MD10G1265700.v1.1

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002718
Physical Location & Seq
Reverse (-)
35903023 .. 35904636
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1265700.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGAGAATTAAGGAAGGAAATGGAGAAAGGGAAGCAAACTTATCAGGGCCTACACATGTCTCCGACCGATCACTGCTACCGCAGCGCAGAAGTGTCAACTGGTGGATTAGAATCGCCATTTACTCGTTCCTTGTCATTGCCGGCCAGTCAATTGCAACATTCTTGGGAAGACAGTACTATGACAAAGGCGCCAAAAGTAAATGGATGGCAACAGTAGTACAACTTTGTGGCTTTCCAATCATGCTTCCCTACTATTGCATCCCAGCAAGCAGAAATAATCCAATCCCAAAAGATAGTCCTATCCCATCAAAATCACCGTCTACCAAAATTCTCGCATCAGTATATGTCTCTCTTGGCATCCTAATAGCCCTAGACTGCTACTTGTATTCCGTAGGACTATCTTACCTTCCTGTATCTACTTTTACCCTCATTTGTGCATCTCAATTGGCCTTCAACGCGTTTTTCTCCTTCTTCCTCAACGCGCAAAAGTTCACTTCTTACATTGTCAATTCTCTAGTTCTCCTCACCATCTCCTCCACCCTCCTTGTGTTCCAAGGTGAAGACGACTCTGGAGACGATGATCCAAGTGGAGGATTGAAGGCAAAGTATGCAATTGGGTTCATATGCACCTTAGGGGCATCAGCCGGAGCCGGATTATTGCTTTCCCTAACTCAGCTTGCCTTCAGAAGGGTTATAAAACAGGGGACGTTTAGAGCGGTCATGGACCTGATAGTGTGTGAAAATATGGTCGCAACCTGTGTTACCGCCGTCGGACTTTTTGCTAGCGGAGAGTGGAAGCATTTGAACGGTGAGATGGAGGGGTATAAATTAGGGAAGATATCCTATGTGATGAATGTGACTTGGACAGCAATAACATGGCAGCTTTTTTCAGTTGGAACGGTAGGCTTGATTTTGGAGGCCTCTTCCCTCTTCTCCAATTCCATAAGTGCTTTGGGTTTGCCTGCTGTTCCAGTTTTAGCTGTGATCTTCTTCCATGAAAAAATGAGTGGAATTAAGGGAATAGCCATGGTTTTGGGTATTTGGGGATTTGTTTCCTACGCGTATCATCACTACATCGAAGATCGTAAGTCCAAGGCGGAGAATAGAAATTCCAGGAACGTTGAAGTTCCGAAAGCTTTAAATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

383

Amino Acids

41.82

Weight (kDa)

9.03

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 36 - 363 3.3e-120 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
AccB1I GGYRCC 1 cut(s) 188
AccBSI CCGCTC 1 cut(s) 716
AccI GTMKAC 1 cut(s) 320
AccII CGCG 3 cut(s) 458, 482, 1061
AciI CCGC 5 cut(s) 80, 716, 765, 786, 1097
AclI AACGTT 1 cut(s) 1119
AclWI GGATC 1 cut(s) 575
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 2 cut(s) 327, 1108
AcuI CTGAAG 1 cut(s) 667
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 2 cut(s) 176, 219
AfiI CCNNNNNNNGG 2 cut(s) 590, 687
AflIII ACRYGT 3 cut(s) 55, 456, 1059
AgsI TTSAA 4 cut(s) 454, 598, 805, 1124
AjnI CCWGG 1 cut(s) 1112
AluBI AGCT 4 cut(s) 676, 883, 980, 1136
AluI AGCT 4 cut(s) 676, 883, 980, 1136
Alw26I GTCTC 3 cut(s) 64, 352, 567
AlwI GGATC 1 cut(s) 575
AoxI GGCC 4 cut(s) 47, 142, 447, 918
ApeKI GCWGC 2 cut(s) 82, 880
ApoI RAATTY 2 cut(s) 327, 1108
ArsI GACNNNNNNTTYG 2 cut(s) 302, 334
AspLEI GCGC 3 cut(s) 87, 191, 484
AspS9I GGNCC 2 cut(s) 47, 724
AsuHPI GGTGA 4 cut(s) 306, 517, 569, 821
AsuNHI GCTAGC 1 cut(s) 782
AvaII GGWCC 1 cut(s) 724
AxyI CCTNAGG 1 cut(s) 631
BanI GGYRCC 1 cut(s) 188
BbsI GAAGAC 2 cut(s) 175, 567
BbvI GCAGC 2 cut(s) 94, 892
BccI CCATC 4 cut(s) 199, 313, 536, 808
BceAI ACGGC 1 cut(s) 752
BciT130I CCWGG 1 cut(s) 1114
BcoDI GTCTC 3 cut(s) 64, 352, 567
BfaI CTAG 3 cut(s) 371, 515, 783
BfoI RGCGCY 1 cut(s) 192
BisI GCNGC 2 cut(s) 83, 881
BlsI GCNGC 2 cut(s) 84, 882
BmcAI AGTACT 1 cut(s) 176
Bme1390I CCNGG 1 cut(s) 1114
Bme18I GGWCC 1 cut(s) 724
BmgT120I GGNCC 2 cut(s) 47, 724
BmiI GGNNCC 2 cut(s) 190, 649
BmrFI CCNGG 1 cut(s) 1114
BmsI GCATC 5 cut(s) 267, 344, 366, 446, 647
BmtI GCTAGC 1 cut(s) 786
BpiI GAAGAC 2 cut(s) 175, 567
BpmI CTGGAG 1 cut(s) 591
BsaBI GATNNNNATC 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 3 cut(s) 553, 1026, 1092
Bsc4I CCNNNNNNNGG 2 cut(s) 590, 687
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 3 cut(s) 104, 145, 971
Bse21I CCTNAGG 1 cut(s) 631
Bse3DI GCAATG 1 cut(s) 135
Bse8I GATNNNNATC 1 cut(s) 110
BseBI CCWGG 1 cut(s) 1114
BseDI CCNNGG 3 cut(s) 553, 1026, 1092
BseGI GGATG 3 cut(s) 210, 258, 357
BseJI GATNNNNATC 1 cut(s) 110
BseLI CCNNNNNNNGG 2 cut(s) 590, 687
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 1 cut(s) 686
BseNI ACTGG 3 cut(s) 104, 145, 971
BseRI GAGGAG 2 cut(s) 512, 523
BseXI GCAGC 2 cut(s) 94, 892
BseYI CCCAGC 1 cut(s) 262
Bsh1236I CGCG 3 cut(s) 458, 482, 1061
Bsh1285I CGRYCG 1 cut(s) 67
BshFI GGCC 4 cut(s) 49, 144, 449, 920
BshNI GGYRCC 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 67
BsiSI CCGG 3 cut(s) 141, 645, 651
BslFI GGGAC 1 cut(s) 718
BslI CCNNNNNNNGG 2 cut(s) 590, 687
BsmAI GTCTC 3 cut(s) 64, 352, 567
BsmBI CGTCTC 1 cut(s) 567
BsmFI GGGAC 1 cut(s) 718
BsnI GGCC 4 cut(s) 49, 144, 449, 920
Bsp143I GATC 4 cut(s) 68, 580, 984, 1081
Bsp19I CCATGG 1 cut(s) 1026
BspACI CCGC 5 cut(s) 80, 716, 765, 786, 1097
BspANI GGCC 4 cut(s) 49, 144, 449, 920
BspCNI CTCAG 1 cut(s) 685
BspFNI CGCG 3 cut(s) 458, 482, 1061
BspLI GGNNCC 2 cut(s) 190, 649
BspOI GCTAGC 1 cut(s) 786
BspPI GGATC 1 cut(s) 575
BspT107I GGYRCC 1 cut(s) 188
BsrBI CCGCTC 1 cut(s) 716
BsrDI GCAATG 1 cut(s) 135
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 3 cut(s) 104, 145, 971
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 3 cut(s) 553, 1026, 1092
BssMI GATC 4 cut(s) 68, 580, 984, 1081
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 3 cut(s) 553, 1026, 1092
Bst2UI CCWGG 1 cut(s) 1114
Bst4CI ACNGT 5 cut(s) 174, 214, 318, 809, 901
Bst6I CTCTTC 2 cut(s) 928, 935
BstACI GRCGYC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 608
BstC8I GCNNGC 5 cut(s) 142, 268, 678, 784, 963
BstDEI CTNAG 2 cut(s) 631, 672
BstDSI CCRYGG 1 cut(s) 1026
BstENI CCTNNNNNAGG 1 cut(s) 685
BstF5I GGATG 3 cut(s) 210, 258, 357
BstFNI CGCG 3 cut(s) 458, 482, 1061
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 3 cut(s) 87, 191, 484
BstKTI GATC 4 cut(s) 71, 583, 987, 1084
BstMAI GTCTC 3 cut(s) 64, 352, 567
BstMBI GATC 4 cut(s) 68, 580, 984, 1081
BstMCI CGRYCG 1 cut(s) 67
BstMWI GCNNNNNNNGC 3 cut(s) 82, 455, 608
BstNI CCWGG 1 cut(s) 1114
BstNSI RCATGY 1 cut(s) 59
BstSCI CCNGG 1 cut(s) 1112
BstUI CGCG 3 cut(s) 458, 482, 1061
BstV1I GCAGC 2 cut(s) 94, 892
BstV2I GAAGAC 2 cut(s) 175, 567
BstXI CCANNNNNNTGG 1 cut(s) 1033
Bsu36I CCTNAGG 1 cut(s) 631
BsuRI GGCC 4 cut(s) 49, 144, 449, 920
BtgI CCRYGG 1 cut(s) 1026
BtsCI GGATG 3 cut(s) 210, 258, 357
BtsI GCAGTG 1 cut(s) 71
BtsIMutI CAGTG 1 cut(s) 71
Cac8I GCNNGC 5 cut(s) 142, 268, 678, 784, 963
CfoI GCGC 3 cut(s) 87, 191, 484
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 2 cut(s) 47, 724
Csp6I GTAC 2 cut(s) 175, 218
CspCI CAANNNNNGTGG 2 cut(s) 526, 561
CviAII CATG 6 cut(s) 56, 241, 721, 876, 995, 1027
CviQI GTAC 2 cut(s) 175, 218
DdeI CTNAG 2 cut(s) 631, 672
DinI GGCGCC 1 cut(s) 190
DpnI GATC 4 cut(s) 70, 582, 986, 1083
DpnII GATC 4 cut(s) 68, 580, 984, 1081
DraI TTTAAA 1 cut(s) 1140
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 2 cut(s) 928, 935
EarI CTCTTC 2 cut(s) 928, 935
EciI GGCGGA 1 cut(s) 1112
Eco130I CCWWGG 3 cut(s) 553, 1026, 1092
Eco147I AGGCCT 1 cut(s) 920
Eco32I GATATC 1 cut(s) 840
Eco47I GGWCC 1 cut(s) 724
Eco57I CTGAAG 1 cut(s) 667
Eco81I CCTNAGG 1 cut(s) 631
EcoNI CCTNNNNNAGG 1 cut(s) 685
EcoO109I RGGNCCY 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 1112
EcoRV GATATC 1 cut(s) 840
EcoT14I CCWWGG 3 cut(s) 553, 1026, 1092
EgeI GGCGCC 1 cut(s) 190
EheI GGCGCC 1 cut(s) 190
ErhI CCWWGG 3 cut(s) 553, 1026, 1092
Esp3I CGTCTC 1 cut(s) 567
FaeI CATG 6 cut(s) 59, 244, 724, 879, 998, 1030
FaqI GGGAC 1 cut(s) 718
FatI CATG 6 cut(s) 55, 240, 720, 875, 994, 1026
FauNDI CATATG 1 cut(s) 623
FblI GTMKAC 1 cut(s) 320
Fnu4HI GCNGC 2 cut(s) 83, 881
FokI GGATG 3 cut(s) 217, 245, 344
Fsp4HI GCNGC 2 cut(s) 83, 881
FspBI CTAG 3 cut(s) 371, 515, 783
GlaI GCGC 3 cut(s) 86, 190, 483
GluI GCNGC 2 cut(s) 83, 881
GsaI CCCAGC 1 cut(s) 266
GsuI CTGGAG 1 cut(s) 591
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 4 cut(s) 49, 144, 449, 920
HapII CCGG 3 cut(s) 141, 645, 651
HhaI GCGC 3 cut(s) 87, 191, 484
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 6 cut(s) 59, 244, 724, 879, 998, 1030
Hin6I GCGC 3 cut(s) 85, 189, 482
HinP1I GCGC 3 cut(s) 85, 189, 482
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HindIII AAGCTT 1 cut(s) 1134
HinfI GANTC 2 cut(s) 111, 566
HpaII CCGG 3 cut(s) 141, 645, 651
HphI GGTGA 4 cut(s) 306, 517, 569, 821
Hpy166II GTNNAC 3 cut(s) 97, 321, 492
Hpy188I TCNGA 4 cut(s) 64, 686, 773, 1131
Hpy188III TCNNGA 1 cut(s) 570
Hpy8I GTNNAC 3 cut(s) 97, 321, 492
Hpy99I CGWCG 1 cut(s) 773
HpyAV CCTTC 7 cut(s) 8, 416, 460, 478, 592, 681, 691
HpyCH4III ACNGT 5 cut(s) 174, 214, 318, 809, 901
HpyCH4IV ACGT 2 cut(s) 707, 1119
HpyCH4V TGCA 5 cut(s) 155, 258, 437, 611, 627
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 455, 608
HpyF3I CTNAG 2 cut(s) 631, 672
HpySE526I ACGT 2 cut(s) 707, 1119
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 6 cut(s) 59, 244, 724, 879, 998, 1030
HspAI GCGC 3 cut(s) 85, 189, 482
KasI GGCGCC 1 cut(s) 188
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
Kzo9I GATC 4 cut(s) 68, 580, 984, 1081
LmnI GCTCC 1 cut(s) 647
Lsp1109I GCAGC 2 cut(s) 94, 892
LweI GCATC 5 cut(s) 267, 344, 366, 446, 647
MaeI CTAG 3 cut(s) 371, 515, 783
MaeII ACGT 2 cut(s) 707, 1119
MaeIII GTNAC 2 cut(s) 760, 856
MalI GATC 4 cut(s) 70, 582, 986, 1083
MbiI CCGCTC 1 cut(s) 716
MboI GATC 4 cut(s) 68, 580, 984, 1081
MboII GAAGA 9 cut(s) 180, 463, 572, 847, 915, 922, 979, 982, 1091
MfeI CAATTG 3 cut(s) 150, 443, 612
MluI ACGCGT 2 cut(s) 456, 1059
Mly113I GGCGCC 1 cut(s) 189
MlyI GAGTC 1 cut(s) 560
MmeI TCCRAC 3 cut(s) 87, 751, 874
MroNI GCCGGC 1 cut(s) 140
MseI TTAA 3 cut(s) 9, 1014, 1139
MspI CCGG 3 cut(s) 141, 645, 651
MspR9I CCNGG 1 cut(s) 1114
MunI CAATTG 3 cut(s) 150, 443, 612
MvaI CCWGG 1 cut(s) 1114
MvnI CGCG 3 cut(s) 458, 482, 1061
MwoI GCNNNNNNNGC 3 cut(s) 82, 455, 608
NaeI GCCGGC 1 cut(s) 142
NarI GGCGCC 1 cut(s) 189
NcoI CCATGG 1 cut(s) 1026
NdeI CATATG 1 cut(s) 623
NdeII GATC 4 cut(s) 68, 580, 984, 1081
NgoMIV GCCGGC 1 cut(s) 140
NheI GCTAGC 1 cut(s) 782
NlaIII CATG 6 cut(s) 59, 244, 724, 879, 998, 1030
NlaIV GGNNCC 2 cut(s) 190, 649
NmuCI GTSAC 1 cut(s) 856
NspI RCATGY 1 cut(s) 59
PceI AGGCCT 1 cut(s) 920
PciI ACATGT 1 cut(s) 55
PdiI GCCGGC 1 cut(s) 142
PfeI GAWTC 1 cut(s) 111
PfoI TCCNGGA 1 cut(s) 1112
PkrI GCNGC 2 cut(s) 84, 882
PleI GAGTC 1 cut(s) 560
PluTI GGCGCC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 560
PscI ACATGT 1 cut(s) 55
PsiI TTATAA 1 cut(s) 695
Psp1406I AACGTT 1 cut(s) 1119
Psp6I CCWGG 1 cut(s) 1112
PspFI CCCAGC 1 cut(s) 262
PspGI CCWGG 1 cut(s) 1112
PspN4I GGNNCC 2 cut(s) 190, 649
PspPI GGNCC 2 cut(s) 47, 724
RsaI GTAC 2 cut(s) 176, 219
RsaNI GTAC 2 cut(s) 175, 218
SaqAI TTAA 3 cut(s) 9, 1014, 1139
SatI GCNGC 2 cut(s) 83, 881
Sau3AI GATC 4 cut(s) 68, 580, 984, 1081
Sau96I GGNCC 2 cut(s) 47, 724
ScaI AGTACT 1 cut(s) 176
SchI GAGTC 1 cut(s) 560
ScrFI CCNGG 1 cut(s) 1114
SfaNI GCATC 5 cut(s) 267, 344, 366, 446, 647
SfoI GGCGCC 1 cut(s) 190
SinI GGWCC 1 cut(s) 724
SseBI AGGCCT 1 cut(s) 920
SsiI CCGC 5 cut(s) 80, 716, 765, 786, 1097
SspDI GGCGCC 1 cut(s) 188
SspMI CTAG 3 cut(s) 371, 515, 783
StuI AGGCCT 1 cut(s) 920
StyD4I CCNGG 1 cut(s) 1112
StyI CCWWGG 3 cut(s) 553, 1026, 1092
TaaI ACNGT 5 cut(s) 174, 214, 318, 809, 901
TaiI ACGT 2 cut(s) 710, 1122
TaqI TCGA 1 cut(s) 1077
TaqII GACCGA 1 cut(s) 81
TatI WGTACW 2 cut(s) 174, 217
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 3 cut(s) 9, 1014, 1139
Tru9I TTAA 3 cut(s) 9, 1014, 1139
TscAI CASTG 1 cut(s) 78
TseFI GTSAC 1 cut(s) 856
TseI GCWGC 2 cut(s) 82, 880
Tsp45I GTSAC 1 cut(s) 856
TspDTI ATGAA 3 cut(s) 610, 866, 1011
TspGWI ACGGA 1 cut(s) 379
TspRI CASTG 1 cut(s) 78
VpaK11BI GGWCC 1 cut(s) 724
XagI CCTNNNNNAGG 1 cut(s) 685
XapI RAATTY 2 cut(s) 327, 1108
XceI RCATGY 1 cut(s) 59
XcmI CCANNNNNNNNNTGG 1 cut(s) 949
XmiI GTMKAC 1 cut(s) 320
XspI CTAG 3 cut(s) 371, 515, 783
ZrmI AGTACT 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.