Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Reverse (-)

38423283 .. 38431921

Gene Structure 8639 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG215000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3144 bp

ATGCCCCGTCACAGAGACGAAGACGACGACGAGATGGAGCCGGACGAGGACGAGTACGACCCGGACCAAGGTCTAGACGACGTCGACGACGAAGAGGAGGAGGAGGAGGAGGAGGACCGAGGAGGCCGGAGGTCCAGTCAGAAGCGGAGGAGATCGAGCTTCATTGACGATGTGGCCGAGGAGGACGACGAGGAGGAAGAGGAGGACTACGAGGACGAGGATTACGGCGCCTACCGTGGCGGTGGCGGTGGCGGTGGGAGTCGTAAGAAGAGCAAAAAGAAGCCCTCCGGTGCGGATTTTCTTGATCTCGAAGCGGTAGTGGATTCGGATGAGGAGGAAGAGGAGGATGAGGGCGAGGACGACTTTATTGATTCCGGAGCTGATATACCTGAGGAGGGCGATGGTAGAAGGATGCATCATCGCCCATTGATGGATGACCAAGAGCAAGAAGATGTCGAGGCTCTGGAAAGAAGAATTCAGGCAAGATATGCAAAGCAACATCATACAGAAGATACTGAGGAGACCACTGATGTGGATCAGCAAGCCCTTTTACCTTCTGTTTTGGACCCAAAGCTGTGGATGGTGAAATGTGCGATTGGTAAAGAACGAGAGGTAGCTGCTTGCTTAATGCAAAAGTATATTGATAAACCTGAATTGAACATCAGGTCTGTTATTGCTCTTGACCACCTGAAGAACTATATATACGTTGAAGCGGAAAAAGAAGCCCACGTGAGGGAGGCTTGCAAAGGATTGCGCAATATATTTACCCAGAAAATAGCACTTGTTCCTATTAGAGAAATGACTGATGTTCTTTCCGTTGAAAGCAAGGCAATTGATATTTCTAGGGATACTTGGGTCAGAATGAAGATTGGGATATATAAAGGGGACCTTGCCAAGGTTGTTGATGTGGATAATGTACGACAGAGAGTTACAGTAAAATTAATTCCAAGAATTGATTTACAAGCTCTTGCAAATAAACTGGAGGGCAGAGAAGTTGTGAAAAAGAAAGCGTTTGTGCCTCCGCCACGTTTTATGAATATTGATGAAGCTAGAGAACTGCATATTCGTGTGGAGCGTAAACGAGATCCAATGACGGGTGACTACTTTGAAAACATTGAGAAAATGATGTTTAAAGATGGTTTCTTGTATAAAATAGTATCAATGAAATCAATTAGTACTCAAAACATACATCCCACTTTTGATGAACTGGAAAAGTTTCGGAAACCTGGGGAGAATGGGGAAGGAGATGTTGCCAGTCTGTCAACTTTATTTTCAAACAGAAAGAAAGGACACTTTGTGAAGGGTGATGCAGTCATTGTCATCAAGGGAGACCTAAAAAACTTGAAAGGGCGGGTTGAGAAAGTTGAGGAAGGGACTGTGCATATCAGACCCGACATGAAAGACCTCCCAAAAACTCTTGCCATAAATGAAAAAGAACTTTGCAAGTACTTTGAACCTGGGAATCATGTGAAGGTTGTATCTGGTACTCAAGAAGGTGCAACTGGTATGGTTGTCAAGGTCGATCAGCATGTGCTCATCATTTTATCTGATACAACTAAGGAACATCTCCGTGTGTTTGCTGATGATGTTGTGGAGAGTTCTGAGGTGACATCTGGTATTACCAAAATTGGGGACTATGAGCTTCATGATCTTGTGCTACTGGATAATAATAGCTTTGGTGTAATTATACGTGTTGAAACTGAAGCATGTCAGGTCCTTAAGGGAGTTCCTGAGAGACCTGAGGTTGCCCTTGTCAAATTAAGAGAGATCAAATGCAAGATTGAGAAAAAGCTTAGTGTGCAAGATTGTTTTAAGAACACAATATCTGTGAAAGATGTTGTGAGGGTCGTTGAAGGTCCTTCCAAGGGTAAACAAGGTCCCGTGGAACACATATATAGAGGAGTCTTATTCATTTATGATCGCCATCACATAGAGCATGCGGGCTTTATCTGTGTTAAGTCACATTCTTGTCGTGTTGTAGGAGGATCACGTGCCAATGGTGATAGAAATGGTGACTCCTACTCTAGATTTGATCATCTTCGAGCTCCTCCTCTTATTCCCCAGTCCCCCAGGAGGTTTCCTAGAGGAGGCCCTCCTAATAACTTTGGGGGAAGAAATAGAGGTGGAAGAGGGCATGATGGATTGGTTGGGACTACAGTAAAGATTCGCCAGGGTGCCTATAAGGGTTACCGTGGGCGCGTTGTTGAGGTTAAGGGAACTAGTGTACGGGTGGAACTTGAGTCCCAAATGAAAGTTGTGACAGTTGACCGGACTTGTATTTCTGATAACGTGGCTATTACAACACCGTATCGTGATACATCTAGCTATGGTATGGGAAGTCAGACCCCAATTCATCCTTCTCGAACTCCGCTCCATCCATATATGACGCCAATGAGGGATGCTGGAGCAACGCCTATTCATGATGGCATGAGGACTCCAATGCGTGACCGAGCATGGAATCCGTATGCACCTATGAGTCCAGCAAGGGATAGCTGGGAGGAAGGAAACCCTGGTTCTTGGGGTTCTAGCCCACAGTATCAGCCAGGAAGTCCTCCCTCACGGACGTACGAAGCTCCAACTCCTGGTTCAGGTTGGGCCAACACTCCTGGTGGGAATTATAGTGAAGCTGGAACACCAAGGGACAGTACTGGTTATGCAAATGCTCCTAGCCCATACTTGCCGTCAACTCCCGGTGGGCAGCCTATGACACCAAACTCAGCATCCTATCTTCCCGGAACTCCTGGAGGGCAGCCAATGACACCAGGGACTGGTGGTCTGGATATGATGTCTCCTGTTATAGGAGGGGACAATGAAGGACCATGGTTCATGCCAGATATACTGGTCAATGTACGCAATTCTGGAGAGGAAACTACTGGAATTGTCAGAGAAGTGCTCCTGGATGGCTCATGTAGGGTAGCTCTTGGCTCGGGTGGCAATGGTGATACTGTAACTGTACTTCCAAATGAAATGGAGGTAGTCGTACCAAGGAAGAATGATAAGATCAAAATCATGGGTGGCTCATTACGCGGTGCCACTGGCAAGCTGATTGGTGTCGATGGCACGGATGGAATTGTCAAGGTTGATGACACTCTTGATGTTAAAATATTAGACTTGGCTATTTTGTCAAAACTAGGTCAATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

1047

Amino Acids

116.15

Weight (kDa)

5.23

Isoelectric Point (pI)

42.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5_N	PF11942	100 - 185	3.6e-12	Spt5 transcription elongation factor, acidic N-terminal
Spt5-NGN	PF03439	191 - 271	8.2e-27	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	277 - 406	5e-76	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	434 - 483	4.1e-22	Transcription elongation factor SPT5, second KOW domain
KOWx_SPT5	PF23037	542 - 593	1.4e-17	Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5	PF23291	601 - 654	7e-24	Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5	PF23290	715 - 758	1.5e-17	Transcription elongation factor SPT5, fifth KOW domain
KOW6_SPT51-2	PF23038	942 - 991	2.4e-26	Transcription elongation factor SPT5 homolog 1/2, KOW6 domain
KOW7_SPT5	PF23287	993 - 1044	3.8e-21	Transcription elongation factor SPT5, seventh KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	84
Acc16I	TGCGCA	1 cut(s)	755
AccB1I	GGYRCC	3 cut(s)	227, 2174, 3029
AccB7I	CCANNNNNTGG	2 cut(s)	2582, 2768
AccBSI	CCGCTC	1 cut(s)	2371
AccI	GTMKAC	1 cut(s)	84
AccII	CGCG	2 cut(s)	2199, 3027
AccIII	TCCGGA	1 cut(s)	374
AclWI	GGATC	3 cut(s)	543, 1079, 1993
AcoI	YGGCCR	1 cut(s)	174
AcsI	RAATTY	1 cut(s)	474
AcuI	CTGAAG	2 cut(s)	710, 1722
AcvI	CACGTG	2 cut(s)	730, 1991
AcyI	GRCGYC	3 cut(s)	81, 228, 2387
AdeI	CACNNNGTG	1 cut(s)	1297
AflII	CTTAAG	1 cut(s)	1718
AflIII	ACRYGT	1 cut(s)	1690
AgsI	TTSAA	9 cut(s)	658, 710, 821, 1109, 1275, 1345, 1454, 1697, 1853
AhdI	GACNNNNNGTC	1 cut(s)	2772
AhlI	ACTAGT	1 cut(s)	2219
AjuI	GAANNNNNNNTTGG	4 cut(s)	2341, 2373, 2569, 2601
AleI	CACNNNNGTG	1 cut(s)	530
Alw21I	GWGCWC	3 cut(s)	1536, 2047, 2895
Alw26I	GTCTC	5 cut(s)	9, 515, 1323, 1729, 2793
AlwI	GGATC	3 cut(s)	543, 1079, 1993
AlwNI	CAGNNNCTG	1 cut(s)	2768
Ama87I	CYCGRG	1 cut(s)	2926
Aor13HI	TCCGGA	1 cut(s)	374
AoxI	GGCC	4 cut(s)	124, 174, 2089, 2595
ApeKI	GCWGC	3 cut(s)	617, 2698, 2749
ApoI	RAATTY	1 cut(s)	474
AseI	ATTAAT	1 cut(s)	941
Asp700I	GAANNNNTTC	1 cut(s)	1215
AspLEI	GCGC	3 cut(s)	230, 756, 2199
AsuC2I	CCSGG	3 cut(s)	62, 2691, 2733
AsuHPI	GGTGA	7 cut(s)	595, 1109, 1316, 1618, 2012, 2024, 2951
AvaI	CYCGRG	1 cut(s)	2926
AvaII	GGWCC	9 cut(s)	64, 115, 132, 565, 886, 1714, 1856, 1877, 2816
AxyI	CCTNAGG	2 cut(s)	390, 1740
BaeI	ACNNNNGTAYC	2 cut(s)	840, 873
BanI	GGYRCC	3 cut(s)	227, 2174, 3029
BanII	GRGCYC	1 cut(s)	2047
BarI	GAAGNNNNNNTAC	2 cut(s)	2940, 2972
BbrPI	CACGTG	2 cut(s)	730, 1991
BbsI	GAAGAC	1 cut(s)	27
Bbv12I	GWGCWC	3 cut(s)	1536, 2047, 2895
BbvI	GCAGC	3 cut(s)	604, 2710, 2761
BceAI	ACGGC	2 cut(s)	241, 2665
BciVI	GTATCC	1 cut(s)	841
BclI	TGATCA	1 cut(s)	2032
BcnI	CCSGG	3 cut(s)	62, 2691, 2733
BcoDI	GTCTC	5 cut(s)	9, 515, 1323, 1729, 2793
BcuI	ACTAGT	1 cut(s)	2219
BfmI	CTRYAG	1 cut(s)	2154
BfoI	RGCGCY	1 cut(s)	231
BfrI	CTTAAG	1 cut(s)	1718
BfuI	GTATCC	1 cut(s)	841
BisI	GCNGC	3 cut(s)	618, 2699, 2750
BlsI	GCNGC	3 cut(s)	619, 2700, 2751
BmcAI	AGTACT	3 cut(s)	1177, 1448, 2647
Bme18I	GGWCC	9 cut(s)	64, 115, 132, 565, 886, 1714, 1856, 1877, 2816
BmeRI	GACNNNNNGTC	1 cut(s)	2772
BmeT110I	CYCGRG	1 cut(s)	2926
BmiI	GGNNCC	7 cut(s)	39, 229, 567, 887, 1879, 2176, 3031
BmrI	ACTGGG	1 cut(s)	2056
BmsI	GCATC	5 cut(s)	402, 424, 1297, 2389, 2729
BmuI	ACTGGG	1 cut(s)	2056
BoxI	GACNNNNGTC	1 cut(s)	69
BpiI	GAAGAC	1 cut(s)	27
BplI	GAGNNNNNCTC	2 cut(s)	2877, 2909
BpmI	CTGGAG	4 cut(s)	1001, 2424, 2763, 2880
BpuEI	CTTGAG	2 cut(s)	1473, 2258
BpuMI	CCSGG	3 cut(s)	62, 2691, 2733
BsaAI	YACGTR	3 cut(s)	730, 1691, 1991
BsaBI	GATNNNNATC	3 cut(s)	534, 1923, 3005
BsaHI	GRCGYC	3 cut(s)	81, 228, 2387
BsaI	GGTCTC	3 cut(s)	515, 1323, 1729
BsaWI	WCCGGW	3 cut(s)	287, 374, 2268
BsaXI	ACNNNNNCTCC	2 cut(s)	121, 151
Bse21I	CCTNAGG	2 cut(s)	390, 1740
Bse3DI	GCAATG	1 cut(s)	2941
Bse8I	GATNNNNATC	3 cut(s)	534, 1923, 3005
BseAI	TCCGGA	1 cut(s)	374
BseJI	GATNNNNATC	3 cut(s)	534, 1923, 3005
BseMI	GCAATG	1 cut(s)	2941
BseMII	CTCAG	6 cut(s)	381, 507, 1593, 1722, 1731, 2730
BseXI	GCAGC	3 cut(s)	604, 2710, 2761
BseYI	CCCAGC	1 cut(s)	2493
Bsh1236I	CGCG	2 cut(s)	2199, 3027
BshFI	GGCC	4 cut(s)	126, 176, 2091, 2597
BshNI	GGYRCC	3 cut(s)	227, 2174, 3029
BsiHKAI	GWGCWC	3 cut(s)	1536, 2047, 2895
BsiHKCI	CYCGRG	1 cut(s)	2926
BsiSI	CCGG	8 cut(s)	41, 62, 127, 288, 375, 2269, 2691, 2733
BsiWI	CGTACG	1 cut(s)	2565
BsmAI	GTCTC	5 cut(s)	9, 515, 1323, 1729, 2793
BsmBI	CGTCTC	1 cut(s)	9
BsnI	GGCC	4 cut(s)	126, 176, 2091, 2597
Bso31I	GGTCTC	3 cut(s)	515, 1323, 1729
BsoBI	CYCGRG	1 cut(s)	2926
Bsp1286I	GDGCHC	3 cut(s)	1536, 2047, 2895
Bsp13I	TCCGGA	1 cut(s)	374
Bsp19I	CCATGG	1 cut(s)	2819
BspANI	GGCC	4 cut(s)	126, 176, 2091, 2597
BspCNI	CTCAG	6 cut(s)	382, 508, 1594, 1723, 1732, 2729
BspEI	TCCGGA	1 cut(s)	374
BspFNI	CGCG	2 cut(s)	2199, 3027
BspHI	TCATGA	2 cut(s)	1645, 2419
BspLI	GGNNCC	7 cut(s)	39, 229, 567, 887, 1879, 2176, 3031
BspPI	GGATC	3 cut(s)	543, 1079, 1993
BspQI	GCTCTTC	1 cut(s)	263
BspT107I	GGYRCC	3 cut(s)	227, 2174, 3029
BspTI	CTTAAG	1 cut(s)	1718
BspTNI	GGTCTC	3 cut(s)	515, 1323, 1729
BsrBI	CCGCTC	1 cut(s)	2371
BsrDI	GCAATG	1 cut(s)	2941
BssNI	GRCGYC	3 cut(s)	81, 228, 2387
BssT1I	CCWWGG	6 cut(s)	67, 894, 1863, 2636, 2819, 2984
Bst6I	CTCTTC	5 cut(s)	87, 192, 263, 333, 2122
BstACI	GRCGYC	3 cut(s)	81, 228, 2387
BstAFI	CTTAAG	1 cut(s)	1718
BstAPI	GCANNNNNTGC	1 cut(s)	488
BstBAI	YACGTR	3 cut(s)	730, 1691, 1991
BstC8I	GCNNGC	6 cut(s)	543, 622, 742, 1938, 1942, 3041
BstDEI	CTNAG	8 cut(s)	390, 516, 1557, 1602, 1731, 1740, 1793, 2716
BstDSI	CCRYGG	4 cut(s)	235, 1881, 2191, 2819
BstEII	GGTNACC	1 cut(s)	2186
BstENI	CCTNNNNNAGG	4 cut(s)	893, 2085, 2586, 2796
BstFNI	CGCG	2 cut(s)	2199, 3027
BstH2I	RGCGCY	1 cut(s)	231
BstHHI	GCGC	3 cut(s)	230, 756, 2199
BstMAI	GTCTC	5 cut(s)	9, 515, 1323, 1729, 2793
BstMWI	GCNNNNNNNGC	4 cut(s)	488, 1798, 2931, 3024
BstNSI	RCATGY	3 cut(s)	1532, 1710, 1940
BstPAI	GACNNNNGTC	1 cut(s)	69
BstPI	GGTNACC	1 cut(s)	2186
BstSFI	CTRYAG	1 cut(s)	2154
BstUI	CGCG	2 cut(s)	2199, 3027
BstV1I	GCAGC	3 cut(s)	604, 2710, 2761
BstV2I	GAAGAC	1 cut(s)	27
BstX2I	RGATCY	1 cut(s)	1084
BstXI	CCANNNNNNTGG	2 cut(s)	532, 576
BstYI	RGATCY	1 cut(s)	1084
Bsu36I	CCTNAGG	2 cut(s)	390, 1740
BsuI	GTATCC	1 cut(s)	841
BsuRI	GGCC	4 cut(s)	126, 176, 2091, 2597
BtgI	CCRYGG	4 cut(s)	235, 1881, 2191, 2819
BtgZI	GCGATG	2 cut(s)	404, 414
BtsIMutI	CAGTG	2 cut(s)	525, 3033
Cac8I	GCNNGC	6 cut(s)	543, 622, 742, 1938, 1942, 3041
CaiI	CAGNNNCTG	1 cut(s)	2768
CciI	TCATGA	2 cut(s)	1645, 2419
CfoI	GCGC	3 cut(s)	230, 756, 2199
CseI	GACGC	1 cut(s)	2395
DdeI	CTNAG	8 cut(s)	390, 516, 1557, 1602, 1731, 1740, 1793, 2716
DinI	GGCGCC	1 cut(s)	229
DraI	TTTAAA	1 cut(s)	1132
DraIII	CACNNNGTG	1 cut(s)	1297
DriI	GACNNNNNGTC	1 cut(s)	2772
EaeI	YGGCCR	1 cut(s)	174
Eam1104I	CTCTTC	5 cut(s)	87, 192, 263, 333, 2122
Eam1105I	GACNNNNNGTC	1 cut(s)	2772
EarI	CTCTTC	5 cut(s)	87, 192, 263, 333, 2122
EciI	GGCGGA	1 cut(s)	1011
Ecl136II	GAGCTC	1 cut(s)	2045
Eco130I	CCWWGG	6 cut(s)	67, 894, 1863, 2636, 2819, 2984
Eco24I	GRGCYC	1 cut(s)	2047
Eco31I	GGTCTC	3 cut(s)	515, 1323, 1729
Eco47I	GGWCC	9 cut(s)	64, 115, 132, 565, 886, 1714, 1856, 1877, 2816
Eco53kI	GAGCTC	1 cut(s)	2045
Eco57I	CTGAAG	2 cut(s)	710, 1722
Eco72I	CACGTG	2 cut(s)	730, 1991
Eco81I	CCTNAGG	2 cut(s)	390, 1740
Eco88I	CYCGRG	1 cut(s)	2926
Eco91I	GGTNACC	1 cut(s)	2186
EcoICRI	GAGCTC	1 cut(s)	2045
EcoNI	CCTNNNNNAGG	4 cut(s)	893, 2085, 2586, 2796
EcoO109I	RGGNCCY	5 cut(s)	886, 1714, 1856, 1877, 2090
EcoO65I	GGTNACC	1 cut(s)	2186
EcoRI	GAATTC	1 cut(s)	474
EcoT14I	CCWWGG	6 cut(s)	67, 894, 1863, 2636, 2819, 2984
EcoT22I	ATGCAT	1 cut(s)	417
EcoT38I	GRGCYC	1 cut(s)	2047
EgeI	GGCGCC	1 cut(s)	229
EheI	GGCGCC	1 cut(s)	229
ErhI	CCWWGG	6 cut(s)	67, 894, 1863, 2636, 2819, 2984
Esp3I	CGTCTC	1 cut(s)	9
FalI	AAGNNNNNCTT	2 cut(s)	873, 905
FauI	CCCGC	2 cut(s)	1344, 1933
FbaI	TGATCA	1 cut(s)	2032
FblI	GTMKAC	1 cut(s)	84
Fnu4HI	GCNGC	3 cut(s)	618, 2699, 2750
FriOI	GRGCYC	1 cut(s)	2047
Fsp4HI	GCNGC	3 cut(s)	618, 2699, 2750
FspI	TGCGCA	1 cut(s)	755
GlaI	GCGC	3 cut(s)	229, 755, 2198
GluI	GCNGC	3 cut(s)	618, 2699, 2750
GsaI	CCCAGC	1 cut(s)	2497
GsuI	CTGGAG	4 cut(s)	1001, 2424, 2763, 2880
HaeII	RGCGCY	1 cut(s)	231
HaeIII	GGCC	4 cut(s)	126, 176, 2091, 2597
HapII	CCGG	8 cut(s)	41, 62, 127, 288, 375, 2269, 2691, 2733
HgaI	GACGC	1 cut(s)	2395
HhaI	GCGC	3 cut(s)	230, 756, 2199
Hin1I	GRCGYC	3 cut(s)	81, 228, 2387
Hin6I	GCGC	3 cut(s)	228, 754, 2197
HinP1I	GCGC	3 cut(s)	228, 754, 2197
HincII	GTYRAC	4 cut(s)	85, 1263, 2266, 2685
HindII	GTYRAC	4 cut(s)	85, 1263, 2266, 2685
HindIII	AAGCTT	1 cut(s)	1790
HpaII	CCGG	8 cut(s)	41, 62, 127, 288, 375, 2269, 2691, 2733
HphI	GGTGA	7 cut(s)	595, 1109, 1316, 1618, 2012, 2024, 2951
Hpy166II	GTNNAC	7 cut(s)	85, 1079, 1263, 1871, 2225, 2266, 2685
Hpy8I	GTNNAC	7 cut(s)	85, 1079, 1263, 1871, 2225, 2266, 2685
Hpy99I	CGWCG	7 cut(s)	29, 32, 83, 86, 89, 92, 191
HpyCH4IV	ACGT	8 cut(s)	81, 705, 729, 1027, 1690, 1990, 2289, 2564
HpyF10VI	GCNNNNNNNGC	4 cut(s)	488, 1798, 2931, 3024
HpyF3I	CTNAG	8 cut(s)	390, 516, 1557, 1602, 1731, 1740, 1793, 2716
HpySE526I	ACGT	8 cut(s)	81, 705, 729, 1027, 1690, 1990, 2289, 2564
Hsp92I	GRCGYC	3 cut(s)	81, 228, 2387
HspAI	GCGC	3 cut(s)	228, 754, 2197
KasI	GGCGCC	1 cut(s)	227
Kpn2I	TCCGGA	1 cut(s)	374
Ksp22I	TGATCA	1 cut(s)	2032
LguI	GCTCTTC	1 cut(s)	263
LmnI	GCTCC	9 cut(s)	37, 377, 1072, 2050, 2376, 2405, 2578, 2668, 2898
Lsp1109I	GCAGC	3 cut(s)	604, 2710, 2761
LweI	GCATC	5 cut(s)	402, 424, 1297, 2389, 2729
MaeII	ACGT	8 cut(s)	81, 705, 729, 1027, 1690, 1990, 2289, 2564
MbiI	CCGCTC	1 cut(s)	2371
MfeI	CAATTG	1 cut(s)	831
MflI	RGATCY	1 cut(s)	1084
MhlI	GDGCHC	3 cut(s)	1536, 2047, 2895
Mly113I	GGCGCC	1 cut(s)	228
MlyI	GAGTC	6 cut(s)	268, 1911, 2009, 2249, 2428, 2485
MmeI	TCCRAC	1 cut(s)	2600
Mph1103I	ATGCAT	1 cut(s)	417
MroI	TCCGGA	1 cut(s)	374
MroXI	GAANNNNTTC	1 cut(s)	1215
MseI	TTAA	9 cut(s)	626, 941, 1131, 1719, 1760, 1812, 1956, 2211, 3099
MslI	CAYNNNNRTG	3 cut(s)	530, 1065, 1569
MspCI	CTTAAG	1 cut(s)	1718
MspI	CCGG	8 cut(s)	41, 62, 127, 288, 375, 2269, 2691, 2733
MunI	CAATTG	1 cut(s)	831
MvnI	CGCG	2 cut(s)	2199, 3027
MwoI	GCNNNNNNNGC	4 cut(s)	488, 1798, 2931, 3024
NarI	GGCGCC	1 cut(s)	228
NciI	CCSGG	3 cut(s)	62, 2691, 2733
NcoI	CCATGG	1 cut(s)	2819
NlaIV	GGNNCC	7 cut(s)	39, 229, 567, 887, 1879, 2176, 3031
NmeAIII	GCCGAG	1 cut(s)	202
NmuCI	GTSAC	7 cut(s)	8, 1097, 1606, 1959, 2012, 2257, 2444
NsbI	TGCGCA	1 cut(s)	755
NsiI	ATGCAT	1 cut(s)	417
NspI	RCATGY	3 cut(s)	1532, 1710, 1940
OliI	CACNNNNGTG	1 cut(s)	530
PaeI	GCATGC	1 cut(s)	1940
PagI	TCATGA	2 cut(s)	1645, 2419
PciSI	GCTCTTC	1 cut(s)	263
PcsI	WCGNNNNNNNCGW	5 cut(s)	24, 84, 87, 174, 1072
PdmI	GAANNNNTTC	1 cut(s)	1215
PfeI	GAWTC	5 cut(s)	323, 371, 1462, 2164, 2458
Pfl23II	CGTACG	1 cut(s)	2565
PflFI	GACNNNGTC	1 cut(s)	80
PflMI	CCANNNNNTGG	2 cut(s)	2582, 2768
PfoI	TCCNGGA	3 cut(s)	2731, 2740, 2895
PkrI	GCNGC	3 cut(s)	619, 2700, 2751
PleI	GAGTC	6 cut(s)	267, 1910, 2009, 2248, 2428, 2484
PluTI	GGCGCC	1 cut(s)	231
PmaCI	CACGTG	2 cut(s)	730, 1991
PmlI	CACGTG	2 cut(s)	730, 1991
PpsI	GAGTC	6 cut(s)	267, 1910, 2009, 2248, 2428, 2484
Ppu21I	YACGTR	3 cut(s)	730, 1691, 1991
PpuMI	RGGWCCY	4 cut(s)	886, 1714, 1856, 1877
PshAI	GACNNNNGTC	1 cut(s)	69
PshBI	ATTAAT	1 cut(s)	941
Psp124BI	GAGCTC	1 cut(s)	2047
Psp5II	RGGWCCY	4 cut(s)	886, 1714, 1856, 1877
PspCI	CACGTG	2 cut(s)	730, 1991
PspEI	GGTNACC	1 cut(s)	2186
PspFI	CCCAGC	1 cut(s)	2493
PspLI	CGTACG	1 cut(s)	2565
PspN4I	GGNNCC	7 cut(s)	39, 229, 567, 887, 1879, 2176, 3031
PspPPI	RGGWCCY	4 cut(s)	886, 1714, 1856, 1877
PsrI	GAACNNNNNNTAC	2 cut(s)	686, 718
PstNI	CAGNNNCTG	1 cut(s)	2768
PsuI	RGATCY	1 cut(s)	1084
PsyI	GACNNNGTC	1 cut(s)	80
RseI	CAYNNNNRTG	3 cut(s)	530, 1065, 1569
SacI	GAGCTC	1 cut(s)	2047
SalI	GTCGAC	1 cut(s)	83
SapI	GCTCTTC	1 cut(s)	263
SaqAI	TTAA	9 cut(s)	626, 941, 1131, 1719, 1760, 1812, 1956, 2211, 3099
SatI	GCNGC	3 cut(s)	618, 2699, 2750
ScaI	AGTACT	3 cut(s)	1177, 1448, 2647
SchI	GAGTC	6 cut(s)	268, 1911, 2009, 2249, 2428, 2485
SduI	GDGCHC	3 cut(s)	1536, 2047, 2895
SfaNI	GCATC	5 cut(s)	402, 424, 1297, 2389, 2729
SfcI	CTRYAG	1 cut(s)	2154
SfoI	GGCGCC	1 cut(s)	229
SgrDI	CGTCGACG	1 cut(s)	83
SinI	GGWCC	9 cut(s)	64, 115, 132, 565, 886, 1714, 1856, 1877, 2816
SmiMI	CAYNNNNRTG	3 cut(s)	530, 1065, 1569
SmlI	CTYRAG	3 cut(s)	1488, 1718, 2237
SmoI	CTYRAG	3 cut(s)	1488, 1718, 2237
SpeI	ACTAGT	1 cut(s)	2219
SphI	GCATGC	1 cut(s)	1940
SspDI	GGCGCC	1 cut(s)	227
SspI	AATATT	2 cut(s)	1039, 3105
SstI	GAGCTC	1 cut(s)	2047
StyI	CCWWGG	6 cut(s)	67, 894, 1863, 2636, 2819, 2984
TaiI	ACGT	8 cut(s)	84, 708, 732, 1030, 1693, 1993, 2292, 2567
TaqI	TCGA	8 cut(s)	84, 155, 309, 456, 1521, 2041, 2362, 3054
TaqII	GACCGA	2 cut(s)	132, 2463
TatI	WGTACW	4 cut(s)	1175, 1446, 2645, 2953
TfiI	GAWTC	5 cut(s)	323, 371, 1462, 2164, 2458
Tru1I	TTAA	9 cut(s)	626, 941, 1131, 1719, 1760, 1812, 1956, 2211, 3099
Tru9I	TTAA	9 cut(s)	626, 941, 1131, 1719, 1760, 1812, 1956, 2211, 3099
TscAI	CASTG	2 cut(s)	532, 3040
TseFI	GTSAC	7 cut(s)	8, 1097, 1606, 1959, 2012, 2257, 2444
TseI	GCWGC	3 cut(s)	617, 2698, 2749
Tsp45I	GTSAC	7 cut(s)	8, 1097, 1606, 1959, 2012, 2257, 2444
TspGWI	ACGGA	5 cut(s)	805, 1559, 2451, 2575, 3077
TspRI	CASTG	2 cut(s)	532, 3040
Tth111I	GACNNNGTC	1 cut(s)	80
Van91I	CCANNNNNTGG	2 cut(s)	2582, 2768
Vha464I	CTTAAG	1 cut(s)	1718
VpaK11BI	GGWCC	9 cut(s)	64, 115, 132, 565, 886, 1714, 1856, 1877, 2816
VspI	ATTAAT	1 cut(s)	941
XagI	CCTNNNNNAGG	4 cut(s)	893, 2085, 2586, 2796
XapI	RAATTY	1 cut(s)	474
XbaI	TCTAGA	2 cut(s)	73, 2024
XceI	RCATGY	3 cut(s)	1532, 1710, 1940
XmiI	GTMKAC	1 cut(s)	84
XmnI	GAANNNNTTC	1 cut(s)	1215
ZraI	GACGTC	1 cut(s)	82
ZrmI	AGTACT	3 cut(s)	1177, 1448, 2647
Zsp2I	ATGCAT	1 cut(s)	417

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.