Rh5AG102000

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002718
Physical Location & Seq
Forward (+)
9441961 .. 9442677
717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG102000.1

Sequence Viewer

Length: 717 bp
ATGTCGATTTATACAGTCTTTGTCCTCGCTGGCCAGGCAGTTGCAACACTATTGGGAAGACAGTACTATGACAAAGGTGGTAAAAGCAACTGGCTTGCTACACTAGTACAACTTTGTGGCTTTCCTATCTTCCTTCCGTATTATTGCATTCCAGCATTGAACAAAAACTGTCCAACCACAAACAGTACTATAGCAATCGAGTCAAAGCCACCGTCTATATTAGTCCTTGCATCAATTTATTCCTCTCTTGGCTCCCTTATAGCAGCAGACTACTACTTGTATTCAGTAGGACTATCTTACCTTCCTGTATCTACTTTCTCCCTCATTTCTGCATCCCAACTCACCTTCAATGCACTTTTCTCCTTCTTCCTCAACTCGCAGAAGTTAACTTTTTTAATTGTCAACTCTCTTTTTATACTGACCATGTCATCCAGCCTCCTAGTGCTTCAAGCAGACCACTCAGCAGACTCGAGTAAAGTTTCCAGGTCAAAGTACATAATAGGCTTTGTCTGCACTGTTGGGGCATCGGCTGGATATGCATTGACACTTTCTCTAACGCAGCTAGTCTTCATAAAGGTTATAAGAAAAGAAACATTTAGAGTGATCATGGATATGACAGTGTATACATCAGTCATTGCAACCGCTATTATCTTGGTGGGACTTTTTGCTAGTGGAGACCAGAAGGGTCTAAAGCATGAGATGGAGGATTTCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

238

Amino Acids

25.92

Weight (kDa)

8.81

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 2 - 237 3.6e-76 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 581
AccI GTMKAC 1 cut(s) 623
AciI CCGC 1 cut(s) 642
AcoI YGGCCR 1 cut(s) 31
AfaI GTAC 4 cut(s) 65, 108, 187, 494
AgsI TTSAA 3 cut(s) 160, 349, 449
AhlI ACTAGT 1 cut(s) 103
AjnI CCWGG 2 cut(s) 33, 482
AluBI AGCT 1 cut(s) 562
AluI AGCT 1 cut(s) 562
Alw26I GTCTC 1 cut(s) 669
Ama87I CYCGRG 1 cut(s) 469
AoxI GGCC 1 cut(s) 31
ApeKI GCWGC 2 cut(s) 263, 559
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AsuHPI GGTGA 1 cut(s) 334
AvaI CYCGRG 1 cut(s) 469
BalI TGGCCA 1 cut(s) 33
BbsI GAAGAC 2 cut(s) 64, 559
BbvI GCAGC 2 cut(s) 275, 571
BccI CCATC 1 cut(s) 694
BciT130I CCWGG 2 cut(s) 35, 484
BclI TGATCA 1 cut(s) 603
BcoDI GTCTC 1 cut(s) 669
BcuI ACTAGT 1 cut(s) 103
BfaI CTAG 4 cut(s) 104, 440, 563, 669
BfmI CTRYAG 1 cut(s) 189
BisI GCNGC 2 cut(s) 264, 560
BlsI GCNGC 2 cut(s) 265, 561
BmcAI AGTACT 2 cut(s) 65, 187
Bme1390I CCNGG 2 cut(s) 35, 484
BmeT110I CYCGRG 1 cut(s) 469
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 2 cut(s) 35, 484
BmsI GCATC 3 cut(s) 239, 341, 533
BpiI GAAGAC 2 cut(s) 64, 559
BsaI GGTCTC 1 cut(s) 669
Bse1I ACTGG 1 cut(s) 95
Bse3DI GCAATG 1 cut(s) 633
BseBI CCWGG 2 cut(s) 35, 484
BseGI GGATG 2 cut(s) 332, 428
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 1 cut(s) 95
BseXI GCAGC 2 cut(s) 275, 571
BsgI GTGCAG 1 cut(s) 496
BshFI GGCC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 469
BslFI GGGAC 1 cut(s) 672
BsmAI GTCTC 1 cut(s) 669
BsmFI GGGAC 1 cut(s) 672
BsmI GAATGC 1 cut(s) 147
BsnI GGCC 1 cut(s) 33
Bso31I GGTCTC 1 cut(s) 669
BsoBI CYCGRG 1 cut(s) 469
Bsp143I GATC 1 cut(s) 603
BspACI CCGC 1 cut(s) 642
BspANI GGCC 1 cut(s) 33
BspCNI CTCAG 1 cut(s) 473
BspLI GGNNCC 1 cut(s) 253
BspTNI GGTCTC 1 cut(s) 669
BsrDI GCAATG 1 cut(s) 633
BsrI ACTGG 1 cut(s) 95
BssMI GATC 1 cut(s) 603
BssNAI GTATAC 1 cut(s) 624
Bst1107I GTATAC 1 cut(s) 624
Bst2UI CCWGG 2 cut(s) 35, 484
Bst4CI ACNGT 7 cut(s) 16, 63, 170, 185, 213, 517, 619
BstC8I GCNNGC 2 cut(s) 31, 96
BstDEI CTNAG 1 cut(s) 460
BstF5I GGATG 2 cut(s) 332, 428
BstKTI GATC 1 cut(s) 606
BstMAI GTCTC 1 cut(s) 669
BstMBI GATC 1 cut(s) 603
BstMWI GCNNNNNNNGC 3 cut(s) 35, 510, 536
BstNI CCWGG 2 cut(s) 35, 484
BstSCI CCNGG 2 cut(s) 33, 482
BstSFI CTRYAG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 275, 571
BstV2I GAAGAC 2 cut(s) 64, 559
BstZ17I GTATAC 1 cut(s) 624
BsuRI GGCC 1 cut(s) 33
BtsCI GGATG 2 cut(s) 332, 428
BtsIMutI CAGTG 2 cut(s) 513, 624
Cac8I GCNNGC 2 cut(s) 31, 96
Csp6I GTAC 4 cut(s) 64, 107, 186, 493
CviAII CATG 3 cut(s) 424, 607, 695
CviJI RGCY 9 cut(s) 33, 94, 120, 208, 252, 435, 504, 530, 562
CviKI_1 RGCY 9 cut(s) 33, 94, 120, 208, 252, 435, 504, 530, 562
CviQI GTAC 4 cut(s) 64, 107, 186, 493
DdeI CTNAG 1 cut(s) 460
DpnI GATC 1 cut(s) 605
DpnII GATC 1 cut(s) 603
EaeI YGGCCR 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 669
Eco88I CYCGRG 1 cut(s) 469
EcoRII CCWGG 2 cut(s) 33, 482
EcoT22I ATGCAT 1 cut(s) 541
FaeI CATG 3 cut(s) 427, 610, 698
FaqI GGGAC 1 cut(s) 672
FatI CATG 3 cut(s) 423, 606, 694
FbaI TGATCA 1 cut(s) 603
FblI GTMKAC 1 cut(s) 623
Fnu4HI GCNGC 2 cut(s) 264, 560
FokI GGATG 2 cut(s) 319, 415
Fsp4HI GCNGC 2 cut(s) 264, 560
FspBI CTAG 4 cut(s) 104, 440, 563, 669
GluI GCNGC 2 cut(s) 264, 560
HaeIII GGCC 1 cut(s) 33
Hin1II CATG 3 cut(s) 427, 610, 698
HincII GTYRAC 2 cut(s) 387, 403
HindII GTYRAC 2 cut(s) 387, 403
HinfI GANTC 2 cut(s) 200, 467
HpaI GTTAAC 1 cut(s) 387
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 3 cut(s) 387, 403, 624
Hpy8I GTNNAC 3 cut(s) 387, 403, 624
HpyAV CCTTC 5 cut(s) 143, 311, 355, 373, 676
HpyCH4III ACNGT 7 cut(s) 16, 63, 170, 185, 213, 517, 619
HpyCH4V TGCA 8 cut(s) 44, 147, 230, 332, 353, 513, 539, 638
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 510, 536
HpyF3I CTNAG 1 cut(s) 460
Hsp92II CATG 3 cut(s) 427, 610, 698
Ksp22I TGATCA 1 cut(s) 603
KspAI GTTAAC 1 cut(s) 387
Kzo9I GATC 1 cut(s) 603
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 2 cut(s) 275, 571
LweI GCATC 3 cut(s) 239, 341, 533
MaeI CTAG 4 cut(s) 104, 440, 563, 669
MalI GATC 1 cut(s) 605
MboI GATC 1 cut(s) 603
MboII GAAGA 4 cut(s) 69, 121, 358, 559
MlsI TGGCCA 1 cut(s) 33
MluCI AATT 2 cut(s) 234, 396
MluNI TGGCCA 1 cut(s) 33
MlyI GAGTC 2 cut(s) 209, 461
MmeI TCCRAC 1 cut(s) 197
MnlI CCTC 6 cut(s) 35, 253, 332, 380, 446, 697
Mox20I TGGCCA 1 cut(s) 33
Mph1103I ATGCAT 1 cut(s) 541
MscI TGGCCA 1 cut(s) 33
MseI TTAA 2 cut(s) 386, 395
MslI CAYNNNNRTG 1 cut(s) 611
Msp20I TGGCCA 1 cut(s) 33
MspR9I CCNGG 2 cut(s) 35, 484
Mva1269I GAATGC 1 cut(s) 147
MvaI CCWGG 2 cut(s) 35, 484
MwoI GCNNNNNNNGC 3 cut(s) 35, 510, 536
NdeII GATC 1 cut(s) 603
NlaIII CATG 3 cut(s) 427, 610, 698
NlaIV GGNNCC 1 cut(s) 253
NsiI ATGCAT 1 cut(s) 541
PaeR7I CTCGAG 1 cut(s) 469
PctI GAATGC 1 cut(s) 147
PflFI GACNNNGTC 1 cut(s) 424
PkrI GCNGC 2 cut(s) 265, 561
PleI GAGTC 2 cut(s) 208, 461
PpsI GAGTC 2 cut(s) 208, 461
PsiI TTATAA 1 cut(s) 581
Psp6I CCWGG 2 cut(s) 33, 482
PspGI CCWGG 2 cut(s) 33, 482
PspN4I GGNNCC 1 cut(s) 253
PspXI VCTCGAGB 1 cut(s) 469
PsyI GACNNNGTC 1 cut(s) 424
RsaI GTAC 4 cut(s) 65, 108, 187, 494
RsaNI GTAC 4 cut(s) 64, 107, 186, 493
RseI CAYNNNNRTG 1 cut(s) 611
SaqAI TTAA 2 cut(s) 386, 395
SatI GCNGC 2 cut(s) 264, 560
Sau3AI GATC 1 cut(s) 603
ScaI AGTACT 2 cut(s) 65, 187
SchI GAGTC 2 cut(s) 209, 461
ScrFI CCNGG 2 cut(s) 35, 484
SetI ASST 6 cut(s) 79, 303, 347, 488, 564, 579
SfaNI GCATC 3 cut(s) 239, 341, 533
SfcI CTRYAG 1 cut(s) 189
Sfr274I CTCGAG 1 cut(s) 469
SlaI CTCGAG 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 611
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
SpeI ACTAGT 1 cut(s) 103
Sse9I AATT 2 cut(s) 234, 396
SsiI CCGC 1 cut(s) 642
SspMI CTAG 4 cut(s) 104, 440, 563, 669
StyD4I CCNGG 2 cut(s) 33, 482
TaaI ACNGT 7 cut(s) 16, 63, 170, 185, 213, 517, 619
TaqI TCGA 3 cut(s) 5, 198, 470
TasI AATT 2 cut(s) 234, 396
TatI WGTACW 4 cut(s) 63, 106, 185, 492
Tru1I TTAA 2 cut(s) 386, 395
Tru9I TTAA 2 cut(s) 386, 395
TscAI CASTG 2 cut(s) 520, 624
TseI GCWGC 2 cut(s) 263, 559
TspDTI ATGAA 1 cut(s) 559
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 2 cut(s) 520, 624
Tth111I GACNNNGTC 1 cut(s) 424
XhoI CTCGAG 1 cut(s) 469
XmiI GTMKAC 1 cut(s) 623
XspI CTAG 4 cut(s) 104, 440, 563, 669
ZrmI AGTACT 2 cut(s) 65, 187
Zsp2I ATGCAT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.