FvH4_4g03590

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001819
Physical Location & Seq
Reverse (-)
3225770 .. 3227395
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g03590.t1

Sequence Viewer

Length: 996 bp
ATGAAGAGCGCGCCCGTCTTGCTGAGGAATCGTAAGAATATGCAAATGATGCAATATCGCCGCCTGCCTACTCTCTCTCTCTGCAGAGCAGCAATGGCGTTCATCAAGGCCTCCAAGCCCCGCGCCTACTTCAAGCGCTTCCAGGTCAAATACAAGCGCCGCCGTGAGGGCAAGACCGACTACCGCGCCCGCATCCGTCTCATCAACCAGGACAAGAACAAGTACAACACCCCCAAGTACCGCTTCGTCGTCCGCTTCACCAACAAGGACATCGTCGCTCAGATCATCTCCTCCACCATCTCCGGCGACCTCGTCCTCGCCTCCGCCTACTCCCACGAGCTCCCCCGCTACGGCCTCCACGTCGGCCTCACCAACTACGCCGCCTCCTACTGCACCGGCCTCCTCCTCGCCCGCCGCACTCTGAAGAAGCTCGAGATGGACGAGGAGTACGAGGGAAACCTCGAGGCCACCGGCGAGGACTTCTCCGTCGAGCCCGGCGAGAGCCGCAGGCCCTTCCGTGCTCTTCTTGATGTCGGACTTGTCCGCACCACTACCGGAAACCGCGTCTTCGGCGCGCTGAAGGGAGCTCTGGACGGCGGGATCGACGTGCCGCACAGTGAGAAGAGGTTCGCTGGATTCTCCAAGGACTCGAAGAGCTTGGATGCCGAGACGCACAGGAAGTACATCTATGGAGGACACGTCGCGGCTTACATGGGGACTTTGATTGAGGATGAGCCGGAGAAGTACCAGACTCACTTCAGCGAGTACATCAAGAAGGGGATTGAGGCTGAGGGGATTGAGGAGCTGTACAAGAAGGTGCATGCTGCGATTCGGGCGGATCCTCTGGAGAAGAAGACTGCCAAGCCGGAGCCCAAGCAGCACAAGAGGTTCAACTTGAAGAAGCTGACTTATGAGGAGAGGAAGAACAAGTTGATCGAGAGGTTGAATGCCTTCAACTCGGCTGCTCAAGGGGATGAGGACTCTGATGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

332

Amino Acids

37.99

Weight (kDa)

9.63

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L5e PF17144 45 - 206 1.4e-80 Ribosomal large subunit proteins 60S L5, and 50S L18
Ribosomal_L18_c PF14204 266 - 319 7.9e-19 Ribosomal L18 C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 485
AbsI CCTCGAGG 1 cut(s) 461
AccII CGCG 6 cut(s) 11, 123, 186, 564, 575, 704
AclWI GGATC 3 cut(s) 608, 833, 846
AcuI CTGAAG 3 cut(s) 443, 599, 742
AfaI GTAC 7 cut(s) 224, 239, 449, 683, 746, 767, 809
AfeI AGCGCT 1 cut(s) 137
AfiI CCNNNNNNNGG 2 cut(s) 166, 350
AflIII ACRYGT 1 cut(s) 697
AgsI TTSAA 5 cut(s) 133, 892, 898, 946, 955
AjiI CACGTC 3 cut(s) 361, 607, 700
AjnI CCWGG 2 cut(s) 141, 207
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AluBI AGCT 6 cut(s) 340, 430, 587, 657, 805, 904
AluI AGCT 6 cut(s) 340, 430, 587, 657, 805, 904
Alw21I GWGCWC 3 cut(s) 342, 523, 589
Alw26I GTCTC 2 cut(s) 203, 662
AlwI GGATC 3 cut(s) 608, 833, 846
Ama87I CYCGRG 2 cut(s) 431, 461
Aor51HI AGCGCT 1 cut(s) 137
AoxI GGCC 6 cut(s) 108, 352, 364, 397, 465, 509
ApeKI GCWGC 4 cut(s) 89, 824, 877, 962
Asp700I GAANNNNTTC 2 cut(s) 626, 950
AspLEI GCGC 8 cut(s) 11, 13, 125, 138, 159, 188, 575, 577
AspS9I GGNCC 1 cut(s) 510
AsuC2I CCSGG 1 cut(s) 495
AsuHPI GGTGA 2 cut(s) 250, 361
AvaI CYCGRG 2 cut(s) 431, 461
BamHI GGATCC 1 cut(s) 838
BanII GRGCYC 4 cut(s) 342, 495, 589, 873
BauI CACGAG 1 cut(s) 335
BbsI GAAGAC 2 cut(s) 559, 860
Bbv12I GWGCWC 3 cut(s) 342, 523, 589
BbvCI CCTCAGC 2 cut(s) 23, 789
BbvI GCAGC 4 cut(s) 101, 811, 889, 949
BccI CCATC 2 cut(s) 305, 430
BceAI ACGGC 3 cut(s) 147, 367, 610
BciT130I CCWGG 2 cut(s) 143, 209
BcnI CCSGG 1 cut(s) 495
BcoDI GTCTC 2 cut(s) 203, 662
BfmI CTRYAG 1 cut(s) 82
BfoI RGCGCY 2 cut(s) 139, 160
BglI GCCNNNNNGGC 1 cut(s) 168
Bme1390I CCNGG 3 cut(s) 143, 209, 495
BmeT110I CYCGRG 2 cut(s) 431, 461
BmgBI CACGTC 3 cut(s) 361, 607, 700
BmgT120I GGNCC 1 cut(s) 510
BmiI GGNNCC 2 cut(s) 840, 870
BmrFI CCNGG 3 cut(s) 143, 209, 495
BmsI GCATC 3 cut(s) 39, 201, 652
BpiI GAAGAC 2 cut(s) 559, 860
BplI GAGNNNNNCTC 2 cut(s) 467, 499
BpmI CTGGAG 1 cut(s) 866
Bpu10I CCTNAGC 2 cut(s) 23, 789
BpuEI CTTGAG 1 cut(s) 951
BpuMI CCSGG 1 cut(s) 495
BsaJI CCNNGG 1 cut(s) 642
BsaWI WCCGGW 1 cut(s) 554
BsaXI ACNNNNNCTCC 4 cut(s) 368, 398, 684, 714
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 350
Bse118I RCCGGY 2 cut(s) 395, 470
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 2 cut(s) 143, 209
BseDI CCNNGG 1 cut(s) 642
BseGI GGATG 4 cut(s) 192, 667, 736, 979
BseLI CCNNNNNNNGG 2 cut(s) 166, 350
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 3 cut(s) 14, 293, 780
BsePI GCGCGC 2 cut(s) 9, 573
BseRI GAGGAG 6 cut(s) 280, 392, 395, 458, 815, 929
BseXI GCAGC 4 cut(s) 101, 811, 889, 949
BsgI GTGCAG 1 cut(s) 376
Bsh1236I CGCG 6 cut(s) 11, 123, 186, 564, 575, 704
BshFI GGCC 6 cut(s) 110, 354, 366, 399, 467, 511
BsiHKAI GWGCWC 3 cut(s) 342, 523, 589
BsiHKCI CYCGRG 2 cut(s) 431, 461
BsiSI CCGG 7 cut(s) 303, 396, 471, 495, 555, 737, 866
BslFI GGGAC 1 cut(s) 730
BslI CCNNNNNNNGG 2 cut(s) 166, 350
BsmAI GTCTC 2 cut(s) 203, 662
BsmBI CGTCTC 2 cut(s) 203, 662
BsmFI GGGAC 1 cut(s) 730
BsmI GAATGC 1 cut(s) 952
BsnI GGCC 6 cut(s) 110, 354, 366, 399, 467, 511
BsoBI CYCGRG 2 cut(s) 431, 461
Bsp1286I GDGCHC 5 cut(s) 342, 495, 523, 589, 873
Bsp1407I TGTACA 1 cut(s) 807
Bsp143I GATC 4 cut(s) 282, 600, 838, 933
BspANI GGCC 6 cut(s) 110, 354, 366, 399, 467, 511
BspCNI CTCAG 3 cut(s) 15, 292, 781
BspFNI CGCG 6 cut(s) 11, 123, 186, 564, 575, 704
BspLI GGNNCC 2 cut(s) 840, 870
BspMAI CTGCAG 1 cut(s) 86
BspPI GGATC 3 cut(s) 608, 833, 846
BspQI GCTCTTC 2 cut(s) 528, 647
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 2 cut(s) 395, 470
BsrGI TGTACA 1 cut(s) 807
BssAI RCCGGY 2 cut(s) 395, 470
BssECI CCNNGG 1 cut(s) 642
BssHII GCGCGC 2 cut(s) 9, 573
BssMI GATC 4 cut(s) 282, 600, 838, 933
BssSI CACGAG 1 cut(s) 335
BssT1I CCWWGG 1 cut(s) 642
Bst2BI CACGAG 1 cut(s) 335
Bst2UI CCWGG 2 cut(s) 143, 209
Bst4CI ACNGT 1 cut(s) 617
Bst6I CTCTTC 3 cut(s) 528, 617, 647
BstAPI GCANNNNNTGC 1 cut(s) 49
BstAUI TGTACA 1 cut(s) 807
BstC8I GCNNGC 7 cut(s) 11, 65, 190, 412, 509, 575, 822
BstDEI CTNAG 3 cut(s) 23, 279, 789
BstF5I GGATG 4 cut(s) 192, 667, 736, 979
BstFNI CGCG 6 cut(s) 11, 123, 186, 564, 575, 704
BstH2I RGCGCY 2 cut(s) 139, 160
BstHHI GCGC 8 cut(s) 11, 13, 125, 138, 159, 188, 575, 577
BstKTI GATC 4 cut(s) 285, 603, 841, 936
BstMAI GTCTC 2 cut(s) 203, 662
BstMBI GATC 4 cut(s) 282, 600, 838, 933
BstMWI GCNNNNNNNGC 8 cut(s) 19, 49, 95, 168, 504, 570, 833, 877
BstNI CCWGG 2 cut(s) 143, 209
BstNSI RCATGY 1 cut(s) 824
BstSCI CCNGG 3 cut(s) 141, 207, 493
BstSFI CTRYAG 1 cut(s) 82
BstUI CGCG 6 cut(s) 11, 123, 186, 564, 575, 704
BstV1I GCAGC 4 cut(s) 101, 811, 889, 949
BstV2I GAAGAC 2 cut(s) 559, 860
BstX2I RGATCY 1 cut(s) 838
BstYI RGATCY 1 cut(s) 838
BsuRI GGCC 6 cut(s) 110, 354, 366, 399, 467, 511
BtrI CACGTC 3 cut(s) 361, 607, 700
BtsCI GGATG 4 cut(s) 192, 667, 736, 979
BtsIMutI CAGTG 1 cut(s) 622
Cac8I GCNNGC 7 cut(s) 11, 65, 190, 412, 509, 575, 822
CfoI GCGC 8 cut(s) 11, 13, 125, 138, 159, 188, 575, 577
Cfr10I RCCGGY 2 cut(s) 395, 470
Cfr13I GGNCC 1 cut(s) 510
CseI GACGC 2 cut(s) 553, 679
Csp6I GTAC 7 cut(s) 223, 238, 448, 682, 745, 766, 808
CviAII CATG 2 cut(s) 712, 821
CviQI GTAC 7 cut(s) 223, 238, 448, 682, 745, 766, 808
DdeI CTNAG 3 cut(s) 23, 279, 789
DpnI GATC 4 cut(s) 284, 602, 840, 935
DpnII GATC 4 cut(s) 282, 600, 838, 933
DrdI GACNNNNNNGTC 1 cut(s) 485
DseDI GACNNNNNNGTC 1 cut(s) 485
Eam1104I CTCTTC 3 cut(s) 528, 617, 647
EarI CTCTTC 3 cut(s) 528, 617, 647
EciI GGCGGA 2 cut(s) 313, 851
Ecl136II GAGCTC 2 cut(s) 340, 587
Eco130I CCWWGG 1 cut(s) 642
Eco147I AGGCCT 1 cut(s) 110
Eco24I GRGCYC 4 cut(s) 342, 495, 589, 873
Eco47III AGCGCT 1 cut(s) 137
Eco53kI GAGCTC 2 cut(s) 340, 587
Eco57I CTGAAG 3 cut(s) 443, 599, 742
Eco88I CYCGRG 2 cut(s) 431, 461
EcoICRI GAGCTC 2 cut(s) 340, 587
EcoO109I RGGNCCY 1 cut(s) 510
EcoRII CCWGG 2 cut(s) 141, 207
EcoT14I CCWWGG 1 cut(s) 642
EcoT38I GRGCYC 4 cut(s) 342, 495, 589, 873
ErhI CCWWGG 1 cut(s) 642
Esp3I CGTCTC 2 cut(s) 203, 662
FaeI CATG 2 cut(s) 715, 824
FaiI YATR 5 cut(s) 41, 690, 713, 822, 912
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FaqI GGGAC 1 cut(s) 730
FatI CATG 2 cut(s) 711, 820
FauI CCCGC 5 cut(s) 128, 197, 353, 419, 590
FokI GGATG 4 cut(s) 179, 674, 743, 986
FriOI GRGCYC 4 cut(s) 342, 495, 589, 873
GlaI GCGC 8 cut(s) 10, 12, 124, 137, 158, 187, 574, 576
GsuI CTGGAG 1 cut(s) 866
HaeII RGCGCY 2 cut(s) 139, 160
HaeIII GGCC 6 cut(s) 110, 354, 366, 399, 467, 511
HapII CCGG 7 cut(s) 303, 396, 471, 495, 555, 737, 866
HgaI GACGC 2 cut(s) 553, 679
HhaI GCGC 8 cut(s) 11, 13, 125, 138, 159, 188, 575, 577
Hin1II CATG 2 cut(s) 715, 824
Hin6I GCGC 8 cut(s) 9, 11, 123, 136, 157, 186, 573, 575
HinP1I GCGC 8 cut(s) 9, 11, 123, 136, 157, 186, 573, 575
HinfI GANTC 6 cut(s) 28, 636, 647, 751, 829, 980
HpaII CCGG 7 cut(s) 303, 396, 471, 495, 555, 737, 866
HphI GGTGA 2 cut(s) 250, 361
Hpy188I TCNGA 4 cut(s) 282, 423, 536, 985
Hpy188III TCNNGA 6 cut(s) 433, 527, 590, 772, 845, 937
Hpy99I CGWCG 6 cut(s) 251, 278, 365, 491, 608, 704
HpyAV CCTTC 5 cut(s) 523, 574, 769, 808, 961
HpyCH4III ACNGT 1 cut(s) 617
HpyCH4IV ACGT 3 cut(s) 360, 606, 699
HpyCH4V TGCA 5 cut(s) 43, 52, 84, 393, 820
HpyF10VI GCNNNNNNNGC 8 cut(s) 19, 49, 95, 168, 504, 570, 833, 877
HpyF3I CTNAG 3 cut(s) 23, 279, 789
HpySE526I ACGT 3 cut(s) 360, 606, 699
Hsp92II CATG 2 cut(s) 715, 824
HspAI GCGC 8 cut(s) 9, 11, 123, 136, 157, 186, 573, 575
Kzo9I GATC 4 cut(s) 282, 600, 838, 933
LguI GCTCTTC 2 cut(s) 528, 647
LmnI GCTCC 4 cut(s) 345, 584, 802, 868
Lsp1109I GCAGC 4 cut(s) 101, 811, 889, 949
LweI GCATC 3 cut(s) 39, 201, 652
MaeII ACGT 3 cut(s) 360, 606, 699
MalI GATC 4 cut(s) 284, 602, 840, 935
MboI GATC 4 cut(s) 282, 600, 838, 933
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 5 cut(s) 342, 495, 523, 589, 873
MlyI GAGTC 3 cut(s) 641, 745, 974
MmeI TCCRAC 1 cut(s) 514
MroXI GAANNNNTTC 2 cut(s) 626, 950
MspI CCGG 7 cut(s) 303, 396, 471, 495, 555, 737, 866
MspR9I CCNGG 3 cut(s) 143, 209, 495
Mva1269I GAATGC 1 cut(s) 952
MvaI CCWGG 2 cut(s) 143, 209
MvnI CGCG 6 cut(s) 11, 123, 186, 564, 575, 704
MwoI GCNNNNNNNGC 8 cut(s) 19, 49, 95, 168, 504, 570, 833, 877
NciI CCSGG 1 cut(s) 495
NdeII GATC 4 cut(s) 282, 600, 838, 933
NlaIII CATG 2 cut(s) 715, 824
NlaIV GGNNCC 2 cut(s) 840, 870
NmeAIII GCCGAG 2 cut(s) 691, 938
NspI RCATGY 1 cut(s) 824
PaeI GCATGC 1 cut(s) 824
PaeR7I CTCGAG 2 cut(s) 431, 461
PauI GCGCGC 2 cut(s) 9, 573
PceI AGGCCT 1 cut(s) 110
PciSI GCTCTTC 2 cut(s) 528, 647
PcsI WCGNNNNNNNCGW 5 cut(s) 357, 438, 447, 495, 600
PctI GAATGC 1 cut(s) 952
PdmI GAANNNNTTC 2 cut(s) 626, 950
PfeI GAWTC 3 cut(s) 28, 636, 829
PflFI GACNNNGTC 2 cut(s) 272, 311
PleI GAGTC 3 cut(s) 641, 745, 974
PpsI GAGTC 3 cut(s) 641, 745, 974
Psp124BI GAGCTC 2 cut(s) 342, 589
Psp6I CCWGG 2 cut(s) 141, 207
PspGI CCWGG 2 cut(s) 141, 207
PspN4I GGNNCC 2 cut(s) 840, 870
PspPI GGNCC 1 cut(s) 510
PspXI VCTCGAGB 1 cut(s) 461
PstI CTGCAG 1 cut(s) 86
PsuI RGATCY 1 cut(s) 838
PsyI GACNNNGTC 2 cut(s) 272, 311
PteI GCGCGC 2 cut(s) 9, 573
RsaI GTAC 7 cut(s) 224, 239, 449, 683, 746, 767, 809
RsaNI GTAC 7 cut(s) 223, 238, 448, 682, 745, 766, 808
SacI GAGCTC 2 cut(s) 342, 589
SapI GCTCTTC 2 cut(s) 528, 647
Sau3AI GATC 4 cut(s) 282, 600, 838, 933
Sau96I GGNCC 1 cut(s) 510
SchI GAGTC 3 cut(s) 641, 745, 974
ScrFI CCNGG 3 cut(s) 143, 209, 495
SduI GDGCHC 5 cut(s) 342, 495, 523, 589, 873
SfaNI GCATC 3 cut(s) 39, 201, 652
SfcI CTRYAG 1 cut(s) 82
Sfr274I CTCGAG 2 cut(s) 431, 461
SgrAI CRCCGGYG 1 cut(s) 470
SlaI CTCGAG 2 cut(s) 431, 461
SmlI CTYRAG 3 cut(s) 431, 461, 966
SmoI CTYRAG 3 cut(s) 431, 461, 966
SphI GCATGC 1 cut(s) 824
SseBI AGGCCT 1 cut(s) 110
SstI GAGCTC 2 cut(s) 342, 589
StuI AGGCCT 1 cut(s) 110
StyD4I CCNGG 3 cut(s) 141, 207, 493
StyI CCWWGG 1 cut(s) 642
TaaI ACNGT 1 cut(s) 617
TaiI ACGT 3 cut(s) 363, 609, 702
TaqI TCGA 6 cut(s) 432, 462, 489, 603, 650, 936
TaqII GACCGA 1 cut(s) 191
TatI WGTACW 4 cut(s) 222, 681, 765, 807
TauI GCSGC 7 cut(s) 63, 162, 383, 417, 507, 613, 707
TfiI GAWTC 3 cut(s) 28, 636, 829
TscAI CASTG 1 cut(s) 622
TseI GCWGC 4 cut(s) 89, 824, 877, 962
TspDTI ATGAA 2 cut(s) 17, 91
TspGWI ACGGA 3 cut(s) 185, 475, 506
TspRI CASTG 1 cut(s) 622
Tth111I GACNNNGTC 2 cut(s) 272, 311
XceI RCATGY 1 cut(s) 824
XhoI CTCGAG 2 cut(s) 431, 461
XmnI GAANNNNTTC 2 cut(s) 626, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.