MD10G1266100.v1.1

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002718
Physical Location & Seq
Reverse (-)
35911741 .. 35913477
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1266100.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGGAAGAAGCTCAAGAACTTCAACTCAGCATCGGGAAGGAAGCAAATGGAGAAAATGAAGCAAACTTATCAGGGCATACAGATGTCTCAGACCGATCACTGCTACCGCAGCGCAGAAGTGTCAACTGGTGGATTAGGATCGCCATTTACTCGTTCCTCGTCATTGCCGGCCAGTCGGTTGCAGCATTATTGGGAAGACAGTACTACGACAAAGGCGCCAAAAGTAATTGGCTGGCAACAGTAGTACAACTTTGTGGCTTCCCTATCATGCTTCCCTACTATTGCATCCCAGCAAGCAGAAATAATCCAATCCCAAAAGATAGTCCTATCCCATCAAAACCACCGTCTACCAAAATCCTCGCATCAGTATATGTCTCTCTTGGCTTCCTAATAGCCCTAGGCTGCTACTTGTATTCCGTAGGACTATCTTACCTTCCTGTATCTACTTTAACTCTCATTTGTGCATCTCAATTGGCCTTCAACGCGTTTTTCTCCTTCTTCCTCAACGCGCAAAAGTTCACTTCTTACATTGTCAATTCTCTAGTTCTCCTCATCATCTCCTCCACCCTCCTCGTGTTCCAAGGCGAAGACAACTCTGGAGGCGATGATCCAAGTGCAGGATCGAAGGCAAAGTATGCAATTGGGTTCATATGCACCTTAGGGGCATCAGCTGGATTCGGATTATTTCTTTCCCTAACTCAGCTTGTCTTCAGAAGGGTTATAAAAAAGGAGACGTTTAGAGCGGTCATGGACATGGTAGTGTGTGAAAATCTGGTTGCAACCTGTGTAACCGTGGTCGGACTTTTCGCTAGCGGAGAGTGGAAACATTTGAAGGGTGAGATGGAGGGGTATAAATTAGGGAAGATATCCTATGTGATGAATGTGATGTGGACAGCAATAGCATGGCAGCTTGTTTCTGTTGGATCGGTGGGCTTGATTCTGGAGGCCTCTTCCCTCTTCTCCAATTCCATAAGTGCTTTGGGTTTGCCTGCTGTTCCAGTTCTAGCTGTGATCTTCTTCCATGAAAAAATGAGTGGAATTAAGGGAATAGCCATGGTTTTGGGTATTTGGGGATTTGTTTCGTACGCGTATCATCACTACATCGAAGATCGTAAGTCCAAGACGGAGAATAGAAATTCCGGGAATGTTGAAGTTTCAAAAGCTTTAAATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

392

Amino Acids

42.62

Weight (kDa)

8.55

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 45 - 372 7.8e-120 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 722
AccB1I GGYRCC 1 cut(s) 215
AccBSI CCGCTC 1 cut(s) 743
AccI GTMKAC 1 cut(s) 347
AccII CGCG 3 cut(s) 485, 509, 1088
AciI CCGC 3 cut(s) 107, 743, 813
AclWI GGATC 4 cut(s) 146, 602, 628, 931
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 1135
AcuI CTGAAG 1 cut(s) 694
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 3 cut(s) 203, 246, 1085
AfiI CCNNNNNNNGG 1 cut(s) 617
AflIII ACRYGT 2 cut(s) 483, 1086
AgsI TTSAA 5 cut(s) 23, 481, 832, 1151, 1158
AluBI AGCT 6 cut(s) 11, 671, 703, 910, 1007, 1163
AluI AGCT 6 cut(s) 11, 671, 703, 910, 1007, 1163
Alw26I GTCTC 3 cut(s) 91, 379, 725
AlwI GGATC 4 cut(s) 146, 602, 628, 931
AoxI GGCC 3 cut(s) 169, 474, 945
ApeKI GCWGC 4 cut(s) 109, 182, 402, 907
ApoI RAATTY 1 cut(s) 1135
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AspA2I CCTAGG 1 cut(s) 397
AspLEI GCGC 3 cut(s) 114, 218, 511
AsuC2I CCSGG 1 cut(s) 1141
AsuHPI GGTGA 1 cut(s) 848
AsuNHI GCTAGC 1 cut(s) 809
AvrII CCTAGG 1 cut(s) 397
AxyI CCTNAGG 1 cut(s) 658
BanI GGYRCC 1 cut(s) 215
BauI CACGAG 1 cut(s) 572
BbsI GAAGAC 3 cut(s) 202, 594, 700
BbvI GCAGC 4 cut(s) 121, 194, 389, 919
BccI CCATC 2 cut(s) 340, 835
BcnI CCSGG 1 cut(s) 1141
BcoDI GTCTC 3 cut(s) 91, 379, 725
BfaI CTAG 4 cut(s) 398, 542, 810, 1004
BfoI RGCGCY 1 cut(s) 219
BisI GCNGC 4 cut(s) 110, 183, 403, 908
BlnI CCTAGG 1 cut(s) 397
BlsI GCNGC 4 cut(s) 111, 184, 404, 909
BmcAI AGTACT 1 cut(s) 203
Bme1390I CCNGG 1 cut(s) 1141
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 1 cut(s) 1141
BmsI GCATC 5 cut(s) 39, 294, 371, 473, 674
BmtI GCTAGC 1 cut(s) 813
BpiI GAAGAC 3 cut(s) 202, 594, 700
BpmI CTGGAG 2 cut(s) 618, 962
BpuMI CCSGG 1 cut(s) 1141
BsaBI GATNNNNATC 1 cut(s) 137
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 4 cut(s) 397, 580, 792, 1053
Bsc4I CCNNNNNNNGG 1 cut(s) 617
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 3 cut(s) 131, 172, 998
Bse21I CCTNAGG 1 cut(s) 658
Bse3DI GCAATG 1 cut(s) 162
Bse8I GATNNNNATC 1 cut(s) 137
BseDI CCNNGG 4 cut(s) 397, 580, 792, 1053
BseGI GGATG 1 cut(s) 285
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 1 cut(s) 617
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 3 cut(s) 40, 102, 713
BseNI ACTGG 3 cut(s) 131, 172, 998
BseRI GAGGAG 3 cut(s) 539, 550, 560
BseXI GCAGC 4 cut(s) 121, 194, 389, 919
BseYI CCCAGC 1 cut(s) 289
BsgI GTGCAG 1 cut(s) 636
Bsh1236I CGCG 3 cut(s) 485, 509, 1088
BshFI GGCC 3 cut(s) 171, 476, 947
BshNI GGYRCC 1 cut(s) 215
BsiSI CCGG 2 cut(s) 168, 1140
BsiWI CGTACG 1 cut(s) 1083
BslI CCNNNNNNNGG 1 cut(s) 617
BsmAI GTCTC 3 cut(s) 91, 379, 725
BsmBI CGTCTC 1 cut(s) 725
BsnI GGCC 3 cut(s) 171, 476, 947
Bsp143I GATC 7 cut(s) 95, 138, 607, 620, 923, 1011, 1108
Bsp19I CCATGG 1 cut(s) 1053
BspACI CCGC 3 cut(s) 107, 743, 813
BspANI GGCC 3 cut(s) 171, 476, 947
BspCNI CTCAG 3 cut(s) 39, 101, 712
BspFNI CGCG 3 cut(s) 485, 509, 1088
BspLI GGNNCC 1 cut(s) 217
BspOI GCTAGC 1 cut(s) 813
BspPI GGATC 4 cut(s) 146, 602, 628, 931
BspT107I GGYRCC 1 cut(s) 215
BsrBI CCGCTC 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 3 cut(s) 131, 172, 998
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 4 cut(s) 397, 580, 792, 1053
BssMI GATC 7 cut(s) 95, 138, 607, 620, 923, 1011, 1108
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 1 cut(s) 572
BssT1I CCWWGG 3 cut(s) 397, 580, 1053
Bst2BI CACGAG 1 cut(s) 572
Bst4CI ACNGT 4 cut(s) 201, 241, 345, 793
Bst6I CTCTTC 2 cut(s) 955, 962
BstACI GRCGYC 1 cut(s) 216
BstAPI GCANNNNNTGC 1 cut(s) 635
BstC8I GCNNGC 5 cut(s) 169, 234, 295, 811, 990
BstDEI CTNAG 4 cut(s) 26, 88, 658, 699
BstDSI CCRYGG 2 cut(s) 792, 1053
BstF5I GGATG 1 cut(s) 285
BstFNI CGCG 3 cut(s) 485, 509, 1088
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 3 cut(s) 114, 218, 511
BstKTI GATC 7 cut(s) 98, 141, 610, 623, 926, 1014, 1111
BstMAI GTCTC 3 cut(s) 91, 379, 725
BstMBI GATC 7 cut(s) 95, 138, 607, 620, 923, 1011, 1108
BstMWI GCNNNNNNNGC 3 cut(s) 109, 482, 635
BstSCI CCNGG 1 cut(s) 1139
BstUI CGCG 3 cut(s) 485, 509, 1088
BstV1I GCAGC 4 cut(s) 121, 194, 389, 919
BstV2I GAAGAC 3 cut(s) 202, 594, 700
BstXI CCANNNNNNTGG 1 cut(s) 1060
Bsu36I CCTNAGG 1 cut(s) 658
BsuRI GGCC 3 cut(s) 171, 476, 947
BtgI CCRYGG 2 cut(s) 792, 1053
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 1 cut(s) 285
BtsI GCAGTG 1 cut(s) 98
BtsIMutI CAGTG 1 cut(s) 98
Cac8I GCNNGC 5 cut(s) 169, 234, 295, 811, 990
CfoI GCGC 3 cut(s) 114, 218, 511
Cfr10I RCCGGY 1 cut(s) 167
Csp6I GTAC 3 cut(s) 202, 245, 1084
CviAII CATG 6 cut(s) 268, 748, 754, 903, 1022, 1054
CviQI GTAC 3 cut(s) 202, 245, 1084
DdeI CTNAG 4 cut(s) 26, 88, 658, 699
DinI GGCGCC 1 cut(s) 217
DpnI GATC 7 cut(s) 97, 140, 609, 622, 925, 1013, 1110
DpnII GATC 7 cut(s) 95, 138, 607, 620, 923, 1011, 1108
DraI TTTAAA 1 cut(s) 1167
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 2 cut(s) 955, 962
EarI CTCTTC 2 cut(s) 955, 962
Eco130I CCWWGG 3 cut(s) 397, 580, 1053
Eco147I AGGCCT 1 cut(s) 947
Eco32I GATATC 1 cut(s) 867
Eco57I CTGAAG 1 cut(s) 694
Eco81I CCTNAGG 1 cut(s) 658
EcoRV GATATC 1 cut(s) 867
EcoT14I CCWWGG 3 cut(s) 397, 580, 1053
EgeI GGCGCC 1 cut(s) 217
EheI GGCGCC 1 cut(s) 217
ErhI CCWWGG 3 cut(s) 397, 580, 1053
Esp3I CGTCTC 1 cut(s) 725
FaeI CATG 6 cut(s) 271, 751, 757, 906, 1025, 1057
FatI CATG 6 cut(s) 267, 747, 753, 902, 1021, 1053
FauNDI CATATG 1 cut(s) 650
FblI GTMKAC 1 cut(s) 347
Fnu4HI GCNGC 4 cut(s) 110, 183, 403, 908
FokI GGATG 1 cut(s) 272
Fsp4HI GCNGC 4 cut(s) 110, 183, 403, 908
FspBI CTAG 4 cut(s) 398, 542, 810, 1004
GlaI GCGC 3 cut(s) 113, 217, 510
GluI GCNGC 4 cut(s) 110, 183, 403, 908
GsaI CCCAGC 1 cut(s) 293
GsuI CTGGAG 2 cut(s) 618, 962
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 3 cut(s) 171, 476, 947
HapII CCGG 2 cut(s) 168, 1140
HhaI GCGC 3 cut(s) 114, 218, 511
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 6 cut(s) 271, 751, 757, 906, 1025, 1057
Hin6I GCGC 3 cut(s) 112, 216, 509
HinP1I GCGC 3 cut(s) 112, 216, 509
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 1161
HinfI GANTC 2 cut(s) 675, 937
HpaII CCGG 2 cut(s) 168, 1140
HphI GGTGA 1 cut(s) 848
Hpy166II GTNNAC 4 cut(s) 124, 348, 519, 891
Hpy188I TCNGA 4 cut(s) 91, 680, 713, 800
Hpy188III TCNNGA 4 cut(s) 14, 34, 597, 941
Hpy8I GTNNAC 4 cut(s) 124, 348, 519, 891
HpyAV CCTTC 7 cut(s) 31, 443, 487, 505, 619, 708, 826
HpyCH4III ACNGT 4 cut(s) 201, 241, 345, 793
HpyCH4IV ACGT 1 cut(s) 734
HpyCH4V TGCA 7 cut(s) 182, 285, 464, 617, 638, 654, 779
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 482, 635
HpyF3I CTNAG 4 cut(s) 26, 88, 658, 699
HpySE526I ACGT 1 cut(s) 734
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 6 cut(s) 271, 751, 757, 906, 1025, 1057
HspAI GCGC 3 cut(s) 112, 216, 509
KasI GGCGCC 1 cut(s) 215
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 7 cut(s) 95, 138, 607, 620, 923, 1011, 1108
Lsp1109I GCAGC 4 cut(s) 121, 194, 389, 919
LweI GCATC 5 cut(s) 39, 294, 371, 473, 674
MaeI CTAG 4 cut(s) 398, 542, 810, 1004
MaeII ACGT 1 cut(s) 734
MaeIII GTNAC 1 cut(s) 787
MalI GATC 7 cut(s) 97, 140, 609, 622, 925, 1013, 1110
MbiI CCGCTC 1 cut(s) 743
MboI GATC 7 cut(s) 95, 138, 607, 620, 923, 1011, 1108
MfeI CAATTG 2 cut(s) 470, 639
MluCI AATT 8 cut(s) 226, 470, 535, 639, 854, 964, 1038, 1135
MluI ACGCGT 2 cut(s) 483, 1086
Mly113I GGCGCC 1 cut(s) 216
MmeI TCCRAC 2 cut(s) 778, 901
MroNI GCCGGC 1 cut(s) 167
MseI TTAA 3 cut(s) 449, 1041, 1166
MslI CAYNNNNRTG 3 cut(s) 81, 752, 758
MspA1I CMGCKG 1 cut(s) 671
MspI CCGG 2 cut(s) 168, 1140
MspR9I CCNGG 1 cut(s) 1141
MunI CAATTG 2 cut(s) 470, 639
MvnI CGCG 3 cut(s) 485, 509, 1088
MwoI GCNNNNNNNGC 3 cut(s) 109, 482, 635
NaeI GCCGGC 1 cut(s) 169
NarI GGCGCC 1 cut(s) 216
NciI CCSGG 1 cut(s) 1141
NcoI CCATGG 1 cut(s) 1053
NdeI CATATG 1 cut(s) 650
NdeII GATC 7 cut(s) 95, 138, 607, 620, 923, 1011, 1108
NgoMIV GCCGGC 1 cut(s) 167
NheI GCTAGC 1 cut(s) 809
NlaIII CATG 6 cut(s) 271, 751, 757, 906, 1025, 1057
NlaIV GGNNCC 1 cut(s) 217
PceI AGGCCT 1 cut(s) 947
PdiI GCCGGC 1 cut(s) 169
PfeI GAWTC 2 cut(s) 675, 937
Pfl23II CGTACG 1 cut(s) 1083
PfoI TCCNGGA 1 cut(s) 1139
PkrI GCNGC 4 cut(s) 111, 184, 404, 909
PluTI GGCGCC 1 cut(s) 219
PsiI TTATAA 1 cut(s) 722
PspFI CCCAGC 1 cut(s) 289
PspLI CGTACG 1 cut(s) 1083
PspN4I GGNNCC 1 cut(s) 217
PvuII CAGCTG 1 cut(s) 671
RsaI GTAC 3 cut(s) 203, 246, 1085
RsaNI GTAC 3 cut(s) 202, 245, 1084
RseI CAYNNNNRTG 3 cut(s) 81, 752, 758
SaqAI TTAA 3 cut(s) 449, 1041, 1166
SatI GCNGC 4 cut(s) 110, 183, 403, 908
Sau3AI GATC 7 cut(s) 95, 138, 607, 620, 923, 1011, 1108
ScaI AGTACT 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 1141
SfaNI GCATC 5 cut(s) 39, 294, 371, 473, 674
SfoI GGCGCC 1 cut(s) 217
SmiMI CAYNNNNRTG 3 cut(s) 81, 752, 758
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
Sse9I AATT 8 cut(s) 226, 470, 535, 639, 854, 964, 1038, 1135
SseBI AGGCCT 1 cut(s) 947
SsiI CCGC 3 cut(s) 107, 743, 813
SspDI GGCGCC 1 cut(s) 215
SspMI CTAG 4 cut(s) 398, 542, 810, 1004
StuI AGGCCT 1 cut(s) 947
StyD4I CCNGG 1 cut(s) 1139
StyI CCWWGG 3 cut(s) 397, 580, 1053
TaaI ACNGT 4 cut(s) 201, 241, 345, 793
TaiI ACGT 1 cut(s) 737
TaqI TCGA 2 cut(s) 623, 1104
TaqII GACCGA 1 cut(s) 108
TasI AATT 8 cut(s) 226, 470, 535, 639, 854, 964, 1038, 1135
TatI WGTACW 2 cut(s) 201, 244
TfiI GAWTC 2 cut(s) 675, 937
Tru1I TTAA 3 cut(s) 449, 1041, 1166
Tru9I TTAA 3 cut(s) 449, 1041, 1166
TscAI CASTG 1 cut(s) 105
TseI GCWGC 4 cut(s) 109, 182, 402, 907
TspDTI ATGAA 4 cut(s) 72, 637, 893, 1038
TspGWI ACGGA 2 cut(s) 406, 1139
TspRI CASTG 1 cut(s) 105
XapI RAATTY 1 cut(s) 1135
XcmI CCANNNNNNNNNTGG 1 cut(s) 976
XmaJI CCTAGG 1 cut(s) 397
XmiI GTMKAC 1 cut(s) 347
XspI CTAG 4 cut(s) 398, 542, 810, 1004
ZrmI AGTACT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.