Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Forward (+)

2559307 .. 2569053

Gene Structure 9747 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1013100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3147 bp

ATGGCTCGTCACAGAGACGAATATGACGACGAGATGGGCCCCGATGAAGAGGAAGAATACGAGCCCGACCAAGCTATAGACGAGGATGACGAGGAGGAAGAGGAGGAGGAACGAGGAGGGGGACGACAGCGGTCCGGTCAGAAGCGGAGGAGATCGGATTTCATAGACGATGTAGCAGAGGAGGACGATGACGAGGAGGAAGAGGAGGACTATGAGGATGACGAGCCTTATGGTGGTGGTGGTGGTGGTGCGAGTCGTAAGCGGAAGAATAAGAGGCCCTCTGGCTCGCAGTTTCTCGACATCGAGGCCGAGGTCGATACCGATGAAGAAGAAGAAGACGAGGAGGGCGAGGACGACTTTATTGCTGATAATGCACCTGATGTACCTGAAGATGAGGATGGTAGAAGGATGCGTCATCGTCCGTTGATGCCACAAGAAGATGAGCAAGAAGATTTTGAAGAGATTGCGAAAAGTATTCAAGCGCGGTATGCGAAGTCAAGTCATACAGAGTATGATGAAGACACCACTGATGTGGATCAGCAAGCACTTTTACCTTCTGTTAGGGACCCAAAGTTGTGGATGGTGAAATGTGCGATTGGCCGTGAGCGGGAGGTAGCTGTTTGCTTAATGCAAAAGTATATTGATAAACCTGAACTGCAGATCAGGTCTGTTATTGCTCTTGACCACCTTAAAAACTATGTATACGTTGAAGCGGATAAAGAAGCTCATGTGAGGGAGGCTTGCAAAGGTCTGCGCAATATATTTGGCCAGAAAATAATGCTTGTTCCAATTAGAGAAATGACTGACGTTCTTTCTGTTGAAAGCAAGGCTATTGATCTCTCTAGGGATACATGGGTCAGAATGAAGACTGGTACATATAAAGGAGACCTTGCTCAGGTTGCGGATGTGGACAATGTGCGGCAGAGAGTTACAGTTAAACTAATTCCAAGGATTGATTTACAAGCTCTTGCAAATAAAATGGATGGCAGAGAAGTTGTGAGAACAAAAGCATTTGTTCCTCCTCCACGTTTACTAAATATTGAGGAAGCCAGGGAACTGCATATTCTTGGAGAGCGTAAACAAGATCGGATGACTGGTGATTATTATCAGACAATTAATGGCATGATGTTTAAAGATGGTTTCCTGTATAAAGCAGTATCAATCAAATCAATTAGTACTCAGAACATACATCCAACTTTTGACGAACTTGAAAAATTTCGGAAGCCCAGCGAGAATGGTGATGGAGATATTGCCAGTCTGTCAACTTTATTTTCAAACAGAAAGAAGGGACACTTTCTGAAGGGTGATGCAGTTATTGTTGTCAAGGGAGATCTAAAAAATTTGAAAGGATGGGTTGAGAAAGTTGAGGAGGAGACTGTTCATATTAGACCCGAAATGAAGGAACTCCCAAGAACACTTGCCATAAATGGAAAAGAGCTTTGCAAATACTTTGAGCCTGGGAATCATGTGAAAGTTGTATCTGGCACTCAGGAAGGTGCAACTGGTATGGTTGTTAAGGTTGAGCAGCATGTTCTCATTATCTTATCTGATGTAACGAAAGAACATATCCGTGTGTTTGCTGATGATGTTGTGGAGAGCTCTGAGGTGACATCTGGTATTACCAGAATTGGGGCGTATGAGCTTCATGATCTCGTGCTACTAGATAACAATAGCTTTGGTGTAATCATACGTGTAGAAAGTGAAGCATTTCAGGTTCTTAAGGGAGTTCCTGATAGACCCGAGGTTGCCCTTGTCAAGTTAAGAGAGGTCAAATGCAAGATTGAGAAAAGTTTTCCTGTGGAAGTTAAGTATAAACACAAAGTATCTGTGAAGGATGTTGTGAGGGTCATTGATGGCCCTTGCGAGGGGAAACAGGGTCCTGTGCAACACATATACAGGGGAGTCTTGTTCATTTTTGATCGCCATCACCTGGAGCATGCAGGCTTTATCTGTGTTAAGTCTCATAATTGTGCTCTTGTGGGAGGTTCACGTGCTAGTGGTGATAGAAATGGTAACTCAAATTCAAGATATGATCACCTCAGAACTCCTCCTCGTGTTCCTCAGTCCCCGAATAGGTTTTCTAGAGGAGGCCCTCCATCTAACTATGGAGGAAGGAATAGAGGTGGACGAGGGCATGATGGCTTGGTTGGGACTACAGTAAAGATTCGCCAGGGTGCCTATAAGGGCTATCGTGGACGTGTTGTTGAGGTTAAGGGACCAAATGTACGGGTTGAGTTGGAGTCTCAAATGAAGGTTGTTACAGTTGATCGAAATTGTATTTCTGATAATGTGACTGTTACCACACCATATCGTGATACATCTCGCTATGGTATGGGAAGTGAGACTCCTATGCATCCTTCTCGAACTCCTCTCCACCCATATATGACTCCTATGAGGGATGCTGGAGCAACACCTATACATGATGGCATGAGGACTCCTATGCGTGACCGAGCATGGAATCCGTATGCACCTATGAGTCCAGCTAGGGATAACTGGGAGGAAGGAAACCCTGCTTCTTGGGGTGCTAGCCCACAGTATCAGCCAGGAAGTCCTCCTTCGCGAGCGTATGAAGCACCAACTCCCGGTTCTGGTTGGGCTAACACTCCTGGTGGAAATTACAGTGAAGCTGGCACACCGAGGGACAGTAGTTCTGGTTATGCAAATGCTCCTAGCCCTTACTTGCCATCGACTCCAGGTGGCCAGCCTATGACACCAAATTCGGCATCCTATCTTCCTGGAACTCCTGGAGGGCAGCCAATGACACCAGGCACTGGTGGTCTGGATATGTTGTCTCCTGTTATAGGTGGGGACAGTGAAGGACCGTGGTTCCTCCCAGACATATTGGTCAATGTCCGCAATTCTGGAGAGGAAACTACTGGAGTTGTGAGAGAAGTGCTTCCGGATGGCTCGTGTAGGGTAGCTATTGGGTCGGGTGGCAATGGGGAAACTATAGTGGCACTTCCTAATGAAATGGAGGCAGTGGTACCGAAGAAAAGTGATAAGATAAAGATCATGGGCGGGTCACTACGAGGAGCGACCGGTAAGCTGATTGGTGTTGATGGCACAGACGGAATTGTGAAGGTAGATGACACTCTTGATGTTAAGATATTAGACTTGGCTATTTTGTCGAAACTTGCTCAATCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

1049

Amino Acids

116.21

Weight (kDa)

5.17

Isoelectric Point (pI)

44.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5_N	PF11942	98 - 185	8.6e-17	Spt5 transcription elongation factor, acidic N-terminal
Spt5-NGN	PF03439	191 - 271	1.4e-26	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	277 - 406	9.9e-69	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	434 - 483	9.7e-22	Transcription elongation factor SPT5, second KOW domain
KOWx_SPT5	PF23037	542 - 593	5.4e-17	Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5	PF23291	604 - 654	5.2e-21	Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5	PF23290	715 - 758	1.7e-17	Transcription elongation factor SPT5, fifth KOW domain
KOW6_SPT51-2	PF23038	943 - 992	1.9e-26	Transcription elongation factor SPT5 homolog 1/2, KOW6 domain
KOW7_SPT5	PF23287	994 - 1045	8.8e-21	Transcription elongation factor SPT5, seventh KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2843
Acc16I	TGCGCA	1 cut(s)	755
Acc65I	GGTACC	1 cut(s)	2983
AccB1I	GGYRCC	2 cut(s)	2174, 2983
AccB7I	CCANNNNNTGG	2 cut(s)	1930, 2771
AccBSI	CCGCTC	1 cut(s)	607
AccI	GTMKAC	1 cut(s)	702
AccII	CGCG	2 cut(s)	484, 2560
AccIII	TCCGGA	1 cut(s)	2899
AclWI	GGATC	1 cut(s)	543
AcoI	YGGCCR	3 cut(s)	598, 766, 2698
AcsI	RAATTY	4 cut(s)	1214, 1339, 2020, 2716
AcuI	CTGAAG	2 cut(s)	408, 1319
AcvI	CACGTG	1 cut(s)	1991
AfaI	GTAC	5 cut(s)	384, 874, 1177, 2226, 2985
AflII	CTTAAG	1 cut(s)	1718
AflIII	ACRYGT	2 cut(s)	1690, 2197
AgeI	ACCGGT	1 cut(s)	3038
AgsI	TTSAA	8 cut(s)	458, 479, 710, 821, 1211, 1275, 1345, 2025
AjiI	CACGTC	1 cut(s)	2198
AjuI	GAANNNNNNNTTGG	2 cut(s)	2569, 2601
AleI	CACNNNNGTG	1 cut(s)	530
Alw21I	GWGCWC	2 cut(s)	1601, 1975
Alw26I	GTCTC	7 cut(s)	9, 879, 1367, 1965, 2247, 2336, 2796
AlwI	GGATC	1 cut(s)	543
AlwNI	CAGNNNCTG	1 cut(s)	2771
Ama87I	CYCGRG	1 cut(s)	1739
Aor13HI	TCCGGA	1 cut(s)	2899
AoxI	GGCC	8 cut(s)	37, 275, 306, 598, 766, 1855, 2089, 2698
ApaI	GGGCCC	1 cut(s)	41
ApeKI	GCWGC	2 cut(s)	1525, 2752
ApoI	RAATTY	4 cut(s)	1214, 1339, 2020, 2716
ArsI	GACNNNNNNTTYG	2 cut(s)	2701, 2733
AseI	ATTAAT	1 cut(s)	1116
AsiGI	ACCGGT	1 cut(s)	3038
Asp700I	GAANNNNTTC	3 cut(s)	1215, 1707, 2895
Asp718I	GGTACC	1 cut(s)	2983
AspLEI	GCGC	2 cut(s)	484, 756
AsuC2I	CCSGG	1 cut(s)	2583
AsuHPI	GGTGA	8 cut(s)	595, 1109, 1250, 1316, 1618, 1919, 2012, 2027
AsuNHI	GCTAGC	1 cut(s)	2525
AvaI	CYCGRG	1 cut(s)	1739
AvaII	GGWCC	5 cut(s)	132, 565, 1877, 2216, 2819
BaeGI	GKGCMC	1 cut(s)	41
BaeI	ACNNNNGTAYC	2 cut(s)	840, 873
BalI	TGGCCA	2 cut(s)	768, 2700
BanI	GGYRCC	2 cut(s)	2174, 2983
BanII	GRGCYC	3 cut(s)	41, 66, 1601
BauI	CACGAG	3 cut(s)	1652, 2052, 2908
BbrPI	CACGTG	1 cut(s)	1991
BbsI	GAAGAC	3 cut(s)	342, 525, 872
Bbv12I	GWGCWC	2 cut(s)	1601, 1975
BbvI	GCAGC	2 cut(s)	1537, 2764
BceAI	ACGGC	1 cut(s)	585
BcgI	CGANNNNNNTGC	4 cut(s)	344, 378, 2342, 2376
BciVI	GTATCC	1 cut(s)	841
BclI	TGATCA	1 cut(s)	2032
BcnI	CCSGG	1 cut(s)	2583
BcoDI	GTCTC	7 cut(s)	9, 879, 1367, 1965, 2247, 2336, 2796
BfaI	CTAG	7 cut(s)	843, 1661, 1995, 2082, 2484, 2526, 2670
BfmI	CTRYAG	4 cut(s)	75, 656, 2154, 2949
BfrI	CTTAAG	1 cut(s)	1718
BfuI	GTATCC	1 cut(s)	841
BglII	AGATCT	1 cut(s)	1330
BisI	GCNGC	3 cut(s)	920, 1526, 2753
BlsI	GCNGC	3 cut(s)	921, 1527, 2754
BmcAI	AGTACT	1 cut(s)	1177
Bme18I	GGWCC	5 cut(s)	132, 565, 1877, 2216, 2819
BmeT110I	CYCGRG	1 cut(s)	1739
BmgBI	CACGTC	1 cut(s)	2198
BmiI	GGNNCC	9 cut(s)	39, 40, 566, 567, 1878, 2176, 2217, 2828, 2985
BmrI	ACTGGG	1 cut(s)	2503
BmsI	GCATC	6 cut(s)	399, 417, 1297, 2362, 2389, 2732
BmtI	GCTAGC	1 cut(s)	2529
BmuI	ACTGGG	1 cut(s)	2503
BoxI	GACNNNNGTC	1 cut(s)	130
BpiI	GAAGAC	3 cut(s)	342, 525, 872
BpmI	CTGGAG	6 cut(s)	1952, 2424, 2676, 2766, 2883, 2898
Bpu10I	CCTNAGC	1 cut(s)	894
BpuMI	CCSGG	1 cut(s)	2583
BsaAI	YACGTR	2 cut(s)	1691, 1991
BsaBI	GATNNNNATC	4 cut(s)	534, 1104, 1923, 3008
BsaI	GGTCTC	1 cut(s)	879
BsaJI	CCNNGG	8 cut(s)	309, 947, 1050, 1457, 1740, 2170, 2636, 2822
BsaWI	WCCGGW	3 cut(s)	134, 2899, 3038
Bse118I	RCCGGY	1 cut(s)	3038
Bse1I	ACTGG	7 cut(s)	874, 1099, 1254, 1507, 2498, 2776, 2881
Bse3DI	GCAATG	1 cut(s)	2944
Bse8I	GATNNNNATC	4 cut(s)	534, 1104, 1923, 3008
BseAI	TCCGGA	1 cut(s)	2899
BseDI	CCNNGG	8 cut(s)	309, 947, 1050, 1457, 1740, 2170, 2636, 2822
BseJI	GATNNNNATC	4 cut(s)	534, 1104, 1923, 3008
BseMI	GCAATG	1 cut(s)	2944
BseMII	CTCAG	6 cut(s)	908, 1193, 1502, 1593, 2053, 2075
BseNI	ACTGG	7 cut(s)	874, 1099, 1254, 1507, 2498, 2776, 2881
BseSI	GKGCMC	1 cut(s)	41
BseXI	GCAGC	2 cut(s)	1537, 2764
BseYI	CCCAGC	1 cut(s)	1226
Bsh1236I	CGCG	2 cut(s)	484, 2560
Bsh1285I	CGRYCG	1 cut(s)	3039
BshFI	GGCC	8 cut(s)	39, 277, 308, 600, 768, 1857, 2091, 2700
BshNI	GGYRCC	2 cut(s)	2174, 2983
BshTI	ACCGGT	1 cut(s)	3038
BsiEI	CGRYCG	1 cut(s)	3039
BsiHKAI	GWGCWC	2 cut(s)	1601, 1975
BsiHKCI	CYCGRG	1 cut(s)	1739
BsiSI	CCGG	4 cut(s)	135, 2583, 2900, 3039
BslFI	GGGAC	8 cut(s)	135, 578, 1302, 2050, 2164, 2229, 2654, 2822
BsmAI	GTCTC	7 cut(s)	9, 879, 1367, 1965, 2247, 2336, 2796
BsmBI	CGTCTC	1 cut(s)	9
BsmFI	GGGAC	8 cut(s)	135, 578, 1302, 2050, 2164, 2229, 2654, 2822
BsnI	GGCC	8 cut(s)	39, 277, 308, 600, 768, 1857, 2091, 2700
Bso31I	GGTCTC	1 cut(s)	879
BsoBI	CYCGRG	1 cut(s)	1739
Bsp120I	GGGCCC	1 cut(s)	37
Bsp1286I	GDGCHC	4 cut(s)	41, 66, 1601, 1975
Bsp13I	TCCGGA	1 cut(s)	2899
Bsp68I	TCGCGA	1 cut(s)	2560
BspANI	GGCC	8 cut(s)	39, 277, 308, 600, 768, 1857, 2091, 2700
BspCNI	CTCAG	6 cut(s)	907, 1192, 1501, 1594, 2052, 2074
BspEI	TCCGGA	1 cut(s)	2899
BspFNI	CGCG	2 cut(s)	484, 2560
BspHI	TCATGA	1 cut(s)	1645
BspLI	GGNNCC	9 cut(s)	39, 40, 566, 567, 1878, 2176, 2217, 2828, 2985
BspMAI	CTGCAG	1 cut(s)	660
BspOI	GCTAGC	1 cut(s)	2529
BspPI	GGATC	1 cut(s)	543
BspT107I	GGYRCC	2 cut(s)	2174, 2983
BspTI	CTTAAG	1 cut(s)	1718
BspTNI	GGTCTC	1 cut(s)	879
BsrBI	CCGCTC	1 cut(s)	607
BsrDI	GCAATG	1 cut(s)	2944
BsrFI	RCCGGY	1 cut(s)	3038
BsrI	ACTGG	7 cut(s)	874, 1099, 1254, 1507, 2498, 2776, 2881
BssAI	RCCGGY	1 cut(s)	3038
BssECI	CCNNGG	8 cut(s)	309, 947, 1050, 1457, 1740, 2170, 2636, 2822
BssNAI	GTATAC	1 cut(s)	703
BssSI	CACGAG	3 cut(s)	1652, 2052, 2908
BssT1I	CCWWGG	1 cut(s)	947
Bst1107I	GTATAC	1 cut(s)	703
Bst2BI	CACGAG	3 cut(s)	1652, 2052, 2908
Bst6I	CTCTTC	4 cut(s)	42, 93, 195, 453
BstAFI	CTTAAG	1 cut(s)	1718
BstBAI	YACGTR	2 cut(s)	1691, 1991
BstC8I	GCNNGC	9 cut(s)	287, 543, 742, 1938, 1942, 2527, 2562, 2629, 2702
BstDEI	CTNAG	6 cut(s)	894, 1179, 1488, 1602, 2039, 2061
BstDSI	CCRYGG	1 cut(s)	2822
BstENI	CCTNNNNNAGG	2 cut(s)	893, 2799
BstFNI	CGCG	2 cut(s)	484, 2560
BstHHI	GCGC	2 cut(s)	484, 756
BstMAI	GTCTC	7 cut(s)	9, 879, 1367, 1965, 2247, 2336, 2796
BstMCI	CGRYCG	1 cut(s)	3039
BstMWI	GCNNNNNNNGC	4 cut(s)	371, 488, 899, 2570
BstNSI	RCATGY	2 cut(s)	1532, 1940
BstPAI	GACNNNNGTC	1 cut(s)	130
BstSFI	CTRYAG	4 cut(s)	75, 656, 2154, 2949
BstSLI	GKGCMC	1 cut(s)	41
BstUI	CGCG	2 cut(s)	484, 2560
BstV1I	GCAGC	2 cut(s)	1537, 2764
BstV2I	GAAGAC	3 cut(s)	342, 525, 872
BstX2I	RGATCY	1 cut(s)	1330
BstXI	CCANNNNNNTGG	2 cut(s)	532, 576
BstYI	RGATCY	1 cut(s)	1330
BstZ17I	GTATAC	1 cut(s)	703
BsuI	GTATCC	1 cut(s)	841
BsuRI	GGCC	8 cut(s)	39, 277, 308, 600, 768, 1857, 2091, 2700
BtgI	CCRYGG	1 cut(s)	2822
BtrI	CACGTC	1 cut(s)	2198
BtsI	GCAGTG	1 cut(s)	2985
BtsIMutI	CAGTG	5 cut(s)	525, 2626, 2769, 2818, 2985
BtuMI	TCGCGA	1 cut(s)	2560
Cac8I	GCNNGC	9 cut(s)	287, 543, 742, 1938, 1942, 2527, 2562, 2629, 2702
CaiI	CAGNNNCTG	1 cut(s)	2771
CciI	TCATGA	1 cut(s)	1645
CfoI	GCGC	2 cut(s)	484, 756
Cfr10I	RCCGGY	1 cut(s)	3038
CpoI	CGGWCCG	1 cut(s)	132
CseI	GACGC	1 cut(s)	401
Csp6I	GTAC	5 cut(s)	383, 873, 1176, 2225, 2984
CspAI	ACCGGT	1 cut(s)	3038
CspI	CGGWCCG	1 cut(s)	132
CviQI	GTAC	5 cut(s)	383, 873, 1176, 2225, 2984
DdeI	CTNAG	6 cut(s)	894, 1179, 1488, 1602, 2039, 2061
DraI	TTTAAA	1 cut(s)	1132
DrdI	GACNNNNNNGTC	1 cut(s)	2843
DseDI	GACNNNNNNGTC	1 cut(s)	2843
EaeI	YGGCCR	3 cut(s)	598, 766, 2698
Eam1104I	CTCTTC	4 cut(s)	42, 93, 195, 453
EarI	CTCTTC	4 cut(s)	42, 93, 195, 453
Ecl136II	GAGCTC	1 cut(s)	1599
Eco130I	CCWWGG	1 cut(s)	947
Eco24I	GRGCYC	3 cut(s)	41, 66, 1601
Eco31I	GGTCTC	1 cut(s)	879
Eco47I	GGWCC	5 cut(s)	132, 565, 1877, 2216, 2819
Eco53kI	GAGCTC	1 cut(s)	1599
Eco57I	CTGAAG	2 cut(s)	408, 1319
Eco72I	CACGTG	1 cut(s)	1991
Eco88I	CYCGRG	1 cut(s)	1739
EcoICRI	GAGCTC	1 cut(s)	1599
EcoNI	CCTNNNNNAGG	2 cut(s)	893, 2799
EcoO109I	RGGNCCY	5 cut(s)	38, 276, 565, 1877, 2090
EcoT14I	CCWWGG	1 cut(s)	947
EcoT22I	ATGCAT	1 cut(s)	2355
EcoT38I	GRGCYC	3 cut(s)	41, 66, 1601
ErhI	CCWWGG	1 cut(s)	947
Esp3I	CGTCTC	1 cut(s)	9
FalI	AAGNNNNNCTT	6 cut(s)	873, 905, 1277, 1309, 2539, 2571
FaqI	GGGAC	8 cut(s)	135, 578, 1302, 2050, 2164, 2229, 2654, 2822
FauI	CCCGC	2 cut(s)	600, 3011
FbaI	TGATCA	1 cut(s)	2032
FblI	GTMKAC	1 cut(s)	702
Fnu4HI	GCNGC	3 cut(s)	920, 1526, 2753
FriOI	GRGCYC	3 cut(s)	41, 66, 1601
Fsp4HI	GCNGC	3 cut(s)	920, 1526, 2753
FspBI	CTAG	7 cut(s)	843, 1661, 1995, 2082, 2484, 2526, 2670
FspI	TGCGCA	1 cut(s)	755
GlaI	GCGC	2 cut(s)	483, 755
GluI	GCNGC	3 cut(s)	920, 1526, 2753
GsaI	CCCAGC	1 cut(s)	1230
GsuI	CTGGAG	6 cut(s)	1952, 2424, 2676, 2766, 2883, 2898
HaeIII	GGCC	8 cut(s)	39, 277, 308, 600, 768, 1857, 2091, 2700
HapII	CCGG	4 cut(s)	135, 2583, 2900, 3039
HgaI	GACGC	1 cut(s)	401
HhaI	GCGC	2 cut(s)	484, 756
Hin6I	GCGC	2 cut(s)	482, 754
HinP1I	GCGC	2 cut(s)	482, 754
HincII	GTYRAC	1 cut(s)	1263
HindII	GTYRAC	1 cut(s)	1263
HpaII	CCGG	4 cut(s)	135, 2583, 2900, 3039
HphI	GGTGA	8 cut(s)	595, 1109, 1250, 1316, 1618, 1919, 2012, 2027
Hpy166II	GTNNAC	8 cut(s)	703, 910, 1031, 1079, 1263, 1988, 2126, 2195
Hpy8I	GTNNAC	8 cut(s)	703, 910, 1031, 1079, 1263, 1988, 2126, 2195
Hpy99I	CGWCG	1 cut(s)	32
HpyCH4IV	ACGT	6 cut(s)	705, 807, 1027, 1690, 1990, 2197
HpyF10VI	GCNNNNNNNGC	4 cut(s)	371, 488, 899, 2570
HpyF3I	CTNAG	6 cut(s)	894, 1179, 1488, 1602, 2039, 2061
HpySE526I	ACGT	6 cut(s)	705, 807, 1027, 1690, 1990, 2197
HspAI	GCGC	2 cut(s)	482, 754
KflI	GGGWCCC	1 cut(s)	565
Kpn2I	TCCGGA	1 cut(s)	2899
KpnI	GGTACC	1 cut(s)	2987
Ksp22I	TGATCA	1 cut(s)	2032
LmnI	GCTCC	4 cut(s)	1933, 2405, 2671, 3032
Lsp1109I	GCAGC	2 cut(s)	1537, 2764
LweI	GCATC	6 cut(s)	399, 417, 1297, 2362, 2389, 2732
MaeI	CTAG	7 cut(s)	843, 1661, 1995, 2082, 2484, 2526, 2670
MaeII	ACGT	6 cut(s)	705, 807, 1027, 1690, 1990, 2197
MbiI	CCGCTC	1 cut(s)	607
MflI	RGATCY	1 cut(s)	1330
MhlI	GDGCHC	4 cut(s)	41, 66, 1601, 1975
MlsI	TGGCCA	2 cut(s)	768, 2700
MluNI	TGGCCA	2 cut(s)	768, 2700
MlyI	GAGTC	8 cut(s)	262, 1911, 2249, 2338, 2380, 2428, 2485, 2683
MmeI	TCCRAC	2 cut(s)	1217, 2217
Mox20I	TGGCCA	2 cut(s)	768, 2700
Mph1103I	ATGCAT	1 cut(s)	2355
MroI	TCCGGA	1 cut(s)	2899
MroXI	GAANNNNTTC	3 cut(s)	1215, 1707, 2895
MscI	TGGCCA	2 cut(s)	768, 2700
MslI	CAYNNNNRTG	3 cut(s)	530, 1569, 1968
Msp20I	TGGCCA	2 cut(s)	768, 2700
MspA1I	CMGCKG	1 cut(s)	130
MspCI	CTTAAG	1 cut(s)	1718
MspI	CCGG	4 cut(s)	135, 2583, 2900, 3039
MvnI	CGCG	2 cut(s)	484, 2560
MwoI	GCNNNNNNNGC	4 cut(s)	371, 488, 899, 2570
NciI	CCSGG	1 cut(s)	2583
NheI	GCTAGC	1 cut(s)	2525
NlaIV	GGNNCC	9 cut(s)	39, 40, 566, 567, 1878, 2176, 2217, 2828, 2985
NmeAIII	GCCGAG	1 cut(s)	334
NmuCI	GTSAC	5 cut(s)	8, 1606, 2290, 2444, 3021
NruI	TCGCGA	1 cut(s)	2560
NsbI	TGCGCA	1 cut(s)	755
NsiI	ATGCAT	1 cut(s)	2355
NspI	RCATGY	2 cut(s)	1532, 1940
OliI	CACNNNNGTG	1 cut(s)	530
PaeI	GCATGC	1 cut(s)	1940
PagI	TCATGA	1 cut(s)	1645
PcsI	WCGNNNNNNNCGW	3 cut(s)	24, 87, 345
PdmI	GAANNNNTTC	3 cut(s)	1215, 1707, 2895
PfeI	GAWTC	3 cut(s)	1462, 2164, 2458
PflMI	CCANNNNNTGG	2 cut(s)	1930, 2771
PfoI	TCCNGGA	2 cut(s)	2734, 2743
PinAI	ACCGGT	1 cut(s)	3038
PkrI	GCNGC	3 cut(s)	921, 1527, 2754
PleI	GAGTC	8 cut(s)	261, 1910, 2248, 2338, 2380, 2428, 2484, 2683
PmaCI	CACGTG	1 cut(s)	1991
PmlI	CACGTG	1 cut(s)	1991
PpsI	GAGTC	8 cut(s)	261, 1910, 2248, 2338, 2380, 2428, 2484, 2683
Ppu21I	YACGTR	2 cut(s)	1691, 1991
PpuMI	RGGWCCY	2 cut(s)	565, 1877
PshAI	GACNNNNGTC	1 cut(s)	130
PshBI	ATTAAT	1 cut(s)	1116
Psp124BI	GAGCTC	1 cut(s)	1601
Psp5II	RGGWCCY	2 cut(s)	565, 1877
PspCI	CACGTG	1 cut(s)	1991
PspFI	CCCAGC	1 cut(s)	1226
PspN4I	GGNNCC	9 cut(s)	39, 40, 566, 567, 1878, 2176, 2217, 2828, 2985
PspOMI	GGGCCC	1 cut(s)	37
PspPPI	RGGWCCY	2 cut(s)	565, 1877
PstI	CTGCAG	1 cut(s)	660
PstNI	CAGNNNCTG	1 cut(s)	2771
PsuI	RGATCY	1 cut(s)	1330
RruI	TCGCGA	1 cut(s)	2560
RsaI	GTAC	5 cut(s)	384, 874, 1177, 2226, 2985
RsaNI	GTAC	5 cut(s)	383, 873, 1176, 2225, 2984
RseI	CAYNNNNRTG	3 cut(s)	530, 1569, 1968
Rsr2I	CGGWCCG	1 cut(s)	132
RsrII	CGGWCCG	1 cut(s)	132
SacI	GAGCTC	1 cut(s)	1601
SatI	GCNGC	3 cut(s)	920, 1526, 2753
ScaI	AGTACT	1 cut(s)	1177
SchI	GAGTC	8 cut(s)	262, 1911, 2249, 2338, 2380, 2428, 2485, 2683
SduI	GDGCHC	4 cut(s)	41, 66, 1601, 1975
SfaNI	GCATC	6 cut(s)	399, 417, 1297, 2362, 2389, 2732
SfcI	CTRYAG	4 cut(s)	75, 656, 2154, 2949
SinI	GGWCC	5 cut(s)	132, 565, 1877, 2216, 2819
SmiMI	CAYNNNNRTG	3 cut(s)	530, 1569, 1968
SmlI	CTYRAG	1 cut(s)	1718
SmoI	CTYRAG	1 cut(s)	1718
SphI	GCATGC	1 cut(s)	1940
SspI	AATATT	1 cut(s)	1039
SspMI	CTAG	7 cut(s)	843, 1661, 1995, 2082, 2484, 2526, 2670
SstI	GAGCTC	1 cut(s)	1601
StyI	CCWWGG	1 cut(s)	947
TaiI	ACGT	6 cut(s)	708, 810, 1030, 1693, 1993, 2200
TaqI	TCGA	7 cut(s)	297, 303, 315, 2269, 2362, 2687, 3128
TaqII	GACCGA	1 cut(s)	2463
TatI	WGTACW	1 cut(s)	1175
TauI	GCSGC	1 cut(s)	922
TfiI	GAWTC	3 cut(s)	1462, 2164, 2458
TscAI	CASTG	5 cut(s)	532, 2626, 2776, 2818, 2985
TseFI	GTSAC	5 cut(s)	8, 1606, 2290, 2444, 3021
TseI	GCWGC	2 cut(s)	1525, 2752
Tsp45I	GTSAC	5 cut(s)	8, 1606, 2290, 2444, 3021
TspGWI	ACGGA	4 cut(s)	411, 1559, 2451, 3084
TspRI	CASTG	5 cut(s)	532, 2626, 2776, 2818, 2985
Van91I	CCANNNNNTGG	2 cut(s)	1930, 2771
Vha464I	CTTAAG	1 cut(s)	1718
VpaK11BI	GGWCC	5 cut(s)	132, 565, 1877, 2216, 2819
VspI	ATTAAT	1 cut(s)	1116
XagI	CCTNNNNNAGG	2 cut(s)	893, 2799
XapI	RAATTY	4 cut(s)	1214, 1339, 2020, 2716
XbaI	TCTAGA	1 cut(s)	2081
XceI	RCATGY	2 cut(s)	1532, 1940
XmiI	GTMKAC	1 cut(s)	702
XmnI	GAANNNNTTC	3 cut(s)	1215, 1707, 2895
XspI	CTAG	7 cut(s)	843, 1661, 1995, 2082, 2484, 2526, 2670
ZrmI	AGTACT	1 cut(s)	1177
Zsp2I	ATGCAT	1 cut(s)	2355

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.