pycom02g00270

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001819
Physical Location & Seq
Reverse (-)
158515 .. 161109
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g00270.5

Sequence Viewer

Length: 909 bp
ATGCCGTTCGTCAAGTCTCAGAAGTCTAGGGCTTACTTCAAGAGGTTTCAAGTCAAGTATAAGAGAAGGAGAGAGGGGAAGACCGACTACCGTGCCAGGATTAGGCTCATTAACCAAGACAAGAACAAGTACAACACACCCAAGTATCGGTTTGTTGTGCGATTTACCAACAAGGATATTGTTGCCCAGATTGTATCTGCCAGCATTGCTGGCGATTTGGTTCTCGCTGCAGCCTATGCCCATGAGCTGCCACACTATGGACTTGAAGTTGGTCTGACAAACTATGCAGCAGCCTACTGCACTGGCCTACTCTTGGCTCGTCGTGTGCTGAAGAAGCTTGAAATGGATGACGAATATGAGGGGAATGTTGAGGCAACTGGTGAGGATTATTCTGTAGAGCCAGCTGAGAGCAGGAGGCCATTCCGTGCTCTTCTTGATGTTGGCCTGATCAGGACCACCACAGGAAATCGTGTATTCGGTGCCTTGAAGGGTGCTTTGGATGGGGGTTTGGATGTCCCTCACAGTGAAAAGAGGTTTGCCGGTTTCTCCAAGGATAGCAAGCAACTTGACGCTGAGGTTCATCGCAAGTACATCTATGGTGGCCATGTTGCTGCATACATGACAACTTTGGCGGATGATGAGCCGGAGAAATACCAGAGTCACTTCAGTGAATATATTAAGAAAGGTATTGAAGCGGATAACATCGAAGAGCTGTACAAGAAAGTTCATGCTGCCATCCGTGCTGATCCTACAATTAAGAAGACTGAGAAGCCAGCTCCTCAAGAGCACAAGAGGTACAACCTCAAGAAGCTTACCTTTGACGAGAGGAAGAAGAAGTTGGTTGAGAGGTTGAAGGCCTTCAATGAAGCTGGTAACGATGAAAACAGCGACGATGATGATGAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000027 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006810 GO:0006913 GO:0006996 GO:0007275 GO:0007389 GO:0008097 GO:0008150 GO:0008152 GO:0008283 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009955 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010467 GO:0015934 GO:0016020 GO:0016043 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022622 GO:0022625 GO:0022626 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042254 GO:0042255 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046907 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0065003 GO:0070013 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18_c Ribosomal_L5e

Protein Analysis

303

Amino Acids

34.65

Weight (kDa)

9.16

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 479
AccB7I CCANNNNNTGG 1 cut(s) 257
AciI CCGC 2 cut(s) 632, 695
AclWI GGATC 1 cut(s) 740
AcoI YGGCCR 1 cut(s) 601
AcuI CTGAAG 2 cut(s) 350, 649
AfaI GTAC 5 cut(s) 131, 590, 716, 797, 905
AfiI CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
AgsI TTSAA 8 cut(s) 40, 50, 266, 341, 487, 692, 853, 862
AjnI CCWGG 1 cut(s) 95
AjuI GAANNNNNNNTTGG 4 cut(s) 479, 511, 821, 853
AleI CACNNNNGTG 1 cut(s) 666
AluBI AGCT 7 cut(s) 247, 337, 404, 712, 776, 811, 869
AluI AGCT 7 cut(s) 247, 337, 404, 712, 776, 811, 869
Alw21I GWGCWC 2 cut(s) 430, 789
Alw26I GTCTC 1 cut(s) 21
AlwI GGATC 1 cut(s) 740
AoxI GGCC 5 cut(s) 304, 416, 442, 601, 855
ApeKI GCWGC 7 cut(s) 227, 230, 247, 287, 290, 611, 731
Asp700I GAANNNNTTC 1 cut(s) 857
AspS9I GGNCC 1 cut(s) 453
AsuHPI GGTGA 1 cut(s) 392
AvaII GGWCC 1 cut(s) 453
BaeI ACNNNNGTAYC 2 cut(s) 128, 161
BalI TGGCCA 1 cut(s) 603
BanI GGYRCC 1 cut(s) 479
BarI GAAGNNNNNNTAC 2 cut(s) 71, 103
BbsI GAAGAC 2 cut(s) 86, 767
Bbv12I GWGCWC 2 cut(s) 430, 789
BbvCI CCTCAGC 1 cut(s) 573
BbvI GCAGC 7 cut(s) 214, 234, 242, 299, 302, 598, 718
BccI CCATC 2 cut(s) 494, 743
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BciT130I CCWGG 1 cut(s) 97
BclI TGATCA 1 cut(s) 447
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 1 cut(s) 27
BfmI CTRYAG 2 cut(s) 228, 393
BisI GCNGC 7 cut(s) 228, 231, 248, 288, 291, 612, 732
BlsI GCNGC 7 cut(s) 229, 232, 249, 289, 292, 613, 733
BmcAI AGTACT 1 cut(s) 905
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 453
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 1 cut(s) 481
BmrFI CCNGG 1 cut(s) 97
BpiI GAAGAC 2 cut(s) 86, 767
Bpu10I CCTNAGC 1 cut(s) 573
BpuEI CTTGAG 2 cut(s) 765, 788
BsaJI CCNNGG 1 cut(s) 549
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
Bse118I RCCGGY 1 cut(s) 539
Bse1I ACTGG 2 cut(s) 307, 382
Bse3DI GCAATG 1 cut(s) 204
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 549
BseGI GGATG 5 cut(s) 352, 505, 517, 640, 735
BseLI CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 4 cut(s) 32, 396, 564, 756
BseNI ACTGG 2 cut(s) 307, 382
BseRI GAGGAG 1 cut(s) 768
BseXI GCAGC 7 cut(s) 214, 234, 242, 299, 302, 598, 718
BsgI GTGCAG 1 cut(s) 283
BshFI GGCC 5 cut(s) 306, 418, 444, 603, 857
BshNI GGYRCC 1 cut(s) 479
BsiHKAI GWGCWC 2 cut(s) 430, 789
BsiSI CCGG 2 cut(s) 540, 644
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 4 cut(s) 102, 147, 257, 313
BsmAI GTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 500
BsnI GGCC 5 cut(s) 306, 418, 444, 603, 857
Bsp1286I GDGCHC 2 cut(s) 430, 789
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 2 cut(s) 447, 745
BspACI CCGC 2 cut(s) 632, 695
BspANI GGCC 5 cut(s) 306, 418, 444, 603, 857
BspCNI CTCAG 4 cut(s) 31, 397, 565, 757
BspLI GGNNCC 1 cut(s) 481
BspMAI CTGCAG 1 cut(s) 232
BspPI GGATC 1 cut(s) 740
BspQI GCTCTTC 2 cut(s) 435, 702
BspT107I GGYRCC 1 cut(s) 479
BsrDI GCAATG 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 539
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 2 cut(s) 307, 382
BssAI RCCGGY 1 cut(s) 539
BssECI CCNNGG 1 cut(s) 549
BssMI GATC 2 cut(s) 447, 745
BssT1I CCWWGG 1 cut(s) 549
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 2 cut(s) 92, 524
Bst6I CTCTTC 2 cut(s) 435, 702
BstAPI GCANNNNNTGC 1 cut(s) 236
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 5 cut(s) 202, 211, 402, 560, 774
BstDEI CTNAG 4 cut(s) 18, 405, 573, 765
BstF5I GGATG 5 cut(s) 352, 505, 517, 640, 735
BstKTI GATC 2 cut(s) 450, 748
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 2 cut(s) 447, 745
BstMWI GCNNNNNNNGC 5 cut(s) 206, 210, 236, 334, 740
BstNI CCWGG 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 95
BstSFI CTRYAG 2 cut(s) 228, 393
BstV1I GCAGC 7 cut(s) 214, 234, 242, 299, 302, 598, 718
BstV2I GAAGAC 2 cut(s) 86, 767
BsuRI GGCC 5 cut(s) 306, 418, 444, 603, 857
BtgZI GCGATG 1 cut(s) 566
BtsCI GGATG 5 cut(s) 352, 505, 517, 640, 735
BtsIMutI CAGTG 3 cut(s) 300, 529, 673
Cac8I GCNNGC 5 cut(s) 202, 211, 402, 560, 774
Cfr10I RCCGGY 1 cut(s) 539
Cfr13I GGNCC 1 cut(s) 453
CseI GACGC 1 cut(s) 578
Csp6I GTAC 5 cut(s) 130, 589, 715, 796, 904
CviAII CATG 4 cut(s) 242, 605, 619, 728
CviQI GTAC 5 cut(s) 130, 589, 715, 796, 904
DdeI CTNAG 4 cut(s) 18, 405, 573, 765
DpnI GATC 2 cut(s) 449, 747
DpnII GATC 2 cut(s) 447, 745
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 2 cut(s) 435, 702
EarI CTCTTC 2 cut(s) 435, 702
EciI GGCGGA 1 cut(s) 647
Eco130I CCWWGG 1 cut(s) 549
Eco147I AGGCCT 1 cut(s) 857
Eco47I GGWCC 1 cut(s) 453
Eco57I CTGAAG 2 cut(s) 350, 649
EcoRII CCWGG 1 cut(s) 95
EcoT14I CCWWGG 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 549
FaeI CATG 4 cut(s) 245, 608, 622, 731
FalI AAGNNNNNCTT 2 cut(s) 800, 832
FaqI GGGAC 1 cut(s) 500
FatI CATG 4 cut(s) 241, 604, 618, 727
FbaI TGATCA 1 cut(s) 447
Fnu4HI GCNGC 7 cut(s) 228, 231, 248, 288, 291, 612, 732
FokI GGATG 5 cut(s) 359, 512, 524, 647, 722
Fsp4HI GCNGC 7 cut(s) 228, 231, 248, 288, 291, 612, 732
FspBI CTAG 1 cut(s) 27
GluI GCNGC 7 cut(s) 228, 231, 248, 288, 291, 612, 732
HaeIII GGCC 5 cut(s) 306, 418, 444, 603, 857
HapII CCGG 2 cut(s) 540, 644
HgaI GACGC 1 cut(s) 578
Hin1II CATG 4 cut(s) 245, 608, 622, 731
HindIII AAGCTT 2 cut(s) 335, 809
HinfI GANTC 1 cut(s) 658
HpaII CCGG 2 cut(s) 540, 644
HphI GGTGA 1 cut(s) 392
Hpy188I TCNGA 2 cut(s) 21, 276
Hpy188III TCNNGA 5 cut(s) 40, 434, 451, 782, 805
Hpy99I CGWCG 2 cut(s) 324, 893
HpyAV CCTTC 4 cut(s) 60, 481, 847, 868
HpyCH4III ACNGT 2 cut(s) 92, 524
HpyCH4V TGCA 4 cut(s) 230, 287, 300, 614
HpyF10VI GCNNNNNNNGC 5 cut(s) 206, 210, 236, 334, 740
HpyF3I CTNAG 4 cut(s) 18, 405, 573, 765
Hsp92II CATG 4 cut(s) 245, 608, 622, 731
Ksp22I TGATCA 1 cut(s) 447
Kzo9I GATC 2 cut(s) 447, 745
LguI GCTCTTC 2 cut(s) 435, 702
LmnI GCTCC 1 cut(s) 781
Lsp1109I GCAGC 7 cut(s) 214, 234, 242, 299, 302, 598, 718
MaeI CTAG 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 659, 872
MalI GATC 2 cut(s) 449, 747
MboI GATC 2 cut(s) 447, 745
MboII GAAGA 7 cut(s) 91, 343, 422, 719, 772, 841, 844
MhlI GDGCHC 2 cut(s) 430, 789
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 1 cut(s) 753
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 1 cut(s) 667
Mox20I TGGCCA 1 cut(s) 603
MroXI GAANNNNTTC 1 cut(s) 857
MscI TGGCCA 1 cut(s) 603
MseI TTAA 3 cut(s) 111, 678, 756
MslI CAYNNNNRTG 1 cut(s) 666
Msp20I TGGCCA 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 2 cut(s) 540, 644
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 5 cut(s) 206, 210, 236, 334, 740
NdeII GATC 2 cut(s) 447, 745
NlaIII CATG 4 cut(s) 245, 608, 622, 731
NlaIV GGNNCC 1 cut(s) 481
NmuCI GTSAC 1 cut(s) 659
OliI CACNNNNGTG 1 cut(s) 666
PceI AGGCCT 1 cut(s) 857
PciSI GCTCTTC 2 cut(s) 435, 702
PdmI GAANNNNTTC 1 cut(s) 857
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 7 cut(s) 229, 232, 249, 289, 292, 613, 733
PleI GAGTC 1 cut(s) 666
PpsI GAGTC 1 cut(s) 666
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 481
PspPI GGNCC 1 cut(s) 453
PstI CTGCAG 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 5 cut(s) 131, 590, 716, 797, 905
RsaNI GTAC 5 cut(s) 130, 589, 715, 796, 904
RseI CAYNNNNRTG 1 cut(s) 666
SapI GCTCTTC 2 cut(s) 435, 702
SaqAI TTAA 3 cut(s) 111, 678, 756
SatI GCNGC 7 cut(s) 228, 231, 248, 288, 291, 612, 732
Sau3AI GATC 2 cut(s) 447, 745
Sau96I GGNCC 1 cut(s) 453
ScaI AGTACT 1 cut(s) 905
SchI GAGTC 1 cut(s) 667
ScrFI CCNGG 1 cut(s) 97
SduI GDGCHC 2 cut(s) 430, 789
SfcI CTRYAG 2 cut(s) 228, 393
SinI GGWCC 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 2 cut(s) 780, 803
SmoI CTYRAG 2 cut(s) 780, 803
Sse9I AATT 1 cut(s) 753
SseBI AGGCCT 1 cut(s) 857
SsiI CCGC 2 cut(s) 632, 695
SspMI CTAG 1 cut(s) 27
StuI AGGCCT 1 cut(s) 857
StyD4I CCNGG 1 cut(s) 95
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 2 cut(s) 92, 524
TaqI TCGA 1 cut(s) 705
TaqII GACCGA 1 cut(s) 98
TasI AATT 1 cut(s) 753
TatI WGTACW 4 cut(s) 129, 588, 714, 903
Tru1I TTAA 3 cut(s) 111, 678, 756
Tru9I TTAA 3 cut(s) 111, 678, 756
TscAI CASTG 3 cut(s) 307, 529, 673
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 7 cut(s) 227, 230, 247, 287, 290, 611, 731
Tsp45I GTSAC 1 cut(s) 659
TspDTI ATGAA 4 cut(s) 569, 716, 879, 894
TspGWI ACGGA 2 cut(s) 413, 728
TspRI CASTG 3 cut(s) 307, 529, 673
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 857
XspI CTAG 1 cut(s) 27
ZrmI AGTACT 1 cut(s) 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.