AT4G18195

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002718
Physical Location & Seq
Forward (+)
10069458 .. 10071115
1658 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18195.1

Sequence Viewer

Length: 1185 bp
ATGGAAATAACTCAAGTAATCTATGTCAATGATCATAACAACATAGAAGCAAACCTAACAGGTCAGGAGGAAATGAATACCACCATGGAAATCGAATCTTCGTCCGTACCTCAATCGAAGAACTATAAGAAATGGCTTCGTATTTCCATTTACGTGTTCTTTGTCCTTGCTTGCCAAGCACTTTCTACAATTTTGGGCAGAGTTTACTATGAAAATGGTGGGAAGAGTACATGGATGGGAACACTTGTCCAACTAATCGGCTTCCCTGTTCTGTTTCTCTTCCGCTTCTTTTCCCAAACCAAAAATCCCAAACCAACAGAAGCAGATTTCAGAAAGTTCTCTTCCTTCACCATTCTTGGATCAGTTTACATCGTTACTGGACTATTAGTGTCTGCTAACTCTTATATGTCCTCTGTTGGTTTACTATACTTACCAGTTTCTACTTTCTCCCTCATCTTGGCCTCACAATTGGCCTTCACTGCCTTCTTCTCATATTTTCTAAACTCGCAGAAGTTCACACCTTTCATTGTGAATTCTCTGTTTCTCCTTACTATTTCCTCTGCCCTCCTCGTGGTCAACACTGATTCGGAAAACACAGCAAAAGTGTCTAGAGTAAAATATGTGATAGGGATAATATGTACCATTGGTGCTTCTGCTGGGATTGGATTGCTGCTATCCCTGGTACAACTGATCCTCAGGAAGGTTTTAAAGAAGCAAACATTCTCAACGGTCACTGACTTGGTCGCTTACCAATCTCTAGTTGCAAGCTGTGTGGTTCTCATAGGACTTTTCGCAAGCGGGGAGTGGAAAACTTTAACAAGTGAGATGGAAAACTACAAACTGGGGAAAGTGCCATACGTTATGACTTTGGCCTCGATAGCTATTTCCTGGCAAGTCTACACCATTGGCGTCGTGGGACTGATCTTTGAGTCATCTTCTGTGTTCTCCAATTCCATAACTGCTGTGGGATTGCCTATAGTTCCAGTTGTAGCAGTGATTGTTTTCCATGATAAAATGAACGCGTCAAAGATCTTCTCCATCATTTTAGCTATCTGGGGATTCATTTCATTTGTCTATCAGCACTACCTCGACGAAAAGAAGTTGAAGACTAGCCACACAAGTCCTGTAGGAGATCCTCATCTACTACCTGCTGAGGAAGGTCACACAAACATACATAGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

394

Amino Acids

43.54

Weight (kDa)

8.75

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 47 - 367 8.1e-126 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1156
AccI GTMKAC 1 cut(s) 897
AccII CGCG 1 cut(s) 1022
AciI CCGC 2 cut(s) 283, 798
AclWI GGATC 3 cut(s) 367, 685, 1127
AcsI RAATTY 1 cut(s) 532
AcyI GRCGYC 1 cut(s) 909
AfaI GTAC 4 cut(s) 108, 229, 640, 684
AfiI CCNNNNNNNGG 3 cut(s) 457, 571, 700
AflIII ACRYGT 2 cut(s) 153, 1020
AgsI TTSAA 1 cut(s) 1105
AjnI CCWGG 2 cut(s) 678, 887
AluBI AGCT 3 cut(s) 768, 881, 1049
AluI AGCT 3 cut(s) 768, 881, 1049
AlwI GGATC 3 cut(s) 367, 685, 1127
AoxI GGCC 3 cut(s) 459, 471, 870
ApeKI GCWGC 1 cut(s) 670
ApoI RAATTY 1 cut(s) 532
AsuHPI GGTGA 1 cut(s) 340
AxyI CCTNAGG 1 cut(s) 695
BauI CACGAG 1 cut(s) 569
BbsI GAAGAC 1 cut(s) 1112
BbvCI CCTCAGC 1 cut(s) 1152
BbvI GCAGC 1 cut(s) 657
BccI CCATC 3 cut(s) 229, 820, 1046
BciT130I CCWGG 2 cut(s) 680, 889
BclI TGATCA 1 cut(s) 31
BfaI CTAG 3 cut(s) 609, 758, 1110
BfmI CTRYAG 2 cut(s) 975, 1125
BfuAI ACCTGC 1 cut(s) 1156
BglII AGATCT 1 cut(s) 1029
BisI GCNGC 1 cut(s) 671
BlsI GCNGC 1 cut(s) 672
Bme1390I CCNGG 2 cut(s) 680, 889
BmrFI CCNGG 2 cut(s) 680, 889
BmrI ACTGGG 1 cut(s) 851
BmuI ACTGGG 1 cut(s) 851
BpiI GAAGAC 1 cut(s) 1112
Bpu10I CCTNAGC 1 cut(s) 1152
BsaAI YACGTR 1 cut(s) 154
BsaBI GATNNNNATC 1 cut(s) 1137
BsaHI GRCGYC 1 cut(s) 909
BsaJI CCNNGG 2 cut(s) 84, 678
Bsc4I CCNNNNNNNGG 3 cut(s) 457, 571, 700
Bse1I ACTGG 4 cut(s) 382, 434, 846, 983
Bse21I CCTNAGG 1 cut(s) 695
Bse8I GATNNNNATC 1 cut(s) 1137
BseBI CCWGG 2 cut(s) 680, 889
BseDI CCNNGG 2 cut(s) 84, 678
BseGI GGATG 1 cut(s) 240
BseJI GATNNNNATC 1 cut(s) 1137
BseLI CCNNNNNNNGG 3 cut(s) 457, 571, 700
BseMII CTCAG 2 cut(s) 709, 1143
BseNI ACTGG 4 cut(s) 382, 434, 846, 983
BseRI GAGGAG 1 cut(s) 557
BseXI GCAGC 1 cut(s) 657
BseYI CCCAGC 1 cut(s) 656
Bsh1236I CGCG 1 cut(s) 1022
BshFI GGCC 3 cut(s) 461, 473, 872
BslFI GGGAC 1 cut(s) 930
BslI CCNNNNNNNGG 3 cut(s) 457, 571, 700
BsmFI GGGAC 1 cut(s) 930
BsnI GGCC 3 cut(s) 461, 473, 872
Bsp143I GATC 6 cut(s) 31, 359, 690, 921, 1029, 1132
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 2 cut(s) 283, 798
BspANI GGCC 3 cut(s) 461, 473, 872
BspCNI CTCAG 2 cut(s) 708, 1144
BspFNI CGCG 1 cut(s) 1022
BspMI ACCTGC 1 cut(s) 1156
BspPI GGATC 3 cut(s) 367, 685, 1127
BsrI ACTGG 4 cut(s) 382, 434, 846, 983
BssECI CCNNGG 2 cut(s) 84, 678
BssMI GATC 6 cut(s) 31, 359, 690, 921, 1029, 1132
BssNI GRCGYC 1 cut(s) 909
BssSI CACGAG 1 cut(s) 569
BssT1I CCWWGG 1 cut(s) 84
Bst2BI CACGAG 1 cut(s) 569
Bst2UI CCWGG 2 cut(s) 680, 889
Bst4CI ACNGT 1 cut(s) 730
Bst6I CTCTTC 3 cut(s) 218, 284, 346
BstACI GRCGYC 1 cut(s) 909
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 3 cut(s) 172, 766, 796
BstDEI CTNAG 2 cut(s) 695, 1152
BstDSI CCRYGG 1 cut(s) 84
BstENI CCTNNNNNAGG 1 cut(s) 698
BstF5I GGATG 1 cut(s) 240
BstFNI CGCG 1 cut(s) 1022
BstKTI GATC 6 cut(s) 34, 362, 693, 924, 1032, 1135
BstMBI GATC 6 cut(s) 31, 359, 690, 921, 1029, 1132
BstMWI GCNNNNNNNGC 3 cut(s) 176, 479, 878
BstNI CCWGG 2 cut(s) 680, 889
BstSCI CCNGG 2 cut(s) 678, 887
BstSFI CTRYAG 2 cut(s) 975, 1125
BstUI CGCG 1 cut(s) 1022
BstV1I GCAGC 1 cut(s) 657
BstV2I GAAGAC 1 cut(s) 1112
BstX2I RGATCY 2 cut(s) 1029, 1132
BstYI RGATCY 2 cut(s) 1029, 1132
Bsu36I CCTNAGG 1 cut(s) 695
BsuRI GGCC 3 cut(s) 461, 473, 872
BtgI CCRYGG 1 cut(s) 84
BtsCI GGATG 1 cut(s) 240
BtsI GCAGTG 2 cut(s) 477, 999
BtsIMutI CAGTG 4 cut(s) 477, 579, 732, 999
BveI ACCTGC 1 cut(s) 1156
Cac8I GCNNGC 3 cut(s) 172, 766, 796
CseI GACGC 2 cut(s) 898, 1011
Csp6I GTAC 4 cut(s) 107, 228, 639, 683
CspCI CAANNNNNGTGG 2 cut(s) 753, 788
CviAII CATG 3 cut(s) 85, 231, 1007
CviJI RGCY 9 cut(s) 136, 261, 461, 473, 768, 872, 881, 1049, 1113
CviKI_1 RGCY 9 cut(s) 136, 261, 461, 473, 768, 872, 881, 1049, 1113
CviQI GTAC 4 cut(s) 107, 228, 639, 683
DdeI CTNAG 2 cut(s) 695, 1152
DpnI GATC 6 cut(s) 33, 361, 692, 923, 1031, 1134
DpnII GATC 6 cut(s) 31, 359, 690, 921, 1029, 1132
DraI TTTAAA 1 cut(s) 708
Eam1104I CTCTTC 3 cut(s) 218, 284, 346
EarI CTCTTC 3 cut(s) 218, 284, 346
Eco130I CCWWGG 1 cut(s) 84
Eco81I CCTNAGG 1 cut(s) 695
EcoNI CCTNNNNNAGG 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 532
EcoRII CCWGG 2 cut(s) 678, 887
EcoT14I CCWWGG 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 3 cut(s) 88, 234, 1010
FaqI GGGAC 1 cut(s) 930
FatI CATG 3 cut(s) 84, 230, 1006
FauI CCCGC 1 cut(s) 791
FbaI TGATCA 1 cut(s) 31
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 1 cut(s) 671
FokI GGATG 1 cut(s) 247
Fsp4HI GCNGC 1 cut(s) 671
FspBI CTAG 3 cut(s) 609, 758, 1110
GluI GCNGC 1 cut(s) 671
GsaI CCCAGC 1 cut(s) 660
HaeIII GGCC 3 cut(s) 461, 473, 872
HgaI GACGC 2 cut(s) 898, 1011
Hin1I GRCGYC 1 cut(s) 909
Hin1II CATG 3 cut(s) 88, 234, 1010
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HinfI GANTC 4 cut(s) 95, 584, 929, 1059
HphI GGTGA 1 cut(s) 340
Hpy166II GTNNAC 6 cut(s) 205, 367, 422, 516, 577, 898
Hpy188I TCNGA 2 cut(s) 332, 589
Hpy188III TCNNGA 3 cut(s) 65, 609, 697
Hpy8I GTNNAC 6 cut(s) 205, 367, 422, 516, 577, 898
Hpy99I CGWCG 2 cut(s) 914, 1094
HpyAV CCTTC 5 cut(s) 355, 484, 493, 694, 1151
HpyCH4III ACNGT 1 cut(s) 730
HpyCH4IV ACGT 2 cut(s) 153, 858
HpyCH4V TGCA 1 cut(s) 764
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 479, 878
HpyF3I CTNAG 2 cut(s) 695, 1152
HpySE526I ACGT 2 cut(s) 153, 858
Hsp92I GRCGYC 1 cut(s) 909
Hsp92II CATG 3 cut(s) 88, 234, 1010
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 6 cut(s) 31, 359, 690, 921, 1029, 1132
Lsp1109I GCAGC 1 cut(s) 657
MaeI CTAG 3 cut(s) 609, 758, 1110
MaeII ACGT 2 cut(s) 153, 858
MaeIII GTNAC 3 cut(s) 373, 730, 1160
MalI GATC 6 cut(s) 33, 361, 692, 923, 1031, 1134
MboI GATC 6 cut(s) 31, 359, 690, 921, 1029, 1132
MboII GAAGA 9 cut(s) 90, 130, 235, 271, 333, 478, 927, 1024, 1117
MfeI CAATTG 1 cut(s) 467
MflI RGATCY 2 cut(s) 1029, 1132
MluCI AATT 4 cut(s) 189, 467, 532, 949
MluI ACGCGT 1 cut(s) 1020
MlyI GAGTC 1 cut(s) 938
MmeI TCCRAC 1 cut(s) 274
MseI TTAA 2 cut(s) 707, 815
MslI CAYNNNNRTG 1 cut(s) 152
MspR9I CCNGG 2 cut(s) 680, 889
MunI CAATTG 1 cut(s) 467
MvaI CCWGG 2 cut(s) 680, 889
MvnI CGCG 1 cut(s) 1022
MwoI GCNNNNNNNGC 3 cut(s) 176, 479, 878
NcoI CCATGG 1 cut(s) 84
NdeII GATC 6 cut(s) 31, 359, 690, 921, 1029, 1132
NlaIII CATG 3 cut(s) 88, 234, 1010
NmuCI GTSAC 2 cut(s) 730, 1160
PfeI GAWTC 3 cut(s) 95, 584, 1059
PflFI GACNNNGTC 1 cut(s) 740
PkrI GCNGC 1 cut(s) 672
PleI GAGTC 1 cut(s) 937
PpsI GAGTC 1 cut(s) 937
Ppu21I YACGTR 1 cut(s) 154
Psp6I CCWGG 2 cut(s) 678, 887
PspFI CCCAGC 1 cut(s) 656
PspGI CCWGG 2 cut(s) 678, 887
PsuI RGATCY 2 cut(s) 1029, 1132
PsyI GACNNNGTC 1 cut(s) 740
RsaI GTAC 4 cut(s) 108, 229, 640, 684
RsaNI GTAC 4 cut(s) 107, 228, 639, 683
RseI CAYNNNNRTG 1 cut(s) 152
SaqAI TTAA 2 cut(s) 707, 815
SatI GCNGC 1 cut(s) 671
Sau3AI GATC 6 cut(s) 31, 359, 690, 921, 1029, 1132
SchI GAGTC 1 cut(s) 938
ScrFI CCNGG 2 cut(s) 680, 889
SfcI CTRYAG 2 cut(s) 975, 1125
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
Sse9I AATT 4 cut(s) 189, 467, 532, 949
SsiI CCGC 2 cut(s) 283, 798
SspMI CTAG 3 cut(s) 609, 758, 1110
StyD4I CCNGG 2 cut(s) 678, 887
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 1 cut(s) 730
TaiI ACGT 2 cut(s) 156, 861
TaqI TCGA 4 cut(s) 93, 116, 875, 1089
TasI AATT 4 cut(s) 189, 467, 532, 949
TatI WGTACW 1 cut(s) 227
TfiI GAWTC 3 cut(s) 95, 584, 1059
Tru1I TTAA 2 cut(s) 707, 815
Tru9I TTAA 2 cut(s) 707, 815
TscAI CASTG 4 cut(s) 484, 586, 739, 999
TseFI GTSAC 2 cut(s) 730, 1160
TseI GCWGC 1 cut(s) 670
Tsp45I GTSAC 2 cut(s) 730, 1160
TspDTI ATGAA 6 cut(s) 89, 225, 514, 1031, 1051, 1056
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 4 cut(s) 484, 586, 739, 999
Tth111I GACNNNGTC 1 cut(s) 740
XagI CCTNNNNNAGG 1 cut(s) 698
XapI RAATTY 1 cut(s) 532
XbaI TCTAGA 1 cut(s) 608
XcmI CCANNNNNNNNNTGG 2 cut(s) 910, 961
XmiI GTMKAC 1 cut(s) 897
XspI CTAG 3 cut(s) 609, 758, 1110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.