Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Forward (+)

32021469 .. 32030256

Gene Structure 8788 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00191440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3156 bp

ATGCCCCGTCACAGAGACGAAGACGACGACGAGATGGAGCCAGACGAGGACGAGTACGACCCGGACCAAGGTCTAGACGACGTCGACGACGAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGACCGAGGAGGGCGGAGGTCCAGTCAGAAGCGGAGGAGATCGAGCTTCATTGACGACGTGGCCGAGGAGGACGACGAGGAGGAAGAGGAGGACGACGAGGATGAGGATTACGGCGCCTACCGTGGCGGTGGCGGTGGGAGTCGTAAGAAGAGCAAAAAGAAGCCCTCCGGTGCGGATTTTCTTGATCTCGAAGCGGTGGTGGATTCGGATGAGGAGGAAGAGGAGGATGAGGGCGAGGACGACTTTATTGATTCTGGAGCTGATATACCTGAGGAGGGCGATGGTAGAAGGATGCATCATCGCCCATTGATGGATGACCAAGAGCAAGAAGATGTCGAGGCTCTGGAAAGAAGAATTCAGGCAAGATATGCAAAGCAACATCATTCGGAAGATACTGAGGAGACCACTGATGTGGATCAGCAAGCTCTTTTACCTTCTGTTTTGGACCCAAAGCTGTGGATGGTGAAATGTGCGATTGGTAAAGAACGAGAGGTAGCTGCTTGCTTAATGCAAAAGTATATTGATAAACCTGAATTGAACATCAGGTCTGTTATTGCTCTTGACCACCTGAAGAACTATATATACGTTGAAGCAGAAAAAGAAGCCCATGTGAGGGAGGCTTGCAAAGGATTGCGCAATATATTTGCCCAGAAAATAGCGCTTGTTCCTATTAGAGAAATGACTGATGTTCTTTCCGTTGAAAGCAAGGCAATTGATATTTCTAGGGATACTTGGGTCAGAATGAAGATTGGGATATATAAAGGGGACCTTGCCAAGGTTGTTGATGTGGATAATGTACGACAGAGAGTTACAGTAAAATTAATTCCAAGAATTGATTTACAAGCTCTTGCAAATAAACTGGAGGGCAGAGAAGTTGTGAAAAAGAAAGCATTTGTTCCTCCGCCACGTTTTATGAATATTGATGAAGCTAGAGAACTGCATATTCGTGTGGAGCGGAAACGAGATCCAATGACGGGTGACTACTTTGAAAACATTGAGAAAATGATGTTTAAAGATGGTTTCTTGTATAAAATTGTATCAATGAAATCAATAAGTACTCAAAACATACATCCCACTTTTGATGAACTGGAAAAGTTTCGAAAACCTGGGGAGAATGGTGAAGGAGATATAGCCAGTCTGTCAACTTTATTTTCAAACAGAAAGAAAGGACACTTTGTGAAGGGTGATGCAGTCATTGTCATCAAGGGAGACCTGAAAAACTTGAAAGGGCGGGTTGAGAAAGTTGAGGAAGGGACCGTGCATATCAGACCCGACATGAAAGACCTCCCAAAAACTCTTGCCATAAATGAAAAAGAACTTTGCAAGTACTTTGAACCTGGGAATCATGTGAAGGTTGTCTCTGGTACTCAAGAAGGTGCAACTGGTATGGTTGTCAAGGTCGATCAGCACGTGCTGATCATTTTATCTGATACAACTAAGGAACATCTCCGTGTGTTTTCTGATGATGTTGTGGAGAGTTCTGAGGTGACATCTGGTATTACCAAAATTGGGGACTATGAGCTTCATGATCTTGTGTTACTGGATAATAATAGCTTTGGTGTAATTATACGTGTTGAAACTGAAGCATGTCAGGTCCTTAAGGGAGTTCCTGAGAGACCTGAGGTTGCCCTTGTCAAATTAAGAGAGATCAAATGCAAGATTGAGAAAAAGCTTAGTGTGCAGGATTGTTTTAAGAACACAATATCTGTGAAAGATGTTGTGAGGGTCGTTGAAGGTCCTTCCAAGGGGAAACAAGGTCCTGTGGAACACATATACAGAGGAGTCTTATTCATTTATGATCGCCATCACATAGAACATGCGGGATTTATATGTGTTAAGTCACATTCTTGTCGTGTTGTAGGAGGATCACGTGCCAATGGTGATAGAAATGGTGACTCCTACTCTAGATTTGATCATCTTCGAGCTCCTCCTCTTATTCCCCAGTCTCCCAGGAGGTTTACTAGAGGAGGCCCTCCTAATAACTTTGGGGGAAGAAATAGAGGTGGAAGGGGGCATGATGGATTGGTTGGGACTACAGTAAAGATTCGCCAGGGTGCCTATAAGGGTTACCGTGGGCGCGTTGTTGAGGTTAAGGGAACTAGTGTACGGGTGGAACTTGAGTCCCAAATGAAAGTTGTGACAGTTGACCGGAATTGTATTTCTGATAACGTGGCTATTACAACACCGTATCGTGATACATCTAGCTATGGTATGGGAAGTCAGACCCCAATTCATCCTTCTCGAACTCCGCTCCATCCATATATGACTCCAATGAGGGATGCAGGAGCAACGCCTATTCATGATGGCATGAGGACTCCGATGCGTGACCGAGCCTGGAATCCGTATGCACCTATGAGTCCAGCAAGGGATAGCTGGGAGGATGGAAACCCTGGTTCTTGGGGTTCTAGCCCACAGTATCAGCCAGGAAGTCCTCCTTCACGGACGTATGAAGCTCCAACTCCTGGTTCAGGTTGGGCCAACACTCCTAGTGGAAATTATAGTGAAGCTGGAACACCGAGGGACAGTACTGGTTATGCAAATGCTCCTAGTCCATACTTGCCGTCGACTCCTGGTGGGCAGCCTATGACACCAAACTCAGCATCCTATCTTCCTGGAACTCCTGGAGGGCAGCCAATGACACCAGGGACTGGTGGTCTGGATATGATGTCTCCTGTTATAGGAGGGGACAATGAAGGACCGTGGTTCATGCCAGATATATTGGTCAATGTACGCAATTCTGGAGAGGAAACTACTGGAGTTGTCAGAGAAGTGCTCCTGGATGGCTCATGTAGGGTAGCTCTTGGCTCGGGTGATACTGTAACTGTACTTCCAAATGAAATGGAGGTAGTCGTACCAAGGAAGAATGATAAGATCAAAATCATGGGTGGCTCATTACGCGGTGCCACTGGCAAGCTGATTGGTGTCGATGGCACGGATGGAATTGTCAAGGTTGATGACACTCTTGATGTTAAAATATTAGACTTGGCTATTTTGTCAAAACTAGGCCAATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

1051

Amino Acids

116.93

Weight (kDa)

5.06

Isoelectric Point (pI)

45.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5_N	PF11942	107 - 192	3.4e-12	Spt5 transcription elongation factor, acidic N-terminal
Spt5-NGN	PF03439	198 - 278	1e-26	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	284 - 413	5.1e-76	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	441 - 490	4.2e-22	Transcription elongation factor SPT5, second KOW domain
KOWx_SPT5	PF23037	549 - 600	1.4e-17	Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5	PF23291	608 - 661	7e-24	Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5	PF23290	722 - 765	7.2e-18	Transcription elongation factor SPT5, fifth KOW domain
KOW6_SPT51-2	PF23038	949 - 995	2.8e-23	Transcription elongation factor SPT5 homolog 1/2, KOW6 domain
KOW7_SPT5	PF23287	997 - 1048	3.9e-21	Transcription elongation factor SPT5, seventh KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	84
Acc16I	TGCGCA	1 cut(s)	776
AccB1I	GGYRCC	3 cut(s)	254, 2195, 3041
AccB7I	CCANNNNNTGG	2 cut(s)	2603, 2789
AccBSI	CCGCTC	2 cut(s)	1096, 2392
AccI	GTMKAC	2 cut(s)	84, 2705
AccII	CGCG	2 cut(s)	2220, 3039
AclWI	GGATC	3 cut(s)	564, 1100, 2014
AcoI	YGGCCR	1 cut(s)	201
AcsI	RAATTY	1 cut(s)	495
AcuI	CTGAAG	2 cut(s)	731, 1743
AcvI	CACGTG	2 cut(s)	1552, 2012
AcyI	GRCGYC	2 cut(s)	81, 255
AdeI	CACNNNGTG	1 cut(s)	1318
AfeI	AGCGCT	1 cut(s)	801
AflII	CTTAAG	1 cut(s)	1739
AflIII	ACRYGT	1 cut(s)	1711
AgsI	TTSAA	9 cut(s)	679, 731, 842, 1130, 1296, 1366, 1475, 1718, 1874
AhdI	GACNNNNNGTC	1 cut(s)	2793
AhlI	ACTAGT	1 cut(s)	2240
AjiI	CACGTC	1 cut(s)	199
AjuI	GAANNNNNNNTTGG	4 cut(s)	2362, 2394, 2590, 2622
AleI	CACNNNNGTG	1 cut(s)	551
Alw21I	GWGCWC	2 cut(s)	2068, 2916
Alw26I	GTCTC	7 cut(s)	9, 536, 1344, 1504, 1750, 2091, 2814
AlwI	GGATC	3 cut(s)	564, 1100, 2014
AlwNI	CAGNNNCTG	1 cut(s)	2789
Ama87I	CYCGRG	1 cut(s)	2947
Aor51HI	AGCGCT	1 cut(s)	801
AoxI	GGCC	4 cut(s)	201, 2110, 2616, 3145
ApeKI	GCWGC	3 cut(s)	638, 2719, 2770
ApoI	RAATTY	1 cut(s)	495
AseI	ATTAAT	1 cut(s)	962
Asp700I	GAANNNNTTC	1 cut(s)	1236
AspLEI	GCGC	4 cut(s)	257, 777, 802, 2220
AsuC2I	CCSGG	1 cut(s)	62
AsuHPI	GGTGA	8 cut(s)	616, 1130, 1271, 1337, 1639, 2033, 2045, 2963
AsuII	TTCGAA	1 cut(s)	1240
AvaI	CYCGRG	1 cut(s)	2947
AxyI	CCTNAGG	2 cut(s)	411, 1761
BaeI	ACNNNNGTAYC	2 cut(s)	861, 894
BanI	GGYRCC	3 cut(s)	254, 2195, 3041
BanII	GRGCYC	1 cut(s)	2068
BarI	GAAGNNNNNNTAC	2 cut(s)	2952, 2984
BbrPI	CACGTG	2 cut(s)	1552, 2012
BbsI	GAAGAC	1 cut(s)	27
Bbv12I	GWGCWC	2 cut(s)	2068, 2916
BbvI	GCAGC	3 cut(s)	625, 2731, 2782
BceAI	ACGGC	2 cut(s)	268, 2686
BciVI	GTATCC	1 cut(s)	862
BclI	TGATCA	2 cut(s)	1557, 2053
BcnI	CCSGG	1 cut(s)	62
BcoDI	GTCTC	7 cut(s)	9, 536, 1344, 1504, 1750, 2091, 2814
BcuI	ACTAGT	1 cut(s)	2240
BfmI	CTRYAG	1 cut(s)	2175
BfoI	RGCGCY	2 cut(s)	258, 803
BfrI	CTTAAG	1 cut(s)	1739
BfuI	GTATCC	1 cut(s)	862
BisI	GCNGC	3 cut(s)	639, 2720, 2771
BlsI	GCNGC	3 cut(s)	640, 2721, 2772
BmcAI	AGTACT	3 cut(s)	1198, 1469, 2668
BmeRI	GACNNNNNGTC	1 cut(s)	2793
BmeT110I	CYCGRG	1 cut(s)	2947
BmgBI	CACGTC	1 cut(s)	199
BmiI	GGNNCC	7 cut(s)	39, 256, 588, 908, 1396, 2197, 3043
BmrI	ACTGGG	1 cut(s)	2077
BmsI	GCATC	6 cut(s)	423, 445, 1318, 2410, 2451, 2750
BmuI	ACTGGG	1 cut(s)	2077
BoxI	GACNNNNGTC	1 cut(s)	69
BpiI	GAAGAC	1 cut(s)	27
BplI	GAGNNNNNCTC	2 cut(s)	2898, 2930
BpmI	CTGGAG	5 cut(s)	417, 1022, 2784, 2901, 2916
Bpu14I	TTCGAA	1 cut(s)	1240
BpuEI	CTTGAG	2 cut(s)	1494, 2279
BpuMI	CCSGG	1 cut(s)	62
BsaAI	YACGTR	3 cut(s)	1552, 1712, 2012
BsaBI	GATNNNNATC	3 cut(s)	555, 1944, 3017
BsaHI	GRCGYC	2 cut(s)	81, 255
BsaI	GGTCTC	3 cut(s)	536, 1344, 1750
BsaWI	WCCGGW	2 cut(s)	308, 2289
BsaXI	ACNNNNNCTCC	2 cut(s)	148, 178
Bse21I	CCTNAGG	2 cut(s)	411, 1761
Bse8I	GATNNNNATC	3 cut(s)	555, 1944, 3017
BseJI	GATNNNNATC	3 cut(s)	555, 1944, 3017
BseMII	CTCAG	6 cut(s)	402, 528, 1614, 1743, 1752, 2751
BseXI	GCAGC	3 cut(s)	625, 2731, 2782
BseYI	CCCAGC	1 cut(s)	2514
BsgI	GTGCAG	1 cut(s)	1841
Bsh1236I	CGCG	2 cut(s)	2220, 3039
BshFI	GGCC	4 cut(s)	203, 2112, 2618, 3147
BshNI	GGYRCC	3 cut(s)	254, 2195, 3041
BsiHKAI	GWGCWC	2 cut(s)	2068, 2916
BsiHKCI	CYCGRG	1 cut(s)	2947
BsiSI	CCGG	3 cut(s)	62, 309, 2290
BslFI	GGGAC	8 cut(s)	920, 1408, 1667, 2185, 2248, 2675, 2800, 2840
BsmAI	GTCTC	7 cut(s)	9, 536, 1344, 1504, 1750, 2091, 2814
BsmBI	CGTCTC	1 cut(s)	9
BsmFI	GGGAC	8 cut(s)	920, 1408, 1667, 2185, 2248, 2675, 2800, 2840
BsnI	GGCC	4 cut(s)	203, 2112, 2618, 3147
Bso31I	GGTCTC	3 cut(s)	536, 1344, 1750
BsoBI	CYCGRG	1 cut(s)	2947
Bsp119I	TTCGAA	1 cut(s)	1240
Bsp1286I	GDGCHC	2 cut(s)	2068, 2916
BspANI	GGCC	4 cut(s)	203, 2112, 2618, 3147
BspCNI	CTCAG	6 cut(s)	403, 529, 1615, 1744, 1753, 2750
BspFNI	CGCG	2 cut(s)	2220, 3039
BspHI	TCATGA	2 cut(s)	1666, 2440
BspLI	GGNNCC	7 cut(s)	39, 256, 588, 908, 1396, 2197, 3043
BspPI	GGATC	3 cut(s)	564, 1100, 2014
BspQI	GCTCTTC	1 cut(s)	284
BspT104I	TTCGAA	1 cut(s)	1240
BspT107I	GGYRCC	3 cut(s)	254, 2195, 3041
BspTI	CTTAAG	1 cut(s)	1739
BspTNI	GGTCTC	3 cut(s)	536, 1344, 1750
BsrBI	CCGCTC	2 cut(s)	1096, 2392
BssNI	GRCGYC	2 cut(s)	81, 255
BssT1I	CCWWGG	4 cut(s)	67, 915, 1884, 2996
Bst6I	CTCTTC	4 cut(s)	87, 219, 284, 354
BstACI	GRCGYC	2 cut(s)	81, 255
BstAFI	CTTAAG	1 cut(s)	1739
BstAPI	GCANNNNNTGC	1 cut(s)	509
BstBAI	YACGTR	3 cut(s)	1552, 1712, 2012
BstBI	TTCGAA	1 cut(s)	1240
BstC8I	GCNNGC	4 cut(s)	564, 643, 763, 3053
BstDEI	CTNAG	8 cut(s)	411, 537, 1578, 1623, 1752, 1761, 1814, 2737
BstDSI	CCRYGG	3 cut(s)	262, 2212, 2840
BstEII	GGTNACC	1 cut(s)	2207
BstENI	CCTNNNNNAGG	3 cut(s)	914, 2607, 2817
BstFNI	CGCG	2 cut(s)	2220, 3039
BstH2I	RGCGCY	2 cut(s)	258, 803
BstHHI	GCGC	4 cut(s)	257, 777, 802, 2220
BstMAI	GTCTC	7 cut(s)	9, 536, 1344, 1504, 1750, 2091, 2814
BstMWI	GCNNNNNNNGC	3 cut(s)	509, 1819, 3036
BstNSI	RCATGY	2 cut(s)	1731, 1961
BstPAI	GACNNNNGTC	1 cut(s)	69
BstPI	GGTNACC	1 cut(s)	2207
BstSFI	CTRYAG	1 cut(s)	2175
BstUI	CGCG	2 cut(s)	2220, 3039
BstV1I	GCAGC	3 cut(s)	625, 2731, 2782
BstV2I	GAAGAC	1 cut(s)	27
BstX2I	RGATCY	1 cut(s)	1105
BstXI	CCANNNNNNTGG	2 cut(s)	553, 597
BstYI	RGATCY	1 cut(s)	1105
Bsu36I	CCTNAGG	2 cut(s)	411, 1761
BsuI	GTATCC	1 cut(s)	862
BsuRI	GGCC	4 cut(s)	203, 2112, 2618, 3147
BtgI	CCRYGG	3 cut(s)	262, 2212, 2840
BtgZI	GCGATG	2 cut(s)	425, 435
BtrI	CACGTC	1 cut(s)	199
BtsIMutI	CAGTG	2 cut(s)	546, 3045
Cac8I	GCNNGC	4 cut(s)	564, 643, 763, 3053
CaiI	CAGNNNCTG	1 cut(s)	2789
CciI	TCATGA	2 cut(s)	1666, 2440
CfoI	GCGC	4 cut(s)	257, 777, 802, 2220
DdeI	CTNAG	8 cut(s)	411, 537, 1578, 1623, 1752, 1761, 1814, 2737
DinI	GGCGCC	1 cut(s)	256
DraI	TTTAAA	1 cut(s)	1153
DraIII	CACNNNGTG	1 cut(s)	1318
DriI	GACNNNNNGTC	1 cut(s)	2793
EaeI	YGGCCR	1 cut(s)	201
Eam1104I	CTCTTC	4 cut(s)	87, 219, 284, 354
Eam1105I	GACNNNNNGTC	1 cut(s)	2793
EarI	CTCTTC	4 cut(s)	87, 219, 284, 354
EciI	GGCGGA	2 cut(s)	169, 1032
Ecl136II	GAGCTC	1 cut(s)	2066
Eco130I	CCWWGG	4 cut(s)	67, 915, 1884, 2996
Eco24I	GRGCYC	1 cut(s)	2068
Eco31I	GGTCTC	3 cut(s)	536, 1344, 1750
Eco47III	AGCGCT	1 cut(s)	801
Eco53kI	GAGCTC	1 cut(s)	2066
Eco57I	CTGAAG	2 cut(s)	731, 1743
Eco72I	CACGTG	2 cut(s)	1552, 2012
Eco81I	CCTNAGG	2 cut(s)	411, 1761
Eco88I	CYCGRG	1 cut(s)	2947
Eco91I	GGTNACC	1 cut(s)	2207
EcoICRI	GAGCTC	1 cut(s)	2066
EcoNI	CCTNNNNNAGG	3 cut(s)	914, 2607, 2817
EcoO109I	RGGNCCY	5 cut(s)	907, 1735, 1877, 1898, 2111
EcoO65I	GGTNACC	1 cut(s)	2207
EcoRI	GAATTC	1 cut(s)	495
EcoT14I	CCWWGG	4 cut(s)	67, 915, 1884, 2996
EcoT22I	ATGCAT	1 cut(s)	438
EcoT38I	GRGCYC	1 cut(s)	2068
EgeI	GGCGCC	1 cut(s)	256
EheI	GGCGCC	1 cut(s)	256
ErhI	CCWWGG	4 cut(s)	67, 915, 1884, 2996
Esp3I	CGTCTC	1 cut(s)	9
FalI	AAGNNNNNCTT	4 cut(s)	894, 926, 2560, 2592
FaqI	GGGAC	8 cut(s)	920, 1408, 1667, 2185, 2248, 2675, 2800, 2840
FauI	CCCGC	2 cut(s)	1365, 1954
FbaI	TGATCA	2 cut(s)	1557, 2053
FblI	GTMKAC	2 cut(s)	84, 2705
Fnu4HI	GCNGC	3 cut(s)	639, 2720, 2771
FriOI	GRGCYC	1 cut(s)	2068
Fsp4HI	GCNGC	3 cut(s)	639, 2720, 2771
FspI	TGCGCA	1 cut(s)	776
GlaI	GCGC	4 cut(s)	256, 776, 801, 2219
GluI	GCNGC	3 cut(s)	639, 2720, 2771
GsaI	CCCAGC	1 cut(s)	2518
GsuI	CTGGAG	5 cut(s)	417, 1022, 2784, 2901, 2916
HaeII	RGCGCY	2 cut(s)	258, 803
HaeIII	GGCC	4 cut(s)	203, 2112, 2618, 3147
HapII	CCGG	3 cut(s)	62, 309, 2290
HhaI	GCGC	4 cut(s)	257, 777, 802, 2220
Hin1I	GRCGYC	2 cut(s)	81, 255
Hin6I	GCGC	4 cut(s)	255, 775, 800, 2218
HinP1I	GCGC	4 cut(s)	255, 775, 800, 2218
HincII	GTYRAC	4 cut(s)	85, 1284, 2287, 2706
HindII	GTYRAC	4 cut(s)	85, 1284, 2287, 2706
HindIII	AAGCTT	1 cut(s)	1811
HpaII	CCGG	3 cut(s)	62, 309, 2290
HphI	GGTGA	8 cut(s)	616, 1130, 1271, 1337, 1639, 2033, 2045, 2963
Hpy166II	GTNNAC	6 cut(s)	85, 1284, 2100, 2246, 2287, 2706
Hpy8I	GTNNAC	6 cut(s)	85, 1284, 2100, 2246, 2287, 2706
HpyCH4IV	ACGT	9 cut(s)	81, 198, 726, 1048, 1551, 1711, 2011, 2310, 2585
HpyF10VI	GCNNNNNNNGC	3 cut(s)	509, 1819, 3036
HpyF3I	CTNAG	8 cut(s)	411, 537, 1578, 1623, 1752, 1761, 1814, 2737
HpySE526I	ACGT	9 cut(s)	81, 198, 726, 1048, 1551, 1711, 2011, 2310, 2585
Hsp92I	GRCGYC	2 cut(s)	81, 255
HspAI	GCGC	4 cut(s)	255, 775, 800, 2218
KasI	GGCGCC	1 cut(s)	254
Ksp22I	TGATCA	2 cut(s)	1557, 2053
LguI	GCTCTTC	1 cut(s)	284
LmnI	GCTCC	9 cut(s)	37, 398, 1093, 2071, 2397, 2426, 2599, 2689, 2919
Lsp1109I	GCAGC	3 cut(s)	625, 2731, 2782
LweI	GCATC	6 cut(s)	423, 445, 1318, 2410, 2451, 2750
MaeII	ACGT	9 cut(s)	81, 198, 726, 1048, 1551, 1711, 2011, 2310, 2585
MbiI	CCGCTC	2 cut(s)	1096, 2392
MfeI	CAATTG	1 cut(s)	852
MflI	RGATCY	1 cut(s)	1105
MhlI	GDGCHC	2 cut(s)	2068, 2916
Mly113I	GGCGCC	1 cut(s)	255
MlyI	GAGTC	8 cut(s)	289, 1932, 2030, 2270, 2401, 2449, 2506, 2701
MmeI	TCCRAC	1 cut(s)	2621
Mph1103I	ATGCAT	1 cut(s)	438
MroXI	GAANNNNTTC	1 cut(s)	1236
MseI	TTAA	9 cut(s)	647, 962, 1152, 1740, 1781, 1833, 1977, 2232, 3111
MslI	CAYNNNNRTG	3 cut(s)	551, 1086, 1590
MspCI	CTTAAG	1 cut(s)	1739
MspI	CCGG	3 cut(s)	62, 309, 2290
MunI	CAATTG	1 cut(s)	852
MvnI	CGCG	2 cut(s)	2220, 3039
MwoI	GCNNNNNNNGC	3 cut(s)	509, 1819, 3036
NarI	GGCGCC	1 cut(s)	255
NciI	CCSGG	1 cut(s)	62
NlaIV	GGNNCC	7 cut(s)	39, 256, 588, 908, 1396, 2197, 3043
NmeAIII	GCCGAG	1 cut(s)	229
NmuCI	GTSAC	7 cut(s)	8, 1118, 1627, 1980, 2033, 2278, 2465
NsbI	TGCGCA	1 cut(s)	776
NsiI	ATGCAT	1 cut(s)	438
NspI	RCATGY	2 cut(s)	1731, 1961
NspV	TTCGAA	1 cut(s)	1240
OliI	CACNNNNGTG	1 cut(s)	551
PagI	TCATGA	2 cut(s)	1666, 2440
PciSI	GCTCTTC	1 cut(s)	284
PcsI	WCGNNNNNNNCGW	5 cut(s)	24, 84, 87, 201, 1548
PdmI	GAANNNNTTC	1 cut(s)	1236
PfeI	GAWTC	5 cut(s)	344, 392, 1483, 2185, 2479
PflFI	GACNNNGTC	1 cut(s)	80
PflMI	CCANNNNNTGG	2 cut(s)	2603, 2789
PfoI	TCCNGGA	3 cut(s)	2752, 2761, 2916
PkrI	GCNGC	3 cut(s)	640, 2721, 2772
PleI	GAGTC	8 cut(s)	288, 1931, 2030, 2269, 2401, 2449, 2505, 2701
PluTI	GGCGCC	1 cut(s)	258
PmaCI	CACGTG	2 cut(s)	1552, 2012
PmlI	CACGTG	2 cut(s)	1552, 2012
PpsI	GAGTC	8 cut(s)	288, 1931, 2030, 2269, 2401, 2449, 2505, 2701
Ppu21I	YACGTR	3 cut(s)	1552, 1712, 2012
PpuMI	RGGWCCY	4 cut(s)	907, 1735, 1877, 1898
PshAI	GACNNNNGTC	1 cut(s)	69
PshBI	ATTAAT	1 cut(s)	962
Psp124BI	GAGCTC	1 cut(s)	2068
Psp5II	RGGWCCY	4 cut(s)	907, 1735, 1877, 1898
PspCI	CACGTG	2 cut(s)	1552, 2012
PspEI	GGTNACC	1 cut(s)	2207
PspFI	CCCAGC	1 cut(s)	2514
PspN4I	GGNNCC	7 cut(s)	39, 256, 588, 908, 1396, 2197, 3043
PspPPI	RGGWCCY	4 cut(s)	907, 1735, 1877, 1898
PsrI	GAACNNNNNNTAC	2 cut(s)	707, 739
PstNI	CAGNNNCTG	1 cut(s)	2789
PsuI	RGATCY	1 cut(s)	1105
PsyI	GACNNNGTC	1 cut(s)	80
RseI	CAYNNNNRTG	3 cut(s)	551, 1086, 1590
SacI	GAGCTC	1 cut(s)	2068
SalI	GTCGAC	2 cut(s)	83, 2704
SapI	GCTCTTC	1 cut(s)	284
SaqAI	TTAA	9 cut(s)	647, 962, 1152, 1740, 1781, 1833, 1977, 2232, 3111
SatI	GCNGC	3 cut(s)	639, 2720, 2771
ScaI	AGTACT	3 cut(s)	1198, 1469, 2668
SchI	GAGTC	8 cut(s)	289, 1932, 2030, 2270, 2401, 2449, 2506, 2701
SduI	GDGCHC	2 cut(s)	2068, 2916
SfaNI	GCATC	6 cut(s)	423, 445, 1318, 2410, 2451, 2750
SfcI	CTRYAG	1 cut(s)	2175
SfoI	GGCGCC	1 cut(s)	256
SfuI	TTCGAA	1 cut(s)	1240
SgrDI	CGTCGACG	1 cut(s)	83
SmiMI	CAYNNNNRTG	3 cut(s)	551, 1086, 1590
SmlI	CTYRAG	3 cut(s)	1509, 1739, 2258
SmoI	CTYRAG	3 cut(s)	1509, 1739, 2258
SpeI	ACTAGT	1 cut(s)	2240
SspDI	GGCGCC	1 cut(s)	254
SspI	AATATT	2 cut(s)	1060, 3117
SstI	GAGCTC	1 cut(s)	2068
StyI	CCWWGG	4 cut(s)	67, 915, 1884, 2996
TaiI	ACGT	9 cut(s)	84, 201, 729, 1051, 1554, 1714, 2014, 2313, 2588
TaqII	GACCGA	2 cut(s)	159, 2484
TatI	WGTACW	4 cut(s)	1196, 1467, 2666, 2965
TfiI	GAWTC	5 cut(s)	344, 392, 1483, 2185, 2479
Tru1I	TTAA	9 cut(s)	647, 962, 1152, 1740, 1781, 1833, 1977, 2232, 3111
Tru9I	TTAA	9 cut(s)	647, 962, 1152, 1740, 1781, 1833, 1977, 2232, 3111
TscAI	CASTG	2 cut(s)	553, 3052
TseFI	GTSAC	7 cut(s)	8, 1118, 1627, 1980, 2033, 2278, 2465
TseI	GCWGC	3 cut(s)	638, 2719, 2770
Tsp45I	GTSAC	7 cut(s)	8, 1118, 1627, 1980, 2033, 2278, 2465
TspGWI	ACGGA	5 cut(s)	826, 1580, 2472, 2596, 3089
TspRI	CASTG	2 cut(s)	553, 3052
Tth111I	GACNNNGTC	1 cut(s)	80
Van91I	CCANNNNNTGG	2 cut(s)	2603, 2789
Vha464I	CTTAAG	1 cut(s)	1739
VspI	ATTAAT	1 cut(s)	962
XagI	CCTNNNNNAGG	3 cut(s)	914, 2607, 2817
XapI	RAATTY	1 cut(s)	495
XbaI	TCTAGA	2 cut(s)	73, 2045
XceI	RCATGY	2 cut(s)	1731, 1961
XmiI	GTMKAC	2 cut(s)	84, 2705
XmnI	GAANNNNTTC	1 cut(s)	1236
ZraI	GACGTC	1 cut(s)	82
ZrmI	AGTACT	3 cut(s)	1198, 1469, 2668
Zsp2I	ATGCAT	1 cut(s)	438

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.