AT4G18197

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002718
Physical Location & Seq
Forward (+)
10071481 .. 10073557
2077 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18197.1

Sequence Viewer

Length: 1173 bp
ATGGACAGATCTCAAGAACACTATGCCAATGGTGACCAGAACTTAGAAGCAAACCTTATAGATCATGAGGTGGTAACTGAATCATCATCATCAGCTGTGCCTCAAACCGAGAACTATAAAAGGTGGCTTCGTGTCTCCATATACGTAATCTTTGTCCTCTTTTGCCAGCCACTAGCTACAATTCTGGGTAGATTGTACTATGAAAATGGAGGAAATAGCACATATGTGGTAACACTTCTTCAACTCATTGGCTTCCCTGTACTGGTTCTGTTCCGCTTCTTTTCTCGAATCAGGCAACCCAAATCAACAGATACAAATTTCAGTCAGTCCCCTTCCTTCACCACCCTTGCATCGGTTTACTTGTGCACTGGACTGCTAGTGTCCGCTTATGCTTATTTGTCTGCAGTAGGGTTGCTCTACTTACCAGTCTCTACTTTCTCCCTCATCTTGGCCTCACAGTTGGCCTTCACTGCCTTTTTCTCATATTTCCTTAACTCGCAAAAGTTCACTCCTTTGATAGTCAGTTCTTTGCTTCTCCTCACTGTATCCTCTGCTCTTCTTGTGGTCAACACTGATTCAGAAAACTCAACTAATGTATCTAGAGTACAGTATGTGATCGGGTTCATATGTACCATCGGTGCTTCCGCTGGGATTGGACTGTTACTATCTCTGATACAAATGCTCTTCAGGAAAGTTTTCACGAAGCATACATCCTCAGCAGTCACGGACTTGGCCATTTACCAGTCTCTAGTTGCGAGTTGTGTAGTTCTCATAGGACTTTTTGCAAGTGGAGAGTGGGAAACTTTGCCAAGTGAGATGAGAAACTACAAACTCGGGAAAGTGTCATATGTTTTGACTTTAGCCTCGGCAGCTATTTCCTGGCAAGTCTACACTCTTGGTCTTGTGGGATTGATCTTCGAGTCATCCTCTGTGTTCTCCAATTCCATAACAGCTGTGGGATTGCCTATAGTTCCAGTTGCGGCAGTGATAGTTTTCCATGATAGAATGGACGCATCAAAAATCTTCTCCATTATTTTAGCTATCTGCGGCTTCCTTTCATTCGTCTATCAGCACTACCTCGACGAAAAGAAGTTGAATACTAGCCACACAAGTGCTGTAGGAGATCTTCATCTACCTGTTGAGGAAGGTCACACAAACATACAAAGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

390

Amino Acids

42.8

Weight (kDa)

6.23

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 44 - 364 7.2e-117 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 888
AciI CCGC 5 cut(s) 274, 384, 645, 980, 1047
AcoI YGGCCR 1 cut(s) 732
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 1 cut(s) 670
AfaI GTAC 4 cut(s) 197, 261, 606, 631
AfiI CCNNNNNNNGG 3 cut(s) 262, 352, 448
AgsI TTSAA 2 cut(s) 242, 1096
AjnI CCWGG 1 cut(s) 878
AleI CACNNNNGTG 2 cut(s) 224, 1110
AluBI AGCT 5 cut(s) 95, 176, 872, 953, 1040
AluI AGCT 5 cut(s) 95, 176, 872, 953, 1040
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 3 cut(s) 139, 433, 750
Alw44I GTGCAC 1 cut(s) 364
Ama87I CYCGRG 1 cut(s) 833
AoxI GGCC 3 cut(s) 450, 462, 732
ApaLI GTGCAC 1 cut(s) 364
ApeKI GCWGC 1 cut(s) 869
ApoI RAATTY 1 cut(s) 316
Asp700I GAANNNNTTC 1 cut(s) 695
AsuHPI GGTGA 2 cut(s) 44, 331
AvaI CYCGRG 1 cut(s) 833
BaeGI GKGCMC 1 cut(s) 368
BaeI ACNNNNGTAYC 2 cut(s) 579, 612
BalI TGGCCA 1 cut(s) 734
BarI GAAGNNNNNNTAC 4 cut(s) 222, 254, 1110, 1142
Bbv12I GWGCWC 1 cut(s) 368
BbvCI CCTCAGC 1 cut(s) 715
BbvI GCAGC 1 cut(s) 881
BccI CCATC 1 cut(s) 641
BciT130I CCWGG 1 cut(s) 880
BciVI GTATCC 1 cut(s) 556
BcoDI GTCTC 3 cut(s) 139, 433, 750
BfaI CTAG 5 cut(s) 173, 377, 600, 749, 1101
BfmI CTRYAG 3 cut(s) 402, 966, 1116
BfuI GTATCC 1 cut(s) 556
BglII AGATCT 2 cut(s) 8, 1123
BisI GCNGC 3 cut(s) 870, 981, 1048
BlsI GCNGC 3 cut(s) 871, 982, 1049
Bme1390I CCNGG 1 cut(s) 880
BmeT110I CYCGRG 1 cut(s) 833
BmrFI CCNGG 1 cut(s) 880
BmsI GCATC 2 cut(s) 359, 1022
BplI GAGNNNNNCTC 2 cut(s) 911, 943
Bpu10I CCTNAGC 1 cut(s) 715
BsaAI YACGTR 1 cut(s) 145
BsaBI GATNNNNATC 1 cut(s) 1128
BsaJI CCNNGG 1 cut(s) 864
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 352, 448
Bse1I ACTGG 5 cut(s) 267, 373, 425, 742, 974
Bse8I GATNNNNATC 1 cut(s) 1128
BseBI CCWGG 1 cut(s) 880
BseDI CCNNGG 1 cut(s) 864
BseGI GGATG 2 cut(s) 710, 923
BseJI GATNNNNATC 1 cut(s) 1128
BseLI CCNNNNNNNGG 3 cut(s) 262, 352, 448
BseMII CTCAG 1 cut(s) 729
BseNI ACTGG 5 cut(s) 267, 373, 425, 742, 974
BseRI GAGGAG 1 cut(s) 527
BseSI GKGCMC 1 cut(s) 368
BseXI GCAGC 1 cut(s) 881
BseYI CCCAGC 1 cut(s) 647
BshFI GGCC 3 cut(s) 452, 464, 734
BsiHKAI GWGCWC 1 cut(s) 368
BsiHKCI CYCGRG 1 cut(s) 833
BslFI GGGAC 1 cut(s) 313
BslI CCNNNNNNNGG 3 cut(s) 262, 352, 448
BsmAI GTCTC 3 cut(s) 139, 433, 750
BsmFI GGGAC 1 cut(s) 313
BsnI GGCC 3 cut(s) 452, 464, 734
BsoBI CYCGRG 1 cut(s) 833
Bsp1286I GDGCHC 1 cut(s) 368
Bsp143I GATC 5 cut(s) 8, 61, 615, 912, 1123
BspACI CCGC 5 cut(s) 274, 384, 645, 980, 1047
BspANI GGCC 3 cut(s) 452, 464, 734
BspCNI CTCAG 1 cut(s) 728
BspHI TCATGA 1 cut(s) 64
BspMAI CTGCAG 1 cut(s) 406
BspQI GCTCTTC 2 cut(s) 561, 689
BsrI ACTGG 5 cut(s) 267, 373, 425, 742, 974
BssECI CCNNGG 1 cut(s) 864
BssMI GATC 5 cut(s) 8, 61, 615, 912, 1123
Bst2UI CCWGG 1 cut(s) 880
Bst4CI ACNGT 4 cut(s) 459, 544, 609, 660
Bst6I CTCTTC 2 cut(s) 561, 689
BstBAI YACGTR 1 cut(s) 145
BstC8I GCNNGC 1 cut(s) 167
BstDEI CTNAG 2 cut(s) 43, 715
BstEII GGTNACC 1 cut(s) 32
BstF5I GGATG 2 cut(s) 710, 923
BstKTI GATC 5 cut(s) 11, 64, 618, 915, 1126
BstMAI GTCTC 3 cut(s) 139, 433, 750
BstMBI GATC 5 cut(s) 8, 61, 615, 912, 1123
BstMWI GCNNNNNNNGC 2 cut(s) 470, 869
BstNI CCWGG 1 cut(s) 880
BstPI GGTNACC 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 878
BstSFI CTRYAG 3 cut(s) 402, 966, 1116
BstSLI GKGCMC 1 cut(s) 368
BstSNI TACGTA 1 cut(s) 145
BstV1I GCAGC 1 cut(s) 881
BstX2I RGATCY 2 cut(s) 8, 1123
BstYI RGATCY 2 cut(s) 8, 1123
BsuI GTATCC 1 cut(s) 556
BsuRI GGCC 3 cut(s) 452, 464, 734
BtsCI GGATG 2 cut(s) 710, 923
BtsI GCAGTG 2 cut(s) 468, 990
BtsIMutI CAGTG 5 cut(s) 366, 468, 540, 570, 990
Cac8I GCNNGC 1 cut(s) 167
CciI TCATGA 1 cut(s) 64
CseI GACGC 1 cut(s) 1019
Csp6I GTAC 4 cut(s) 196, 260, 605, 630
CviAII CATG 2 cut(s) 65, 998
CviQI GTAC 4 cut(s) 196, 260, 605, 630
DdeI CTNAG 2 cut(s) 43, 715
DpnI GATC 5 cut(s) 10, 63, 617, 914, 1125
DpnII GATC 5 cut(s) 8, 61, 615, 912, 1123
EaeI YGGCCR 1 cut(s) 732
Eam1104I CTCTTC 2 cut(s) 561, 689
EarI CTCTTC 2 cut(s) 561, 689
Eco105I TACGTA 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 670
Eco88I CYCGRG 1 cut(s) 833
Eco91I GGTNACC 1 cut(s) 32
EcoO65I GGTNACC 1 cut(s) 32
EcoRII CCWGG 1 cut(s) 878
FaeI CATG 2 cut(s) 68, 1001
FalI AAGNNNNNCTT 2 cut(s) 39, 71
FaqI GGGAC 1 cut(s) 313
FatI CATG 2 cut(s) 64, 997
FauNDI CATATG 3 cut(s) 223, 626, 847
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 3 cut(s) 870, 981, 1048
FokI GGATG 2 cut(s) 697, 910
Fsp4HI GCNGC 3 cut(s) 870, 981, 1048
FspBI CTAG 5 cut(s) 173, 377, 600, 749, 1101
GluI GCNGC 3 cut(s) 870, 981, 1048
GsaI CCCAGC 1 cut(s) 651
HaeIII GGCC 3 cut(s) 452, 464, 734
HgaI GACGC 1 cut(s) 1019
Hin1II CATG 2 cut(s) 68, 1001
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HinfI GANTC 4 cut(s) 80, 288, 575, 920
HphI GGTGA 2 cut(s) 44, 331
Hpy166II GTNNAC 5 cut(s) 358, 366, 507, 568, 889
Hpy188I TCNGA 2 cut(s) 580, 672
Hpy188III TCNNGA 7 cut(s) 14, 65, 285, 600, 688, 700, 835
Hpy8I GTNNAC 5 cut(s) 358, 366, 507, 568, 889
Hpy99I CGWCG 1 cut(s) 1085
HpyAV CCTTC 4 cut(s) 342, 346, 475, 1139
HpyCH4III ACNGT 4 cut(s) 459, 544, 609, 660
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 4 cut(s) 350, 366, 404, 785
HpyF10VI GCNNNNNNNGC 2 cut(s) 470, 869
HpyF3I CTNAG 2 cut(s) 43, 715
HpySE526I ACGT 1 cut(s) 144
Hsp92II CATG 2 cut(s) 68, 1001
Kzo9I GATC 5 cut(s) 8, 61, 615, 912, 1123
LguI GCTCTTC 2 cut(s) 561, 689
Lsp1109I GCAGC 1 cut(s) 881
LweI GCATC 2 cut(s) 359, 1022
MaeI CTAG 5 cut(s) 173, 377, 600, 749, 1101
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 6 cut(s) 32, 73, 229, 660, 721, 1148
MalI GATC 5 cut(s) 10, 63, 617, 914, 1125
MboI GATC 5 cut(s) 8, 61, 615, 912, 1123
MboII GAAGA 6 cut(s) 230, 548, 676, 907, 1015, 1118
MflI RGATCY 2 cut(s) 8, 1123
MhlI GDGCHC 1 cut(s) 368
MlsI TGGCCA 1 cut(s) 734
MluCI AATT 3 cut(s) 180, 316, 940
MluNI TGGCCA 1 cut(s) 734
MlyI GAGTC 1 cut(s) 929
Mox20I TGGCCA 1 cut(s) 734
MroXI GAANNNNTTC 1 cut(s) 695
MscI TGGCCA 1 cut(s) 734
MseI TTAA 1 cut(s) 492
MslI CAYNNNNRTG 2 cut(s) 224, 1110
Msp20I TGGCCA 1 cut(s) 734
MspA1I CMGCKG 3 cut(s) 95, 647, 953
MspR9I CCNGG 1 cut(s) 880
MvaI CCWGG 1 cut(s) 880
MwoI GCNNNNNNNGC 2 cut(s) 470, 869
NdeI CATATG 3 cut(s) 223, 626, 847
NdeII GATC 5 cut(s) 8, 61, 615, 912, 1123
NlaIII CATG 2 cut(s) 68, 1001
NmeAIII GCCGAG 1 cut(s) 845
NmuCI GTSAC 3 cut(s) 32, 721, 1148
OliI CACNNNNGTG 2 cut(s) 224, 1110
PagI TCATGA 1 cut(s) 64
PciSI GCTCTTC 2 cut(s) 561, 689
PdmI GAANNNNTTC 1 cut(s) 695
PfeI GAWTC 3 cut(s) 80, 288, 575
PkrI GCNGC 3 cut(s) 871, 982, 1049
PleI GAGTC 1 cut(s) 928
PpsI GAGTC 1 cut(s) 928
Ppu21I YACGTR 1 cut(s) 145
Psp6I CCWGG 1 cut(s) 878
PspEI GGTNACC 1 cut(s) 32
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 1 cut(s) 878
PstI CTGCAG 1 cut(s) 406
PsuI RGATCY 2 cut(s) 8, 1123
PvuII CAGCTG 2 cut(s) 95, 953
RsaI GTAC 4 cut(s) 197, 261, 606, 631
RsaNI GTAC 4 cut(s) 196, 260, 605, 630
RseI CAYNNNNRTG 2 cut(s) 224, 1110
SapI GCTCTTC 2 cut(s) 561, 689
SaqAI TTAA 1 cut(s) 492
SatI GCNGC 3 cut(s) 870, 981, 1048
Sau3AI GATC 5 cut(s) 8, 61, 615, 912, 1123
SchI GAGTC 1 cut(s) 929
ScrFI CCNGG 1 cut(s) 880
SduI GDGCHC 1 cut(s) 368
SfaNI GCATC 2 cut(s) 359, 1022
SfcI CTRYAG 3 cut(s) 402, 966, 1116
SmiMI CAYNNNNRTG 2 cut(s) 224, 1110
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
SnaBI TACGTA 1 cut(s) 145
Sse9I AATT 3 cut(s) 180, 316, 940
SsiI CCGC 5 cut(s) 274, 384, 645, 980, 1047
SspMI CTAG 5 cut(s) 173, 377, 600, 749, 1101
StyD4I CCNGG 1 cut(s) 878
TaaI ACNGT 4 cut(s) 459, 544, 609, 660
TaiI ACGT 1 cut(s) 147
TaqI TCGA 3 cut(s) 286, 918, 1080
TasI AATT 3 cut(s) 180, 316, 940
TatI WGTACW 3 cut(s) 195, 259, 604
TauI GCSGC 2 cut(s) 983, 1050
TfiI GAWTC 3 cut(s) 80, 288, 575
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 5 cut(s) 373, 475, 547, 577, 990
TseFI GTSAC 3 cut(s) 32, 721, 1148
TseI GCWGC 1 cut(s) 869
Tsp45I GTSAC 3 cut(s) 32, 721, 1148
TspDTI ATGAA 4 cut(s) 216, 613, 1047, 1118
TspGWI ACGGA 1 cut(s) 740
TspRI CASTG 5 cut(s) 373, 475, 547, 577, 990
VneI GTGCAC 1 cut(s) 364
XapI RAATTY 1 cut(s) 316
XbaI TCTAGA 1 cut(s) 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 952
XmiI GTMKAC 1 cut(s) 888
XmnI GAANNNNTTC 1 cut(s) 695
XspI CTAG 5 cut(s) 173, 377, 600, 749, 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.