Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Forward (+)

5286158 .. 5292270

Gene Structure 6113 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G08350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3126 bp

ATGCCGCGAAGCAGAGACGAAGACGATGAATTAGACGGTGACTACGAAGCATTAGATTTAGAGGAAGAAGAGGAAGAAGACGAAGAAGAAGAAGAAGAGAGAGGAAGAGGTGGCGGCGGTTCTAGACGGAAGAGAGGAAGATCGAATTTTATCGATGATTATGCTGAGGAAGATTCTCAGGAAGAAGATGATGACGATGAAGATTATGGTAGTAGCCGTGGAGGTAAAGGAGCTGCTAGTAAGAGAAAGAAGCCATCTGCTTCTATTTTCTTAGACCGAGAAGCTCATCAAGTTGATGATGAAGACGAAGAGGAAGAAGATGAAGCAGAAGATGATTTTATTGTGGATAATGGAACGGACCTTCCGGATGAGCGTGGTGATAGACGGTACGAACGGAGGTTTCTTCCTCGAGACGAAAATGATGAGGATGTGGAAGATCTCGAGAGAAGAATTCAAGAGCGGTTCTCCTCTAGGCATCATGAAGAATATGATGAAGAAGCTACAGAAGTTGAGCAACAAGCGCTTTTGCCATCAGTCCGTGATCCGAAATTGTGGATGGTGAAATGTGCGATTGGCCGTGAAAGGGAGGTTGCTGTTTGTCTTATGCAAAAATTTATAGATCGAGGAGCTGATTTGCAGATTAGATCTGTTGTTGCCCTTGACCATCTTAAGAATTTTATATATGTTGAAGCAGACAAGGAAGCTCATGTGAAAGAGGCAATCAAGGGCATGAGGAATATATATGCTAATCAGAAGATCTTACTTGTCCCGATAAGAGAAATGACAGATGTTCTTTCTGTTGAGAGCAAAGCCATTGATCTATCACGGGATACCTGGGTTAGAATGAAAATCGGGACGTATAAAGGTGATCTTGCAAAGGTAGTTGATGTTGATAATGTGCGTCAGAGGGTCACAGTCAAGTTAATCCCAAGAATTGATCTGCAGGCACTAGCCAGTAAGCTGGACGGAAGAGAAGTTTCAAAGAAAAAAGCCTTTGTTCCACCTCCACGTTTCATGAACATTGATGAAGCGAGGGAACTGCACATACGTGTTGAGCGTCGGCGTGATCACATGACTGGTGATTACTTTGAAAATATTGGTGGCATGCTTTTCAAAGATGGGTTTCATTACAAACAAGTATCATTGAAGTCCATCACTGTTCAGAATGTTACACCAACTTTTGACGAACTTGAAAAATTCAATAAACCAAGTGAAAATGGAGAGGGCGACTTTGGTGGTCTGTCAACATTATTTGCAAACAGAAAGAAAGGTCATTTCATGAAGGGTGATGCAGTTATTGTTATCAAGGGGGATCTGAAAAACTTGAAGGGTTGGGTTGAGAAAGTAGATGAAGAAAATGTCCTTATCAGATCAGAAGTGAAAGGTCTCCCTGATCCTCTTGCTGTAAATGAGAGAGAGCTTTGCAAGTACTTTGAACCTGGAAATCATGTGAAGGTTGTCTCTGGGACCCATGAAGGAGCAACAGGCATGGTTGTCAAAGTTGATCAACACGTGCTTATCATTCTGTCTGACACGACCAAAGAACATGTCCGAGTGTTTGCTGATCATGTTGTTGAGAGTTCAGAAGTGACAACTGGTGTTACCAAAATTGGGGACTATGAACTTCATGATCTTGTGCTTCTGGATAACCTGAGCTTTGGAGTAATTATACGACTAGAGAATGAAGCTTTTCAGGTTCTTAAAGGGGTTCCTGACAGACCAGAGGTTGCTCTTGTCAAACTCAGAGAAATCAAATGCAAACTTGAGAAGAAAATCAATGTTCAAGATCGCTACAAAAATGTCATTGCTGTGAAAGATGACGTCAGAGTGATCGAGGGTCCTAGCAAAGGTAAACAAGGTCCTGTAAAGCATATCTACAAAGGAGTCTTATTTATATATGATCGACATCACCTTGAGCATGCTGGATTTATCTGCGCTAAATGCACATCATGCATTGTTGTTGGTGGATCACGTTCTGGTGCTAATAGAAATGGTGGTGATTCGCTTTCAAGGTATGGCAATTTCAAGGCACCTGCCCCAGTACCCTCATCTCCAGGAAGATTTCAACGTGGCAGAGGCGGAGGATATAATAACTCTGGAGGGAGGCATGGGGGTGGAAGAGGACGGGGAGACGATTCTTTATTGGGTACTACTGTTAAAATCCGTCTAGGACCTTTCAAGGGGTATAGGGGACCTGTAGTAGAAGTGAAAGGAAATTCAGTGCGTGTAGAACTTGAGATGAAGATTGTGACAGTTGATCGGGGTGCAATATCAGATAATGTTGCGACAACACCGTTTAGAGATACATCTCGGTATAGTATGGGAAGCGAAACACCTATGCATCCTTCACGGACTCCACTTCATCCTTATATGACTCCAATGCGGGACTCTGGAGCTACACCAATCCATGATGGAATGAGGACACCCATGCGTGATAGAGCTTGGAATCCTTACACGCCTATGAGTCCACCTAGGGATAACTGGGAAGATGGAAATCCGGGATCTTGGGGAACGAGTCCTCAATATCAGCCGGGAAGTCCTCCTTCACGGGCATATGAAGCACCAACGCCTGGCTCAGGATGGGCTAGTACTCCTGGTGGTAGTTACTCGGATGCGGGGACACCTAGAGACCATGGTTCAGCTTATGCTAATGCCCCAAGTCCTTACCTGCCATCAACACCTGGGCAACCCATGACACCAAGCTCGGCTTCATACTTACCTGGTACTCCCGGAGGACAACCAATGACTCCTGGAACTGGCCTCGATGTCATGTCTCCTGTTATAGGTGGGGATGCTGAAGCATGGTTCATGCCCGATATTTTGGTTGACATACACAAGGCTGGAGAGGACACCGATGTGGGCGTCATCCGAGATGTTTCGGATGGAACGTGTAAAGTGTCTCTCGGGTCCAGCGGTGAAGGTGACACTATAATGGCCCTCCCAAGCGAACTGGAGATAATTCCTCCGAGGAAATCCGATCGTGTCAAAATAGTTGGCGGTCAGTATCGTGGGTCTACAGGTAAGCTGATCGGAATCGACGGGTCTGACGGTATCGTTAAGATAGATGATAATCTTGACGTCAAGATTCTGGACCTGGCCTTATTAGCCAAGTTTGTGCAACCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

1041

Amino Acids

115.4

Weight (kDa)

5.26

Isoelectric Point (pI)

38.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5_N	PF11942	90 - 177	1.6e-09	Spt5 transcription elongation factor, acidic N-terminal
Spt5-NGN	PF03439	183 - 265	3.3e-26	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	271 - 400	2.6e-89	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	428 - 477	3.1e-20	Transcription elongation factor SPT5, second KOW domain
KOWx_SPT5	PF23037	536 - 587	9.2e-17	Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5	PF23291	595 - 647	2.6e-22	Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5	PF23290	714 - 755	6.3e-12	Transcription elongation factor SPT5, fifth KOW domain
KOW6_SPT51-2	PF23038	936 - 985	1.2e-22	Transcription elongation factor SPT5 homolog 1/2, KOW6 domain
KOW7_SPT5	PF23287	988 - 1037	7.9e-22	Transcription elongation factor SPT5, seventh KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2041
AatII	GACGTC	2 cut(s)	1824, 3081
Acc36I	ACCTGC	2 cut(s)	2041, 2676
AccB1I	GGYRCC	1 cut(s)	2029
AccB7I	CCANNNNNTGG	1 cut(s)	2570
AccBSI	CCGCTC	1 cut(s)	460
AccI	GTMKAC	1 cut(s)	3014
AccII	CGCG	1 cut(s)	7
AccIII	TCCGGA	1 cut(s)	364
AciI	CCGC	9 cut(s)	5, 114, 117, 460, 2079, 2383, 2615, 2913, 2997
AclWI	GGATC	5 cut(s)	536, 1320, 1388, 1975, 2509
AcoI	YGGCCR	1 cut(s)	574
AcsI	RAATTY	6 cut(s)	145, 450, 611, 673, 1196, 2215
AcuI	CTGAAG	1 cut(s)	2817
AcvI	CACGTG	1 cut(s)	1513
AcyI	GRCGYC	3 cut(s)	1821, 2862, 3078
AfaI	GTAC	6 cut(s)	389, 1430, 2043, 2149, 2590, 2725
AfeI	AGCGCT	1 cut(s)	522
AfiI	CCNNNNNNNGG	8 cut(s)	583, 1611, 1847, 2180, 2570, 2576, 2756, 2783
AflII	CTTAAG	1 cut(s)	668
AflIII	ACRYGT	4 cut(s)	1048, 1510, 1546, 2888
AjnI	CCWGG	9 cut(s)	833, 1438, 2053, 2569, 2593, 2680, 2719, 2749, 3093
AjuI	GAANNNNNNNTTGG	2 cut(s)	2692, 2724
AleI	CACNNNNGTG	2 cut(s)	1047, 2855
AloI	GAACNNNNNNTCC	2 cut(s)	346, 378
Alw26I	GTCTC	8 cut(s)	9, 405, 1391, 1465, 2124, 2622, 2778, 2904
AlwI	GGATC	5 cut(s)	536, 1320, 1388, 1975, 2509
Ama87I	CYCGRG	3 cut(s)	408, 440, 2903
Aor13HI	TCCGGA	1 cut(s)	364
Aor51HI	AGCGCT	1 cut(s)	522
AoxI	GGCC	4 cut(s)	574, 2758, 2934, 3096
ApeKI	GCWGC	1 cut(s)	233
ApoI	RAATTY	6 cut(s)	145, 450, 611, 673, 1196, 2215
ArsI	GACNNNNNNTTYG	2 cut(s)	1533, 1565
Asp700I	GAANNNNTTC	1 cut(s)	1689
AspA2I	CCTAGG	1 cut(s)	2471
AspLEI	GCGC	2 cut(s)	523, 1937
AspS9I	GGNCC	9 cut(s)	358, 1467, 1838, 1859, 2171, 2192, 2907, 2935, 3091
AsuC2I	CCSGG	3 cut(s)	2499, 2532, 2730
AvaI	CYCGRG	3 cut(s)	408, 440, 2903
AvaII	GGWCC	8 cut(s)	358, 1467, 1838, 1859, 2171, 2192, 2907, 3091
AvrII	CCTAGG	1 cut(s)	2471
BaeI	ACNNNNGTAYC	4 cut(s)	822, 855, 2139, 2172
BanI	GGYRCC	1 cut(s)	2029
BbrPI	CACGTG	1 cut(s)	1513
BbsI	GAAGAC	3 cut(s)	27, 84, 309
BbvCI	CCTCAGC	1 cut(s)	165
BbvI	GCAGC	1 cut(s)	220
BceAI	ACGGC	2 cut(s)	201, 561
BcgI	CGANNNNNNTGC	4 cut(s)	143, 177, 1021, 1055
BciT130I	CCWGG	9 cut(s)	835, 1440, 2055, 2571, 2595, 2682, 2721, 2751, 3095
BciVI	GTATCC	1 cut(s)	823
BclI	TGATCA	3 cut(s)	1066, 1504, 1564
BcnI	CCSGG	3 cut(s)	2499, 2532, 2730
BcoDI	GTCTC	8 cut(s)	9, 405, 1391, 1465, 2124, 2622, 2778, 2904
BfmI	CTRYAG	4 cut(s)	501, 941, 2196, 3015
BfoI	RGCGCY	1 cut(s)	524
BfrI	CTTAAG	1 cut(s)	668
BfuAI	ACCTGC	2 cut(s)	2041, 2676
BfuI	GTATCC	1 cut(s)	823
BglII	AGATCT	3 cut(s)	436, 644, 756
BisI	GCNGC	3 cut(s)	5, 115, 234
BlnI	CCTAGG	1 cut(s)	2471
BlsI	GCNGC	3 cut(s)	6, 116, 235
BmcAI	AGTACT	2 cut(s)	1430, 2590
Bme18I	GGWCC	8 cut(s)	358, 1467, 1838, 1859, 2171, 2192, 2907, 3091
BmeT110I	CYCGRG	3 cut(s)	408, 440, 2903
BmgT120I	GGNCC	9 cut(s)	358, 1467, 1838, 1859, 2171, 2192, 2907, 2935, 3091
BmiI	GGNNCC	7 cut(s)	1468, 1469, 1710, 1839, 2031, 2193, 2908
BmrI	ACTGGG	2 cut(s)	2033, 2491
BmsI	GCATC	5 cut(s)	484, 1279, 2350, 2602, 2782
BmuI	ACTGGG	2 cut(s)	2033, 2491
BpiI	GAAGAC	3 cut(s)	27, 84, 309
BplI	GAGNNNNNCTC	2 cut(s)	449, 481
BpmI	CTGGAG	5 cut(s)	2037, 2118, 2412, 2862, 2972
Bpu10I	CCTNAGC	3 cut(s)	165, 1652, 2575
BpuEI	CTTGAG	3 cut(s)	1784, 1934, 2255
BpuMI	CCSGG	3 cut(s)	2499, 2532, 2730
Bsa29I	ATCGAT	1 cut(s)	153
BsaAI	YACGTR	2 cut(s)	1049, 1513
BsaBI	GATNNNNATC	4 cut(s)	1905, 2493, 3032, 3069
BsaHI	GRCGYC	3 cut(s)	1821, 2862, 3078
BsaI	GGTCTC	2 cut(s)	1391, 2622
BsaJI	CCNNGG	6 cut(s)	217, 834, 2471, 2632, 2681, 2966
BsaWI	WCCGGW	1 cut(s)	364
Bsc4I	CCNNNNNNNGG	8 cut(s)	583, 1611, 1847, 2180, 2570, 2576, 2756, 2783
Bse1I	ACTGG	7 cut(s)	954, 1081, 1600, 2039, 2486, 2761, 2955
Bse3DI	GCAATG	1 cut(s)	1803
Bse8I	GATNNNNATC	4 cut(s)	1905, 2493, 3032, 3069
BseAI	TCCGGA	1 cut(s)	364
BseBI	CCWGG	9 cut(s)	835, 1440, 2055, 2571, 2595, 2682, 2721, 2751, 3095
BseCI	ATCGAT	1 cut(s)	153
BseDI	CCNNGG	6 cut(s)	217, 834, 2471, 2632, 2681, 2966
BseJI	GATNNNNATC	4 cut(s)	1905, 2493, 3032, 3069
BseLI	CCNNNNNNNGG	8 cut(s)	583, 1611, 1847, 2180, 2570, 2576, 2756, 2783
BseMI	GCAATG	1 cut(s)	1803
BseMII	CTCAG	5 cut(s)	156, 191, 1643, 1756, 2589
BseNI	ACTGG	7 cut(s)	954, 1081, 1600, 2039, 2486, 2761, 2955
BseRI	GAGGAG	2 cut(s)	457, 639
BseXI	GCAGC	1 cut(s)	220
BsgI	GTGCAG	1 cut(s)	1025
Bsh1236I	CGCG	1 cut(s)	7
Bsh1285I	CGRYCG	1 cut(s)	2980
BshFI	GGCC	4 cut(s)	576, 2760, 2936, 3098
BshNI	GGYRCC	1 cut(s)	2029
BshVI	ATCGAT	1 cut(s)	153
BsiEI	CGRYCG	1 cut(s)	2980
BsiHKCI	CYCGRG	3 cut(s)	408, 440, 2903
BsiSI	CCGG	4 cut(s)	365, 2498, 2531, 2730
BslFI	GGGAC	7 cut(s)	752, 868, 1480, 1628, 2205, 2399, 2632
BslI	CCNNNNNNNGG	8 cut(s)	583, 1611, 1847, 2180, 2570, 2576, 2756, 2783
BsmAI	GTCTC	8 cut(s)	9, 405, 1391, 1465, 2124, 2622, 2778, 2904
BsmBI	CGTCTC	3 cut(s)	9, 405, 2124
BsmFI	GGGAC	7 cut(s)	752, 868, 1480, 1628, 2205, 2399, 2632
BsnI	GGCC	4 cut(s)	576, 2760, 2936, 3098
Bso31I	GGTCTC	2 cut(s)	1391, 2622
BsoBI	CYCGRG	3 cut(s)	408, 440, 2903
Bsp13I	TCCGGA	1 cut(s)	364
Bsp19I	CCATGG	1 cut(s)	2632
BspACI	CCGC	9 cut(s)	5, 114, 117, 460, 2079, 2383, 2615, 2913, 2997
BspANI	GGCC	4 cut(s)	576, 2760, 2936, 3098
BspCNI	CTCAG	5 cut(s)	157, 190, 1644, 1755, 2588
BspDI	ATCGAT	1 cut(s)	153
BspEI	TCCGGA	1 cut(s)	364
BspFNI	CGCG	1 cut(s)	7
BspHI	TCATGA	4 cut(s)	478, 1014, 1278, 1627
BspLI	GGNNCC	7 cut(s)	1468, 1469, 1710, 1839, 2031, 2193, 2908
BspMAI	CTGCAG	1 cut(s)	945
BspMI	ACCTGC	2 cut(s)	2041, 2676
BspPI	GGATC	5 cut(s)	536, 1320, 1388, 1975, 2509
BspT107I	GGYRCC	1 cut(s)	2029
BspTI	CTTAAG	1 cut(s)	668
BspTNI	GGTCTC	2 cut(s)	1391, 2622
BsrBI	CCGCTC	1 cut(s)	460
BsrDI	GCAATG	1 cut(s)	1803
BsrI	ACTGG	7 cut(s)	954, 1081, 1600, 2039, 2486, 2761, 2955
BssECI	CCNNGG	6 cut(s)	217, 834, 2471, 2632, 2681, 2966
BssNI	GRCGYC	3 cut(s)	1821, 2862, 3078
BssT1I	CCWWGG	2 cut(s)	2471, 2632
Bst2UI	CCWGG	9 cut(s)	835, 1440, 2055, 2571, 2595, 2682, 2721, 2751, 3095
Bst4CI	ACNGT	9 cut(s)	38, 387, 916, 1159, 2155, 2254, 2295, 3050, 3123
Bst6I	CTCTTC	7 cut(s)	63, 90, 100, 125, 303, 964, 2113
BstACI	GRCGYC	3 cut(s)	1821, 2862, 3078
BstAFI	CTTAAG	1 cut(s)	668
BstAPI	GCANNNNNTGC	1 cut(s)	1950
BstBAI	YACGTR	2 cut(s)	1049, 1513
BstC8I	GCNNGC	3 cut(s)	945, 1106, 1920
BstDEI	CTNAG	6 cut(s)	165, 177, 271, 1652, 1742, 2575
BstDSI	CCRYGG	2 cut(s)	217, 2632
BstENI	CCTNNNNNAGG	3 cut(s)	1845, 2574, 2781
BstFNI	CGCG	1 cut(s)	7
BstH2I	RGCGCY	1 cut(s)	524
BstHHI	GCGC	2 cut(s)	523, 1937
BstMAI	GTCTC	8 cut(s)	9, 405, 1391, 1465, 2124, 2622, 2778, 2904
BstMCI	CGRYCG	1 cut(s)	2980
BstMWI	GCNNNNNNNGC	5 cut(s)	520, 1941, 1950, 2558, 3104
BstNI	CCWGG	9 cut(s)	835, 1440, 2055, 2571, 2595, 2682, 2721, 2751, 3095
BstNSI	RCATGY	3 cut(s)	1108, 1550, 1922
BstSFI	CTRYAG	4 cut(s)	501, 941, 2196, 3015
BstUI	CGCG	1 cut(s)	7
BstV1I	GCAGC	1 cut(s)	220
BstV2I	GAAGAC	3 cut(s)	27, 84, 309
BstX2I	RGATCY	5 cut(s)	436, 644, 756, 1312, 2501
BstXI	CCANNNNNNTGG	1 cut(s)	961
BstYI	RGATCY	5 cut(s)	436, 644, 756, 1312, 2501
Bsu15I	ATCGAT	1 cut(s)	153
BsuI	GTATCC	1 cut(s)	823
BsuRI	GGCC	4 cut(s)	576, 2760, 2936, 3098
BsuTUI	ATCGAT	1 cut(s)	153
BtgI	CCRYGG	2 cut(s)	217, 2632
BtsIMutI	CAGTG	2 cut(s)	1155, 2226
BveI	ACCTGC	2 cut(s)	2041, 2676
Cac8I	GCNNGC	3 cut(s)	945, 1106, 1920
CciI	TCATGA	4 cut(s)	478, 1014, 1278, 1627
CfoI	GCGC	2 cut(s)	523, 1937
Cfr13I	GGNCC	9 cut(s)	358, 1467, 1838, 1859, 2171, 2192, 2907, 2935, 3091
ClaI	ATCGAT	1 cut(s)	153
CseI	GACGC	3 cut(s)	890, 1046, 2851
CsiI	ACCWGGT	1 cut(s)	2719
Csp6I	GTAC	6 cut(s)	388, 1429, 2042, 2148, 2589, 2724
CviQI	GTAC	6 cut(s)	388, 1429, 2042, 2148, 2589, 2724
DdeI	CTNAG	6 cut(s)	165, 177, 271, 1652, 1742, 2575
EaeI	YGGCCR	1 cut(s)	574
Eam1104I	CTCTTC	7 cut(s)	63, 90, 100, 125, 303, 964, 2113
EarI	CTCTTC	7 cut(s)	63, 90, 100, 125, 303, 964, 2113
EciI	GGCGGA	1 cut(s)	2094
Eco130I	CCWWGG	2 cut(s)	2471, 2632
Eco31I	GGTCTC	2 cut(s)	1391, 2622
Eco47I	GGWCC	8 cut(s)	358, 1467, 1838, 1859, 2171, 2192, 2907, 3091
Eco47III	AGCGCT	1 cut(s)	522
Eco57I	CTGAAG	1 cut(s)	2817
Eco72I	CACGTG	1 cut(s)	1513
Eco88I	CYCGRG	3 cut(s)	408, 440, 2903
EcoNI	CCTNNNNNAGG	3 cut(s)	1845, 2574, 2781
EcoO109I	RGGNCCY	5 cut(s)	1467, 1838, 1859, 2171, 2192
EcoRI	GAATTC	1 cut(s)	450
EcoRII	CCWGG	9 cut(s)	833, 1438, 2053, 2569, 2593, 2680, 2719, 2749, 3093
EcoT14I	CCWWGG	2 cut(s)	2471, 2632
EcoT22I	ATGCAT	2 cut(s)	1955, 2343
ErhI	CCWWGG	2 cut(s)	2471, 2632
Esp3I	CGTCTC	3 cut(s)	9, 405, 2124
FalI	AAGNNNNNCTT	6 cut(s)	855, 887, 2527, 2559, 2692, 2724
FaqI	GGGAC	7 cut(s)	752, 868, 1480, 1628, 2205, 2399, 2632
FauI	CCCGC	2 cut(s)	2376, 2608
FauNDI	CATATG	1 cut(s)	2554
FbaI	TGATCA	3 cut(s)	1066, 1504, 1564
FblI	GTMKAC	1 cut(s)	3014
Fnu4HI	GCNGC	3 cut(s)	5, 115, 234
Fsp4HI	GCNGC	3 cut(s)	5, 115, 234
GlaI	GCGC	2 cut(s)	522, 1936
GluI	GCNGC	3 cut(s)	5, 115, 234
GsuI	CTGGAG	5 cut(s)	2037, 2118, 2412, 2862, 2972
HaeII	RGCGCY	1 cut(s)	524
HaeIII	GGCC	4 cut(s)	576, 2760, 2936, 3098
HapII	CCGG	4 cut(s)	365, 2498, 2531, 2730
HgaI	GACGC	3 cut(s)	890, 1046, 2851
HhaI	GCGC	2 cut(s)	523, 1937
Hin1I	GRCGYC	3 cut(s)	1821, 2862, 3078
Hin6I	GCGC	2 cut(s)	521, 1935
HinP1I	GCGC	2 cut(s)	521, 1935
HincII	GTYRAC	2 cut(s)	1245, 2827
HindII	GTYRAC	2 cut(s)	1245, 2827
HindIII	AAGCTT	1 cut(s)	1686
HpaII	CCGG	4 cut(s)	365, 2498, 2531, 2730
Hpy166II	GTNNAC	5 cut(s)	1245, 1853, 2468, 2827, 3015
Hpy8I	GTNNAC	5 cut(s)	1245, 1853, 2468, 2827, 3015
Hpy99I	CGWCG	2 cut(s)	1062, 3041
HpyAV	CCTTC	8 cut(s)	371, 1276, 1321, 1447, 1469, 2355, 2553, 2912
HpyCH4III	ACNGT	9 cut(s)	38, 387, 916, 1159, 2155, 2254, 2295, 3050, 3123
HpyCH4IV	ACGT	9 cut(s)	857, 1009, 1048, 1512, 1821, 1972, 2068, 2888, 3078
HpyF10VI	GCNNNNNNNGC	5 cut(s)	520, 1941, 1950, 2558, 3104
HpyF3I	CTNAG	6 cut(s)	165, 177, 271, 1652, 1742, 2575
HpySE526I	ACGT	9 cut(s)	857, 1009, 1048, 1512, 1821, 1972, 2068, 2888, 3078
Hsp92I	GRCGYC	3 cut(s)	1821, 2862, 3078
HspAI	GCGC	2 cut(s)	521, 1935
KflI	GGGWCCC	1 cut(s)	1467
Kpn2I	TCCGGA	1 cut(s)	364
Ksp22I	TGATCA	3 cut(s)	1066, 1504, 1564
LmnI	GCTCC	4 cut(s)	230, 626, 1478, 2393
Lsp1109I	GCAGC	1 cut(s)	220
LweI	GCATC	5 cut(s)	484, 1279, 2350, 2602, 2782
MabI	ACCWGGT	1 cut(s)	2719
MaeII	ACGT	9 cut(s)	857, 1009, 1048, 1512, 1821, 1972, 2068, 2888, 3078
MaeIII	GTNAC	8 cut(s)	38, 910, 1168, 1588, 1600, 2248, 2603, 2921
MbiI	CCGCTC	1 cut(s)	460
MflI	RGATCY	5 cut(s)	436, 644, 756, 1312, 2501
MlyI	GAGTC	7 cut(s)	1893, 2347, 2368, 2381, 2473, 2524, 2740
Mph1103I	ATGCAT	2 cut(s)	1955, 2343
MroI	TCCGGA	1 cut(s)	364
MroXI	GAANNNNTTC	1 cut(s)	1689
MseI	TTAA	5 cut(s)	669, 923, 1701, 2157, 3057
MslI	CAYNNNNRTG	6 cut(s)	1047, 1808, 2112, 2459, 2855, 2930
MspA1I	CMGCKG	1 cut(s)	2913
MspCI	CTTAAG	1 cut(s)	668
MspI	CCGG	4 cut(s)	365, 2498, 2531, 2730
MvaI	CCWGG	9 cut(s)	835, 1440, 2055, 2571, 2595, 2682, 2721, 2751, 3095
MvnI	CGCG	1 cut(s)	7
MwoI	GCNNNNNNNGC	5 cut(s)	520, 1941, 1950, 2558, 3104
NciI	CCSGG	3 cut(s)	2499, 2532, 2730
NcoI	CCATGG	1 cut(s)	2632
NdeI	CATATG	1 cut(s)	2554
NlaIV	GGNNCC	7 cut(s)	1468, 1469, 1710, 1839, 2031, 2193, 2908
NmeAIII	GCCGAG	1 cut(s)	2684
NmuCI	GTSAC	5 cut(s)	38, 910, 1588, 2248, 2921
NsiI	ATGCAT	2 cut(s)	1955, 2343
NspI	RCATGY	3 cut(s)	1108, 1550, 1922
OliI	CACNNNNGTG	2 cut(s)	1047, 2855
PaeI	GCATGC	2 cut(s)	1108, 1922
PaeR7I	CTCGAG	2 cut(s)	408, 440
PagI	TCATGA	4 cut(s)	478, 1014, 1278, 1627
PaqCI	CACCTGC	1 cut(s)	2041
PciI	ACATGT	1 cut(s)	1546
PcsI	WCGNNNNNNNCGW	3 cut(s)	42, 1054, 2885
PdmI	GAANNNNTTC	1 cut(s)	1689
PfeI	GAWTC	6 cut(s)	173, 2000, 2135, 2446, 3033, 3085
PflMI	CCANNNNNTGG	1 cut(s)	2570
PfoI	TCCNGGA	4 cut(s)	2053, 2497, 2728, 2749
PkrI	GCNGC	3 cut(s)	6, 116, 235
Ple19I	CGATCG	1 cut(s)	2980
PleI	GAGTC	7 cut(s)	1892, 2347, 2368, 2381, 2472, 2523, 2740
PmaCI	CACGTG	1 cut(s)	1513
PmlI	CACGTG	1 cut(s)	1513
PpsI	GAGTC	7 cut(s)	1892, 2347, 2368, 2381, 2472, 2523, 2740
Ppu21I	YACGTR	2 cut(s)	1049, 1513
PpuMI	RGGWCCY	5 cut(s)	1467, 1838, 1859, 2171, 2192
PscI	ACATGT	1 cut(s)	1546
Psp5II	RGGWCCY	5 cut(s)	1467, 1838, 1859, 2171, 2192
Psp6I	CCWGG	9 cut(s)	833, 1438, 2053, 2569, 2593, 2680, 2719, 2749, 3093
PspCI	CACGTG	1 cut(s)	1513
PspGI	CCWGG	9 cut(s)	833, 1438, 2053, 2569, 2593, 2680, 2719, 2749, 3093
PspN4I	GGNNCC	7 cut(s)	1468, 1469, 1710, 1839, 2031, 2193, 2908
PspPI	GGNCC	9 cut(s)	358, 1467, 1838, 1859, 2171, 2192, 2907, 2935, 3091
PspPPI	RGGWCCY	5 cut(s)	1467, 1838, 1859, 2171, 2192
PsrI	GAACNNNNNNTAC	2 cut(s)	1029, 1061
PstI	CTGCAG	1 cut(s)	945
PsuI	RGATCY	5 cut(s)	436, 644, 756, 1312, 2501
PvuI	CGATCG	1 cut(s)	2980
RsaI	GTAC	6 cut(s)	389, 1430, 2043, 2149, 2590, 2725
RsaNI	GTAC	6 cut(s)	388, 1429, 2042, 2148, 2589, 2724
RseI	CAYNNNNRTG	6 cut(s)	1047, 1808, 2112, 2459, 2855, 2930
SaqAI	TTAA	5 cut(s)	669, 923, 1701, 2157, 3057
SatI	GCNGC	3 cut(s)	5, 115, 234
Sau96I	GGNCC	9 cut(s)	358, 1467, 1838, 1859, 2171, 2192, 2907, 2935, 3091
ScaI	AGTACT	2 cut(s)	1430, 2590
SchI	GAGTC	7 cut(s)	1893, 2347, 2368, 2381, 2473, 2524, 2740
SexAI	ACCWGGT	1 cut(s)	2719
SfaNI	GCATC	5 cut(s)	484, 1279, 2350, 2602, 2782
SfcI	CTRYAG	4 cut(s)	501, 941, 2196, 3015
Sfr274I	CTCGAG	2 cut(s)	408, 440
SinI	GGWCC	8 cut(s)	358, 1467, 1838, 1859, 2171, 2192, 2907, 3091
SlaI	CTCGAG	2 cut(s)	408, 440
SmiMI	CAYNNNNRTG	6 cut(s)	1047, 1808, 2112, 2459, 2855, 2930
SmlI	CTYRAG	6 cut(s)	408, 440, 668, 1763, 1913, 2234
SmoI	CTYRAG	6 cut(s)	408, 440, 668, 1763, 1913, 2234
SphI	GCATGC	2 cut(s)	1108, 1922
SsiI	CCGC	9 cut(s)	5, 114, 117, 460, 2079, 2383, 2615, 2913, 2997
SspI	AATATT	1 cut(s)	1096
StyI	CCWWGG	2 cut(s)	2471, 2632
TaaI	ACNGT	9 cut(s)	38, 387, 916, 1159, 2155, 2254, 2295, 3050, 3123
TaiI	ACGT	9 cut(s)	860, 1012, 1051, 1515, 1824, 1975, 2071, 2891, 3081
TaqI	TCGA	9 cut(s)	143, 153, 409, 441, 622, 1833, 1903, 2763, 3036
TaqII	GACCGA	1 cut(s)	291
TatI	WGTACW	2 cut(s)	1428, 2588
TauI	GCSGC	2 cut(s)	7, 117
TfiI	GAWTC	6 cut(s)	173, 2000, 2135, 2446, 3033, 3085
Tru1I	TTAA	5 cut(s)	669, 923, 1701, 2157, 3057
Tru9I	TTAA	5 cut(s)	669, 923, 1701, 2157, 3057
TscAI	CASTG	2 cut(s)	1162, 2226
TseFI	GTSAC	5 cut(s)	38, 910, 1588, 2248, 2921
TseI	GCWGC	1 cut(s)	233
Tsp45I	GTSAC	5 cut(s)	38, 910, 1588, 2248, 2921
TspGWI	ACGGA	7 cut(s)	142, 371, 409, 527, 981, 2153, 2365
TspRI	CASTG	2 cut(s)	1162, 2226
Van91I	CCANNNNNTGG	1 cut(s)	2570
Vha464I	CTTAAG	1 cut(s)	668
VpaK11BI	GGWCC	8 cut(s)	358, 1467, 1838, 1859, 2171, 2192, 2907, 3091
XagI	CCTNNNNNAGG	3 cut(s)	1845, 2574, 2781
XapI	RAATTY	6 cut(s)	145, 450, 611, 673, 1196, 2215
XbaI	TCTAGA	1 cut(s)	122
XceI	RCATGY	3 cut(s)	1108, 1550, 1922
XhoI	CTCGAG	2 cut(s)	408, 440
XmaJI	CCTAGG	1 cut(s)	2471
XmiI	GTMKAC	1 cut(s)	3014
XmnI	GAANNNNTTC	1 cut(s)	1689
ZraI	GACGTC	2 cut(s)	1822, 3079
ZrmI	AGTACT	2 cut(s)	1430, 2590
Zsp2I	ATGCAT	2 cut(s)	1955, 2343

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.