Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Forward (+)

37846635 .. 37855233

Gene Structure 8599 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G019000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3138 bp

ATGCCCCGTCACAGAGACGAAGACGACGACGAGATGGAGCCGGACGAGGACGAGTACGACCCGGACCAAGGTCTAGACGACGTCGACGACGAAGAGGAGGAGGAGGAGGAGGAGGACCGAGGAGGGCGGAGGTCCAGTCAGAAGCGGAGGAGATCGAGCTTCATTGACGACGTGGCCGAGGAGGACGACGAGGAGGAAGAGGAGGACTACGAGGACGAGGATTACGGCGCCTACCGTGGCGGTGGCGGTGGGAGTCGTAAGAAGAGCAAAAAGAAGCCCTCCGGTGCGGATTTTCTTGATCTCGAAGCGGTGGTGGATTCGGATGAGGAGGAAGAGGAGGATGAGGGCGAGGACGACTTTATTGATTCCGGAGCTGATATACCTGAGGAGGGCGATGGTAGAAGGATGCATCATCGCCCATTGATGGATGACCAAGAGCAAGAAGATGTCGAGGCTCTGGAAAGAAGAATTCAGGCAAGATATGCAAAGCAACATCATACAGAAGATACTGAGGAGACCACTGATGTGGATCAGCAAGCTCTTTTACCTTCTGTTTTGGACCCAAAGCTGTGGATGGTGAAATGTGCGATTGGTAAAGAACGAGAGGTAGCTGCTTGCTTAATGCAAAAGTATATTGATAAACCTGAACTGAACATCAGGTCTGTTATTGCTCTTGACCACCTGAAGAACTATATATATGTTGAAGCGGAAAAAGAAGCCCACGTGAGGGAGGCTTGCAAAGGATTGCGCAATATATTTACCCAGAAAATAGCGCTTGTTCCTATTAGAGAAATGACTGATGTTCTTTCCGTTGAAAGCAAGGCAATAGATATTTCTAGGGATACTTGGGTCAGAATGAAGATTGGGATATATAAAGGGGACCTTGCCAAGGTTGTTGATGTGGATAATGTACGACAGAGAGTTACAGTAAAATTAATTCCAAGAATTGATTTACAAGCTCTTGCAAATAAACTGGAGGGCAGAGAAGTTGTCAAAAAGAAAGCGTTTGTGCCTCCGCCACGTTTTATGAATATTGATGAAGCTAGAGAACTGCATATTCGTGTGGAGCGTAAACGAGATCCAATGACGGGTGACTACTTTGAAAACATTGAGAAAATGATGTTTAAAGATGGTTTCTTGTATAAAATAGTATCAATGAAATCAATTAGTACTCAAAACATACATCCCACTTTTGATGAACTGGAAAAGTTTCGGAAACCTGGGGAGAATGGGGAAGGAGATGTTGCCAGTCTGTCAACTTTATTTTCAAACAGAAAGAAAGGACACTTTGTGAAGGGTGATGCAGTCATTGTCATCAAGGGAGACCTAAAAAACTTGAAAGGGCGGGTTGAGAAAGTTGAGGAAGGGACGGTGCATATCAGACCCGACATGAAAGACCTCCCAAAAACTCTTGCCATAAATGAAAAAGAACTTTGCAAGTACTTTGAACCTGGGAATCATGTGAAGGTTGTATCTGGTACTCAAGAAGGTGCAACTGGTATGGTTGTCAAGGTCGATCAGCATGTGCTCATCATTTTATCTGATACAACTAAGGAACATCTCCGTGTGTTTGCTGATGATGTTGTGGAGAGTTCTGAGGTGACATCTGGTATTACCAAAATTGGGGACTATGAGCTTCATGATCTTGTGCTACTGGATAATAATAGCTTTGGTGTAATTATACGTGTTGAAACTGAAGCATGTCAGGTCCTTAAGGGAGTTCCTGAGAGACCTGAGGTTGCCCTTGTCAAATTAAGGGAGATCAAATGCAAGATTGAGAAAAAGCTTAGTGTGCAAGATTGTTTTAAGAACACAATATCTGTGAAAGATGTTGTGAGGGTCGTTGAAGGTCCTTCCAAGGGTAAACAAGGTCCCGTGGAACACATATACAGAGGAGTCTTATTCATTTATGATCGCCATCACATAGAGCATGCGGGCTTTATCTGTGTTAAGTCACATTCTTGTCGTGTTGTAGGAGGATCACGTGCCAATGGTGATAGAAATGGTGACTCCTACTCTAGATTTGATCATCTTCGAGCTCCTCCTCTTATTCCCCAGTCCCCCAGGAGGTTTCCTAGAGGAGGCCCTCCTAATAACTTTGGGGGAAGAAATAGAGGTGGAAGAGGGCATGATGGATTGGTTGGGACTACAGTAAAGATTCGCCAGGGTGCCTATAAGGGTTACCGTGGGCGCGTTGTTGAGGTTAAGGGAACTAGTGTACGGGTGGAACTTGAGTCCCAAATGAAAGTTGTGACAGTTGACCGGAATTGTATTTCTGATAACGTGGCTATTACAACACCGTATCGTGATACATCTAGCTATGGTATGGGAAGTCAGACCCCAATTCATCCTTCTCGAACTCCGCTCCATCCATATATGACGCCAATGAGGGATGCTGGAGCAACGCCTATTCATGATGGCATGAGGACTCCAATGCGTGACCGAGCATGGAATCCGTATGCACCTATGAGTCCAGCAAGGGATAGCTGGGAGGATGGAAACCCTGGTTCTTGGGGTTCTAGCCCACAGTATCAGCCAGGAAGTCCTCCCTCACGGACATACGAAGCTCCAACTCCTGGTTCAGGTTGGGCCAACACTCCTGGTGGGAATTATAGTGAAGCTGGAACACCAAGGGACAGTACTGGTTATGCAAATGCTCCTAGCCCATACTTGCCGTCAACTCCCGGTGGGCAGCCTATGACACCAAACTCAGCATCCTATCTTCCCGGAACTCCTGGAGGGCAGCCAATGACACCAGGGACTGGTGGTCTGGATATGATGTCTCCTGTTATAGGAGGGGACAATGAAGGACCATGGTTCATGCCAGATATACTGGTCAATGTACGGAATTCTGGAGATGAAACTACTGGAATTGTCAGAGAAGTGCTCCTGGATGGCTCATGTAGGGTAGCTCTTGGCTCGGGTGGCAATGGTGATACTGTAACTGTACTTCCAAATGAAATCGAGGTAGTCGTACCAAGGAAGAATGATAAGATCAAAATCATGGGTGGCTCATTACGCGGTGCCACTGGCAAGCTGATTGGTGTCGATGGCACGGATGGAATTGTCAAGGTTGATGACACTCTTGATGTTAAAATATTAGACTTGGCTATTTTGTCAAAACTAGGTCAATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

1045

Amino Acids

116.0

Weight (kDa)

5.22

Isoelectric Point (pI)

42.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5_N	PF11942	98 - 183	3.6e-12	Spt5 transcription elongation factor, acidic N-terminal
Spt5-NGN	PF03439	189 - 269	8.2e-27	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	275 - 404	5e-76	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	432 - 481	4.1e-22	Transcription elongation factor SPT5, second KOW domain
KOWx_SPT5	PF23037	540 - 591	1.4e-17	Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5	PF23291	599 - 652	7e-24	Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5	PF23290	713 - 756	7.2e-18	Transcription elongation factor SPT5, fifth KOW domain
KOW6_SPT51-2	PF23038	940 - 989	8.1e-26	Transcription elongation factor SPT5 homolog 1/2, KOW6 domain
KOW7_SPT5	PF23287	991 - 1042	3.8e-21	Transcription elongation factor SPT5, seventh KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	84
Acc16I	TGCGCA	1 cut(s)	749
AccB1I	GGYRCC	3 cut(s)	227, 2168, 3023
AccB7I	CCANNNNNTGG	2 cut(s)	2576, 2762
AccBSI	CCGCTC	1 cut(s)	2365
AccI	GTMKAC	1 cut(s)	84
AccII	CGCG	2 cut(s)	2193, 3021
AccIII	TCCGGA	1 cut(s)	368
AclWI	GGATC	3 cut(s)	537, 1073, 1987
AcoI	YGGCCR	1 cut(s)	174
AcsI	RAATTY	2 cut(s)	468, 2848
AcuI	CTGAAG	2 cut(s)	704, 1716
AcvI	CACGTG	2 cut(s)	724, 1985
AcyI	GRCGYC	3 cut(s)	81, 228, 2381
AdeI	CACNNNGTG	1 cut(s)	1291
AfeI	AGCGCT	1 cut(s)	774
AflII	CTTAAG	1 cut(s)	1712
AflIII	ACRYGT	1 cut(s)	1684
AgsI	TTSAA	8 cut(s)	704, 815, 1103, 1269, 1339, 1448, 1691, 1847
AhdI	GACNNNNNGTC	1 cut(s)	2766
AhlI	ACTAGT	1 cut(s)	2213
AjiI	CACGTC	1 cut(s)	172
AjuI	GAANNNNNNNTTGG	4 cut(s)	2335, 2367, 2563, 2595
AleI	CACNNNNGTG	1 cut(s)	524
Alw21I	GWGCWC	3 cut(s)	1530, 2041, 2889
Alw26I	GTCTC	5 cut(s)	9, 509, 1317, 1723, 2787
AlwI	GGATC	3 cut(s)	537, 1073, 1987
AlwNI	CAGNNNCTG	1 cut(s)	2762
Ama87I	CYCGRG	1 cut(s)	2920
Aor13HI	TCCGGA	1 cut(s)	368
Aor51HI	AGCGCT	1 cut(s)	774
AoxI	GGCC	3 cut(s)	174, 2083, 2589
ApeKI	GCWGC	3 cut(s)	611, 2692, 2743
ApoI	RAATTY	2 cut(s)	468, 2848
AseI	ATTAAT	1 cut(s)	935
Asp700I	GAANNNNTTC	1 cut(s)	1209
AspLEI	GCGC	4 cut(s)	230, 750, 775, 2193
AsuC2I	CCSGG	3 cut(s)	62, 2685, 2727
AsuHPI	GGTGA	7 cut(s)	589, 1103, 1310, 1612, 2006, 2018, 2945
AvaI	CYCGRG	1 cut(s)	2920
AvaII	GGWCC	9 cut(s)	64, 115, 132, 559, 880, 1708, 1850, 1871, 2810
AxyI	CCTNAGG	2 cut(s)	384, 1734
BaeI	ACNNNNGTAYC	2 cut(s)	834, 867
BanI	GGYRCC	3 cut(s)	227, 2168, 3023
BanII	GRGCYC	1 cut(s)	2041
BarI	GAAGNNNNNNTAC	2 cut(s)	2934, 2966
BbrPI	CACGTG	2 cut(s)	724, 1985
BbsI	GAAGAC	1 cut(s)	27
Bbv12I	GWGCWC	3 cut(s)	1530, 2041, 2889
BbvI	GCAGC	3 cut(s)	598, 2704, 2755
BceAI	ACGGC	2 cut(s)	241, 2659
BciVI	GTATCC	1 cut(s)	835
BclI	TGATCA	1 cut(s)	2026
BcnI	CCSGG	3 cut(s)	62, 2685, 2727
BcoDI	GTCTC	5 cut(s)	9, 509, 1317, 1723, 2787
BcuI	ACTAGT	1 cut(s)	2213
BfmI	CTRYAG	1 cut(s)	2148
BfoI	RGCGCY	2 cut(s)	231, 776
BfrI	CTTAAG	1 cut(s)	1712
BfuI	GTATCC	1 cut(s)	835
BisI	GCNGC	3 cut(s)	612, 2693, 2744
BlsI	GCNGC	3 cut(s)	613, 2694, 2745
BmcAI	AGTACT	3 cut(s)	1171, 1442, 2641
Bme18I	GGWCC	9 cut(s)	64, 115, 132, 559, 880, 1708, 1850, 1871, 2810
BmeRI	GACNNNNNGTC	1 cut(s)	2766
BmeT110I	CYCGRG	1 cut(s)	2920
BmgBI	CACGTC	1 cut(s)	172
BmiI	GGNNCC	7 cut(s)	39, 229, 561, 881, 1873, 2170, 3025
BmrI	ACTGGG	1 cut(s)	2050
BmsI	GCATC	5 cut(s)	396, 418, 1291, 2383, 2723
BmuI	ACTGGG	1 cut(s)	2050
BoxI	GACNNNNGTC	1 cut(s)	69
BpiI	GAAGAC	1 cut(s)	27
BplI	GAGNNNNNCTC	2 cut(s)	2871, 2903
BpmI	CTGGAG	4 cut(s)	995, 2418, 2757, 2874
BpuEI	CTTGAG	2 cut(s)	1467, 2252
BpuMI	CCSGG	3 cut(s)	62, 2685, 2727
BsaAI	YACGTR	3 cut(s)	724, 1685, 1985
BsaBI	GATNNNNATC	3 cut(s)	528, 1917, 2999
BsaHI	GRCGYC	3 cut(s)	81, 228, 2381
BsaI	GGTCTC	3 cut(s)	509, 1317, 1723
BsaWI	WCCGGW	3 cut(s)	281, 368, 2262
BsaXI	ACNNNNNCTCC	2 cut(s)	121, 151
Bse21I	CCTNAGG	2 cut(s)	384, 1734
Bse3DI	GCAATG	1 cut(s)	2935
Bse8I	GATNNNNATC	3 cut(s)	528, 1917, 2999
BseAI	TCCGGA	1 cut(s)	368
BseJI	GATNNNNATC	3 cut(s)	528, 1917, 2999
BseMI	GCAATG	1 cut(s)	2935
BseMII	CTCAG	6 cut(s)	375, 501, 1587, 1716, 1725, 2724
BseXI	GCAGC	3 cut(s)	598, 2704, 2755
BseYI	CCCAGC	1 cut(s)	2487
Bsh1236I	CGCG	2 cut(s)	2193, 3021
BshFI	GGCC	3 cut(s)	176, 2085, 2591
BshNI	GGYRCC	3 cut(s)	227, 2168, 3023
BsiHKAI	GWGCWC	3 cut(s)	1530, 2041, 2889
BsiHKCI	CYCGRG	1 cut(s)	2920
BsiSI	CCGG	7 cut(s)	41, 62, 282, 369, 2263, 2685, 2727
BsmAI	GTCTC	5 cut(s)	9, 509, 1317, 1723, 2787
BsmBI	CGTCTC	1 cut(s)	9
BsnI	GGCC	3 cut(s)	176, 2085, 2591
Bso31I	GGTCTC	3 cut(s)	509, 1317, 1723
BsoBI	CYCGRG	1 cut(s)	2920
Bsp1286I	GDGCHC	3 cut(s)	1530, 2041, 2889
Bsp13I	TCCGGA	1 cut(s)	368
Bsp19I	CCATGG	1 cut(s)	2813
BspANI	GGCC	3 cut(s)	176, 2085, 2591
BspCNI	CTCAG	6 cut(s)	376, 502, 1588, 1717, 1726, 2723
BspEI	TCCGGA	1 cut(s)	368
BspFNI	CGCG	2 cut(s)	2193, 3021
BspHI	TCATGA	2 cut(s)	1639, 2413
BspLI	GGNNCC	7 cut(s)	39, 229, 561, 881, 1873, 2170, 3025
BspPI	GGATC	3 cut(s)	537, 1073, 1987
BspQI	GCTCTTC	1 cut(s)	257
BspT107I	GGYRCC	3 cut(s)	227, 2168, 3023
BspTI	CTTAAG	1 cut(s)	1712
BspTNI	GGTCTC	3 cut(s)	509, 1317, 1723
BsrBI	CCGCTC	1 cut(s)	2365
BsrDI	GCAATG	1 cut(s)	2935
BssNI	GRCGYC	3 cut(s)	81, 228, 2381
BssT1I	CCWWGG	6 cut(s)	67, 888, 1857, 2630, 2813, 2978
Bst6I	CTCTTC	5 cut(s)	87, 192, 257, 327, 2116
BstACI	GRCGYC	3 cut(s)	81, 228, 2381
BstAFI	CTTAAG	1 cut(s)	1712
BstAPI	GCANNNNNTGC	1 cut(s)	482
BstBAI	YACGTR	3 cut(s)	724, 1685, 1985
BstC8I	GCNNGC	6 cut(s)	537, 616, 736, 1932, 1936, 3035
BstDEI	CTNAG	8 cut(s)	384, 510, 1551, 1596, 1725, 1734, 1787, 2710
BstDSI	CCRYGG	4 cut(s)	235, 1875, 2185, 2813
BstEII	GGTNACC	1 cut(s)	2180
BstENI	CCTNNNNNAGG	4 cut(s)	887, 2079, 2580, 2790
BstFNI	CGCG	2 cut(s)	2193, 3021
BstH2I	RGCGCY	2 cut(s)	231, 776
BstHHI	GCGC	4 cut(s)	230, 750, 775, 2193
BstMAI	GTCTC	5 cut(s)	9, 509, 1317, 1723, 2787
BstMWI	GCNNNNNNNGC	4 cut(s)	482, 1792, 2925, 3018
BstNSI	RCATGY	3 cut(s)	1526, 1704, 1934
BstPAI	GACNNNNGTC	1 cut(s)	69
BstPI	GGTNACC	1 cut(s)	2180
BstSFI	CTRYAG	1 cut(s)	2148
BstUI	CGCG	2 cut(s)	2193, 3021
BstV1I	GCAGC	3 cut(s)	598, 2704, 2755
BstV2I	GAAGAC	1 cut(s)	27
BstX2I	RGATCY	1 cut(s)	1078
BstXI	CCANNNNNNTGG	2 cut(s)	526, 570
BstYI	RGATCY	1 cut(s)	1078
Bsu36I	CCTNAGG	2 cut(s)	384, 1734
BsuI	GTATCC	1 cut(s)	835
BsuRI	GGCC	3 cut(s)	176, 2085, 2591
BtgI	CCRYGG	4 cut(s)	235, 1875, 2185, 2813
BtgZI	GCGATG	2 cut(s)	398, 408
BtrI	CACGTC	1 cut(s)	172
BtsIMutI	CAGTG	2 cut(s)	519, 3027
Cac8I	GCNNGC	6 cut(s)	537, 616, 736, 1932, 1936, 3035
CaiI	CAGNNNCTG	1 cut(s)	2762
CciI	TCATGA	2 cut(s)	1639, 2413
CfoI	GCGC	4 cut(s)	230, 750, 775, 2193
CseI	GACGC	1 cut(s)	2389
DdeI	CTNAG	8 cut(s)	384, 510, 1551, 1596, 1725, 1734, 1787, 2710
DinI	GGCGCC	1 cut(s)	229
DraI	TTTAAA	1 cut(s)	1126
DraIII	CACNNNGTG	1 cut(s)	1291
DriI	GACNNNNNGTC	1 cut(s)	2766
EaeI	YGGCCR	1 cut(s)	174
Eam1104I	CTCTTC	5 cut(s)	87, 192, 257, 327, 2116
Eam1105I	GACNNNNNGTC	1 cut(s)	2766
EarI	CTCTTC	5 cut(s)	87, 192, 257, 327, 2116
EciI	GGCGGA	2 cut(s)	142, 1005
Ecl136II	GAGCTC	1 cut(s)	2039
Eco130I	CCWWGG	6 cut(s)	67, 888, 1857, 2630, 2813, 2978
Eco24I	GRGCYC	1 cut(s)	2041
Eco31I	GGTCTC	3 cut(s)	509, 1317, 1723
Eco47I	GGWCC	9 cut(s)	64, 115, 132, 559, 880, 1708, 1850, 1871, 2810
Eco47III	AGCGCT	1 cut(s)	774
Eco53kI	GAGCTC	1 cut(s)	2039
Eco57I	CTGAAG	2 cut(s)	704, 1716
Eco72I	CACGTG	2 cut(s)	724, 1985
Eco81I	CCTNAGG	2 cut(s)	384, 1734
Eco88I	CYCGRG	1 cut(s)	2920
Eco91I	GGTNACC	1 cut(s)	2180
EcoICRI	GAGCTC	1 cut(s)	2039
EcoNI	CCTNNNNNAGG	4 cut(s)	887, 2079, 2580, 2790
EcoO109I	RGGNCCY	5 cut(s)	880, 1708, 1850, 1871, 2084
EcoO65I	GGTNACC	1 cut(s)	2180
EcoRI	GAATTC	2 cut(s)	468, 2848
EcoT14I	CCWWGG	6 cut(s)	67, 888, 1857, 2630, 2813, 2978
EcoT22I	ATGCAT	1 cut(s)	411
EcoT38I	GRGCYC	1 cut(s)	2041
EgeI	GGCGCC	1 cut(s)	229
EheI	GGCGCC	1 cut(s)	229
ErhI	CCWWGG	6 cut(s)	67, 888, 1857, 2630, 2813, 2978
Esp3I	CGTCTC	1 cut(s)	9
FalI	AAGNNNNNCTT	2 cut(s)	867, 899
FauI	CCCGC	2 cut(s)	1338, 1927
FbaI	TGATCA	1 cut(s)	2026
FblI	GTMKAC	1 cut(s)	84
Fnu4HI	GCNGC	3 cut(s)	612, 2693, 2744
FriOI	GRGCYC	1 cut(s)	2041
Fsp4HI	GCNGC	3 cut(s)	612, 2693, 2744
FspI	TGCGCA	1 cut(s)	749
GlaI	GCGC	4 cut(s)	229, 749, 774, 2192
GluI	GCNGC	3 cut(s)	612, 2693, 2744
GsaI	CCCAGC	1 cut(s)	2491
GsuI	CTGGAG	4 cut(s)	995, 2418, 2757, 2874
HaeII	RGCGCY	2 cut(s)	231, 776
HaeIII	GGCC	3 cut(s)	176, 2085, 2591
HapII	CCGG	7 cut(s)	41, 62, 282, 369, 2263, 2685, 2727
HgaI	GACGC	1 cut(s)	2389
HhaI	GCGC	4 cut(s)	230, 750, 775, 2193
Hin1I	GRCGYC	3 cut(s)	81, 228, 2381
Hin6I	GCGC	4 cut(s)	228, 748, 773, 2191
HinP1I	GCGC	4 cut(s)	228, 748, 773, 2191
HincII	GTYRAC	4 cut(s)	85, 1257, 2260, 2679
HindII	GTYRAC	4 cut(s)	85, 1257, 2260, 2679
HindIII	AAGCTT	1 cut(s)	1784
HpaII	CCGG	7 cut(s)	41, 62, 282, 369, 2263, 2685, 2727
HphI	GGTGA	7 cut(s)	589, 1103, 1310, 1612, 2006, 2018, 2945
Hpy166II	GTNNAC	7 cut(s)	85, 1073, 1257, 1865, 2219, 2260, 2679
Hpy8I	GTNNAC	7 cut(s)	85, 1073, 1257, 1865, 2219, 2260, 2679
Hpy99I	CGWCG	8 cut(s)	29, 32, 83, 86, 89, 92, 173, 191
HpyCH4IV	ACGT	7 cut(s)	81, 171, 723, 1021, 1684, 1984, 2283
HpyF10VI	GCNNNNNNNGC	4 cut(s)	482, 1792, 2925, 3018
HpyF3I	CTNAG	8 cut(s)	384, 510, 1551, 1596, 1725, 1734, 1787, 2710
HpySE526I	ACGT	7 cut(s)	81, 171, 723, 1021, 1684, 1984, 2283
Hsp92I	GRCGYC	3 cut(s)	81, 228, 2381
HspAI	GCGC	4 cut(s)	228, 748, 773, 2191
KasI	GGCGCC	1 cut(s)	227
Kpn2I	TCCGGA	1 cut(s)	368
Ksp22I	TGATCA	1 cut(s)	2026
LguI	GCTCTTC	1 cut(s)	257
LmnI	GCTCC	9 cut(s)	37, 371, 1066, 2044, 2370, 2399, 2572, 2662, 2892
Lsp1109I	GCAGC	3 cut(s)	598, 2704, 2755
LweI	GCATC	5 cut(s)	396, 418, 1291, 2383, 2723
MaeII	ACGT	7 cut(s)	81, 171, 723, 1021, 1684, 1984, 2283
MbiI	CCGCTC	1 cut(s)	2365
MflI	RGATCY	1 cut(s)	1078
MhlI	GDGCHC	3 cut(s)	1530, 2041, 2889
Mly113I	GGCGCC	1 cut(s)	228
MlyI	GAGTC	6 cut(s)	262, 1905, 2003, 2243, 2422, 2479
MmeI	TCCRAC	1 cut(s)	2594
Mph1103I	ATGCAT	1 cut(s)	411
MroI	TCCGGA	1 cut(s)	368
MroXI	GAANNNNTTC	1 cut(s)	1209
MseI	TTAA	9 cut(s)	620, 935, 1125, 1713, 1754, 1806, 1950, 2205, 3093
MslI	CAYNNNNRTG	3 cut(s)	524, 1059, 1563
MspCI	CTTAAG	1 cut(s)	1712
MspI	CCGG	7 cut(s)	41, 62, 282, 369, 2263, 2685, 2727
MvnI	CGCG	2 cut(s)	2193, 3021
MwoI	GCNNNNNNNGC	4 cut(s)	482, 1792, 2925, 3018
NarI	GGCGCC	1 cut(s)	228
NciI	CCSGG	3 cut(s)	62, 2685, 2727
NcoI	CCATGG	1 cut(s)	2813
NlaIV	GGNNCC	7 cut(s)	39, 229, 561, 881, 1873, 2170, 3025
NmeAIII	GCCGAG	1 cut(s)	202
NmuCI	GTSAC	7 cut(s)	8, 1091, 1600, 1953, 2006, 2251, 2438
NsbI	TGCGCA	1 cut(s)	749
NsiI	ATGCAT	1 cut(s)	411
NspI	RCATGY	3 cut(s)	1526, 1704, 1934
OliI	CACNNNNGTG	1 cut(s)	524
PaeI	GCATGC	1 cut(s)	1934
PagI	TCATGA	2 cut(s)	1639, 2413
PciSI	GCTCTTC	1 cut(s)	257
PcsI	WCGNNNNNNNCGW	6 cut(s)	24, 84, 87, 174, 1066, 2970
PdmI	GAANNNNTTC	1 cut(s)	1209
PfeI	GAWTC	5 cut(s)	317, 365, 1456, 2158, 2452
PflFI	GACNNNGTC	1 cut(s)	80
PflMI	CCANNNNNTGG	2 cut(s)	2576, 2762
PfoI	TCCNGGA	3 cut(s)	2725, 2734, 2889
PkrI	GCNGC	3 cut(s)	613, 2694, 2745
PleI	GAGTC	6 cut(s)	261, 1904, 2003, 2242, 2422, 2478
PluTI	GGCGCC	1 cut(s)	231
PmaCI	CACGTG	2 cut(s)	724, 1985
PmlI	CACGTG	2 cut(s)	724, 1985
PpsI	GAGTC	6 cut(s)	261, 1904, 2003, 2242, 2422, 2478
Ppu21I	YACGTR	3 cut(s)	724, 1685, 1985
PpuMI	RGGWCCY	4 cut(s)	880, 1708, 1850, 1871
PshAI	GACNNNNGTC	1 cut(s)	69
PshBI	ATTAAT	1 cut(s)	935
Psp124BI	GAGCTC	1 cut(s)	2041
Psp5II	RGGWCCY	4 cut(s)	880, 1708, 1850, 1871
PspCI	CACGTG	2 cut(s)	724, 1985
PspEI	GGTNACC	1 cut(s)	2180
PspFI	CCCAGC	1 cut(s)	2487
PspN4I	GGNNCC	7 cut(s)	39, 229, 561, 881, 1873, 2170, 3025
PspPPI	RGGWCCY	4 cut(s)	880, 1708, 1850, 1871
PstNI	CAGNNNCTG	1 cut(s)	2762
PsuI	RGATCY	1 cut(s)	1078
PsyI	GACNNNGTC	1 cut(s)	80
RseI	CAYNNNNRTG	3 cut(s)	524, 1059, 1563
SacI	GAGCTC	1 cut(s)	2041
SalI	GTCGAC	1 cut(s)	83
SapI	GCTCTTC	1 cut(s)	257
SaqAI	TTAA	9 cut(s)	620, 935, 1125, 1713, 1754, 1806, 1950, 2205, 3093
SatI	GCNGC	3 cut(s)	612, 2693, 2744
ScaI	AGTACT	3 cut(s)	1171, 1442, 2641
SchI	GAGTC	6 cut(s)	262, 1905, 2003, 2243, 2422, 2479
SduI	GDGCHC	3 cut(s)	1530, 2041, 2889
SfaNI	GCATC	5 cut(s)	396, 418, 1291, 2383, 2723
SfcI	CTRYAG	1 cut(s)	2148
SfoI	GGCGCC	1 cut(s)	229
SgrDI	CGTCGACG	1 cut(s)	83
SinI	GGWCC	9 cut(s)	64, 115, 132, 559, 880, 1708, 1850, 1871, 2810
SmiMI	CAYNNNNRTG	3 cut(s)	524, 1059, 1563
SmlI	CTYRAG	3 cut(s)	1482, 1712, 2231
SmoI	CTYRAG	3 cut(s)	1482, 1712, 2231
SpeI	ACTAGT	1 cut(s)	2213
SphI	GCATGC	1 cut(s)	1934
SspDI	GGCGCC	1 cut(s)	227
SspI	AATATT	2 cut(s)	1033, 3099
SstI	GAGCTC	1 cut(s)	2041
StyI	CCWWGG	6 cut(s)	67, 888, 1857, 2630, 2813, 2978
TaiI	ACGT	7 cut(s)	84, 174, 726, 1024, 1687, 1987, 2286
TaqI	TCGA	9 cut(s)	84, 155, 303, 450, 1515, 2035, 2356, 2964, 3048
TaqII	GACCGA	2 cut(s)	132, 2457
TatI	WGTACW	4 cut(s)	1169, 1440, 2639, 2947
TfiI	GAWTC	5 cut(s)	317, 365, 1456, 2158, 2452
Tru1I	TTAA	9 cut(s)	620, 935, 1125, 1713, 1754, 1806, 1950, 2205, 3093
Tru9I	TTAA	9 cut(s)	620, 935, 1125, 1713, 1754, 1806, 1950, 2205, 3093
TscAI	CASTG	2 cut(s)	526, 3034
TseFI	GTSAC	7 cut(s)	8, 1091, 1600, 1953, 2006, 2251, 2438
TseI	GCWGC	3 cut(s)	611, 2692, 2743
Tsp45I	GTSAC	7 cut(s)	8, 1091, 1600, 1953, 2006, 2251, 2438
TspGWI	ACGGA	6 cut(s)	799, 1553, 2445, 2569, 2860, 3071
TspRI	CASTG	2 cut(s)	526, 3034
Tth111I	GACNNNGTC	1 cut(s)	80
Van91I	CCANNNNNTGG	2 cut(s)	2576, 2762
Vha464I	CTTAAG	1 cut(s)	1712
VpaK11BI	GGWCC	9 cut(s)	64, 115, 132, 559, 880, 1708, 1850, 1871, 2810
VspI	ATTAAT	1 cut(s)	935
XagI	CCTNNNNNAGG	4 cut(s)	887, 2079, 2580, 2790
XapI	RAATTY	2 cut(s)	468, 2848
XbaI	TCTAGA	2 cut(s)	73, 2018
XceI	RCATGY	3 cut(s)	1526, 1704, 1934
XmiI	GTMKAC	1 cut(s)	84
XmnI	GAANNNNTTC	1 cut(s)	1209
ZraI	GACGTC	1 cut(s)	82
ZrmI	AGTACT	3 cut(s)	1171, 1442, 2641
Zsp2I	ATGCAT	1 cut(s)	411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.