 248, 288, 291, 612, 732
FokI GGATG 4 cut(s) 359, 512, 647, 722
Fsp4HI GCNGC 7 cut(s) 228, 231, 248, 288, 291, 612, 732
FspBI CTAG 1 cut(s) 27
GluI GCNGC 7 cut(s) 228, 231, 248, 288, 291, 612, 732
HaeIII GGCC 6 cut(s) 306, 418, 444, 603, 776, 857
HapII CCGG 2 cut(s) 540, 773
HgaI GACGC 1 cut(s) 578
Hin1II CATG 4 cut(s) 245, 608, 622, 731
HindIII AAGCTT 2 cut(s) 335, 809
HinfI GANTC 1 cut(s) 658
HpaII CCGG 2 cut(s) 540, 773
HphI GGTGA 1 cut(s) 392
Hpy188I TCNGA 2 cut(s) 21, 276
Hpy188III TCNNGA 6 cut(s) 40, 434, 451, 655, 782, 805
Hpy99I CGWCG 1 cut(s) 324
HpyAV CCTTC 4 cut(s) 60, 481, 847, 868
HpyCH4III ACNGT 3 cut(s) 92, 524, 887
HpyCH4V TGCA 4 cut(s) 230, 287, 300, 614
HpyF10VI GCNNNNNNNGC 5 cut(s) 206, 210, 236, 334, 740
HpyF3I CTNAG 4 cut(s) 18, 405, 573, 765
Hsp92II CATG 4 cut(s) 245, 608, 622, 731
KroI GCCGGC 1 cut(s) 772
KroNI GCCGGC 1 cut(s) 774
Ksp22I TGATCA 1 cut(s) 447
Kzo9I GATC 2 cut(s) 447, 745
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 1 cut(s) 709
Lsp1109I GCAGC 7 cut(s) 214, 234, 242, 299, 302, 598, 718
MaeI CTAG 1 cut(s) 27
MaeIII GTNAC 3 cut(s) 659, 872, 887
MalI GATC 2 cut(s) 449, 747
MboI GATC 2 cut(s) 447, 745
MboII GAAGA 6 cut(s) 91, 343, 422, 772, 841, 844
MhlI GDGCHC 2 cut(s) 430, 789
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 2 cut(s) 650, 753
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 1 cut(s) 667
Mox20I TGGCCA 1 cut(s) 603
MroNI GCCGGC 1 cut(s) 772
MroXI GAANNNNTTC 1 cut(s) 857
MscI TGGCCA 1 cut(s) 603
MseI TTAA 3 cut(s) 111, 678, 756
MslI CAYNNNNRTG 1 cut(s) 666
Msp20I TGGCCA 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 2 cut(s) 540, 773
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 5 cut(s) 206, 210, 236, 334, 740
NaeI GCCGGC 1 cut(s) 774
NdeII GATC 2 cut(s) 447, 745
NgoMIV GCCGGC 1 cut(s) 772
NlaIII CATG 4 cut(s) 245, 608, 622, 731
NlaIV GGNNCC 2 cut(s) 481, 777
NmuCI GTSAC 2 cut(s) 659, 887
OliI CACNNNNGTG 1 cut(s) 666
PceI AGGCCT 1 cut(s) 857
PciSI GCTCTTC 1 cut(s) 435
PdiI GCCGGC 1 cut(s) 774
PdmI GAANNNNTTC 1 cut(s) 857
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 7 cut(s) 229, 232, 249, 289, 292, 613, 733
PleI GAGTC 1 cut(s) 666
PpsI GAGTC 1 cut(s) 666
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 2 cut(s) 481, 777
PspPI GGNCC 2 cut(s) 453, 775
PstI CTGCAG 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 5 cut(s) 131, 590, 716, 797, 905
RsaNI GTAC 5 cut(s) 130, 589, 715, 796, 904
RseI CAYNNNNRTG 1 cut(s) 666
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 3 cut(s) 111, 678, 756
SatI GCNGC 7 cut(s) 228, 231, 248, 288, 291, 612, 732
Sau3AI GATC 2 cut(s) 447, 745
Sau96I GGNCC 2 cut(s) 453, 775
ScaI AGTACT 1 cut(s) 905
SchI GAGTC 1 cut(s) 667
ScrFI CCNGG 1 cut(s) 97
SduI GDGCHC 2 cut(s) 430, 789
SfcI CTRYAG 2 cut(s) 228, 393
SinI GGWCC 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 2 cut(s) 780, 803
SmoI CTYRAG 2 cut(s) 780, 803
Sse9I AATT 2 cut(s) 650, 753
SseBI AGGCCT 1 cut(s) 857
SsiI CCGC 2 cut(s) 632, 695
SspMI CTAG 1 cut(s) 27
StuI AGGCCT 1 cut(s) 857
StyD4I CCNGG 1 cut(s) 95
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 3 cut(s) 92, 524, 887
TaqI TCGA 1 cut(s) 705
TaqII GACCGA 1 cut(s) 98
TasI AATT 2 cut(s) 650, 753
TatI WGTACW 4 cut(s) 129, 588, 714, 903
Tru1I TTAA 3 cut(s) 111, 678, 756
Tru9I TTAA 3 cut(s) 111, 678, 756
TscAI CASTG 4 cut(s) 307, 529, 673, 892
TseFI GTSAC 2 cut(s) 659, 887
TseI GCWGC 7 cut(s) 227, 230, 247, 287, 290, 611, 731
Tsp45I GTSAC 2 cut(s) 659, 887
TspDTI ATGAA 4 cut(s) 569, 716, 879, 894
TspGWI ACGGA 2 cut(s) 413, 728
TspRI CASTG 4 cut(s) 307, 529, 673, 892
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 453
XapI RAATTY 1 cut(s) 650
XmnI GAANNNNTTC 1 cut(s) 857
XspI CTAG 1 cut(s) 27
ZrmI AGTACT 1 cut(s) 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.