AT4G18220

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002718
Physical Location & Seq
Forward (+)
10078638 .. 10080362
1725 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18220.1

Sequence Viewer

Length: 1173 bp
ATGAAGGGGGATCAAGAAGTACAAGTCATTGTTCAGCAGGGAAAAGAACCAATTCCAACAGATCAAGACGAAAGAAGTTCGGTCAGTGGCAGCCAAACAAAACTATCTCACTCTAATACATACAAACGGTGGCTCCGGGTTGCTATCTATACATTCTTTGTCATTTCAGGCCAATCAGTTGCTACAATTCTGGGCAGACTATACTATGAAAATGGAGGAAACAGCAAATGGCTAGCAACGGTAGTTCAGCTTGTAGGCTTTCCTATTCTACTTCCATATCATCTCTTGTCAGTCAAAACACATACAACAACTCAGAGAGATGGCAAATTAACCTCACTTAGGAACCGTGCATTAGTTTACATAGTGCTTGGACTTCTTGTAGGAGCAGCTTGCTACCTATATTCCATTGGACTGCTTTACCTACCTGTTTCTACCCTTTCCCTGATCTGTGCATCACAGTTAGCCTTCACCGCTTTCTTCTCTTATTTACTCAACTCACAAAAACTTACTCCTATCATTTTGAATTCTCTTTTCCTCCTCACTATATCTTCCACCCTCCTTGCATTTAATAACGAGGAATCAGATTCCAAAAAAGTTACAAAAGGAGAGTATGTCAAAGGTTTCGTATGCACCGTTGGTGCATCTGCTGGGTTTGGTCTACTCTTATCCCTACAACAGCTAGCCTTTCGTAAAGTTTTAAAGAAGCAAACTTTCTCAGAAGTTATAAATATGATAATCTACATGAGTCTAGTGGCCAGTTGTGTTAGCGTGGTGGGGCTTTTTGCTAGTAGCGAGTGGAAAACTTTGAGCAGTGAAATGGAAAACTACAAACTTGGGAAGGTATCCTATGTCATGAACCTAGTGTGGACAGCTGTTACCTGGCAGGTATTCTCCATCGGTTGCACAGGACTGATCTTCGAGCTTTCCTCCCTATTCTCAAATGCAATAAGCGCTTTGGGACTCCCCGTGGTTCCTATCCTGGCTGTCATCATTTTCCATGACAAAATGAACGGCTTAAAGGTGATTTCTATGATTCTAGCTATTTGGGGTTTCGTATCCTATGTCTACCAACAATATCTTGATGAAACAAACTTGAAGAAAAGTAATGAAATACCAACAACAGAATCCCCTGACCGACCAGAAGCAGAAGGGTCAAGTGAGCAATCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

390

Amino Acids

43.09

Weight (kDa)

8.88

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 46 - 362 1.2e-122 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 725
Acc36I ACCTGC 1 cut(s) 874
AccI GTMKAC 2 cut(s) 658, 1065
AciI CCGC 1 cut(s) 471
AclWI GGATC 1 cut(s) 18
AcoI YGGCCR 1 cut(s) 753
AcsI RAATTY 1 cut(s) 523
AfaI GTAC 1 cut(s) 21
AfeI AGCGCT 1 cut(s) 952
AfiI CCNNNNNNNGG 1 cut(s) 339
AgsI TTSAA 2 cut(s) 523, 1096
AjnI CCWGG 2 cut(s) 878, 978
AluBI AGCT 6 cut(s) 250, 389, 679, 872, 922, 1040
AluI AGCT 6 cut(s) 250, 389, 679, 872, 922, 1040
AlwI GGATC 1 cut(s) 18
Aor51HI AGCGCT 1 cut(s) 952
AoxI GGCC 2 cut(s) 169, 753
ApeKI GCWGC 2 cut(s) 90, 386
ApoI RAATTY 1 cut(s) 523
Asp700I GAANNNNTTC 1 cut(s) 51
AspLEI GCGC 1 cut(s) 953
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 2 cut(s) 460, 1033
AsuNHI GCTAGC 2 cut(s) 232, 679
BalI TGGCCA 1 cut(s) 755
BbvI GCAGC 2 cut(s) 102, 398
BccI CCATC 2 cut(s) 314, 902
BceAI ACGGC 1 cut(s) 1027
BciT130I CCWGG 2 cut(s) 880, 980
BciVI GTATCC 2 cut(s) 853, 1066
BcnI CCSGG 1 cut(s) 137
BfaI CTAG 6 cut(s) 233, 680, 749, 786, 860, 1037
BfoI RGCGCY 1 cut(s) 954
BfuAI ACCTGC 1 cut(s) 874
BfuI GTATCC 2 cut(s) 853, 1066
BisI GCNGC 2 cut(s) 91, 387
BlsI GCNGC 2 cut(s) 92, 388
Bme1390I CCNGG 3 cut(s) 137, 880, 980
BmiI GGNNCC 3 cut(s) 134, 344, 972
BmrFI CCNGG 3 cut(s) 137, 880, 980
BmsI GCATC 2 cut(s) 461, 650
BmtI GCTAGC 2 cut(s) 236, 683
BplI GAGNNNNNCTC 2 cut(s) 911, 943
BpuMI CCSGG 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 966
BsaXI ACNNNNNCTCC 4 cut(s) 117, 147, 597, 627
Bsc4I CCNNNNNNNGG 1 cut(s) 339
Bse1I ACTGG 1 cut(s) 756
BseBI CCWGG 2 cut(s) 880, 980
BseDI CCNNGG 1 cut(s) 966
BseLI CCNNNNNNNGG 1 cut(s) 339
BseMII CTCAG 2 cut(s) 326, 729
BseNI ACTGG 1 cut(s) 756
BseRI GAGGAG 1 cut(s) 527
BseXI GCAGC 2 cut(s) 102, 398
BseYI CCCAGC 1 cut(s) 647
BshFI GGCC 2 cut(s) 171, 755
BsiSI CCGG 1 cut(s) 136
BslFI GGGAC 1 cut(s) 972
BslI CCNNNNNNNGG 1 cut(s) 339
BsmFI GGGAC 1 cut(s) 972
BsnI GGCC 2 cut(s) 171, 755
Bsp143I GATC 4 cut(s) 10, 61, 444, 912
BspACI CCGC 1 cut(s) 471
BspANI GGCC 2 cut(s) 171, 755
BspCNI CTCAG 2 cut(s) 325, 728
BspHI TCATGA 1 cut(s) 852
BspLI GGNNCC 3 cut(s) 134, 344, 972
BspMI ACCTGC 1 cut(s) 874
BspOI GCTAGC 2 cut(s) 236, 683
BspPI GGATC 1 cut(s) 18
BsrI ACTGG 1 cut(s) 756
BssECI CCNNGG 1 cut(s) 966
BssMI GATC 4 cut(s) 10, 61, 444, 912
Bst2UI CCWGG 2 cut(s) 880, 980
Bst4CI ACNGT 5 cut(s) 129, 241, 347, 459, 634
BstC8I GCNNGC 3 cut(s) 234, 391, 681
BstDEI CTNAG 3 cut(s) 312, 338, 715
BstDSI CCRYGG 1 cut(s) 966
BstENI CCTNNNNNAGG 1 cut(s) 337
BstH2I RGCGCY 1 cut(s) 954
BstHHI GCGC 1 cut(s) 953
BstKTI GATC 4 cut(s) 13, 64, 447, 915
BstMBI GATC 4 cut(s) 10, 61, 444, 912
BstMWI GCNNNNNNNGC 2 cut(s) 470, 950
BstNI CCWGG 2 cut(s) 880, 980
BstSCI CCNGG 3 cut(s) 135, 878, 978
BstV1I GCAGC 2 cut(s) 102, 398
BsuI GTATCC 2 cut(s) 853, 1066
BsuRI GGCC 2 cut(s) 171, 755
BtgI CCRYGG 1 cut(s) 966
BtsI GCAGTG 1 cut(s) 817
BtsIMutI CAGTG 2 cut(s) 91, 817
BveI ACCTGC 1 cut(s) 874
Cac8I GCNNGC 3 cut(s) 234, 391, 681
CciI TCATGA 1 cut(s) 852
CfoI GCGC 1 cut(s) 953
Csp6I GTAC 1 cut(s) 20
CspCI CAANNNNNGTGG 2 cut(s) 541, 576
CviAII CATG 3 cut(s) 742, 853, 998
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 3 cut(s) 312, 338, 715
DpnI GATC 4 cut(s) 12, 63, 446, 914
DpnII GATC 4 cut(s) 10, 61, 444, 912
DraI TTTAAA 1 cut(s) 699
EaeI YGGCCR 1 cut(s) 753
Eco47III AGCGCT 1 cut(s) 952
EcoNI CCTNNNNNAGG 1 cut(s) 337
EcoRI GAATTC 1 cut(s) 523
EcoRII CCWGG 2 cut(s) 878, 978
FaeI CATG 3 cut(s) 745, 856, 1001
FaqI GGGAC 1 cut(s) 972
FatI CATG 3 cut(s) 741, 852, 997
FblI GTMKAC 2 cut(s) 658, 1065
Fnu4HI GCNGC 2 cut(s) 91, 387
Fsp4HI GCNGC 2 cut(s) 91, 387
FspBI CTAG 6 cut(s) 233, 680, 749, 786, 860, 1037
GlaI GCGC 1 cut(s) 952
GluI GCNGC 2 cut(s) 91, 387
GsaI CCCAGC 1 cut(s) 651
HaeII RGCGCY 1 cut(s) 954
HaeIII GGCC 2 cut(s) 171, 755
HapII CCGG 1 cut(s) 136
HhaI GCGC 1 cut(s) 953
Hin1II CATG 3 cut(s) 745, 856, 1001
Hin6I GCGC 1 cut(s) 951
HinP1I GCGC 1 cut(s) 951
HinfI GANTC 6 cut(s) 578, 584, 745, 960, 1033, 1124
HpaII CCGG 1 cut(s) 136
HphI GGTGA 2 cut(s) 460, 1033
Hpy166II GTNNAC 4 cut(s) 358, 659, 867, 1066
Hpy188I TCNGA 3 cut(s) 315, 583, 718
Hpy188III TCNNGA 4 cut(s) 14, 65, 853, 1079
Hpy8I GTNNAC 4 cut(s) 358, 659, 867, 1066
HpyAV CCTTC 3 cut(s) 475, 832, 1142
HpyCH4III ACNGT 5 cut(s) 129, 241, 347, 459, 634
HpyCH4V TGCA 7 cut(s) 350, 452, 563, 630, 641, 903, 944
HpyF10VI GCNNNNNNNGC 2 cut(s) 470, 950
HpyF3I CTNAG 3 cut(s) 312, 338, 715
Hsp92II CATG 3 cut(s) 745, 856, 1001
HspAI GCGC 1 cut(s) 951
Kzo9I GATC 4 cut(s) 10, 61, 444, 912
LmnI GCTCC 2 cut(s) 138, 383
Lsp1109I GCAGC 2 cut(s) 102, 398
LweI GCATC 2 cut(s) 461, 650
MaeI CTAG 6 cut(s) 233, 680, 749, 786, 860, 1037
MaeIII GTNAC 2 cut(s) 595, 874
MalI GATC 4 cut(s) 12, 63, 446, 914
MboI GATC 4 cut(s) 10, 61, 444, 912
MboII GAAGA 4 cut(s) 469, 540, 907, 1108
MlsI TGGCCA 1 cut(s) 755
MluCI AATT 4 cut(s) 51, 186, 326, 523
MluNI TGGCCA 1 cut(s) 755
MlyI GAGTC 2 cut(s) 754, 954
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 7 cut(s) 209, 343, 545, 548, 566, 568, 937
Mox20I TGGCCA 1 cut(s) 755
MroXI GAANNNNTTC 1 cut(s) 51
MscI TGGCCA 1 cut(s) 755
MseI TTAA 4 cut(s) 329, 567, 698, 1016
Msp20I TGGCCA 1 cut(s) 755
MspA1I CMGCKG 1 cut(s) 872
MspI CCGG 1 cut(s) 136
MspR9I CCNGG 3 cut(s) 137, 880, 980
MvaI CCWGG 2 cut(s) 880, 980
MwoI GCNNNNNNNGC 2 cut(s) 470, 950
NciI CCSGG 1 cut(s) 137
NdeII GATC 4 cut(s) 10, 61, 444, 912
NheI GCTAGC 2 cut(s) 232, 679
NlaIII CATG 3 cut(s) 745, 856, 1001
NlaIV GGNNCC 3 cut(s) 134, 344, 972
PagI TCATGA 1 cut(s) 852
PcsI WCGNNNNNNNCGW 1 cut(s) 630
PdmI GAANNNNTTC 1 cut(s) 51
PfeI GAWTC 4 cut(s) 578, 584, 1033, 1124
PkrI GCNGC 2 cut(s) 92, 388
PleI GAGTC 2 cut(s) 753, 954
PpsI GAGTC 2 cut(s) 753, 954
PsiI TTATAA 1 cut(s) 725
Psp6I CCWGG 2 cut(s) 878, 978
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 2 cut(s) 878, 978
PspN4I GGNNCC 3 cut(s) 134, 344, 972
PvuII CAGCTG 1 cut(s) 872
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
SaqAI TTAA 4 cut(s) 329, 567, 698, 1016
SatI GCNGC 2 cut(s) 91, 387
Sau3AI GATC 4 cut(s) 10, 61, 444, 912
SchI GAGTC 2 cut(s) 754, 954
ScrFI CCNGG 3 cut(s) 137, 880, 980
SfaNI GCATC 2 cut(s) 461, 650
Sse9I AATT 4 cut(s) 51, 186, 326, 523
SsiI CCGC 1 cut(s) 471
SspMI CTAG 6 cut(s) 233, 680, 749, 786, 860, 1037
StyD4I CCNGG 3 cut(s) 135, 878, 978
TaaI ACNGT 5 cut(s) 129, 241, 347, 459, 634
TaqI TCGA 1 cut(s) 918
TaqII GACCGA 2 cut(s) 70, 1149
TasI AATT 4 cut(s) 51, 186, 326, 523
TatI WGTACW 1 cut(s) 19
TfiI GAWTC 4 cut(s) 578, 584, 1033, 1124
Tru1I TTAA 4 cut(s) 329, 567, 698, 1016
Tru9I TTAA 4 cut(s) 329, 567, 698, 1016
TscAI CASTG 2 cut(s) 91, 817
TseI GCWGC 2 cut(s) 90, 386
TspDTI ATGAA 6 cut(s) 17, 222, 869, 1022, 1098, 1122
TspRI CASTG 2 cut(s) 91, 817
XagI CCTNNNNNAGG 1 cut(s) 337
XapI RAATTY 1 cut(s) 523
XmiI GTMKAC 2 cut(s) 658, 1065
XmnI GAANNNNTTC 1 cut(s) 51
XspI CTAG 6 cut(s) 233, 680, 749, 786, 860, 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.