Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Reverse (-)

42064697 .. 42071303

Gene Structure 6607 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG222000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2733 bp

ATGCATCATCGCCCATTGATGGATGACCAAGAGCAAGAAGATGTCGAGGCTCTGGAAAGAAGAATTCAGGCAAGATATGCAAAGCAACATCATACAGAAGATACTGAGGAGACCACTGATGTGGATCAGCAAGCTCTTTTACCTTCTGTTTTGGACCCAAAGCTGTGGATGGTGAAATGTGCGATTGGTAAAGAACGAGAGGTAGCTGCTTGCTTAATGCAAAAGTATATTGATAAACCTGAATTGAACATCAGGTCTGTTATTGCTCTTGACCACCTGAAGAACTATATATACGTTGAAGCGGAAAAAGAAGCCCACGTGAGGGAGGCTTGCAAAGGATTGCGCAATATATTTACCCAGAAAATAGCGCTTGTTCCTATTAGAGAAATGACTGATGTTCTTTCCGTTGAAAGCAAGGCAATAGATATTTCTAGGGATACTTGGGTCAGAATGAAGATTGGGATATATAAAGGGGACCTTGCCAAGGTTGTTGATGTGGATAATGTACGACAGAGAGTTACAGTAAAATTAATTCCAAGAATTGATTTACAAGCTCTTGCAAATAAACTGGAGGGCAGAGAAGTTGTCAAAAAGAAAGCGTTTGTGCCTCCGCCACGTTTTATGAATATTGATGAAGCTAGAGAACTGCATATTCGTGTGGAGCGTAAACGAGATCCAATGACGGGTGACTACTTTGAAAACATTGAGAAAATGATGTTTAAAGATGGTTTCTTGTATAAAATAGTATCAATGAAATCAATTAGTACTCAAAACATACATCCCACTTTTGATGAACTGGAAAAGTTTCGGAAACCTGGGGAGAATGGGGAAGGAGATGTTGCCAGTCTGTCAACTTTATTTTCAAACAGAAAGAAAGGACACTTTGTGAAGGGTGATGCAGTCATTGTCATCAAGGGAGACCTAAAAAACTTGAAAGGGCGGGTTGAGAAAGTTGAGGAAGGGACGGTGCATATCAGACCTGACATGAAAGACCTCCCAAAAACTCTTGCCATAAATGAAAAAGAACTTTGCAAGTACTTTGAACCTGGGAATCATGTGAAGGTTGTATCTGGTACTCAAGAAGGTGCAACTGGTATGGTTGTCAAGGTCGATCAGCATGTGCTCATCATTTTATCTGATACAACTAAGGAACATCTCCGTGTGTTTGCTGATGATGTTGTGGAGAGTTCTGAGGTGACATCTGGTATTACCAAAATTGGGGACTATGAGCTTCATGATCTTGTGCTACTGGATAATAATAGCTTTGGTGTAATTATACGTGTTGAAACTGAAGCATGTCAGGTCCTTAAGGGAGTTCCTGAGAGACCTGAGGTTGCCCTTGTCAAATTAAGAGAGATCAAATGCAAGATTGAGAAAAAGCTTAGTGTGCAAGATTGTTTTAAGAACACAATATCTGTGAAAGATGTTGTGAGGGTCGTTGAAGGTCCTTCCAAGGGTAAACAAGGTCCTGTGGAACACATATACAGAGGAGTCTTATTCATTTATGATCGCCATCACATAGAGCATGCGGGCTTTATCTGTGTTAAGTCACATTCTTGTCGTGTTGTAGGAGGATCACGTGCCAATGGTGATAGAAATGGTGACTCCTACTCTAGATTTGATCATCTTCGAGCTCCTCCTCTTATTCCCCAGTCCCCCAGGAGGTTTCCTAGAGGAGGCCCTCCTAATAACTTTGGGGGAAGAAATAGAGGTGGAAGAGGGCATGATGGATTGGTTGGGACTACAGTAAAGATTCGCCAGGGTGCCTATAAGGGTTACCGTGGGCGCGTTGTTGAGGTTAAGGGAACTAGTGTACGGGTGGAACTTGAGTCCCAAATGAAAGTTGTGACAGTTGACCGGAATTGTATTTCTGATAACGTGGCTATTACAACACCGTATCGTGATACATCTAGCTATGGTATGGGAAGTCAGACCCCAATTCATCCTTCTCGAACTCCGCTCCATCCATATATGACGCCAATGAGGGATGCTGGAGCAACGCCTATTCATGATGGCATGAGGACTCCAATGCGTGACCGAGCATGGAATCCGTATGCACCTATGAGTCCAGCAAGGGATAGCTGGGAGGATGGAAACCCTGGTTCTTGGGGTTCTAGCCCACAGTATCAGCCAGGAAGTCCTCCCTCACGGACGTACGAAGCTCCAACTCCTGGTTCAGGTTGGGCCAACACTCCTGGTGGGAATTATAGTGAAGCTGGAACACCAAGGGACAGTACTGGTTATGCAAATGCTCCTAGCCCATACTTGCCGTCAACTCCCGGTGGGCAGCCTATGACACCAAACTCAGCATCCTATCTTCCCGGAACTCCTGGAGGGCAGCCAATGACACCAGGGACTGGTGGTCTGGATATGATGTCTCCTGTTATAGGAGGGGACAATGAAGGACCATGGTTCATGCCAGATATACTGGTCAATGTACGCAATTCTGGAGAGGAAACTACTGGAATTGTCAGAGAAGTGCTCCTGGATGGCTCATGTAGGGTAGCTCTTGGCTCGGGTGGCAATGGTGATACTGTAACTGTACTTCCAAATGAAATCGAGGTAGTCGTACCAAGGAAGAATGATAAGATCAAAATCATGGGGGGCTCATTACGCGGTGCCACTGGCAAGCTGATTGGTGTCGATGGCACGGATGGAATTGTCAAGGTTGATGACACTCTTGATGTTAAAATATTAGACTTGGCTATTTTGTCAAAACTAGGTCAATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

910

Amino Acids

100.54

Weight (kDa)

8.25

Isoelectric Point (pI)

34.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5-NGN	PF03439	54 - 134	6.8e-27	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	140 - 269	4.1e-76	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	297 - 346	3.5e-22	Transcription elongation factor SPT5, second KOW domain
KOWx_SPT5	PF23037	405 - 456	1.1e-17	Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5	PF23291	464 - 517	5.9e-24	Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5	PF23290	578 - 621	6.1e-18	Transcription elongation factor SPT5, fifth KOW domain
KOW6_SPT51-2	PF23038	805 - 854	5.2e-26	Transcription elongation factor SPT5 homolog 1/2, KOW6 domain
KOW7_SPT5	PF23287	856 - 907	3.2e-21	Transcription elongation factor SPT5, seventh KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	344
AccB1I	GGYRCC	2 cut(s)	1763, 2618
AccB7I	CCANNNNNTGG	2 cut(s)	2171, 2357
AccBSI	CCGCTC	1 cut(s)	1960
AccII	CGCG	2 cut(s)	1788, 2616
AciI	CCGC	6 cut(s)	302, 611, 940, 1529, 1958, 2616
AclWI	GGATC	3 cut(s)	132, 668, 1582
AcsI	RAATTY	1 cut(s)	63
AcuI	CTGAAG	2 cut(s)	299, 1311
AcvI	CACGTG	2 cut(s)	319, 1580
AcyI	GRCGYC	1 cut(s)	1976
AdeI	CACNNNGTG	1 cut(s)	886
AfeI	AGCGCT	1 cut(s)	369
AflII	CTTAAG	1 cut(s)	1307
AflIII	ACRYGT	1 cut(s)	1279
AgsI	TTSAA	9 cut(s)	247, 299, 410, 698, 864, 934, 1043, 1286, 1442
AhdI	GACNNNNNGTC	1 cut(s)	2361
AhlI	ACTAGT	1 cut(s)	1808
AjuI	GAANNNNNNNTTGG	4 cut(s)	1930, 1962, 2158, 2190
AleI	CACNNNNGTG	1 cut(s)	119
Alw21I	GWGCWC	3 cut(s)	1125, 1636, 2484
Alw26I	GTCTC	4 cut(s)	104, 912, 1318, 2382
AlwI	GGATC	3 cut(s)	132, 668, 1582
AlwNI	CAGNNNCTG	1 cut(s)	2357
Ama87I	CYCGRG	1 cut(s)	2515
Aor51HI	AGCGCT	1 cut(s)	369
AoxI	GGCC	2 cut(s)	1678, 2184
ApeKI	GCWGC	3 cut(s)	206, 2287, 2338
ApoI	RAATTY	1 cut(s)	63
AseI	ATTAAT	1 cut(s)	530
Asp700I	GAANNNNTTC	1 cut(s)	804
AspLEI	GCGC	3 cut(s)	345, 370, 1788
AspS9I	GGNCC	8 cut(s)	154, 475, 1303, 1445, 1466, 1679, 2184, 2405
AsuC2I	CCSGG	2 cut(s)	2280, 2322
AsuHPI	GGTGA	7 cut(s)	184, 698, 905, 1207, 1601, 1613, 2540
AvaI	CYCGRG	1 cut(s)	2515
AvaII	GGWCC	6 cut(s)	154, 475, 1303, 1445, 1466, 2405
AxyI	CCTNAGG	1 cut(s)	1329
BaeI	ACNNNNGTAYC	2 cut(s)	429, 462
BanI	GGYRCC	2 cut(s)	1763, 2618
BanII	GRGCYC	2 cut(s)	1636, 2609
BarI	GAAGNNNNNNTAC	2 cut(s)	2529, 2561
BbrPI	CACGTG	2 cut(s)	319, 1580
Bbv12I	GWGCWC	3 cut(s)	1125, 1636, 2484
BbvI	GCAGC	3 cut(s)	193, 2299, 2350
BceAI	ACGGC	1 cut(s)	2254
BciVI	GTATCC	1 cut(s)	430
BclI	TGATCA	1 cut(s)	1621
BcnI	CCSGG	2 cut(s)	2280, 2322
BcoDI	GTCTC	4 cut(s)	104, 912, 1318, 2382
BcuI	ACTAGT	1 cut(s)	1808
BfaI	CTAG	9 cut(s)	432, 639, 1614, 1671, 1809, 1911, 2115, 2256, 2720
BfmI	CTRYAG	1 cut(s)	1743
BfoI	RGCGCY	1 cut(s)	371
BfrI	CTTAAG	1 cut(s)	1307
BfuI	GTATCC	1 cut(s)	430
BisI	GCNGC	3 cut(s)	207, 2288, 2339
BlsI	GCNGC	3 cut(s)	208, 2289, 2340
BmcAI	AGTACT	3 cut(s)	766, 1037, 2236
Bme18I	GGWCC	6 cut(s)	154, 475, 1303, 1445, 1466, 2405
BmeRI	GACNNNNNGTC	1 cut(s)	2361
BmeT110I	CYCGRG	1 cut(s)	2515
BmgT120I	GGNCC	8 cut(s)	154, 475, 1303, 1445, 1466, 1679, 2184, 2405
BmiI	GGNNCC	4 cut(s)	156, 476, 1765, 2620
BmrI	ACTGGG	1 cut(s)	1645
BmsI	GCATC	4 cut(s)	13, 886, 1978, 2318
BmuI	ACTGGG	1 cut(s)	1645
BplI	GAGNNNNNCTC	2 cut(s)	2466, 2498
BpmI	CTGGAG	4 cut(s)	590, 2013, 2352, 2469
BpuEI	CTTGAG	2 cut(s)	1062, 1847
BpuMI	CCSGG	2 cut(s)	2280, 2322
BsaAI	YACGTR	3 cut(s)	319, 1280, 1580
BsaBI	GATNNNNATC	3 cut(s)	123, 1512, 2594
BsaHI	GRCGYC	1 cut(s)	1976
BsaI	GGTCTC	3 cut(s)	104, 912, 1318
BsaWI	WCCGGW	1 cut(s)	1857
Bse21I	CCTNAGG	1 cut(s)	1329
Bse3DI	GCAATG	1 cut(s)	2530
Bse8I	GATNNNNATC	3 cut(s)	123, 1512, 2594
BseJI	GATNNNNATC	3 cut(s)	123, 1512, 2594
BseMI	GCAATG	1 cut(s)	2530
BseMII	CTCAG	5 cut(s)	96, 1182, 1311, 1320, 2319
BseRI	GAGGAG	5 cut(s)	122, 1503, 1626, 1629, 1689
BseXI	GCAGC	3 cut(s)	193, 2299, 2350
BseYI	CCCAGC	1 cut(s)	2082
Bsh1236I	CGCG	2 cut(s)	1788, 2616
BshFI	GGCC	2 cut(s)	1680, 2186
BshNI	GGYRCC	2 cut(s)	1763, 2618
BsiHKAI	GWGCWC	3 cut(s)	1125, 1636, 2484
BsiHKCI	CYCGRG	1 cut(s)	2515
BsiSI	CCGG	3 cut(s)	1858, 2280, 2322
BsiWI	CGTACG	1 cut(s)	2154
BslFI	GGGAC	9 cut(s)	488, 976, 1235, 1639, 1753, 1816, 2243, 2368, 2408
BsmAI	GTCTC	4 cut(s)	104, 912, 1318, 2382
BsmFI	GGGAC	9 cut(s)	488, 976, 1235, 1639, 1753, 1816, 2243, 2368, 2408
BsnI	GGCC	2 cut(s)	1680, 2186
Bso31I	GGTCTC	3 cut(s)	104, 912, 1318
BsoBI	CYCGRG	1 cut(s)	2515
Bsp1286I	GDGCHC	4 cut(s)	1125, 1636, 2484, 2609
Bsp143I	GATC	9 cut(s)	124, 673, 1111, 1237, 1356, 1507, 1574, 1621, 2589
Bsp19I	CCATGG	1 cut(s)	2408
BspACI	CCGC	6 cut(s)	302, 611, 940, 1529, 1958, 2616
BspANI	GGCC	2 cut(s)	1680, 2186
BspCNI	CTCAG	5 cut(s)	97, 1183, 1312, 1321, 2318
BspFNI	CGCG	2 cut(s)	1788, 2616
BspHI	TCATGA	2 cut(s)	1234, 2008
BspLI	GGNNCC	4 cut(s)	156, 476, 1765, 2620
BspPI	GGATC	3 cut(s)	132, 668, 1582
BspT107I	GGYRCC	2 cut(s)	1763, 2618
BspTI	CTTAAG	1 cut(s)	1307
BspTNI	GGTCTC	3 cut(s)	104, 912, 1318
BsrBI	CCGCTC	1 cut(s)	1960
BsrDI	GCAATG	1 cut(s)	2530
BssMI	GATC	9 cut(s)	124, 673, 1111, 1237, 1356, 1507, 1574, 1621, 2589
BssNI	GRCGYC	1 cut(s)	1976
BssT1I	CCWWGG	5 cut(s)	483, 1452, 2225, 2408, 2573
Bst6I	CTCTTC	1 cut(s)	1711
BstACI	GRCGYC	1 cut(s)	1976
BstAFI	CTTAAG	1 cut(s)	1307
BstAPI	GCANNNNNTGC	1 cut(s)	77
BstBAI	YACGTR	3 cut(s)	319, 1280, 1580
BstC8I	GCNNGC	6 cut(s)	132, 211, 331, 1527, 1531, 2630
BstDEI	CTNAG	7 cut(s)	105, 1146, 1191, 1320, 1329, 1382, 2305
BstDSI	CCRYGG	2 cut(s)	1780, 2408
BstEII	GGTNACC	1 cut(s)	1775
BstENI	CCTNNNNNAGG	4 cut(s)	482, 1674, 2175, 2385
BstFNI	CGCG	2 cut(s)	1788, 2616
BstH2I	RGCGCY	1 cut(s)	371
BstHHI	GCGC	3 cut(s)	345, 370, 1788
BstKTI	GATC	9 cut(s)	127, 676, 1114, 1240, 1359, 1510, 1577, 1624, 2592
BstMAI	GTCTC	4 cut(s)	104, 912, 1318, 2382
BstMBI	GATC	9 cut(s)	124, 673, 1111, 1237, 1356, 1507, 1574, 1621, 2589
BstMWI	GCNNNNNNNGC	4 cut(s)	77, 1387, 2520, 2613
BstNSI	RCATGY	3 cut(s)	1121, 1299, 1529
BstPI	GGTNACC	1 cut(s)	1775
BstSFI	CTRYAG	1 cut(s)	1743
BstUI	CGCG	2 cut(s)	1788, 2616
BstV1I	GCAGC	3 cut(s)	193, 2299, 2350
BstX2I	RGATCY	1 cut(s)	673
BstXI	CCANNNNNNTGG	2 cut(s)	121, 165
BstYI	RGATCY	1 cut(s)	673
Bsu36I	CCTNAGG	1 cut(s)	1329
BsuI	GTATCC	1 cut(s)	430
BsuRI	GGCC	2 cut(s)	1680, 2186
BtgI	CCRYGG	2 cut(s)	1780, 2408
BtsIMutI	CAGTG	2 cut(s)	114, 2622
Cac8I	GCNNGC	6 cut(s)	132, 211, 331, 1527, 1531, 2630
CaiI	CAGNNNCTG	1 cut(s)	2357
CciI	TCATGA	2 cut(s)	1234, 2008
CfoI	GCGC	3 cut(s)	345, 370, 1788
Cfr13I	GGNCC	8 cut(s)	154, 475, 1303, 1445, 1466, 1679, 2184, 2405
CseI	GACGC	1 cut(s)	1984
DdeI	CTNAG	7 cut(s)	105, 1146, 1191, 1320, 1329, 1382, 2305
DpnI	GATC	9 cut(s)	126, 675, 1113, 1239, 1358, 1509, 1576, 1623, 2591
DpnII	GATC	9 cut(s)	124, 673, 1111, 1237, 1356, 1507, 1574, 1621, 2589
DraI	TTTAAA	1 cut(s)	721
DraIII	CACNNNGTG	1 cut(s)	886
DriI	GACNNNNNGTC	1 cut(s)	2361
Eam1104I	CTCTTC	1 cut(s)	1711
Eam1105I	GACNNNNNGTC	1 cut(s)	2361
EarI	CTCTTC	1 cut(s)	1711
EciI	GGCGGA	1 cut(s)	600
Ecl136II	GAGCTC	1 cut(s)	1634
Eco130I	CCWWGG	5 cut(s)	483, 1452, 2225, 2408, 2573
Eco24I	GRGCYC	2 cut(s)	1636, 2609
Eco31I	GGTCTC	3 cut(s)	104, 912, 1318
Eco47I	GGWCC	6 cut(s)	154, 475, 1303, 1445, 1466, 2405
Eco47III	AGCGCT	1 cut(s)	369
Eco53kI	GAGCTC	1 cut(s)	1634
Eco57I	CTGAAG	2 cut(s)	299, 1311
Eco72I	CACGTG	2 cut(s)	319, 1580
Eco81I	CCTNAGG	1 cut(s)	1329
Eco88I	CYCGRG	1 cut(s)	2515
Eco91I	GGTNACC	1 cut(s)	1775
EcoICRI	GAGCTC	1 cut(s)	1634
EcoNI	CCTNNNNNAGG	4 cut(s)	482, 1674, 2175, 2385
EcoO109I	RGGNCCY	5 cut(s)	475, 1303, 1445, 1466, 1679
EcoO65I	GGTNACC	1 cut(s)	1775
EcoRI	GAATTC	1 cut(s)	63
EcoT14I	CCWWGG	5 cut(s)	483, 1452, 2225, 2408, 2573
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	2 cut(s)	1636, 2609
ErhI	CCWWGG	5 cut(s)	483, 1452, 2225, 2408, 2573
FalI	AAGNNNNNCTT	2 cut(s)	462, 494
FaqI	GGGAC	9 cut(s)	488, 976, 1235, 1639, 1753, 1816, 2243, 2368, 2408
FauI	CCCGC	2 cut(s)	933, 1522
FbaI	TGATCA	1 cut(s)	1621
Fnu4HI	GCNGC	3 cut(s)	207, 2288, 2339
FriOI	GRGCYC	2 cut(s)	1636, 2609
Fsp4HI	GCNGC	3 cut(s)	207, 2288, 2339
FspBI	CTAG	9 cut(s)	432, 639, 1614, 1671, 1809, 1911, 2115, 2256, 2720
FspI	TGCGCA	1 cut(s)	344
GlaI	GCGC	3 cut(s)	344, 369, 1787
GluI	GCNGC	3 cut(s)	207, 2288, 2339
GsaI	CCCAGC	1 cut(s)	2086
GsuI	CTGGAG	4 cut(s)	590, 2013, 2352, 2469
HaeII	RGCGCY	1 cut(s)	371
HaeIII	GGCC	2 cut(s)	1680, 2186
HapII	CCGG	3 cut(s)	1858, 2280, 2322
HgaI	GACGC	1 cut(s)	1984
HhaI	GCGC	3 cut(s)	345, 370, 1788
Hin1I	GRCGYC	1 cut(s)	1976
Hin6I	GCGC	3 cut(s)	343, 368, 1786
HinP1I	GCGC	3 cut(s)	343, 368, 1786
HincII	GTYRAC	3 cut(s)	852, 1855, 2274
HindII	GTYRAC	3 cut(s)	852, 1855, 2274
HindIII	AAGCTT	1 cut(s)	1379
HinfI	GANTC	8 cut(s)	1051, 1491, 1604, 1753, 1829, 2023, 2047, 2065
HpaII	CCGG	3 cut(s)	1858, 2280, 2322
HphI	GGTGA	7 cut(s)	184, 698, 905, 1207, 1601, 1613, 2540
Hpy166II	GTNNAC	6 cut(s)	668, 852, 1460, 1814, 1855, 2274
Hpy188I	TCNGA	8 cut(s)	449, 810, 977, 1138, 1192, 1873, 1932, 2474
Hpy8I	GTNNAC	6 cut(s)	668, 852, 1460, 1814, 1855, 2274
HpyCH4IV	ACGT	7 cut(s)	294, 318, 616, 1279, 1579, 1878, 2153
HpyF10VI	GCNNNNNNNGC	4 cut(s)	77, 1387, 2520, 2613
HpyF3I	CTNAG	7 cut(s)	105, 1146, 1191, 1320, 1329, 1382, 2305
HpySE526I	ACGT	7 cut(s)	294, 318, 616, 1279, 1579, 1878, 2153
Hsp92I	GRCGYC	1 cut(s)	1976
HspAI	GCGC	3 cut(s)	343, 368, 1786
Ksp22I	TGATCA	1 cut(s)	1621
Kzo9I	GATC	9 cut(s)	124, 673, 1111, 1237, 1356, 1507, 1574, 1621, 2589
LmnI	GCTCC	7 cut(s)	661, 1639, 1965, 1994, 2167, 2257, 2487
Lsp1109I	GCAGC	3 cut(s)	193, 2299, 2350
LweI	GCATC	4 cut(s)	13, 886, 1978, 2318
MaeI	CTAG	9 cut(s)	432, 639, 1614, 1671, 1809, 1911, 2115, 2256, 2720
MaeII	ACGT	7 cut(s)	294, 318, 616, 1279, 1579, 1878, 2153
MaeIII	GTNAC	9 cut(s)	517, 686, 1195, 1548, 1601, 1775, 1846, 2033, 2536
MalI	GATC	9 cut(s)	126, 675, 1113, 1239, 1358, 1509, 1576, 1623, 2591
MbiI	CCGCTC	1 cut(s)	1960
MboI	GATC	9 cut(s)	124, 673, 1111, 1237, 1356, 1507, 1574, 1621, 2589
MflI	RGATCY	1 cut(s)	673
MhlI	GDGCHC	4 cut(s)	1125, 1636, 2484, 2609
MlyI	GAGTC	5 cut(s)	1500, 1598, 1838, 2017, 2074
MmeI	TCCRAC	1 cut(s)	2189
Mph1103I	ATGCAT	1 cut(s)	6
MroXI	GAANNNNTTC	1 cut(s)	804
MseI	TTAA	9 cut(s)	215, 530, 720, 1308, 1349, 1401, 1545, 1800, 2688
MslI	CAYNNNNRTG	3 cut(s)	119, 654, 1158
MspCI	CTTAAG	1 cut(s)	1307
MspI	CCGG	3 cut(s)	1858, 2280, 2322
MvnI	CGCG	2 cut(s)	1788, 2616
MwoI	GCNNNNNNNGC	4 cut(s)	77, 1387, 2520, 2613
NciI	CCSGG	2 cut(s)	2280, 2322
NcoI	CCATGG	1 cut(s)	2408
NdeII	GATC	9 cut(s)	124, 673, 1111, 1237, 1356, 1507, 1574, 1621, 2589
NlaIV	GGNNCC	4 cut(s)	156, 476, 1765, 2620
NmuCI	GTSAC	6 cut(s)	686, 1195, 1548, 1601, 1846, 2033
NsbI	TGCGCA	1 cut(s)	344
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	3 cut(s)	1121, 1299, 1529
OliI	CACNNNNGTG	1 cut(s)	119
PaeI	GCATGC	1 cut(s)	1529
PagI	TCATGA	2 cut(s)	1234, 2008
PcsI	WCGNNNNNNNCGW	2 cut(s)	661, 2565
PdmI	GAANNNNTTC	1 cut(s)	804
PfeI	GAWTC	3 cut(s)	1051, 1753, 2047
Pfl23II	CGTACG	1 cut(s)	2154
PflMI	CCANNNNNTGG	2 cut(s)	2171, 2357
PfoI	TCCNGGA	3 cut(s)	2320, 2329, 2484
PkrI	GCNGC	3 cut(s)	208, 2289, 2340
PleI	GAGTC	5 cut(s)	1499, 1598, 1837, 2017, 2073
PmaCI	CACGTG	2 cut(s)	319, 1580
PmlI	CACGTG	2 cut(s)	319, 1580
PpsI	GAGTC	5 cut(s)	1499, 1598, 1837, 2017, 2073
Ppu21I	YACGTR	3 cut(s)	319, 1280, 1580
PpuMI	RGGWCCY	4 cut(s)	475, 1303, 1445, 1466
PshBI	ATTAAT	1 cut(s)	530
Psp124BI	GAGCTC	1 cut(s)	1636
Psp5II	RGGWCCY	4 cut(s)	475, 1303, 1445, 1466
PspCI	CACGTG	2 cut(s)	319, 1580
PspEI	GGTNACC	1 cut(s)	1775
PspFI	CCCAGC	1 cut(s)	2082
PspLI	CGTACG	1 cut(s)	2154
PspN4I	GGNNCC	4 cut(s)	156, 476, 1765, 2620
PspPI	GGNCC	8 cut(s)	154, 475, 1303, 1445, 1466, 1679, 2184, 2405
PspPPI	RGGWCCY	4 cut(s)	475, 1303, 1445, 1466
PsrI	GAACNNNNNNTAC	2 cut(s)	275, 307
PstNI	CAGNNNCTG	1 cut(s)	2357
PsuI	RGATCY	1 cut(s)	673
RseI	CAYNNNNRTG	3 cut(s)	119, 654, 1158
SacI	GAGCTC	1 cut(s)	1636
SaqAI	TTAA	9 cut(s)	215, 530, 720, 1308, 1349, 1401, 1545, 1800, 2688
SatI	GCNGC	3 cut(s)	207, 2288, 2339
Sau3AI	GATC	9 cut(s)	124, 673, 1111, 1237, 1356, 1507, 1574, 1621, 2589
Sau96I	GGNCC	8 cut(s)	154, 475, 1303, 1445, 1466, 1679, 2184, 2405
ScaI	AGTACT	3 cut(s)	766, 1037, 2236
SchI	GAGTC	5 cut(s)	1500, 1598, 1838, 2017, 2074
SduI	GDGCHC	4 cut(s)	1125, 1636, 2484, 2609
SfaNI	GCATC	4 cut(s)	13, 886, 1978, 2318
SfcI	CTRYAG	1 cut(s)	1743
SinI	GGWCC	6 cut(s)	154, 475, 1303, 1445, 1466, 2405
SmiMI	CAYNNNNRTG	3 cut(s)	119, 654, 1158
SmlI	CTYRAG	3 cut(s)	1077, 1307, 1826
SmoI	CTYRAG	3 cut(s)	1077, 1307, 1826
SpeI	ACTAGT	1 cut(s)	1808
SphI	GCATGC	1 cut(s)	1529
SsiI	CCGC	6 cut(s)	302, 611, 940, 1529, 1958, 2616
SspI	AATATT	2 cut(s)	628, 2694
SspMI	CTAG	9 cut(s)	432, 639, 1614, 1671, 1809, 1911, 2115, 2256, 2720
SstI	GAGCTC	1 cut(s)	1636
StyI	CCWWGG	5 cut(s)	483, 1452, 2225, 2408, 2573
TaiI	ACGT	7 cut(s)	297, 321, 619, 1282, 1582, 1881, 2156
TaqI	TCGA	6 cut(s)	45, 1110, 1630, 1951, 2559, 2643
TaqII	GACCGA	1 cut(s)	2052
TatI	WGTACW	4 cut(s)	764, 1035, 2234, 2542
TfiI	GAWTC	3 cut(s)	1051, 1753, 2047
Tru1I	TTAA	9 cut(s)	215, 530, 720, 1308, 1349, 1401, 1545, 1800, 2688
Tru9I	TTAA	9 cut(s)	215, 530, 720, 1308, 1349, 1401, 1545, 1800, 2688
TscAI	CASTG	2 cut(s)	121, 2629
TseFI	GTSAC	6 cut(s)	686, 1195, 1548, 1601, 1846, 2033
TseI	GCWGC	3 cut(s)	206, 2287, 2338
Tsp45I	GTSAC	6 cut(s)	686, 1195, 1548, 1601, 1846, 2033
TspGWI	ACGGA	5 cut(s)	394, 1148, 2040, 2164, 2666
TspRI	CASTG	2 cut(s)	121, 2629
Van91I	CCANNNNNTGG	2 cut(s)	2171, 2357
Vha464I	CTTAAG	1 cut(s)	1307
VpaK11BI	GGWCC	6 cut(s)	154, 475, 1303, 1445, 1466, 2405
VspI	ATTAAT	1 cut(s)	530
XagI	CCTNNNNNAGG	4 cut(s)	482, 1674, 2175, 2385
XapI	RAATTY	1 cut(s)	63
XbaI	TCTAGA	1 cut(s)	1613
XceI	RCATGY	3 cut(s)	1121, 1299, 1529
XmnI	GAANNNNTTC	1 cut(s)	804
XspI	CTAG	9 cut(s)	432, 639, 1614, 1671, 1809, 1911, 2115, 2256, 2720
ZrmI	AGTACT	3 cut(s)	766, 1037, 2236
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.