Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Forward (+)

16899695 .. 16913781

Gene Structure 14087 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027924

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2757 bp

ATGGAGCCTCATCGACACTCGGACCCCTACGATCCTCGACGCCACGCAGCCAGAAGAAGAGGACGAAGAGCTCTACGAATACGAACCGGTTCGGCTTGGCTCGAACCGATTCGTAATCGTGGTCCTCCTCAATCTCCTTCTTCTCGCGGTTTTTCTCATTCTTATCATTGGATTGGGCTTGGCGGCGTGATGCTTGCGAGAGTCATGTCGAATTGGAGAGAGGATTATGAAGAAGACGAGAGCGAGATGCGGGAGGAGAGGAGAGGAGGAGGACGAGGGGGGAATTCGAAGCGAGGGAGATTGAATTCTGACGAGATTGAGATCCAGAAGCGGAGAGGGCGAGGGTCGAATTCGAAAAAGAGGAAATTGAAATCGGTTGCGGAGGAGGAGGAGGAAGAAGAAGAAGAAGGCGGTGGTGGAAGGCCTTTTAAGTCGATGTTTGTGGATGAGGAGGAAGACGATGAGGAATATGAGGATGAAACAGAGGTTGGACAGCAAGCTCTTATACCCGGTGTTAAGGACCCGAAATTGTGGTTGGTGAAGTCTGTAGAATCATCGAAGAAGAAGGAATCTGGTGCACGTCGTGATCGTGTTCCTGCACTTCTTAGGAACATGAACCTAGTTAGACAGCTGGGTGTACGAGTACAGTCCATGCGAGATCCATTGGCATGTGGTTATATTGAGAAGATTGATGGCCTATCTGGGATGATCTTTAAAGATGGTTTCCATCTTAAAGTTGTTTCTTATAAAAGTATAAAAGAGGCAAAACCCTCCTTGCAAGAACTTGAAGAATTCCAGGAACTTGATGGTGATGGAGATCATACAATTCCAAATAGAAAGAAAGGCGAAAGCTTTATGAAGGGTGATGTGGTTGTTGTTATGGAGGGAGATCTGAGAAACTTGGTGGGACGGGTTGACAAAGTTGAGTTAGGGACTGTTCATATCAAACCGGAAATGGATGGCCTCCCAGAAACTGTTGCCACCAATCAAACAGCACTTCAGAAATGCTTTAAAGAAGGACAGCATGTGAAGGTTGTAAGTGGTAGTCAACAAGGAGCAACTGGCATGGTTGTTAAGGTTGATCAGCAGAAGCAGTTGATCACCATTTTATCTGATGCAGCCAATGAAGCTATCTGCGTGTTATCTGATCATGTTGTGGAGAGTCCTGAAGTGAATAATGGTGATACAATGGGTGACTCCCACAGCTTAAGGTATCACAAAACCCCTGGTCGAAGTCCGAGGTTTTCTGGAGGTGCAAGAAGGCATGATGGGCAGGGTGGTTTTGGTAGAGGGAGCCAAGTAAAATTCCGGCTAGGTCCCTATAAGGGCTGCAGGGGGCGTGTTGTGGAGGTTAAAGGATCAAATGTAAGGGTTGAATTAGAGTCCCAGATGAAAACTGTTGTTGTTGACCGTATGCATTGTATTTCAGACTCTAGTAACGTGGCTGCTGCAGTAACAACACCATATTGCTCTGGTTGTGAGACCCCAATGCCTTACATGAGGACTCCTATGAGAGATCCTGGAGCAATGTCAATGACCTACATGACGACTCCCATGAGAGATCCCGGAGCATCCCCAATGCGTTATATGATGACTCCCAGAAGAGACCCCCCCCCCCCGAGCAACACCAATACATGGTGGCATGAGAACTCCACCTATGACTCCCATGAGAGATCCCCGAGCAACACCAATGCATGGTGGCATGAGGACCCCTCCTATATATTAGAGCTTGATTCAACTTTTGGTTTATCAAATGATGGTTGTCCGGTTCATGAAAGTTTGCAGAGGATACGTGAAAGAAAGCTTCTTAACTTTATTGAGTGGGCCCCTGCAAGCATTCAGGTTGCGTTGTCTAGGAAGTCTCCATCTGTGCAAACTGGCCATAGGGTCAGTGGTCTTATGCTAGCCAGCCATACTAGTATTCGCCACCTTTTCAGCAAGTGTTTGAGCCAGTATTCGTTGGTAAGAAGGAAGCAAGCTTTTCTTGACAACTACAGGAAGTTCCCAATGTTTGCTGAGAATGACCTTTCAGAATTTCATGAATCAACCACGTTCTTACAGGAGAAGGCAGTGCAGCAGGAACAGTGGACCCAAAAGTGGCAGTATGAACAATTTAGAAAGAAGGAGAAGAGTGATTTGATTTTGGGTTTGTTTTGGCTTGGTCGGAGGAATTTGAGGTTTACTGGTCGGGTTGGAAGGGGATGTGCAAGAAGAAGAGGTTCTCTGCAACGGAGTATTTGGATTGTTCAGTTGTCCAAAGGTGAGCTGCTGCAACTAAACCAATCGTGCTTGGGTTTTGAAGATAATCGTGTTAGATCAACCAGGTTGTGCATATTCAAGGGGAAGAGGGGCTTCTCATCGGCTATGGAGCTTCAATACAGACAGAGACTGCAGAAAGAGAAGATTGGGTTGAAAACAAGTGGAACAGATAGAGGTTTCTGGGCGGTTGGTTGGCTGATTGGGGAAAACGGTGGAGCTGCAGACGCCAAAAATCTAAGGGTGAAGATTATGGTTTTAAGTTTTGGTGTTGGCATAGAGAGAAATGCACATGGATTGAAATTTTCTACTTACTGCTCTGGTTTTGTAGTGCTTTTCAAGGAATTCATACTGGAGTTTTTATTATTATTACAACACCTGGCAGGTCATTTTCTGCTGACCAATCTCCTGTTGAAGACCATGAAAATTTACTGGCACCTCCCACATTTCGGGCATGATATTCATAAGGTAAAGGAGTTTCAGTCTAGCAAAAGTTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

919

Amino Acids

104.4

Weight (kDa)

9.38

Isoelectric Point (pI)

51.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KOW1_SPT5	PF23042	199 - 265	2.9e-09	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	287 - 336	1.1e-14	Transcription elongation factor SPT5, second KOW domain
KOW5_SPT5	PF23290	431 - 473	7.1e-13	Transcription elongation factor SPT5, fifth KOW domain
Tubulin_C	PF03953	590 - 648	2.3e-16	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	747
Acc36I	ACCTGC	1 cut(s)	2630
AccB1I	GGYRCC	1 cut(s)	2691
AccB7I	CCANNNNNTGG	2 cut(s)	1635, 1695
AccII	CGCG	1 cut(s)	147
AciI	CCGC	7 cut(s)	147, 183, 250, 331, 380, 411, 2444
AclWI	GGATC	7 cut(s)	26, 316, 653, 1366, 1511, 1556, 1668
AcoI	YGGCCR	1 cut(s)	1879
AcuI	CTGAAG	2 cut(s)	983, 1188
AcyI	GRCGYC	2 cut(s)	40, 2484
AdeI	CACNNNGTG	1 cut(s)	584
AfaI	GTAC	2 cut(s)	639, 645
AfiI	CCNNNNNNNGG	5 cut(s)	1325, 1635, 1695, 2097, 2705
AflII	CTTAAG	1 cut(s)	1207
AgeI	ACCGGT	1 cut(s)	86
AhlI	ACTAGT	1 cut(s)	1916
AjiI	CACGTC	1 cut(s)	581
AjnI	CCWGG	5 cut(s)	795, 1225, 1519, 2321, 2634
AjuI	GAANNNNNNNTTGG	4 cut(s)	471, 503, 518, 550
Alw21I	GWGCWC	2 cut(s)	73, 580
Alw26I	GTCTC	4 cut(s)	1475, 1599, 1866, 2380
Alw44I	GTGCAC	1 cut(s)	576
AlwI	GGATC	7 cut(s)	26, 316, 653, 1366, 1511, 1556, 1668
AlwNI	CAGNNNCTG	2 cut(s)	974, 2389
Ama87I	CYCGRG	2 cut(s)	1618, 1678
AoxI	GGCC	5 cut(s)	422, 694, 961, 1824, 1879
ApaI	GGGCCC	1 cut(s)	1828
ApaLI	GTGCAC	1 cut(s)	576
ApeKI	GCWGC	9 cut(s)	47, 1118, 1329, 1445, 1448, 2074, 2266, 2269, 2477
AsiGI	ACCGGT	1 cut(s)	86
Asp700I	GAANNNNTTC	3 cut(s)	88, 108, 2218
AspS9I	GGNCC	8 cut(s)	22, 122, 520, 1316, 1708, 1824, 1825, 2088
AsuC2I	CCSGG	2 cut(s)	510, 1566
AsuHPI	GGTGA	8 cut(s)	550, 821, 875, 1093, 1193, 1205, 2273, 2512
AsuII	TTCGAA	2 cut(s)	287, 353
AsuNHI	GCTAGC	1 cut(s)	1903
AvaI	CYCGRG	2 cut(s)	1618, 1678
AvaII	GGWCC	6 cut(s)	22, 122, 520, 1316, 1708, 2088
BaeGI	GKGCMC	2 cut(s)	580, 1828
BalI	TGGCCA	1 cut(s)	1881
BanI	GGYRCC	1 cut(s)	2691
BanII	GRGCYC	2 cut(s)	73, 1828
BbsI	GAAGAC	3 cut(s)	240, 462, 2678
Bbv12I	GWGCWC	2 cut(s)	73, 580
BbvI	GCAGC	9 cut(s)	59, 1130, 1316, 1432, 1435, 2086, 2253, 2256, 2464
BccI	CCATC	9 cut(s)	686, 713, 735, 800, 806, 953, 1262, 1751, 1873
BciT130I	CCWGG	5 cut(s)	797, 1227, 1521, 2323, 2636
BciVI	GTATCC	1 cut(s)	1782
BclI	TGATCA	3 cut(s)	1081, 1098, 1147
BcnI	CCSGG	2 cut(s)	510, 1566
BcoDI	GTCTC	4 cut(s)	1475, 1599, 1866, 2380
BcuI	ACTAGT	1 cut(s)	1916
BfaI	CTAG	7 cut(s)	620, 1313, 1434, 1854, 1904, 1917, 2742
BfmI	CTRYAG	6 cut(s)	546, 1330, 1449, 1993, 2390, 2478
BfrI	CTTAAG	1 cut(s)	1207
BfuAI	ACCTGC	1 cut(s)	2630
BfuI	GTATCC	1 cut(s)	1782
BglII	AGATCT	1 cut(s)	889
Bme1390I	CCNGG	7 cut(s)	510, 797, 1227, 1521, 1566, 2323, 2636
Bme18I	GGWCC	6 cut(s)	22, 122, 520, 1316, 1708, 2088
BmeT110I	CYCGRG	2 cut(s)	1618, 1678
BmgBI	CACGTC	1 cut(s)	581
BmgT120I	GGNCC	8 cut(s)	22, 122, 520, 1316, 1708, 1824, 1825, 2088
BmrFI	CCNGG	7 cut(s)	510, 797, 1227, 1521, 1566, 2323, 2636
BmsI	GCATC	4 cut(s)	180, 237, 1105, 1580
BmtI	GCTAGC	1 cut(s)	1907
BpiI	GAAGAC	3 cut(s)	240, 462, 2678
BplI	GAGNNNNNCTC	2 cut(s)	1697, 1729
BpmI	CTGGAG	3 cut(s)	1269, 1542, 2630
Bpu14I	TTCGAA	2 cut(s)	287, 353
BpuMI	CCSGG	2 cut(s)	510, 1566
BsaAI	YACGTR	1 cut(s)	1793
BsaBI	GATNNNNATC	2 cut(s)	320, 816
BsaHI	GRCGYC	2 cut(s)	40, 2484
BsaI	GGTCTC	2 cut(s)	1475, 1599
BsaJI	CCNNGG	2 cut(s)	1225, 1238
BsaWI	WCCGGW	3 cut(s)	86, 949, 1765
BsaXI	ACNNNNNCTCC	2 cut(s)	2723, 2753
Bsc4I	CCNNNNNNNGG	5 cut(s)	1325, 1635, 1695, 2097, 2705
Bse118I	RCCGGY	1 cut(s)	86
Bse1I	ACTGG	6 cut(s)	1066, 1882, 1951, 2188, 2613, 2693
Bse3DI	GCAATG	1 cut(s)	1533
Bse8I	GATNNNNATC	2 cut(s)	320, 816
BseBI	CCWGG	5 cut(s)	797, 1227, 1521, 2323, 2636
BseDI	CCNNGG	2 cut(s)	1225, 1238
BseGI	GGATG	6 cut(s)	451, 481, 711, 964, 1571, 2207
BseJI	GATNNNNATC	2 cut(s)	320, 816
BseLI	CCNNNNNNNGG	5 cut(s)	1325, 1635, 1695, 2097, 2705
BseMI	GCAATG	1 cut(s)	1533
BseMII	CTCAG	2 cut(s)	884, 2007
BseNI	ACTGG	6 cut(s)	1066, 1882, 1951, 2188, 2613, 2693
BseRI	GAGGAG	9 cut(s)	117, 269, 274, 279, 282, 398, 401, 404, 464
BseSI	GKGCMC	2 cut(s)	580, 1828
BseXI	GCAGC	9 cut(s)	59, 1130, 1316, 1432, 1435, 2086, 2253, 2256, 2464
BseYI	CCCAGC	1 cut(s)	631
BsgI	GTGCAG	2 cut(s)	582, 2093
Bsh1236I	CGCG	1 cut(s)	147
BshFI	GGCC	5 cut(s)	424, 696, 963, 1826, 1881
BshNI	GGYRCC	1 cut(s)	2691
BshTI	ACCGGT	1 cut(s)	86
BsiHKAI	GWGCWC	2 cut(s)	73, 580
BsiHKCI	CYCGRG	2 cut(s)	1618, 1678
BsiSI	CCGG	6 cut(s)	87, 510, 950, 1309, 1566, 1766
BslFI	GGGAC	4 cut(s)	921, 946, 1302, 1369
BslI	CCNNNNNNNGG	5 cut(s)	1325, 1635, 1695, 2097, 2705
BsmAI	GTCTC	4 cut(s)	1475, 1599, 1866, 2380
BsmFI	GGGAC	4 cut(s)	921, 946, 1302, 1369
BsmI	GAATGC	1 cut(s)	1836
BsnI	GGCC	5 cut(s)	424, 696, 963, 1826, 1881
Bso31I	GGTCTC	2 cut(s)	1475, 1599
BsoBI	CYCGRG	2 cut(s)	1618, 1678
Bsp119I	TTCGAA	2 cut(s)	287, 353
Bsp120I	GGGCCC	1 cut(s)	1824
Bsp1286I	GDGCHC	3 cut(s)	73, 580, 1828
BspACI	CCGC	7 cut(s)	147, 183, 250, 331, 380, 411, 2444
BspANI	GGCC	5 cut(s)	424, 696, 963, 1826, 1881
BspCNI	CTCAG	2 cut(s)	885, 2008
BspFNI	CGCG	1 cut(s)	147
BspHI	TCATGA	2 cut(s)	1771, 2038
BspMAI	CTGCAG	4 cut(s)	1334, 1453, 2394, 2482
BspMI	ACCTGC	1 cut(s)	2630
BspOI	GCTAGC	1 cut(s)	1907
BspPI	GGATC	7 cut(s)	26, 316, 653, 1366, 1511, 1556, 1668
BspQI	GCTCTTC	1 cut(s)	61
BspT104I	TTCGAA	2 cut(s)	287, 353
BspT107I	GGYRCC	1 cut(s)	2691
BspTI	CTTAAG	1 cut(s)	1207
BspTNI	GGTCTC	2 cut(s)	1475, 1599
BsrDI	GCAATG	1 cut(s)	1533
BsrFI	RCCGGY	1 cut(s)	86
BsrI	ACTGG	6 cut(s)	1066, 1882, 1951, 2188, 2613, 2693
BssAI	RCCGGY	1 cut(s)	86
BssECI	CCNNGG	2 cut(s)	1225, 1238
BssNI	GRCGYC	2 cut(s)	40, 2484
Bst2UI	CCWGG	5 cut(s)	797, 1227, 1521, 2323, 2636
Bst4CI	ACNGT	7 cut(s)	648, 937, 976, 1399, 1412, 2085, 2471
Bst6I	CTCTTC	6 cut(s)	52, 61, 1597, 2123, 2209, 2339
BstACI	GRCGYC	2 cut(s)	40, 2484
BstAFI	CTTAAG	1 cut(s)	1207
BstBAI	YACGTR	1 cut(s)	1793
BstBI	TTCGAA	2 cut(s)	287, 353
BstC8I	GCNNGC	6 cut(s)	195, 498, 1834, 1905, 1909, 1977
BstDEI	CTNAG	4 cut(s)	605, 893, 2016, 2495
BstF5I	GGATG	6 cut(s)	451, 481, 711, 964, 1571, 2207
BstFNI	CGCG	1 cut(s)	147
BstMAI	GTCTC	4 cut(s)	1475, 1599, 1866, 2380
BstMWI	GCNNNNNNNGC	4 cut(s)	337, 1127, 1270, 2483
BstNI	CCWGG	5 cut(s)	797, 1227, 1521, 2323, 2636
BstNSI	RCATGY	2 cut(s)	672, 1028
BstSCI	CCNGG	7 cut(s)	508, 795, 1225, 1519, 1564, 2321, 2634
BstSFI	CTRYAG	6 cut(s)	546, 1330, 1449, 1993, 2390, 2478
BstSLI	GKGCMC	2 cut(s)	580, 1828
BstUI	CGCG	1 cut(s)	147
BstV1I	GCAGC	9 cut(s)	59, 1130, 1316, 1432, 1435, 2086, 2253, 2256, 2464
BstV2I	GAAGAC	3 cut(s)	240, 462, 2678
BstX2I	RGATCY	6 cut(s)	321, 658, 889, 1516, 1561, 1673
BstYI	RGATCY	6 cut(s)	321, 658, 889, 1516, 1561, 1673
BsuI	GTATCC	1 cut(s)	1782
BsuRI	GGCC	5 cut(s)	424, 696, 963, 1826, 1881
BtrI	CACGTC	1 cut(s)	581
BtsCI	GGATG	6 cut(s)	451, 481, 711, 964, 1571, 2207
BtsI	GCAGTG	1 cut(s)	2076
BtsIMutI	CAGTG	3 cut(s)	1897, 2076, 2090
BveI	ACCTGC	1 cut(s)	2630
Cac8I	GCNNGC	6 cut(s)	195, 498, 1834, 1905, 1909, 1977
CaiI	CAGNNNCTG	2 cut(s)	974, 2389
CciI	TCATGA	2 cut(s)	1771, 2038
Cfr10I	RCCGGY	1 cut(s)	86
Cfr13I	GGNCC	8 cut(s)	22, 122, 520, 1316, 1708, 1824, 1825, 2088
CseI	GACGC	2 cut(s)	48, 2492
CsiI	ACCWGGT	1 cut(s)	2321
Csp6I	GTAC	2 cut(s)	638, 644
CspAI	ACCGGT	1 cut(s)	86
CviQI	GTAC	2 cut(s)	638, 644
DdeI	CTNAG	4 cut(s)	605, 893, 2016, 2495
DraI	TTTAAA	2 cut(s)	715, 1012
DraIII	CACNNNGTG	1 cut(s)	584
EaeI	YGGCCR	1 cut(s)	1879
Eam1104I	CTCTTC	6 cut(s)	52, 61, 1597, 2123, 2209, 2339
EarI	CTCTTC	6 cut(s)	52, 61, 1597, 2123, 2209, 2339
Ecl136II	GAGCTC	1 cut(s)	71
Eco147I	AGGCCT	1 cut(s)	424
Eco24I	GRGCYC	2 cut(s)	73, 1828
Eco31I	GGTCTC	2 cut(s)	1475, 1599
Eco47I	GGWCC	6 cut(s)	22, 122, 520, 1316, 1708, 2088
Eco53kI	GAGCTC	1 cut(s)	71
Eco57I	CTGAAG	2 cut(s)	983, 1188
Eco88I	CYCGRG	2 cut(s)	1618, 1678
EcoICRI	GAGCTC	1 cut(s)	71
EcoO109I	RGGNCCY	4 cut(s)	520, 1316, 1708, 1825
EcoRI	GAATTC	5 cut(s)	283, 304, 349, 791, 2600
EcoRII	CCWGG	5 cut(s)	795, 1225, 1519, 2321, 2634
EcoT22I	ATGCAT	2 cut(s)	1419, 1696
EcoT38I	GRGCYC	2 cut(s)	73, 1828
FalI	AAGNNNNNCTT	4 cut(s)	1968, 2000, 2336, 2368
FaqI	GGGAC	4 cut(s)	921, 946, 1302, 1369
FauI	CCCGC	1 cut(s)	243
FbaI	TGATCA	3 cut(s)	1081, 1098, 1147
FokI	GGATG	6 cut(s)	458, 488, 718, 971, 1558, 2214
FriOI	GRGCYC	2 cut(s)	73, 1828
FspBI	CTAG	7 cut(s)	620, 1313, 1434, 1854, 1904, 1917, 2742
GsaI	CCCAGC	1 cut(s)	635
GsuI	CTGGAG	3 cut(s)	1269, 1542, 2630
HaeIII	GGCC	5 cut(s)	424, 696, 963, 1826, 1881
HapII	CCGG	6 cut(s)	87, 510, 950, 1309, 1566, 1766
HgaI	GACGC	2 cut(s)	48, 2492
Hin1I	GRCGYC	2 cut(s)	40, 2484
HincII	GTYRAC	3 cut(s)	916, 1049, 1408
HindII	GTYRAC	3 cut(s)	916, 1049, 1408
HindIII	AAGCTT	3 cut(s)	850, 1802, 1977
HpaII	CCGG	6 cut(s)	87, 510, 950, 1309, 1566, 1766
HphI	GGTGA	8 cut(s)	550, 821, 875, 1093, 1193, 1205, 2273, 2512
Hpy166II	GTNNAC	7 cut(s)	578, 638, 916, 1049, 1408, 2088, 2181
Hpy188III	TCNNGA	7 cut(s)	325, 584, 1166, 1248, 1772, 1985, 2039
Hpy8I	GTNNAC	7 cut(s)	578, 638, 916, 1049, 1408, 2088, 2181
Hpy99I	CGWCG	2 cut(s)	42, 585
HpyCH4III	ACNGT	7 cut(s)	648, 937, 976, 1399, 1412, 2085, 2471
HpyCH4IV	ACGT	4 cut(s)	580, 1440, 1792, 2051
HpyF10VI	GCNNNNNNNGC	4 cut(s)	337, 1127, 1270, 2483
HpyF3I	CTNAG	4 cut(s)	605, 893, 2016, 2495
HpySE526I	ACGT	4 cut(s)	580, 1440, 1792, 2051
Hsp92I	GRCGYC	2 cut(s)	40, 2484
Ksp22I	TGATCA	3 cut(s)	1081, 1098, 1147
LguI	GCTCTTC	1 cut(s)	61
LmnI	GCTCC	7 cut(s)	4, 1055, 1293, 1523, 1568, 2368, 2474
Lsp1109I	GCAGC	9 cut(s)	59, 1130, 1316, 1432, 1435, 2086, 2253, 2256, 2464
LweI	GCATC	4 cut(s)	180, 237, 1105, 1580
MabI	ACCWGGT	1 cut(s)	2321
MaeI	CTAG	7 cut(s)	620, 1313, 1434, 1854, 1904, 1917, 2742
MaeII	ACGT	4 cut(s)	580, 1440, 1792, 2051
MaeIII	GTNAC	3 cut(s)	1193, 1436, 1453
MflI	RGATCY	6 cut(s)	321, 658, 889, 1516, 1561, 1673
MhlI	GDGCHC	3 cut(s)	73, 580, 1828
MlsI	TGGCCA	1 cut(s)	1881
MluNI	TGGCCA	1 cut(s)	1881
MlyI	GAGTC	9 cut(s)	210, 1171, 1190, 1391, 1424, 1498, 1543, 1588, 1655
MmeI	TCCRAC	3 cut(s)	469, 2144, 2173
Mox20I	TGGCCA	1 cut(s)	1881
Mph1103I	ATGCAT	2 cut(s)	1419, 1696
MroXI	GAANNNNTTC	3 cut(s)	88, 108, 2218
MscI	TGGCCA	1 cut(s)	1881
MslI	CAYNNNNRTG	1 cut(s)	667
Msp20I	TGGCCA	1 cut(s)	1881
MspA1I	CMGCKG	1 cut(s)	631
MspCI	CTTAAG	1 cut(s)	1207
MspI	CCGG	6 cut(s)	87, 510, 950, 1309, 1566, 1766
MspR9I	CCNGG	7 cut(s)	510, 797, 1227, 1521, 1566, 2323, 2636
Mva1269I	GAATGC	1 cut(s)	1836
MvaI	CCWGG	5 cut(s)	797, 1227, 1521, 2323, 2636
MvnI	CGCG	1 cut(s)	147
MwoI	GCNNNNNNNGC	4 cut(s)	337, 1127, 1270, 2483
NciI	CCSGG	2 cut(s)	510, 1566
NheI	GCTAGC	1 cut(s)	1903
NmuCI	GTSAC	1 cut(s)	1193
NsiI	ATGCAT	2 cut(s)	1419, 1696
NspI	RCATGY	2 cut(s)	672, 1028
NspV	TTCGAA	2 cut(s)	287, 353
PagI	TCATGA	2 cut(s)	1771, 2038
PceI	AGGCCT	1 cut(s)	424
PciSI	GCTCTTC	1 cut(s)	61
PcsI	WCGNNNNNNNCGW	1 cut(s)	586
PctI	GAATGC	1 cut(s)	1836
PdmI	GAANNNNTTC	3 cut(s)	88, 108, 2218
PfeI	GAWTC	5 cut(s)	109, 551, 569, 1733, 2042
PflMI	CCANNNNNTGG	2 cut(s)	1635, 1695
PfoI	TCCNGGA	3 cut(s)	795, 1519, 1564
PinAI	ACCGGT	1 cut(s)	86
PleI	GAGTC	9 cut(s)	209, 1170, 1190, 1390, 1424, 1498, 1543, 1588, 1655
PpsI	GAGTC	9 cut(s)	209, 1170, 1190, 1390, 1424, 1498, 1543, 1588, 1655
Ppu21I	YACGTR	1 cut(s)	1793
PpuMI	RGGWCCY	3 cut(s)	520, 1316, 1708
PsiI	TTATAA	1 cut(s)	747
Psp124BI	GAGCTC	1 cut(s)	73
Psp5II	RGGWCCY	3 cut(s)	520, 1316, 1708
Psp6I	CCWGG	5 cut(s)	795, 1225, 1519, 2321, 2634
PspFI	CCCAGC	1 cut(s)	631
PspGI	CCWGG	5 cut(s)	795, 1225, 1519, 2321, 2634
PspOMI	GGGCCC	1 cut(s)	1824
PspPI	GGNCC	8 cut(s)	22, 122, 520, 1316, 1708, 1824, 1825, 2088
PspPPI	RGGWCCY	3 cut(s)	520, 1316, 1708
PstI	CTGCAG	4 cut(s)	1334, 1453, 2394, 2482
PstNI	CAGNNNCTG	2 cut(s)	974, 2389
PsuI	RGATCY	6 cut(s)	321, 658, 889, 1516, 1561, 1673
PvuII	CAGCTG	1 cut(s)	631
RsaI	GTAC	2 cut(s)	639, 645
RsaNI	GTAC	2 cut(s)	638, 644
RseI	CAYNNNNRTG	1 cut(s)	667
SacI	GAGCTC	1 cut(s)	73
SapI	GCTCTTC	1 cut(s)	61
Sau96I	GGNCC	8 cut(s)	22, 122, 520, 1316, 1708, 1824, 1825, 2088
SchI	GAGTC	9 cut(s)	210, 1171, 1190, 1391, 1424, 1498, 1543, 1588, 1655
ScrFI	CCNGG	7 cut(s)	510, 797, 1227, 1521, 1566, 2323, 2636
SduI	GDGCHC	3 cut(s)	73, 580, 1828
SexAI	ACCWGGT	1 cut(s)	2321
SfaNI	GCATC	4 cut(s)	180, 237, 1105, 1580
SfcI	CTRYAG	6 cut(s)	546, 1330, 1449, 1993, 2390, 2478
SfuI	TTCGAA	2 cut(s)	287, 353
SinI	GGWCC	6 cut(s)	22, 122, 520, 1316, 1708, 2088
SmiMI	CAYNNNNRTG	1 cut(s)	667
SmlI	CTYRAG	1 cut(s)	1207
SmoI	CTYRAG	1 cut(s)	1207
SpeI	ACTAGT	1 cut(s)	1916
SseBI	AGGCCT	1 cut(s)	424
SsiI	CCGC	7 cut(s)	147, 183, 250, 331, 380, 411, 2444
SspMI	CTAG	7 cut(s)	620, 1313, 1434, 1854, 1904, 1917, 2742
SstI	GAGCTC	1 cut(s)	73
StuI	AGGCCT	1 cut(s)	424
StyD4I	CCNGG	7 cut(s)	508, 795, 1225, 1519, 1564, 2321, 2634
TaaI	ACNGT	7 cut(s)	648, 937, 976, 1399, 1412, 2085, 2471
TaiI	ACGT	4 cut(s)	583, 1443, 1795, 2054
TatI	WGTACW	1 cut(s)	643
TauI	GCSGC	1 cut(s)	186
TfiI	GAWTC	5 cut(s)	109, 551, 569, 1733, 2042
TscAI	CASTG	3 cut(s)	1897, 2076, 2090
TseFI	GTSAC	1 cut(s)	1193
TseI	GCWGC	9 cut(s)	47, 1118, 1329, 1445, 1448, 2074, 2266, 2269, 2477
Tsp45I	GTSAC	1 cut(s)	1193
TspGWI	ACGGA	1 cut(s)	2245
TspRI	CASTG	3 cut(s)	1897, 2076, 2090
Van91I	CCANNNNNTGG	2 cut(s)	1635, 1695
Vha464I	CTTAAG	1 cut(s)	1207
VneI	GTGCAC	1 cut(s)	576
VpaK11BI	GGWCC	6 cut(s)	22, 122, 520, 1316, 1708, 2088
XceI	RCATGY	2 cut(s)	672, 1028
XcmI	CCANNNNNNNNNTGG	2 cut(s)	803, 1889
XmnI	GAANNNNTTC	3 cut(s)	88, 108, 2218
XspI	CTAG	7 cut(s)	620, 1313, 1434, 1854, 1904, 1917, 2742
Zsp2I	ATGCAT	2 cut(s)	1419, 1696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.