FvH4_3g06171

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002718
Physical Location & Seq
Reverse (-)
3549405 .. 3552150
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06171.t1

Sequence Viewer

Length: 1287 bp
ATGGGAGAAGTAGAAGCTCAAGAACTGCAGCTGAGCATCAAGGTTCAAGAAGCAAATGAAACAAACTCCCAACCAGATGTTCCTAATAACCAGCTGTCTCGGAGCAGAGGTTTCAACTGGTGGCTTAGAATATCGATTTACACAGTCTTTGTCCTTGCTGGCCAGGCAATTGCAACACTCTTGGGAAGACAGTACTATGAAAAAGGTGGAAGCAGCAACTGGCTTGCAACACTAGTACAGCTTTGTGGCTTTCCTATCCTCCTTCCCTTTTATTTCATTCCAGCATTAAACAAAAACTGTCCAACCACAAATAGTACTACATCATTCGAGTCAAAGCCACCGTCTACATTAAAACTAGCATCAATTTATGCCTCTCTTGGCCTTCTTATAGCAGGAGACTGCTTCTTGTATTCAGTAGGATTATCTTACCTTCCTGTATCTACTTACTCCCTCATCTGCGCATCCCAACTCGCTTTCAATGCACTTTTCTCCTTCTTCCTCAACTCGCAGAAGTTCACTCCTTACATTGTCAACTCTCTTGTTGTCCTGACTATCTCATCCAGCCTCCTAGTGCTTCAAGGAGACGACTCTGCTGACTCGAGTAAAGTTTCCAGGCCCAAGTACATAATAGGCTTCATCTGCACAGTTGGAGCATCAGCTGGATATGCATTGTTGCTTTCCCTAACGCAGCTAATCTTCACAAAGGTTATAAAGAAAGAGACATTTAGAGTGATCATGGATATGATTGTGTTTCAATCAGTCTATTCATCCGCTATTATCTTGGTGGGATTTTTTGCTAGTGGAGACTGGAAGGGTATAAAGAATGAGATGGAGGGGTTTGAGTTAGGGAAGGTGTCATATATTATGAATTTAACATGGACAGCTATAGTGTGGCAGCTCTTTTCCTTAGGAATGGTGGGGTTAGTATTTGAGGCATCGGCCCTCTTCTCAAATTCAATAAGTGCTGTGGGTTTGCCTATTGTTCCAGTTCTAGCTGTGATCTTCTTCCATGACAAAATGGATGGGATAAAGGCAATGTCCATGGTTTTGGCTATTTGGGGATTCATTTCATATGTTTATCAGCACTACCTCGATGATCGGCCTCAAATCAATCCATGGATCTCCATGATCACCTGCAACAATATCAGGCATTTCATACTCCATATAGCTAGCATGGTCATTCCAAACTATCCCATGCTCAACTTCATGCTGATAACTGCCATCTATGTTTTGGTATCTCATAGGCTTTTTGAGTTAACGAATACGCTCAAGACAGTCTTTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

429

Amino Acids

47.62

Weight (kDa)

8.1

Isoelectric Point (pI)

29.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 43 - 367 1.6e-121 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
AarI CACCTGC 1 cut(s) 1142
Acc16I TGCGCA 1 cut(s) 460
Acc36I ACCTGC 1 cut(s) 1142
AccI GTMKAC 1 cut(s) 344
AciI CCGC 1 cut(s) 771
AclWI GGATC 1 cut(s) 1127
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 2 cut(s) 868, 952
AfaI GTAC 4 cut(s) 194, 237, 316, 623
AgsI TTSAA 6 cut(s) 47, 115, 478, 578, 755, 957
AhlI ACTAGT 1 cut(s) 232
AjnI CCWGG 2 cut(s) 162, 611
AjuI GAANNNNNNNTTGG 2 cut(s) 63, 95
Alw26I GTCTC 5 cut(s) 102, 390, 576, 713, 798
AlwI GGATC 1 cut(s) 1127
AlwNI CAGNNNCTG 1 cut(s) 219
Ama87I CYCGRG 1 cut(s) 598
AoxI GGCC 5 cut(s) 160, 379, 614, 939, 1100
ApeKI GCWGC 4 cut(s) 28, 213, 688, 895
ApoI RAATTY 2 cut(s) 868, 952
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AspLEI GCGC 1 cut(s) 461
AspS9I GGNCC 2 cut(s) 615, 940
AsuHPI GGTGA 1 cut(s) 1123
AsuNHI GCTAGC 1 cut(s) 1169
AvaI CYCGRG 1 cut(s) 598
AxyI CCTNAGG 1 cut(s) 907
BalI TGGCCA 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 193
BbvI GCAGC 4 cut(s) 40, 225, 700, 907
BccI CCATC 3 cut(s) 823, 1016, 1229
BciT130I CCWGG 2 cut(s) 164, 613
BclI TGATCA 2 cut(s) 732, 1128
BcoDI GTCTC 5 cut(s) 102, 390, 576, 713, 798
BcuI ACTAGT 1 cut(s) 232
BfaI CTAG 6 cut(s) 233, 356, 569, 798, 992, 1170
BfmI CTRYAG 2 cut(s) 26, 885
BfuAI ACCTGC 1 cut(s) 1142
BisI GCNGC 4 cut(s) 29, 214, 689, 896
BlpI GCTNAGC 1 cut(s) 32
BlsI GCNGC 4 cut(s) 30, 215, 690, 897
BmcAI AGTACT 2 cut(s) 194, 316
Bme1390I CCNGG 2 cut(s) 164, 613
BmeT110I CYCGRG 1 cut(s) 598
BmgT120I GGNCC 2 cut(s) 615, 940
BmrFI CCNGG 2 cut(s) 164, 613
BmsI GCATC 5 cut(s) 45, 368, 470, 662, 944
BmtI GCTAGC 1 cut(s) 1173
BpiI GAAGAC 1 cut(s) 193
Bpu1102I GCTNAGC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 1253
Bsa29I ATCGAT 1 cut(s) 134
BsaJI CCNNGG 2 cut(s) 1041, 1115
BsaXI ACNNNNNCTCC 2 cut(s) 94, 124
Bse1I ACTGG 4 cut(s) 122, 224, 812, 986
Bse21I CCTNAGG 1 cut(s) 907
Bse3DI GCAATG 1 cut(s) 1041
BseBI CCWGG 2 cut(s) 164, 613
BseCI ATCGAT 1 cut(s) 134
BseDI CCNNGG 2 cut(s) 1041, 1115
BseGI GGATG 4 cut(s) 461, 557, 767, 1027
BseMI GCAATG 1 cut(s) 1041
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 4 cut(s) 122, 224, 812, 986
BseXI GCAGC 4 cut(s) 40, 225, 700, 907
BsgI GTGCAG 1 cut(s) 625
BshFI GGCC 5 cut(s) 162, 381, 616, 941, 1102
BshVI ATCGAT 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 598
BsmAI GTCTC 5 cut(s) 102, 390, 576, 713, 798
BsmBI CGTCTC 1 cut(s) 576
BsnI GGCC 5 cut(s) 162, 381, 616, 941, 1102
BsoBI CYCGRG 1 cut(s) 598
Bsp143I GATC 5 cut(s) 732, 999, 1096, 1119, 1128
Bsp1720I GCTNAGC 1 cut(s) 32
Bsp19I CCATGG 2 cut(s) 1041, 1115
BspACI CCGC 1 cut(s) 771
BspANI GGCC 5 cut(s) 162, 381, 616, 941, 1102
BspCNI CTCAG 1 cut(s) 24
BspDI ATCGAT 1 cut(s) 134
BspMAI CTGCAG 1 cut(s) 30
BspMI ACCTGC 1 cut(s) 1142
BspOI GCTAGC 1 cut(s) 1173
BspPI GGATC 1 cut(s) 1127
BsrDI GCAATG 1 cut(s) 1041
BsrI ACTGG 4 cut(s) 122, 224, 812, 986
BssECI CCNNGG 2 cut(s) 1041, 1115
BssMI GATC 5 cut(s) 732, 999, 1096, 1119, 1128
BssT1I CCWWGG 2 cut(s) 1041, 1115
Bst2UI CCWGG 2 cut(s) 164, 613
Bst4CI ACNGT 6 cut(s) 145, 192, 299, 342, 646, 1276
Bst6I CTCTTC 1 cut(s) 950
BstC8I GCNNGC 3 cut(s) 160, 225, 1171
BstDEI CTNAG 3 cut(s) 32, 125, 907
BstDSI CCRYGG 2 cut(s) 1041, 1115
BstF5I GGATG 4 cut(s) 461, 557, 767, 1027
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 5 cut(s) 735, 1002, 1099, 1122, 1131
BstMAI GTCTC 5 cut(s) 102, 390, 576, 713, 798
BstMBI GATC 5 cut(s) 732, 999, 1096, 1119, 1128
BstMWI GCNNNNNNNGC 4 cut(s) 164, 479, 639, 665
BstNI CCWGG 2 cut(s) 164, 613
BstSCI CCNGG 2 cut(s) 162, 611
BstSFI CTRYAG 2 cut(s) 26, 885
BstV1I GCAGC 4 cut(s) 40, 225, 700, 907
BstV2I GAAGAC 1 cut(s) 193
BstX2I RGATCY 1 cut(s) 1119
BstXI CCANNNNNNTGG 1 cut(s) 1048
BstYI RGATCY 1 cut(s) 1119
Bsu15I ATCGAT 1 cut(s) 134
Bsu36I CCTNAGG 1 cut(s) 907
BsuRI GGCC 5 cut(s) 162, 381, 616, 941, 1102
BsuTUI ATCGAT 1 cut(s) 134
BtgI CCRYGG 2 cut(s) 1041, 1115
BtsCI GGATG 4 cut(s) 461, 557, 767, 1027
BveI ACCTGC 1 cut(s) 1142
Cac8I GCNNGC 3 cut(s) 160, 225, 1171
CaiI CAGNNNCTG 1 cut(s) 219
CfoI GCGC 1 cut(s) 461
Cfr13I GGNCC 2 cut(s) 615, 940
ClaI ATCGAT 1 cut(s) 134
Csp6I GTAC 4 cut(s) 193, 236, 315, 622
CviAII CATG 9 cut(s) 736, 876, 1010, 1042, 1116, 1126, 1174, 1195, 1207
CviQI GTAC 4 cut(s) 193, 236, 315, 622
DdeI CTNAG 3 cut(s) 32, 125, 907
DpnI GATC 5 cut(s) 734, 1001, 1098, 1121, 1130
DpnII GATC 5 cut(s) 732, 999, 1096, 1119, 1128
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 950
EarI CTCTTC 1 cut(s) 950
Eco130I CCWWGG 2 cut(s) 1041, 1115
Eco81I CCTNAGG 1 cut(s) 907
Eco88I CYCGRG 1 cut(s) 598
EcoRII CCWGG 2 cut(s) 162, 611
EcoT14I CCWWGG 2 cut(s) 1041, 1115
EcoT22I ATGCAT 1 cut(s) 670
ErhI CCWWGG 2 cut(s) 1041, 1115
Esp3I CGTCTC 1 cut(s) 576
FaeI CATG 9 cut(s) 739, 879, 1013, 1045, 1119, 1129, 1177, 1198, 1210
FalI AAGNNNNNCTT 1 cut(s) 1262
FatI CATG 9 cut(s) 735, 875, 1009, 1041, 1115, 1125, 1173, 1194, 1206
FauNDI CATATG 1 cut(s) 1072
FbaI TGATCA 2 cut(s) 732, 1128
FblI GTMKAC 1 cut(s) 344
Fnu4HI GCNGC 4 cut(s) 29, 214, 689, 896
FokI GGATG 4 cut(s) 448, 544, 754, 1034
Fsp4HI GCNGC 4 cut(s) 29, 214, 689, 896
FspBI CTAG 6 cut(s) 233, 356, 569, 798, 992, 1170
FspI TGCGCA 1 cut(s) 460
GlaI GCGC 1 cut(s) 460
GluI GCNGC 4 cut(s) 29, 214, 689, 896
HaeIII GGCC 5 cut(s) 162, 381, 616, 941, 1102
HhaI GCGC 1 cut(s) 461
Hin1II CATG 9 cut(s) 739, 879, 1013, 1045, 1119, 1129, 1177, 1198, 1210
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HincII GTYRAC 2 cut(s) 532, 1257
HindII GTYRAC 2 cut(s) 532, 1257
HinfI GANTC 4 cut(s) 329, 587, 596, 1062
HpaI GTTAAC 1 cut(s) 1257
HphI GGTGA 1 cut(s) 1123
Hpy166II GTNNAC 4 cut(s) 345, 516, 532, 1257
Hpy188I TCNGA 1 cut(s) 102
Hpy188III TCNNGA 4 cut(s) 20, 47, 547, 1270
Hpy8I GTNNAC 4 cut(s) 345, 516, 532, 1257
HpyAV CCTTC 6 cut(s) 272, 392, 440, 502, 805, 844
HpyCH4III ACNGT 6 cut(s) 145, 192, 299, 342, 646, 1276
HpyCH4V TGCA 7 cut(s) 28, 173, 227, 482, 642, 668, 1137
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 479, 639, 665
HpyF3I CTNAG 3 cut(s) 32, 125, 907
Hsp92II CATG 9 cut(s) 739, 879, 1013, 1045, 1119, 1129, 1177, 1198, 1210
HspAI GCGC 1 cut(s) 459
Ksp22I TGATCA 2 cut(s) 732, 1128
KspAI GTTAAC 1 cut(s) 1257
Kzo9I GATC 5 cut(s) 732, 999, 1096, 1119, 1128
LmnI GCTCC 2 cut(s) 102, 650
Lsp1109I GCAGC 4 cut(s) 40, 225, 700, 907
LweI GCATC 5 cut(s) 45, 368, 470, 662, 944
MaeI CTAG 6 cut(s) 233, 356, 569, 798, 992, 1170
MalI GATC 5 cut(s) 734, 1001, 1098, 1121, 1130
MboI GATC 5 cut(s) 732, 999, 1096, 1119, 1128
MboII GAAGA 6 cut(s) 198, 487, 688, 937, 994, 997
MfeI CAATTG 1 cut(s) 168
MflI RGATCY 1 cut(s) 1119
MlsI TGGCCA 1 cut(s) 162
MluCI AATT 4 cut(s) 168, 363, 868, 952
MluNI TGGCCA 1 cut(s) 162
MlyI GAGTC 3 cut(s) 338, 581, 590
MmeI TCCRAC 2 cut(s) 326, 628
Mox20I TGGCCA 1 cut(s) 162
Mph1103I ATGCAT 1 cut(s) 670
MscI TGGCCA 1 cut(s) 162
MseI TTAA 4 cut(s) 287, 350, 872, 1256
MslI CAYNNNNRTG 1 cut(s) 740
Msp20I TGGCCA 1 cut(s) 162
MspA1I CMGCKG 3 cut(s) 31, 94, 659
MspR9I CCNGG 2 cut(s) 164, 613
MunI CAATTG 1 cut(s) 168
MvaI CCWGG 2 cut(s) 164, 613
MwoI GCNNNNNNNGC 4 cut(s) 164, 479, 639, 665
NcoI CCATGG 2 cut(s) 1041, 1115
NdeI CATATG 1 cut(s) 1072
NdeII GATC 5 cut(s) 732, 999, 1096, 1119, 1128
NheI GCTAGC 1 cut(s) 1169
NlaIII CATG 9 cut(s) 739, 879, 1013, 1045, 1119, 1129, 1177, 1198, 1210
NsbI TGCGCA 1 cut(s) 460
NsiI ATGCAT 1 cut(s) 670
PaeR7I CTCGAG 1 cut(s) 598
PaqCI CACCTGC 1 cut(s) 1142
PfeI GAWTC 1 cut(s) 1062
PkrI GCNGC 4 cut(s) 30, 215, 690, 897
PleI GAGTC 3 cut(s) 337, 581, 590
PpsI GAGTC 3 cut(s) 337, 581, 590
PsiI TTATAA 1 cut(s) 710
Psp6I CCWGG 2 cut(s) 162, 611
PspGI CCWGG 2 cut(s) 162, 611
PspPI GGNCC 2 cut(s) 615, 940
PspXI VCTCGAGB 1 cut(s) 598
PstI CTGCAG 1 cut(s) 30
PstNI CAGNNNCTG 1 cut(s) 219
PsuI RGATCY 1 cut(s) 1119
PvuII CAGCTG 3 cut(s) 31, 94, 659
RsaI GTAC 4 cut(s) 194, 237, 316, 623
RsaNI GTAC 4 cut(s) 193, 236, 315, 622
RseI CAYNNNNRTG 1 cut(s) 740
SaqAI TTAA 4 cut(s) 287, 350, 872, 1256
SatI GCNGC 4 cut(s) 29, 214, 689, 896
Sau3AI GATC 5 cut(s) 732, 999, 1096, 1119, 1128
Sau96I GGNCC 2 cut(s) 615, 940
ScaI AGTACT 2 cut(s) 194, 316
SchI GAGTC 3 cut(s) 338, 581, 590
ScrFI CCNGG 2 cut(s) 164, 613
SfaNI GCATC 5 cut(s) 45, 368, 470, 662, 944
SfcI CTRYAG 2 cut(s) 26, 885
Sfr274I CTCGAG 1 cut(s) 598
SlaI CTCGAG 1 cut(s) 598
SmiMI CAYNNNNRTG 1 cut(s) 740
SmlI CTYRAG 3 cut(s) 18, 598, 1268
SmoI CTYRAG 3 cut(s) 18, 598, 1268
SpeI ACTAGT 1 cut(s) 232
Sse9I AATT 4 cut(s) 168, 363, 868, 952
SsiI CCGC 1 cut(s) 771
SspMI CTAG 6 cut(s) 233, 356, 569, 798, 992, 1170
StyD4I CCNGG 2 cut(s) 162, 611
StyI CCWWGG 2 cut(s) 1041, 1115
TaaI ACNGT 6 cut(s) 145, 192, 299, 342, 646, 1276
TaqI TCGA 4 cut(s) 134, 327, 599, 1092
TasI AATT 4 cut(s) 168, 363, 868, 952
TatI WGTACW 4 cut(s) 192, 235, 314, 621
TfiI GAWTC 1 cut(s) 1062
Tru1I TTAA 4 cut(s) 287, 350, 872, 1256
Tru9I TTAA 4 cut(s) 287, 350, 872, 1256
TseI GCWGC 4 cut(s) 28, 213, 688, 895
XapI RAATTY 2 cut(s) 868, 952
XcmI CCANNNNNNNNNTGG 1 cut(s) 1228
XhoI CTCGAG 1 cut(s) 598
XmiI GTMKAC 1 cut(s) 344
XspI CTAG 6 cut(s) 233, 356, 569, 798, 992, 1170
ZrmI AGTACT 2 cut(s) 194, 316
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.