Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Forward (+)

1840285 .. 1849236

Gene Structure 8952 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1013600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3135 bp

ATGGCTCGTCACAGAGACGAATATGACGACGAGATGGAGGAAGAGGAAGAATACGACCCGGACCAAGCTATAGACGAGGATGACGAGGAGGAAGAGGAGGAGGAACGAGGCGGAGGAAGTCGGCGGCCCAGTCAGAAGCGGAGGAGATCGGATTTCATAGACGATGTAGCAGAGGAGGACGATGACGAGGAGGAAGAGGAGTACGGTGAGGATGACGAGGCTTATGGTGGTGGTGGTGGTGCGAGTCGTAAGAGGAAGAACAAGAGGCCCTCTGGGTCGCAGTTTCTCGACATTGAGGCCGAGGTCGATACCGACGAAGAAGAAGAAGACGAGGAGGGGGATGATGACTTTATTGCTGATAATGCACCTGATCTACCTGAAGAAGAGGATGGTAGAAGGATGCGTCATCGACCGTTGATGCAAGAAGATGAGCAAGAAGATCTTGAAGAGATTGAAAGAAATCTTCAAGAGCGGTATGGGAAGTCAAGTCATCGAGAGTATGATGAAGACACAACTGATGTGGATCAACAAGCTCTTTTACCTTCTGTTAGGGACCCAAAGTTGTGGATGGTGAAATGTGCGATTGGTCGTGAGCGGGAGGTAGCTGTTTGCTTAATGCAAAAGTATATCGATAAACCTGAACTGCAGATCAGGTCTGTTATTGCTCTTGACCACCTTAAAAACTATGTATACGTTGAAGCGGACAAAGAAGCCCATGTGAGGGAGGCTTGCAAAGGTCTGCGCAATATATTTGGCCAGAAAATAATGCTTGTCCCAATTAGAGAAATGACTGATGTTCTTTCAGTTGAAAGCAAGGCTATTGATCTTTCTAGGGATACGTGGGTCAGAATGAAGAGTGGGACATATAAAGGAGACCTTGCCCAGGTTGCAGATGTGGACAATGTGCGGCAGAGAGTTACAGTTAAATTAATTCCAAGGATTGATTTACAAGCTCTTGCAAATAAAATGGATGGCAGAGAAGTTGTGAGAACAAAAGCATTTGTTCCTCCTCCACGCTTATTAAATATTGAGGAAGCAAGGGAACTGCATATTCTTGGAGATCGTAAACAAGATCGGATGACTGGTGATTATTATCAGACGATTAATGGCATGATGTTTAAAGATGGTTTCCTGTATAAAGCAGTATCAATCAAATCAATTAGTACTCAGAACATACATCCCACGTTTGACGAACTTGAAAAATTTCGGAAGCCCAGCGAGAATGGTGATGGAGATATTGCAAGTCTGTCAACTTTATTTTCAAACAGAAAGAAGGGACACTTTCTGAAGGGCGATGCAGTTATTGTTGTCAAGGGAGATCTAAAAAATTTGAAGGGATGGGTTGAGAAAGTTGAGGAGGATACTGTTCATATCAGACCTGAAATCAAAGAACTCCCAAAAACCCTTGCCATCAATGAAAAAGAGCTGTGCAAATACTTTGAACCTGGGAATCATGTGAAAGTTGTATCTGGCACTCAGGAAGGTGCAACTGGTATGGTTGTTAAGGTTGAGCAGCATGTTCTCATCATCCTATCTGATGTAACGAAAGAACATATCCGTGTGTTTGCTGATGATGTTGTGGAGAGCTCTGAGGTGACATCTGGTATTACCAGAATTGGGGCATATGAGCTTCATGATCTCGTGCTACTGGATAATAATAGCTTCGGTGTAATCATACGCGTAGAAAGTGAAGCATTCCAGGTTCTTAAGGGAGTTCCTGATAGACCTGAGGTTGCACTTGTCAAGTTAAGAGAGATCAAATGCAAGATTGAGAAAAGTTTTCCTGTGGAAGTTAAGTATAAACACAAAGTATCCGTGAAGGATGTTGTGAGGGTCATTGATGGCCCTTGCGAGGGGAAACAGGGTCCTGTGCAACACATATACAGGGGAGTCTTGTTCATCTTTGATCGCCACCACCTGGAGCATGCAGGCTTTATCTGTGTTAAGTCTCATAATTGTGCTCTTGTGGGAGGTTCACGTGCTAATGGTGATAGAAATGGTAACTCCAATTCACGATATGACCACCTCAGAACTCCTCCTCGAGTTCCTCAGTCCCCGAATAGGTTTTCTAGAGGAGGCCCTCCATCTAACTATGGAGGAAGGAATAGAGGTGGACGAGGGCATGATGGCTTGGTTGGGACGACAGTAAAGATTCGCCAGGGTGCCTATAAGGGCTATCGTGGACGTGTTGTTGAGGTTAAGGGACCAAATGTACGGGTTGAGTTGGAGTCTCAAATGAAGGTTGTTACAGTTGATCGTAATTGTATTTCTGATAATGTGACTGTTACCACACCATATCGTGATACATCTCGCTACGGTATGGGAAGTGAGACTCCTATGCATCCTTCTCGAACTCCACTCCATCCATATATGACTCCTATGAGGGATGCTGCAGCAACACCTATACATGATGGCATGAGGACTCCTATGCGTGACCGAGCATGGAATCCCTATGCACCTATGAGTCCAGCTAGGGATAACTGGGAGGAAGGAAACCCTGCTTCTTGGGGTGCTAGCCCACAGTATCAGCCAGGAAGTCCTCCTTCGCGGGCATATGAAGCACCAACTCCTGGTTCAGGTTGGGCAAACACTCCTGGTGGAAATTATAGTGAAGCTGGTACACCGAGGGACAGTAGTTCCGCTTATGCAAATGCTCCTAGCCCTTACTTGCCATCGACTCCAGGTGGCCAGCCTATGACACCAAATTCGGCATCCTACCTTCCTGGAACCCCTGGAGGGCAGCCAATGACACCAGGCACTGGTGGTCTGGATATGTTGTCTCCTGTTATAGGTGGGGACAGTGAAGGACCCTGGTTCCTCCCAGACATATTGGTCAATGTCCACAATTCTGGAGAGGAAACTACTGGAGTTGTGAGAGAAGTGCTTCCGGATGGCTCCTATAGGGTAGCTATTGGGTCGGGTGGCAACGGGGAAACCATAATAGCACTTCCTAATGAAATGGAGGCAGTGGTACCGAAGAAAAGTGATAAGATAAAGATCATGGGTGGGTCACTACGAGGTGCGACCGGTAAGCTGATTGGTGTTGATGGCACAGACGGAATTGTAAAGGTAGATGACACTCTTGATGTTAAGATTTTAGACTTGGCTATTTTGTCAAAACTAGCCCAATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

1045

Amino Acids

116.12

Weight (kDa)

5.15

Isoelectric Point (pI)

45.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5_N	PF11942	95 - 181	9e-17	Spt5 transcription elongation factor, acidic N-terminal
Spt5-NGN	PF03439	187 - 267	1.4e-26	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	273 - 402	4.7e-68	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	430 - 479	2e-22	Transcription elongation factor SPT5, second KOW domain
KOWx_SPT5	PF23037	538 - 589	1.6e-17	Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5	PF23291	600 - 650	5.2e-21	Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5	PF23290	711 - 754	1.7e-17	Transcription elongation factor SPT5, fifth KOW domain
KOW6_SPT51-2	PF23038	939 - 988	2e-25	Transcription elongation factor SPT5 homolog 1/2, KOW6 domain
KOW7_SPT5	PF23287	990 - 1041	8.7e-21	Transcription elongation factor SPT5, seventh KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2831
Acc16I	TGCGCA	1 cut(s)	743
Acc65I	GGTACC	1 cut(s)	2971
AccB1I	GGYRCC	2 cut(s)	2162, 2971
AccB7I	CCANNNNNTGG	3 cut(s)	1918, 2570, 2759
AccBSI	CCGCTC	2 cut(s)	472, 595
AccI	GTMKAC	1 cut(s)	690
AccII	CGCG	2 cut(s)	1680, 2548
AccIII	TCCGGA	1 cut(s)	2887
AciI	CCGC	9 cut(s)	111, 124, 139, 472, 595, 701, 907, 2548, 2640
AclWI	GGATC	1 cut(s)	531
AcoI	YGGCCR	2 cut(s)	754, 2686
AcsI	RAATTY	3 cut(s)	1202, 1327, 2704
AcuI	CTGAAG	2 cut(s)	399, 1307
AcvI	CACGTG	1 cut(s)	1979
AfaI	GTAC	5 cut(s)	203, 1165, 2214, 2620, 2973
AflII	CTTAAG	1 cut(s)	1706
AflIII	ACRYGT	2 cut(s)	1678, 2185
AgeI	ACCGGT	1 cut(s)	3026
AgsI	TTSAA	9 cut(s)	446, 455, 467, 698, 809, 1199, 1263, 1333, 1442
AjiI	CACGTC	1 cut(s)	2186
AjuI	GAANNNNNNNTTGG	2 cut(s)	2557, 2589
Alw21I	GWGCWC	2 cut(s)	1589, 1963
Alw26I	GTCTC	6 cut(s)	9, 867, 1953, 2235, 2324, 2784
AlwI	GGATC	1 cut(s)	531
AlwNI	CAGNNNCTG	1 cut(s)	2759
Ama87I	CYCGRG	1 cut(s)	2040
Aor13HI	TCCGGA	1 cut(s)	2887
AoxI	GGCC	7 cut(s)	125, 266, 297, 754, 1843, 2077, 2686
ApeKI	GCWGC	4 cut(s)	1513, 2390, 2393, 2740
ApoI	RAATTY	3 cut(s)	1202, 1327, 2704
ArsI	GACNNNNNNTTYG	2 cut(s)	2689, 2721
AseI	ATTAAT	2 cut(s)	929, 1104
AsiGI	ACCGGT	1 cut(s)	3026
Asp700I	GAANNNNTTC	2 cut(s)	1203, 2883
Asp718I	GGTACC	1 cut(s)	2971
AspLEI	GCGC	1 cut(s)	744
AspS9I	GGNCC	9 cut(s)	61, 126, 267, 553, 1844, 1865, 2078, 2204, 2807
AsuC2I	CCSGG	1 cut(s)	59
AsuHPI	GGTGA	6 cut(s)	218, 583, 1097, 1238, 1606, 2000
AsuNHI	GCTAGC	1 cut(s)	2513
AvaI	CYCGRG	1 cut(s)	2040
AvaII	GGWCC	5 cut(s)	61, 553, 1865, 2204, 2807
AxyI	CCTNAGG	1 cut(s)	1728
BaeI	ACNNNNGTAYC	2 cut(s)	828, 861
BalI	TGGCCA	2 cut(s)	756, 2688
BanI	GGYRCC	2 cut(s)	2162, 2971
BanII	GRGCYC	1 cut(s)	1589
BauI	CACGAG	1 cut(s)	1640
BbrPI	CACGTG	1 cut(s)	1979
BbsI	GAAGAC	2 cut(s)	333, 513
Bbv12I	GWGCWC	2 cut(s)	1589, 1963
BbvI	GCAGC	4 cut(s)	1525, 2377, 2405, 2752
BcgI	CGANNNNNNTGC	2 cut(s)	2330, 2364
BciVI	GTATCC	3 cut(s)	829, 1354, 1822
BcnI	CCSGG	1 cut(s)	59
BcoDI	GTCTC	6 cut(s)	9, 867, 1953, 2235, 2324, 2784
BfaI	CTAG	6 cut(s)	831, 2070, 2472, 2514, 2658, 3122
BfmI	CTRYAG	4 cut(s)	69, 644, 2391, 2899
BfrI	CTTAAG	1 cut(s)	1706
BfuI	GTATCC	3 cut(s)	829, 1354, 1822
BglII	AGATCT	2 cut(s)	439, 1318
BisI	GCNGC	6 cut(s)	125, 908, 1514, 2391, 2394, 2741
BlsI	GCNGC	6 cut(s)	126, 909, 1515, 2392, 2395, 2742
BmcAI	AGTACT	1 cut(s)	1165
Bme18I	GGWCC	5 cut(s)	61, 553, 1865, 2204, 2807
BmeT110I	CYCGRG	1 cut(s)	2040
BmgBI	CACGTC	1 cut(s)	2186
BmgT120I	GGNCC	9 cut(s)	61, 126, 267, 553, 1844, 1865, 2078, 2204, 2807
BmrI	ACTGGG	2 cut(s)	123, 2491
BmsI	GCATC	6 cut(s)	390, 408, 1285, 2350, 2377, 2720
BmtI	GCTAGC	1 cut(s)	2517
BmuI	ACTGGG	2 cut(s)	123, 2491
BpiI	GAAGAC	2 cut(s)	333, 513
BpmI	CTGGAG	5 cut(s)	1940, 2664, 2754, 2871, 2886
BpuMI	CCSGG	1 cut(s)	59
Bsa29I	ATCGAT	1 cut(s)	630
BsaAI	YACGTR	2 cut(s)	840, 1979
BsaBI	GATNNNNATC	3 cut(s)	522, 1092, 2996
BsaI	GGTCTC	1 cut(s)	867
BsaJI	CCNNGG	8 cut(s)	300, 882, 935, 1445, 2158, 2624, 2731, 2810
BsaWI	WCCGGW	2 cut(s)	2887, 3026
Bse118I	RCCGGY	1 cut(s)	3026
Bse1I	ACTGG	7 cut(s)	129, 1087, 1495, 1653, 2486, 2764, 2869
Bse21I	CCTNAGG	1 cut(s)	1728
Bse8I	GATNNNNATC	3 cut(s)	522, 1092, 2996
BseAI	TCCGGA	1 cut(s)	2887
BseCI	ATCGAT	1 cut(s)	630
BseDI	CCNNGG	8 cut(s)	300, 882, 935, 1445, 2158, 2624, 2731, 2810
BseJI	GATNNNNATC	3 cut(s)	522, 1092, 2996
BseMII	CTCAG	6 cut(s)	1181, 1490, 1581, 1719, 2041, 2063
BseNI	ACTGG	7 cut(s)	129, 1087, 1495, 1653, 2486, 2764, 2869
BseXI	GCAGC	4 cut(s)	1525, 2377, 2405, 2752
BseYI	CCCAGC	1 cut(s)	1214
Bsh1236I	CGCG	2 cut(s)	1680, 2548
Bsh1285I	CGRYCG	2 cut(s)	413, 3027
BshFI	GGCC	7 cut(s)	127, 268, 299, 756, 1845, 2079, 2688
BshNI	GGYRCC	2 cut(s)	2162, 2971
BshTI	ACCGGT	1 cut(s)	3026
BshVI	ATCGAT	1 cut(s)	630
BsiEI	CGRYCG	2 cut(s)	413, 3027
BsiHKAI	GWGCWC	2 cut(s)	1589, 1963
BsiHKCI	CYCGRG	1 cut(s)	2040
BsiSI	CCGG	3 cut(s)	59, 2888, 3027
BslFI	GGGAC	9 cut(s)	566, 758, 874, 1290, 2038, 2152, 2217, 2642, 2810
BsmAI	GTCTC	6 cut(s)	9, 867, 1953, 2235, 2324, 2784
BsmBI	CGTCTC	1 cut(s)	9
BsmFI	GGGAC	9 cut(s)	566, 758, 874, 1290, 2038, 2152, 2217, 2642, 2810
BsmI	GAATGC	1 cut(s)	1694
BsnI	GGCC	7 cut(s)	127, 268, 299, 756, 1845, 2079, 2688
Bso31I	GGTCTC	1 cut(s)	867
BsoBI	CYCGRG	1 cut(s)	2040
Bsp1286I	GDGCHC	2 cut(s)	1589, 1963
Bsp13I	TCCGGA	1 cut(s)	2887
BspACI	CCGC	9 cut(s)	111, 124, 139, 472, 595, 701, 907, 2548, 2640
BspANI	GGCC	7 cut(s)	127, 268, 299, 756, 1845, 2079, 2688
BspCNI	CTCAG	6 cut(s)	1180, 1489, 1582, 1720, 2040, 2062
BspDI	ATCGAT	1 cut(s)	630
BspEI	TCCGGA	1 cut(s)	2887
BspFNI	CGCG	2 cut(s)	1680, 2548
BspHI	TCATGA	1 cut(s)	1633
BspMAI	CTGCAG	2 cut(s)	648, 2395
BspOI	GCTAGC	1 cut(s)	2517
BspPI	GGATC	1 cut(s)	531
BspT107I	GGYRCC	2 cut(s)	2162, 2971
BspTI	CTTAAG	1 cut(s)	1706
BspTNI	GGTCTC	1 cut(s)	867
BsrBI	CCGCTC	2 cut(s)	472, 595
BsrFI	RCCGGY	1 cut(s)	3026
BsrI	ACTGG	7 cut(s)	129, 1087, 1495, 1653, 2486, 2764, 2869
BssAI	RCCGGY	1 cut(s)	3026
BssECI	CCNNGG	8 cut(s)	300, 882, 935, 1445, 2158, 2624, 2731, 2810
BssNAI	GTATAC	1 cut(s)	691
BssSI	CACGAG	1 cut(s)	1640
BssT1I	CCWWGG	1 cut(s)	935
Bst1107I	GTATAC	1 cut(s)	691
Bst2BI	CACGAG	1 cut(s)	1640
Bst6I	CTCTTC	6 cut(s)	36, 87, 189, 378, 441, 848
BstAFI	CTTAAG	1 cut(s)	1706
BstBAI	YACGTR	2 cut(s)	840, 1979
BstC8I	GCNNGC	6 cut(s)	730, 1926, 1930, 2515, 2550, 2690
BstDEI	CTNAG	6 cut(s)	1167, 1476, 1590, 1728, 2027, 2049
BstENI	CCTNNNNNAGG	3 cut(s)	881, 2574, 2787
BstFNI	CGCG	2 cut(s)	1680, 2548
BstHHI	GCGC	1 cut(s)	744
BstMAI	GTCTC	6 cut(s)	9, 867, 1953, 2235, 2324, 2784
BstMCI	CGRYCG	2 cut(s)	413, 3027
BstMWI	GCNNNNNNNGC	3 cut(s)	362, 887, 2558
BstNSI	RCATGY	2 cut(s)	1520, 1928
BstSFI	CTRYAG	4 cut(s)	69, 644, 2391, 2899
BstUI	CGCG	2 cut(s)	1680, 2548
BstV1I	GCAGC	4 cut(s)	1525, 2377, 2405, 2752
BstV2I	GAAGAC	2 cut(s)	333, 513
BstX2I	RGATCY	2 cut(s)	439, 1318
BstXI	CCANNNNNNTGG	2 cut(s)	564, 2849
BstYI	RGATCY	2 cut(s)	439, 1318
BstZ17I	GTATAC	1 cut(s)	691
Bsu15I	ATCGAT	1 cut(s)	630
Bsu36I	CCTNAGG	1 cut(s)	1728
BsuI	GTATCC	3 cut(s)	829, 1354, 1822
BsuRI	GGCC	7 cut(s)	127, 268, 299, 756, 1845, 2079, 2688
BsuTUI	ATCGAT	1 cut(s)	630
BtgZI	GCGATG	1 cut(s)	1308
BtrI	CACGTC	1 cut(s)	2186
BtsI	GCAGTG	1 cut(s)	2973
BtsIMutI	CAGTG	3 cut(s)	2757, 2806, 2973
Cac8I	GCNNGC	6 cut(s)	730, 1926, 1930, 2515, 2550, 2690
CaiI	CAGNNNCTG	1 cut(s)	2759
CciI	TCATGA	1 cut(s)	1633
CfoI	GCGC	1 cut(s)	744
Cfr10I	RCCGGY	1 cut(s)	3026
Cfr13I	GGNCC	9 cut(s)	61, 126, 267, 553, 1844, 1865, 2078, 2204, 2807
ClaI	ATCGAT	1 cut(s)	630
CseI	GACGC	1 cut(s)	392
Csp6I	GTAC	5 cut(s)	202, 1164, 2213, 2619, 2972
CspAI	ACCGGT	1 cut(s)	3026
CspCI	CAANNNNNGTGG	2 cut(s)	501, 536
CviQI	GTAC	5 cut(s)	202, 1164, 2213, 2619, 2972
DdeI	CTNAG	6 cut(s)	1167, 1476, 1590, 1728, 2027, 2049
DraI	TTTAAA	1 cut(s)	1120
DrdI	GACNNNNNNGTC	1 cut(s)	2831
DseDI	GACNNNNNNGTC	1 cut(s)	2831
EaeI	YGGCCR	2 cut(s)	754, 2686
Eam1104I	CTCTTC	6 cut(s)	36, 87, 189, 378, 441, 848
EarI	CTCTTC	6 cut(s)	36, 87, 189, 378, 441, 848
EciI	GGCGGA	1 cut(s)	126
Ecl136II	GAGCTC	1 cut(s)	1587
Eco130I	CCWWGG	1 cut(s)	935
Eco24I	GRGCYC	1 cut(s)	1589
Eco31I	GGTCTC	1 cut(s)	867
Eco47I	GGWCC	5 cut(s)	61, 553, 1865, 2204, 2807
Eco53kI	GAGCTC	1 cut(s)	1587
Eco57I	CTGAAG	2 cut(s)	399, 1307
Eco72I	CACGTG	1 cut(s)	1979
Eco81I	CCTNAGG	1 cut(s)	1728
Eco88I	CYCGRG	1 cut(s)	2040
EcoICRI	GAGCTC	1 cut(s)	1587
EcoNI	CCTNNNNNAGG	3 cut(s)	881, 2574, 2787
EcoO109I	RGGNCCY	5 cut(s)	267, 553, 1865, 2078, 2807
EcoT14I	CCWWGG	1 cut(s)	935
EcoT22I	ATGCAT	1 cut(s)	2343
EcoT38I	GRGCYC	1 cut(s)	1589
ErhI	CCWWGG	1 cut(s)	935
Esp3I	CGTCTC	1 cut(s)	9
FalI	AAGNNNNNCTT	8 cut(s)	426, 458, 861, 893, 1265, 1297, 2527, 2559
FaqI	GGGAC	9 cut(s)	566, 758, 874, 1290, 2038, 2152, 2217, 2642, 2810
FauI	CCCGC	2 cut(s)	588, 2541
FauNDI	CATATG	2 cut(s)	1624, 2554
FblI	GTMKAC	1 cut(s)	690
Fnu4HI	GCNGC	6 cut(s)	125, 908, 1514, 2391, 2394, 2741
FriOI	GRGCYC	1 cut(s)	1589
Fsp4HI	GCNGC	6 cut(s)	125, 908, 1514, 2391, 2394, 2741
FspBI	CTAG	6 cut(s)	831, 2070, 2472, 2514, 2658, 3122
FspI	TGCGCA	1 cut(s)	743
GlaI	GCGC	1 cut(s)	743
GluI	GCNGC	6 cut(s)	125, 908, 1514, 2391, 2394, 2741
GsaI	CCCAGC	1 cut(s)	1218
GsuI	CTGGAG	5 cut(s)	1940, 2664, 2754, 2871, 2886
HaeIII	GGCC	7 cut(s)	127, 268, 299, 756, 1845, 2079, 2688
HapII	CCGG	3 cut(s)	59, 2888, 3027
HgaI	GACGC	1 cut(s)	392
HhaI	GCGC	1 cut(s)	744
Hin6I	GCGC	1 cut(s)	742
HinP1I	GCGC	1 cut(s)	742
HincII	GTYRAC	1 cut(s)	1251
HindII	GTYRAC	1 cut(s)	1251
HpaII	CCGG	3 cut(s)	59, 2888, 3027
HphI	GGTGA	6 cut(s)	218, 583, 1097, 1238, 1606, 2000
Hpy166II	GTNNAC	9 cut(s)	691, 898, 1067, 1251, 1976, 2114, 2183, 2621, 2842
Hpy8I	GTNNAC	9 cut(s)	691, 898, 1067, 1251, 1976, 2114, 2183, 2621, 2842
Hpy99I	CGWCG	2 cut(s)	32, 317
HpyCH4IV	ACGT	5 cut(s)	693, 839, 1184, 1978, 2185
HpyF10VI	GCNNNNNNNGC	3 cut(s)	362, 887, 2558
HpyF3I	CTNAG	6 cut(s)	1167, 1476, 1590, 1728, 2027, 2049
HpySE526I	ACGT	5 cut(s)	693, 839, 1184, 1978, 2185
HspAI	GCGC	1 cut(s)	742
KflI	GGGWCCC	1 cut(s)	553
Kpn2I	TCCGGA	1 cut(s)	2887
KpnI	GGTACC	1 cut(s)	2975
LmnI	GCTCC	3 cut(s)	1921, 2659, 2900
Lsp1109I	GCAGC	4 cut(s)	1525, 2377, 2405, 2752
LweI	GCATC	6 cut(s)	390, 408, 1285, 2350, 2377, 2720
MaeI	CTAG	6 cut(s)	831, 2070, 2472, 2514, 2658, 3122
MaeII	ACGT	5 cut(s)	693, 839, 1184, 1978, 2185
MbiI	CCGCTC	2 cut(s)	472, 595
MflI	RGATCY	2 cut(s)	439, 1318
MhlI	GDGCHC	2 cut(s)	1589, 1963
MlsI	TGGCCA	2 cut(s)	756, 2688
MluI	ACGCGT	1 cut(s)	1678
MluNI	TGGCCA	2 cut(s)	756, 2688
MlyI	GAGTC	8 cut(s)	253, 1899, 2237, 2326, 2368, 2416, 2473, 2671
MmeI	TCCRAC	1 cut(s)	2205
Mox20I	TGGCCA	2 cut(s)	756, 2688
Mph1103I	ATGCAT	1 cut(s)	2343
MroI	TCCGGA	1 cut(s)	2887
MroXI	GAANNNNTTC	2 cut(s)	1203, 2883
MscI	TGGCCA	2 cut(s)	756, 2688
MslI	CAYNNNNRTG	2 cut(s)	1557, 1956
Msp20I	TGGCCA	2 cut(s)	756, 2688
MspCI	CTTAAG	1 cut(s)	1706
MspI	CCGG	3 cut(s)	59, 2888, 3027
Mva1269I	GAATGC	1 cut(s)	1694
MvnI	CGCG	2 cut(s)	1680, 2548
MwoI	GCNNNNNNNGC	3 cut(s)	362, 887, 2558
NciI	CCSGG	1 cut(s)	59
NdeI	CATATG	2 cut(s)	1624, 2554
NheI	GCTAGC	1 cut(s)	2513
NmeAIII	GCCGAG	1 cut(s)	325
NmuCI	GTSAC	5 cut(s)	8, 1594, 2278, 2432, 3009
NsbI	TGCGCA	1 cut(s)	743
NsiI	ATGCAT	1 cut(s)	2343
NspI	RCATGY	2 cut(s)	1520, 1928
PaeI	GCATGC	1 cut(s)	1928
PaeR7I	CTCGAG	1 cut(s)	2040
PagI	TCATGA	1 cut(s)	1633
PcsI	WCGNNNNNNNCGW	3 cut(s)	24, 81, 312
PctI	GAATGC	1 cut(s)	1694
PdmI	GAANNNNTTC	2 cut(s)	1203, 2883
PfeI	GAWTC	3 cut(s)	1450, 2152, 2446
PflMI	CCANNNNNTGG	3 cut(s)	1918, 2570, 2759
PfoI	TCCNGGA	1 cut(s)	2722
PinAI	ACCGGT	1 cut(s)	3026
PkrI	GCNGC	6 cut(s)	126, 909, 1515, 2392, 2395, 2742
PleI	GAGTC	8 cut(s)	252, 1898, 2236, 2326, 2368, 2416, 2472, 2671
PmaCI	CACGTG	1 cut(s)	1979
PmlI	CACGTG	1 cut(s)	1979
PpsI	GAGTC	8 cut(s)	252, 1898, 2236, 2326, 2368, 2416, 2472, 2671
Ppu21I	YACGTR	2 cut(s)	840, 1979
PpuMI	RGGWCCY	3 cut(s)	553, 1865, 2807
PshBI	ATTAAT	2 cut(s)	929, 1104
Psp124BI	GAGCTC	1 cut(s)	1589
Psp5II	RGGWCCY	3 cut(s)	553, 1865, 2807
PspCI	CACGTG	1 cut(s)	1979
PspFI	CCCAGC	1 cut(s)	1214
PspPI	GGNCC	9 cut(s)	61, 126, 267, 553, 1844, 1865, 2078, 2204, 2807
PspPPI	RGGWCCY	3 cut(s)	553, 1865, 2807
PspXI	VCTCGAGB	1 cut(s)	2040
PstI	CTGCAG	2 cut(s)	648, 2395
PstNI	CAGNNNCTG	1 cut(s)	2759
PsuI	RGATCY	2 cut(s)	439, 1318
RsaI	GTAC	5 cut(s)	203, 1165, 2214, 2620, 2973
RsaNI	GTAC	5 cut(s)	202, 1164, 2213, 2619, 2972
RseI	CAYNNNNRTG	2 cut(s)	1557, 1956
SacI	GAGCTC	1 cut(s)	1589
SatI	GCNGC	6 cut(s)	125, 908, 1514, 2391, 2394, 2741
Sau96I	GGNCC	9 cut(s)	61, 126, 267, 553, 1844, 1865, 2078, 2204, 2807
ScaI	AGTACT	1 cut(s)	1165
SchI	GAGTC	8 cut(s)	253, 1899, 2237, 2326, 2368, 2416, 2473, 2671
SduI	GDGCHC	2 cut(s)	1589, 1963
SfaNI	GCATC	6 cut(s)	390, 408, 1285, 2350, 2377, 2720
SfcI	CTRYAG	4 cut(s)	69, 644, 2391, 2899
Sfr274I	CTCGAG	1 cut(s)	2040
SinI	GGWCC	5 cut(s)	61, 553, 1865, 2204, 2807
SlaI	CTCGAG	1 cut(s)	2040
SmiMI	CAYNNNNRTG	2 cut(s)	1557, 1956
SmlI	CTYRAG	2 cut(s)	1706, 2040
SmoI	CTYRAG	2 cut(s)	1706, 2040
SphI	GCATGC	1 cut(s)	1928
SsiI	CCGC	9 cut(s)	111, 124, 139, 472, 595, 701, 907, 2548, 2640
SspI	AATATT	1 cut(s)	1027
SspMI	CTAG	6 cut(s)	831, 2070, 2472, 2514, 2658, 3122
SstI	GAGCTC	1 cut(s)	1589
StyI	CCWWGG	1 cut(s)	935
TaiI	ACGT	5 cut(s)	696, 842, 1187, 1981, 2188
TaqI	TCGA	8 cut(s)	288, 306, 409, 493, 630, 2041, 2350, 2675
TaqII	GACCGA	1 cut(s)	2451
TatI	WGTACW	1 cut(s)	1163
TauI	GCSGC	2 cut(s)	127, 910
TfiI	GAWTC	3 cut(s)	1450, 2152, 2446
TscAI	CASTG	3 cut(s)	2764, 2806, 2973
TseFI	GTSAC	5 cut(s)	8, 1594, 2278, 2432, 3009
TseI	GCWGC	4 cut(s)	1513, 2390, 2393, 2740
Tsp45I	GTSAC	5 cut(s)	8, 1594, 2278, 2432, 3009
TspGWI	ACGGA	3 cut(s)	1547, 1804, 3072
TspRI	CASTG	3 cut(s)	2764, 2806, 2973
Van91I	CCANNNNNTGG	3 cut(s)	1918, 2570, 2759
Vha464I	CTTAAG	1 cut(s)	1706
VpaK11BI	GGWCC	5 cut(s)	61, 553, 1865, 2204, 2807
VspI	ATTAAT	2 cut(s)	929, 1104
XagI	CCTNNNNNAGG	3 cut(s)	881, 2574, 2787
XapI	RAATTY	3 cut(s)	1202, 1327, 2704
XbaI	TCTAGA	1 cut(s)	2069
XceI	RCATGY	2 cut(s)	1520, 1928
XhoI	CTCGAG	1 cut(s)	2040
XmiI	GTMKAC	1 cut(s)	690
XmnI	GAANNNNTTC	2 cut(s)	1203, 2883
XspI	CTAG	6 cut(s)	831, 2070, 2472, 2514, 2658, 3122
ZrmI	AGTACT	1 cut(s)	1165
Zsp2I	ATGCAT	1 cut(s)	2343

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.