Rroxscaffold_1G00063430

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002718
Physical Location & Seq
Reverse (-)
85303730 .. 85306790
3061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00063430.1

Sequence Viewer

Length: 1176 bp
ATGGGAGAAGTGGAAGCTCGAGAACTGCAGCTGAGCATCATGGTTCAAGCAGAAGCAAATGAAGTAAACTCCCTACCATATGTTCCTAACCAGCTGTCTCGGCGCAGAGGTTTTAACTGGTGGCTTAGAATGTCGATTTACACAGTGTTTGTCCTCGCTGGCCAGGCAATTGCAACACTTTTGGGAAGACAGTACTATGAAAAAGGTGGTAAAAGCAACTGGCTTGCTACACTAGTACAACTTTGTGGCTTTCCTATCCTGCTTCCCTTTTATTTCGTTCCAGCATTGAACAAAAACTGTCCAACCACAAATAGTACTATAGCATTTGTGTCAAAGCCACCGTCTACATTAGTCCTTGCATCAATTTATGCCTCTCTTGGCCTCCTTATAGCAGCAGACTGCTACTTGTATTCAGTAGGACTATCTTACCTTCCTGTATCTACTTACTCCCTCATTTGCGCATCCCAACTCGCCTTCAATGCGCTTTTCTCTTTCTTCCTCAACTCGCAGAAGTTTACTCCTTACATTGTCAACTCTCTTGTTGTACTGACTATCTCATCCAGCCTCCTAGTGCTTCAAGGAGACGACTCTGCTGACTCGAGTAAAGTTTCCAGGTCAAAGTACATAATAGGCTTCATCTGCACCGTTGGGGCATCGGCTGGATATGCATTGACACTTTCTCTGACGCAGCTAGCCTTCATAAAGGTTATAAAGAAAGAAACATTTAGAGTGATCATGGACATGATAGTGTTTCAATCAGTCTTTTCAACTGCTATTATATTGGTGGGATTTTTTGCTAGTGGAGACTGGAAGGGTATAAAGGATGAGATGGAGGGATTTGAGTTAGGGAAGGTTTCCTATATTATGAATTTAACATGGACAGCTATAATGTGGCAGCTCTTTGCCATTGGAATAGTGGGGTTGGTATTTGAGGCGTCGTCCCTCTTCTCAAATTCTATTAGTGCTGTGGGATTGCCTATTGTTCCAGTTCTAGCTGTGATCTTCTTCCATGACAAAATGGATGGGATAAAGGTAATGTCCATGGTTTTGGCTATCTGGGGATTTATTTCATATGTTTATCAGCACTACCTCGATGATCGTAAGTTCAAGAGTGAGAAGAAAGCTGCCAATGATGAAGTTTCTAAAGCTTTTGCACAGGAAAACATTAATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

391

Amino Acids

43.22

Weight (kDa)

8.43

Isoelectric Point (pI)

31.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 43 - 368 2.1e-121 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
Acc16I TGCGCA 1 cut(s) 460
AccI GTMKAC 1 cut(s) 344
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 2 cut(s) 868, 952
AcyI GRCGYC 1 cut(s) 935
AfaI GTAC 5 cut(s) 194, 237, 316, 546, 623
AgsI TTSAA 7 cut(s) 47, 289, 478, 578, 755, 768, 1108
AhlI ACTAGT 1 cut(s) 232
AjnI CCWGG 2 cut(s) 162, 611
AluBI AGCT 9 cut(s) 17, 31, 94, 691, 884, 898, 995, 1124, 1148
AluI AGCT 9 cut(s) 17, 31, 94, 691, 884, 898, 995, 1124, 1148
Alw26I GTCTC 3 cut(s) 102, 576, 798
Ama87I CYCGRG 2 cut(s) 18, 598
AoxI GGCC 2 cut(s) 160, 379
ApeKI GCWGC 5 cut(s) 28, 392, 688, 895, 1124
ApoI RAATTY 2 cut(s) 868, 952
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AseI ATTAAT 1 cut(s) 1167
AspLEI GCGC 3 cut(s) 105, 461, 484
AsuNHI GCTAGC 1 cut(s) 691
AvaI CYCGRG 2 cut(s) 18, 598
BalI TGGCCA 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 193
BbvI GCAGC 5 cut(s) 40, 404, 700, 907, 1111
BccI CCATC 2 cut(s) 823, 1016
BciT130I CCWGG 2 cut(s) 164, 613
BclI TGATCA 1 cut(s) 732
BcoDI GTCTC 3 cut(s) 102, 576, 798
BcuI ACTAGT 1 cut(s) 232
BfaI CTAG 5 cut(s) 233, 569, 692, 798, 992
BfmI CTRYAG 2 cut(s) 26, 318
BisI GCNGC 5 cut(s) 29, 393, 689, 896, 1125
BlpI GCTNAGC 1 cut(s) 32
BlsI GCNGC 5 cut(s) 30, 394, 690, 897, 1126
BmcAI AGTACT 2 cut(s) 194, 316
Bme1390I CCNGG 2 cut(s) 164, 613
BmeT110I CYCGRG 2 cut(s) 18, 598
BmrFI CCNGG 2 cut(s) 164, 613
BmsI GCATC 4 cut(s) 45, 368, 470, 662
BmtI GCTAGC 1 cut(s) 695
BpiI GAAGAC 1 cut(s) 193
Bpu1102I GCTNAGC 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 935
BsaJI CCNNGG 1 cut(s) 1041
Bse1I ACTGG 4 cut(s) 122, 224, 812, 986
BseBI CCWGG 2 cut(s) 164, 613
BseDI CCNNGG 1 cut(s) 1041
BseGI GGATG 4 cut(s) 461, 557, 829, 1027
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 4 cut(s) 122, 224, 812, 986
BseXI GCAGC 5 cut(s) 40, 404, 700, 907, 1111
BsgI GTGCAG 1 cut(s) 625
BshFI GGCC 2 cut(s) 162, 381
BsiHKCI CYCGRG 2 cut(s) 18, 598
BslFI GGGAC 1 cut(s) 925
BsmAI GTCTC 3 cut(s) 102, 576, 798
BsmBI CGTCTC 1 cut(s) 576
BsmFI GGGAC 1 cut(s) 925
BsnI GGCC 2 cut(s) 162, 381
BsoBI CYCGRG 2 cut(s) 18, 598
Bsp143I GATC 3 cut(s) 732, 999, 1096
Bsp1720I GCTNAGC 1 cut(s) 32
Bsp19I CCATGG 1 cut(s) 1041
BspANI GGCC 2 cut(s) 162, 381
BspCNI CTCAG 1 cut(s) 24
BspMAI CTGCAG 1 cut(s) 30
BspOI GCTAGC 1 cut(s) 695
BsrI ACTGG 4 cut(s) 122, 224, 812, 986
BssECI CCNNGG 1 cut(s) 1041
BssMI GATC 3 cut(s) 732, 999, 1096
BssNI GRCGYC 1 cut(s) 935
BssT1I CCWWGG 1 cut(s) 1041
Bst2UI CCWGG 2 cut(s) 164, 613
Bst4CI ACNGT 5 cut(s) 145, 192, 299, 342, 646
Bst6I CTCTTC 1 cut(s) 950
BstACI GRCGYC 1 cut(s) 935
BstC8I GCNNGC 3 cut(s) 160, 225, 693
BstDEI CTNAG 2 cut(s) 32, 125
BstDSI CCRYGG 1 cut(s) 1041
BstF5I GGATG 4 cut(s) 461, 557, 829, 1027
BstHHI GCGC 3 cut(s) 105, 461, 484
BstKTI GATC 3 cut(s) 735, 1002, 1099
BstMAI GTCTC 3 cut(s) 102, 576, 798
BstMBI GATC 3 cut(s) 732, 999, 1096
BstMWI GCNNNNNNNGC 5 cut(s) 100, 164, 479, 639, 665
BstNI CCWGG 2 cut(s) 164, 613
BstSCI CCNGG 2 cut(s) 162, 611
BstSFI CTRYAG 2 cut(s) 26, 318
BstV1I GCAGC 5 cut(s) 40, 404, 700, 907, 1111
BstV2I GAAGAC 1 cut(s) 193
BstXI CCANNNNNNTGG 1 cut(s) 1048
BsuRI GGCC 2 cut(s) 162, 381
BtgI CCRYGG 1 cut(s) 1041
BtsCI GGATG 4 cut(s) 461, 557, 829, 1027
BtsIMutI CAGTG 1 cut(s) 150
Cac8I GCNNGC 3 cut(s) 160, 225, 693
CfoI GCGC 3 cut(s) 105, 461, 484
CseI GACGC 2 cut(s) 694, 924
Csp6I GTAC 5 cut(s) 193, 236, 315, 545, 622
CviAII CATG 6 cut(s) 40, 736, 742, 876, 1010, 1042
CviQI GTAC 5 cut(s) 193, 236, 315, 545, 622
DdeI CTNAG 2 cut(s) 32, 125
DpnI GATC 3 cut(s) 734, 1001, 1098
DpnII GATC 3 cut(s) 732, 999, 1096
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 950
EarI CTCTTC 1 cut(s) 950
Eco130I CCWWGG 1 cut(s) 1041
Eco88I CYCGRG 2 cut(s) 18, 598
EcoRII CCWGG 2 cut(s) 162, 611
EcoT14I CCWWGG 1 cut(s) 1041
EcoT22I ATGCAT 1 cut(s) 670
ErhI CCWWGG 1 cut(s) 1041
Esp3I CGTCTC 1 cut(s) 576
FaeI CATG 6 cut(s) 43, 739, 745, 879, 1013, 1045
FaqI GGGAC 1 cut(s) 925
FatI CATG 6 cut(s) 39, 735, 741, 875, 1009, 1041
FauNDI CATATG 2 cut(s) 79, 1072
FbaI TGATCA 1 cut(s) 732
FblI GTMKAC 1 cut(s) 344
Fnu4HI GCNGC 5 cut(s) 29, 393, 689, 896, 1125
FokI GGATG 4 cut(s) 448, 544, 836, 1034
Fsp4HI GCNGC 5 cut(s) 29, 393, 689, 896, 1125
FspBI CTAG 5 cut(s) 233, 569, 692, 798, 992
FspI TGCGCA 1 cut(s) 460
GlaI GCGC 3 cut(s) 104, 460, 483
GluI GCNGC 5 cut(s) 29, 393, 689, 896, 1125
HaeIII GGCC 2 cut(s) 162, 381
HgaI GACGC 2 cut(s) 694, 924
HhaI GCGC 3 cut(s) 105, 461, 484
Hin1I GRCGYC 1 cut(s) 935
Hin1II CATG 6 cut(s) 43, 739, 745, 879, 1013, 1045
Hin6I GCGC 3 cut(s) 103, 459, 482
HinP1I GCGC 3 cut(s) 103, 459, 482
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HindIII AAGCTT 1 cut(s) 1146
HinfI GANTC 2 cut(s) 587, 596
Hpy166II GTNNAC 4 cut(s) 67, 345, 516, 532
Hpy188I TCNGA 1 cut(s) 684
Hpy188III TCNNGA 2 cut(s) 20, 1108
Hpy8I GTNNAC 4 cut(s) 67, 345, 516, 532
Hpy99I CGWCG 1 cut(s) 940
HpyAV CCTTC 5 cut(s) 440, 484, 706, 805, 844
HpyCH4III ACNGT 5 cut(s) 145, 192, 299, 342, 646
HpyCH4V TGCA 6 cut(s) 28, 173, 359, 642, 668, 1154
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 164, 479, 639, 665
HpyF3I CTNAG 2 cut(s) 32, 125
Hsp92I GRCGYC 1 cut(s) 935
Hsp92II CATG 6 cut(s) 43, 739, 745, 879, 1013, 1045
HspAI GCGC 3 cut(s) 103, 459, 482
Ksp22I TGATCA 1 cut(s) 732
Kzo9I GATC 3 cut(s) 732, 999, 1096
Lsp1109I GCAGC 5 cut(s) 40, 404, 700, 907, 1111
LweI GCATC 4 cut(s) 45, 368, 470, 662
MaeI CTAG 5 cut(s) 233, 569, 692, 798, 992
MalI GATC 3 cut(s) 734, 1001, 1098
MboI GATC 3 cut(s) 732, 999, 1096
MboII GAAGA 6 cut(s) 198, 487, 937, 994, 997, 1129
MfeI CAATTG 1 cut(s) 168
MlsI TGGCCA 1 cut(s) 162
MluCI AATT 4 cut(s) 168, 363, 868, 952
MluNI TGGCCA 1 cut(s) 162
MlyI GAGTC 2 cut(s) 581, 590
MmeI TCCRAC 1 cut(s) 326
Mox20I TGGCCA 1 cut(s) 162
Mph1103I ATGCAT 1 cut(s) 670
MscI TGGCCA 1 cut(s) 162
MseI TTAA 3 cut(s) 114, 872, 1167
MslI CAYNNNNRTG 2 cut(s) 740, 746
Msp20I TGGCCA 1 cut(s) 162
MspA1I CMGCKG 2 cut(s) 31, 94
MspR9I CCNGG 2 cut(s) 164, 613
MunI CAATTG 1 cut(s) 168
MvaI CCWGG 2 cut(s) 164, 613
MwoI GCNNNNNNNGC 5 cut(s) 100, 164, 479, 639, 665
NcoI CCATGG 1 cut(s) 1041
NdeI CATATG 2 cut(s) 79, 1072
NdeII GATC 3 cut(s) 732, 999, 1096
NheI GCTAGC 1 cut(s) 691
NlaIII CATG 6 cut(s) 43, 739, 745, 879, 1013, 1045
NmeAIII GCCGAG 1 cut(s) 79
NsbI TGCGCA 1 cut(s) 460
NsiI ATGCAT 1 cut(s) 670
PaeR7I CTCGAG 2 cut(s) 18, 598
PkrI GCNGC 5 cut(s) 30, 394, 690, 897, 1126
PleI GAGTC 2 cut(s) 581, 590
PpsI GAGTC 2 cut(s) 581, 590
PshBI ATTAAT 1 cut(s) 1167
PsiI TTATAA 1 cut(s) 710
Psp6I CCWGG 2 cut(s) 162, 611
PspGI CCWGG 2 cut(s) 162, 611
PspXI VCTCGAGB 1 cut(s) 598
PstI CTGCAG 1 cut(s) 30
PvuII CAGCTG 2 cut(s) 31, 94
RsaI GTAC 5 cut(s) 194, 237, 316, 546, 623
RsaNI GTAC 5 cut(s) 193, 236, 315, 545, 622
RseI CAYNNNNRTG 2 cut(s) 740, 746
SaqAI TTAA 3 cut(s) 114, 872, 1167
SatI GCNGC 5 cut(s) 29, 393, 689, 896, 1125
Sau3AI GATC 3 cut(s) 732, 999, 1096
ScaI AGTACT 2 cut(s) 194, 316
SchI GAGTC 2 cut(s) 581, 590
ScrFI CCNGG 2 cut(s) 164, 613
SfaNI GCATC 4 cut(s) 45, 368, 470, 662
SfcI CTRYAG 2 cut(s) 26, 318
Sfr274I CTCGAG 2 cut(s) 18, 598
SlaI CTCGAG 2 cut(s) 18, 598
SmiMI CAYNNNNRTG 2 cut(s) 740, 746
SmlI CTYRAG 2 cut(s) 18, 598
SmoI CTYRAG 2 cut(s) 18, 598
SpeI ACTAGT 1 cut(s) 232
Sse9I AATT 4 cut(s) 168, 363, 868, 952
SspMI CTAG 5 cut(s) 233, 569, 692, 798, 992
StyD4I CCNGG 2 cut(s) 162, 611
StyI CCWWGG 1 cut(s) 1041
TaaI ACNGT 5 cut(s) 145, 192, 299, 342, 646
TaqI TCGA 4 cut(s) 19, 134, 599, 1092
TasI AATT 4 cut(s) 168, 363, 868, 952
TatI WGTACW 5 cut(s) 192, 235, 314, 544, 621
Tru1I TTAA 3 cut(s) 114, 872, 1167
Tru9I TTAA 3 cut(s) 114, 872, 1167
TscAI CASTG 1 cut(s) 150
TseI GCWGC 5 cut(s) 28, 392, 688, 895, 1124
TspDTI ATGAA 7 cut(s) 75, 213, 625, 688, 881, 1059, 1149
TspRI CASTG 1 cut(s) 150
VspI ATTAAT 1 cut(s) 1167
XapI RAATTY 2 cut(s) 868, 952
XcmI CCANNNNNNNNNTGG 1 cut(s) 913
XhoI CTCGAG 2 cut(s) 18, 598
XmiI GTMKAC 1 cut(s) 344
XspI CTAG 5 cut(s) 233, 569, 692, 798, 992
ZrmI AGTACT 2 cut(s) 194, 316
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.