FvH4_3g06170

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002718
Physical Location & Seq
Reverse (-)
3546462 .. 3547998
1537 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06170.t1

Sequence Viewer

Length: 1197 bp
ATGGGAGAGGTAGAAGCTCAAGAACTGCAGCTGAGCATCAAGGTTCAAGAAGCAAATGAAGCAAAATCCCAACCAGATGTTCCTAATAACCAGCTGCCTCGGCGCAGAGGTTTCAACTGGTGGCTTAGAATATCGATTTACACAGTCTTTGTCCTTGCTGGCCAGGCAATTGCAACACTCTTGGGAAGACAGTACTATGAAAAAGGTGGAAGCAGCAACTGGCTTGCAACACTAGTACAGCTTTGTGGCTTTCCTATCCTCCTTCCCTTTTATTTCATTCCAGCATTAAACAAAAACTGTCCAACCACAAATAGTACTACATCATTCGAGTCAAAGCCACCGTCTACATTAGTCCTAGCATCAATTTATGCCTCTCTTGGCCTTCTTATAGCTGGACAATGCTACTTGTATTCAGTAGGATTATCTTACCTTCCTGTATCTACTTACTCCCTCATCTGCGCATCCCAACTCGCTTTCAATGCACTTTTCTCCTTCGTCCTCAACTCGCAGAAGTTCACTCCTTACATTGTCAACTCTCTTGTTGTCCTGACTATCTCATCCAGCCTCCTAGTGCTTCAAGGAGACGACTCTGCTGACTCGAGTAAAGTTTCCAGGCCAAAGTACATAATAGGCTTCTTCTGCACAGTTGGGGCATCAGCTGGATATGCATTACAGCTTTCCCTAACGCAGCTAATCTTCATAAAGGTTATAAAGAAAGAGACATTTAGAGTGATCATGGATATGATTGTATATCAATCAGTCTGTTCATCCGCTATTATCTTGGTGGGATTTTTTGCTAGTGGAGACTGGAAGGGTATAAAGAATGAGATGGAGGGGTTTGAGTTAGGGAAGGTGTCATATATTATGAATTTAACATGGACAGCTATAGTGTGGCAGCTCTTTTCCATAGGAATGGTGGGGTTAGTATTTGAGGCATCGGCCCTCTTCTCAAATTCAATAAGTGCTGTGGGTTTGCCTATTGTTCCAGTTCTAGCTGTGATCTTCTTCCATGACAAAATGGATGGGATAAAGGCAATGTCCATGGTTTTGGCTATTTGGGGATTCATTTCATATGTTTATCAGCACTACCTCGATGATCGTAAGTCCAAGAGTGAGAAGAAAGCTGCCAATGATGAAGTTTCTAAAGCTTTCTCTCTGGAAACACAAATGGTTGAACAATCAGCCTTGTATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

399

Amino Acids

43.96

Weight (kDa)

8.08

Isoelectric Point (pI)

32.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 43 - 368 6.7e-120 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
Acc16I TGCGCA 1 cut(s) 460
AccI GTMKAC 2 cut(s) 344, 1190
AciI CCGC 1 cut(s) 771
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 2 cut(s) 868, 952
AfaI GTAC 4 cut(s) 194, 237, 316, 623
AgsI TTSAA 6 cut(s) 47, 115, 478, 578, 957, 1175
AhlI ACTAGT 1 cut(s) 232
AjnI CCWGG 2 cut(s) 162, 611
AjuI GAANNNNNNNTTGG 2 cut(s) 63, 95
Alw26I GTCTC 3 cut(s) 576, 713, 798
AlwNI CAGNNNCTG 1 cut(s) 219
Ama87I CYCGRG 1 cut(s) 598
AoxI GGCC 4 cut(s) 160, 379, 614, 939
ApeKI GCWGC 6 cut(s) 28, 94, 213, 688, 895, 1124
ApoI RAATTY 2 cut(s) 868, 952
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AspLEI GCGC 2 cut(s) 105, 461
AspS9I GGNCC 1 cut(s) 940
AvaI CYCGRG 1 cut(s) 598
BalI TGGCCA 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 193
BbvI GCAGC 6 cut(s) 40, 81, 225, 700, 907, 1111
BccI CCATC 2 cut(s) 823, 1016
BciT130I CCWGG 2 cut(s) 164, 613
BclI TGATCA 1 cut(s) 732
BcoDI GTCTC 3 cut(s) 576, 713, 798
BcuI ACTAGT 1 cut(s) 232
BfaI CTAG 5 cut(s) 233, 356, 569, 798, 992
BfmI CTRYAG 2 cut(s) 26, 885
BisI GCNGC 6 cut(s) 29, 95, 214, 689, 896, 1125
BlpI GCTNAGC 1 cut(s) 32
BlsI GCNGC 6 cut(s) 30, 96, 215, 690, 897, 1126
BmcAI AGTACT 2 cut(s) 194, 316
Bme1390I CCNGG 2 cut(s) 164, 613
BmeT110I CYCGRG 1 cut(s) 598
BmgT120I GGNCC 1 cut(s) 940
BmrFI CCNGG 2 cut(s) 164, 613
BmsI GCATC 5 cut(s) 45, 368, 470, 662, 944
BpiI GAAGAC 1 cut(s) 193
Bpu1102I GCTNAGC 1 cut(s) 32
Bsa29I ATCGAT 1 cut(s) 134
BsaJI CCNNGG 2 cut(s) 98, 1041
Bse1I ACTGG 4 cut(s) 122, 224, 812, 986
Bse3DI GCAATG 1 cut(s) 1041
BseBI CCWGG 2 cut(s) 164, 613
BseCI ATCGAT 1 cut(s) 134
BseDI CCNNGG 2 cut(s) 98, 1041
BseGI GGATG 4 cut(s) 461, 557, 767, 1027
BseMI GCAATG 1 cut(s) 1041
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 4 cut(s) 122, 224, 812, 986
BseXI GCAGC 6 cut(s) 40, 81, 225, 700, 907, 1111
BsgI GTGCAG 1 cut(s) 625
BshFI GGCC 4 cut(s) 162, 381, 616, 941
BshVI ATCGAT 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 598
BsmAI GTCTC 3 cut(s) 576, 713, 798
BsmBI CGTCTC 1 cut(s) 576
BsnI GGCC 4 cut(s) 162, 381, 616, 941
BsoBI CYCGRG 1 cut(s) 598
Bsp143I GATC 3 cut(s) 732, 999, 1096
Bsp1720I GCTNAGC 1 cut(s) 32
Bsp19I CCATGG 1 cut(s) 1041
BspACI CCGC 1 cut(s) 771
BspANI GGCC 4 cut(s) 162, 381, 616, 941
BspCNI CTCAG 1 cut(s) 24
BspDI ATCGAT 1 cut(s) 134
BspMAI CTGCAG 1 cut(s) 30
BsrDI GCAATG 1 cut(s) 1041
BsrI ACTGG 4 cut(s) 122, 224, 812, 986
BssECI CCNNGG 2 cut(s) 98, 1041
BssMI GATC 3 cut(s) 732, 999, 1096
BssNAI GTATAC 1 cut(s) 1191
BssT1I CCWWGG 1 cut(s) 1041
Bst1107I GTATAC 1 cut(s) 1191
Bst2UI CCWGG 2 cut(s) 164, 613
Bst4CI ACNGT 5 cut(s) 145, 192, 299, 342, 646
Bst6I CTCTTC 1 cut(s) 950
BstC8I GCNNGC 2 cut(s) 160, 225
BstDEI CTNAG 2 cut(s) 32, 125
BstDSI CCRYGG 1 cut(s) 1041
BstF5I GGATG 4 cut(s) 461, 557, 767, 1027
BstHHI GCGC 2 cut(s) 105, 461
BstKTI GATC 3 cut(s) 735, 1002, 1099
BstMAI GTCTC 3 cut(s) 576, 713, 798
BstMBI GATC 3 cut(s) 732, 999, 1096
BstMWI GCNNNNNNNGC 6 cut(s) 59, 100, 164, 479, 639, 665
BstNI CCWGG 2 cut(s) 164, 613
BstSCI CCNGG 2 cut(s) 162, 611
BstSFI CTRYAG 2 cut(s) 26, 885
BstV1I GCAGC 6 cut(s) 40, 81, 225, 700, 907, 1111
BstV2I GAAGAC 1 cut(s) 193
BstXI CCANNNNNNTGG 2 cut(s) 913, 1048
BstZ17I GTATAC 1 cut(s) 1191
Bsu15I ATCGAT 1 cut(s) 134
BsuRI GGCC 4 cut(s) 162, 381, 616, 941
BsuTUI ATCGAT 1 cut(s) 134
BtgI CCRYGG 1 cut(s) 1041
BtsCI GGATG 4 cut(s) 461, 557, 767, 1027
Cac8I GCNNGC 2 cut(s) 160, 225
CaiI CAGNNNCTG 1 cut(s) 219
CfoI GCGC 2 cut(s) 105, 461
Cfr13I GGNCC 1 cut(s) 940
ClaI ATCGAT 1 cut(s) 134
Csp6I GTAC 4 cut(s) 193, 236, 315, 622
CviAII CATG 5 cut(s) 736, 876, 1010, 1042, 1194
CviQI GTAC 4 cut(s) 193, 236, 315, 622
DdeI CTNAG 2 cut(s) 32, 125
DpnI GATC 3 cut(s) 734, 1001, 1098
DpnII GATC 3 cut(s) 732, 999, 1096
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 950
EarI CTCTTC 1 cut(s) 950
Eco130I CCWWGG 1 cut(s) 1041
Eco88I CYCGRG 1 cut(s) 598
EcoRII CCWGG 2 cut(s) 162, 611
EcoT14I CCWWGG 1 cut(s) 1041
EcoT22I ATGCAT 1 cut(s) 670
ErhI CCWWGG 1 cut(s) 1041
Esp3I CGTCTC 1 cut(s) 576
FaeI CATG 5 cut(s) 739, 879, 1013, 1045, 1197
FatI CATG 5 cut(s) 735, 875, 1009, 1041, 1193
FauNDI CATATG 1 cut(s) 1072
FbaI TGATCA 1 cut(s) 732
FblI GTMKAC 2 cut(s) 344, 1190
Fnu4HI GCNGC 6 cut(s) 29, 95, 214, 689, 896, 1125
FokI GGATG 4 cut(s) 448, 544, 754, 1034
Fsp4HI GCNGC 6 cut(s) 29, 95, 214, 689, 896, 1125
FspBI CTAG 5 cut(s) 233, 356, 569, 798, 992
FspI TGCGCA 1 cut(s) 460
GlaI GCGC 2 cut(s) 104, 460
GluI GCNGC 6 cut(s) 29, 95, 214, 689, 896, 1125
HaeIII GGCC 4 cut(s) 162, 381, 616, 941
HhaI GCGC 2 cut(s) 105, 461
Hin1II CATG 5 cut(s) 739, 879, 1013, 1045, 1197
Hin6I GCGC 2 cut(s) 103, 459
HinP1I GCGC 2 cut(s) 103, 459
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HindIII AAGCTT 1 cut(s) 1146
HinfI GANTC 4 cut(s) 329, 587, 596, 1062
Hpy166II GTNNAC 4 cut(s) 345, 516, 532, 1191
Hpy188III TCNNGA 4 cut(s) 20, 47, 547, 1157
Hpy8I GTNNAC 4 cut(s) 345, 516, 532, 1191
HpyAV CCTTC 6 cut(s) 272, 392, 440, 502, 805, 844
HpyCH4III ACNGT 5 cut(s) 145, 192, 299, 342, 646
HpyCH4V TGCA 6 cut(s) 28, 173, 227, 482, 642, 668
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 100, 164, 479, 639, 665
HpyF3I CTNAG 2 cut(s) 32, 125
Hsp92II CATG 5 cut(s) 739, 879, 1013, 1045, 1197
HspAI GCGC 2 cut(s) 103, 459
Ksp22I TGATCA 1 cut(s) 732
Kzo9I GATC 3 cut(s) 732, 999, 1096
Lsp1109I GCAGC 6 cut(s) 40, 81, 225, 700, 907, 1111
LweI GCATC 5 cut(s) 45, 368, 470, 662, 944
MaeI CTAG 5 cut(s) 233, 356, 569, 798, 992
MalI GATC 3 cut(s) 734, 1001, 1098
MboI GATC 3 cut(s) 732, 999, 1096
MboII GAAGA 7 cut(s) 198, 628, 688, 937, 994, 997, 1129
MfeI CAATTG 1 cut(s) 168
MlsI TGGCCA 1 cut(s) 162
MluCI AATT 4 cut(s) 168, 363, 868, 952
MluNI TGGCCA 1 cut(s) 162
MlyI GAGTC 3 cut(s) 338, 581, 590
MmeI TCCRAC 1 cut(s) 326
Mox20I TGGCCA 1 cut(s) 162
Mph1103I ATGCAT 1 cut(s) 670
MscI TGGCCA 1 cut(s) 162
MseI TTAA 2 cut(s) 287, 872
MslI CAYNNNNRTG 2 cut(s) 740, 911
Msp20I TGGCCA 1 cut(s) 162
MspA1I CMGCKG 3 cut(s) 31, 94, 659
MspR9I CCNGG 2 cut(s) 164, 613
MunI CAATTG 1 cut(s) 168
MvaI CCWGG 2 cut(s) 164, 613
MwoI GCNNNNNNNGC 6 cut(s) 59, 100, 164, 479, 639, 665
NcoI CCATGG 1 cut(s) 1041
NdeI CATATG 1 cut(s) 1072
NdeII GATC 3 cut(s) 732, 999, 1096
NlaIII CATG 5 cut(s) 739, 879, 1013, 1045, 1197
NmeAIII GCCGAG 1 cut(s) 79
NsbI TGCGCA 1 cut(s) 460
NsiI ATGCAT 1 cut(s) 670
PaeR7I CTCGAG 1 cut(s) 598
PfeI GAWTC 1 cut(s) 1062
PkrI GCNGC 6 cut(s) 30, 96, 215, 690, 897, 1126
PleI GAGTC 3 cut(s) 337, 581, 590
PpsI GAGTC 3 cut(s) 337, 581, 590
PsiI TTATAA 1 cut(s) 710
Psp6I CCWGG 2 cut(s) 162, 611
PspGI CCWGG 2 cut(s) 162, 611
PspPI GGNCC 1 cut(s) 940
PspXI VCTCGAGB 1 cut(s) 598
PstI CTGCAG 1 cut(s) 30
PstNI CAGNNNCTG 1 cut(s) 219
PvuII CAGCTG 3 cut(s) 31, 94, 659
RsaI GTAC 4 cut(s) 194, 237, 316, 623
RsaNI GTAC 4 cut(s) 193, 236, 315, 622
RseI CAYNNNNRTG 2 cut(s) 740, 911
SaqAI TTAA 2 cut(s) 287, 872
SatI GCNGC 6 cut(s) 29, 95, 214, 689, 896, 1125
Sau3AI GATC 3 cut(s) 732, 999, 1096
Sau96I GGNCC 1 cut(s) 940
ScaI AGTACT 2 cut(s) 194, 316
SchI GAGTC 3 cut(s) 338, 581, 590
ScrFI CCNGG 2 cut(s) 164, 613
SfaNI GCATC 5 cut(s) 45, 368, 470, 662, 944
SfcI CTRYAG 2 cut(s) 26, 885
Sfr274I CTCGAG 1 cut(s) 598
SlaI CTCGAG 1 cut(s) 598
SmiMI CAYNNNNRTG 2 cut(s) 740, 911
SmlI CTYRAG 2 cut(s) 18, 598
SmoI CTYRAG 2 cut(s) 18, 598
SpeI ACTAGT 1 cut(s) 232
Sse9I AATT 4 cut(s) 168, 363, 868, 952
SsiI CCGC 1 cut(s) 771
SspMI CTAG 5 cut(s) 233, 356, 569, 798, 992
StyD4I CCNGG 2 cut(s) 162, 611
StyI CCWWGG 1 cut(s) 1041
TaaI ACNGT 5 cut(s) 145, 192, 299, 342, 646
TaqI TCGA 4 cut(s) 134, 327, 599, 1092
TasI AATT 4 cut(s) 168, 363, 868, 952
TatI WGTACW 4 cut(s) 192, 235, 314, 621
TfiI GAWTC 1 cut(s) 1062
Tru1I TTAA 2 cut(s) 287, 872
Tru9I TTAA 2 cut(s) 287, 872
TseI GCWGC 6 cut(s) 28, 94, 213, 688, 895, 1124
TspDTI ATGAA 9 cut(s) 72, 213, 265, 688, 756, 881, 1054, 1059, 1149
XapI RAATTY 2 cut(s) 868, 952
XcmI CCANNNNNNNNNTGG 1 cut(s) 913
XhoI CTCGAG 1 cut(s) 598
XmiI GTMKAC 2 cut(s) 344, 1190
XspI CTAG 5 cut(s) 233, 356, 569, 798, 992
ZrmI AGTACT 2 cut(s) 194, 316
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.