Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000030.1

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Forward (+)

118242 .. 126619

Gene Structure 8378 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0000030.1_g000027.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3264 bp

atgccccgtcacagagacgaagacgacgacgagatggagccggacgaggacgagtacgacccggaccaaggtctagacgacgtcgacgtcgacgacgaagaggaggaggaggaggaggaggaggaccgaggagggcggaggtccagtcagaagcggaggagatcgagcttcattgatgacgtggccgaggaggacgacgaggaggaagaggaggactacgaggacgaggattacggcgcctaccgtggcggtggcggtgcgaggaggaggaggaggaggaccgaggagggcggaggtccagaggacgacgaggaggaagaggaggactacgaggacgaggattacggcgcctaccgtggcggtggcggtggcggtgggagtcgtaagaagagcaaaaagaagccctccggtgcggattttcttgatctcgaagcggtggtggattcggatgaggaggaagaggaggatgagggcgaggacgactttattgattccggagctgatatacctgaggagggcgatggtagaaggatgcatcatcgcccattgatggatgaccaagagcaagaagatgtcgaggctctggaaagaagaattcaggcaagatatgcaaagcaacatcatacagaagatactgaggagaccactgatgtggatcagcaagctcttttaccttctgttttggacccaaagctgtggatggtgaaatgtgcgattggtaaagaacgagaggtagctgcttgcttaatgcaaaagtatattgataaacctgaattgaacatcaggtctgttattgctcttgaccacctgaagaactatatatacgttgaagcggaaaaagaagcccatgtgagggaggcttgcaaaggattgcgcaatatatttacccagaaaatagcgcttgttcctattagagaaatgactgatgttctttccgttgaaagcaaggcaattgatatttctagggatacttgggtcagaatgaagattgggatatataaaggggaccttgccaaggttgttgatgtggataatgtacgacagagagttacagtaaaattaattccaagaattgatttacaagctcttgcaaataaactggagggcagagaagttgtgaaaaagaaagcgtttgtacctccgccacgttttatgaatattgatgaagctagagaactgcatattcgtgtggagcgtaaacgagatccaatgacgggtgactactttgaaaacattgagaaaatgatgtttaaagatggtttcttgtataaaatagtatcaatgaaatcaattagtactcaaaacatacatcccacttttgatgaactggaaaagtttcggaaacctggggagaatggggaaggagatgttgccagtctgtcaactttattttcaaacagaaagaaaggacactttgtgaagggtgatgcagtcattgtcatcaagggagacctaaaaaacttgaaagggcgggttgagaaagttgaggaagggactgtgcatatcagacccgacatgaaagacctcccaaaaactcttgccataaatgaaaaagaactttgcaagtactttgaacctgggaatcatgtgaaggttgtatctggtactcaagaaggtgcaactggtatggttgtcaaggtcgatcagcatgtgctgatcattttatctgatacaactaaggaacatctccgtgtgtttgctgatgatgttgtggagagttctgaggtgacatctggtattaccaaaattggggactatgagcttcatgatcttgtgttactggataataatagctttggtgtaattatacgtgttgaaactgaagcatgtcaggtccttaagggagttcctgagagacctgaggttgcccttgtcaaattaagagagatcaaatgcaagattgagaaaaagcttagtgtgcaagattgttttaagaacacaatatctgtgaaagatgttgtgagggtcgttgaaggtccttccaagggtaaacaaggtcccgtggaacacatatacagaggagtcttattcatttatgatcgccatcacatagagcatgcaggctttatctgtgttaagtcacattcttgtcgtgttgtaggaggatcacgtgccaatggtgatagaaatggtgactcctactctagatttgatcatcttcgagctcctcctcttattccccagtcccccaggaggtttcctagaggaggccctcctaataactttgggggaagaaatagaggtggaagagggcatgatggattggttgggactacagtaaagattcgccagggtgcctataagggttaccgtgggcgcgttgttgaggttaagggaactagtgtacgggtggaacttgagtcccaaatgaaagttgtgacagttgaccggaattgtatttctgataacgtggctattacaacaccgtatcgtgatacatctagctatggtatgggaagtcagaccccaattcatccttctcgaactcctctccatccatatatgacgccaatgagggatgctggagcaacgcctattcatgatggcatgaggactccaatgcgtgaccgagcatggaatccatatgcacctatgagtccagcaagggatagctgggaggatggaaaccctggttcttggggttctagcccacagtatcagccaggaagtcctccctcacggacgtacgaagctccaactcctggttcaggttgggccaacactcctggtgggaattatagtgaagctggaacaccaagggacagtactggttatgcaaatgctcctagcccatacttgccgtcaactcccggtgggcagcctatgacaccaaactcagcatcctatcttcccggaactcctggagggcagccaatgacaccagggactggtggtctggatatgatgtctcctgttataggaggggacaatgaaggaccatggttcatgccagatatactggtcaatgtacgcaattctggagaggaaactactggaattgtcagagaagtgctcctggatggctcatgtagggtagctcttggctcgggtggcaatggtgatactgtaactgtacttccaaatgaaatcgaggtagtcgtaccaaggaagaatgataagatcaaaatcatgggtggctcattacgcggtgccactggcaagctgattggtgtcgatggcacggatggaattgtcaaggttgatgacactcttgatgttaaaatattagacttggctattttgtcaaaactaggtcaatcataa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

1087

Amino Acids

120.72

Weight (kDa)

5.08

Isoelectric Point (pI)

47.48

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	86
AatII	GACGTC	2 cut(s)	84, 90
Acc16I	TGCGCA	1 cut(s)	875
AccB1I	GGYRCC	4 cut(s)	236, 347, 2294, 3149
AccB7I	CCANNNNNTGG	2 cut(s)	2702, 2888
AccI	GTMKAC	2 cut(s)	84, 90
AccII	CGCG	2 cut(s)	2319, 3147
AccIII	TCCGGA	1 cut(s)	494
AclWI	GGATC	3 cut(s)	663, 1199, 2113
AcoI	YGGCCR	1 cut(s)	183
AcsI	RAATTY	1 cut(s)	594
AcuI	CTGAAG	2 cut(s)	830, 1842
AcvI	CACGTG	1 cut(s)	2111
AcyI	GRCGYC	5 cut(s)	81, 87, 237, 348, 2507
AdeI	CACNNNGTG	1 cut(s)	1417
AfeI	AGCGCT	1 cut(s)	900
AflII	CTTAAG	1 cut(s)	1838
AflIII	ACRYGT	1 cut(s)	1810
AgsI	TTSAA	9 cut(s)	778, 830, 941, 1229, 1395, 1465, 1574, 1817, 1973
AhdI	GACNNNNNGTC	1 cut(s)	2892
AhlI	ACTAGT	1 cut(s)	2339
AjiI	CACGTC	1 cut(s)	181
AjuI	GAANNNNNNNTTGG	4 cut(s)	2461, 2493, 2689, 2721
AleI	CACNNNNGTG	1 cut(s)	650
Alw21I	GWGCWC	2 cut(s)	2167, 3015
Alw26I	GTCTC	5 cut(s)	9, 635, 1443, 1849, 2913
AlwI	GGATC	3 cut(s)	663, 1199, 2113
AlwNI	CAGNNNCTG	1 cut(s)	2888
Ama87I	CYCGRG	1 cut(s)	3046
Aor13HI	TCCGGA	1 cut(s)	494
Aor51HI	AGCGCT	1 cut(s)	900
AoxI	GGCC	3 cut(s)	183, 2209, 2715
ApeKI	GCWGC	3 cut(s)	737, 2818, 2869
ApoI	RAATTY	1 cut(s)	594
AseI	ATTAAT	1 cut(s)	1061
Asp700I	GAANNNNTTC	1 cut(s)	1335
AspLEI	GCGC	5 cut(s)	239, 350, 876, 901, 2319
AsuC2I	CCSGG	3 cut(s)	62, 2811, 2853
AsuHPI	GGTGA	7 cut(s)	715, 1229, 1436, 1738, 2132, 2144, 3071
AvaI	CYCGRG	1 cut(s)	3046
AxyI	CCTNAGG	2 cut(s)	510, 1860
BaeI	ACNNNNGTAYC	2 cut(s)	960, 993
BanI	GGYRCC	4 cut(s)	236, 347, 2294, 3149
BanII	GRGCYC	1 cut(s)	2167
BarI	GAAGNNNNNNTAC	2 cut(s)	3060, 3092
BbrPI	CACGTG	1 cut(s)	2111
BbsI	GAAGAC	1 cut(s)	27
Bbv12I	GWGCWC	2 cut(s)	2167, 3015
BbvI	GCAGC	3 cut(s)	724, 2830, 2881
BceAI	ACGGC	3 cut(s)	250, 361, 2785
BciVI	GTATCC	1 cut(s)	961
BclI	TGATCA	2 cut(s)	1656, 2152
BcnI	CCSGG	3 cut(s)	62, 2811, 2853
BcoDI	GTCTC	5 cut(s)	9, 635, 1443, 1849, 2913
BcuI	ACTAGT	1 cut(s)	2339
BfmI	CTRYAG	1 cut(s)	2274
BfoI	RGCGCY	3 cut(s)	240, 351, 902
BfrI	CTTAAG	1 cut(s)	1838
BfuI	GTATCC	1 cut(s)	961
BisI	GCNGC	3 cut(s)	738, 2819, 2870
BlsI	GCNGC	3 cut(s)	739, 2820, 2871
BmcAI	AGTACT	3 cut(s)	1297, 1568, 2767
BmeRI	GACNNNNNGTC	1 cut(s)	2892
BmeT110I	CYCGRG	1 cut(s)	3046
BmgBI	CACGTC	1 cut(s)	181
BmiI	GGNNCC	8 cut(s)	39, 238, 349, 687, 1007, 1999, 2296, 3151
BmrI	ACTGGG	1 cut(s)	2176
BmsI	GCATC	5 cut(s)	522, 544, 1417, 2509, 2849
BmuI	ACTGGG	1 cut(s)	2176
BoxI	GACNNNNGTC	1 cut(s)	69
BpiI	GAAGAC	1 cut(s)	27
BplI	GAGNNNNNCTC	2 cut(s)	2997, 3029
BpmI	CTGGAG	4 cut(s)	1121, 2544, 2883, 3000
BpuEI	CTTGAG	2 cut(s)	1593, 2378
BpuMI	CCSGG	3 cut(s)	62, 2811, 2853
BsaAI	YACGTR	2 cut(s)	1811, 2111
BsaBI	GATNNNNATC	3 cut(s)	654, 2043, 3125
BsaHI	GRCGYC	5 cut(s)	81, 87, 237, 348, 2507
BsaI	GGTCTC	3 cut(s)	635, 1443, 1849
BsaWI	WCCGGW	3 cut(s)	407, 494, 2388
BsaXI	ACNNNNNCTCC	2 cut(s)	130, 160
Bse21I	CCTNAGG	2 cut(s)	510, 1860
Bse3DI	GCAATG	1 cut(s)	3061
Bse8I	GATNNNNATC	3 cut(s)	654, 2043, 3125
BseAI	TCCGGA	1 cut(s)	494
BseJI	GATNNNNATC	3 cut(s)	654, 2043, 3125
BseMI	GCAATG	1 cut(s)	3061
BseMII	CTCAG	6 cut(s)	501, 627, 1713, 1842, 1851, 2850
BseXI	GCAGC	3 cut(s)	724, 2830, 2881
BseYI	CCCAGC	1 cut(s)	2613
Bsh1236I	CGCG	2 cut(s)	2319, 3147
BshFI	GGCC	3 cut(s)	185, 2211, 2717
BshNI	GGYRCC	4 cut(s)	236, 347, 2294, 3149
BsiHKAI	GWGCWC	2 cut(s)	2167, 3015
BsiHKCI	CYCGRG	1 cut(s)	3046
BsiSI	CCGG	7 cut(s)	41, 62, 408, 495, 2389, 2811, 2853
BsiWI	CGTACG	1 cut(s)	2685
BsmAI	GTCTC	5 cut(s)	9, 635, 1443, 1849, 2913
BsmBI	CGTCTC	1 cut(s)	9
BsnI	GGCC	3 cut(s)	185, 2211, 2717
Bso31I	GGTCTC	3 cut(s)	635, 1443, 1849
BsoBI	CYCGRG	1 cut(s)	3046
Bsp1286I	GDGCHC	2 cut(s)	2167, 3015
Bsp13I	TCCGGA	1 cut(s)	494
Bsp19I	CCATGG	1 cut(s)	2939
BspANI	GGCC	3 cut(s)	185, 2211, 2717
BspCNI	CTCAG	6 cut(s)	502, 628, 1714, 1843, 1852, 2849
BspEI	TCCGGA	1 cut(s)	494
BspFNI	CGCG	2 cut(s)	2319, 3147
BspHI	TCATGA	2 cut(s)	1765, 2539
BspLI	GGNNCC	8 cut(s)	39, 238, 349, 687, 1007, 1999, 2296, 3151
BspPI	GGATC	3 cut(s)	663, 1199, 2113
BspQI	GCTCTTC	1 cut(s)	383
BspT107I	GGYRCC	4 cut(s)	236, 347, 2294, 3149
BspTI	CTTAAG	1 cut(s)	1838
BspTNI	GGTCTC	3 cut(s)	635, 1443, 1849
BsrDI	GCAATG	1 cut(s)	3061
BssNI	GRCGYC	5 cut(s)	81, 87, 237, 348, 2507
BssT1I	CCWWGG	6 cut(s)	67, 1014, 1983, 2756, 2939, 3104
Bst6I	CTCTTC	6 cut(s)	93, 201, 312, 383, 453, 2242
BstACI	GRCGYC	5 cut(s)	81, 87, 237, 348, 2507
BstAFI	CTTAAG	1 cut(s)	1838
BstAPI	GCANNNNNTGC	1 cut(s)	608
BstBAI	YACGTR	2 cut(s)	1811, 2111
BstC8I	GCNNGC	6 cut(s)	663, 742, 862, 2058, 2062, 3161
BstDEI	CTNAG	8 cut(s)	510, 636, 1677, 1722, 1851, 1860, 1913, 2836
BstDSI	CCRYGG	5 cut(s)	244, 355, 2001, 2311, 2939
BstEII	GGTNACC	1 cut(s)	2306
BstENI	CCTNNNNNAGG	4 cut(s)	1013, 2205, 2706, 2916
BstFNI	CGCG	2 cut(s)	2319, 3147
BstH2I	RGCGCY	3 cut(s)	240, 351, 902
BstHHI	GCGC	5 cut(s)	239, 350, 876, 901, 2319
BstMAI	GTCTC	5 cut(s)	9, 635, 1443, 1849, 2913
BstMWI	GCNNNNNNNGC	4 cut(s)	608, 1918, 3051, 3144
BstNSI	RCATGY	3 cut(s)	1652, 1830, 2060
BstPAI	GACNNNNGTC	1 cut(s)	69
BstPI	GGTNACC	1 cut(s)	2306
BstSFI	CTRYAG	1 cut(s)	2274
BstUI	CGCG	2 cut(s)	2319, 3147
BstV1I	GCAGC	3 cut(s)	724, 2830, 2881
BstV2I	GAAGAC	1 cut(s)	27
BstX2I	RGATCY	1 cut(s)	1204
BstXI	CCANNNNNNTGG	2 cut(s)	652, 696
BstYI	RGATCY	1 cut(s)	1204
Bsu36I	CCTNAGG	2 cut(s)	510, 1860
BsuI	GTATCC	1 cut(s)	961
BsuRI	GGCC	3 cut(s)	185, 2211, 2717
BtgI	CCRYGG	5 cut(s)	244, 355, 2001, 2311, 2939
BtgZI	GCGATG	2 cut(s)	524, 534
BtrI	CACGTC	1 cut(s)	181
BtsIMutI	CAGTG	2 cut(s)	645, 3153
Cac8I	GCNNGC	6 cut(s)	663, 742, 862, 2058, 2062, 3161
CaiI	CAGNNNCTG	1 cut(s)	2888
CciI	TCATGA	2 cut(s)	1765, 2539
CfoI	GCGC	5 cut(s)	239, 350, 876, 901, 2319
CseI	GACGC	1 cut(s)	2515
DdeI	CTNAG	8 cut(s)	510, 636, 1677, 1722, 1851, 1860, 1913, 2836
DinI	GGCGCC	2 cut(s)	238, 349
DraI	TTTAAA	1 cut(s)	1252
DraIII	CACNNNGTG	1 cut(s)	1417
DrdI	GACNNNNNNGTC	1 cut(s)	86
DriI	GACNNNNNGTC	1 cut(s)	2892
DseDI	GACNNNNNNGTC	1 cut(s)	86
EaeI	YGGCCR	1 cut(s)	183
Eam1104I	CTCTTC	6 cut(s)	93, 201, 312, 383, 453, 2242
Eam1105I	GACNNNNNGTC	1 cut(s)	2892
EarI	CTCTTC	6 cut(s)	93, 201, 312, 383, 453, 2242
EciI	GGCGGA	3 cut(s)	151, 306, 1131
Ecl136II	GAGCTC	1 cut(s)	2165
Eco130I	CCWWGG	6 cut(s)	67, 1014, 1983, 2756, 2939, 3104
Eco24I	GRGCYC	1 cut(s)	2167
Eco31I	GGTCTC	3 cut(s)	635, 1443, 1849
Eco47III	AGCGCT	1 cut(s)	900
Eco53kI	GAGCTC	1 cut(s)	2165
Eco57I	CTGAAG	2 cut(s)	830, 1842
Eco72I	CACGTG	1 cut(s)	2111
Eco81I	CCTNAGG	2 cut(s)	510, 1860
Eco88I	CYCGRG	1 cut(s)	3046
Eco91I	GGTNACC	1 cut(s)	2306
EcoICRI	GAGCTC	1 cut(s)	2165
EcoNI	CCTNNNNNAGG	4 cut(s)	1013, 2205, 2706, 2916
EcoO109I	RGGNCCY	5 cut(s)	1006, 1834, 1976, 1997, 2210
EcoO65I	GGTNACC	1 cut(s)	2306
EcoRI	GAATTC	1 cut(s)	594
EcoT14I	CCWWGG	6 cut(s)	67, 1014, 1983, 2756, 2939, 3104
EcoT22I	ATGCAT	1 cut(s)	537
EcoT38I	GRGCYC	1 cut(s)	2167
EgeI	GGCGCC	2 cut(s)	238, 349
EheI	GGCGCC	2 cut(s)	238, 349
ErhI	CCWWGG	6 cut(s)	67, 1014, 1983, 2756, 2939, 3104
Esp3I	CGTCTC	1 cut(s)	9
FalI	AAGNNNNNCTT	2 cut(s)	993, 1025
FauI	CCCGC	1 cut(s)	1464
FauNDI	CATATG	1 cut(s)	2584
FbaI	TGATCA	2 cut(s)	1656, 2152
FblI	GTMKAC	2 cut(s)	84, 90
Fnu4HI	GCNGC	3 cut(s)	738, 2819, 2870
FriOI	GRGCYC	1 cut(s)	2167
Fsp4HI	GCNGC	3 cut(s)	738, 2819, 2870
FspI	TGCGCA	1 cut(s)	875
GlaI	GCGC	5 cut(s)	238, 349, 875, 900, 2318
GluI	GCNGC	3 cut(s)	738, 2819, 2870
GsaI	CCCAGC	1 cut(s)	2617
GsuI	CTGGAG	4 cut(s)	1121, 2544, 2883, 3000
HaeII	RGCGCY	3 cut(s)	240, 351, 902
HaeIII	GGCC	3 cut(s)	185, 2211, 2717
HapII	CCGG	7 cut(s)	41, 62, 408, 495, 2389, 2811, 2853
HgaI	GACGC	1 cut(s)	2515
HhaI	GCGC	5 cut(s)	239, 350, 876, 901, 2319
Hin1I	GRCGYC	5 cut(s)	81, 87, 237, 348, 2507
Hin6I	GCGC	5 cut(s)	237, 348, 874, 899, 2317
HinP1I	GCGC	5 cut(s)	237, 348, 874, 899, 2317
HincII	GTYRAC	5 cut(s)	85, 91, 1383, 2386, 2805
HindII	GTYRAC	5 cut(s)	85, 91, 1383, 2386, 2805
HindIII	AAGCTT	1 cut(s)	1910
HpaII	CCGG	7 cut(s)	41, 62, 408, 495, 2389, 2811, 2853
HphI	GGTGA	7 cut(s)	715, 1229, 1436, 1738, 2132, 2144, 3071
Hpy166II	GTNNAC	8 cut(s)	85, 91, 1199, 1383, 1991, 2345, 2386, 2805
Hpy8I	GTNNAC	8 cut(s)	85, 91, 1199, 1383, 1991, 2345, 2386, 2805
HpyCH4IV	ACGT	9 cut(s)	81, 87, 180, 825, 1147, 1810, 2110, 2409, 2684
HpyF10VI	GCNNNNNNNGC	4 cut(s)	608, 1918, 3051, 3144
HpyF3I	CTNAG	8 cut(s)	510, 636, 1677, 1722, 1851, 1860, 1913, 2836
HpySE526I	ACGT	9 cut(s)	81, 87, 180, 825, 1147, 1810, 2110, 2409, 2684
Hsp92I	GRCGYC	5 cut(s)	81, 87, 237, 348, 2507
HspAI	GCGC	5 cut(s)	237, 348, 874, 899, 2317
KasI	GGCGCC	2 cut(s)	236, 347
Kpn2I	TCCGGA	1 cut(s)	494
Ksp22I	TGATCA	2 cut(s)	1656, 2152
LguI	GCTCTTC	1 cut(s)	383
LmnI	GCTCC	8 cut(s)	37, 497, 1192, 2170, 2525, 2698, 2788, 3018
Lsp1109I	GCAGC	3 cut(s)	724, 2830, 2881
LweI	GCATC	5 cut(s)	522, 544, 1417, 2509, 2849
MaeII	ACGT	9 cut(s)	81, 87, 180, 825, 1147, 1810, 2110, 2409, 2684
MfeI	CAATTG	1 cut(s)	951
MflI	RGATCY	1 cut(s)	1204
MhlI	GDGCHC	2 cut(s)	2167, 3015
Mly113I	GGCGCC	2 cut(s)	237, 348
MlyI	GAGTC	6 cut(s)	388, 2031, 2129, 2369, 2548, 2605
MmeI	TCCRAC	1 cut(s)	2720
Mph1103I	ATGCAT	1 cut(s)	537
MroI	TCCGGA	1 cut(s)	494
MroXI	GAANNNNTTC	1 cut(s)	1335
MseI	TTAA	9 cut(s)	746, 1061, 1251, 1839, 1880, 1932, 2076, 2331, 3219
MslI	CAYNNNNRTG	3 cut(s)	650, 1185, 1689
MspCI	CTTAAG	1 cut(s)	1838
MspI	CCGG	7 cut(s)	41, 62, 408, 495, 2389, 2811, 2853
MunI	CAATTG	1 cut(s)	951
MvnI	CGCG	2 cut(s)	2319, 3147
MwoI	GCNNNNNNNGC	4 cut(s)	608, 1918, 3051, 3144
NarI	GGCGCC	2 cut(s)	237, 348
NciI	CCSGG	3 cut(s)	62, 2811, 2853
NcoI	CCATGG	1 cut(s)	2939
NdeI	CATATG	1 cut(s)	2584
NlaIV	GGNNCC	8 cut(s)	39, 238, 349, 687, 1007, 1999, 2296, 3151
NmeAIII	GCCGAG	1 cut(s)	211
NmuCI	GTSAC	7 cut(s)	8, 1217, 1726, 2079, 2132, 2377, 2564
NsbI	TGCGCA	1 cut(s)	875
NsiI	ATGCAT	1 cut(s)	537
NspI	RCATGY	3 cut(s)	1652, 1830, 2060
OliI	CACNNNNGTG	1 cut(s)	650
PaeI	GCATGC	1 cut(s)	2060
PagI	TCATGA	2 cut(s)	1765, 2539
PciSI	GCTCTTC	1 cut(s)	383
PcsI	WCGNNNNNNNCGW	7 cut(s)	24, 84, 87, 90, 93, 1192, 3096
PdmI	GAANNNNTTC	1 cut(s)	1335
PfeI	GAWTC	5 cut(s)	443, 491, 1582, 2284, 2578
Pfl23II	CGTACG	1 cut(s)	2685
PflFI	GACNNNGTC	1 cut(s)	80
PflMI	CCANNNNNTGG	2 cut(s)	2702, 2888
PfoI	TCCNGGA	3 cut(s)	2851, 2860, 3015
PkrI	GCNGC	3 cut(s)	739, 2820, 2871
PleI	GAGTC	6 cut(s)	387, 2030, 2129, 2368, 2548, 2604
PluTI	GGCGCC	2 cut(s)	240, 351
PmaCI	CACGTG	1 cut(s)	2111
PmlI	CACGTG	1 cut(s)	2111
PpsI	GAGTC	6 cut(s)	387, 2030, 2129, 2368, 2548, 2604
Ppu21I	YACGTR	2 cut(s)	1811, 2111
PpuMI	RGGWCCY	4 cut(s)	1006, 1834, 1976, 1997
PshAI	GACNNNNGTC	1 cut(s)	69
PshBI	ATTAAT	1 cut(s)	1061
Psp124BI	GAGCTC	1 cut(s)	2167
Psp5II	RGGWCCY	4 cut(s)	1006, 1834, 1976, 1997
PspCI	CACGTG	1 cut(s)	2111
PspEI	GGTNACC	1 cut(s)	2306
PspFI	CCCAGC	1 cut(s)	2613
PspLI	CGTACG	1 cut(s)	2685
PspN4I	GGNNCC	8 cut(s)	39, 238, 349, 687, 1007, 1999, 2296, 3151
PspPPI	RGGWCCY	4 cut(s)	1006, 1834, 1976, 1997
PsrI	GAACNNNNNNTAC	2 cut(s)	806, 838
PstNI	CAGNNNCTG	1 cut(s)	2888
PsuI	RGATCY	1 cut(s)	1204
PsyI	GACNNNGTC	1 cut(s)	80
RseI	CAYNNNNRTG	3 cut(s)	650, 1185, 1689
SacI	GAGCTC	1 cut(s)	2167
SalI	GTCGAC	2 cut(s)	83, 89
SapI	GCTCTTC	1 cut(s)	383
SaqAI	TTAA	9 cut(s)	746, 1061, 1251, 1839, 1880, 1932, 2076, 2331, 3219
SatI	GCNGC	3 cut(s)	738, 2819, 2870
ScaI	AGTACT	3 cut(s)	1297, 1568, 2767
SchI	GAGTC	6 cut(s)	388, 2031, 2129, 2369, 2548, 2605
SduI	GDGCHC	2 cut(s)	2167, 3015
SfaNI	GCATC	5 cut(s)	522, 544, 1417, 2509, 2849
SfcI	CTRYAG	1 cut(s)	2274
SfoI	GGCGCC	2 cut(s)	238, 349
SgrDI	CGTCGACG	2 cut(s)	83, 89
SmiMI	CAYNNNNRTG	3 cut(s)	650, 1185, 1689
SmlI	CTYRAG	3 cut(s)	1608, 1838, 2357
SmoI	CTYRAG	3 cut(s)	1608, 1838, 2357
SpeI	ACTAGT	1 cut(s)	2339
SphI	GCATGC	1 cut(s)	2060
SspDI	GGCGCC	2 cut(s)	236, 347
SspI	AATATT	2 cut(s)	1159, 3225
SstI	GAGCTC	1 cut(s)	2167
StyI	CCWWGG	6 cut(s)	67, 1014, 1983, 2756, 2939, 3104
TaiI	ACGT	9 cut(s)	84, 90, 183, 828, 1150, 1813, 2113, 2412, 2687
TaqII	GACCGA	3 cut(s)	141, 296, 2583
TatI	WGTACW	4 cut(s)	1295, 1566, 2765, 3073
TfiI	GAWTC	5 cut(s)	443, 491, 1582, 2284, 2578
Tru1I	TTAA	9 cut(s)	746, 1061, 1251, 1839, 1880, 1932, 2076, 2331, 3219
Tru9I	TTAA	9 cut(s)	746, 1061, 1251, 1839, 1880, 1932, 2076, 2331, 3219
TscAI	CASTG	2 cut(s)	652, 3160
TseFI	GTSAC	7 cut(s)	8, 1217, 1726, 2079, 2132, 2377, 2564
TseI	GCWGC	3 cut(s)	737, 2818, 2869
Tsp45I	GTSAC	7 cut(s)	8, 1217, 1726, 2079, 2132, 2377, 2564
TspGWI	ACGGA	4 cut(s)	925, 1679, 2695, 3197
TspRI	CASTG	2 cut(s)	652, 3160
Tth111I	GACNNNGTC	1 cut(s)	80
Van91I	CCANNNNNTGG	2 cut(s)	2702, 2888
Vha464I	CTTAAG	1 cut(s)	1838
VspI	ATTAAT	1 cut(s)	1061
XagI	CCTNNNNNAGG	4 cut(s)	1013, 2205, 2706, 2916
XapI	RAATTY	1 cut(s)	594
XbaI	TCTAGA	2 cut(s)	73, 2144
XceI	RCATGY	3 cut(s)	1652, 1830, 2060
XmiI	GTMKAC	2 cut(s)	84, 90
XmnI	GAANNNNTTC	1 cut(s)	1335
ZraI	GACGTC	2 cut(s)	82, 88
ZrmI	AGTACT	3 cut(s)	1297, 1568, 2767
Zsp2I	ATGCAT	1 cut(s)	537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.