Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Forward (+)

17510844 .. 17519372

Gene Structure 8529 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00297640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2406 bp

ATGGAGCCTCATCAACACTCGGACCCCTACGAGGATCCTCGACGCCACGCAGCCAGAAGAAGAGGACGAAGAGCTCTACGAATACGAACCGGTTCGGCTTGGCTCGAACCGATTCGTAATCGTGGTCCTCCTCAATCTCCTTCTTCTCGCGGTTTTTCTCGTTCTTATCCTTGGATTGGGCTTGGCGGCGTCGAGAGCCATGTCGAATTGGAGAGAGGATTATGGGGAAGACGAGGGCGAGATGCGGGAGGAGAGGAGAGGAGGACGAGGGGGGATTCGAAGCGGGGGAGATTGAATTCTGACGAGATTGAGATCCAGAGGCGGAGAGGGCGAGGGTCGAATTCGAAAAAGAGGAAATTGAAATCGGTTGCGGAGGAGGAAGAAGGCGGTGGTGGAAGGCCTTTTAAGTCGATGTTTGTGGATGATGAGGCTATAGAGGATGAAGATGAGGATGAGGAGGAAGACGATGATGGTGAATTGGATGGGTTTATTGTTAATGAACCGGAGTTTGATAACGAACCTGATGAAGTTGATCATGATGGTGGCGGTGGGAGAAGGATGGGGTTTGTGTTGCCGGAGGAAGAAGAGGAGGAAGATATTGAAGAGTTTGAAAGAAGGGTTCAGGACAGATTTAGGGATGTTCATGTGGAAGAATATGAGGATGATACGGAGGTTGGACAGCAAGCTCTTATACCCGGTGTTAAGGACCCGAAATTGTGGTTGGTGAAGTGTGCCATTGGTAAAGAGAGAGAGGTAGCAGCTTGTCTAATGCAGAAGTGTATTGATAACCCTGGAGAATTGCAGATCATGTCTGCAATTGCTTTGGATCACTTGAAAAGCTTCATATATGTTGAAGCTTACAAAGAAGCCCATGTGAGGGAAGCTTGCAAAGGTCTGAGGTATGTAATTCTGCAGAAAATAATGCTGGTTCCTATTAAAGAGATGATTGATGTGCTATCATGTGAAAGCAAGAGCATTGTAAATGATAAATGGGTTAGGATTAAGACTGGAATATATAAAGGGGACCTTGCCAAGATTGTGGGGCATGTCCCGGGGAGACAGCAAGTTAGAGTAAAGTTAATTCCAAGGGTTGATTTACAAGCTCTTGCAAAAGGTGTAGAATCATCGAAGAAGAAGGAATCTGGTGCATGTCCTGAACGTGTTCCTGCACTACTGAAGAACATGAACCTGGTTAGACAGCTGGGTATTCGAGTACAGTCTATGCGAGATCCATTGGCACGTGGGTATATTGAGAAGATTGATGGCTTATCTGGGATGATCTTTAAAGATGGTTTCCATCTTAAAGTTGTTTCTGATAAAAGTATAAAAGAGGCAAAACCCTCCTTGCAAGAACTTGAAGAATTCCAGGAACTCGATGGTGATGGAGATCATACAATTCCAAATAGAAAGAAAGGCGAAAGCTTTATGAAGGGCAATGCAGTTATTGTTACGGAGGGAGATCTGAGAAACTTGGTGGGACGGGTTGAGAAAGTTGAATTAGGGATTGTTCATATCAAAGCGGAAATGGATGGCCTCCCGGAAACTGTTGCCATCAATCAAACATCACTTCAGAAATGCTTTAAAGAAGGGCAGCATGTGAAGGTTGTTAGTGGTAGTCAACAAGGAGCAACTGGCATGGTTGTTAAGGTTGATCAGCAGAAGCAGTTAATCACCATTTTATCTGATGCAACCAAGGAAGCTATCTGCGTGTTCTCTGATCATGCTGTGGAGAGTCCTGAAATAAATAATGGTGATACAATGGGTGACTCCCACTGCTTAAGGTATCACAAAATCCCTAGTCAAAGTTCGAGGTTTTCTGGAGGTGCAAGAAGGCATGATGGGCAGGATGGTTTTGGTAAAGGGAGCCGAGTAAAGTTCCGTCTAGGTCCCTATAAAGGCTGTAGGGGGCGTGTTGTGGAGGTTAAAGGATCAAATGTAAGGGTTGAACTAGAGTCCCAGATGAAAACTGTTGTTGTTGATCGTCTGCATTGTATTTCAGAATCAGGTAATATGGCTGCTGCAGTTACAACACCATATCGCTCTGGTTGTGAGACCCCAATGCCTTACGTGAGGACTCCTATGAGAGATCCTGGAGCAACGTCAATGCCTTACATGACAACTCCCATGAGAGATCCTGGAGCATCCCCAATGCGTTATATGACGACTCCTTACATGATGACTCCCAGAAGAGATCCCCCCAAGCAACACCAATACATGGTTCATGAAAGTTTGCAGAGGATACTTGAAAGAAAGCTTCTTAACTTTATTGAGTGGGCCCCTGCAAGCATTCAGGTTGCGTTGTCTAGGAAGTCTCCATATGTGCAAACTGGCCATAGGGTCAGTGGTCTTATGCTAGCCAGCCATACTAGTATTCGCCACCTTTTCAGCAAGTGTTTGAGACTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

801

Amino Acids

90.16

Weight (kDa)

7.88

Isoelectric Point (pI)

52.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5-NGN	PF03439	238 - 319	3.4e-24	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	327 - 455	1.4e-27	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	477 - 526	4.2e-15	Transcription elongation factor SPT5, second KOW domain
KOW5_SPT5	PF23290	621 - 661	2.7e-12	Transcription elongation factor SPT5, fifth KOW domain
Tubulin_C	PF03953	738 - 798	1.6e-16	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	2215
AccII	CGCG	1 cut(s)	150
AciI	CCGC	9 cut(s)	150, 186, 245, 283, 322, 371, 387, 546, 1520
AclWI	GGATC	9 cut(s)	29, 42, 307, 834, 1223, 1936, 2081, 2126, 2186
AcoI	YGGCCR	1 cut(s)	2329
AcsI	RAATTY	3 cut(s)	295, 340, 1361
AcuI	CTGAAG	2 cut(s)	1196, 1553
AcvI	CACGTG	1 cut(s)	1241
AcyI	GRCGYC	2 cut(s)	43, 189
AfaI	GTAC	1 cut(s)	1215
AfiI	CCNNNNNNNGG	6 cut(s)	31, 176, 876, 877, 1895, 2215
AflII	CTTAAG	1 cut(s)	1777
AflIII	ACRYGT	1 cut(s)	1159
AgeI	ACCGGT	1 cut(s)	89
AhlI	ACTAGT	1 cut(s)	2366
AjnI	CCWGG	5 cut(s)	790, 1188, 1365, 2089, 2134
AjuI	GAANNNNNNNTTGG	4 cut(s)	704, 736, 2202, 2234
Alw21I	GWGCWC	1 cut(s)	76
Alw26I	GTCTC	4 cut(s)	1051, 2045, 2316, 2392
AlwI	GGATC	9 cut(s)	29, 42, 307, 834, 1223, 1936, 2081, 2126, 2186
Ama87I	CYCGRG	1 cut(s)	1051
AoxI	GGCC	4 cut(s)	398, 1531, 2274, 2329
ApaI	GGGCCC	1 cut(s)	2278
ApeKI	GCWGC	5 cut(s)	50, 758, 1591, 2015, 2018
ApoI	RAATTY	3 cut(s)	295, 340, 1361
AsiGI	ACCGGT	1 cut(s)	89
Asp700I	GAANNNNTTC	4 cut(s)	91, 111, 839, 1161
AspS9I	GGNCC	7 cut(s)	22, 125, 706, 1024, 1886, 2274, 2275
AsuC2I	CCSGG	4 cut(s)	696, 1052, 1053, 1538
AsuHPI	GGTGA	6 cut(s)	485, 736, 1391, 1663, 1763, 1775
AsuII	TTCGAA	2 cut(s)	278, 344
AsuNHI	GCTAGC	1 cut(s)	2353
AvaI	CYCGRG	1 cut(s)	1051
AvaII	GGWCC	5 cut(s)	22, 125, 706, 1024, 1886
BaeGI	GKGCMC	1 cut(s)	2278
BaeI	ACNNNNGTAYC	2 cut(s)	657, 690
BalI	TGGCCA	1 cut(s)	2331
BamHI	GGATCC	1 cut(s)	34
BanII	GRGCYC	2 cut(s)	76, 2278
BbrPI	CACGTG	1 cut(s)	1241
BbsI	GAAGAC	2 cut(s)	235, 468
Bbv12I	GWGCWC	1 cut(s)	76
BbvI	GCAGC	5 cut(s)	62, 770, 1603, 2002, 2005
BciT130I	CCWGG	5 cut(s)	792, 1190, 1367, 2091, 2136
BciVI	GTATCC	1 cut(s)	2232
BclI	TGATCA	3 cut(s)	532, 1651, 1717
BcnI	CCSGG	4 cut(s)	696, 1052, 1053, 1538
BcoDI	GTCTC	4 cut(s)	1051, 2045, 2316, 2392
BcuI	ACTAGT	1 cut(s)	2366
BfaI	CTAG	6 cut(s)	1797, 1883, 1949, 2304, 2354, 2367
BfmI	CTRYAG	4 cut(s)	432, 911, 1900, 2019
BfrI	CTTAAG	1 cut(s)	1777
BfuI	GTATCC	1 cut(s)	2232
BglII	AGATCT	1 cut(s)	1459
BisI	GCNGC	6 cut(s)	51, 187, 759, 1592, 2016, 2019
BlsI	GCNGC	6 cut(s)	52, 188, 760, 1593, 2017, 2020
Bme1390I	CCNGG	9 cut(s)	696, 792, 1052, 1053, 1190, 1367, 1538, 2091, 2136
Bme18I	GGWCC	5 cut(s)	22, 125, 706, 1024, 1886
BmeT110I	CYCGRG	1 cut(s)	1051
BmgT120I	GGNCC	7 cut(s)	22, 125, 706, 1024, 1886, 2274, 2275
BmrFI	CCNGG	9 cut(s)	696, 792, 1052, 1053, 1190, 1367, 1538, 2091, 2136
BmsI	GCATC	3 cut(s)	232, 1675, 2150
BmtI	GCTAGC	1 cut(s)	2357
BpiI	GAAGAC	2 cut(s)	235, 468
BpmI	CTGGAG	4 cut(s)	813, 1839, 2112, 2157
Bpu14I	TTCGAA	2 cut(s)	278, 344
BpuMI	CCSGG	4 cut(s)	696, 1052, 1053, 1538
BsaAI	YACGTR	2 cut(s)	1241, 2068
BsaBI	GATNNNNATC	2 cut(s)	311, 1386
BsaHI	GRCGYC	2 cut(s)	43, 189
BsaI	GGTCTC	1 cut(s)	2045
BsaJI	CCNNGG	6 cut(s)	170, 790, 1051, 1052, 1085, 1692
BsaWI	WCCGGW	2 cut(s)	89, 502
Bsc4I	CCNNNNNNNGG	6 cut(s)	31, 176, 876, 877, 1895, 2215
Bse118I	RCCGGY	1 cut(s)	89
Bse1I	ACTGG	3 cut(s)	1012, 1636, 2332
Bse3DI	GCAATG	1 cut(s)	1441
Bse8I	GATNNNNATC	2 cut(s)	311, 1386
BseBI	CCWGG	5 cut(s)	792, 1190, 1367, 2091, 2136
BseDI	CCNNGG	6 cut(s)	170, 790, 1051, 1052, 1085, 1692
BseJI	GATNNNNATC	2 cut(s)	311, 1386
BseLI	CCNNNNNNNGG	6 cut(s)	31, 176, 876, 877, 1895, 2215
BseMI	GCAATG	1 cut(s)	1441
BseMII	CTCAG	2 cut(s)	887, 1454
BseNI	ACTGG	3 cut(s)	1012, 1636, 2332
BseRI	GAGGAG	7 cut(s)	120, 264, 269, 274, 389, 470, 602
BseSI	GKGCMC	1 cut(s)	2278
BseXI	GCAGC	5 cut(s)	62, 770, 1603, 2002, 2005
BseYI	CCCAGC	1 cut(s)	1201
BsgI	GTGCAG	1 cut(s)	1152
Bsh1236I	CGCG	1 cut(s)	150
BshFI	GGCC	4 cut(s)	400, 1533, 2276, 2331
BshTI	ACCGGT	1 cut(s)	89
BsiHKAI	GWGCWC	1 cut(s)	76
BsiHKCI	CYCGRG	1 cut(s)	1051
BsiSI	CCGG	6 cut(s)	90, 503, 575, 696, 1052, 1538
BslFI	GGGAC	5 cut(s)	1034, 1037, 1491, 1872, 1939
BslI	CCNNNNNNNGG	6 cut(s)	31, 176, 876, 877, 1895, 2215
BsmAI	GTCTC	4 cut(s)	1051, 2045, 2316, 2392
BsmFI	GGGAC	5 cut(s)	1034, 1037, 1491, 1872, 1939
BsmI	GAATGC	1 cut(s)	2286
BsnI	GGCC	4 cut(s)	400, 1533, 2276, 2331
Bso31I	GGTCTC	1 cut(s)	2045
BsoBI	CYCGRG	1 cut(s)	1051
Bsp119I	TTCGAA	2 cut(s)	278, 344
Bsp120I	GGGCCC	1 cut(s)	2274
Bsp1286I	GDGCHC	2 cut(s)	76, 2278
BspACI	CCGC	9 cut(s)	150, 186, 245, 283, 322, 371, 387, 546, 1520
BspANI	GGCC	4 cut(s)	400, 1533, 2276, 2331
BspCNI	CTCAG	2 cut(s)	888, 1455
BspFNI	CGCG	1 cut(s)	150
BspHI	TCATGA	2 cut(s)	535, 2221
BspMAI	CTGCAG	2 cut(s)	915, 2023
BspOI	GCTAGC	1 cut(s)	2357
BspPI	GGATC	9 cut(s)	29, 42, 307, 834, 1223, 1936, 2081, 2126, 2186
BspQI	GCTCTTC	1 cut(s)	64
BspT104I	TTCGAA	2 cut(s)	278, 344
BspTI	CTTAAG	1 cut(s)	1777
BspTNI	GGTCTC	1 cut(s)	2045
BsrDI	GCAATG	1 cut(s)	1441
BsrFI	RCCGGY	1 cut(s)	89
BsrI	ACTGG	3 cut(s)	1012, 1636, 2332
BssAI	RCCGGY	1 cut(s)	89
BssECI	CCNNGG	6 cut(s)	170, 790, 1051, 1052, 1085, 1692
BssNI	GRCGYC	2 cut(s)	43, 189
BssT1I	CCWWGG	3 cut(s)	170, 1085, 1692
Bst2UI	CCWGG	5 cut(s)	792, 1190, 1367, 2091, 2136
Bst4CI	ACNGT	3 cut(s)	1218, 1546, 1969
Bst6I	CTCTTC	5 cut(s)	55, 64, 579, 597, 2182
BstACI	GRCGYC	2 cut(s)	43, 189
BstAFI	CTTAAG	1 cut(s)	1777
BstBAI	YACGTR	2 cut(s)	1241, 2068
BstBI	TTCGAA	2 cut(s)	278, 344
BstC8I	GCNNGC	5 cut(s)	684, 886, 2284, 2355, 2359
BstDEI	CTNAG	2 cut(s)	896, 1463
BstFNI	CGCG	1 cut(s)	150
BstMAI	GTCTC	4 cut(s)	1051, 2045, 2316, 2392
BstMWI	GCNNNNNNNGC	3 cut(s)	195, 328, 1840
BstNI	CCWGG	5 cut(s)	792, 1190, 1367, 2091, 2136
BstNSI	RCATGY	3 cut(s)	1049, 1152, 1598
BstSCI	CCNGG	9 cut(s)	694, 790, 1050, 1051, 1188, 1365, 1536, 2089, 2134
BstSFI	CTRYAG	4 cut(s)	432, 911, 1900, 2019
BstSLI	GKGCMC	1 cut(s)	2278
BstUI	CGCG	1 cut(s)	150
BstV1I	GCAGC	5 cut(s)	62, 770, 1603, 2002, 2005
BstV2I	GAAGAC	2 cut(s)	235, 468
BstX2I	RGATCY	7 cut(s)	34, 312, 1228, 1459, 2086, 2131, 2191
BstXI	CCANNNNNNTGG	1 cut(s)	1039
BstYI	RGATCY	7 cut(s)	34, 312, 1228, 1459, 2086, 2131, 2191
BsuI	GTATCC	1 cut(s)	2232
BsuRI	GGCC	4 cut(s)	400, 1533, 2276, 2331
BtsI	GCAGTG	1 cut(s)	1771
BtsIMutI	CAGTG	2 cut(s)	1771, 2347
Cac8I	GCNNGC	5 cut(s)	684, 886, 2284, 2355, 2359
CciI	TCATGA	2 cut(s)	535, 2221
Cfr10I	RCCGGY	1 cut(s)	89
Cfr13I	GGNCC	7 cut(s)	22, 125, 706, 1024, 1886, 2274, 2275
Cfr9I	CCCGGG	1 cut(s)	1051
CseI	GACGC	2 cut(s)	51, 178
CsiI	ACCWGGT	1 cut(s)	1188
Csp6I	GTAC	1 cut(s)	1214
CspAI	ACCGGT	1 cut(s)	89
CviQI	GTAC	1 cut(s)	1214
DdeI	CTNAG	2 cut(s)	896, 1463
DraI	TTTAAA	2 cut(s)	1285, 1582
EaeI	YGGCCR	1 cut(s)	2329
Eam1104I	CTCTTC	5 cut(s)	55, 64, 579, 597, 2182
EarI	CTCTTC	5 cut(s)	55, 64, 579, 597, 2182
EciI	GGCGGA	1 cut(s)	337
Ecl136II	GAGCTC	1 cut(s)	74
Eco130I	CCWWGG	3 cut(s)	170, 1085, 1692
Eco147I	AGGCCT	1 cut(s)	400
Eco24I	GRGCYC	2 cut(s)	76, 2278
Eco31I	GGTCTC	1 cut(s)	2045
Eco47I	GGWCC	5 cut(s)	22, 125, 706, 1024, 1886
Eco53kI	GAGCTC	1 cut(s)	74
Eco57I	CTGAAG	2 cut(s)	1196, 1553
Eco72I	CACGTG	1 cut(s)	1241
Eco88I	CYCGRG	1 cut(s)	1051
EcoICRI	GAGCTC	1 cut(s)	74
EcoO109I	RGGNCCY	4 cut(s)	706, 1024, 1886, 2275
EcoRI	GAATTC	3 cut(s)	295, 340, 1361
EcoRII	CCWGG	5 cut(s)	790, 1188, 1365, 2089, 2134
EcoT14I	CCWWGG	3 cut(s)	170, 1085, 1692
EcoT38I	GRGCYC	2 cut(s)	76, 2278
ErhI	CCWWGG	3 cut(s)	170, 1085, 1692
FalI	AAGNNNNNCTT	2 cut(s)	1011, 1043
FaqI	GGGAC	5 cut(s)	1034, 1037, 1491, 1872, 1939
FauI	CCCGC	2 cut(s)	238, 276
FauNDI	CATATG	1 cut(s)	2317
FbaI	TGATCA	3 cut(s)	532, 1651, 1717
Fnu4HI	GCNGC	6 cut(s)	51, 187, 759, 1592, 2016, 2019
FriOI	GRGCYC	2 cut(s)	76, 2278
Fsp4HI	GCNGC	6 cut(s)	51, 187, 759, 1592, 2016, 2019
FspBI	CTAG	6 cut(s)	1797, 1883, 1949, 2304, 2354, 2367
GluI	GCNGC	6 cut(s)	51, 187, 759, 1592, 2016, 2019
GsaI	CCCAGC	1 cut(s)	1205
GsuI	CTGGAG	4 cut(s)	813, 1839, 2112, 2157
HaeIII	GGCC	4 cut(s)	400, 1533, 2276, 2331
HapII	CCGG	6 cut(s)	90, 503, 575, 696, 1052, 1538
HgaI	GACGC	2 cut(s)	51, 178
Hin1I	GRCGYC	2 cut(s)	43, 189
HincII	GTYRAC	1 cut(s)	1619
HindII	GTYRAC	1 cut(s)	1619
HindIII	AAGCTT	5 cut(s)	838, 855, 882, 1420, 2252
HpaII	CCGG	6 cut(s)	90, 503, 575, 696, 1052, 1538
HphI	GGTGA	6 cut(s)	485, 736, 1391, 1663, 1763, 1775
Hpy166II	GTNNAC	1 cut(s)	1619
Hpy188I	TCNGA	9 cut(s)	22, 301, 897, 1315, 1464, 1572, 1684, 1717, 1999
Hpy188III	TCNNGA	8 cut(s)	193, 316, 536, 623, 1154, 1736, 1818, 2222
Hpy8I	GTNNAC	1 cut(s)	1619
Hpy99I	CGWCG	2 cut(s)	45, 194
HpyCH4III	ACNGT	3 cut(s)	1218, 1546, 1969
HpyCH4IV	ACGT	4 cut(s)	1159, 1240, 2067, 2099
HpyF10VI	GCNNNNNNNGC	3 cut(s)	195, 328, 1840
HpyF3I	CTNAG	2 cut(s)	896, 1463
HpySE526I	ACGT	4 cut(s)	1159, 1240, 2067, 2099
Hsp92I	GRCGYC	2 cut(s)	43, 189
Ksp22I	TGATCA	3 cut(s)	532, 1651, 1717
LguI	GCTCTTC	1 cut(s)	64
LmnI	GCTCC	5 cut(s)	4, 1625, 1863, 2093, 2138
Lsp1109I	GCAGC	5 cut(s)	62, 770, 1603, 2002, 2005
LweI	GCATC	3 cut(s)	232, 1675, 2150
MabI	ACCWGGT	1 cut(s)	1188
MaeI	CTAG	6 cut(s)	1797, 1883, 1949, 2304, 2354, 2367
MaeII	ACGT	4 cut(s)	1159, 1240, 2067, 2099
MaeIII	GTNAC	3 cut(s)	1447, 1763, 2023
MfeI	CAATTG	1 cut(s)	816
MflI	RGATCY	7 cut(s)	34, 312, 1228, 1459, 2086, 2131, 2191
MhlI	GDGCHC	2 cut(s)	76, 2278
MlsI	TGGCCA	1 cut(s)	2331
MluNI	TGGCCA	1 cut(s)	2331
MlyI	GAGTC	6 cut(s)	1741, 1760, 1961, 2068, 2158, 2173
MmeI	TCCRAC	1 cut(s)	655
Mox20I	TGGCCA	1 cut(s)	2331
MroXI	GAANNNNTTC	4 cut(s)	91, 111, 839, 1161
MscI	TGGCCA	1 cut(s)	2331
MslI	CAYNNNNRTG	1 cut(s)	540
Msp20I	TGGCCA	1 cut(s)	2331
MspA1I	CMGCKG	1 cut(s)	1201
MspCI	CTTAAG	1 cut(s)	1777
MspI	CCGG	6 cut(s)	90, 503, 575, 696, 1052, 1538
MspR9I	CCNGG	9 cut(s)	696, 792, 1052, 1053, 1190, 1367, 1538, 2091, 2136
MunI	CAATTG	1 cut(s)	816
Mva1269I	GAATGC	1 cut(s)	2286
MvaI	CCWGG	5 cut(s)	792, 1190, 1367, 2091, 2136
MvnI	CGCG	1 cut(s)	150
MwoI	GCNNNNNNNGC	3 cut(s)	195, 328, 1840
NciI	CCSGG	4 cut(s)	696, 1052, 1053, 1538
NdeI	CATATG	1 cut(s)	2317
NheI	GCTAGC	1 cut(s)	2353
NmeAIII	GCCGAG	1 cut(s)	1892
NmuCI	GTSAC	1 cut(s)	1763
NspI	RCATGY	3 cut(s)	1049, 1152, 1598
NspV	TTCGAA	2 cut(s)	278, 344
PagI	TCATGA	2 cut(s)	535, 2221
PceI	AGGCCT	1 cut(s)	400
PciSI	GCTCTTC	1 cut(s)	64
PctI	GAATGC	1 cut(s)	2286
PdmI	GAANNNNTTC	4 cut(s)	91, 111, 839, 1161
PfeI	GAWTC	5 cut(s)	112, 275, 1121, 1139, 2000
PflMI	CCANNNNNTGG	1 cut(s)	2215
PfoI	TCCNGGA	4 cut(s)	1365, 1536, 2089, 2134
PinAI	ACCGGT	1 cut(s)	89
PkrI	GCNGC	6 cut(s)	52, 188, 760, 1593, 2017, 2020
PleI	GAGTC	6 cut(s)	1740, 1760, 1960, 2068, 2158, 2173
PmaCI	CACGTG	1 cut(s)	1241
PmlI	CACGTG	1 cut(s)	1241
PpsI	GAGTC	6 cut(s)	1740, 1760, 1960, 2068, 2158, 2173
Ppu21I	YACGTR	2 cut(s)	1241, 2068
PpuMI	RGGWCCY	3 cut(s)	706, 1024, 1886
Psp124BI	GAGCTC	1 cut(s)	76
Psp5II	RGGWCCY	3 cut(s)	706, 1024, 1886
Psp6I	CCWGG	5 cut(s)	790, 1188, 1365, 2089, 2134
PspCI	CACGTG	1 cut(s)	1241
PspFI	CCCAGC	1 cut(s)	1201
PspGI	CCWGG	5 cut(s)	790, 1188, 1365, 2089, 2134
PspOMI	GGGCCC	1 cut(s)	2274
PspPI	GGNCC	7 cut(s)	22, 125, 706, 1024, 1886, 2274, 2275
PspPPI	RGGWCCY	3 cut(s)	706, 1024, 1886
PstI	CTGCAG	2 cut(s)	915, 2023
PsuI	RGATCY	7 cut(s)	34, 312, 1228, 1459, 2086, 2131, 2191
PvuII	CAGCTG	1 cut(s)	1201
RsaI	GTAC	1 cut(s)	1215
RsaNI	GTAC	1 cut(s)	1214
RseI	CAYNNNNRTG	1 cut(s)	540
SacI	GAGCTC	1 cut(s)	76
SapI	GCTCTTC	1 cut(s)	64
SatI	GCNGC	6 cut(s)	51, 187, 759, 1592, 2016, 2019
Sau96I	GGNCC	7 cut(s)	22, 125, 706, 1024, 1886, 2274, 2275
SchI	GAGTC	6 cut(s)	1741, 1760, 1961, 2068, 2158, 2173
ScrFI	CCNGG	9 cut(s)	696, 792, 1052, 1053, 1190, 1367, 1538, 2091, 2136
SduI	GDGCHC	2 cut(s)	76, 2278
SexAI	ACCWGGT	1 cut(s)	1188
SfaNI	GCATC	3 cut(s)	232, 1675, 2150
SfcI	CTRYAG	4 cut(s)	432, 911, 1900, 2019
SfuI	TTCGAA	2 cut(s)	278, 344
SinI	GGWCC	5 cut(s)	22, 125, 706, 1024, 1886
SmaI	CCCGGG	1 cut(s)	1053
SmiMI	CAYNNNNRTG	1 cut(s)	540
SmlI	CTYRAG	1 cut(s)	1777
SmoI	CTYRAG	1 cut(s)	1777
SpeI	ACTAGT	1 cut(s)	2366
SseBI	AGGCCT	1 cut(s)	400
SsiI	CCGC	9 cut(s)	150, 186, 245, 283, 322, 371, 387, 546, 1520
SspMI	CTAG	6 cut(s)	1797, 1883, 1949, 2304, 2354, 2367
SstI	GAGCTC	1 cut(s)	76
StuI	AGGCCT	1 cut(s)	400
StyD4I	CCNGG	9 cut(s)	694, 790, 1050, 1051, 1188, 1365, 1536, 2089, 2134
StyI	CCWWGG	3 cut(s)	170, 1085, 1692
TaaI	ACNGT	3 cut(s)	1218, 1546, 1969
TaiI	ACGT	4 cut(s)	1162, 1243, 2070, 2102
TatI	WGTACW	1 cut(s)	1213
TauI	GCSGC	1 cut(s)	189
TfiI	GAWTC	5 cut(s)	112, 275, 1121, 1139, 2000
TscAI	CASTG	2 cut(s)	1778, 2347
TseFI	GTSAC	1 cut(s)	1763
TseI	GCWGC	5 cut(s)	50, 758, 1591, 2015, 2018
Tsp45I	GTSAC	1 cut(s)	1763
TspGWI	ACGGA	3 cut(s)	683, 1466, 1868
TspMI	CCCGGG	1 cut(s)	1051
TspRI	CASTG	2 cut(s)	1778, 2347
Van91I	CCANNNNNTGG	1 cut(s)	2215
Vha464I	CTTAAG	1 cut(s)	1777
VpaK11BI	GGWCC	5 cut(s)	22, 125, 706, 1024, 1886
XapI	RAATTY	3 cut(s)	295, 340, 1361
XceI	RCATGY	3 cut(s)	1049, 1152, 1598
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1373, 2339
XmaI	CCCGGG	1 cut(s)	1051
XmnI	GAANNNNTTC	4 cut(s)	91, 111, 839, 1161
XspI	CTAG	6 cut(s)	1797, 1883, 1949, 2304, 2354, 2367

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.