Rorug05G0007600

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002718
Physical Location & Seq
Reverse (-)
442095 .. 448550
6456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0007600.1

Sequence Viewer

Length: 3057 bp
ATGGACATGTCAAAGGACAATCCAGGTGGAGTCGATCATAAGGACCTTGAATCTCAGCAAACAGTGTCAAAAGAGCTTGAACTGGTGCCCATTGATCCTAACCGCCGCTGGCGTACGATTGTGCTGATCCTTCAGGCACGCCATATTTTCATCACCTTAGCAGCTGCTAAAAATCCTGAACTTGGATCTCTTACAGGAGACTTGGAAGTAACTATAAGTACCCCTCATCCTACACCACCTGAAGGGGTTACCGCCAATGTATATTCCGACAACCCCAATGACAGTGAATATCAGCTCCAGTTTGCCACCATTTCCAACATCATAAAGACAAAGGATTTAACATCCTTACGTGACTTTGGAGGTGCCCAAGCATTTGCAGAGGCTCTCGGTAGTGATTTGGAGACTGGGATCCCCACCAACGATGAAGCTACTTGTTGTCGGCTCAAATCCACCACAAATTCCACTACTCCTGAACAGGCTGCTGCAAGAGGCTTCATTCAGCTAGCCAAAATATATTGCAACAGTTACATGATTCTCTTGCTTTTCGTGGCTGCTGTGCTCTCACTTTGCTTTGGAATCGTGGAGGAAGGCCCGACGACTGGTTGGTTTGAAGGGGCCATCATACTTTATGCCATTATCATGCTCGTGGTTCTTCCTTCCATACGTGATTATTGGCGGGAGTCTAAAGGGTTGTCCGGGAGGCAAAAACTGTTGGAGAACACAGATGAAGTGGAGGTTGAGGTCTTTAGAGGCGGAAATGAGGAGAAGATCTCCTCATCAGATATTTTGTTTGGTGACATCGTGTGCTTGAAGAGAGGACATCAGGTTCCTGCTGATGGTTTATTCATATCAGGTGGTTTCGTAGTCTTGGATGATCAATTAGGGGCAGTAGTCGATGCCGATAACCCTTTTCTCTTCTATGGAGCGGAAGTGACAGATGGCAATGGTCTCATGCTTGTGACATCTGTGGGTGTAAACACAGTGTGGGGTGACATGATGAGCAGCATAAACCATTTGAAGAAGACACCATTAGAGGCACAACTTGACAGGGTGAACACTTGTATGCAGATCATTGGTCTCCTAATCTCTATCCTAATCCTTGTGGTGTTGTTCTTGCGTTTTGAACTCAAAAAAGAACTGCACACCGGAGGATCCATGGATCTAAGGGGAGAGCCAACTAAAATGAACATGTTTATGGATGCCATCAAGAAAATTGTCATGAAACCAAGCGGAAAGATTAGTATCTTGACAACTTCTCTTACGGTGATGCTGGTTGGGATAGTAGAAGGGTTACCTTTCTGTATCAACCTTGCCATCATTTACTGGAACAGGAAGGCTTTGAATGGGATGGCCTCTCTTCAACACCTGTTGGCCTGTGTCACCATGGCTTCTGTTACATCTATCTGCACAGACAAAAACGGTGGGCTAACATTGGATTCAATGGAAGTTGACATGTGCTGGATTGGCGAACAAGTTATGGCTGAAAATTCTGTTATAGCCTCAAATGTCCTCGAAGCTTTTCGTGATGGAATCAGCATGCTCCTTCTCTCGCAGCCTCCTCCTTATACCTCAATAGAAGACCCAGTCATTTCCTGGGCGGCACTCAAGTTGGGCATGGAATTGGAAATTGTGAGGCAGAGTAATACCGTTGTCGAGGACAAAGAATTGACAACTGATCAGGAAGGAAGAGGAGCAATGATGCGGAAGAACAGAGACGGTGAAAGAGAAAGAATGTACTTGCATTGCAGAGGACCTGCTACCAGTATTTTGCCCATTTGTTCACACTACCATGATTGTGAAGGGATGATCAACGTCATCGAAGAACGAAACAGGGTGACTTTTCATAGAAATGACGAGGATATGCAGTCCAAGCATCTTAAAACCATGGCATTTGCGTACAAGGAAATTGATGTCCCAACAATGGAAGAAAGAGATCTAATATGGCTGGGACTGGTTGGTCTGAAGCCATGTTCGGAAAAGATGAGGGAATCAATCAGAGCCATAAGAAACGCTGGAGTCAATATCATACTGGTTTCCGGAGATGATGTGCAGACACTAGTGAGCATAGCTGAGCATTTCGGAATTCTGGGGGTGCGGAATTCAAATGAGATGGTGATCACAGGTGAAAAGTTCAGGAATTGCAGTGATGAAGACAGGATGAAGATGGTAAGTGAAATTTCAGTGATGGGAAACTCACTCCCCTCCGACAAGCTTCTTCTGGTTAAGTGTTTGAAGCTAAAAGGGGAAACAGTAGCAGTCATTGGAGTTAAAACGAATGAGGCTCCTGCACTGAAAGAAGCTGATGTTGGACTTACAATGGGAATATGGACCACCAAAATGGCTAAAGCGAGTTCAGACATCATCGTAAGGGTTGCAGATATCAGTTGCCTGGTAAGTATCCTCAGGTATGGAAGATGTGCCTATTACAACATTCAAAAGTACATCCAACTTGAGCTTACTATAGTCGCCGCAGGGCTTTTAGTAACATTTATCATAGCAGCATCTACAGGAAATATGCCCATAACAAGTATCCAATTGTTCTGGGCAAACCTGGTAGTGCCAATTCTGGGCGGCCTGGCTCTATTGACAGAGCTCCCAAGAAAGAAGTTAATGGAGAAATCACAATCCGGACAAACCAAAGCAATTATTACCATGGCCATGTGCAGAAATATAATAATCCAAGCTTCATATCAAGCTGTTACTATAGTCACCTTCCATTTCAAGGGACAAGCAATCTTGGGTGTTGATGAAAAGGTTCGCAAGACTATCATCTTCAATAGTTTTGTCATCTGCCAGATTTTTAACAAGTTCAGTGCGAGAGAGCTGGTAAAGAAAAATGTGTTCAAGGCGATCCACAAAAACCAATGGTTTTGGGTGGCTTTGGCTGCAATCCTAGTGCTACAGCTGAGTTTTATTGAGATTTCACATGTGGTTGCTGGCGATGCAAGGCTGAGCTTGGTGCACTGGGGTTTCTGCATTCTTATTGGGATTGTTTCGCTAGGGATGGATATAGCGGCAAAGGGCATATATGATTACATGTCATCTTTCGTCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

1018

Amino Acids

112.33

Weight (kDa)

5.63

Isoelectric Point (pI)

32.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1-E2_ATPase PF00122 244 - 336 2.2e-08 P-type ATPase actuator domain
Cation_ATPase PF13246 534 - 655 6.9e-09 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 658 - 768 2.1e-07 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 839 - 1007 6.5e-32 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1956
Acc36I ACCTGC 1 cut(s) 1762
AccB1I GGYRCC 2 cut(s) 85, 362
AccBSI CCGCTC 1 cut(s) 926
AccIII TCCGGA 2 cut(s) 2036, 2627
AclWI GGATC 9 cut(s) 89, 121, 193, 403, 416, 1146, 1159, 1167, 2845
AcoI YGGCCR 1 cut(s) 2655
AcsI RAATTY 5 cut(s) 457, 1486, 2082, 2098, 2175
AcuI CTGAAG 3 cut(s) 116, 261, 1982
AdeI CACNNNGTG 1 cut(s) 984
AfaI GTAC 5 cut(s) 115, 220, 1736, 1898, 2441
AfiI CCNNNNNNNGG 7 cut(s) 182, 242, 599, 836, 1921, 2567, 2722
AflIII ACRYGT 5 cut(s) 6, 1188, 1452, 2926, 3036
AhlI ACTAGT 1 cut(s) 2056
AjnI CCWGG 5 cut(s) 22, 1592, 2388, 2550, 2574
AjuI GAANNNNNNNTTGG 2 cut(s) 2289, 2321
AloI GAACNNNNNNTCC 2 cut(s) 810, 842
Alw21I GWGCWC 3 cut(s) 561, 2595, 2964
Alw26I GTCTC 5 cut(s) 192, 395, 953, 1082, 1707
Alw44I GTGCAC 1 cut(s) 2960
AlwI GGATC 9 cut(s) 89, 121, 193, 403, 416, 1146, 1159, 1167, 2845
Aor13HI TCCGGA 2 cut(s) 2036, 2627
AoxI GGCC 6 cut(s) 589, 615, 1350, 1371, 2572, 2655
ApaLI GTGCAC 1 cut(s) 2960
ApeKI GCWGC 9 cut(s) 161, 164, 479, 482, 551, 1002, 1552, 2498, 2885
ApoI RAATTY 5 cut(s) 457, 1486, 2082, 2098, 2175
Asp700I GAANNNNTTC 2 cut(s) 1518, 2754
AspS9I GGNCC 5 cut(s) 43, 590, 615, 1751, 2328
AsuC2I CCSGG 1 cut(s) 697
AsuNHI GCTAGC 1 cut(s) 502
AvaII GGWCC 3 cut(s) 43, 1751, 2328
AxyI CCTNAGG 1 cut(s) 2402
BaeGI GKGCMC 3 cut(s) 90, 367, 2964
BalI TGGCCA 1 cut(s) 2657
BamHI GGATCC 2 cut(s) 408, 1151
BanI GGYRCC 2 cut(s) 85, 362
BanII GRGCYC 1 cut(s) 2595
BauI CACGAG 1 cut(s) 644
BbsI GAAGAC 3 cut(s) 1028, 1584, 2157
Bbv12I GWGCWC 3 cut(s) 561, 2595, 2964
BbvI GCAGC 9 cut(s) 151, 173, 466, 469, 538, 1014, 1564, 2510, 2872
BciT130I CCWGG 5 cut(s) 24, 1594, 2390, 2552, 2576
BciVI GTATCC 2 cut(s) 2408, 2540
BclI TGATCA 4 cut(s) 874, 1675, 1806, 2115
BcnI CCSGG 1 cut(s) 697
BcoDI GTCTC 5 cut(s) 192, 395, 953, 1082, 1707
BcuI ACTAGT 1 cut(s) 2056
BfaI CTAG 4 cut(s) 503, 2057, 2894, 2999
BfmI CTRYAG 4 cut(s) 2460, 2505, 2703, 2900
BfuAI ACCTGC 1 cut(s) 1762
BfuI GTATCC 2 cut(s) 2408, 2540
BglII AGATCT 2 cut(s) 768, 1933
BlpI GCTNAGC 2 cut(s) 2070, 2951
Bme1390I CCNGG 6 cut(s) 24, 697, 1594, 2390, 2552, 2576
Bme18I GGWCC 3 cut(s) 43, 1751, 2328
BmgT120I GGNCC 5 cut(s) 43, 590, 615, 1751, 2328
BmiI GGNNCC 7 cut(s) 87, 364, 410, 616, 828, 1153, 2283
BmrFI CCNGG 6 cut(s) 24, 697, 1594, 2390, 2552, 2576
BmrI ACTGGG 3 cut(s) 414, 1577, 2974
BmsI GCATC 7 cut(s) 886, 1189, 1257, 1689, 1882, 2510, 2932
BmtI GCTAGC 1 cut(s) 506
BmuI ACTGGG 3 cut(s) 414, 1577, 2974
BpiI GAAGAC 3 cut(s) 1028, 1584, 2157
BplI GAGNNNNNCTC 2 cut(s) 755, 787
BpmI CTGGAG 2 cut(s) 281, 2034
Bpu10I CCTNAGC 1 cut(s) 157
Bpu1102I GCTNAGC 2 cut(s) 2070, 2951
BpuEI CTTGAG 2 cut(s) 1589, 2471
BpuMI CCSGG 1 cut(s) 697
BsaAI YACGTR 2 cut(s) 350, 665
BsaBI GATNNNNATC 2 cut(s) 1241, 2114
BsaI GGTCTC 2 cut(s) 953, 1082
BsaJI CCNNGG 5 cut(s) 1155, 1383, 1593, 1884, 2652
BsaWI WCCGGW 3 cut(s) 1145, 2036, 2627
BsaXI ACNNNNNCTCC 4 cut(s) 915, 945, 2031, 2061
Bsc4I CCNNNNNNNGG 7 cut(s) 182, 242, 599, 836, 1921, 2567, 2722
Bse21I CCTNAGG 1 cut(s) 2402
Bse3DI GCAATG 3 cut(s) 949, 1701, 1741
Bse8I GATNNNNATC 2 cut(s) 1241, 2114
BseAI TCCGGA 2 cut(s) 2036, 2627
BseBI CCWGG 5 cut(s) 24, 1594, 2390, 2552, 2576
BseDI CCNNGG 5 cut(s) 1155, 1383, 1593, 1884, 2652
BseGI GGATG 9 cut(s) 226, 341, 877, 1204, 1353, 1809, 2163, 2442, 3009
BseJI GATNNNNATC 2 cut(s) 1241, 2114
BseLI CCNNNNNNNGG 7 cut(s) 182, 242, 599, 836, 1921, 2567, 2722
BseMI GCAATG 3 cut(s) 949, 1701, 1741
BseMII CTCAG 5 cut(s) 68, 2061, 2416, 2897, 2942
BseRI GAGGAG 4 cut(s) 763, 776, 1548, 1704
BseSI GKGCMC 3 cut(s) 90, 367, 2964
BseXI GCAGC 9 cut(s) 151, 173, 466, 469, 538, 1014, 1564, 2510, 2872
BseYI CCCAGC 1 cut(s) 1945
BsgI GTGCAG 5 cut(s) 1124, 1390, 2069, 2271, 2683
BshFI GGCC 6 cut(s) 591, 617, 1352, 1373, 2574, 2657
BshNI GGYRCC 2 cut(s) 85, 362
BsiHKAI GWGCWC 3 cut(s) 561, 2595, 2964
BsiSI CCGG 4 cut(s) 696, 1146, 2037, 2628
BsiWI CGTACG 1 cut(s) 113
BslFI GGGAC 3 cut(s) 1898, 1962, 2739
BslI CCNNNNNNNGG 7 cut(s) 182, 242, 599, 836, 1921, 2567, 2722
BsmAI GTCTC 5 cut(s) 192, 395, 953, 1082, 1707
BsmBI CGTCTC 1 cut(s) 1707
BsmFI GGGAC 3 cut(s) 1898, 1962, 2739
BsmI GAATGC 1 cut(s) 2976
BsnI GGCC 6 cut(s) 591, 617, 1352, 1373, 2574, 2657
Bso31I GGTCTC 2 cut(s) 953, 1082
Bsp1286I GDGCHC 5 cut(s) 90, 367, 561, 2595, 2964
Bsp13I TCCGGA 2 cut(s) 2036, 2627
Bsp1720I GCTNAGC 2 cut(s) 2070, 2951
Bsp19I CCATGG 4 cut(s) 1155, 1383, 1884, 2652
BspANI GGCC 6 cut(s) 591, 617, 1352, 1373, 2574, 2657
BspCNI CTCAG 5 cut(s) 67, 2062, 2415, 2898, 2943
BspEI TCCGGA 2 cut(s) 2036, 2627
BspHI TCATGA 1 cut(s) 1218
BspLI GGNNCC 7 cut(s) 87, 364, 410, 616, 828, 1153, 2283
BspMI ACCTGC 1 cut(s) 1762
BspOI GCTAGC 1 cut(s) 506
BspPI GGATC 9 cut(s) 89, 121, 193, 403, 416, 1146, 1159, 1167, 2845
BspT107I GGYRCC 2 cut(s) 85, 362
BspTNI GGTCTC 2 cut(s) 953, 1082
BsrBI CCGCTC 1 cut(s) 926
BsrDI GCAATG 3 cut(s) 949, 1701, 1741
BssECI CCNNGG 5 cut(s) 1155, 1383, 1593, 1884, 2652
BssSI CACGAG 1 cut(s) 644
BssT1I CCWWGG 4 cut(s) 1155, 1383, 1884, 2652
Bst2BI CACGAG 1 cut(s) 644
Bst2UI CCWGG 5 cut(s) 24, 1594, 2390, 2552, 2576
Bst6I CTCTTC 4 cut(s) 806, 920, 1362, 1681
BstBAI YACGTR 2 cut(s) 350, 665
BstC8I GCNNGC 5 cut(s) 110, 139, 504, 1538, 2938
BstDEI CTNAG 7 cut(s) 54, 157, 1163, 2070, 2402, 2906, 2951
BstDSI CCRYGG 4 cut(s) 1155, 1383, 1884, 2652
BstEII GGTNACC 2 cut(s) 247, 1290
BstF5I GGATG 9 cut(s) 226, 341, 877, 1204, 1353, 1809, 2163, 2442, 3009
BstMAI GTCTC 5 cut(s) 192, 395, 953, 1082, 1707
BstMWI GCNNNNNNNGC 4 cut(s) 1464, 1870, 2885, 2942
BstNI CCWGG 5 cut(s) 24, 1594, 2390, 2552, 2576
BstNSI RCATGY 6 cut(s) 10, 1192, 1456, 1540, 2930, 3040
BstPI GGTNACC 2 cut(s) 247, 1290
BstSCI CCNGG 6 cut(s) 22, 695, 1592, 2388, 2550, 2574
BstSFI CTRYAG 4 cut(s) 2460, 2505, 2703, 2900
BstSLI GKGCMC 3 cut(s) 90, 367, 2964
BstV1I GCAGC 9 cut(s) 151, 173, 466, 469, 538, 1014, 1564, 2510, 2872
BstV2I GAAGAC 3 cut(s) 1028, 1584, 2157
BstX2I RGATCY 6 cut(s) 185, 408, 768, 1151, 1159, 1933
BstXI CCANNNNNNTGG 1 cut(s) 2338
BstYI RGATCY 6 cut(s) 185, 408, 768, 1151, 1159, 1933
Bsu36I CCTNAGG 1 cut(s) 2402
BsuI GTATCC 2 cut(s) 2408, 2540
BsuRI GGCC 6 cut(s) 591, 617, 1352, 1373, 2574, 2657
BtgI CCRYGG 4 cut(s) 1155, 1383, 1884, 2652
BtgZI GCGATG 1 cut(s) 2955
BtsCI GGATG 9 cut(s) 226, 341, 877, 1204, 1353, 1809, 2163, 2442, 3009
BtsI GCAGTG 1 cut(s) 2149
BtsIMutI CAGTG 8 cut(s) 69, 289, 987, 2149, 2187, 2288, 2818, 2962
BveI ACCTGC 1 cut(s) 1762
Cac8I GCNNGC 5 cut(s) 110, 139, 504, 1538, 2938
CciI TCATGA 1 cut(s) 1218
Cfr13I GGNCC 5 cut(s) 43, 590, 615, 1751, 2328
CsiI ACCWGGT 1 cut(s) 2550
Csp6I GTAC 5 cut(s) 114, 219, 1735, 1897, 2440
CspCI CAANNNNNGTGG 4 cut(s) 7, 42, 1402, 1437
CviQI GTAC 5 cut(s) 114, 219, 1735, 1897, 2440
DdeI CTNAG 7 cut(s) 54, 157, 1163, 2070, 2402, 2906, 2951
DraIII CACNNNGTG 1 cut(s) 984
DrdI GACNNNNNNGTC 1 cut(s) 1956
DseDI GACNNNNNNGTC 1 cut(s) 1956
EaeI YGGCCR 1 cut(s) 2655
Eam1104I CTCTTC 4 cut(s) 806, 920, 1362, 1681
EarI CTCTTC 4 cut(s) 806, 920, 1362, 1681
EciI GGCGGA 1 cut(s) 768
Ecl136II GAGCTC 1 cut(s) 2593
Eco130I CCWWGG 4 cut(s) 1155, 1383, 1884, 2652
Eco24I GRGCYC 1 cut(s) 2595
Eco31I GGTCTC 2 cut(s) 953, 1082
Eco32I GATATC 1 cut(s) 2380
Eco47I GGWCC 3 cut(s) 43, 1751, 2328
Eco53kI GAGCTC 1 cut(s) 2593
Eco57I CTGAAG 3 cut(s) 116, 261, 1982
Eco81I CCTNAGG 1 cut(s) 2402
Eco91I GGTNACC 2 cut(s) 247, 1290
EcoICRI GAGCTC 1 cut(s) 2593
EcoO109I RGGNCCY 2 cut(s) 43, 1751
EcoO65I GGTNACC 2 cut(s) 247, 1290
EcoRI GAATTC 2 cut(s) 2082, 2098
EcoRII CCWGG 5 cut(s) 22, 1592, 2388, 2550, 2574
EcoRV GATATC 1 cut(s) 2380
EcoT14I CCWWGG 4 cut(s) 1155, 1383, 1884, 2652
EcoT38I GRGCYC 1 cut(s) 2595
ErhI CCWWGG 4 cut(s) 1155, 1383, 1884, 2652
Esp3I CGTCTC 1 cut(s) 1707
FaqI GGGAC 3 cut(s) 1898, 1962, 2739
FauI CCCGC 1 cut(s) 669
FbaI TGATCA 4 cut(s) 874, 1675, 1806, 2115
FokI GGATG 9 cut(s) 213, 328, 884, 1211, 1360, 1816, 2170, 2429, 3016
FriOI GRGCYC 1 cut(s) 2595
FspBI CTAG 4 cut(s) 503, 2057, 2894, 2999
GsaI CCCAGC 1 cut(s) 1949
GsuI CTGGAG 2 cut(s) 281, 2034
HaeIII GGCC 6 cut(s) 591, 617, 1352, 1373, 2574, 2657
HapII CCGG 4 cut(s) 696, 1146, 2037, 2628
HincII GTYRAC 1 cut(s) 1450
HindII GTYRAC 1 cut(s) 1450
HindIII AAGCTT 3 cut(s) 1515, 2210, 2682
HinfI GANTC 9 cut(s) 30, 50, 532, 576, 680, 1436, 1530, 1988, 2016
HpaII CCGG 4 cut(s) 696, 1146, 2037, 2628
Hpy166II GTNNAC 5 cut(s) 976, 1054, 1450, 1781, 2962
Hpy188I TCNGA 8 cut(s) 268, 781, 1962, 1975, 1997, 2081, 2206, 2356
Hpy8I GTNNAC 5 cut(s) 976, 1054, 1450, 1781, 2962
Hpy99I CGWCG 2 cut(s) 598, 3053
HpyCH4IV ACGT 3 cut(s) 349, 664, 1812
HpyF10VI GCNNNNNNNGC 4 cut(s) 1464, 1870, 2885, 2942
HpyF3I CTNAG 7 cut(s) 54, 157, 1163, 2070, 2402, 2906, 2951
HpySE526I ACGT 3 cut(s) 349, 664, 1812
Kpn2I TCCGGA 2 cut(s) 2036, 2627
Ksp22I TGATCA 4 cut(s) 874, 1675, 1806, 2115
LmnI GCTCC 6 cut(s) 300, 923, 1545, 1691, 2287, 2598
Lsp1109I GCAGC 9 cut(s) 151, 173, 466, 469, 538, 1014, 1564, 2510, 2872
LweI GCATC 7 cut(s) 886, 1189, 1257, 1689, 1882, 2510, 2932
MabI ACCWGGT 1 cut(s) 2550
MaeI CTAG 4 cut(s) 503, 2057, 2894, 2999
MaeII ACGT 3 cut(s) 349, 664, 1812
MbiI CCGCTC 1 cut(s) 926
MfeI CAATTG 1 cut(s) 2534
MflI RGATCY 6 cut(s) 185, 408, 768, 1151, 1159, 1933
MhlI GDGCHC 5 cut(s) 90, 367, 561, 2595, 2964
MlsI TGGCCA 1 cut(s) 2657
MluNI TGGCCA 1 cut(s) 2657
MlyI GAGTC 3 cut(s) 39, 689, 2025
MmeI TCCRAC 6 cut(s) 291, 339, 693, 2229, 2287, 2470
Mox20I TGGCCA 1 cut(s) 2657
MroI TCCGGA 2 cut(s) 2036, 2627
MroXI GAANNNNTTC 2 cut(s) 1518, 2754
MscI TGGCCA 1 cut(s) 2657
MseI TTAA 6 cut(s) 338, 1878, 2223, 2268, 2609, 2802
Msp20I TGGCCA 1 cut(s) 2657
MspA1I CMGCKG 3 cut(s) 108, 164, 2905
MspI CCGG 4 cut(s) 696, 1146, 2037, 2628
MspR9I CCNGG 6 cut(s) 24, 697, 1594, 2390, 2552, 2576
MunI CAATTG 1 cut(s) 2534
Mva1269I GAATGC 1 cut(s) 2976
MvaI CCWGG 5 cut(s) 24, 1594, 2390, 2552, 2576
MwoI GCNNNNNNNGC 4 cut(s) 1464, 1870, 2885, 2942
NciI CCSGG 1 cut(s) 697
NcoI CCATGG 4 cut(s) 1155, 1383, 1884, 2652
NheI GCTAGC 1 cut(s) 502
NlaIV GGNNCC 7 cut(s) 87, 364, 410, 616, 828, 1153, 2283
NmuCI GTSAC 8 cut(s) 350, 794, 931, 958, 989, 1378, 1834, 2707
NspI RCATGY 6 cut(s) 10, 1192, 1456, 1540, 2930, 3040
PaeI GCATGC 1 cut(s) 1540
PagI TCATGA 1 cut(s) 1218
PciI ACATGT 5 cut(s) 6, 1188, 1452, 2926, 3036
PctI GAATGC 1 cut(s) 2976
PdmI GAANNNNTTC 2 cut(s) 1518, 2754
PfeI GAWTC 6 cut(s) 50, 532, 576, 1436, 1530, 1988
Pfl23II CGTACG 1 cut(s) 113
PflFI GACNNNGTC 1 cut(s) 1583
PfoI TCCNGGA 1 cut(s) 695
PleI GAGTC 3 cut(s) 38, 688, 2024
PpsI GAGTC 3 cut(s) 38, 688, 2024
Ppu21I YACGTR 2 cut(s) 350, 665
PpuMI RGGWCCY 2 cut(s) 43, 1751
PscI ACATGT 5 cut(s) 6, 1188, 1452, 2926, 3036
Psp124BI GAGCTC 1 cut(s) 2595
Psp5II RGGWCCY 2 cut(s) 43, 1751
Psp6I CCWGG 5 cut(s) 22, 1592, 2388, 2550, 2574
PspEI GGTNACC 2 cut(s) 247, 1290
PspFI CCCAGC 1 cut(s) 1945
PspGI CCWGG 5 cut(s) 22, 1592, 2388, 2550, 2574
PspLI CGTACG 1 cut(s) 113
PspN4I GGNNCC 7 cut(s) 87, 364, 410, 616, 828, 1153, 2283
PspPI GGNCC 5 cut(s) 43, 590, 615, 1751, 2328
PspPPI RGGWCCY 2 cut(s) 43, 1751
PsuI RGATCY 6 cut(s) 185, 408, 768, 1151, 1159, 1933
PsyI GACNNNGTC 1 cut(s) 1583
PvuII CAGCTG 2 cut(s) 164, 2905
RsaI GTAC 5 cut(s) 115, 220, 1736, 1898, 2441
RsaNI GTAC 5 cut(s) 114, 219, 1735, 1897, 2440
SacI GAGCTC 1 cut(s) 2595
SaqAI TTAA 6 cut(s) 338, 1878, 2223, 2268, 2609, 2802
Sau96I GGNCC 5 cut(s) 43, 590, 615, 1751, 2328
SchI GAGTC 3 cut(s) 39, 689, 2025
ScrFI CCNGG 6 cut(s) 24, 697, 1594, 2390, 2552, 2576
SduI GDGCHC 5 cut(s) 90, 367, 561, 2595, 2964
SexAI ACCWGGT 1 cut(s) 2550
SfaNI GCATC 7 cut(s) 886, 1189, 1257, 1689, 1882, 2510, 2932
SfcI CTRYAG 4 cut(s) 2460, 2505, 2703, 2900
SinI GGWCC 3 cut(s) 43, 1751, 2328
SmlI CTYRAG 2 cut(s) 1604, 2450
SmoI CTYRAG 2 cut(s) 1604, 2450
SpeI ACTAGT 1 cut(s) 2056
SphI GCATGC 1 cut(s) 1540
SspMI CTAG 4 cut(s) 503, 2057, 2894, 2999
SstI GAGCTC 1 cut(s) 2595
StyD4I CCNGG 6 cut(s) 22, 695, 1592, 2388, 2550, 2574
StyI CCWWGG 4 cut(s) 1155, 1383, 1884, 2652
TaiI ACGT 3 cut(s) 352, 667, 1815
TaqI TCGA 6 cut(s) 33, 894, 1512, 1653, 1818, 3051
TatI WGTACW 2 cut(s) 1734, 2439
TauI GCSGC 5 cut(s) 108, 1601, 2471, 2574, 3017
TfiI GAWTC 6 cut(s) 50, 532, 576, 1436, 1530, 1988
Tru1I TTAA 6 cut(s) 338, 1878, 2223, 2268, 2609, 2802
Tru9I TTAA 6 cut(s) 338, 1878, 2223, 2268, 2609, 2802
TscAI CASTG 8 cut(s) 69, 289, 987, 2149, 2187, 2295, 2818, 2969
TseFI GTSAC 8 cut(s) 350, 794, 931, 958, 989, 1378, 1834, 2707
TseI GCWGC 9 cut(s) 161, 164, 479, 482, 551, 1002, 1552, 2498, 2885
Tsp45I GTSAC 8 cut(s) 350, 794, 931, 958, 989, 1378, 1834, 2707
TspRI CASTG 8 cut(s) 69, 289, 987, 2149, 2187, 2295, 2818, 2969
Tth111I GACNNNGTC 1 cut(s) 1583
VneI GTGCAC 1 cut(s) 2960
VpaK11BI GGWCC 3 cut(s) 43, 1751, 2328
XapI RAATTY 5 cut(s) 457, 1486, 2082, 2098, 2175
XceI RCATGY 6 cut(s) 10, 1192, 1456, 1540, 2930, 3040
XcmI CCANNNNNNNNNTGG 1 cut(s) 1590
XmnI GAANNNNTTC 2 cut(s) 1518, 2754
XspI CTAG 4 cut(s) 503, 2057, 2894, 2999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.