Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Reverse (-)

51207259 .. 51209635

Gene Structure 2377 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G024810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2073 bp

ATGCGAACCGGTTCGGCTTGGCTCGAACCGATTCGTAATCGTGGTCCTCCTCAATCTCCTTCTTCTCGCGGTTTTTCTCATTCTTATCCTTGGATTGGGCTTGGCGGCGTGATGCTTGCAGCCATGCCAAATCGGAGAGAGGATTATGAAGAAGAAGAGGAGAGCGAGATGCGGGAGGAGCGAAGGGCCAATTCGAAGCGGGGGAGATTGAATTCTGATGAGATTGAGATCCAGAAGCGGAGAGGGCGAGGGTCGAATTCGAAAAAGAGGAAATTGAAATCGGTTGAGGAGGAGGAAGAAGAAGAGAAGAAGGCAGTGTCGGTGTTTGTGGATGATGAGGCTATAGAGGATGAAGATGAAGATGAGGAGGAAGACGATGATGGTGAATTGGATGGGTTTATTGTTAATGAACCGGAGTTTGATAACGAACCTGATGAAGTTGAACATGATGGTGGCGGTGGGAGAAGGATGGGTTTTGTGTTGCCGGAGGAAGAAGAGGAGGAGGATATTGATGAGTTTGAAAGAAGGGTTCAAGACAGATTTAGGGATGTTCATGTCGAAGAATATGAGGATGATACGGAGGTTGGACAGCAAGCTCTTATACCCGGTGTGAAGGACCCGAAATTGTGGTTGGTCAAGTGTGCCATTGGTAGAGAGAGAGAGGTAGCGGCTTGTCTAATGCAGAAGTGCATTGATAATCCTGGAGAATTGCAGATCATGTCTGCAATTGCTTTGGATCACTTGAAAAGCTTCATATATGTTGAAGCTTACAAAGAAGCCCATGTGAGGGAAGCTTGCAGAGGTCTGAGGTATGTAATTCTGCAGAAAATAATGCTTGTTCCTATTAAAGAGATGATTGATGTGCTATCATGTGAAAGCAAGAGCGTTGTAAATGATAAATGGGTTAGGATTAAGACGGGAGTATATAAAGGGGACCTTGCCAAGATTGTGGGGCATGTCCCGGGGAGACAGCAAGTTAGAGTAAAGTTAATTCCAAGGGTTGATTTACAAGCTCTTGCAAAAGGTGTAGAATCATCGAAGAAGAAGGAATCTGGTGCACGTCCTGATCGTGTTCCTGCACTTCTTAGGAACATGAACCTAGTTAGACAGCTGGGTGTACGAGTACAGTCCATGCGAGATCCATTGGCACGTGGTTATACTGAGAAGATTGATGGCTTATCTGGGATGATCTTTAAAGATGGTTTCCATCTTAAAGTTGTTTCTGATAAAAGTATAAAAGAGGCAAAACCCTCCTTGCAAGAACTTGAAGAATTCCAGGAACTTGATGGTGATGGAGATCATACAATTCCAAATAGAAAGAAAGGCGAAAGCTTTATGAAGGGCGATGCAGTTATTGTTACGGAGGGAGATCTGAGAAACTTGGTGGGACGGGTTGAGAAAGTGGAGTTAGGGACTGTTCATATCAAAGCGGAAATGGATGGCCTCCCGGAAACTGTTGCCATCAATCAAACAGCACTTCAGAAATGCTTTAAAGAAGGGCAGCATGTGAAGGTTGTTAGTGGTAGTCAACAAGGAGCAACAGGCATGGTTGTTAAGGTTGATCAGCAGAAGCAGTTGATCACCATTTTATCTGATGCAGCCAATGAAGCTATCTGCGTGTTCTCTGATCATGTTGTGGAGAGTCCTGAAGTAAATAATGGTGATACAATGTGTGACTCCCACAACTTAAGGTATCACAAAACCCCTGGTCGAAGTCCGAGGTTTTCTGGAGGTGCAAGAAGGCATGATGGGCAGGATGGCTTTGAATCAGGTAATGTTGCTGCTGCAGTTACAACACCATATCGCTCTGGTTGTGAGACCCCAATGCCTTACATGAGGACTCCTATGAGAGATCCTGGAGCAACGTCAATGCCTTACATGACAACTCCCATGAGAGATCCTGGAGCATCCCCAATGCGTTATATGATGACTCCTTACATGATGACCCCCAGAAGAGATCCCCCCCGAGCAACACCAATACATGGTGGCATGAGAACTCCACCTATGACTCCCATGAGAGATCCCCGAGCAACACCGATACATGGTGGCATGAGGACTCCTCCTATATATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

690

Amino Acids

77.57

Weight (kDa)

5.44

Isoelectric Point (pI)

51.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5-NGN	PF03439	208 - 289	4.2e-24	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	297 - 425	6.1e-27	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	447 - 496	5e-16	Transcription elongation factor SPT5, second KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1982
AccII	CGCG	1 cut(s)	69
AciI	CCGC	8 cut(s)	69, 105, 172, 199, 238, 456, 668, 1430
AclWI	GGATC	7 cut(s)	223, 744, 1133, 1847, 1892, 1952, 2015
AcsI	RAATTY	3 cut(s)	211, 256, 1271
AcuI	CTGAAG	2 cut(s)	1463, 1668
AcvI	CACGTG	1 cut(s)	1151
AfaI	GTAC	2 cut(s)	1119, 1125
AfiI	CCNNNNNNNGG	5 cut(s)	95, 786, 787, 1982, 2042
AflII	CTTAAG	1 cut(s)	1687
AgeI	ACCGGT	1 cut(s)	8
AgsI	TTSAA	9 cut(s)	211, 277, 443, 521, 533, 745, 764, 1268, 1766
AjiI	CACGTC	1 cut(s)	1061
AjnI	CCWGG	5 cut(s)	700, 1275, 1705, 1855, 1900
AjuI	GAANNNNNNNTTGG	2 cut(s)	614, 646
AluBI	AGCT	8 cut(s)	596, 750, 767, 794, 1013, 1111, 1332, 1610
AluI	AGCT	8 cut(s)	596, 750, 767, 794, 1013, 1111, 1332, 1610
Alw21I	GWGCWC	1 cut(s)	1060
Alw26I	GTCTC	2 cut(s)	961, 1811
Alw44I	GTGCAC	1 cut(s)	1056
AlwI	GGATC	7 cut(s)	223, 744, 1133, 1847, 1892, 1952, 2015
Ama87I	CYCGRG	3 cut(s)	961, 1965, 2025
AoxI	GGCC	2 cut(s)	186, 1441
ApaLI	GTGCAC	1 cut(s)	1056
ApeKI	GCWGC	5 cut(s)	119, 1501, 1598, 1781, 1784
ApoI	RAATTY	3 cut(s)	211, 256, 1271
AsiGI	ACCGGT	1 cut(s)	8
Asp700I	GAANNNNTTC	3 cut(s)	10, 30, 749
AspS9I	GGNCC	4 cut(s)	44, 186, 616, 934
AsuC2I	CCSGG	4 cut(s)	606, 962, 963, 1448
AsuHPI	GGTGA	4 cut(s)	395, 1301, 1573, 1673
AsuII	TTCGAA	2 cut(s)	194, 260
AvaI	CYCGRG	3 cut(s)	961, 1965, 2025
AvaII	GGWCC	3 cut(s)	44, 616, 934
BaeGI	GKGCMC	1 cut(s)	1060
BaeI	ACNNNNGTAYC	2 cut(s)	567, 600
BbrPI	CACGTG	1 cut(s)	1151
BbsI	GAAGAC	1 cut(s)	378
Bbv12I	GWGCWC	1 cut(s)	1060
BbvI	GCAGC	5 cut(s)	131, 1513, 1610, 1768, 1771
BciT130I	CCWGG	5 cut(s)	702, 1277, 1707, 1857, 1902
BclI	TGATCA	3 cut(s)	1561, 1578, 1627
BcnI	CCSGG	4 cut(s)	606, 962, 963, 1448
BcoDI	GTCTC	2 cut(s)	961, 1811
BfaI	CTAG	1 cut(s)	1100
BfmI	CTRYAG	3 cut(s)	342, 821, 1785
BfrI	CTTAAG	1 cut(s)	1687
BglII	AGATCT	1 cut(s)	1369
BisI	GCNGC	7 cut(s)	106, 120, 669, 1502, 1599, 1782, 1785
BlsI	GCNGC	7 cut(s)	107, 121, 670, 1503, 1600, 1783, 1786
Bme1390I	CCNGG	9 cut(s)	606, 702, 962, 963, 1277, 1448, 1707, 1857, 1902
Bme18I	GGWCC	3 cut(s)	44, 616, 934
BmeT110I	CYCGRG	3 cut(s)	961, 1965, 2025
BmgBI	CACGTC	1 cut(s)	1061
BmgT120I	GGNCC	4 cut(s)	44, 186, 616, 934
BmiI	GGNNCC	2 cut(s)	618, 935
BmrFI	CCNGG	9 cut(s)	606, 702, 962, 963, 1277, 1448, 1707, 1857, 1902
BmsI	GCATC	5 cut(s)	102, 159, 1336, 1585, 1916
BpiI	GAAGAC	1 cut(s)	378
BplI	GAGNNNNNCTC	1 cut(s)	2044
BpmI	CTGGAG	4 cut(s)	723, 1749, 1878, 1923
Bpu14I	TTCGAA	2 cut(s)	194, 260
BpuMI	CCSGG	4 cut(s)	606, 962, 963, 1448
BsaAI	YACGTR	1 cut(s)	1151
BsaBI	GATNNNNATC	2 cut(s)	227, 1296
BsaI	GGTCTC	1 cut(s)	1811
BsaJI	CCNNGG	6 cut(s)	89, 961, 962, 995, 1705, 1718
BsaWI	WCCGGW	2 cut(s)	8, 412
Bsc4I	CCNNNNNNNGG	5 cut(s)	95, 786, 787, 1982, 2042
Bse118I	RCCGGY	1 cut(s)	8
Bse8I	GATNNNNATC	2 cut(s)	227, 1296
BseBI	CCWGG	5 cut(s)	702, 1277, 1707, 1857, 1902
BseDI	CCNNGG	6 cut(s)	89, 961, 962, 995, 1705, 1718
BseJI	GATNNNNATC	2 cut(s)	227, 1296
BseLI	CCNNNNNNNGG	5 cut(s)	95, 786, 787, 1982, 2042
BseMII	CTCAG	3 cut(s)	797, 1152, 1364
BseRI	GAGGAG	9 cut(s)	39, 173, 191, 302, 305, 380, 512, 515, 2049
BseSI	GKGCMC	1 cut(s)	1060
BseXI	GCAGC	5 cut(s)	131, 1513, 1610, 1768, 1771
BseYI	CCCAGC	1 cut(s)	1111
BsgI	GTGCAG	1 cut(s)	1062
Bsh1236I	CGCG	1 cut(s)	69
BshFI	GGCC	2 cut(s)	188, 1443
BshTI	ACCGGT	1 cut(s)	8
BsiHKAI	GWGCWC	1 cut(s)	1060
BsiHKCI	CYCGRG	3 cut(s)	961, 1965, 2025
BsiSI	CCGG	6 cut(s)	9, 413, 485, 606, 962, 1448
BslFI	GGGAC	4 cut(s)	944, 947, 1401, 1426
BslI	CCNNNNNNNGG	5 cut(s)	95, 786, 787, 1982, 2042
BsmAI	GTCTC	2 cut(s)	961, 1811
BsmFI	GGGAC	4 cut(s)	944, 947, 1401, 1426
BsnI	GGCC	2 cut(s)	188, 1443
Bso31I	GGTCTC	1 cut(s)	1811
BsoBI	CYCGRG	3 cut(s)	961, 1965, 2025
Bsp119I	TTCGAA	2 cut(s)	194, 260
Bsp1286I	GDGCHC	1 cut(s)	1060
BspACI	CCGC	8 cut(s)	69, 105, 172, 199, 238, 456, 668, 1430
BspANI	GGCC	2 cut(s)	188, 1443
BspCNI	CTCAG	3 cut(s)	798, 1153, 1365
BspFNI	CGCG	1 cut(s)	69
BspLI	GGNNCC	2 cut(s)	618, 935
BspMAI	CTGCAG	2 cut(s)	825, 1789
BspPI	GGATC	7 cut(s)	223, 744, 1133, 1847, 1892, 1952, 2015
BspT104I	TTCGAA	2 cut(s)	194, 260
BspTI	CTTAAG	1 cut(s)	1687
BspTNI	GGTCTC	1 cut(s)	1811
BsrFI	RCCGGY	1 cut(s)	8
BssAI	RCCGGY	1 cut(s)	8
BssECI	CCNNGG	6 cut(s)	89, 961, 962, 995, 1705, 1718
BssT1I	CCWWGG	2 cut(s)	89, 995
Bst2UI	CCWGG	5 cut(s)	702, 1277, 1707, 1857, 1902
Bst4CI	ACNGT	3 cut(s)	1128, 1417, 1456
Bst6I	CTCTTC	4 cut(s)	150, 297, 489, 1948
BstAFI	CTTAAG	1 cut(s)	1687
BstBAI	YACGTR	1 cut(s)	1151
BstBI	TTCGAA	2 cut(s)	194, 260
BstC8I	GCNNGC	3 cut(s)	117, 594, 796
BstDEI	CTNAG	4 cut(s)	806, 1085, 1161, 1373
BstFNI	CGCG	1 cut(s)	69
BstMAI	GTCTC	2 cut(s)	961, 1811
BstMWI	GCNNNNNNNGC	4 cut(s)	178, 244, 1607, 1750
BstNI	CCWGG	5 cut(s)	702, 1277, 1707, 1857, 1902
BstNSI	RCATGY	2 cut(s)	959, 1508
BstSCI	CCNGG	9 cut(s)	604, 700, 960, 961, 1275, 1446, 1705, 1855, 1900
BstSFI	CTRYAG	3 cut(s)	342, 821, 1785
BstSLI	GKGCMC	1 cut(s)	1060
BstUI	CGCG	1 cut(s)	69
BstV1I	GCAGC	5 cut(s)	131, 1513, 1610, 1768, 1771
BstV2I	GAAGAC	1 cut(s)	378
BstX2I	RGATCY	7 cut(s)	228, 1138, 1369, 1852, 1897, 1957, 2020
BstXI	CCANNNNNNTGG	1 cut(s)	949
BstYI	RGATCY	7 cut(s)	228, 1138, 1369, 1852, 1897, 1957, 2020
BsuRI	GGCC	2 cut(s)	188, 1443
BtgZI	GCGATG	1 cut(s)	1359
BtrI	CACGTC	1 cut(s)	1061
BtsI	GCAGTG	1 cut(s)	321
BtsIMutI	CAGTG	1 cut(s)	321
Cac8I	GCNNGC	3 cut(s)	117, 594, 796
Cfr10I	RCCGGY	1 cut(s)	8
Cfr13I	GGNCC	4 cut(s)	44, 186, 616, 934
Cfr9I	CCCGGG	1 cut(s)	961
Csp6I	GTAC	2 cut(s)	1118, 1124
CspAI	ACCGGT	1 cut(s)	8
CviQI	GTAC	2 cut(s)	1118, 1124
DdeI	CTNAG	4 cut(s)	806, 1085, 1161, 1373
DraI	TTTAAA	2 cut(s)	1195, 1492
Eam1104I	CTCTTC	4 cut(s)	150, 297, 489, 1948
EarI	CTCTTC	4 cut(s)	150, 297, 489, 1948
Eco130I	CCWWGG	2 cut(s)	89, 995
Eco31I	GGTCTC	1 cut(s)	1811
Eco47I	GGWCC	3 cut(s)	44, 616, 934
Eco57I	CTGAAG	2 cut(s)	1463, 1668
Eco72I	CACGTG	1 cut(s)	1151
Eco88I	CYCGRG	3 cut(s)	961, 1965, 2025
EcoO109I	RGGNCCY	2 cut(s)	616, 934
EcoRI	GAATTC	3 cut(s)	211, 256, 1271
EcoRII	CCWGG	5 cut(s)	700, 1275, 1705, 1855, 1900
EcoT14I	CCWWGG	2 cut(s)	89, 995
ErhI	CCWWGG	2 cut(s)	89, 995
FalI	AAGNNNNNCTT	2 cut(s)	921, 953
FaqI	GGGAC	4 cut(s)	944, 947, 1401, 1426
FauI	CCCGC	2 cut(s)	165, 192
FbaI	TGATCA	3 cut(s)	1561, 1578, 1627
Fnu4HI	GCNGC	7 cut(s)	106, 120, 669, 1502, 1599, 1782, 1785
Fsp4HI	GCNGC	7 cut(s)	106, 120, 669, 1502, 1599, 1782, 1785
FspBI	CTAG	1 cut(s)	1100
GluI	GCNGC	7 cut(s)	106, 120, 669, 1502, 1599, 1782, 1785
GsaI	CCCAGC	1 cut(s)	1115
GsuI	CTGGAG	4 cut(s)	723, 1749, 1878, 1923
HaeIII	GGCC	2 cut(s)	188, 1443
HapII	CCGG	6 cut(s)	9, 413, 485, 606, 962, 1448
HincII	GTYRAC	1 cut(s)	1529
HindII	GTYRAC	1 cut(s)	1529
HindIII	AAGCTT	4 cut(s)	748, 765, 792, 1330
HpaII	CCGG	6 cut(s)	9, 413, 485, 606, 962, 1448
HphI	GGTGA	4 cut(s)	395, 1301, 1573, 1673
Hpy166II	GTNNAC	3 cut(s)	1058, 1118, 1529
Hpy188I	TCNGA	9 cut(s)	135, 217, 807, 1225, 1374, 1482, 1594, 1627, 1719
Hpy188III	TCNNGA	5 cut(s)	232, 533, 1064, 1646, 1728
Hpy8I	GTNNAC	3 cut(s)	1058, 1118, 1529
HpyCH4III	ACNGT	3 cut(s)	1128, 1417, 1456
HpyCH4IV	ACGT	3 cut(s)	1060, 1150, 1865
HpyF10VI	GCNNNNNNNGC	4 cut(s)	178, 244, 1607, 1750
HpyF3I	CTNAG	4 cut(s)	806, 1085, 1161, 1373
HpySE526I	ACGT	3 cut(s)	1060, 1150, 1865
Ksp22I	TGATCA	3 cut(s)	1561, 1578, 1627
LmnI	GCTCC	4 cut(s)	178, 1535, 1859, 1904
Lsp1109I	GCAGC	5 cut(s)	131, 1513, 1610, 1768, 1771
LweI	GCATC	5 cut(s)	102, 159, 1336, 1585, 1916
MaeI	CTAG	1 cut(s)	1100
MaeII	ACGT	3 cut(s)	1060, 1150, 1865
MaeIII	GTNAC	3 cut(s)	1357, 1673, 1789
MfeI	CAATTG	1 cut(s)	726
MflI	RGATCY	7 cut(s)	228, 1138, 1369, 1852, 1897, 1957, 2020
MhlI	GDGCHC	1 cut(s)	1060
MlyI	GAGTC	6 cut(s)	1651, 1670, 1834, 1924, 2002, 2050
MmeI	TCCRAC	1 cut(s)	565
MroXI	GAANNNNTTC	3 cut(s)	10, 30, 749
MseI	TTAA	9 cut(s)	405, 846, 912, 989, 1194, 1212, 1491, 1554, 1688
MslI	CAYNNNNRTG	1 cut(s)	450
MspA1I	CMGCKG	1 cut(s)	1111
MspCI	CTTAAG	1 cut(s)	1687
MspI	CCGG	6 cut(s)	9, 413, 485, 606, 962, 1448
MspR9I	CCNGG	9 cut(s)	606, 702, 962, 963, 1277, 1448, 1707, 1857, 1902
MunI	CAATTG	1 cut(s)	726
MvaI	CCWGG	5 cut(s)	702, 1277, 1707, 1857, 1902
MvnI	CGCG	1 cut(s)	69
MwoI	GCNNNNNNNGC	4 cut(s)	178, 244, 1607, 1750
NciI	CCSGG	4 cut(s)	606, 962, 963, 1448
NlaIV	GGNNCC	2 cut(s)	618, 935
NmuCI	GTSAC	1 cut(s)	1673
NspI	RCATGY	2 cut(s)	959, 1508
NspV	TTCGAA	2 cut(s)	194, 260
PcsI	WCGNNNNNNNCGW	1 cut(s)	1066
PdmI	GAANNNNTTC	3 cut(s)	10, 30, 749
PfeI	GAWTC	4 cut(s)	31, 1031, 1049, 1766
PflMI	CCANNNNNTGG	1 cut(s)	1982
PfoI	TCCNGGA	5 cut(s)	700, 1275, 1446, 1855, 1900
PinAI	ACCGGT	1 cut(s)	8
PkrI	GCNGC	7 cut(s)	107, 121, 670, 1503, 1600, 1783, 1786
PleI	GAGTC	6 cut(s)	1650, 1670, 1834, 1924, 2002, 2050
PmaCI	CACGTG	1 cut(s)	1151
PmlI	CACGTG	1 cut(s)	1151
PpsI	GAGTC	6 cut(s)	1650, 1670, 1834, 1924, 2002, 2050
Ppu21I	YACGTR	1 cut(s)	1151
PpuMI	RGGWCCY	2 cut(s)	616, 934
Psp5II	RGGWCCY	2 cut(s)	616, 934
Psp6I	CCWGG	5 cut(s)	700, 1275, 1705, 1855, 1900
PspCI	CACGTG	1 cut(s)	1151
PspFI	CCCAGC	1 cut(s)	1111
PspGI	CCWGG	5 cut(s)	700, 1275, 1705, 1855, 1900
PspN4I	GGNNCC	2 cut(s)	618, 935
PspPI	GGNCC	4 cut(s)	44, 186, 616, 934
PspPPI	RGGWCCY	2 cut(s)	616, 934
PstI	CTGCAG	2 cut(s)	825, 1789
PsuI	RGATCY	7 cut(s)	228, 1138, 1369, 1852, 1897, 1957, 2020
PvuII	CAGCTG	1 cut(s)	1111
RsaI	GTAC	2 cut(s)	1119, 1125
RsaNI	GTAC	2 cut(s)	1118, 1124
RseI	CAYNNNNRTG	1 cut(s)	450
SaqAI	TTAA	9 cut(s)	405, 846, 912, 989, 1194, 1212, 1491, 1554, 1688
SatI	GCNGC	7 cut(s)	106, 120, 669, 1502, 1599, 1782, 1785
Sau96I	GGNCC	4 cut(s)	44, 186, 616, 934
SchI	GAGTC	6 cut(s)	1651, 1670, 1834, 1924, 2002, 2050
ScrFI	CCNGG	9 cut(s)	606, 702, 962, 963, 1277, 1448, 1707, 1857, 1902
SduI	GDGCHC	1 cut(s)	1060
SfaNI	GCATC	5 cut(s)	102, 159, 1336, 1585, 1916
SfcI	CTRYAG	3 cut(s)	342, 821, 1785
SfuI	TTCGAA	2 cut(s)	194, 260
SinI	GGWCC	3 cut(s)	44, 616, 934
SmaI	CCCGGG	1 cut(s)	963
SmiMI	CAYNNNNRTG	1 cut(s)	450
SmlI	CTYRAG	1 cut(s)	1687
SmoI	CTYRAG	1 cut(s)	1687
SsiI	CCGC	8 cut(s)	69, 105, 172, 199, 238, 456, 668, 1430
SspMI	CTAG	1 cut(s)	1100
StyD4I	CCNGG	9 cut(s)	604, 700, 960, 961, 1275, 1446, 1705, 1855, 1900
StyI	CCWWGG	2 cut(s)	89, 995
TaaI	ACNGT	3 cut(s)	1128, 1417, 1456
TaiI	ACGT	3 cut(s)	1063, 1153, 1868
TaqI	TCGA	7 cut(s)	24, 194, 254, 260, 558, 1037, 1711
TatI	WGTACW	1 cut(s)	1123
TauI	GCSGC	2 cut(s)	108, 671
TfiI	GAWTC	4 cut(s)	31, 1031, 1049, 1766
Tru1I	TTAA	9 cut(s)	405, 846, 912, 989, 1194, 1212, 1491, 1554, 1688
Tru9I	TTAA	9 cut(s)	405, 846, 912, 989, 1194, 1212, 1491, 1554, 1688
TscAI	CASTG	1 cut(s)	321
TseFI	GTSAC	1 cut(s)	1673
TseI	GCWGC	5 cut(s)	119, 1501, 1598, 1781, 1784
Tsp45I	GTSAC	1 cut(s)	1673
TspGWI	ACGGA	2 cut(s)	593, 1376
TspMI	CCCGGG	1 cut(s)	961
TspRI	CASTG	1 cut(s)	321
Van91I	CCANNNNNTGG	1 cut(s)	1982
Vha464I	CTTAAG	1 cut(s)	1687
VneI	GTGCAC	1 cut(s)	1056
VpaK11BI	GGWCC	3 cut(s)	44, 616, 934
XapI	RAATTY	3 cut(s)	211, 256, 1271
XceI	RCATGY	2 cut(s)	959, 1508
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1283
XmaI	CCCGGG	1 cut(s)	961
XmnI	GAANNNNTTC	3 cut(s)	10, 30, 749
XspI	CTAG	1 cut(s)	1100

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.