AT4G18205

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002718
Physical Location & Seq
Forward (+)
10073711 .. 10075370
1660 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18205.1

Sequence Viewer

Length: 1134 bp
ATGGGCATATCTCAAGTACACTATTGCAATGGTGACCAGAACTTAGAAGCAAACCTTCTAGATCATGAGGAAACTGAATCATTTTCAGTACCTCAAACGAAGAACTGTAAGAGGTGGCTCCGTGTCTCCATATACGCAATCTTTGTCATCTTCTGCCAACCACTTGCTACAGTTCTGGGTAGACTGTACTATGAAAATGGAGGGAAAAGCACATATGTGGTAACACTTCTTCAACTCATTGGCTTCCCTGTACTGATTCTCTTCCGCTTCTTTTCTCGAATCAGGCAACCCAAATCAACAGATACAAATTTCAGTCAGTCCCCTTCCTTCACCACCCTTGCATCGGTTTACTTGTGCACTGGACTGCTAGTGTCTGCTTATGCTTATTTGTCTGCAGTTGGGTTGCTTTACTTACCAGTCTCTACTTTCTCCCTCATCTTGGCCTCACAGTTGGCCTTCACTGCCTTTTTCTCATATTTCCTTAACTCGCAAAAGTTCACTCCTTTGATAGTCAATTCTTTGTTTCTCCTTACGGTTTCCTCTGCCCTCCTCGTGGTCAACACTGATTCAGAAAACACAACAAATGTATCTAGAGTACAATATGTGATTGGGTTCATCTGTACCATTGGTGCTTCCGCTGGGATTGGATTGGTACTATCTCTGATACAACTGCTCTTCAGGAAAGTTTTCACGAAGCATACATCCTCAGCAGTCCTGGACTTGGCCAATTACCAGTCTCTAGTTGCAACTTGTGTGGTACTCATAGGACTGTTTGCAAGTGGAGAGTGGAGAACTCTGCCAAGTGAGATGAGAAACTACAAACTGGGGAAAGTGTCATATATCTTGACTTTGGCCTCAGCAGCTATTTTCTGGCAAGTATACACTGTTGGTTGTGTGGGATTAATCTTCGAGTCTTCTTCTGTGTTCTCCAATTCCATAACTGCTGTGGGACTGCCTATAGTTCCAGTTGTAGCAGTGATAGTTTTCCATGATAAGATGGATGCATCAAAGATTTTCTCCATCATTTTAGCTATCTGGGGCTTCCTTTCATTTGTCTATCAGCACTACCTCGACGAAAAGAAGTTGAAGACTTGCCAGACAAAACCTGTTGAGGAAGAAACACAAACTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

377

Amino Acids

41.79

Weight (kDa)

8.52

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 41 - 361 4.3e-119 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 181, 879
AciI CCGC 2 cut(s) 265, 636
AcoI YGGCCR 1 cut(s) 723
AcsI RAATTY 1 cut(s) 307
AcuI CTGAAG 1 cut(s) 661
AfaI GTAC 8 cut(s) 18, 90, 188, 252, 597, 622, 654, 759
AfiI CCNNNNNNNGG 4 cut(s) 343, 439, 553, 721
AgsI TTSAA 2 cut(s) 233, 1087
AjnI CCWGG 1 cut(s) 714
AleI CACNNNNGTG 1 cut(s) 215
AluBI AGCT 2 cut(s) 863, 1031
AluI AGCT 2 cut(s) 863, 1031
Alw21I GWGCWC 1 cut(s) 359
Alw26I GTCTC 3 cut(s) 130, 424, 741
Alw44I GTGCAC 1 cut(s) 355
AoxI GGCC 4 cut(s) 441, 453, 723, 852
ApaLI GTGCAC 1 cut(s) 355
ApeKI GCWGC 1 cut(s) 860
ApoI RAATTY 1 cut(s) 307
AseI ATTAAT 1 cut(s) 902
Asp700I GAANNNNTTC 1 cut(s) 686
AsuHPI GGTGA 2 cut(s) 44, 322
BaeGI GKGCMC 1 cut(s) 359
BaeI ACNNNNGTAYC 2 cut(s) 570, 603
BalI TGGCCA 1 cut(s) 725
BarI GAAGNNNNNNTAC 2 cut(s) 213, 245
BauI CACGAG 1 cut(s) 551
BbsI GAAGAC 2 cut(s) 906, 1094
Bbv12I GWGCWC 1 cut(s) 359
BbvCI CCTCAGC 2 cut(s) 706, 856
BbvI GCAGC 1 cut(s) 872
BccI CCATC 2 cut(s) 991, 1028
BciT130I CCWGG 1 cut(s) 716
BcoDI GTCTC 3 cut(s) 130, 424, 741
BfaI CTAG 4 cut(s) 59, 368, 591, 740
BfmI CTRYAG 3 cut(s) 168, 393, 957
BisI GCNGC 1 cut(s) 861
BlsI GCNGC 1 cut(s) 862
Bme1390I CCNGG 1 cut(s) 716
BmiI GGNNCC 1 cut(s) 119
BmrFI CCNGG 1 cut(s) 716
BmrI ACTGGG 1 cut(s) 833
BmsI GCATC 3 cut(s) 350, 991, 1013
BmuI ACTGGG 1 cut(s) 833
BpiI GAAGAC 2 cut(s) 906, 1094
Bpu10I CCTNAGC 2 cut(s) 706, 856
Bsc4I CCNNNNNNNGG 4 cut(s) 343, 439, 553, 721
Bse1I ACTGG 5 cut(s) 364, 416, 733, 828, 965
Bse3DI GCAATG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 716
BseGI GGATG 2 cut(s) 701, 1006
BseLI CCNNNNNNNGG 4 cut(s) 343, 439, 553, 721
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 2 cut(s) 720, 870
BseNI ACTGG 5 cut(s) 364, 416, 733, 828, 965
BseRI GAGGAG 1 cut(s) 539
BseSI GKGCMC 1 cut(s) 359
BseXI GCAGC 1 cut(s) 872
BseYI CCCAGC 1 cut(s) 638
BshFI GGCC 4 cut(s) 443, 455, 725, 854
BsiHKAI GWGCWC 1 cut(s) 359
BslFI GGGAC 2 cut(s) 304, 963
BslI CCNNNNNNNGG 4 cut(s) 343, 439, 553, 721
BsmAI GTCTC 3 cut(s) 130, 424, 741
BsmFI GGGAC 2 cut(s) 304, 963
BsnI GGCC 4 cut(s) 443, 455, 725, 854
Bsp1286I GDGCHC 1 cut(s) 359
Bsp143I GATC 1 cut(s) 61
BspACI CCGC 2 cut(s) 265, 636
BspANI GGCC 4 cut(s) 443, 455, 725, 854
BspCNI CTCAG 2 cut(s) 719, 869
BspHI TCATGA 1 cut(s) 64
BspLI GGNNCC 1 cut(s) 119
BspMAI CTGCAG 1 cut(s) 397
BspQI GCTCTTC 1 cut(s) 680
BsrDI GCAATG 1 cut(s) 34
BsrI ACTGG 5 cut(s) 364, 416, 733, 828, 965
BssMI GATC 1 cut(s) 61
BssNAI GTATAC 1 cut(s) 880
BssSI CACGAG 1 cut(s) 551
Bst1107I GTATAC 1 cut(s) 880
Bst2BI CACGAG 1 cut(s) 551
Bst2UI CCWGG 1 cut(s) 716
Bst4CI ACNGT 7 cut(s) 107, 172, 186, 450, 535, 771, 886
Bst6I CTCTTC 2 cut(s) 266, 680
BstDEI CTNAG 3 cut(s) 43, 706, 856
BstEII GGTNACC 1 cut(s) 32
BstF5I GGATG 2 cut(s) 701, 1006
BstKTI GATC 1 cut(s) 64
BstMAI GTCTC 3 cut(s) 130, 424, 741
BstMBI GATC 1 cut(s) 61
BstMWI GCNNNNNNNGC 2 cut(s) 461, 860
BstNI CCWGG 1 cut(s) 716
BstPI GGTNACC 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 714
BstSFI CTRYAG 3 cut(s) 168, 393, 957
BstSLI GKGCMC 1 cut(s) 359
BstV1I GCAGC 1 cut(s) 872
BstV2I GAAGAC 2 cut(s) 906, 1094
BstZ17I GTATAC 1 cut(s) 880
BsuRI GGCC 4 cut(s) 443, 455, 725, 854
BtsCI GGATG 2 cut(s) 701, 1006
BtsI GCAGTG 2 cut(s) 459, 981
BtsIMutI CAGTG 5 cut(s) 357, 459, 561, 882, 981
CciI TCATGA 1 cut(s) 64
Csp6I GTAC 8 cut(s) 17, 89, 187, 251, 596, 621, 653, 758
CspCI CAANNNNNGTGG 2 cut(s) 735, 770
CviAII CATG 2 cut(s) 65, 989
CviJI RGCY 9 cut(s) 118, 243, 443, 455, 725, 854, 863, 1031, 1041
CviKI_1 RGCY 9 cut(s) 118, 243, 443, 455, 725, 854, 863, 1031, 1041
CviQI GTAC 8 cut(s) 17, 89, 187, 251, 596, 621, 653, 758
DdeI CTNAG 3 cut(s) 43, 706, 856
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 723
Eam1104I CTCTTC 2 cut(s) 266, 680
EarI CTCTTC 2 cut(s) 266, 680
Eco57I CTGAAG 1 cut(s) 661
Eco91I GGTNACC 1 cut(s) 32
EcoO65I GGTNACC 1 cut(s) 32
EcoRII CCWGG 1 cut(s) 714
EcoT22I ATGCAT 1 cut(s) 1006
FaeI CATG 2 cut(s) 68, 992
FalI AAGNNNNNCTT 2 cut(s) 39, 71
FaqI GGGAC 2 cut(s) 304, 963
FatI CATG 2 cut(s) 64, 988
FauNDI CATATG 1 cut(s) 214
FblI GTMKAC 2 cut(s) 181, 879
Fnu4HI GCNGC 1 cut(s) 861
FokI GGATG 2 cut(s) 688, 1013
Fsp4HI GCNGC 1 cut(s) 861
FspBI CTAG 4 cut(s) 59, 368, 591, 740
GluI GCNGC 1 cut(s) 861
GsaI CCCAGC 1 cut(s) 642
HaeIII GGCC 4 cut(s) 443, 455, 725, 854
Hin1II CATG 2 cut(s) 68, 992
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 5 cut(s) 77, 256, 279, 566, 911
HphI GGTGA 2 cut(s) 44, 322
Hpy166II GTNNAC 7 cut(s) 19, 182, 349, 357, 498, 559, 880
Hpy188I TCNGA 2 cut(s) 571, 663
Hpy188III TCNNGA 7 cut(s) 59, 65, 276, 591, 679, 691, 844
Hpy8I GTNNAC 7 cut(s) 19, 182, 349, 357, 498, 559, 880
Hpy99I CGWCG 1 cut(s) 1076
HpyAV CCTTC 4 cut(s) 65, 333, 337, 466
HpyCH4III ACNGT 7 cut(s) 107, 172, 186, 450, 535, 771, 886
HpyCH4V TGCA 7 cut(s) 27, 341, 357, 395, 746, 776, 1004
HpyF10VI GCNNNNNNNGC 2 cut(s) 461, 860
HpyF3I CTNAG 3 cut(s) 43, 706, 856
Hsp92II CATG 2 cut(s) 68, 992
Kzo9I GATC 1 cut(s) 61
LguI GCTCTTC 1 cut(s) 680
LmnI GCTCC 1 cut(s) 123
Lsp1109I GCAGC 1 cut(s) 872
LweI GCATC 3 cut(s) 350, 991, 1013
MaeI CTAG 4 cut(s) 59, 368, 591, 740
MaeIII GTNAC 2 cut(s) 32, 220
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MhlI GDGCHC 1 cut(s) 359
MlsI TGGCCA 1 cut(s) 725
MluCI AATT 4 cut(s) 307, 514, 727, 931
MluNI TGGCCA 1 cut(s) 725
MlyI GAGTC 1 cut(s) 920
Mox20I TGGCCA 1 cut(s) 725
Mph1103I ATGCAT 1 cut(s) 1006
MroXI GAANNNNTTC 1 cut(s) 686
MscI TGGCCA 1 cut(s) 725
MseI TTAA 2 cut(s) 483, 902
MslI CAYNNNNRTG 1 cut(s) 215
Msp20I TGGCCA 1 cut(s) 725
MspA1I CMGCKG 1 cut(s) 638
MspR9I CCNGG 1 cut(s) 716
MvaI CCWGG 1 cut(s) 716
MwoI GCNNNNNNNGC 2 cut(s) 461, 860
NdeI CATATG 1 cut(s) 214
NdeII GATC 1 cut(s) 61
NlaIII CATG 2 cut(s) 68, 992
NlaIV GGNNCC 1 cut(s) 119
NmuCI GTSAC 1 cut(s) 32
NsiI ATGCAT 1 cut(s) 1006
OliI CACNNNNGTG 1 cut(s) 215
PagI TCATGA 1 cut(s) 64
PciSI GCTCTTC 1 cut(s) 680
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 4 cut(s) 77, 256, 279, 566
PfoI TCCNGGA 1 cut(s) 714
PkrI GCNGC 1 cut(s) 862
PleI GAGTC 1 cut(s) 919
PpsI GAGTC 1 cut(s) 919
PshBI ATTAAT 1 cut(s) 902
Psp6I CCWGG 1 cut(s) 714
PspEI GGTNACC 1 cut(s) 32
PspFI CCCAGC 1 cut(s) 638
PspGI CCWGG 1 cut(s) 714
PspN4I GGNNCC 1 cut(s) 119
PstI CTGCAG 1 cut(s) 397
RsaI GTAC 8 cut(s) 18, 90, 188, 252, 597, 622, 654, 759
RsaNI GTAC 8 cut(s) 17, 89, 187, 251, 596, 621, 653, 758
RseI CAYNNNNRTG 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 680
SaqAI TTAA 2 cut(s) 483, 902
SatI GCNGC 1 cut(s) 861
Sau3AI GATC 1 cut(s) 61
SchI GAGTC 1 cut(s) 920
ScrFI CCNGG 1 cut(s) 716
SduI GDGCHC 1 cut(s) 359
SetI ASST 7 cut(s) 57, 94, 116, 865, 1033, 1071, 1108
SfaNI GCATC 3 cut(s) 350, 991, 1013
SfcI CTRYAG 3 cut(s) 168, 393, 957
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
Sse9I AATT 4 cut(s) 307, 514, 727, 931
SsiI CCGC 2 cut(s) 265, 636
SspMI CTAG 4 cut(s) 59, 368, 591, 740
StyD4I CCNGG 1 cut(s) 714
TaaI ACNGT 7 cut(s) 107, 172, 186, 450, 535, 771, 886
TaqI TCGA 3 cut(s) 277, 909, 1071
TasI AATT 4 cut(s) 307, 514, 727, 931
TatI WGTACW 4 cut(s) 16, 186, 250, 595
TfiI GAWTC 4 cut(s) 77, 256, 279, 566
Tru1I TTAA 2 cut(s) 483, 902
Tru9I TTAA 2 cut(s) 483, 902
TscAI CASTG 5 cut(s) 364, 466, 568, 889, 981
TseFI GTSAC 1 cut(s) 32
TseI GCWGC 1 cut(s) 860
Tsp45I GTSAC 1 cut(s) 32
TspDTI ATGAA 3 cut(s) 207, 604, 1038
TspGWI ACGGA 1 cut(s) 110
TspRI CASTG 5 cut(s) 364, 466, 568, 889, 981
VneI GTGCAC 1 cut(s) 355
VspI ATTAAT 1 cut(s) 902
XapI RAATTY 1 cut(s) 307
XbaI TCTAGA 2 cut(s) 58, 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XmiI GTMKAC 2 cut(s) 181, 879
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 4 cut(s) 59, 368, 591, 740
Zsp2I ATGCAT 1 cut(s) 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.