pycom05g26700

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002718
Physical Location & Seq
Reverse (-)
27857689 .. 27858740
1052 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g26700.2

Sequence Viewer

Length: 744 bp
ATGGGAGAAGCTCAAGAACTTCAGCTCAGCATCGGGGTTAAGGAAGCAAATGGAGAAGATGAAACAAATTTATCAGGGCCTCCAGATGCCTCCGACCGATCACTGCTACCGCGGCGCAGAGGCGTCAACTGGTGGATTAGAATCGCCATTTACTCGTTCCTCGTCATTGCCGGCCAGTCAGTTGCAACGTTCTTGGGGAAACAGTACTACGACAAAGGCGCCAAAAGCAATTGGCTGGCGACAGTAGTACAACTTTGTGGCTTCCCTATCATGCTTCCCTACTATTTTATCCCAGCAAGCAGAAATAATCCAACCCCAAAAGGTAGTCCTATCCCATCAAAACCACCGTCTACCAAAGTCCTCGCATCAGTATATGTCTCTCTTGGCTTACTGATAGCCCTAGACTGCTACCTGTATTCCGTAGGACTATCTTACCTTCCTGTGTCTACTTATTCCCTCATTTGTGCATCTCAGTTGGCCTTCAACGCGTTGTTCTCATTCTTCCTCAACGCGCAAAAGTTCACTGCTTACATTGTGAATTCTCTAGTTCTCCTCACCATCTCCTCCACCCTCCTCGCGTTCCAAGGTGAAGACGACTCTGGAGGCGATCGATCCAAGTGGAGGGTCGAAGGTCAAGTATGCAATTGGGTTCCTATGCACTTTAGGGGCATCTGCCGGATACGGATTAACGCTCTCCCTAACTCAGCTTGCCTTCAAAAGGGTTATAAAAAGGGAGACGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

248

Amino Acids

27.16

Weight (kDa)

9.1

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
AccB1I GGYRCC 1 cut(s) 218
AccI GTMKAC 2 cut(s) 350, 446
AccII CGCG 4 cut(s) 112, 488, 512, 578
AciI CCGC 2 cut(s) 110, 112
AclI AACGTT 1 cut(s) 188
AclWI GGATC 1 cut(s) 606
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 2 cut(s) 67, 538
AcuI CTGAAG 1 cut(s) 5
AcyI GRCGYC 2 cut(s) 123, 219
AfaI GTAC 2 cut(s) 206, 249
AfiI CCNNNNNNNGG 2 cut(s) 621, 718
AflIII ACRYGT 1 cut(s) 486
AgsI TTSAA 2 cut(s) 484, 716
AluBI AGCT 3 cut(s) 11, 25, 707
AluI AGCT 3 cut(s) 11, 25, 707
Alw26I GTCTC 2 cut(s) 382, 729
AlwI GGATC 1 cut(s) 606
AoxI GGCC 3 cut(s) 77, 172, 477
ApoI RAATTY 2 cut(s) 67, 538
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AspLEI GCGC 3 cut(s) 117, 221, 514
AspS9I GGNCC 1 cut(s) 77
AsuHPI GGTGA 2 cut(s) 547, 599
BanI GGYRCC 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 597
BccI CCATC 2 cut(s) 343, 566
BciVI GTATCC 1 cut(s) 672
BcoDI GTCTC 2 cut(s) 382, 729
BfaI CTAG 2 cut(s) 401, 545
BfoI RGCGCY 1 cut(s) 222
BfuI GTATCC 1 cut(s) 672
BisI GCNGC 1 cut(s) 113
BlpI GCTNAGC 1 cut(s) 26
BlsI GCNGC 1 cut(s) 114
BmcAI AGTACT 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 2 cut(s) 220, 651
BmsI GCATC 5 cut(s) 39, 76, 374, 476, 678
BpiI GAAGAC 1 cut(s) 597
BpmI CTGGAG 2 cut(s) 66, 621
Bpu1102I GCTNAGC 1 cut(s) 26
Bsa29I ATCGAT 1 cut(s) 610
BsaBI GATNNNNATC 1 cut(s) 140
BsaHI GRCGYC 2 cut(s) 123, 219
BsaJI CCNNGG 2 cut(s) 110, 583
Bsc4I CCNNNNNNNGG 2 cut(s) 621, 718
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 2 cut(s) 134, 175
Bse3DI GCAATG 1 cut(s) 165
Bse8I GATNNNNATC 1 cut(s) 140
BseCI ATCGAT 1 cut(s) 610
BseDI CCNNGG 2 cut(s) 110, 583
BseJI GATNNNNATC 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 621, 718
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 3 cut(s) 40, 485, 717
BseNI ACTGG 2 cut(s) 134, 175
BseRI GAGGAG 3 cut(s) 542, 553, 563
BseYI CCCAGC 1 cut(s) 292
Bsh1236I CGCG 4 cut(s) 112, 488, 512, 578
Bsh1285I CGRYCG 2 cut(s) 97, 610
BshFI GGCC 3 cut(s) 79, 174, 479
BshNI GGYRCC 1 cut(s) 218
BshVI ATCGAT 1 cut(s) 610
BsiEI CGRYCG 2 cut(s) 97, 610
BsiSI CCGG 2 cut(s) 171, 676
BslI CCNNNNNNNGG 2 cut(s) 621, 718
BsmAI GTCTC 2 cut(s) 382, 729
BsmBI CGTCTC 1 cut(s) 729
BsnI GGCC 3 cut(s) 79, 174, 479
Bsp143I GATC 3 cut(s) 98, 607, 611
Bsp1720I GCTNAGC 1 cut(s) 26
BspACI CCGC 2 cut(s) 110, 112
BspANI GGCC 3 cut(s) 79, 174, 479
BspCNI CTCAG 3 cut(s) 39, 484, 716
BspDI ATCGAT 1 cut(s) 610
BspFNI CGCG 4 cut(s) 112, 488, 512, 578
BspLI GGNNCC 2 cut(s) 220, 651
BspPI GGATC 1 cut(s) 606
BspT107I GGYRCC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 170
BsrI ACTGG 2 cut(s) 134, 175
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 2 cut(s) 110, 583
BssMI GATC 3 cut(s) 98, 607, 611
BssNI GRCGYC 2 cut(s) 123, 219
BssT1I CCWWGG 1 cut(s) 583
Bst4CI ACNGT 3 cut(s) 204, 244, 348
BstACI GRCGYC 2 cut(s) 123, 219
BstC8I GCNNGC 4 cut(s) 172, 237, 298, 709
BstDEI CTNAG 3 cut(s) 26, 471, 703
BstDSI CCRYGG 1 cut(s) 110
BstENI CCTNNNNNAGG 1 cut(s) 716
BstFNI CGCG 4 cut(s) 112, 488, 512, 578
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 3 cut(s) 117, 221, 514
BstKTI GATC 3 cut(s) 101, 610, 614
BstMAI GTCTC 2 cut(s) 382, 729
BstMBI GATC 3 cut(s) 98, 607, 611
BstMCI CGRYCG 2 cut(s) 97, 610
BstMWI GCNNNNNNNGC 3 cut(s) 112, 225, 485
BstUI CGCG 4 cut(s) 112, 488, 512, 578
BstV2I GAAGAC 1 cut(s) 597
Bsu15I ATCGAT 1 cut(s) 610
BsuI GTATCC 1 cut(s) 672
BsuRI GGCC 3 cut(s) 79, 174, 479
BsuTUI ATCGAT 1 cut(s) 610
BtgI CCRYGG 1 cut(s) 110
BtsI GCAGTG 2 cut(s) 101, 522
BtsIMutI CAGTG 2 cut(s) 101, 522
Cac8I GCNNGC 4 cut(s) 172, 237, 298, 709
CfoI GCGC 3 cut(s) 117, 221, 514
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 1 cut(s) 77
Cfr42I CCGCGG 1 cut(s) 113
ClaI ATCGAT 1 cut(s) 610
CseI GACGC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 205, 248
CviAII CATG 1 cut(s) 271
CviQI GTAC 2 cut(s) 205, 248
DdeI CTNAG 3 cut(s) 26, 471, 703
DinI GGCGCC 1 cut(s) 220
DpnI GATC 3 cut(s) 100, 609, 613
DpnII GATC 3 cut(s) 98, 607, 611
EaeI YGGCCR 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 583
Eco57I CTGAAG 1 cut(s) 5
EcoNI CCTNNNNNAGG 1 cut(s) 716
EcoO109I RGGNCCY 1 cut(s) 77
EcoRI GAATTC 1 cut(s) 538
EcoT14I CCWWGG 1 cut(s) 583
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 583
Esp3I CGTCTC 1 cut(s) 729
FaeI CATG 1 cut(s) 274
FaiI YATR 6 cut(s) 272, 373, 375, 640, 656, 726
FatI CATG 1 cut(s) 270
FblI GTMKAC 2 cut(s) 350, 446
Fnu4HI GCNGC 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 113
FspBI CTAG 2 cut(s) 401, 545
GlaI GCGC 3 cut(s) 116, 220, 513
GluI GCNGC 1 cut(s) 113
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 2 cut(s) 66, 621
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 3 cut(s) 79, 174, 479
HapII CCGG 2 cut(s) 171, 676
HgaI GACGC 1 cut(s) 112
HhaI GCGC 3 cut(s) 117, 221, 514
Hin1I GRCGYC 2 cut(s) 123, 219
Hin1II CATG 1 cut(s) 274
Hin6I GCGC 3 cut(s) 115, 219, 512
HinP1I GCGC 3 cut(s) 115, 219, 512
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 2 cut(s) 141, 596
HpaII CCGG 2 cut(s) 171, 676
HphI GGTGA 2 cut(s) 547, 599
Hpy166II GTNNAC 4 cut(s) 127, 351, 447, 522
Hpy188I TCNGA 1 cut(s) 94
Hpy188III TCNNGA 3 cut(s) 14, 83, 600
Hpy8I GTNNAC 4 cut(s) 127, 351, 447, 522
HpyAV CCTTC 4 cut(s) 446, 490, 623, 722
HpyCH4III ACNGT 3 cut(s) 204, 244, 348
HpyCH4IV ACGT 2 cut(s) 188, 738
HpyCH4V TGCA 4 cut(s) 185, 467, 642, 658
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 225, 485
HpyF3I CTNAG 3 cut(s) 26, 471, 703
HpySE526I ACGT 2 cut(s) 188, 738
Hsp92I GRCGYC 2 cut(s) 123, 219
Hsp92II CATG 1 cut(s) 274
HspAI GCGC 3 cut(s) 115, 219, 512
KasI GGCGCC 1 cut(s) 218
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
KspI CCGCGG 1 cut(s) 113
Kzo9I GATC 3 cut(s) 98, 607, 611
LweI GCATC 5 cut(s) 39, 76, 374, 476, 678
MaeI CTAG 2 cut(s) 401, 545
MaeII ACGT 2 cut(s) 188, 738
MalI GATC 3 cut(s) 100, 609, 613
MboI GATC 3 cut(s) 98, 607, 611
MboII GAAGA 3 cut(s) 68, 493, 602
MfeI CAATTG 2 cut(s) 229, 643
MluCI AATT 4 cut(s) 67, 229, 538, 643
MluI ACGCGT 1 cut(s) 486
Mly113I GGCGCC 1 cut(s) 219
MlyI GAGTC 1 cut(s) 590
MmeI TCCRAC 2 cut(s) 117, 335
MroNI GCCGGC 1 cut(s) 170
MseI TTAA 2 cut(s) 39, 687
MspA1I CMGCKG 1 cut(s) 112
MspI CCGG 2 cut(s) 171, 676
MunI CAATTG 2 cut(s) 229, 643
MvnI CGCG 4 cut(s) 112, 488, 512, 578
MwoI GCNNNNNNNGC 3 cut(s) 112, 225, 485
NaeI GCCGGC 1 cut(s) 172
NarI GGCGCC 1 cut(s) 219
NdeII GATC 3 cut(s) 98, 607, 611
NgoMIV GCCGGC 1 cut(s) 170
NlaIII CATG 1 cut(s) 274
NlaIV GGNNCC 2 cut(s) 220, 651
PdiI GCCGGC 1 cut(s) 172
PfeI GAWTC 1 cut(s) 141
PkrI GCNGC 1 cut(s) 114
Ple19I CGATCG 1 cut(s) 610
PleI GAGTC 1 cut(s) 590
PluTI GGCGCC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 590
PsiI TTATAA 1 cut(s) 726
Psp1406I AACGTT 1 cut(s) 188
PspFI CCCAGC 1 cut(s) 292
PspN4I GGNNCC 2 cut(s) 220, 651
PspPI GGNCC 1 cut(s) 77
PvuI CGATCG 1 cut(s) 610
RsaI GTAC 2 cut(s) 206, 249
RsaNI GTAC 2 cut(s) 205, 248
SacII CCGCGG 1 cut(s) 113
SaqAI TTAA 2 cut(s) 39, 687
SatI GCNGC 1 cut(s) 113
Sau3AI GATC 3 cut(s) 98, 607, 611
Sau96I GGNCC 1 cut(s) 77
ScaI AGTACT 1 cut(s) 206
SchI GAGTC 1 cut(s) 590
SfaNI GCATC 5 cut(s) 39, 76, 374, 476, 678
SfoI GGCGCC 1 cut(s) 220
Sfr303I CCGCGG 1 cut(s) 113
SgrBI CCGCGG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
Sse9I AATT 4 cut(s) 67, 229, 538, 643
SsiI CCGC 2 cut(s) 110, 112
SspDI GGCGCC 1 cut(s) 218
SspMI CTAG 2 cut(s) 401, 545
StyI CCWWGG 1 cut(s) 583
TaaI ACNGT 3 cut(s) 204, 244, 348
TaiI ACGT 2 cut(s) 191, 741
TaqI TCGA 2 cut(s) 610, 627
TaqII GACCGA 1 cut(s) 111
TasI AATT 4 cut(s) 67, 229, 538, 643
TatI WGTACW 2 cut(s) 204, 247
TauI GCSGC 1 cut(s) 115
TfiI GAWTC 1 cut(s) 141
Tru1I TTAA 2 cut(s) 39, 687
Tru9I TTAA 2 cut(s) 39, 687
TscAI CASTG 2 cut(s) 108, 529
TspDTI ATGAA 1 cut(s) 75
TspGWI ACGGA 2 cut(s) 409, 697
TspRI CASTG 2 cut(s) 108, 529
XagI CCTNNNNNAGG 1 cut(s) 716
XapI RAATTY 2 cut(s) 67, 538
XmiI GTMKAC 2 cut(s) 350, 446
XspI CTAG 2 cut(s) 401, 545
ZrmI AGTACT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.