Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Reverse (-)

18177814 .. 18179823

Gene Structure 2010 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1075900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2010 bp

ATGAAGCGCCGCGGAAGAGAAAACTCGTTCTACGAACAGGAAGAAGATCCAGAAGAGGCCCTTTTTGACGACGAAGACGGAGAAGAGAAGGAGGAGGAGCGACAAGGAGGAGGCTCTGATCGTAAAAGGAGGAGATCGGATCGCAGAGAAGATTTCGCATCGAAATTTTTGGATATTGAAGCCGAGGTCGATAGCGAAGAGGAAGACTATGAATCTGAGCCCGAGGACGACGGCTTCATAGCCCAACCTGGCGACGATCCAAACCCGAGTGATGGAGAAGACGATGACGACGTTAGAAGGGCGAAACACGGTCGGCTGTTGTTCCCGGAGGAATTGGAGTCGGAGGAAGATATTGAAGAATTAGAGAGAAGAGTTCAGGGGCATTTTGATTCTCACCAAATTCAGTTTGATGATGGTGATGATGATGATGAAATTGGGCAGCAAGCTCGGTTGCCTTGCGTAAGGGACCCGAAATTGTGGATGGTCAAATGCGCGATCGGGCACGAGAGAGAGGTTGCTCTCTGCTTGGCGCAGAAGTGCATCAATAAAAAATCAAATCCATCTCATATGATCAGGTCTGCAATTGCTCTTGATAATCTGAAAAATTACATATATGTTGAGGCAGACAAAGAAGCCCATGTGAGAGAAGCTTGCAAAGGCATGCGCTATGTCTTTACTGCCCAGAAAATTGTTCTTGTTCCTATTAAGGAGATGGTAGGTGTCGTTTCTGTGGAGAGAAAACCCTCCGTTGATGTTTCGGTGGATAGTTGGGTGAGGATGAAGAGGGTAAAGATGTATGAAGGGGACCTTGGCAGGGTTGTGGATGTGGATGATGTGAGGAGGAGGGTTACGGTGAAATTGATTCCAAGGATTGATTTTCCAGAACTTGCGAGGAAATTGGAGGGACTAGGCCTACAAAAGAAGAGATTTATTCCTCCTCCTGCACGGTTGATGAACATTGATGAAGCTGAAAGAGTACTGGCGATTCAGGTAAAGGAGACAAGAGATCGGGAGACGGGTGAATACTTTGATAAGATTGGTGACATGAAGTTTAAACATGGGTTCTTGTATAAGACCGTGGCAATGAGTTCGATCAGCTGGCATAATGTAAATCCTACTTTCGATGAACTTGAAAAATTTAGAAAACCTGCAGGGAATGAGAATGCCGATCGTGGCGATGATATGTTTTCGAACCGATATAAAGGAGAGTTTATGAAGGGTGATGCAGTTGTGGTGCTGAAGGGAGATCTGATAAATTTGGCGGGGAGGGTTGAGAAGGTTGAGGGAGGGAATGTTCATATGCGACCCGACATGAAAGGCCTTCCAGAAACTCTTGTGATAAACAAGAACAATCTTTGCAAGTACTTTAAAATTGGGAATCATGTGAAGGTTACATCTGGTAGTCAGAAAGGTGCTGCTGGCATGGTTGTGAAGGTTGATCAGCAGCATGGGCTGGTCACCATTATAACTGATAAAACCAAGAAACATGTTTGCGTGTTCGCTGACGATGTTGTGGAAAGTTTTGGTGCTACTGGTGATGATGCAAATGGTGTTTCTAGTTTCTCAAGATTTGGCCATTACAGAACTCCGAGCAAGTTTTCTGGAGGTCGAAGAAGAGGGCCTGATGCTAATGGTTTGGATGCAGGGACTAGGGTGAAAATTCGGCAGGGTGTTTATAAGGGCTACCGCGGTCGTGTTGTAGAGGTCAAAGGACAAAATCTGCTGGTAGAATTAGAGTCCCAAATGAAGATTGTCACAGTTGATCGTCGAAGTTGTGTTTCTGATTCCGATAACTTTGCCGCGTTTGGAACACTGTATCGCTATGGCAATGGCATGAGAGGGACTGAGACTCCGATGAGAGATCCCAGAGCAACTCCGCTCCATTCGTTCATGACTACTCCGATGAGAGATCCTAATGCAACTCCGCTCCATTCGTATATGACGACTCCGATGAGAGACCCCAGAGAAACTCCTATCCATGATGGCATGAAGACTCCAATGCTCTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

670

Amino Acids

76.02

Weight (kDa)

5.7

Isoelectric Point (pI)

37.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5_N	PF11942	56 - 152	6.3e-06	Spt5 transcription elongation factor, acidic N-terminal
Spt5-NGN	PF03439	158 - 240	7.9e-24	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	250 - 380	1.6e-45	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	406 - 455	4.7e-19	Transcription elongation factor SPT5, second KOW domain
KOW5_SPT5	PF23290	549 - 590	2.5e-13	Transcription elongation factor SPT5, fifth KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1466, 1677
Acc36I	ACCTGC	1 cut(s)	1156
AccBSI	CCGCTC	2 cut(s)	1879, 1927
AccII	CGCG	4 cut(s)	12, 494, 1689, 1802
AciI	CCGC	8 cut(s)	10, 12, 1262, 1687, 1689, 1800, 1877, 1925
AclWI	GGATC	5 cut(s)	41, 147, 251, 1856, 1904
AcoI	YGGCCR	1 cut(s)	1573
AcsI	RAATTY	5 cut(s)	164, 399, 1136, 1255, 1659
AcuI	CTGAAG	1 cut(s)	1259
AfaI	GTAC	2 cut(s)	978, 1364
AfiI	CCNNNNNNNGG	2 cut(s)	272, 814
AflIII	ACRYGT	1 cut(s)	1486
AgsI	TTSAA	3 cut(s)	179, 356, 1133
AjnI	CCWGG	1 cut(s)	247
AjuI	GAANNNNNNNTTGG	2 cut(s)	792, 824
AloI	GAACNNNNNNTCC	2 cut(s)	1278, 1310
AluBI	AGCT	4 cut(s)	446, 650, 968, 1098
AluI	AGCT	4 cut(s)	446, 650, 968, 1098
Alw26I	GTCTC	4 cut(s)	992, 1007, 1841, 1950
AlwI	GGATC	5 cut(s)	41, 147, 251, 1856, 1904
Ama87I	CYCGRG	2 cut(s)	221, 265
AoxI	GGCC	5 cut(s)	57, 910, 1318, 1573, 1619
ApeKI	GCWGC	3 cut(s)	439, 1415, 1444
ApoI	RAATTY	5 cut(s)	164, 399, 1136, 1255, 1659
AspLEI	GCGC	4 cut(s)	9, 494, 532, 666
AspS9I	GGNCC	4 cut(s)	58, 466, 805, 1619
AsuC2I	CCSGG	1 cut(s)	326
AsuII	TTCGAA	1 cut(s)	1190
AvaI	CYCGRG	2 cut(s)	221, 265
AvaII	GGWCC	2 cut(s)	466, 805
BaeGI	GKGCMC	1 cut(s)	504
BalI	TGGCCA	1 cut(s)	1575
BanII	GRGCYC	1 cut(s)	222
BauI	CACGAG	1 cut(s)	503
BbsI	GAAGAC	4 cut(s)	81, 210, 285, 1997
BbvI	GCAGC	3 cut(s)	451, 1402, 1456
BccI	CCATC	6 cut(s)	266, 407, 475, 568, 706, 1976
BceAI	ACGGC	1 cut(s)	247
BcgI	CGANNNNNNTGC	6 cut(s)	428, 462, 1071, 1105, 1778, 1812
BciT130I	CCWGG	1 cut(s)	249
BclI	TGATCA	2 cut(s)	570, 1438
BcnI	CCSGG	1 cut(s)	326
BcoDI	GTCTC	4 cut(s)	992, 1007, 1841, 1950
BfaI	CTAG	3 cut(s)	908, 1557, 1650
BfmI	CTRYAG	1 cut(s)	1149
BfoI	RGCGCY	1 cut(s)	10
BfuAI	ACCTGC	1 cut(s)	1156
BglII	AGATCT	1 cut(s)	1246
BisI	GCNGC	5 cut(s)	10, 440, 1416, 1445, 1800
BlsI	GCNGC	5 cut(s)	11, 441, 1417, 1446, 1801
BmcAI	AGTACT	2 cut(s)	978, 1364
Bme1390I	CCNGG	2 cut(s)	249, 326
Bme18I	GGWCC	2 cut(s)	466, 805
BmeT110I	CYCGRG	2 cut(s)	221, 265
BmgT120I	GGNCC	4 cut(s)	58, 466, 805, 1619
BmiI	GGNNCC	3 cut(s)	467, 468, 806
BmrFI	CCNGG	2 cut(s)	249, 326
BmsI	GCATC	6 cut(s)	167, 549, 1213, 1531, 1615, 1630
BpiI	GAAGAC	4 cut(s)	81, 210, 285, 1997
BpmI	CTGGAG	1 cut(s)	1623
Bpu14I	TTCGAA	1 cut(s)	1190
BpuEI	CTTGAG	1 cut(s)	1549
BpuMI	CCSGG	1 cut(s)	326
BsaI	GGTCTC	1 cut(s)	1950
BsaJI	CCNNGG	7 cut(s)	10, 183, 222, 808, 866, 1077, 1687
BsaXI	ACNNNNNCTCC	4 cut(s)	1278, 1308, 1834, 1864
Bsc4I	CCNNNNNNNGG	2 cut(s)	272, 814
Bse1I	ACTGG	2 cut(s)	984, 1537
Bse3DI	GCAATG	2 cut(s)	1089, 1834
BseBI	CCWGG	1 cut(s)	249
BseDI	CCNNGG	7 cut(s)	10, 183, 222, 808, 866, 1077, 1687
BseGI	GGATG	5 cut(s)	486, 783, 829, 835, 1645
BseLI	CCNNNNNNNGG	2 cut(s)	272, 814
BseMI	GCAATG	2 cut(s)	1089, 1834
BseMII	CTCAG	2 cut(s)	207, 1836
BseNI	ACTGG	2 cut(s)	984, 1537
BseRI	GAGGAG	7 cut(s)	107, 110, 123, 145, 853, 856, 927
BseSI	GKGCMC	1 cut(s)	504
BseXI	GCAGC	3 cut(s)	451, 1402, 1456
BsgI	GTGCAG	1 cut(s)	927
Bsh1236I	CGCG	4 cut(s)	12, 494, 1689, 1802
Bsh1285I	CGRYCG	4 cut(s)	313, 498, 1171, 1693
BshFI	GGCC	5 cut(s)	59, 912, 1320, 1575, 1621
BsiEI	CGRYCG	4 cut(s)	313, 498, 1171, 1693
BsiHKCI	CYCGRG	2 cut(s)	221, 265
BsiSI	CCGG	1 cut(s)	326
BslFI	GGGAC	6 cut(s)	479, 818, 918, 1660, 1723, 1855
BslI	CCNNNNNNNGG	2 cut(s)	272, 814
BsmAI	GTCTC	4 cut(s)	992, 1007, 1841, 1950
BsmBI	CGTCTC	1 cut(s)	1007
BsmFI	GGGAC	6 cut(s)	479, 818, 918, 1660, 1723, 1855
BsmI	GAATGC	1 cut(s)	1168
BsnI	GGCC	5 cut(s)	59, 912, 1320, 1575, 1621
Bso31I	GGTCTC	1 cut(s)	1950
BsoBI	CYCGRG	2 cut(s)	221, 265
Bsp119I	TTCGAA	1 cut(s)	1190
Bsp1286I	GDGCHC	2 cut(s)	222, 504
BspACI	CCGC	8 cut(s)	10, 12, 1262, 1687, 1689, 1800, 1877, 1925
BspANI	GGCC	5 cut(s)	59, 912, 1320, 1575, 1621
BspCNI	CTCAG	2 cut(s)	208, 1837
BspFNI	CGCG	4 cut(s)	12, 494, 1689, 1802
BspHI	TCATGA	1 cut(s)	1890
BspLI	GGNNCC	3 cut(s)	467, 468, 806
BspMAI	CTGCAG	1 cut(s)	1153
BspMI	ACCTGC	1 cut(s)	1156
BspPI	GGATC	5 cut(s)	41, 147, 251, 1856, 1904
BspT104I	TTCGAA	1 cut(s)	1190
BspTNI	GGTCTC	1 cut(s)	1950
BsrBI	CCGCTC	2 cut(s)	1879, 1927
BsrDI	GCAATG	2 cut(s)	1089, 1834
BsrI	ACTGG	2 cut(s)	984, 1537
BssECI	CCNNGG	7 cut(s)	10, 183, 222, 808, 866, 1077, 1687
BssSI	CACGAG	1 cut(s)	503
BssT1I	CCWWGG	2 cut(s)	808, 866
Bst2BI	CACGAG	1 cut(s)	503
Bst2UI	CCWGG	1 cut(s)	249
Bst4CI	ACNGT	6 cut(s)	311, 853, 948, 1078, 1759, 1815
Bst6I	CTCTTC	8 cut(s)	10, 48, 78, 192, 364, 776, 917, 1609
BstBI	TTCGAA	1 cut(s)	1190
BstC8I	GCNNGC	5 cut(s)	444, 652, 662, 1100, 1420
BstDEI	CTNAG	2 cut(s)	216, 1845
BstDSI	CCRYGG	3 cut(s)	10, 1077, 1687
BstEII	GGTNACC	1 cut(s)	1456
BstF5I	GGATG	5 cut(s)	486, 783, 829, 835, 1645
BstFNI	CGCG	4 cut(s)	12, 494, 1689, 1802
BstH2I	RGCGCY	1 cut(s)	10
BstHHI	GCGC	4 cut(s)	9, 494, 532, 666
BstMAI	GTCTC	4 cut(s)	992, 1007, 1841, 1950
BstMCI	CGRYCG	4 cut(s)	313, 498, 1171, 1693
BstMWI	GCNNNNNNNGC	1 cut(s)	1450
BstNI	CCWGG	1 cut(s)	249
BstNSI	RCATGY	2 cut(s)	664, 1490
BstPI	GGTNACC	1 cut(s)	1456
BstSCI	CCNGG	2 cut(s)	247, 324
BstSFI	CTRYAG	1 cut(s)	1149
BstSLI	GKGCMC	1 cut(s)	504
BstUI	CGCG	4 cut(s)	12, 494, 1689, 1802
BstV1I	GCAGC	3 cut(s)	451, 1402, 1456
BstV2I	GAAGAC	4 cut(s)	81, 210, 285, 1997
BstX2I	RGATCY	4 cut(s)	46, 1246, 1861, 1909
BstYI	RGATCY	4 cut(s)	46, 1246, 1861, 1909
BsuRI	GGCC	5 cut(s)	59, 912, 1320, 1575, 1621
BtgI	CCRYGG	3 cut(s)	10, 1077, 1687
BtgZI	GCGATG	1 cut(s)	1191
BtsCI	GGATG	5 cut(s)	486, 783, 829, 835, 1645
BtsIMutI	CAGTG	1 cut(s)	1811
BveI	ACCTGC	1 cut(s)	1156
Cac8I	GCNNGC	5 cut(s)	444, 652, 662, 1100, 1420
CciI	TCATGA	1 cut(s)	1890
CfoI	GCGC	4 cut(s)	9, 494, 532, 666
Cfr13I	GGNCC	4 cut(s)	58, 466, 805, 1619
Cfr42I	CCGCGG	2 cut(s)	13, 1690
Csp6I	GTAC	2 cut(s)	977, 1363
CviQI	GTAC	2 cut(s)	977, 1363
DdeI	CTNAG	2 cut(s)	216, 1845
DraI	TTTAAA	2 cut(s)	1054, 1369
EaeI	YGGCCR	1 cut(s)	1573
Eam1104I	CTCTTC	8 cut(s)	10, 48, 78, 192, 364, 776, 917, 1609
EarI	CTCTTC	8 cut(s)	10, 48, 78, 192, 364, 776, 917, 1609
Eco130I	CCWWGG	2 cut(s)	808, 866
Eco147I	AGGCCT	2 cut(s)	912, 1320
Eco24I	GRGCYC	1 cut(s)	222
Eco31I	GGTCTC	1 cut(s)	1950
Eco47I	GGWCC	2 cut(s)	466, 805
Eco57I	CTGAAG	1 cut(s)	1259
Eco88I	CYCGRG	2 cut(s)	221, 265
Eco91I	GGTNACC	1 cut(s)	1456
EcoO109I	RGGNCCY	4 cut(s)	58, 466, 805, 1619
EcoO65I	GGTNACC	1 cut(s)	1456
EcoRII	CCWGG	1 cut(s)	247
EcoT14I	CCWWGG	2 cut(s)	808, 866
EcoT38I	GRGCYC	1 cut(s)	222
ErhI	CCWWGG	2 cut(s)	808, 866
Esp3I	CGTCTC	1 cut(s)	1007
FalI	AAGNNNNNCTT	4 cut(s)	45, 77, 792, 824
FaqI	GGGAC	6 cut(s)	479, 818, 918, 1660, 1723, 1855
FauI	CCCGC	1 cut(s)	1255
FauNDI	CATATG	2 cut(s)	567, 1299
FbaI	TGATCA	2 cut(s)	570, 1438
Fnu4HI	GCNGC	5 cut(s)	10, 440, 1416, 1445, 1800
FokI	GGATG	5 cut(s)	493, 790, 836, 842, 1652
FriOI	GRGCYC	1 cut(s)	222
Fsp4HI	GCNGC	5 cut(s)	10, 440, 1416, 1445, 1800
FspBI	CTAG	3 cut(s)	908, 1557, 1650
GlaI	GCGC	4 cut(s)	8, 493, 531, 665
GluI	GCNGC	5 cut(s)	10, 440, 1416, 1445, 1800
GsuI	CTGGAG	1 cut(s)	1623
HaeII	RGCGCY	1 cut(s)	10
HaeIII	GGCC	5 cut(s)	59, 912, 1320, 1575, 1621
HapII	CCGG	1 cut(s)	326
HhaI	GCGC	4 cut(s)	9, 494, 532, 666
Hin6I	GCGC	4 cut(s)	7, 492, 530, 664
HinP1I	GCGC	4 cut(s)	7, 492, 530, 664
HindIII	AAGCTT	1 cut(s)	648
HpaII	CCGG	1 cut(s)	326
Hpy188III	TCNNGA	8 cut(s)	50, 590, 881, 1010, 1325, 1566, 1602, 1891
Hpy99I	CGWCG	5 cut(s)	74, 233, 257, 293, 1770
HpyAV	CCTTC	9 cut(s)	82, 291, 794, 1210, 1234, 1270, 1331, 1381, 1426
HpyCH4III	ACNGT	6 cut(s)	311, 853, 948, 1078, 1759, 1815
HpyCH4IV	ACGT	1 cut(s)	291
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1450
HpyF3I	CTNAG	2 cut(s)	216, 1845
HpySE526I	ACGT	1 cut(s)	291
HspAI	GCGC	4 cut(s)	7, 492, 530, 664
KflI	GGGWCCC	1 cut(s)	466
Ksp22I	TGATCA	2 cut(s)	570, 1438
KspI	CCGCGG	2 cut(s)	13, 1690
LmnI	GCTCC	3 cut(s)	97, 1884, 1932
Lsp1109I	GCAGC	3 cut(s)	451, 1402, 1456
LweI	GCATC	6 cut(s)	167, 549, 1213, 1531, 1615, 1630
MaeI	CTAG	3 cut(s)	908, 1557, 1650
MaeII	ACGT	1 cut(s)	291
MaeIII	GTNAC	5 cut(s)	847, 1040, 1390, 1456, 1753
MbiI	CCGCTC	2 cut(s)	1879, 1927
MfeI	CAATTG	1 cut(s)	582
MflI	RGATCY	4 cut(s)	46, 1246, 1861, 1909
MhlI	GDGCHC	2 cut(s)	222, 504
MlsI	TGGCCA	1 cut(s)	1575
MluNI	TGGCCA	1 cut(s)	1575
MlyI	GAGTC	5 cut(s)	347, 1745, 1843, 1939, 1987
MmeI	TCCRAC	1 cut(s)	321
Mox20I	TGGCCA	1 cut(s)	1575
MscI	TGGCCA	1 cut(s)	1575
MseI	TTAA	3 cut(s)	705, 1053, 1368
MslI	CAYNNNNRTG	1 cut(s)	1427
Msp20I	TGGCCA	1 cut(s)	1575
MspA1I	CMGCKG	3 cut(s)	12, 1098, 1689
MspI	CCGG	1 cut(s)	326
MspR9I	CCNGG	2 cut(s)	249, 326
MssI	GTTTAAAC	1 cut(s)	1054
MunI	CAATTG	1 cut(s)	582
Mva1269I	GAATGC	1 cut(s)	1168
MvaI	CCWGG	1 cut(s)	249
MvnI	CGCG	4 cut(s)	12, 494, 1689, 1802
MwoI	GCNNNNNNNGC	1 cut(s)	1450
NciI	CCSGG	1 cut(s)	326
NdeI	CATATG	2 cut(s)	567, 1299
NlaIV	GGNNCC	3 cut(s)	467, 468, 806
NmeAIII	GCCGAG	1 cut(s)	208
NmuCI	GTSAC	3 cut(s)	1040, 1456, 1753
NspI	RCATGY	2 cut(s)	664, 1490
NspV	TTCGAA	1 cut(s)	1190
PaeI	GCATGC	1 cut(s)	664
PagI	TCATGA	1 cut(s)	1890
PceI	AGGCCT	2 cut(s)	912, 1320
PciI	ACATGT	1 cut(s)	1486
PcsI	WCGNNNNNNNCGW	2 cut(s)	288, 1940
PctI	GAATGC	1 cut(s)	1168
PfeI	GAWTC	6 cut(s)	212, 389, 862, 985, 1378, 1784
PfoI	TCCNGGA	1 cut(s)	324
PkrI	GCNGC	5 cut(s)	11, 441, 1417, 1446, 1801
Ple19I	CGATCG	2 cut(s)	498, 1171
PleI	GAGTC	5 cut(s)	346, 1744, 1843, 1939, 1987
PmeI	GTTTAAAC	1 cut(s)	1054
PpsI	GAGTC	5 cut(s)	346, 1744, 1843, 1939, 1987
PpuMI	RGGWCCY	2 cut(s)	466, 805
PscI	ACATGT	1 cut(s)	1486
PsiI	TTATAA	2 cut(s)	1466, 1677
Psp5II	RGGWCCY	2 cut(s)	466, 805
Psp6I	CCWGG	1 cut(s)	247
PspEI	GGTNACC	1 cut(s)	1456
PspGI	CCWGG	1 cut(s)	247
PspN4I	GGNNCC	3 cut(s)	467, 468, 806
PspPI	GGNCC	4 cut(s)	58, 466, 805, 1619
PspPPI	RGGWCCY	2 cut(s)	466, 805
PstI	CTGCAG	1 cut(s)	1153
PsuI	RGATCY	4 cut(s)	46, 1246, 1861, 1909
PvuI	CGATCG	2 cut(s)	498, 1171
PvuII	CAGCTG	1 cut(s)	1098
RsaI	GTAC	2 cut(s)	978, 1364
RsaNI	GTAC	2 cut(s)	977, 1363
RseI	CAYNNNNRTG	1 cut(s)	1427
SacII	CCGCGG	2 cut(s)	13, 1690
SaqAI	TTAA	3 cut(s)	705, 1053, 1368
SatI	GCNGC	5 cut(s)	10, 440, 1416, 1445, 1800
Sau96I	GGNCC	4 cut(s)	58, 466, 805, 1619
SbfI	CCTGCAGG	1 cut(s)	1153
ScaI	AGTACT	2 cut(s)	978, 1364
SchI	GAGTC	5 cut(s)	347, 1745, 1843, 1939, 1987
ScrFI	CCNGG	2 cut(s)	249, 326
SdaI	CCTGCAGG	1 cut(s)	1153
SduI	GDGCHC	2 cut(s)	222, 504
SfaNI	GCATC	6 cut(s)	167, 549, 1213, 1531, 1615, 1630
SfcI	CTRYAG	1 cut(s)	1149
Sfr303I	CCGCGG	2 cut(s)	13, 1690
SfuI	TTCGAA	1 cut(s)	1190
SgrBI	CCGCGG	2 cut(s)	13, 1690
SinI	GGWCC	2 cut(s)	466, 805
SmiMI	CAYNNNNRTG	1 cut(s)	1427
SmlI	CTYRAG	1 cut(s)	1564
SmoI	CTYRAG	1 cut(s)	1564
SphI	GCATGC	1 cut(s)	664
Sse8387I	CCTGCAGG	1 cut(s)	1153
SseBI	AGGCCT	2 cut(s)	912, 1320
SsiI	CCGC	8 cut(s)	10, 12, 1262, 1687, 1689, 1800, 1877, 1925
SspMI	CTAG	3 cut(s)	908, 1557, 1650
StuI	AGGCCT	2 cut(s)	912, 1320
StyD4I	CCNGG	2 cut(s)	247, 324
StyI	CCWWGG	2 cut(s)	808, 866
TaaI	ACNGT	6 cut(s)	311, 853, 948, 1078, 1759, 1815
TaiI	ACGT	1 cut(s)	294
TaqI	TCGA	7 cut(s)	161, 189, 1091, 1122, 1190, 1609, 1768
TatI	WGTACW	2 cut(s)	976, 1362
TauI	GCSGC	2 cut(s)	12, 1802
TfiI	GAWTC	6 cut(s)	212, 389, 862, 985, 1378, 1784
Tru1I	TTAA	3 cut(s)	705, 1053, 1368
Tru9I	TTAA	3 cut(s)	705, 1053, 1368
TscAI	CASTG	1 cut(s)	1818
TseFI	GTSAC	3 cut(s)	1040, 1456, 1753
TseI	GCWGC	3 cut(s)	439, 1415, 1444
Tsp45I	GTSAC	3 cut(s)	1040, 1456, 1753
TspGWI	ACGGA	2 cut(s)	93, 736
TspRI	CASTG	1 cut(s)	1818
VpaK11BI	GGWCC	2 cut(s)	466, 805
XapI	RAATTY	5 cut(s)	164, 399, 1136, 1255, 1659
XceI	RCATGY	2 cut(s)	664, 1490
XspI	CTAG	3 cut(s)	908, 1557, 1650
ZrmI	AGTACT	2 cut(s)	978, 1364

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.