Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Reverse (-)

12951606 .. 12959927

Gene Structure 8322 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g14770.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3126 bp

ATGCCGCGTCACAGAGACGAAGACGATGACGACATGGAGCCGGAGGAGGACGAATATGACCCCGACCAAGGCCTAGACGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAGGAGGAACGAGGACGGAGGTCCAGCCAGAAGCGGAGGAGATCGAGCTTCATTGACGACGTGGCGGAGGAGGACGACGAGGAGGAAGAGGAAGACGACGACGACGAGGAATACGGCGCCTATCGCGGTGGCGGTGGGAGGAATAAGAAGAAGAAGAAGAAGCCCTCCGGTGCGGACTTTCTTGATCTTGAAGCAGAAGTCGATTCGGACGAGGAAGAAGAGGAGGAAGAGGGCGACGACGACTTTATTGAAGATGGAGCTGATATACCCGAGGAGGGAGGTCGTAGAAGGATGCATCGTCCCCCATTGTTGGATGACCAGCCAGAAGATGTTGAGGATCTGGAAAGAAGAATTCAGGAAAGGTATGCAAGGCAACATCATACAGAATACGCCGAGGAGACCACTGATGTGGATCAGCAAGCTCTTTTACCTTCTGTTTTGGACCCAAAGCTGTGGATGGTGAAATGTGCGATCGGTAAAGAGCGAGAGGTTGCTGCTTGCTTAATGCAAAAGTATATTGACAAACCTGAACTGAACATCAGGTCTGCTATTGCTCTTGACCACCTGAAGAACTATATATACGTTGAAGCCGAAAAAGAAGCCCATGTGAGGGAGGCTTGCAAAGGAATGCGCAATATATTTTTGGCGAAAATATCGCTTGTTCCTATCAGAGAAATGACTGATGTTCTTTCTGTTGAAAGCAAGGCAATTGAAATTTCGAGGGATACTTGGGTCAGAATGAAGATTGGGATATATAAAGGGGACCTTGCCAAGGTTGTCGATGTGGATAATGTACGACAAAGAGTTACAGTAAAATTAATTCCAAGAATTGATTTACAAGCTCTTGCAAATAAACTGGAGGGCAGAGAAGTGGTGAAAAAGAAAGCGTTTGTTCCTCCGCCACGTTTTATGAATATTGATGAAGCTAGGGAACTGCATATTCGTGTGGAGCGTAAACGAGATCCAATGACGGGTGACTACTTTGAAAACATTGAGAAAATGATGTTTAAAGAGGGCTTCTTGTATAAAATAGTATCAATGAAATCAATTAGTACTCAAAACATACATCCCACTTTTGATGAACTGGAAAAGTTCCGGAAACCTGGGGAGAATGGGGAAGGAGATATAGCCAGTCTGTCAACTTTATTTTCAAACAGAAAGAAAGGACACTTTGTGAAGGGTGATGCAGTCATCATAATCAAGGGAGACCTAAAGAACTTGAAAGGGCGGGTTGAGAAAGTTGAGGATGGGACTGTGCATATCAGACCTGAAATGAAGGACCTCCCTAAAACTCTTGCCGTAAATGAAAAAGATCTTTGCAAGTACTTTGAAGCTGGGAATCATGTGAAGGTTGTATCTGGTACTCAAGAAGGTGCAACTGGCATGGTTGTCAAGGTTGAGCAGCATGTGCTCATCATTTTATCTGATACAACTAAGGAACATCTCCGTGTATTTGCTGATGATGTTGTGGAGAGTTCTGAAGTGACATCTGGGATCACCAAAATTGGGGACTATGAGCTTCATGATCTTGTGCTACTGGATAATAATAGCTTTGGTGTAATTATACGTGTTGAAACTGAAGCATGTCAGGTCCTTAAGGGAGTTCCTGAGAGACCTGAGGTTGCCCTTATTAAATTAAGAGAGATCAAATGCAAGATTGACAAAAAGCTTAGTGTGCAAGATTGTTTTAAGAACACAATATCTGTGAAAGATGTTGTAAGGGTCGTCGAAGGTCCTTCCAAGGGGAAACAAGGTCCCGTGGAACACATATACAGAGGAGTCTTATTCATTTATGATCGCCATCACATAGAGCATGCAGGCTTTATCTGTGTTAAGTCACATTCTTGTCGTGTTGTAGGAGGATCACGTGCCAATGGAGATAGAAATGGTGACTCCTACTCTAGATTTGATCATCTTCGAGCTCCTCCTGCGATTCCCCCGTCCCCAAGGAGGTTTACTAGAGGAGGCCCTCCCAACAATTTTGGGGGAAGAAATAGAGGTGGAAGAGGGCATGATGGATTGGTTGGGACTACAGTAAAGATTCGCCAGGGTGCCTATAAGGGCTACCGTGGGCGCGTTGTTGAGGTAAAGGGAACTAGTGTACGGGTGGAACTCGAGTCCCAAATGAAAGTTGTTACAGTTGACCGGAATTGTATTTCTGACAACGTGGCTATTACAACACCTTATCGTGATACGTCTAGCTATGGTATGGGAAGTCAGACCCCGATTCATCCTTCTCGAACTCCGCTACATCCTTATATGACTCCAATGAGGGATGCTGGAGCAACGCCCATTCATGATGGAATGAGGACTCCGATGCGTGATCGAGCATGGAATCCGTATGCACCTATGAGCCCAGCAAGGGATAGCTGGGAGGATGGAAACCCTGGTTCTTGGGGCACTAGTCCACAGTATCAGCCAGGAAGTCCTCCTTCACGGACATATGAAGCTCCAACTCCTGGTTCAGGTTGGGCCAGCACTCCTGGTGGAAACTATAGTGAAGCTGGAACACCAAGGGACAGTACTGGTTATGCAAATGCTCCTAGCCCGTACTTGCCGTCGACTCCTGGTGGGCAGCCTATGACACCAAACTCAGCATCCTATCTTCCTGGAACTCCGGGAGGGCAGCCAATGACACCAGGGACTGGTCTGGATATGATGTCTCCTGTTATAGGTGGGGACAACGAAGGACCATGGTTCATGCCAGATATATTGGTCAATGTACGCCATTCTGGAGAGGAAACTACTGGGATTGTCAGAGAAGTGCTCCTGGATGGCTCATGTAGGGTAGCTCTTGGATCGGGTGGCAATGGTGAAACTGTAACTGTACATCCAAATGAAATGGAGGTAGTCGCACCAAGGAAGAATGATAAGATCAAAATCATGGGTGGCTCATTGCGTGGTGCCACCGGCAAGCTGATAGGAGTTGATGGCACGGATGGAATTGTCAAGGTTGATGACACTCTTGATGTTAAAATATTAGACTTAGCTATTTTGTCAAAACTGGCTCAATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

1042

Amino Acids

115.95

Weight (kDa)

5.24

Isoelectric Point (pI)

46.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5_N	PF11942	40 - 101	5.5e-07	Spt5 transcription elongation factor, acidic N-terminal
Spt5-NGN	PF03439	107 - 187	2.8e-26	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	193 - 322	6.6e-75	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	350 - 399	4.5e-21	Transcription elongation factor SPT5, second KOW domain
KOWx_SPT5	PF23037	458 - 509	5e-17	Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5	PF23291	517 - 570	6.3e-24	Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5	PF23290	631 - 674	6.5e-18	Transcription elongation factor SPT5, fifth KOW domain
KOW6_SPT51-2	PF23038	857 - 906	1.7e-26	Transcription elongation factor SPT5 homolog 1/2, KOW6 domain
KOW7_SPT5	PF23287	908 - 959	3.4e-21	Transcription elongation factor SPT5, seventh KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	740
AccB1I	GGYRCC	3 cut(s)	224, 2159, 3011
AccB7I	CCANNNNNTGG	2 cut(s)	2567, 2753
AccI	GTMKAC	1 cut(s)	2669
AccII	CGCG	3 cut(s)	7, 234, 2184
AccIII	TCCGGA	1 cut(s)	1203
AciI	CCGC	9 cut(s)	5, 142, 173, 234, 240, 281, 1007, 1336, 2354
AclWI	GGATC	6 cut(s)	453, 528, 1064, 1610, 1978, 2914
AcsI	RAATTY	2 cut(s)	459, 822
AcuI	CTGAAG	3 cut(s)	695, 1608, 1707
AcvI	CACGTG	1 cut(s)	1976
AcyI	GRCGYC	1 cut(s)	225
AdeI	CACNNNGTG	1 cut(s)	1282
AfaI	GTAC	9 cut(s)	903, 1162, 1433, 1471, 2211, 2632, 2660, 2832, 2937
AflII	CTTAAG	1 cut(s)	1703
AflIII	ACRYGT	1 cut(s)	1675
AhlI	ACTAGT	2 cut(s)	2204, 2510
AjiI	CACGTC	1 cut(s)	169
AjuI	GAANNNNNNNTTGG	2 cut(s)	2554, 2586
AleI	CACNNNNGTG	1 cut(s)	515
Alw21I	GWGCWC	3 cut(s)	1521, 2032, 2877
Alw26I	GTCTC	5 cut(s)	9, 500, 1308, 1714, 2775
AlwI	GGATC	6 cut(s)	453, 528, 1064, 1610, 1978, 2914
AlwNI	CAGNNNCTG	1 cut(s)	2753
Ama87I	CYCGRG	2 cut(s)	377, 2222
Aor13HI	TCCGGA	1 cut(s)	1203
AoxI	GGCC	3 cut(s)	70, 2074, 2580
ApeKI	GCWGC	4 cut(s)	602, 1510, 2683, 2734
ApoI	RAATTY	2 cut(s)	459, 822
AseI	ATTAAT	1 cut(s)	926
AspLEI	GCGC	3 cut(s)	227, 741, 2184
AsuC2I	CCSGG	1 cut(s)	2727
AsuHPI	GGTGA	7 cut(s)	580, 994, 1094, 1301, 1597, 2009, 2933
AvaI	CYCGRG	2 cut(s)	377, 2222
AvaII	GGWCC	8 cut(s)	129, 550, 871, 1387, 1699, 1841, 1862, 2798
AxyI	CCTNAGG	1 cut(s)	1725
BaeGI	GKGCMC	1 cut(s)	2510
BaeI	ACNNNNGTAYC	2 cut(s)	825, 858
BanI	GGYRCC	3 cut(s)	224, 2159, 3011
BanII	GRGCYC	2 cut(s)	2032, 2465
BbrPI	CACGTG	1 cut(s)	1976
BbsI	GAAGAC	2 cut(s)	27, 207
Bbv12I	GWGCWC	3 cut(s)	1521, 2032, 2877
BbvI	GCAGC	4 cut(s)	589, 1522, 2695, 2746
BceAI	ACGGC	3 cut(s)	238, 1391, 2650
BciVI	GTATCC	1 cut(s)	826
BclI	TGATCA	1 cut(s)	2017
BcnI	CCSGG	1 cut(s)	2727
BcoDI	GTCTC	5 cut(s)	9, 500, 1308, 1714, 2775
BcuI	ACTAGT	2 cut(s)	2204, 2510
BfaI	CTAG	8 cut(s)	74, 1035, 2010, 2067, 2205, 2307, 2511, 2652
BfmI	CTRYAG	2 cut(s)	2139, 2602
BfoI	RGCGCY	1 cut(s)	228
BfrI	CTTAAG	1 cut(s)	1703
BfuI	GTATCC	1 cut(s)	826
BglII	AGATCT	1 cut(s)	1420
BisI	GCNGC	5 cut(s)	5, 603, 1511, 2684, 2735
BlsI	GCNGC	5 cut(s)	6, 604, 1512, 2685, 2736
BmcAI	AGTACT	3 cut(s)	1162, 1433, 2632
Bme18I	GGWCC	8 cut(s)	129, 550, 871, 1387, 1699, 1841, 1862, 2798
BmeT110I	CYCGRG	2 cut(s)	377, 2222
BmgBI	CACGTC	1 cut(s)	169
BmiI	GGNNCC	7 cut(s)	39, 226, 552, 872, 1864, 2161, 3013
BmrI	ACTGGG	1 cut(s)	2865
BmsI	GCATC	6 cut(s)	390, 412, 1282, 2374, 2415, 2714
BmuI	ACTGGG	1 cut(s)	2865
BoxI	GACNNNNGTC	1 cut(s)	127
BpiI	GAAGAC	2 cut(s)	27, 207
BplI	GAGNNNNNCTC	2 cut(s)	2859, 2891
BpmI	CTGGAG	3 cut(s)	986, 2409, 2862
BpuEI	CTTGAG	1 cut(s)	1458
BpuMI	CCSGG	1 cut(s)	2727
BsaAI	YACGTR	2 cut(s)	1676, 1976
BsaBI	GATNNNNATC	3 cut(s)	519, 1908, 2987
BsaHI	GRCGYC	1 cut(s)	225
BsaI	GGTCTC	3 cut(s)	500, 1308, 1714
BsaWI	WCCGGW	3 cut(s)	275, 1203, 2253
Bse118I	RCCGGY	1 cut(s)	3017
Bse1I	ACTGG	9 cut(s)	969, 1197, 1239, 1492, 1650, 2638, 2758, 2860, 3117
Bse21I	CCTNAGG	1 cut(s)	1725
Bse3DI	GCAATG	2 cut(s)	2923, 3002
Bse8I	GATNNNNATC	3 cut(s)	519, 1908, 2987
BseAI	TCCGGA	1 cut(s)	1203
BseJI	GATNNNNATC	3 cut(s)	519, 1908, 2987
BseMI	GCAATG	2 cut(s)	2923, 3002
BseMII	CTCAG	3 cut(s)	1707, 1716, 2715
BseNI	ACTGG	9 cut(s)	969, 1197, 1239, 1492, 1650, 2638, 2758, 2860, 3117
BseSI	GKGCMC	1 cut(s)	2510
BseXI	GCAGC	4 cut(s)	589, 1522, 2695, 2746
BseYI	CCCAGC	3 cut(s)	1442, 2464, 2478
Bsh1236I	CGCG	3 cut(s)	7, 234, 2184
Bsh1285I	CGRYCG	1 cut(s)	582
BshFI	GGCC	3 cut(s)	72, 2076, 2582
BshNI	GGYRCC	3 cut(s)	224, 2159, 3011
BsiEI	CGRYCG	1 cut(s)	582
BsiHKAI	GWGCWC	3 cut(s)	1521, 2032, 2877
BsiHKCI	CYCGRG	2 cut(s)	377, 2222
BsiSI	CCGG	6 cut(s)	41, 276, 1204, 2254, 2726, 3018
BsmAI	GTCTC	5 cut(s)	9, 500, 1308, 1714, 2775
BsmBI	CGTCTC	1 cut(s)	9
BsmI	GAATGC	1 cut(s)	741
BsnI	GGCC	3 cut(s)	72, 2076, 2582
Bso31I	GGTCTC	3 cut(s)	500, 1308, 1714
BsoBI	CYCGRG	2 cut(s)	377, 2222
Bsp1286I	GDGCHC	5 cut(s)	1521, 2032, 2465, 2510, 2877
Bsp13I	TCCGGA	1 cut(s)	1203
Bsp1407I	TGTACA	1 cut(s)	2935
Bsp19I	CCATGG	1 cut(s)	2801
BspACI	CCGC	9 cut(s)	5, 142, 173, 234, 240, 281, 1007, 1336, 2354
BspANI	GGCC	3 cut(s)	72, 2076, 2582
BspCNI	CTCAG	3 cut(s)	1708, 1717, 2714
BspEI	TCCGGA	1 cut(s)	1203
BspFNI	CGCG	3 cut(s)	7, 234, 2184
BspHI	TCATGA	2 cut(s)	1630, 2404
BspLI	GGNNCC	7 cut(s)	39, 226, 552, 872, 1864, 2161, 3013
BspPI	GGATC	6 cut(s)	453, 528, 1064, 1610, 1978, 2914
BspT107I	GGYRCC	3 cut(s)	224, 2159, 3011
BspTI	CTTAAG	1 cut(s)	1703
BspTNI	GGTCTC	3 cut(s)	500, 1308, 1714
BsrDI	GCAATG	2 cut(s)	2923, 3002
BsrFI	RCCGGY	1 cut(s)	3017
BsrGI	TGTACA	1 cut(s)	2935
BsrI	ACTGG	9 cut(s)	969, 1197, 1239, 1492, 1650, 2638, 2758, 2860, 3117
BssAI	RCCGGY	1 cut(s)	3017
BssNI	GRCGYC	1 cut(s)	225
BssT1I	CCWWGG	7 cut(s)	67, 879, 1848, 2054, 2621, 2801, 2966
Bst4CI	ACNGT	9 cut(s)	919, 1363, 2143, 2177, 2248, 2520, 2630, 2929, 2935
Bst6I	CTCTTC	5 cut(s)	90, 189, 321, 330, 2107
BstACI	GRCGYC	1 cut(s)	225
BstAFI	CTTAAG	1 cut(s)	1703
BstAPI	GCANNNNNTGC	1 cut(s)	1516
BstAUI	TGTACA	1 cut(s)	2935
BstBAI	YACGTR	2 cut(s)	1676, 1976
BstC8I	GCNNGC	7 cut(s)	528, 607, 727, 1923, 1927, 2584, 3023
BstDEI	CTNAG	6 cut(s)	1542, 1716, 1725, 1778, 2701, 3094
BstDSI	CCRYGG	3 cut(s)	1866, 2176, 2801
BstENI	CCTNNNNNAGG	4 cut(s)	77, 878, 2571, 2778
BstFNI	CGCG	3 cut(s)	7, 234, 2184
BstH2I	RGCGCY	1 cut(s)	228
BstHHI	GCGC	3 cut(s)	227, 741, 2184
BstMAI	GTCTC	5 cut(s)	9, 500, 1308, 1714, 2775
BstMCI	CGRYCG	1 cut(s)	582
BstMWI	GCNNNNNNNGC	4 cut(s)	231, 1516, 1783, 2036
BstNSI	RCATGY	3 cut(s)	1517, 1695, 1925
BstPAI	GACNNNNGTC	1 cut(s)	127
BstSFI	CTRYAG	2 cut(s)	2139, 2602
BstSLI	GKGCMC	1 cut(s)	2510
BstUI	CGCG	3 cut(s)	7, 234, 2184
BstV1I	GCAGC	4 cut(s)	589, 1522, 2695, 2746
BstV2I	GAAGAC	2 cut(s)	27, 207
BstX2I	RGATCY	3 cut(s)	445, 1069, 1420
BstXI	CCANNNNNNTGG	2 cut(s)	517, 561
BstYI	RGATCY	3 cut(s)	445, 1069, 1420
Bsu36I	CCTNAGG	1 cut(s)	1725
BsuI	GTATCC	1 cut(s)	826
BsuRI	GGCC	3 cut(s)	72, 2076, 2582
BtgI	CCRYGG	3 cut(s)	1866, 2176, 2801
BtrI	CACGTC	1 cut(s)	169
BtsIMutI	CAGTG	1 cut(s)	510
Cac8I	GCNNGC	7 cut(s)	528, 607, 727, 1923, 1927, 2584, 3023
CaiI	CAGNNNCTG	1 cut(s)	2753
CciI	TCATGA	2 cut(s)	1630, 2404
CfoI	GCGC	3 cut(s)	227, 741, 2184
Cfr10I	RCCGGY	1 cut(s)	3017
Csp6I	GTAC	9 cut(s)	902, 1161, 1432, 1470, 2210, 2631, 2659, 2831, 2936
CviQI	GTAC	9 cut(s)	902, 1161, 1432, 1470, 2210, 2631, 2659, 2831, 2936
DdeI	CTNAG	6 cut(s)	1542, 1716, 1725, 1778, 2701, 3094
DinI	GGCGCC	1 cut(s)	226
DraI	TTTAAA	1 cut(s)	1117
DraIII	CACNNNGTG	1 cut(s)	1282
Eam1104I	CTCTTC	5 cut(s)	90, 189, 321, 330, 2107
EarI	CTCTTC	5 cut(s)	90, 189, 321, 330, 2107
EciI	GGCGGA	2 cut(s)	188, 996
Ecl136II	GAGCTC	1 cut(s)	2030
Eco130I	CCWWGG	7 cut(s)	67, 879, 1848, 2054, 2621, 2801, 2966
Eco147I	AGGCCT	1 cut(s)	72
Eco24I	GRGCYC	2 cut(s)	2032, 2465
Eco31I	GGTCTC	3 cut(s)	500, 1308, 1714
Eco47I	GGWCC	8 cut(s)	129, 550, 871, 1387, 1699, 1841, 1862, 2798
Eco53kI	GAGCTC	1 cut(s)	2030
Eco57I	CTGAAG	3 cut(s)	695, 1608, 1707
Eco72I	CACGTG	1 cut(s)	1976
Eco81I	CCTNAGG	1 cut(s)	1725
Eco88I	CYCGRG	2 cut(s)	377, 2222
EcoICRI	GAGCTC	1 cut(s)	2030
EcoNI	CCTNNNNNAGG	4 cut(s)	77, 878, 2571, 2778
EcoO109I	RGGNCCY	6 cut(s)	871, 1387, 1699, 1841, 1862, 2075
EcoRI	GAATTC	1 cut(s)	459
EcoT14I	CCWWGG	7 cut(s)	67, 879, 1848, 2054, 2621, 2801, 2966
EcoT22I	ATGCAT	1 cut(s)	405
EcoT38I	GRGCYC	2 cut(s)	2032, 2465
EgeI	GGCGCC	1 cut(s)	226
EheI	GGCGCC	1 cut(s)	226
ErhI	CCWWGG	7 cut(s)	67, 879, 1848, 2054, 2621, 2801, 2966
Esp3I	CGTCTC	1 cut(s)	9
FalI	AAGNNNNNCTT	4 cut(s)	858, 890, 2524, 2556
FauI	CCCGC	1 cut(s)	1329
FauNDI	CATATG	1 cut(s)	2551
FbaI	TGATCA	1 cut(s)	2017
FblI	GTMKAC	1 cut(s)	2669
Fnu4HI	GCNGC	5 cut(s)	5, 603, 1511, 2684, 2735
FriOI	GRGCYC	2 cut(s)	2032, 2465
Fsp4HI	GCNGC	5 cut(s)	5, 603, 1511, 2684, 2735
FspBI	CTAG	8 cut(s)	74, 1035, 2010, 2067, 2205, 2307, 2511, 2652
FspI	TGCGCA	1 cut(s)	740
GlaI	GCGC	3 cut(s)	226, 740, 2183
GluI	GCNGC	5 cut(s)	5, 603, 1511, 2684, 2735
GsaI	CCCAGC	3 cut(s)	1446, 2468, 2482
GsuI	CTGGAG	3 cut(s)	986, 2409, 2862
HaeII	RGCGCY	1 cut(s)	228
HaeIII	GGCC	3 cut(s)	72, 2076, 2582
HapII	CCGG	6 cut(s)	41, 276, 1204, 2254, 2726, 3018
HhaI	GCGC	3 cut(s)	227, 741, 2184
Hin1I	GRCGYC	1 cut(s)	225
Hin6I	GCGC	3 cut(s)	225, 739, 2182
HinP1I	GCGC	3 cut(s)	225, 739, 2182
HincII	GTYRAC	3 cut(s)	1248, 2251, 2670
HindII	GTYRAC	3 cut(s)	1248, 2251, 2670
HindIII	AAGCTT	1 cut(s)	1775
HpaII	CCGG	6 cut(s)	41, 276, 1204, 2254, 2726, 3018
HphI	GGTGA	7 cut(s)	580, 994, 1094, 1301, 1597, 2009, 2933
Hpy166II	GTNNAC	7 cut(s)	1064, 1248, 2064, 2210, 2251, 2516, 2670
Hpy8I	GTNNAC	7 cut(s)	1064, 1248, 2064, 2210, 2251, 2516, 2670
Hpy99I	CGWCG	9 cut(s)	170, 188, 209, 212, 215, 347, 350, 1838, 2671
HpyCH4III	ACNGT	9 cut(s)	919, 1363, 2143, 2177, 2248, 2520, 2630, 2929, 2935
HpyCH4IV	ACGT	7 cut(s)	168, 690, 1012, 1675, 1975, 2274, 2303
HpyF10VI	GCNNNNNNNGC	4 cut(s)	231, 1516, 1783, 2036
HpyF3I	CTNAG	6 cut(s)	1542, 1716, 1725, 1778, 2701, 3094
HpySE526I	ACGT	7 cut(s)	168, 690, 1012, 1675, 1975, 2274, 2303
Hsp92I	GRCGYC	1 cut(s)	225
HspAI	GCGC	3 cut(s)	225, 739, 2182
KasI	GGCGCC	1 cut(s)	224
Kpn2I	TCCGGA	1 cut(s)	1203
Ksp22I	TGATCA	1 cut(s)	2017
LmnI	GCTCC	8 cut(s)	37, 365, 1057, 2035, 2390, 2563, 2653, 2880
Lsp1109I	GCAGC	4 cut(s)	589, 1522, 2695, 2746
LweI	GCATC	6 cut(s)	390, 412, 1282, 2374, 2415, 2714
MaeI	CTAG	8 cut(s)	74, 1035, 2010, 2067, 2205, 2307, 2511, 2652
MaeII	ACGT	7 cut(s)	168, 690, 1012, 1675, 1975, 2274, 2303
MaeIII	GTNAC	8 cut(s)	8, 913, 1082, 1591, 1944, 1997, 2242, 2929
MfeI	CAATTG	1 cut(s)	816
MflI	RGATCY	3 cut(s)	445, 1069, 1420
MhlI	GDGCHC	5 cut(s)	1521, 2032, 2465, 2510, 2877
Mly113I	GGCGCC	1 cut(s)	225
MlyI	GAGTC	6 cut(s)	1896, 1994, 2234, 2365, 2413, 2665
MmeI	TCCRAC	2 cut(s)	399, 2585
Mph1103I	ATGCAT	1 cut(s)	405
MroI	TCCGGA	1 cut(s)	1203
MseI	TTAA	9 cut(s)	611, 926, 1116, 1704, 1740, 1745, 1797, 1941, 3081
MslI	CAYNNNNRTG	4 cut(s)	515, 1050, 1554, 2943
MspCI	CTTAAG	1 cut(s)	1703
MspI	CCGG	6 cut(s)	41, 276, 1204, 2254, 2726, 3018
MunI	CAATTG	1 cut(s)	816
Mva1269I	GAATGC	1 cut(s)	741
MvnI	CGCG	3 cut(s)	7, 234, 2184
MwoI	GCNNNNNNNGC	4 cut(s)	231, 1516, 1783, 2036
NarI	GGCGCC	1 cut(s)	225
NciI	CCSGG	1 cut(s)	2727
NcoI	CCATGG	1 cut(s)	2801
NdeI	CATATG	1 cut(s)	2551
NlaIV	GGNNCC	7 cut(s)	39, 226, 552, 872, 1864, 2161, 3013
NmeAIII	GCCGAG	1 cut(s)	526
NmuCI	GTSAC	5 cut(s)	8, 1082, 1591, 1944, 1997
NsbI	TGCGCA	1 cut(s)	740
NsiI	ATGCAT	1 cut(s)	405
NspI	RCATGY	3 cut(s)	1517, 1695, 1925
OliI	CACNNNNGTG	1 cut(s)	515
PaeI	GCATGC	1 cut(s)	1925
PaeR7I	CTCGAG	1 cut(s)	2222
PagI	TCATGA	2 cut(s)	1630, 2404
PceI	AGGCCT	1 cut(s)	72
PcsI	WCGNNNNNNNCGW	4 cut(s)	210, 315, 696, 1057
PctI	GAATGC	1 cut(s)	741
PfeI	GAWTC	6 cut(s)	311, 1447, 2041, 2149, 2335, 2443
PflMI	CCANNNNNTGG	2 cut(s)	2567, 2753
PfoI	TCCNGGA	3 cut(s)	2716, 2725, 2877
PkrI	GCNGC	5 cut(s)	6, 604, 1512, 2685, 2736
Ple19I	CGATCG	1 cut(s)	582
PleI	GAGTC	6 cut(s)	1895, 1994, 2233, 2365, 2413, 2665
PluTI	GGCGCC	1 cut(s)	228
PmaCI	CACGTG	1 cut(s)	1976
PmlI	CACGTG	1 cut(s)	1976
PpsI	GAGTC	6 cut(s)	1895, 1994, 2233, 2365, 2413, 2665
Ppu21I	YACGTR	2 cut(s)	1676, 1976
PpuMI	RGGWCCY	5 cut(s)	871, 1387, 1699, 1841, 1862
PshAI	GACNNNNGTC	1 cut(s)	127
PshBI	ATTAAT	1 cut(s)	926
Psp124BI	GAGCTC	1 cut(s)	2032
Psp5II	RGGWCCY	5 cut(s)	871, 1387, 1699, 1841, 1862
PspCI	CACGTG	1 cut(s)	1976
PspFI	CCCAGC	3 cut(s)	1442, 2464, 2478
PspN4I	GGNNCC	7 cut(s)	39, 226, 552, 872, 1864, 2161, 3013
PspPPI	RGGWCCY	5 cut(s)	871, 1387, 1699, 1841, 1862
PspXI	VCTCGAGB	1 cut(s)	2222
PsrI	GAACNNNNNNTAC	2 cut(s)	671, 703
PstNI	CAGNNNCTG	1 cut(s)	2753
PsuI	RGATCY	3 cut(s)	445, 1069, 1420
PvuI	CGATCG	1 cut(s)	582
RsaI	GTAC	9 cut(s)	903, 1162, 1433, 1471, 2211, 2632, 2660, 2832, 2937
RsaNI	GTAC	9 cut(s)	902, 1161, 1432, 1470, 2210, 2631, 2659, 2831, 2936
RseI	CAYNNNNRTG	4 cut(s)	515, 1050, 1554, 2943
SacI	GAGCTC	1 cut(s)	2032
SalI	GTCGAC	1 cut(s)	2668
SaqAI	TTAA	9 cut(s)	611, 926, 1116, 1704, 1740, 1745, 1797, 1941, 3081
SatI	GCNGC	5 cut(s)	5, 603, 1511, 2684, 2735
ScaI	AGTACT	3 cut(s)	1162, 1433, 2632
SchI	GAGTC	6 cut(s)	1896, 1994, 2234, 2365, 2413, 2665
SduI	GDGCHC	5 cut(s)	1521, 2032, 2465, 2510, 2877
SfaNI	GCATC	6 cut(s)	390, 412, 1282, 2374, 2415, 2714
SfcI	CTRYAG	2 cut(s)	2139, 2602
SfoI	GGCGCC	1 cut(s)	226
Sfr274I	CTCGAG	1 cut(s)	2222
SinI	GGWCC	8 cut(s)	129, 550, 871, 1387, 1699, 1841, 1862, 2798
SlaI	CTCGAG	1 cut(s)	2222
SmiMI	CAYNNNNRTG	4 cut(s)	515, 1050, 1554, 2943
SmlI	CTYRAG	3 cut(s)	1473, 1703, 2222
SmoI	CTYRAG	3 cut(s)	1473, 1703, 2222
SpeI	ACTAGT	2 cut(s)	2204, 2510
SphI	GCATGC	1 cut(s)	1925
SseBI	AGGCCT	1 cut(s)	72
SsiI	CCGC	9 cut(s)	5, 142, 173, 234, 240, 281, 1007, 1336, 2354
SspDI	GGCGCC	1 cut(s)	224
SspI	AATATT	2 cut(s)	1024, 3087
SspMI	CTAG	8 cut(s)	74, 1035, 2010, 2067, 2205, 2307, 2511, 2652
SstI	GAGCTC	1 cut(s)	2032
StuI	AGGCCT	1 cut(s)	72
StyI	CCWWGG	7 cut(s)	67, 879, 1848, 2054, 2621, 2801, 2966
TaaI	ACNGT	9 cut(s)	919, 1363, 2143, 2177, 2248, 2520, 2630, 2929, 2935
TaiI	ACGT	7 cut(s)	171, 693, 1015, 1678, 1978, 2277, 2306
TatI	WGTACW	4 cut(s)	1160, 1431, 2630, 2935
TauI	GCSGC	1 cut(s)	7
TfiI	GAWTC	6 cut(s)	311, 1447, 2041, 2149, 2335, 2443
Tru1I	TTAA	9 cut(s)	611, 926, 1116, 1704, 1740, 1745, 1797, 1941, 3081
Tru9I	TTAA	9 cut(s)	611, 926, 1116, 1704, 1740, 1745, 1797, 1941, 3081
TscAI	CASTG	1 cut(s)	517
TseFI	GTSAC	5 cut(s)	8, 1082, 1591, 1944, 1997
TseI	GCWGC	4 cut(s)	602, 1510, 2683, 2734
Tsp45I	GTSAC	5 cut(s)	8, 1082, 1591, 1944, 1997
TspGWI	ACGGA	5 cut(s)	139, 1544, 2436, 2560, 3059
TspRI	CASTG	1 cut(s)	517
Van91I	CCANNNNNTGG	2 cut(s)	2567, 2753
Vha464I	CTTAAG	1 cut(s)	1703
VpaK11BI	GGWCC	8 cut(s)	129, 550, 871, 1387, 1699, 1841, 1862, 2798
VspI	ATTAAT	1 cut(s)	926
XagI	CCTNNNNNAGG	4 cut(s)	77, 878, 2571, 2778
XapI	RAATTY	2 cut(s)	459, 822
XbaI	TCTAGA	1 cut(s)	2009
XceI	RCATGY	3 cut(s)	1517, 1695, 1925
XhoI	CTCGAG	1 cut(s)	2222
XmiI	GTMKAC	1 cut(s)	2669
XspI	CTAG	8 cut(s)	74, 1035, 2010, 2067, 2205, 2307, 2511, 2652
ZrmI	AGTACT	3 cut(s)	1162, 1433, 2632
Zsp2I	ATGCAT	1 cut(s)	405

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.