Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Forward (+)

16947788 .. 16952495

Gene Structure 4708 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027930

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2370 bp

ATGTCGAATTGGAGAGAGGATTATCGAGAAGACGAGAGCGAGATGCGGGAGGAGAGGAGAGGAGGACGAGGGGGGAATTCGAAGCGAGGGAGATTGAATTCTGACGAGATTGAGATCCAGAAGCGGAGAGGGCGAGGGTCGAATTCGAAAAAGAGGAAATTGAAATCGGTTGCGGAGGAGGATGAGGGGGAGGAGGAAGAAGAAGAAGAAGGCGGTGGTGGAAGGCCTTTTAAGTCGGTGTTTGTGGATGATGAGGCTATAGAGGATGAAGATGAGGATGAGGAGGAAGACGATGAGGGTGAATTGGATGGGTTTATTGTTAATGAACCGGAGTTTGATAACGAACCTGATGAAGTTGAACATGATGGTGGTGGTGGGAGAAGGATGGGGTTTGTGTTGCCGGAGGAAGAAGAGGAGGAAGATATTGATGAGTTTGAAAGAAGGGTTCAAGACAGATTTAGGGATGTTCATGTGCAAGAATATGATGATGAAACAGAGGTTGGACAGCAAGCTCTTATACCCAGTGTTAAGGACCCGAAATTGTGGTTGGTGAAGTGTGCCATTGGTAAAGAGAGAGAGGTAGCCGCTTGTCTAATGCAGAAGTGTATTGATAACCCTGGAGAATTGCAGATCATGTCTGCCATTGCTTTGGATCACTTGAAAAACTTCATATATGTTGAAGCTTACAAAGAAGCCCATGTGAGGGAAGCTTGCAAAGGTCTGAGGTATGTAATTCTGCAGAAAATAATGCTTGTTCCTATTAAAGAGATGATTGATGTGCTATCATGTGAAAGCAAGAGCGTTGTAAATGATAAATGGGTTAGGATTAAGACGGGAGTATACAAAGGGGACCTTGCCAAGATTGTGGGCCATGTCCCGGGGAGACAGCAAGTTAGAGTAAAGTTAATTCCAAGGGTTGATTTACAAGCTCTTGCGAAGGGTGTAGAATCATTGAAGAAGAAGGGATCTGGTGCACGTCCTGATCGTGTTCCTGCACTTCTTAGGAACATGAACCTAGTTAGACAGCTGGGTGTACGAGTACAGTCCATGCGAGATCCATTGGCACGTGGTTATATTGAGAAGATTGATGGCCTATCTGGGATGATCTTTAAAGATGGTTTCCATCTTAAAGTTGTTTCTTATAAAAGTATAAAAGAGGCAAAACCCTCCTTGCAAGAACTTGAAGAATTCCAGGAACTTGATGGTGATGGAGATCATACAATTCCAAATAAAAAGAAAGGCGAAAGTTTTATGAAGGGTGATGTGGTTGTTGTTATGGAGGGAGATCTGAGAAACTTGGTGGGACGGGTTGACAAAGTTGAGTTAGGGACTGTTCATATCAAACCGGAAATGGATGGCCTCCCAGAAACTGTTGCCATCAATCAAACAGCACTTCAGAAATGCTTTAAAGAAGGACAGCATGTGAAGGTTGTAAGTGGTAGTCAACAAGGAGCAACTGGCATGGTTGTTAAGGTTGATCAGCAGAAGCAGTTGATCACCATTTTATCTGATGCAGCCAATGAAGCTATCTGCATGTTCTCTGATCATGTTGTGGAGAGTCCTGAAGTGAATAATGGTGATACAATGGGTGACTCCCACAGCTTAAGGTATCACAAAACCCCTGGTCGAAGTCCGAGGTTTTCTGGAGGTGCAAGAAGGCATGATGGGCAGGGTGGTTTTGGTAGAGGGAGCCGGGTAAAATTCCGGCTAGGTCCCTATAAGGGCTGCAGGGGGCGTGTTGTGGAGGTTAAAGGATCAAATGTAAGGGTTGAATTAGAGTCCCAGATGAAAACTGTTGTTGTTGACCGTATGCATTGTATTTCAGACTCTAGTAACGTGGCTGCTGCAGTAACAACACCATATCGCTCGGGTTGTGAGACCCCAATGCCTTACATGAGGACTCCTATGAGAGATCCTGGAGCAATGTCAATGACCTACATGACGACTCCCATGAGAGATCCCGGAGCATCCCCAATGCGTTATATGATGACTCCCAGAAGAGATCCCCCCGAGCAACACCAATACATGATTCATGAAAGTTTGCAGAGGATACGTGAAAGAAAGCTTCTTAACTTTATTGAGTGGGCCCCTGCAAGCATTCAGGTCAGTGGTCTTATGCTAGCCAGCCATACTAGTATTCGCCACCTTTTCAGCAAGTGTTTGAGCCAGTATTCGTTGGTAAGAAGGAAGCAAGCTTTTCTTGACAACTACAGGAAGTTCCCAATGTTTGCTTGTTTGGTTGATGAATATAAGGCCCGCCAGTCCCCAGATTTCATCAAATGGGGAATGGAGACCACGTTCTTACAGGAGAAGGCAGTGCAGCAGGAACAGTGGACCCAAAAGTGGCAGTATGAACAATTTAGAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

790

Amino Acids

89.77

Weight (kDa)

5.86

Isoelectric Point (pI)

49.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5-NGN	PF03439	180 - 261	5.7e-24	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	269 - 397	7e-27	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	419 - 468	1e-15	Transcription elongation factor SPT5, second KOW domain
KOW5_SPT5	PF23290	563 - 605	8.8e-13	Transcription elongation factor SPT5, fifth KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1143
AccI	GTMKAC	1 cut(s)	840
AciI	CCGC	6 cut(s)	46, 124, 173, 213, 585, 2257
AclWI	GGATC	8 cut(s)	109, 660, 973, 1049, 1762, 1907, 1952, 1997
AcsI	RAATTY	5 cut(s)	76, 97, 142, 1187, 1700
AcuI	CTGAAG	2 cut(s)	1379, 1584
AcvI	CACGTG	1 cut(s)	1067
AfaI	GTAC	2 cut(s)	1035, 1041
AfiI	CCNNNNNNNGG	5 cut(s)	702, 703, 877, 1721, 2343
AflII	CTTAAG	1 cut(s)	1603
AhlI	ACTAGT	1 cut(s)	2132
AjiI	CACGTC	1 cut(s)	977
AjnI	CCWGG	4 cut(s)	616, 1191, 1621, 1915
AjuI	GAANNNNNNNTTGG	6 cut(s)	483, 515, 530, 562, 2013, 2045
AloI	GAACNNNNNNTCC	2 cut(s)	2282, 2314
AluBI	AGCT	9 cut(s)	512, 683, 710, 929, 1027, 1526, 1602, 2065, 2195
AluI	AGCT	9 cut(s)	512, 683, 710, 929, 1027, 1526, 1602, 2065, 2195
Alw21I	GWGCWC	1 cut(s)	976
Alw26I	GTCTC	3 cut(s)	877, 1871, 2285
Alw44I	GTGCAC	1 cut(s)	972
AlwI	GGATC	8 cut(s)	109, 660, 973, 1049, 1762, 1907, 1952, 1997
AlwNI	CAGNNNCTG	1 cut(s)	1370
Ama87I	CYCGRG	3 cut(s)	877, 1867, 2009
AoxI	GGCC	6 cut(s)	224, 868, 1090, 1357, 2085, 2253
ApaI	GGGCCC	1 cut(s)	2089
ApaLI	GTGCAC	1 cut(s)	972
ApeKI	GCWGC	5 cut(s)	1514, 1725, 1841, 1844, 2320
ApoI	RAATTY	5 cut(s)	76, 97, 142, 1187, 1700
Asp700I	GAANNNNTTC	1 cut(s)	665
AspS9I	GGNCC	8 cut(s)	532, 850, 868, 1712, 2085, 2086, 2254, 2334
AsuC2I	CCSGG	4 cut(s)	878, 879, 1694, 1962
AsuHPI	GGTGA	7 cut(s)	311, 562, 1217, 1271, 1489, 1589, 1601
AsuII	TTCGAA	2 cut(s)	80, 146
AsuNHI	GCTAGC	1 cut(s)	2119
AvaI	CYCGRG	3 cut(s)	877, 1867, 2009
AvaII	GGWCC	4 cut(s)	532, 850, 1712, 2334
BaeGI	GKGCMC	2 cut(s)	976, 2089
BanII	GRGCYC	1 cut(s)	2089
BbrPI	CACGTG	1 cut(s)	1067
BbsI	GAAGAC	2 cut(s)	36, 294
Bbv12I	GWGCWC	1 cut(s)	976
BbvI	GCAGC	5 cut(s)	1526, 1712, 1828, 1831, 2332
BciT130I	CCWGG	4 cut(s)	618, 1193, 1623, 1917
BciVI	GTATCC	1 cut(s)	2043
BclI	TGATCA	3 cut(s)	1477, 1494, 1543
BcnI	CCSGG	4 cut(s)	878, 879, 1694, 1962
BcoDI	GTCTC	3 cut(s)	877, 1871, 2285
BcuI	ACTAGT	1 cut(s)	2132
BfaI	CTAG	5 cut(s)	1016, 1709, 1830, 2120, 2133
BfmI	CTRYAG	5 cut(s)	258, 737, 1726, 1845, 2209
BfrI	CTTAAG	1 cut(s)	1603
BfuI	GTATCC	1 cut(s)	2043
BglII	AGATCT	1 cut(s)	1285
BisI	GCNGC	6 cut(s)	585, 1515, 1726, 1842, 1845, 2321
BlsI	GCNGC	6 cut(s)	586, 1516, 1727, 1843, 1846, 2322
Bme1390I	CCNGG	8 cut(s)	618, 878, 879, 1193, 1623, 1694, 1917, 1962
Bme18I	GGWCC	4 cut(s)	532, 850, 1712, 2334
BmeT110I	CYCGRG	3 cut(s)	877, 1867, 2009
BmgBI	CACGTC	1 cut(s)	977
BmgT120I	GGNCC	8 cut(s)	532, 850, 868, 1712, 2085, 2086, 2254, 2334
BmiI	GGNNCC	7 cut(s)	534, 851, 1691, 1714, 2087, 2088, 2336
BmrFI	CCNGG	8 cut(s)	618, 878, 879, 1193, 1623, 1694, 1917, 1962
BmrI	ACTGGG	1 cut(s)	516
BmsI	GCATC	3 cut(s)	33, 1501, 1976
BmtI	GCTAGC	1 cut(s)	2123
BmuI	ACTGGG	1 cut(s)	516
BpiI	GAAGAC	2 cut(s)	36, 294
BpmI	CTGGAG	3 cut(s)	639, 1665, 1938
Bpu14I	TTCGAA	2 cut(s)	80, 146
BpuMI	CCSGG	4 cut(s)	878, 879, 1694, 1962
BsaAI	YACGTR	2 cut(s)	1067, 2054
BsaBI	GATNNNNATC	2 cut(s)	113, 1212
BsaI	GGTCTC	2 cut(s)	1871, 2285
BsaJI	CCNNGG	6 cut(s)	616, 877, 878, 911, 1621, 1634
BsaWI	WCCGGW	2 cut(s)	328, 1345
BsaXI	ACNNNNNCTCC	2 cut(s)	2282, 2312
Bsc4I	CCNNNNNNNGG	5 cut(s)	702, 703, 877, 1721, 2343
Bse1I	ACTGG	4 cut(s)	522, 1462, 2167, 2260
Bse3DI	GCAATG	2 cut(s)	642, 1929
Bse8I	GATNNNNATC	2 cut(s)	113, 1212
BseBI	CCWGG	4 cut(s)	618, 1193, 1623, 1917
BseDI	CCNNGG	6 cut(s)	616, 877, 878, 911, 1621, 1634
BseJI	GATNNNNATC	2 cut(s)	113, 1212
BseLI	CCNNNNNNNGG	5 cut(s)	702, 703, 877, 1721, 2343
BseMI	GCAATG	2 cut(s)	642, 1929
BseMII	CTCAG	2 cut(s)	713, 1280
BseNI	ACTGG	4 cut(s)	522, 1462, 2167, 2260
BseRI	GAGGAG	7 cut(s)	65, 70, 75, 191, 206, 296, 428
BseSI	GKGCMC	2 cut(s)	976, 2089
BseXI	GCAGC	5 cut(s)	1526, 1712, 1828, 1831, 2332
BseYI	CCCAGC	1 cut(s)	1027
BsgI	GTGCAG	2 cut(s)	978, 2339
BshFI	GGCC	6 cut(s)	226, 870, 1092, 1359, 2087, 2255
BsiHKAI	GWGCWC	1 cut(s)	976
BsiHKCI	CYCGRG	3 cut(s)	877, 1867, 2009
BsiSI	CCGG	7 cut(s)	329, 401, 878, 1346, 1693, 1705, 1962
BslFI	GGGAC	7 cut(s)	860, 863, 1317, 1342, 1698, 1765, 2248
BslI	CCNNNNNNNGG	5 cut(s)	702, 703, 877, 1721, 2343
BsmAI	GTCTC	3 cut(s)	877, 1871, 2285
BsmFI	GGGAC	7 cut(s)	860, 863, 1317, 1342, 1698, 1765, 2248
BsmI	GAATGC	1 cut(s)	2097
BsnI	GGCC	6 cut(s)	226, 870, 1092, 1359, 2087, 2255
Bso31I	GGTCTC	2 cut(s)	1871, 2285
BsoBI	CYCGRG	3 cut(s)	877, 1867, 2009
Bsp119I	TTCGAA	2 cut(s)	80, 146
Bsp120I	GGGCCC	1 cut(s)	2085
Bsp1286I	GDGCHC	2 cut(s)	976, 2089
BspACI	CCGC	6 cut(s)	46, 124, 173, 213, 585, 2257
BspANI	GGCC	6 cut(s)	226, 870, 1092, 1359, 2087, 2255
BspCNI	CTCAG	2 cut(s)	714, 1281
BspHI	TCATGA	1 cut(s)	2032
BspLI	GGNNCC	7 cut(s)	534, 851, 1691, 1714, 2087, 2088, 2336
BspMAI	CTGCAG	3 cut(s)	741, 1730, 1849
BspOI	GCTAGC	1 cut(s)	2123
BspPI	GGATC	8 cut(s)	109, 660, 973, 1049, 1762, 1907, 1952, 1997
BspT104I	TTCGAA	2 cut(s)	80, 146
BspTI	CTTAAG	1 cut(s)	1603
BspTNI	GGTCTC	2 cut(s)	1871, 2285
BsrDI	GCAATG	2 cut(s)	642, 1929
BsrI	ACTGG	4 cut(s)	522, 1462, 2167, 2260
BssECI	CCNNGG	6 cut(s)	616, 877, 878, 911, 1621, 1634
BssNAI	GTATAC	1 cut(s)	841
BssT1I	CCWWGG	1 cut(s)	911
Bst1107I	GTATAC	1 cut(s)	841
Bst2UI	CCWGG	4 cut(s)	618, 1193, 1623, 1917
Bst4CI	ACNGT	6 cut(s)	1044, 1333, 1372, 1795, 1808, 2331
Bst6I	CTCTTC	2 cut(s)	405, 1993
BstAFI	CTTAAG	1 cut(s)	1603
BstBAI	YACGTR	2 cut(s)	1067, 2054
BstBI	TTCGAA	2 cut(s)	80, 146
BstC8I	GCNNGC	7 cut(s)	510, 712, 2095, 2121, 2125, 2193, 2257
BstDEI	CTNAG	3 cut(s)	722, 1001, 1289
BstMAI	GTCTC	3 cut(s)	877, 1871, 2285
BstMWI	GCNNNNNNNGC	3 cut(s)	130, 1523, 1666
BstNI	CCWGG	4 cut(s)	618, 1193, 1623, 1917
BstNSI	RCATGY	2 cut(s)	1424, 1537
BstSCI	CCNGG	8 cut(s)	616, 876, 877, 1191, 1621, 1692, 1915, 1960
BstSFI	CTRYAG	5 cut(s)	258, 737, 1726, 1845, 2209
BstSLI	GKGCMC	2 cut(s)	976, 2089
BstV1I	GCAGC	5 cut(s)	1526, 1712, 1828, 1831, 2332
BstV2I	GAAGAC	2 cut(s)	36, 294
BstX2I	RGATCY	7 cut(s)	114, 965, 1054, 1285, 1912, 1957, 2002
BstXI	CCANNNNNNTGG	2 cut(s)	649, 865
BstYI	RGATCY	7 cut(s)	114, 965, 1054, 1285, 1912, 1957, 2002
BstZ17I	GTATAC	1 cut(s)	841
BsuI	GTATCC	1 cut(s)	2043
BsuRI	GGCC	6 cut(s)	226, 870, 1092, 1359, 2087, 2255
BtrI	CACGTC	1 cut(s)	977
BtsI	GCAGTG	1 cut(s)	2322
BtsIMutI	CAGTG	4 cut(s)	529, 2113, 2322, 2336
Cac8I	GCNNGC	7 cut(s)	510, 712, 2095, 2121, 2125, 2193, 2257
CaiI	CAGNNNCTG	1 cut(s)	1370
CciI	TCATGA	1 cut(s)	2032
Cfr13I	GGNCC	8 cut(s)	532, 850, 868, 1712, 2085, 2086, 2254, 2334
Cfr9I	CCCGGG	1 cut(s)	877
Csp6I	GTAC	2 cut(s)	1034, 1040
CviQI	GTAC	2 cut(s)	1034, 1040
DdeI	CTNAG	3 cut(s)	722, 1001, 1289
DraI	TTTAAA	2 cut(s)	1111, 1408
Eam1104I	CTCTTC	2 cut(s)	405, 1993
EarI	CTCTTC	2 cut(s)	405, 1993
Eco130I	CCWWGG	1 cut(s)	911
Eco147I	AGGCCT	1 cut(s)	226
Eco24I	GRGCYC	1 cut(s)	2089
Eco31I	GGTCTC	2 cut(s)	1871, 2285
Eco47I	GGWCC	4 cut(s)	532, 850, 1712, 2334
Eco57I	CTGAAG	2 cut(s)	1379, 1584
Eco72I	CACGTG	1 cut(s)	1067
Eco88I	CYCGRG	3 cut(s)	877, 1867, 2009
EcoO109I	RGGNCCY	4 cut(s)	532, 850, 1712, 2086
EcoRI	GAATTC	4 cut(s)	76, 97, 142, 1187
EcoRII	CCWGG	4 cut(s)	616, 1191, 1621, 1915
EcoT14I	CCWWGG	1 cut(s)	911
EcoT22I	ATGCAT	1 cut(s)	1815
EcoT38I	GRGCYC	1 cut(s)	2089
ErhI	CCWWGG	1 cut(s)	911
FalI	AAGNNNNNCTT	4 cut(s)	837, 869, 2184, 2216
FaqI	GGGAC	7 cut(s)	860, 863, 1317, 1342, 1698, 1765, 2248
FauI	CCCGC	2 cut(s)	39, 2264
FbaI	TGATCA	3 cut(s)	1477, 1494, 1543
FblI	GTMKAC	1 cut(s)	840
Fnu4HI	GCNGC	6 cut(s)	585, 1515, 1726, 1842, 1845, 2321
FriOI	GRGCYC	1 cut(s)	2089
Fsp4HI	GCNGC	6 cut(s)	585, 1515, 1726, 1842, 1845, 2321
FspBI	CTAG	5 cut(s)	1016, 1709, 1830, 2120, 2133
GluI	GCNGC	6 cut(s)	585, 1515, 1726, 1842, 1845, 2321
GsaI	CCCAGC	1 cut(s)	1031
GsuI	CTGGAG	3 cut(s)	639, 1665, 1938
HaeIII	GGCC	6 cut(s)	226, 870, 1092, 1359, 2087, 2255
HapII	CCGG	7 cut(s)	329, 401, 878, 1346, 1693, 1705, 1962
HincII	GTYRAC	3 cut(s)	1312, 1445, 1804
HindII	GTYRAC	3 cut(s)	1312, 1445, 1804
HindIII	AAGCTT	4 cut(s)	681, 708, 2063, 2193
HinfI	GANTC	9 cut(s)	947, 1558, 1592, 1778, 1826, 1900, 1945, 1990, 2029
HpaII	CCGG	7 cut(s)	329, 401, 878, 1346, 1693, 1705, 1962
HphI	GGTGA	7 cut(s)	311, 562, 1217, 1271, 1489, 1589, 1601
Hpy166II	GTNNAC	7 cut(s)	841, 974, 1034, 1312, 1445, 1804, 2334
Hpy188I	TCNGA	8 cut(s)	103, 723, 1290, 1398, 1510, 1543, 1635, 1825
Hpy188III	TCNNGA	8 cut(s)	26, 118, 449, 980, 1562, 1644, 2033, 2201
Hpy8I	GTNNAC	7 cut(s)	841, 974, 1034, 1312, 1445, 1804, 2334
HpyCH4III	ACNGT	6 cut(s)	1044, 1333, 1372, 1795, 1808, 2331
HpyCH4IV	ACGT	5 cut(s)	976, 1066, 1836, 2053, 2297
HpyF10VI	GCNNNNNNNGC	3 cut(s)	130, 1523, 1666
HpyF3I	CTNAG	3 cut(s)	722, 1001, 1289
HpySE526I	ACGT	5 cut(s)	976, 1066, 1836, 2053, 2297
Ksp22I	TGATCA	3 cut(s)	1477, 1494, 1543
LmnI	GCTCC	4 cut(s)	1451, 1689, 1919, 1964
Lsp1109I	GCAGC	5 cut(s)	1526, 1712, 1828, 1831, 2332
LweI	GCATC	3 cut(s)	33, 1501, 1976
MaeI	CTAG	5 cut(s)	1016, 1709, 1830, 2120, 2133
MaeII	ACGT	5 cut(s)	976, 1066, 1836, 2053, 2297
MaeIII	GTNAC	3 cut(s)	1589, 1832, 1849
MflI	RGATCY	7 cut(s)	114, 965, 1054, 1285, 1912, 1957, 2002
MhlI	GDGCHC	2 cut(s)	976, 2089
MlyI	GAGTC	7 cut(s)	1567, 1586, 1787, 1820, 1894, 1939, 1984
MmeI	TCCRAC	1 cut(s)	481
Mph1103I	ATGCAT	1 cut(s)	1815
MroXI	GAANNNNTTC	1 cut(s)	665
MslI	CAYNNNNRTG	1 cut(s)	366
MspA1I	CMGCKG	1 cut(s)	1027
MspCI	CTTAAG	1 cut(s)	1603
MspI	CCGG	7 cut(s)	329, 401, 878, 1346, 1693, 1705, 1962
MspR9I	CCNGG	8 cut(s)	618, 878, 879, 1193, 1623, 1694, 1917, 1962
Mva1269I	GAATGC	1 cut(s)	2097
MvaI	CCWGG	4 cut(s)	618, 1193, 1623, 1917
MwoI	GCNNNNNNNGC	3 cut(s)	130, 1523, 1666
NciI	CCSGG	4 cut(s)	878, 879, 1694, 1962
NheI	GCTAGC	1 cut(s)	2119
NlaIV	GGNNCC	7 cut(s)	534, 851, 1691, 1714, 2087, 2088, 2336
NmuCI	GTSAC	1 cut(s)	1589
NsiI	ATGCAT	1 cut(s)	1815
NspI	RCATGY	2 cut(s)	1424, 1537
NspV	TTCGAA	2 cut(s)	80, 146
PagI	TCATGA	1 cut(s)	2032
PceI	AGGCCT	1 cut(s)	226
PcsI	WCGNNNNNNNCGW	1 cut(s)	982
PctI	GAATGC	1 cut(s)	2097
PdmI	GAANNNNTTC	1 cut(s)	665
PfeI	GAWTC	2 cut(s)	947, 2029
PfoI	TCCNGGA	3 cut(s)	1191, 1915, 1960
PkrI	GCNGC	6 cut(s)	586, 1516, 1727, 1843, 1846, 2322
PleI	GAGTC	7 cut(s)	1566, 1586, 1786, 1820, 1894, 1939, 1984
PmaCI	CACGTG	1 cut(s)	1067
PmlI	CACGTG	1 cut(s)	1067
PpsI	GAGTC	7 cut(s)	1566, 1586, 1786, 1820, 1894, 1939, 1984
Ppu21I	YACGTR	2 cut(s)	1067, 2054
PpuMI	RGGWCCY	3 cut(s)	532, 850, 1712
PsiI	TTATAA	1 cut(s)	1143
Psp5II	RGGWCCY	3 cut(s)	532, 850, 1712
Psp6I	CCWGG	4 cut(s)	616, 1191, 1621, 1915
PspCI	CACGTG	1 cut(s)	1067
PspFI	CCCAGC	1 cut(s)	1027
PspGI	CCWGG	4 cut(s)	616, 1191, 1621, 1915
PspN4I	GGNNCC	7 cut(s)	534, 851, 1691, 1714, 2087, 2088, 2336
PspOMI	GGGCCC	1 cut(s)	2085
PspPI	GGNCC	8 cut(s)	532, 850, 868, 1712, 2085, 2086, 2254, 2334
PspPPI	RGGWCCY	3 cut(s)	532, 850, 1712
PstI	CTGCAG	3 cut(s)	741, 1730, 1849
PstNI	CAGNNNCTG	1 cut(s)	1370
PsuI	RGATCY	7 cut(s)	114, 965, 1054, 1285, 1912, 1957, 2002
PvuII	CAGCTG	1 cut(s)	1027
RsaI	GTAC	2 cut(s)	1035, 1041
RsaNI	GTAC	2 cut(s)	1034, 1040
RseI	CAYNNNNRTG	1 cut(s)	366
SatI	GCNGC	6 cut(s)	585, 1515, 1726, 1842, 1845, 2321
Sau96I	GGNCC	8 cut(s)	532, 850, 868, 1712, 2085, 2086, 2254, 2334
SchI	GAGTC	7 cut(s)	1567, 1586, 1787, 1820, 1894, 1939, 1984
ScrFI	CCNGG	8 cut(s)	618, 878, 879, 1193, 1623, 1694, 1917, 1962
SduI	GDGCHC	2 cut(s)	976, 2089
SfaNI	GCATC	3 cut(s)	33, 1501, 1976
SfcI	CTRYAG	5 cut(s)	258, 737, 1726, 1845, 2209
SfuI	TTCGAA	2 cut(s)	80, 146
SinI	GGWCC	4 cut(s)	532, 850, 1712, 2334
SmaI	CCCGGG	1 cut(s)	879
SmiMI	CAYNNNNRTG	1 cut(s)	366
SmlI	CTYRAG	1 cut(s)	1603
SmoI	CTYRAG	1 cut(s)	1603
SpeI	ACTAGT	1 cut(s)	2132
SseBI	AGGCCT	1 cut(s)	226
SsiI	CCGC	6 cut(s)	46, 124, 173, 213, 585, 2257
SspMI	CTAG	5 cut(s)	1016, 1709, 1830, 2120, 2133
StuI	AGGCCT	1 cut(s)	226
StyD4I	CCNGG	8 cut(s)	616, 876, 877, 1191, 1621, 1692, 1915, 1960
StyI	CCWWGG	1 cut(s)	911
TaaI	ACNGT	6 cut(s)	1044, 1333, 1372, 1795, 1808, 2331
TaiI	ACGT	5 cut(s)	979, 1069, 1839, 2056, 2300
TaqI	TCGA	6 cut(s)	5, 25, 80, 140, 146, 1627
TatI	WGTACW	1 cut(s)	1039
TauI	GCSGC	1 cut(s)	587
TfiI	GAWTC	2 cut(s)	947, 2029
TscAI	CASTG	4 cut(s)	529, 2113, 2322, 2336
TseFI	GTSAC	1 cut(s)	1589
TseI	GCWGC	5 cut(s)	1514, 1725, 1841, 1844, 2320
Tsp45I	GTSAC	1 cut(s)	1589
TspMI	CCCGGG	1 cut(s)	877
TspRI	CASTG	4 cut(s)	529, 2113, 2322, 2336
Vha464I	CTTAAG	1 cut(s)	1603
VneI	GTGCAC	1 cut(s)	972
VpaK11BI	GGWCC	4 cut(s)	532, 850, 1712, 2334
XapI	RAATTY	5 cut(s)	76, 97, 142, 1187, 1700
XceI	RCATGY	2 cut(s)	1424, 1537
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1199
XmaI	CCCGGG	1 cut(s)	877
XmiI	GTMKAC	1 cut(s)	840
XmnI	GAANNNNTTC	1 cut(s)	665
XspI	CTAG	5 cut(s)	1016, 1709, 1830, 2120, 2133
Zsp2I	ATGCAT	1 cut(s)	1815

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.