Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Reverse (-)

49414026 .. 49416623

Gene Structure 2598 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG274100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGCAGCCAGAAGAAGAGGACGAAGAGCTCTACGAATACGAACCGGTTCGGCTTGGCTCGAATCGATTCGTAATCGTGGTCCTCCTCAATCTCCTTCTTCTCGCGGTTTTTCTCATTCTTATCCTTGGATTGGGCTTGGCGGCGTCATGCTTGGTAACCCTAGCCATGCCGAATCGGAGAGAGGATTATAAAGAAGAAGAGGAGAGCGAGATGCGGGAGGAGCGAAGGGCCAATTCGAAGCGGGGGAGATCGAATTCTGACGAGATTGAGATCCAGAAGCGGAGAGGGCGAGGGTCGAATTCGAAAAAGAGGAAATTGAAATCGGTTGCGGAGGAGGAGGAGGAGGAAGAAGGCGGTGGTGGAAGGTCTTTTAAGTCGATGTTTGTGGATGATGAGGCTATAGAGGATGAAGATGAGGATGAGGAGGAAGACGATGATGGTGAATTGGATGGGTTTATTGTTAATGAACCGGAGTTTGATAACGAACCTGATGAAGTTGATCATGATGGCGGCGGTGGGAGAAGGATGGGGTTTGTGTTGCCGGAGGAAGGAGAGGAGGAGGATATTGATGAGTTTGAAAGAAGGGTTCAAGACAGATTTAGGGATGTTCATGTGGAAGAATATGAGGATGATACGGAGGTTGGACAACAAGCTCTCATACCCGGTGTTAAGGACCCGAAATTGTGGTTGGTGAAGTGTGCCATTGGTAAAGAGAGAGGGGTAGCGGCTTGTCTAATGCAGAAGTGTATTGATAACCCTGGAGAATTGCAGATCATGTCTGCCATTGCTTTGGATCACTTGAAAAGCTTCATATATGTTGAAGCTTACAAAGAAGCCCATGTGAGGGAAGCTTGCAAAGGTCTGAGGTATGTAATTCTGCAGAAAATAATGCTTGTTCCTATTAAGGAGATGATTGATGTGCTATCATGTGAAAGCAAGAGCGTTGTAAATGATAAATGGGTTAGGATTAAGACGGGAGTATATAAAGGGGACCTTGCCAAGATTGTGGGGCATGTCCCGGGGAGACAGCAAGTTAGAGTAAAGTTAATTCCAAGGGTTGATTTACAAGCTCTTGCAAAAGGTGTAGAATCATCGAAGAAGAAGGAATCTGGTGCACGTCCTGATCGTGTTCCTGCACTTCTTAGGAACATGAACCTAGTTAGACAGCTGGGTGTACGAGTACAGTCCATGCGAGATCCATTGGCACGTGGTTATATTGAGAAGATTGATGGCTTATCTGGGATGATCTTTAAAGATGGTTTCCATCTTAAAGTTGTTTCTGATAAAAGTATAAAAGAGGCAAAACCCTCCTTGCAAGAACTTGAAGAATTCCAGGAACTTGATGGTGATGGAGATCATACAATTCCAAATAGAAAGAAAGGCGAAAGCTTTATGAAGGGCGATGCAGTTATTGTTACGGAGGGAGATCTGAGAAACTTGGTGGGACAGGTTGAGAAAGTTGAGTTAGGGACTGTTCATATCAAACCGGAAATGGATGGCCTCCCGGAAACTGTTGCCATCAATCAAACAGCACTTCAGAAATGCTTTAAAGAAGGGCAGCATGTGAAGGTTGTTAGTGGTAGTCAACAAGGAGCAACTGGCATGGTTGTTAAGGTTGATCAGCAGAAGCAGTTGATCACCATTTTATCTGATGCAACCAATGAAGCTATCTGTGTGTTCTCTGATCATGCTGTGGAGAGTCCTGAAGTGAATAATGGTGATACAATGGGCGACTCCCACTGCTTAAGGTATCACAAAATCCCTAGTCAAAGTCCGAGGTTTTTTGGAGGTGCAAGAAGGCATGATGGGCAGGATGGTTTTGGTAAAGGGAGCCGAGTAAAGTTCCGTCTAGGTCCCTATAAAGGCTGCAGGGGGCGTGTTGTGGAGGTTAAAGGATCAAATGTAAGGGTTGAACTAGAGTCCCAGATGAAAACTGTTGTTGTTGATCGTCTGCATTGTATTTCAGAATCAGGTAATGTTGCTGCTGCAGTTACAACACCATATCACTCTGGTTGTGAGACCCCAATGCCTTACATGAGGACTCCTATGAGAGATCCTGGAGCAACGTTAATGCCTTACATGACAACTCCCTTGAGAGATCCTGGAGCATCCCCAATGCGTTATATGACGACTCCTTACATGATGACTCCCAGAAGAGATCCCCCCCGAGCAACACCAATACATGGTGGCATGAGAACTCCACCTATGACTCCCATGAGAGATCCCCGAGCAACACCGATACATGGTGGCATGAGGACCCCTCCTATATATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

757

Amino Acids

84.77

Weight (kDa)

5.45

Isoelectric Point (pI)

48.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5-NGN	PF03439	227 - 308	2.1e-23	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	316 - 444	3.7e-27	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	466 - 515	1.6e-16	Transcription elongation factor SPT5, second KOW domain
KOW5_SPT5	PF23290	610 - 650	2.5e-12	Transcription elongation factor SPT5, fifth KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	189
AccB7I	CCANNNNNTGG	1 cut(s)	2183
AccII	CGCG	1 cut(s)	104
AclI	AACGTT	1 cut(s)	2066
AclWI	GGATC	8 cut(s)	265, 801, 1190, 1903, 2048, 2093, 2153, 2216
AcsI	RAATTY	3 cut(s)	253, 298, 1328
AcuI	CTGAAG	2 cut(s)	1520, 1725
AcvI	CACGTG	1 cut(s)	1208
AcyI	GRCGYC	1 cut(s)	143
AfaI	GTAC	2 cut(s)	1176, 1182
AfiI	CCNNNNNNNGG	7 cut(s)	130, 548, 843, 844, 1862, 2183, 2243
AflII	CTTAAG	1 cut(s)	1744
AgeI	ACCGGT	1 cut(s)	43
AgsI	TTSAA	7 cut(s)	319, 578, 590, 802, 821, 1325, 1913
AjiI	CACGTC	1 cut(s)	1118
AjnI	CCWGG	4 cut(s)	757, 1332, 2056, 2101
AjuI	GAANNNNNNNTTGG	2 cut(s)	671, 703
AluBI	AGCT	9 cut(s)	28, 653, 807, 824, 851, 1070, 1168, 1389, 1667
AluI	AGCT	9 cut(s)	28, 653, 807, 824, 851, 1070, 1168, 1389, 1667
Alw21I	GWGCWC	2 cut(s)	30, 1117
Alw26I	GTCTC	2 cut(s)	1018, 2012
Alw44I	GTGCAC	1 cut(s)	1113
AlwI	GGATC	8 cut(s)	265, 801, 1190, 1903, 2048, 2093, 2153, 2216
Ama87I	CYCGRG	3 cut(s)	1018, 2166, 2226
AoxI	GGCC	2 cut(s)	228, 1498
ApaLI	GTGCAC	1 cut(s)	1113
ApeKI	GCWGC	5 cut(s)	4, 1558, 1866, 1982, 1985
ApoI	RAATTY	3 cut(s)	253, 298, 1328
AsiGI	ACCGGT	1 cut(s)	43
Asp700I	GAANNNNTTC	3 cut(s)	45, 65, 806
AspS9I	GGNCC	6 cut(s)	79, 228, 673, 991, 1853, 2256
AsuC2I	CCSGG	4 cut(s)	663, 1019, 1020, 1505
AsuHPI	GGTGA	5 cut(s)	452, 703, 1358, 1630, 1730
AsuII	TTCGAA	2 cut(s)	236, 302
AvaI	CYCGRG	3 cut(s)	1018, 2166, 2226
AvaII	GGWCC	5 cut(s)	79, 673, 991, 1853, 2256
BaeGI	GKGCMC	1 cut(s)	1117
BaeI	ACNNNNGTAYC	2 cut(s)	624, 657
BanII	GRGCYC	1 cut(s)	30
BbrPI	CACGTG	1 cut(s)	1208
BbsI	GAAGAC	1 cut(s)	435
Bbv12I	GWGCWC	2 cut(s)	30, 1117
BbvI	GCAGC	5 cut(s)	16, 1570, 1853, 1969, 1972
BciT130I	CCWGG	4 cut(s)	759, 1334, 2058, 2103
BclI	TGATCA	4 cut(s)	499, 1618, 1635, 1684
BcnI	CCSGG	4 cut(s)	663, 1019, 1020, 1505
BcoDI	GTCTC	2 cut(s)	1018, 2012
BfaI	CTAG	5 cut(s)	161, 1157, 1764, 1850, 1916
BfmI	CTRYAG	4 cut(s)	399, 878, 1867, 1986
BfrI	CTTAAG	1 cut(s)	1744
BglII	AGATCT	1 cut(s)	1426
BisI	GCNGC	8 cut(s)	5, 141, 511, 726, 1559, 1867, 1983, 1986
BlsI	GCNGC	8 cut(s)	6, 142, 512, 727, 1560, 1868, 1984, 1987
Bme1390I	CCNGG	8 cut(s)	663, 759, 1019, 1020, 1334, 1505, 2058, 2103
Bme18I	GGWCC	5 cut(s)	79, 673, 991, 1853, 2256
BmeT110I	CYCGRG	3 cut(s)	1018, 2166, 2226
BmgBI	CACGTC	1 cut(s)	1118
BmgT120I	GGNCC	6 cut(s)	79, 228, 673, 991, 1853, 2256
BmiI	GGNNCC	5 cut(s)	675, 992, 1832, 1855, 2258
BmrFI	CCNGG	8 cut(s)	663, 759, 1019, 1020, 1334, 1505, 2058, 2103
BmsI	GCATC	4 cut(s)	201, 1393, 1642, 2117
BpiI	GAAGAC	1 cut(s)	435
BplI	GAGNNNNNCTC	1 cut(s)	2245
BpmI	CTGGAG	3 cut(s)	780, 2079, 2124
Bpu14I	TTCGAA	2 cut(s)	236, 302
BpuEI	CTTGAG	1 cut(s)	2113
BpuMI	CCSGG	4 cut(s)	663, 1019, 1020, 1505
Bsa29I	ATCGAT	1 cut(s)	64
BsaAI	YACGTR	1 cut(s)	1208
BsaBI	GATNNNNATC	2 cut(s)	269, 1353
BsaHI	GRCGYC	1 cut(s)	143
BsaI	GGTCTC	1 cut(s)	2012
BsaJI	CCNNGG	6 cut(s)	124, 757, 1018, 1019, 1052, 1775
BsaWI	WCCGGW	3 cut(s)	43, 469, 1486
Bsc4I	CCNNNNNNNGG	7 cut(s)	130, 548, 843, 844, 1862, 2183, 2243
Bse118I	RCCGGY	1 cut(s)	43
Bse1I	ACTGG	1 cut(s)	1603
Bse3DI	GCAATG	1 cut(s)	783
Bse8I	GATNNNNATC	2 cut(s)	269, 1353
BseBI	CCWGG	4 cut(s)	759, 1334, 2058, 2103
BseCI	ATCGAT	1 cut(s)	64
BseDI	CCNNGG	6 cut(s)	124, 757, 1018, 1019, 1052, 1775
BseJI	GATNNNNATC	2 cut(s)	269, 1353
BseLI	CCNNNNNNNGG	7 cut(s)	130, 548, 843, 844, 1862, 2183, 2243
BseMI	GCAATG	1 cut(s)	783
BseMII	CTCAG	2 cut(s)	854, 1421
BseNI	ACTGG	1 cut(s)	1603
BseSI	GKGCMC	1 cut(s)	1117
BseXI	GCAGC	5 cut(s)	16, 1570, 1853, 1969, 1972
BseYI	CCCAGC	1 cut(s)	1168
BsgI	GTGCAG	1 cut(s)	1119
Bsh1236I	CGCG	1 cut(s)	104
BshFI	GGCC	2 cut(s)	230, 1500
BshTI	ACCGGT	1 cut(s)	43
BshVI	ATCGAT	1 cut(s)	64
BsiHKAI	GWGCWC	2 cut(s)	30, 1117
BsiHKCI	CYCGRG	3 cut(s)	1018, 2166, 2226
BsiSI	CCGG	7 cut(s)	44, 470, 542, 663, 1019, 1487, 1505
BslFI	GGGAC	6 cut(s)	1001, 1004, 1458, 1483, 1839, 1906
BslI	CCNNNNNNNGG	7 cut(s)	130, 548, 843, 844, 1862, 2183, 2243
BsmAI	GTCTC	2 cut(s)	1018, 2012
BsmFI	GGGAC	6 cut(s)	1001, 1004, 1458, 1483, 1839, 1906
BsnI	GGCC	2 cut(s)	230, 1500
Bso31I	GGTCTC	1 cut(s)	2012
BsoBI	CYCGRG	3 cut(s)	1018, 2166, 2226
Bsp119I	TTCGAA	2 cut(s)	236, 302
Bsp1286I	GDGCHC	2 cut(s)	30, 1117
BspANI	GGCC	2 cut(s)	230, 1500
BspCNI	CTCAG	2 cut(s)	855, 1422
BspDI	ATCGAT	1 cut(s)	64
BspFNI	CGCG	1 cut(s)	104
BspHI	TCATGA	1 cut(s)	502
BspLI	GGNNCC	5 cut(s)	675, 992, 1832, 1855, 2258
BspMAI	CTGCAG	3 cut(s)	882, 1871, 1990
BspPI	GGATC	8 cut(s)	265, 801, 1190, 1903, 2048, 2093, 2153, 2216
BspQI	GCTCTTC	1 cut(s)	18
BspT104I	TTCGAA	2 cut(s)	236, 302
BspTI	CTTAAG	1 cut(s)	1744
BspTNI	GGTCTC	1 cut(s)	2012
BsrDI	GCAATG	1 cut(s)	783
BsrFI	RCCGGY	1 cut(s)	43
BsrI	ACTGG	1 cut(s)	1603
BssAI	RCCGGY	1 cut(s)	43
BssECI	CCNNGG	6 cut(s)	124, 757, 1018, 1019, 1052, 1775
BssNI	GRCGYC	1 cut(s)	143
BssT1I	CCWWGG	2 cut(s)	124, 1052
Bst2UI	CCWGG	4 cut(s)	759, 1334, 2058, 2103
Bst4CI	ACNGT	4 cut(s)	1185, 1474, 1513, 1936
Bst6I	CTCTTC	4 cut(s)	9, 18, 192, 2149
BstACI	GRCGYC	1 cut(s)	143
BstAFI	CTTAAG	1 cut(s)	1744
BstBAI	YACGTR	1 cut(s)	1208
BstBI	TTCGAA	2 cut(s)	236, 302
BstC8I	GCNNGC	1 cut(s)	853
BstDEI	CTNAG	3 cut(s)	863, 1142, 1430
BstEII	GGTNACC	1 cut(s)	154
BstFNI	CGCG	1 cut(s)	104
BstMAI	GTCTC	2 cut(s)	1018, 2012
BstMWI	GCNNNNNNNGC	3 cut(s)	220, 286, 1807
BstNI	CCWGG	4 cut(s)	759, 1334, 2058, 2103
BstNSI	RCATGY	2 cut(s)	1016, 1565
BstPI	GGTNACC	1 cut(s)	154
BstSCI	CCNGG	8 cut(s)	661, 757, 1017, 1018, 1332, 1503, 2056, 2101
BstSFI	CTRYAG	4 cut(s)	399, 878, 1867, 1986
BstSLI	GKGCMC	1 cut(s)	1117
BstUI	CGCG	1 cut(s)	104
BstV1I	GCAGC	5 cut(s)	16, 1570, 1853, 1969, 1972
BstV2I	GAAGAC	1 cut(s)	435
BstX2I	RGATCY	7 cut(s)	270, 1195, 1426, 2053, 2098, 2158, 2221
BstXI	CCANNNNNNTGG	2 cut(s)	790, 1006
BstYI	RGATCY	7 cut(s)	270, 1195, 1426, 2053, 2098, 2158, 2221
Bsu15I	ATCGAT	1 cut(s)	64
BsuRI	GGCC	2 cut(s)	230, 1500
BsuTUI	ATCGAT	1 cut(s)	64
BtgZI	GCGATG	1 cut(s)	1416
BtrI	CACGTC	1 cut(s)	1118
BtsI	GCAGTG	1 cut(s)	1738
BtsIMutI	CAGTG	1 cut(s)	1738
Cac8I	GCNNGC	1 cut(s)	853
CciI	TCATGA	1 cut(s)	502
Cfr10I	RCCGGY	1 cut(s)	43
Cfr13I	GGNCC	6 cut(s)	79, 228, 673, 991, 1853, 2256
Cfr9I	CCCGGG	1 cut(s)	1018
ClaI	ATCGAT	1 cut(s)	64
CseI	GACGC	1 cut(s)	132
Csp6I	GTAC	2 cut(s)	1175, 1181
CspAI	ACCGGT	1 cut(s)	43
CviQI	GTAC	2 cut(s)	1175, 1181
DdeI	CTNAG	3 cut(s)	863, 1142, 1430
DraI	TTTAAA	2 cut(s)	1252, 1549
Eam1104I	CTCTTC	4 cut(s)	9, 18, 192, 2149
EarI	CTCTTC	4 cut(s)	9, 18, 192, 2149
Ecl136II	GAGCTC	1 cut(s)	28
Eco130I	CCWWGG	2 cut(s)	124, 1052
Eco24I	GRGCYC	1 cut(s)	30
Eco31I	GGTCTC	1 cut(s)	2012
Eco47I	GGWCC	5 cut(s)	79, 673, 991, 1853, 2256
Eco53kI	GAGCTC	1 cut(s)	28
Eco57I	CTGAAG	2 cut(s)	1520, 1725
Eco72I	CACGTG	1 cut(s)	1208
Eco88I	CYCGRG	3 cut(s)	1018, 2166, 2226
Eco91I	GGTNACC	1 cut(s)	154
EcoICRI	GAGCTC	1 cut(s)	28
EcoO109I	RGGNCCY	4 cut(s)	673, 991, 1853, 2256
EcoO65I	GGTNACC	1 cut(s)	154
EcoRI	GAATTC	3 cut(s)	253, 298, 1328
EcoRII	CCWGG	4 cut(s)	757, 1332, 2056, 2101
EcoT14I	CCWWGG	2 cut(s)	124, 1052
EcoT38I	GRGCYC	1 cut(s)	30
ErhI	CCWWGG	2 cut(s)	124, 1052
FalI	AAGNNNNNCTT	2 cut(s)	978, 1010
FaqI	GGGAC	6 cut(s)	1001, 1004, 1458, 1483, 1839, 1906
FauI	CCCGC	2 cut(s)	207, 234
FbaI	TGATCA	4 cut(s)	499, 1618, 1635, 1684
Fnu4HI	GCNGC	8 cut(s)	5, 141, 511, 726, 1559, 1867, 1983, 1986
FriOI	GRGCYC	1 cut(s)	30
Fsp4HI	GCNGC	8 cut(s)	5, 141, 511, 726, 1559, 1867, 1983, 1986
FspBI	CTAG	5 cut(s)	161, 1157, 1764, 1850, 1916
GluI	GCNGC	8 cut(s)	5, 141, 511, 726, 1559, 1867, 1983, 1986
GsaI	CCCAGC	1 cut(s)	1172
GsuI	CTGGAG	3 cut(s)	780, 2079, 2124
HaeIII	GGCC	2 cut(s)	230, 1500
HapII	CCGG	7 cut(s)	44, 470, 542, 663, 1019, 1487, 1505
HgaI	GACGC	1 cut(s)	132
Hin1I	GRCGYC	1 cut(s)	143
HincII	GTYRAC	1 cut(s)	1586
HindII	GTYRAC	1 cut(s)	1586
HindIII	AAGCTT	4 cut(s)	805, 822, 849, 1387
HpaII	CCGG	7 cut(s)	44, 470, 542, 663, 1019, 1487, 1505
HphI	GGTGA	5 cut(s)	452, 703, 1358, 1630, 1730
Hpy166II	GTNNAC	3 cut(s)	1115, 1175, 1586
Hpy188III	TCNNGA	5 cut(s)	274, 503, 590, 1121, 1703
Hpy8I	GTNNAC	3 cut(s)	1115, 1175, 1586
HpyCH4III	ACNGT	4 cut(s)	1185, 1474, 1513, 1936
HpyCH4IV	ACGT	3 cut(s)	1117, 1207, 2066
HpyF10VI	GCNNNNNNNGC	3 cut(s)	220, 286, 1807
HpyF3I	CTNAG	3 cut(s)	863, 1142, 1430
HpySE526I	ACGT	3 cut(s)	1117, 1207, 2066
Hsp92I	GRCGYC	1 cut(s)	143
Ksp22I	TGATCA	4 cut(s)	499, 1618, 1635, 1684
LguI	GCTCTTC	1 cut(s)	18
LmnI	GCTCC	5 cut(s)	220, 1592, 1830, 2060, 2105
Lsp1109I	GCAGC	5 cut(s)	16, 1570, 1853, 1969, 1972
LweI	GCATC	4 cut(s)	201, 1393, 1642, 2117
MaeI	CTAG	5 cut(s)	161, 1157, 1764, 1850, 1916
MaeII	ACGT	3 cut(s)	1117, 1207, 2066
MaeIII	GTNAC	3 cut(s)	154, 1414, 1990
MflI	RGATCY	7 cut(s)	270, 1195, 1426, 2053, 2098, 2158, 2221
MhlI	GDGCHC	2 cut(s)	30, 1117
MlyI	GAGTC	7 cut(s)	1708, 1727, 1928, 2035, 2125, 2140, 2203
MmeI	TCCRAC	1 cut(s)	622
MroXI	GAANNNNTTC	3 cut(s)	45, 65, 806
MspA1I	CMGCKG	1 cut(s)	1168
MspCI	CTTAAG	1 cut(s)	1744
MspI	CCGG	7 cut(s)	44, 470, 542, 663, 1019, 1487, 1505
MspR9I	CCNGG	8 cut(s)	663, 759, 1019, 1020, 1334, 1505, 2058, 2103
MvaI	CCWGG	4 cut(s)	759, 1334, 2058, 2103
MvnI	CGCG	1 cut(s)	104
MwoI	GCNNNNNNNGC	3 cut(s)	220, 286, 1807
NciI	CCSGG	4 cut(s)	663, 1019, 1020, 1505
NlaIV	GGNNCC	5 cut(s)	675, 992, 1832, 1855, 2258
NmeAIII	GCCGAG	1 cut(s)	1859
NspI	RCATGY	2 cut(s)	1016, 1565
NspV	TTCGAA	2 cut(s)	236, 302
PagI	TCATGA	1 cut(s)	502
PciSI	GCTCTTC	1 cut(s)	18
PcsI	WCGNNNNNNNCGW	1 cut(s)	1123
PdmI	GAANNNNTTC	3 cut(s)	45, 65, 806
PfeI	GAWTC	6 cut(s)	61, 66, 172, 1088, 1106, 1967
PflMI	CCANNNNNTGG	1 cut(s)	2183
PfoI	TCCNGGA	4 cut(s)	1332, 1503, 2056, 2101
PinAI	ACCGGT	1 cut(s)	43
PkrI	GCNGC	8 cut(s)	6, 142, 512, 727, 1560, 1868, 1984, 1987
PleI	GAGTC	7 cut(s)	1707, 1727, 1927, 2035, 2125, 2140, 2203
PmaCI	CACGTG	1 cut(s)	1208
PmlI	CACGTG	1 cut(s)	1208
PpsI	GAGTC	7 cut(s)	1707, 1727, 1927, 2035, 2125, 2140, 2203
Ppu21I	YACGTR	1 cut(s)	1208
PpuMI	RGGWCCY	4 cut(s)	673, 991, 1853, 2256
PsiI	TTATAA	1 cut(s)	189
Psp124BI	GAGCTC	1 cut(s)	30
Psp1406I	AACGTT	1 cut(s)	2066
Psp5II	RGGWCCY	4 cut(s)	673, 991, 1853, 2256
Psp6I	CCWGG	4 cut(s)	757, 1332, 2056, 2101
PspCI	CACGTG	1 cut(s)	1208
PspEI	GGTNACC	1 cut(s)	154
PspFI	CCCAGC	1 cut(s)	1168
PspGI	CCWGG	4 cut(s)	757, 1332, 2056, 2101
PspN4I	GGNNCC	5 cut(s)	675, 992, 1832, 1855, 2258
PspPI	GGNCC	6 cut(s)	79, 228, 673, 991, 1853, 2256
PspPPI	RGGWCCY	4 cut(s)	673, 991, 1853, 2256
PstI	CTGCAG	3 cut(s)	882, 1871, 1990
PsuI	RGATCY	7 cut(s)	270, 1195, 1426, 2053, 2098, 2158, 2221
PvuII	CAGCTG	1 cut(s)	1168
RsaI	GTAC	2 cut(s)	1176, 1182
RsaNI	GTAC	2 cut(s)	1175, 1181
SacI	GAGCTC	1 cut(s)	30
SapI	GCTCTTC	1 cut(s)	18
SatI	GCNGC	8 cut(s)	5, 141, 511, 726, 1559, 1867, 1983, 1986
Sau96I	GGNCC	6 cut(s)	79, 228, 673, 991, 1853, 2256
SchI	GAGTC	7 cut(s)	1708, 1727, 1928, 2035, 2125, 2140, 2203
ScrFI	CCNGG	8 cut(s)	663, 759, 1019, 1020, 1334, 1505, 2058, 2103
SduI	GDGCHC	2 cut(s)	30, 1117
SfaNI	GCATC	4 cut(s)	201, 1393, 1642, 2117
SfcI	CTRYAG	4 cut(s)	399, 878, 1867, 1986
SfuI	TTCGAA	2 cut(s)	236, 302
SinI	GGWCC	5 cut(s)	79, 673, 991, 1853, 2256
SmaI	CCCGGG	1 cut(s)	1020
SmlI	CTYRAG	2 cut(s)	1744, 2092
SmoI	CTYRAG	2 cut(s)	1744, 2092
SspMI	CTAG	5 cut(s)	161, 1157, 1764, 1850, 1916
SstI	GAGCTC	1 cut(s)	30
StyD4I	CCNGG	8 cut(s)	661, 757, 1017, 1018, 1332, 1503, 2056, 2101
StyI	CCWWGG	2 cut(s)	124, 1052
TaaI	ACNGT	4 cut(s)	1185, 1474, 1513, 1936
TaiI	ACGT	3 cut(s)	1120, 1210, 2069
TaqI	TCGA	8 cut(s)	59, 64, 236, 251, 296, 302, 377, 1094
TatI	WGTACW	1 cut(s)	1180
TauI	GCSGC	3 cut(s)	143, 513, 728
TfiI	GAWTC	6 cut(s)	61, 66, 172, 1088, 1106, 1967
TscAI	CASTG	1 cut(s)	1745
TseI	GCWGC	5 cut(s)	4, 1558, 1866, 1982, 1985
TspGWI	ACGGA	3 cut(s)	650, 1433, 1835
TspMI	CCCGGG	1 cut(s)	1018
TspRI	CASTG	1 cut(s)	1745
Van91I	CCANNNNNTGG	1 cut(s)	2183
Vha464I	CTTAAG	1 cut(s)	1744
VneI	GTGCAC	1 cut(s)	1113
VpaK11BI	GGWCC	5 cut(s)	79, 673, 991, 1853, 2256
XapI	RAATTY	3 cut(s)	253, 298, 1328
XceI	RCATGY	2 cut(s)	1016, 1565
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1340
XmaI	CCCGGG	1 cut(s)	1018
XmnI	GAANNNNTTC	3 cut(s)	45, 65, 806
XspI	CTAG	5 cut(s)	161, 1157, 1764, 1850, 1916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.