Rh4DG042700

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001819
Physical Location & Seq
Reverse (-)
6951795 .. 6953729
1935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG042700.1

Sequence Viewer

Length: 903 bp
ATGGCTTTCATCAAAGCTTCAAAACCCCGCGCCTACTTCAAGCGCTTCCAGGTCAAGTACAAGCGCCGCCGCGAAGGCAAGACCGACTACCGCGCCCGCATCCGCCTCATCAACCAAGACAAGAACAAGTACAACACCCCCAAGTACCGCTTCGTCGTCCGCTTCACCAACAAGGACATCGTCGCCCAGATCATCTCCGCCACCATCACCGGCGACCTCGTCCTCGCCTCCGCCTACGCCCACGAGCTCCCCCGCTACGGCCTCGATGTCGGCCTCACCAACTACGCCGCCTCCTACTGCACCGGCCTCCTCCTCGCCCGCCGTGTCCTCAAGAAGCTCGAGATGGACGCAGAATATGAAGGCAACGTCGAGGCCACCGGAGAGGACTACTCCGTCGAGCCCGGCGAGAGCCGCAGGCCGTTCCGGGCTCTGCTCGATGTCGGCCTGGTGAAGACCACTACCGGAAACCGGGTTTTCGGAGCGCTGAAGGGAGCTTTGGATGGGGGATTGGATGTGCCTCACAGTGAGAAGAGGTTCGCTGGGTTTTCGAAAGACTCGAAGCAGCTGGATGCCGAGGTTCACAGGAAGTATATCTATGGGGGCCATGTGGCGGCTTATATGGGGACTTTGATTGAGGATGAGCCGGAGAAGTACCAGACTCATTTCAGCGAGTACATCAAGAAGGGGATTGAGGCCGAGGGGATCGAGGAGCTGTACAAGAAGGTGCACGCCGCGATTCGGGCGGATCCTCTGGAGAAGAAGACTGCCAAGCCGGAGCCCAAGGCGCACAAGAGGTTCAACTTGAAGAAGCTGACTTATGAGGAGAGGAAGAACAAGTTGATCCAGAGGTTGAATGCCTTCAACTCGGCTGCTCAAGGTGATGAGGATTCGGATGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

300

Amino Acids

34.19

Weight (kDa)

9.41

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L5e PF17144 14 - 175 9.1e-81 Ribosomal large subunit proteins 60S L5, and 50S L18
Ribosomal_L18_c PF14204 235 - 288 1.9e-19 Ribosomal L18 C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
AccII CGCG 4 cut(s) 30, 72, 93, 734
AclWI GGATC 4 cut(s) 710, 740, 753, 835
AcuI CTGAAG 1 cut(s) 506
AfaI GTAC 6 cut(s) 59, 131, 146, 653, 674, 716
AfeI AGCGCT 2 cut(s) 44, 483
AfiI CCNNNNNNNGG 4 cut(s) 257, 468, 610, 738
AgsI TTSAA 6 cut(s) 21, 40, 799, 805, 853, 862
AjnI CCWGG 2 cut(s) 48, 444
AjuI GAANNNNNNNTTGG 4 cut(s) 134, 166, 479, 511
AluBI AGCT 7 cut(s) 17, 247, 337, 494, 565, 712, 811
AluI AGCT 7 cut(s) 17, 247, 337, 494, 565, 712, 811
Alw21I GWGCWC 2 cut(s) 249, 729
Alw44I GTGCAC 1 cut(s) 725
AlwI GGATC 4 cut(s) 710, 740, 753, 835
Ama87I CYCGRG 1 cut(s) 338
Aor51HI AGCGCT 2 cut(s) 44, 483
AoxI GGCC 8 cut(s) 259, 271, 304, 372, 416, 442, 601, 693
ApaLI GTGCAC 1 cut(s) 725
ApeKI GCWGC 2 cut(s) 562, 869
Asp700I GAANNNNTTC 2 cut(s) 533, 857
AspLEI GCGC 6 cut(s) 32, 45, 66, 95, 484, 787
AspS9I GGNCC 1 cut(s) 601
AsuC2I CCSGG 3 cut(s) 402, 425, 470
AsuHPI GGTGA 5 cut(s) 157, 199, 268, 460, 890
AsuII TTCGAA 1 cut(s) 548
AvaI CYCGRG 1 cut(s) 338
BaeGI GKGCMC 1 cut(s) 729
BamHI GGATCC 1 cut(s) 745
BanII GRGCYC 4 cut(s) 249, 402, 430, 780
BauI CACGAG 1 cut(s) 242
BbsI GAAGAC 2 cut(s) 458, 767
Bbv12I GWGCWC 2 cut(s) 249, 729
BbvI GCAGC 2 cut(s) 574, 856
BccI CCATC 3 cut(s) 212, 337, 494
BceAI ACGGC 3 cut(s) 274, 306, 403
BciT130I CCWGG 2 cut(s) 50, 446
BcnI CCSGG 3 cut(s) 402, 425, 470
BfoI RGCGCY 3 cut(s) 46, 67, 485
BglI GCCNNNNNGGC 1 cut(s) 75
BisI GCNGC 8 cut(s) 67, 70, 288, 412, 563, 612, 732, 870
BlsI GCNGC 8 cut(s) 68, 71, 289, 413, 564, 613, 733, 871
Bme1390I CCNGG 5 cut(s) 50, 402, 425, 446, 470
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 601
BmiI GGNNCC 3 cut(s) 602, 747, 777
BmrFI CCNGG 5 cut(s) 50, 402, 425, 446, 470
BmsI GCATC 2 cut(s) 108, 559
BpiI GAAGAC 2 cut(s) 458, 767
BplI GAGNNNNNCTC 2 cut(s) 374, 406
BpmI CTGGAG 1 cut(s) 773
Bpu14I TTCGAA 1 cut(s) 548
BpuEI CTTGAG 2 cut(s) 314, 858
BpuMI CCSGG 3 cut(s) 402, 425, 470
BsaJI CCNNGG 3 cut(s) 573, 696, 780
BsaWI WCCGGW 2 cut(s) 377, 461
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 4 cut(s) 257, 468, 610, 738
Bse118I RCCGGY 2 cut(s) 209, 302
BseBI CCWGG 2 cut(s) 50, 446
BseDI CCNNGG 3 cut(s) 573, 696, 780
BseGI GGATG 6 cut(s) 99, 505, 517, 574, 643, 898
BseLI CCNNNNNNNGG 4 cut(s) 257, 468, 610, 738
BseRI GAGGAG 4 cut(s) 299, 302, 722, 836
BseSI GKGCMC 1 cut(s) 729
BseXI GCAGC 2 cut(s) 574, 856
BseYI CCCAGC 1 cut(s) 539
BsgI GTGCAG 1 cut(s) 283
Bsh1236I CGCG 4 cut(s) 30, 72, 93, 734
BshFI GGCC 8 cut(s) 261, 273, 306, 374, 418, 444, 603, 695
BsiHKAI GWGCWC 2 cut(s) 249, 729
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 9 cut(s) 210, 303, 378, 402, 424, 462, 469, 644, 773
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 4 cut(s) 257, 468, 610, 738
BsmFI GGGAC 1 cut(s) 637
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 8 cut(s) 261, 273, 306, 374, 418, 444, 603, 695
BsoBI CYCGRG 1 cut(s) 338
Bsp119I TTCGAA 1 cut(s) 548
Bsp1286I GDGCHC 5 cut(s) 249, 402, 430, 729, 780
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 4 cut(s) 189, 702, 745, 840
BspANI GGCC 8 cut(s) 261, 273, 306, 374, 418, 444, 603, 695
BspFNI CGCG 4 cut(s) 30, 72, 93, 734
BspLI GGNNCC 3 cut(s) 602, 747, 777
BspPI GGATC 4 cut(s) 710, 740, 753, 835
BspT104I TTCGAA 1 cut(s) 548
BsrFI RCCGGY 2 cut(s) 209, 302
BsrGI TGTACA 1 cut(s) 714
BssAI RCCGGY 2 cut(s) 209, 302
BssECI CCNNGG 3 cut(s) 573, 696, 780
BssMI GATC 4 cut(s) 189, 702, 745, 840
BssSI CACGAG 1 cut(s) 242
BssT1I CCWWGG 1 cut(s) 780
Bst2BI CACGAG 1 cut(s) 242
Bst2UI CCWGG 2 cut(s) 50, 446
Bst4CI ACNGT 1 cut(s) 524
Bst6I CTCTTC 1 cut(s) 524
BstAUI TGTACA 1 cut(s) 714
BstBI TTCGAA 1 cut(s) 548
BstC8I GCNNGC 4 cut(s) 97, 319, 416, 729
BstF5I GGATG 6 cut(s) 99, 505, 517, 574, 643, 898
BstFNI CGCG 4 cut(s) 30, 72, 93, 734
BstH2I RGCGCY 3 cut(s) 46, 67, 485
BstHHI GCGC 6 cut(s) 32, 45, 66, 95, 484, 787
BstKTI GATC 4 cut(s) 192, 705, 748, 843
BstMBI GATC 4 cut(s) 189, 702, 745, 840
BstMWI GCNNNNNNNGC 4 cut(s) 75, 411, 740, 784
BstNI CCWGG 2 cut(s) 50, 446
BstSCI CCNGG 5 cut(s) 48, 400, 423, 444, 468
BstSLI GKGCMC 1 cut(s) 729
BstUI CGCG 4 cut(s) 30, 72, 93, 734
BstV1I GCAGC 2 cut(s) 574, 856
BstV2I GAAGAC 2 cut(s) 458, 767
BstX2I RGATCY 1 cut(s) 745
BstYI RGATCY 1 cut(s) 745
BsuRI GGCC 8 cut(s) 261, 273, 306, 374, 418, 444, 603, 695
BtsCI GGATG 6 cut(s) 99, 505, 517, 574, 643, 898
BtsIMutI CAGTG 1 cut(s) 529
Cac8I GCNNGC 4 cut(s) 97, 319, 416, 729
CfoI GCGC 6 cut(s) 32, 45, 66, 95, 484, 787
Cfr10I RCCGGY 2 cut(s) 209, 302
Cfr13I GGNCC 1 cut(s) 601
CseI GACGC 1 cut(s) 356
Csp6I GTAC 6 cut(s) 58, 130, 145, 652, 673, 715
CviAII CATG 1 cut(s) 605
CviQI GTAC 6 cut(s) 58, 130, 145, 652, 673, 715
DpnI GATC 4 cut(s) 191, 704, 747, 842
DpnII GATC 4 cut(s) 189, 702, 745, 840
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
Eam1104I CTCTTC 1 cut(s) 524
EarI CTCTTC 1 cut(s) 524
EciI GGCGGA 4 cut(s) 92, 187, 220, 758
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 780
Eco24I GRGCYC 4 cut(s) 249, 402, 430, 780
Eco47III AGCGCT 2 cut(s) 44, 483
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 506
Eco88I CYCGRG 1 cut(s) 338
EcoICRI GAGCTC 1 cut(s) 247
EcoRII CCWGG 2 cut(s) 48, 444
EcoT14I CCWWGG 1 cut(s) 780
EcoT38I GRGCYC 4 cut(s) 249, 402, 430, 780
ErhI CCWWGG 1 cut(s) 780
FaeI CATG 1 cut(s) 608
FaiI YATR 7 cut(s) 357, 591, 597, 606, 618, 620, 819
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 1 cut(s) 637
FatI CATG 1 cut(s) 604
FauI CCCGC 4 cut(s) 35, 104, 260, 326
Fnu4HI GCNGC 8 cut(s) 67, 70, 288, 412, 563, 612, 732, 870
FokI GGATG 5 cut(s) 86, 512, 524, 581, 650
FriOI GRGCYC 4 cut(s) 249, 402, 430, 780
Fsp4HI GCNGC 8 cut(s) 67, 70, 288, 412, 563, 612, 732, 870
GlaI GCGC 6 cut(s) 31, 44, 65, 94, 483, 786
GluI GCNGC 8 cut(s) 67, 70, 288, 412, 563, 612, 732, 870
GsaI CCCAGC 1 cut(s) 543
GsuI CTGGAG 1 cut(s) 773
HaeII RGCGCY 3 cut(s) 46, 67, 485
HaeIII GGCC 8 cut(s) 261, 273, 306, 374, 418, 444, 603, 695
HapII CCGG 9 cut(s) 210, 303, 378, 402, 424, 462, 469, 644, 773
HgaI GACGC 1 cut(s) 356
HhaI GCGC 6 cut(s) 32, 45, 66, 95, 484, 787
Hin1II CATG 1 cut(s) 608
Hin6I GCGC 6 cut(s) 30, 43, 64, 93, 482, 785
HinP1I GCGC 6 cut(s) 30, 43, 64, 93, 482, 785
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 4 cut(s) 554, 658, 736, 887
HpaII CCGG 9 cut(s) 210, 303, 378, 402, 424, 462, 469, 644, 773
HphI GGTGA 5 cut(s) 157, 199, 268, 460, 890
Hpy166II GTNNAC 2 cut(s) 580, 727
Hpy188I TCNGA 2 cut(s) 479, 892
Hpy188III TCNNGA 5 cut(s) 331, 340, 679, 752, 844
Hpy8I GTNNAC 2 cut(s) 580, 727
Hpy99I CGWCG 4 cut(s) 158, 185, 371, 398
HpyAV CCTTC 6 cut(s) 68, 353, 481, 676, 715, 868
HpyCH4III ACNGT 1 cut(s) 524
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 2 cut(s) 300, 727
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 411, 740, 784
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 1 cut(s) 608
HspAI GCGC 6 cut(s) 30, 43, 64, 93, 482, 785
Kzo9I GATC 4 cut(s) 189, 702, 745, 840
LmnI GCTCC 5 cut(s) 252, 479, 491, 709, 775
Lsp1109I GCAGC 2 cut(s) 574, 856
LweI GCATC 2 cut(s) 108, 559
MaeII ACGT 1 cut(s) 366
MalI GATC 4 cut(s) 191, 704, 747, 842
MboI GATC 4 cut(s) 189, 702, 745, 840
MboII GAAGA 6 cut(s) 463, 541, 769, 772, 817, 841
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 5 cut(s) 249, 402, 430, 729, 780
MlyI GAGTC 2 cut(s) 548, 652
MroXI GAANNNNTTC 2 cut(s) 533, 857
MspA1I CMGCKG 1 cut(s) 565
MspI CCGG 9 cut(s) 210, 303, 378, 402, 424, 462, 469, 644, 773
MspR9I CCNGG 5 cut(s) 50, 402, 425, 446, 470
Mva1269I GAATGC 1 cut(s) 859
MvaI CCWGG 2 cut(s) 50, 446
MvnI CGCG 4 cut(s) 30, 72, 93, 734
MwoI GCNNNNNNNGC 4 cut(s) 75, 411, 740, 784
NciI CCSGG 3 cut(s) 402, 425, 470
NdeII GATC 4 cut(s) 189, 702, 745, 840
NlaIII CATG 1 cut(s) 608
NlaIV GGNNCC 3 cut(s) 602, 747, 777
NmeAIII GCCGAG 3 cut(s) 598, 721, 845
NspV TTCGAA 1 cut(s) 548
PaeR7I CTCGAG 1 cut(s) 338
PcsI WCGNNNNNNNCGW 2 cut(s) 402, 554
PctI GAATGC 1 cut(s) 859
PdmI GAANNNNTTC 2 cut(s) 533, 857
PfeI GAWTC 2 cut(s) 736, 887
PflFI GACNNNGTC 2 cut(s) 179, 218
PkrI GCNGC 8 cut(s) 68, 71, 289, 413, 564, 613, 733, 871
PleI GAGTC 2 cut(s) 548, 652
PpsI GAGTC 2 cut(s) 548, 652
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 2 cut(s) 48, 444
PspFI CCCAGC 1 cut(s) 539
PspGI CCWGG 2 cut(s) 48, 444
PspN4I GGNNCC 3 cut(s) 602, 747, 777
PspPI GGNCC 1 cut(s) 601
PsuI RGATCY 1 cut(s) 745
PsyI GACNNNGTC 2 cut(s) 179, 218
PvuII CAGCTG 1 cut(s) 565
RsaI GTAC 6 cut(s) 59, 131, 146, 653, 674, 716
RsaNI GTAC 6 cut(s) 58, 130, 145, 652, 673, 715
SacI GAGCTC 1 cut(s) 249
SatI GCNGC 8 cut(s) 67, 70, 288, 412, 563, 612, 732, 870
Sau3AI GATC 4 cut(s) 189, 702, 745, 840
Sau96I GGNCC 1 cut(s) 601
SchI GAGTC 2 cut(s) 548, 652
ScrFI CCNGG 5 cut(s) 50, 402, 425, 446, 470
SduI GDGCHC 5 cut(s) 249, 402, 430, 729, 780
SfaNI GCATC 2 cut(s) 108, 559
Sfr274I CTCGAG 1 cut(s) 338
SfuI TTCGAA 1 cut(s) 548
SgrAI CRCCGGYG 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 338
SmlI CTYRAG 3 cut(s) 329, 338, 873
SmoI CTYRAG 3 cut(s) 329, 338, 873
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 5 cut(s) 48, 400, 423, 444, 468
StyI CCWWGG 1 cut(s) 780
TaaI ACNGT 1 cut(s) 524
TaiI ACGT 1 cut(s) 369
TaqI TCGA 8 cut(s) 264, 339, 369, 396, 435, 548, 557, 705
TaqII GACCGA 1 cut(s) 98
TatI WGTACW 4 cut(s) 57, 129, 672, 714
TauI GCSGC 6 cut(s) 69, 72, 290, 414, 614, 734
TfiI GAWTC 2 cut(s) 736, 887
TscAI CASTG 1 cut(s) 529
TseI GCWGC 2 cut(s) 562, 869
TspDTI ATGAA 1 cut(s) 372
TspGWI ACGGA 1 cut(s) 382
TspRI CASTG 1 cut(s) 529
Tth111I GACNNNGTC 2 cut(s) 179, 218
VneI GTGCAC 1 cut(s) 725
XhoI CTCGAG 1 cut(s) 338
XmnI GAANNNNTTC 2 cut(s) 533, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.