MD05G1290000.v1.1

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002718
Physical Location & Seq
Reverse (-)
42190034 .. 42191868
1835 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1290000.v1.1.491

Sequence Viewer

Length: 1182 bp
ATGGGAACAGCTCAAGAACTTCAGCTCAGCATTGGAGTTAAGGAAGCAAATGGAGAAGATGAAACAAATTCATCAGGGCCTTCAGATGCCTCCGACCGATCACTGCTGCCGCGGCGCAGAGGCGTCAACTGGTGGATTAGAATCGCCATTTACTCGTTCCTCGTCATTGCCGGCCAGTCAGTTGCAACGTTCTTGGGAAAACAGTACTACGACAAAGGCGCCAAAAGCAATTGGCTGGCGACAGTAGTACAACTTTGTGGCTTTCCTATCATGCTTCCTTACTATTTCATCCCAGCAAGCAGAAATAATCCAACCCCAAAAGGTAGTCCTATCCCATCAAAACCACCGTCTACCAAAGTCGTCGCATCAGTATATGTCTCTCTTGGCTTCCTGATAGCCCTAGACTGCTACTTGTATTCCGTAGGACTATCTTACCTTCCTGTATCTACTTATTCCCTCATTTGTGCATCTCAGTTGGCCTTCAACGCGTTGTTCTCATTCTTCCTCAACGCGCAAAAGTTCACTGCTTACATTGTGAATTCTCTGGTTCTCCTCACCATCTCCTCCACCCTCCTCGCGTTCCAAGGTGAAGACGAATCTGGAGGCGATGATCCAAGTGGAGGGTCGAAGGTAAAGTATGCAATCGGGTTCCTATGCACTGTAGGGGCATCCGCCGGATACGGATTAACGCTCTCCCTAACTCAGCTTGCCTTCAAAAGGGCTATAAAAAGGGAGACGTTTAGAGCGCTCATGGACATGATAGTGTATCAAAATATGGTTGCAACCTGTTTTACCGTGGTCGGACTTTTCGCTAGCGGAGAGTGGAAGCATTTGAAGGGTGAGATGGAGGGGTATAAGCTAGGGAAGGTATCCTATGTGATGAATTTGACATGGACAGCAATAACATGGCAGGTCTTTTCTGTTGGAGCAGTAGGCTTGATTTTGGAGGCCTCTTCCCTCTTCTCCAACTCCATAAGTGCTTTGGGTTTGCCTGCCGTTCCAGTTCTGGCCGTGATCTTCTTCCATGAAAAAATGAATGGAATCAAGGGAATAGCCATGGTTTTGGGCATTTGGGGATTTGTTTCGTACGCGTACCATCACTACATCGACGATCGTAAGTCCAAGACGGAGAATAGAAACGCCGGAAATGATGAAGCTTCGAAAGCTTTAAGGGTTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

394

Amino Acids

42.76

Weight (kDa)

8.88

Isoelectric Point (pI)

26.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 46 - 373 4.7e-122 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 901
AccB1I GGYRCC 1 cut(s) 218
AccI GTMKAC 1 cut(s) 350
AccII CGCG 5 cut(s) 112, 488, 512, 578, 1091
AciI CCGC 4 cut(s) 110, 112, 672, 816
AclI AACGTT 1 cut(s) 188
AclWI GGATC 1 cut(s) 605
AcoI YGGCCR 2 cut(s) 172, 1008
AcsI RAATTY 3 cut(s) 67, 538, 883
AcuI CTGAAG 2 cut(s) 5, 66
AcyI GRCGYC 2 cut(s) 123, 219
AfaI GTAC 4 cut(s) 206, 249, 1088, 1094
AfeI AGCGCT 1 cut(s) 747
AfiI CCNNNNNNNGG 2 cut(s) 620, 717
AflIII ACRYGT 2 cut(s) 486, 1089
AgsI TTSAA 4 cut(s) 484, 715, 835, 1178
AluBI AGCT 6 cut(s) 11, 25, 706, 859, 1157, 1166
AluI AGCT 6 cut(s) 11, 25, 706, 859, 1157, 1166
Alw26I GTCTC 2 cut(s) 382, 728
AlwI GGATC 1 cut(s) 605
Aor51HI AGCGCT 1 cut(s) 747
AoxI GGCC 5 cut(s) 77, 172, 477, 948, 1008
ApeKI GCWGC 1 cut(s) 106
ApoI RAATTY 3 cut(s) 67, 538, 883
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AspLEI GCGC 4 cut(s) 117, 221, 514, 748
AspS9I GGNCC 1 cut(s) 77
AsuHPI GGTGA 3 cut(s) 547, 599, 851
AsuII TTCGAA 1 cut(s) 1160
AsuNHI GCTAGC 1 cut(s) 812
BanI GGYRCC 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 597
BbvI GCAGC 1 cut(s) 93
BccI CCATC 4 cut(s) 343, 566, 838, 1104
BceAI ACGGC 2 cut(s) 980, 995
BciVI GTATCC 2 cut(s) 671, 880
BcoDI GTCTC 2 cut(s) 382, 728
BfaI CTAG 3 cut(s) 401, 813, 860
BfmI CTRYAG 1 cut(s) 660
BfoI RGCGCY 2 cut(s) 222, 749
BfuAI ACCTGC 1 cut(s) 901
BfuI GTATCC 2 cut(s) 671, 880
BisI GCNGC 3 cut(s) 107, 110, 113
BlpI GCTNAGC 1 cut(s) 26
BlsI GCNGC 3 cut(s) 108, 111, 114
BmcAI AGTACT 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 2 cut(s) 220, 650
BmsI GCATC 4 cut(s) 76, 374, 476, 677
BmtI GCTAGC 1 cut(s) 816
BpiI GAAGAC 1 cut(s) 597
BpmI CTGGAG 1 cut(s) 621
Bpu1102I GCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 1160
BsaBI GATNNNNATC 1 cut(s) 140
BsaHI GRCGYC 2 cut(s) 123, 219
BsaJI CCNNGG 4 cut(s) 110, 583, 795, 1056
Bsc4I CCNNNNNNNGG 2 cut(s) 620, 717
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 3 cut(s) 134, 175, 1001
Bse3DI GCAATG 1 cut(s) 165
Bse8I GATNNNNATC 1 cut(s) 140
BseDI CCNNGG 4 cut(s) 110, 583, 795, 1056
BseGI GGATG 2 cut(s) 288, 668
BseJI GATNNNNATC 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 620, 717
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 3 cut(s) 40, 485, 716
BseNI ACTGG 3 cut(s) 134, 175, 1001
BseRI GAGGAG 3 cut(s) 542, 553, 563
BseXI GCAGC 1 cut(s) 93
BseYI CCCAGC 1 cut(s) 292
Bsh1236I CGCG 5 cut(s) 112, 488, 512, 578, 1091
Bsh1285I CGRYCG 2 cut(s) 97, 1114
BshFI GGCC 5 cut(s) 79, 174, 479, 950, 1010
BshNI GGYRCC 1 cut(s) 218
BsiEI CGRYCG 2 cut(s) 97, 1114
BsiSI CCGG 3 cut(s) 171, 675, 1143
BsiWI CGTACG 1 cut(s) 1086
BslI CCNNNNNNNGG 2 cut(s) 620, 717
BsmAI GTCTC 2 cut(s) 382, 728
BsmBI CGTCTC 1 cut(s) 728
BsnI GGCC 5 cut(s) 79, 174, 479, 950, 1010
Bsp119I TTCGAA 1 cut(s) 1160
Bsp143I GATC 4 cut(s) 98, 610, 1014, 1111
Bsp1720I GCTNAGC 1 cut(s) 26
Bsp19I CCATGG 1 cut(s) 1056
BspACI CCGC 4 cut(s) 110, 112, 672, 816
BspANI GGCC 5 cut(s) 79, 174, 479, 950, 1010
BspCNI CTCAG 3 cut(s) 39, 484, 715
BspFNI CGCG 5 cut(s) 112, 488, 512, 578, 1091
BspLI GGNNCC 2 cut(s) 220, 650
BspMI ACCTGC 1 cut(s) 901
BspOI GCTAGC 1 cut(s) 816
BspPI GGATC 1 cut(s) 605
BspT104I TTCGAA 1 cut(s) 1160
BspT107I GGYRCC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 170
BsrI ACTGG 3 cut(s) 134, 175, 1001
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 4 cut(s) 110, 583, 795, 1056
BssMI GATC 4 cut(s) 98, 610, 1014, 1111
BssNI GRCGYC 2 cut(s) 123, 219
BssT1I CCWWGG 2 cut(s) 583, 1056
Bst4CI ACNGT 5 cut(s) 204, 244, 348, 661, 796
Bst6I CTCTTC 2 cut(s) 958, 965
BstACI GRCGYC 2 cut(s) 123, 219
BstBI TTCGAA 1 cut(s) 1160
BstC8I GCNNGC 6 cut(s) 172, 237, 298, 708, 814, 993
BstDEI CTNAG 3 cut(s) 26, 471, 702
BstDSI CCRYGG 3 cut(s) 110, 795, 1056
BstENI CCTNNNNNAGG 1 cut(s) 715
BstF5I GGATG 2 cut(s) 288, 668
BstFNI CGCG 5 cut(s) 112, 488, 512, 578, 1091
BstH2I RGCGCY 2 cut(s) 222, 749
BstHHI GCGC 4 cut(s) 117, 221, 514, 748
BstKTI GATC 4 cut(s) 101, 613, 1017, 1114
BstMAI GTCTC 2 cut(s) 382, 728
BstMBI GATC 4 cut(s) 98, 610, 1014, 1111
BstMCI CGRYCG 2 cut(s) 97, 1114
BstMWI GCNNNNNNNGC 4 cut(s) 112, 225, 485, 1163
BstSFI CTRYAG 1 cut(s) 660
BstUI CGCG 5 cut(s) 112, 488, 512, 578, 1091
BstV1I GCAGC 1 cut(s) 93
BstV2I GAAGAC 1 cut(s) 597
BstXI CCANNNNNNTGG 1 cut(s) 1063
BsuI GTATCC 2 cut(s) 671, 880
BsuRI GGCC 5 cut(s) 79, 174, 479, 950, 1010
BtgI CCRYGG 3 cut(s) 110, 795, 1056
BtgZI GCGATG 1 cut(s) 621
BtsCI GGATG 2 cut(s) 288, 668
BtsI GCAGTG 2 cut(s) 101, 522
BtsIMutI CAGTG 3 cut(s) 101, 522, 657
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 6 cut(s) 172, 237, 298, 708, 814, 993
CfoI GCGC 4 cut(s) 117, 221, 514, 748
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 1 cut(s) 77
Cfr42I CCGCGG 1 cut(s) 113
CseI GACGC 1 cut(s) 112
Csp6I GTAC 4 cut(s) 205, 248, 1087, 1093
CviAII CATG 7 cut(s) 271, 751, 757, 891, 906, 1025, 1057
CviQI GTAC 4 cut(s) 205, 248, 1087, 1093
DdeI CTNAG 3 cut(s) 26, 471, 702
DinI GGCGCC 1 cut(s) 220
DpnI GATC 4 cut(s) 100, 612, 1016, 1113
DpnII GATC 4 cut(s) 98, 610, 1014, 1111
EaeI YGGCCR 2 cut(s) 172, 1008
Eam1104I CTCTTC 2 cut(s) 958, 965
EarI CTCTTC 2 cut(s) 958, 965
EciI GGCGGA 1 cut(s) 661
Eco130I CCWWGG 2 cut(s) 583, 1056
Eco147I AGGCCT 1 cut(s) 950
Eco47III AGCGCT 1 cut(s) 747
Eco57I CTGAAG 2 cut(s) 5, 66
EcoNI CCTNNNNNAGG 1 cut(s) 715
EcoO109I RGGNCCY 1 cut(s) 77
EcoRI GAATTC 1 cut(s) 538
EcoT14I CCWWGG 2 cut(s) 583, 1056
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
ErhI CCWWGG 2 cut(s) 583, 1056
Esp3I CGTCTC 1 cut(s) 728
FaeI CATG 7 cut(s) 274, 754, 760, 894, 909, 1028, 1060
FatI CATG 7 cut(s) 270, 750, 756, 890, 905, 1024, 1056
FblI GTMKAC 1 cut(s) 350
Fnu4HI GCNGC 3 cut(s) 107, 110, 113
FokI GGATG 2 cut(s) 275, 655
Fsp4HI GCNGC 3 cut(s) 107, 110, 113
FspBI CTAG 3 cut(s) 401, 813, 860
GlaI GCGC 4 cut(s) 116, 220, 513, 747
GluI GCNGC 3 cut(s) 107, 110, 113
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 621
HaeII RGCGCY 2 cut(s) 222, 749
HaeIII GGCC 5 cut(s) 79, 174, 479, 950, 1010
HapII CCGG 3 cut(s) 171, 675, 1143
HgaI GACGC 1 cut(s) 112
HhaI GCGC 4 cut(s) 117, 221, 514, 748
Hin1I GRCGYC 2 cut(s) 123, 219
Hin1II CATG 7 cut(s) 274, 754, 760, 894, 909, 1028, 1060
Hin6I GCGC 4 cut(s) 115, 219, 512, 746
HinP1I GCGC 4 cut(s) 115, 219, 512, 746
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 2 cut(s) 1155, 1164
HinfI GANTC 3 cut(s) 141, 596, 1041
HpaII CCGG 3 cut(s) 171, 675, 1143
HphI GGTGA 3 cut(s) 547, 599, 851
Hpy166II GTNNAC 3 cut(s) 127, 351, 522
Hpy188I TCNGA 3 cut(s) 85, 94, 803
Hpy188III TCNNGA 3 cut(s) 14, 391, 600
Hpy8I GTNNAC 3 cut(s) 127, 351, 522
Hpy99I CGWCG 2 cut(s) 365, 1112
HpyAV CCTTC 7 cut(s) 90, 446, 490, 622, 721, 829, 859
HpyCH4III ACNGT 5 cut(s) 204, 244, 348, 661, 796
HpyCH4IV ACGT 2 cut(s) 188, 737
HpyCH4V TGCA 5 cut(s) 185, 467, 641, 657, 782
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 225, 485, 1163
HpyF3I CTNAG 3 cut(s) 26, 471, 702
HpySE526I ACGT 2 cut(s) 188, 737
Hsp92I GRCGYC 2 cut(s) 123, 219
Hsp92II CATG 7 cut(s) 274, 754, 760, 894, 909, 1028, 1060
HspAI GCGC 4 cut(s) 115, 219, 512, 746
KasI GGCGCC 1 cut(s) 218
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
KspI CCGCGG 1 cut(s) 113
Kzo9I GATC 4 cut(s) 98, 610, 1014, 1111
LmnI GCTCC 1 cut(s) 926
Lsp1109I GCAGC 1 cut(s) 93
LweI GCATC 4 cut(s) 76, 374, 476, 677
MaeI CTAG 3 cut(s) 401, 813, 860
MaeII ACGT 2 cut(s) 188, 737
MalI GATC 4 cut(s) 100, 612, 1016, 1113
MboI GATC 4 cut(s) 98, 610, 1014, 1111
MboII GAAGA 7 cut(s) 68, 493, 602, 945, 952, 1009, 1012
MfeI CAATTG 1 cut(s) 229
MluCI AATT 4 cut(s) 67, 229, 538, 883
MluI ACGCGT 2 cut(s) 486, 1089
Mly113I GGCGCC 1 cut(s) 219
MmeI TCCRAC 5 cut(s) 117, 335, 781, 904, 990
MroNI GCCGGC 1 cut(s) 170
MseI TTAA 3 cut(s) 39, 686, 1169
MslI CAYNNNNRTG 2 cut(s) 755, 761
MspA1I CMGCKG 1 cut(s) 112
MspI CCGG 3 cut(s) 171, 675, 1143
MunI CAATTG 1 cut(s) 229
MvnI CGCG 5 cut(s) 112, 488, 512, 578, 1091
MwoI GCNNNNNNNGC 4 cut(s) 112, 225, 485, 1163
NaeI GCCGGC 1 cut(s) 172
NarI GGCGCC 1 cut(s) 219
NcoI CCATGG 1 cut(s) 1056
NdeII GATC 4 cut(s) 98, 610, 1014, 1111
NgoMIV GCCGGC 1 cut(s) 170
NheI GCTAGC 1 cut(s) 812
NlaIII CATG 7 cut(s) 274, 754, 760, 894, 909, 1028, 1060
NlaIV GGNNCC 2 cut(s) 220, 650
NspV TTCGAA 1 cut(s) 1160
PceI AGGCCT 1 cut(s) 950
PdiI GCCGGC 1 cut(s) 172
PfeI GAWTC 3 cut(s) 141, 596, 1041
Pfl23II CGTACG 1 cut(s) 1086
PkrI GCNGC 3 cut(s) 108, 111, 114
Ple19I CGATCG 1 cut(s) 1114
PluTI GGCGCC 1 cut(s) 222
Psp1406I AACGTT 1 cut(s) 188
PspFI CCCAGC 1 cut(s) 292
PspLI CGTACG 1 cut(s) 1086
PspN4I GGNNCC 2 cut(s) 220, 650
PspPI GGNCC 1 cut(s) 77
PvuI CGATCG 1 cut(s) 1114
RsaI GTAC 4 cut(s) 206, 249, 1088, 1094
RsaNI GTAC 4 cut(s) 205, 248, 1087, 1093
RseI CAYNNNNRTG 2 cut(s) 755, 761
SacII CCGCGG 1 cut(s) 113
SaqAI TTAA 3 cut(s) 39, 686, 1169
SatI GCNGC 3 cut(s) 107, 110, 113
Sau3AI GATC 4 cut(s) 98, 610, 1014, 1111
Sau96I GGNCC 1 cut(s) 77
ScaI AGTACT 1 cut(s) 206
SfaNI GCATC 4 cut(s) 76, 374, 476, 677
SfcI CTRYAG 1 cut(s) 660
SfoI GGCGCC 1 cut(s) 220
Sfr303I CCGCGG 1 cut(s) 113
SfuI TTCGAA 1 cut(s) 1160
SgrBI CCGCGG 1 cut(s) 113
SmiMI CAYNNNNRTG 2 cut(s) 755, 761
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
Sse9I AATT 4 cut(s) 67, 229, 538, 883
SseBI AGGCCT 1 cut(s) 950
SsiI CCGC 4 cut(s) 110, 112, 672, 816
SspDI GGCGCC 1 cut(s) 218
SspMI CTAG 3 cut(s) 401, 813, 860
StuI AGGCCT 1 cut(s) 950
StyI CCWWGG 2 cut(s) 583, 1056
TaaI ACNGT 5 cut(s) 204, 244, 348, 661, 796
TaiI ACGT 2 cut(s) 191, 740
TaqI TCGA 3 cut(s) 626, 1107, 1160
TaqII GACCGA 1 cut(s) 111
TasI AATT 4 cut(s) 67, 229, 538, 883
TatI WGTACW 2 cut(s) 204, 247
TauI GCSGC 2 cut(s) 112, 115
TfiI GAWTC 3 cut(s) 141, 596, 1041
Tru1I TTAA 3 cut(s) 39, 686, 1169
Tru9I TTAA 3 cut(s) 39, 686, 1169
TscAI CASTG 3 cut(s) 108, 529, 664
TseI GCWGC 1 cut(s) 106
TspDTI ATGAA 7 cut(s) 60, 75, 277, 896, 1041, 1049, 1167
TspGWI ACGGA 3 cut(s) 409, 696, 1142
TspRI CASTG 3 cut(s) 108, 529, 664
XagI CCTNNNNNAGG 1 cut(s) 715
XapI RAATTY 3 cut(s) 67, 538, 883
XcmI CCANNNNNNNNNTGG 1 cut(s) 979
XmiI GTMKAC 1 cut(s) 350
XspI CTAG 3 cut(s) 401, 813, 860
ZrmI AGTACT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.